URA2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	URA2
Entrez Gene ID	853311
Full Name	bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181563.2	NP_012405.2	bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAD	XP_001155357.1
Xenopus tropicalis (tropical clawed frog)	cad	XP_004920257.1
Canis lupus familiaris (dog)	CAD	XP_540124.3
Bos taurus (cattle)	CAD	NP_001178322.1
Gallus gallus (chicken)	CAD	XP_003641116.2
Kluyveromyces lactis	KLLA0E15357g	XP_454643.1
Eremothecium gossypii	AGOS_ACR263C	NP_983665.2
Neurospora crassa	NCU08287	XP_961813.1
Danio rerio (zebrafish)	cad	NP_001009884.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000300	XP_310823.5
Caenorhabditis elegans (roundworm)	pyr-1	NP_495838.2
Saccharomyces cerevisiae (baker's yeast)	URA2	NP_012405.2
Homo sapiens (human)	CAD	NP_004332.2
Rattus norvegicus (Norway rat)	Cad	NP_001099180.2
Drosophila melanogaster (fruit fly)	r	NP_523377.1
Schizosaccharomyces pombe (fission yeast)	ura1	NP_593055.1
	CAD	XP_002799221.1
Mus musculus (house mouse)	Cad	NP_076014.1
Magnaporthe oryzae (rice blast fungus)	MGG_01537	XP_003714510.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutation or inhibition of Hsp90 resulted in decreased accumulation of Ura2, indicating it is an Hsp90 client. Cpr6 interacted with Ura2 in the absence of stable Cpr6-Hsp90 interaction, suggesting a direct interaction.
Title: Interaction of heat shock protein 90 and the co-chaperone Cpr6 with Ura2, a bifunctional enzyme required for pyrimidine biosynthesis.

The following URA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the URA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02585	NM_001181563.2 Latest version!	Saccharomyces cerevisiae S288C bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase (URA2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

URA2 ( NM_001181563.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012405.2 Saccharomyces cerevisiae S288C bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase (URA2), partial mRNA.

Price & Availability↑

CloneID	OSi02585
Clone ID Related Accession (Same CDS sequence)	NM_001181563.2
Accession Version	NM_001181563.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). On Apr 26, 2011 this sequence version replaced NM_001181563.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCCACTA TTGCTCCCAC TGCTCCAATC ACCCCTCCAA TGGAATCTAC GGGTGACCGT 
CTGGTTACGT TGGAACTTAA GGACGGCACG GTCTTACAAG GTTATTCATT TGGTGCTGAG 
AAATCCGTCG CTGGTGAATT AGTTTTCCAA ACTGGTATGG TTGGTTATCC TGAATCTGTG 
ACCGATCCAT CTTATGAAGG CCAGATCTTA GTCATCACTT ACCCATTGGT AGGCAATTAT 
GGTGTCCCAG ATATGCACTT GAGAGATGAA TTGGTCGAAG AATTGCCAAG ATATTTTGAA 
AGTAATAGAA TCCATATTGC CGGTTTAGTT ATTTCTCACT ATACCGACGA GTACTCTCAT 
TATCTTGCTA AATCTTCCTT AGGTAAATGG TTACAAAATG AAGGGATCCC AGCTGTTTAT 
GGTGTTGATA CAAGATCATT GACCAAGCAT TTGAGAGATG CAGGTTCAAT GTTGGGTAGG 
TTGTCTTTGG AAAAAAGCGG CTCTGACAGA ACCATCTCCA GATCTTCCTC TTGGAGAAGT 
GCGTTTGATG TTCCTGAATG GGTGGATCCA AATGTTCAAA ACCTAGTTTC TAAGGTCTCC 
ATCAATGAAC CTAAATTGTA CGTTCCTCCA GCAGACAATA AGCACATCGA ATTGCAAACC 
GGACCCGATG GTAAAGTTTT AAGGATTCTA GCCATTGACG TGGGTATGAA ATACAATCAA 
ATTCGTTGTT TTATCAAAAG GGGTGTAGAA TTGAAAGTTG TTCCATGGAA CTACGATTTC 
ACTAAAGAAG ATTATGATGG TCTGTTTATT TCAAATGGTC CAGGTGATCC ATCTGTTCTA 
GATGATCTAT CTCAAAGATT GTCAAATGTC CTGGAGGCTA AAAAGACGCC AGTATTCGGT 
ATTTGTCTCG GTCATCAATT GATAGCAAGA GCTGCCGGTG CATCCACCCT AAAACTAAAA 
TTTGGTAACC GTGGTCATAA CATACCTTGT ACATCAACTA TAAGTGGTCG TTGTTACATA 
ACATCTCAAA ACCATGGGTT CGCTGTGGAT GTTGACACTC TAACTTCCGG ATGGAAGCCA 
TTGTTTGTTA ACGCGAATGA TGACTCTAAC GAAGGTATCT ATCATTCTGA ATTACCTTAT 
TTTTCCGTCC AATTCCATCC AGAATCCACA CCTGGCCCAA GAGATACAGA ATTCTTGTTT 
GACGTTTTTA TCCAAGCAGT TAAAGAATTC AAGTATACAC AAGTGTTGAA ACCAATCGCT 
TTCCCAGGTG GCCTATTAGA GGATAACGTT AAGGCACACC CTAGAATCGA AGCGAAGAAG 
GTTCTAGTTC TCGGTTCTGG TGGTTTGTCC ATCGGTCAAG CTGGTGAATT TGACTACTCT 
GGTTCTCAAG CCATCAAAGC TTTGAAAGAA GAAGGTATCT ATACAATTTT AATTAATCCA 
AACATTGCTA CCATCCAAAC TTCGAAAGGG TTGGCTGATA AGGTTTATTT TGTTCCTGTT 
ACTGCAGAGT TCGTGAGAAA AGTTATTTTA CATGAAAGGC CGGACGCCAT TTATGTGACA 
TTTGGTGGAC AAACTGCTTT ATCTGTTGGT ATAGCCATGA AAGATGAATT TGAGGCGTTA 
GGAGTTAAAG TATTGGGTAC TCCAATCGAT ACTATTATTA CCACGGAAGA CCGTGAACTC 
TTCAGTAATG CCATTGACGA AATTAATGAA AAATGTGCGA AATCTCAAGC TGCTAATTCA 
GTGGACGAGG CATTGGCCGC TGTCAAGGAG ATCGGTTTCC CAGTTATTGT ACGTGCTGCA 
TATGCATTGG GAGGTTTAGG TTCCGGTTTC GCTAATAATG AAAAAGAATT GGTTGATCTA 
TGTAATGTTG CATTTTCATC CTCACCTCAA GTTTTAGTCG AGAAATCTAT GAAAGGTTGG 
AAAGAAGTCG AATATGAAGT TGTTCGTGAT GCTTTTGACA ACTGTATTAC TGTTTGTAAT 
ATGGAAAATT TTGATCCACT AGGTATTCAC ACCGGTGATT CCATCGTTGT AGCCCCATCT 
CAAACTTTAT CTGATGAAGA TTACAATATG TTAAGAACTA CTGCTGTTAA TGTTATTAGA 
CACTTAGGTG TTGTTGGTGA ATGTAATATC CAATATGCTT TAAATCCCGT TTCTAAGGAT 
TATTGCATCA TTGAAGTTAA TGCGCGTTTG TCACGTTCCT CTGCTTTAGC TTCTAAGGCT 
ACTGGTTACC CATTGGCCTA CACTGCGGCT AAGTTAGGTT TGAATATCCC ATTAAATGAA 
GTTAAGAATT CCGTCACAAA ATCCACATGT GCTTGTTTTG AACCTTCTCT AGACTACTGT 
GTTGTCAAAA TGCCAAGATG GGATTTGAAG AAGTTCACCA GAGTTTCTAC CGAATTATCT 
TCATCAATGA AATCTGTTGG TGAAGTTATG AGCATCGGTA GAACCTTCGA AGAAGCTATT 
CAAAAAGCTA TCAGATCCAC AGAATATGCT AACCTCGGAT TCAATGAGAC AGATCTAGAT 
ATCGACATTG ATTACGAGTT GAACAACCCT ACGGATATGC GTGTCTTCGC CATTGCAAAT 
GCTTTTGCTA AGAAGGGATA TTCTGTTGAT AAAGTCTGGG AAATGACTAG AATTGATAAG 
TGGTTTTTAA ACAAACTGCA CGATTTGGTC CAATTTGCTG AGAAAATTAG TTCATTTGGT 
ACTAAAGAGG AATTACCTTC CTTAGTTTTA AGACAGGCTA AGCAGTTAGG TTTTGATGAC 
AGACAGATTG CAAGATTTTT GGATTCCAAC GAAGTTGCCA TCCGTAGATT AAGAAAAGAA 
TATGGAATTA CACCATTTGT CAAACAAATT GATACAGTTG CCGCTGAATT CCCCGCTTAC 
ACGAACTATT TATACATGAC ATACAATGCT GACTCACACG ATTTATCCTT TGATGACCAC 
GGTGTTATGG TCTTGGGTTC TGGTGTTTAC CGTATCGGTT CTTCTGTCGA ATTTGATTGG 
TGTGCTGTTA CTGCAGTTAG AACATTACGT GCCAACAATA TCAAAACTAT CATGGTCAAC 
TATAATCCAG AAACTGTTTC CACAGATTAT GATGAGGCTG ATAGACTATA CTTTGAAACC 
ATCAACCTTG AAAGAGTTTT GGATATTTAC GAGATTGAAA ACTCAAGCGG TGTTGTCGTA 
TCAATGGGTG GTCAAACTTC CAACAACATC GCCATGACTT TACATCGTGA AAATGTAAAG 
ATTCTTGGTA CATCCCCTGA CATGATTGAT TCTGCTGAAA ACCGTTACAA GTTCTCTCGT 
ATGTTGGATC AAATTGGTGT TGACCAACCA GCTTGGAAAG AATTGACATC CATGGACGAA 
GCTGAATCTT TTGCCGAAAA GGTGGGTTAT CCAGTTTTGG TACGTCCATC TTATGTGTTA 
TCCGGTGCCG CCATGAATAC TGTTTATTCT AAAAACGATT TGGAATCCTA CTTAAACCAA 
GCCGTCGAAG TTTCACGTGA TTATCCTGTT GTTATCACTA AATATATTGA AAACGCAAAG 
GAGATTGAAA TGGATGCAGT TGCAAGAAAT GGTGAATTGG TTATGCATGT TGTCTCTGAG 
CATGTTGAAA ATGCAGGTGT CCACTCGGGT GATGCAACAT TAATCGTTCC ACCTCAAGAT 
TTGGCTCCTG AAACTGTGGA TAGAATTGTT GTCGCCACTG CTAAAATTGG TAAGGCTTTG 
AAAATTACAG GTCCATACAA CATCCAATTC ATTGCAAAGG ACAATGAAAT CAAGGTCATA 
GAATGTAATG TTCGTGCTTC GAGATCTTTC CCATTCATTT CAAAGGTTGT TGGCGTCAAT 
CTGATTGAAT TGGCAACAAA GGCCATAATG GGTTTGCCTT TGACGCCTTA TCCTGTTGAA 
AAATTACCAG ATGATTATGT CGCCGTTAAA GTACCACAAT TCTCTTTCCC ACGTTTAGCA 
GGAGCTGATC CAGTCTTAGG TGTTGAAATG GCCTCTACTG GTGAAGTCGC TACTTTTGGC 
CACTCGAAGT ATGAAGCATA CTTAAAGTCT TTGTTGGCAA CCGGCTTCAA ACTTCCAAAG 
AAGAATATTT TATTGTCTAT TGGTTCTTAC AAGGAAAAAC AAGAATTGCT TTCTTCCGTA 
CAAAAACTAT ACAACATGGG ATATAAATTA TTTGCAACAT CAGGTACTGC TGACTTTTTA 
TCTGAACATG GTATTGCCGT CCAATATCTG GAGGTTTTAA ACAAGGATGA TGATGATCAA 
AAATCAGAAT ACTCACTTAC TCAACATTTG GCTAATAATG AAATTGACCT TTACATCAAC 
TTGCCTTCTG CCAACAGGTT CCGTCGTCCT GCATCCTATG TTTCAAAGGG GTATAAAACA 
CGTCGTTTGG CTGTCGATTA TTCGGTTCCG TTGGTTACTA ACGTTAAATG TGCAAAATTG 
TTGATTGAAG CCATTTCAAG AAATATCACT TTAGATGTTT CTGAACGTGA TGCACAAACT 
TCCCACAGAA CTATTACCTT ACCTGGTTTA ATCAATATCG CAACTTATGT TCCGAATGCA 
TCCCATGTTA TCAAAGGCCC AGCTGAACTG AAGGAGACCA CACGTCTATT TTTGGAATCC 
GGTTTTACAT ACTGTCAATT GATGCCCAGA TCCATCAGCG GACCTGTTAT TACTGACGTT 
GCATCCTTGA AGGCCGCAAA CTCTGTTTCC CAAGATTCAT CTTACACTGA CTTTTCTTTC 
ACTATTGCTG GTACAGCACA CAATGCTCAT AGTGTTACGC AATCTGCTAG CAAAGTGACA 
GCATTGTTTT TGCCCCTACG TGAATTAAAG AACAAGATCA CAGCAGTAGC TGAACTTCTG 
AATCAGTGGC CAACTGAAAA GCAAGTGATT GCAGAAGCTA AAACTGCAGA TTTGGCATCG 
GTTTTATTGT TAACCTCCCT TCAAAACAGA TCTATTCACA TTACTGGTGT TTCGAATAAG 
GAAGATTTAG CTTTGATCAT GACGGTCAAG GCAAAAGACC CTAGAGTGAC TTGTGATGTC 
AATATTTATT CTTTGTTTAT TGCCCAAGAT GACTATCCGG AGGCAGTTTT CTTGCCTACT 
AAGGAAGATC AAGAGTTTTT CTGGAACAAC CTTGATAGTA TCGATGCTTT CTCTGTCGGT 
GCTCTTCCTG TCGCCTTGGC AAATGTCACA GGTAACAAGG TTGATGTTGG TATGGGTATC 
AAAGATTCAT TACCACTATT GTTGGCTGCT GTTGAAGAAG GTAAATTAAC CATTGATGAT 
ATCGTCCTTC GTCTGCATGA CAATCCCGCT AAAATTTTCA ACATCCCTAC TCAGGACTCA 
GTTGTCGAAA TTGATTTGGA TTATTCTTTC AGACGTAATA AGAGATGGTC ACCATTCAAC 
AAAGATATGA ACGGTGGTAT TGAGCGTGTT GTCTATAATG GCGAAACATT AGTTTTGAGC 
GGTGAATTAG TTTCACCAGG CGCCAAAGGA AAATGCATTG TTAATCCAAG TCCAGCTTCC 
ATAACTGCTT CCGCAGAGCT CCAATCTACT AGTGCTAAAA GAAGGTTCTC GATCACGGAA 
GAAGCAATCG CTGATAATTT AGATGCCGCG GAGGATGCAA TTCCAGAACA GCCTTTGGAA 
CAAAAATTGA TGTCTTCAAG GCCACCAAGA GAACTTGTTG CTCCAGGCGC CATCCAGAAT 
TTGATCCGTA GTAACAATCC ATTCCGCGGA AGACATATAT TGTCTATCAA ACAATTCAAA 
CGTTCTGATT TCCATGTGTT GTTTGCTGTT GCACAAGAAC TAAGGGCAGC TGTCGCAAGA 
GAAGGTGTCT TAGATTTAAT GAAAGGCCAC GTTATTACTA CAATTTTCTT TGAACCATCT 
ACTCGTACTT GTTCTTCATT CATTGCTGCT ATGGAACGTT TGGGTGGTAG AATTGTAAAT 
GTTAATCCAT TGGTGTCTTC TGTCAAGAAA GGTGAAACCC TTCAAGATAC TATCAGAACT 
TTGGCTTGTT ACAGTGATGC CATTGTCATG CGTCATTCAG AAGAAATGTC TGTTCATATC 
GCCGCTAAAT ATTCTCCTGT TCCAATTATT AATGGTGGTA ATGGTTCTCG CGAGCATCCT 
ACGCAGGCCT TCTTGGATTT GTTTACGATT CGTGAAGAAA TCGGTACTGT TAATGGTATT 
ACTGTTACTT TCATGGGTGA TCTCAAACAT GGTAGAACCG TACATTCATT GTGTCGTTTG 
TTAATGCACT ATCAAGTCAG AATTAATCTT GTTTCTCCTC CGGAATTGAG GTTACCAGAA 
GGATTAAGAG AAGAGCTAAG AAAAGCTGGC TTACTTGGTG TTGAGAGCAT TGAATTAACC 
CCTCATATCA TCTCAAAGAC CGATGTTCTG TATTGTACAA GGGTCCAAGA AGAAAGATTC 
AATAGCCCTG AAGAATATGC ACGTCTGAAG GATACTTATA TCGTGGACAA CAAGATCTTG 
GCACACGCCA AAGAAAATAT GGCTATCATG CATCCATTGC CTCGTGTAAA TGAAATCAAA 
GAGGAAGTGG ACTACGATCA TCGTGCTGCT TACTTCAGAC AAATGAAGTA TGGTTTGTTC 
GTCAGAATGG CTTTGTTGGC CATGGTCATG GGTGTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012405.2
CDS	1..6645
Translation	MATIAPTAPITPPMESTGDRLVTLELKDGTVLQGYSFGAEKSVAGELVFQTGMVGYPESVTDPSYEGQILVITYPLVGNYGVPDMHLRDELVEELPRYFESNRIHIAGLVISHYTDEYSHYLAKSSLGKWLQNEGIPAVYGVDTRSLTKHLRDAGSMLGRLSLEKSGSDRTISRSSSWRSAFDVPEWVDPNVQNLVSKVSINEPKLYVPPADNKHIELQTGPDGKVLRILAIDVGMKYNQIRCFIKRGVELKVVPWNYDFTKEDYDGLFISNGPGDPSVLDDLSQRLSNVLEAKKTPVFGICLGHQLIARAAGASTLKLKFGNRGHNIPCTSTISGRCYITSQNHGFAVDVDTLTSGWKPLFVNANDDSNEGIYHSELPYFSVQFHPESTPGPRDTEFLFDVFIQAVKEFKYTQVLKPIAFPGGLLEDNVKAHPRIEAKKVLVLGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTILINPNIATIQTSKGLADKVYFVPVTAEFVRKVILHERPDAIYVTFGGQTALSVGIAMKDEFEALGVKVLGTPIDTIITTEDRELFSNAIDEINEKCAKSQAANSVDEALAAVKEIGFPVIVRAAYALGGLGSGFANNEKELVDLCNVAFSSSPQVLVEKSMKGWKEVEYEVVRDAFDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPVSKDYCIIEVNARLSRSSALASKATGYPLAYTAAKLGLNIPLNEVKNSVTKSTCACFEPSLDYCVVKMPRWDLKKFTRVSTELSSSMKSVGEVMSIGRTFEEAIQKAIRSTEYANLGFNETDLDIDIDYELNNPTDMRVFAIANAFAKKGYSVDKVWEMTRIDKWFLNKLHDLVQFAEKISSFGTKEELPSLVLRQAKQLGFDDRQIARFLDSNEVAIRRLRKEYGITPFVKQIDTVAAEFPAYTNYLYMTYNADSHDLSFDDHGVMVLGSGVYRIGSSVEFDWCAVTAVRTLRANNIKTIMVNYNPETVSTDYDEADRLYFETINLERVLDIYEIENSSGVVVSMGGQTSNNIAMTLHRENVKILGTSPDMIDSAENRYKFSRMLDQIGVDQPAWKELTSMDEAESFAEKVGYPVLVRPSYVLSGAAMNTVYSKNDLESYLNQAVEVSRDYPVVITKYIENAKEIEMDAVARNGELVMHVVSEHVENAGVHSGDATLIVPPQDLAPETVDRIVVATAKIGKALKITGPYNIQFIAKDNEIKVIECNVRASRSFPFISKVVGVNLIELATKAIMGLPLTPYPVEKLPDDYVAVKVPQFSFPRLAGADPVLGVEMASTGEVATFGHSKYEAYLKSLLATGFKLPKKNILLSIGSYKEKQELLSSVQKLYNMGYKLFATSGTADFLSEHGIAVQYLEVLNKDDDDQKSEYSLTQHLANNEIDLYINLPSANRFRRPASYVSKGYKTRRLAVDYSVPLVTNVKCAKLLIEAISRNITLDVSERDAQTSHRTITLPGLINIATYVPNASHVIKGPAELKETTRLFLESGFTYCQLMPRSISGPVITDVASLKAANSVSQDSSYTDFSFTIAGTAHNAHSVTQSASKVTALFLPLRELKNKITAVAELLNQWPTEKQVIAEAKTADLASVLLLTSLQNRSIHITGVSNKEDLALIMTVKAKDPRVTCDVNIYSLFIAQDDYPEAVFLPTKEDQEFFWNNLDSIDAFSVGALPVALANVTGNKVDVGMGIKDSLPLLLAAVEEGKLTIDDIVLRLHDNPAKIFNIPTQDSVVEIDLDYSFRRNKRWSPFNKDMNGGIERVVYNGETLVLSGELVSPGAKGKCIVNPSPASITASAELQSTSAKRRFSITEEAIADNLDAAEDAIPEQPLEQKLMSSRPPRELVAPGAIQNLIRSNNPFRGRHILSIKQFKRSDFHVLFAVAQELRAAVAREGVLDLMKGHVITTIFFEPSTRTCSSFIAAMERLGGRIVNVNPLVSSVKKGETLQDTIRTLACYSDAIVMRHSEEMSVHIAAKYSPVPIINGGNGSREHPTQAFLDLFTIREEIGTVNGITVTFMGDLKHGRTVHSLCRLLMHYQVRINLVSPPELRLPEGLREELRKAGLLGVESIELTPHIISKTDVLYCTRVQEERFNSPEEYARLKDTYIVDNKILAHAKENMAIMHPLPRVNEIKEEVDYDHRAAYFRQMKYGLFVRMALLAMVMGVDM

Target ORF information:

RefSeq Version	NM_001181563.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase (URA2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181563.2

ORF Insert Sequence:

ATGGCCACTA TTGCTCCCAC TGCTCCAATC ACCCCTCCAA TGGAATCTAC GGGTGACCGT 
CTGGTTACGT TGGAACTTAA GGACGGCACG GTCTTACAAG GTTATTCATT TGGTGCTGAG 
AAATCCGTCG CTGGTGAATT AGTTTTCCAA ACTGGTATGG TTGGTTATCC TGAATCTGTG 
ACCGATCCAT CTTATGAAGG CCAGATCTTA GTCATCACTT ACCCATTGGT AGGCAATTAT 
GGTGTCCCAG ATATGCACTT GAGAGATGAA TTGGTCGAAG AATTGCCAAG ATATTTTGAA 
AGTAATAGAA TCCATATTGC CGGTTTAGTT ATTTCTCACT ATACCGACGA GTACTCTCAT 
TATCTTGCTA AATCTTCCTT AGGTAAATGG TTACAAAATG AAGGGATCCC AGCTGTTTAT 
GGTGTTGATA CAAGATCATT GACCAAGCAT TTGAGAGATG CAGGTTCAAT GTTGGGTAGG 
TTGTCTTTGG AAAAAAGCGG CTCTGACAGA ACCATCTCCA GATCTTCCTC TTGGAGAAGT 
GCGTTTGATG TTCCTGAATG GGTGGATCCA AATGTTCAAA ACCTAGTTTC TAAGGTCTCC 
ATCAATGAAC CTAAATTGTA CGTTCCTCCA GCAGACAATA AGCACATCGA ATTGCAAACC 
GGACCCGATG GTAAAGTTTT AAGGATTCTA GCCATTGACG TGGGTATGAA ATACAATCAA 
ATTCGTTGTT TTATCAAAAG GGGTGTAGAA TTGAAAGTTG TTCCATGGAA CTACGATTTC 
ACTAAAGAAG ATTATGATGG TCTGTTTATT TCAAATGGTC CAGGTGATCC ATCTGTTCTA 
GATGATCTAT CTCAAAGATT GTCAAATGTC CTGGAGGCTA AAAAGACGCC AGTATTCGGT 
ATTTGTCTCG GTCATCAATT GATAGCAAGA GCTGCCGGTG CATCCACCCT AAAACTAAAA 
TTTGGTAACC GTGGTCATAA CATACCTTGT ACATCAACTA TAAGTGGTCG TTGTTACATA 
ACATCTCAAA ACCATGGGTT CGCTGTGGAT GTTGACACTC TAACTTCCGG ATGGAAGCCA 
TTGTTTGTTA ACGCGAATGA TGACTCTAAC GAAGGTATCT ATCATTCTGA ATTACCTTAT 
TTTTCCGTCC AATTCCATCC AGAATCCACA CCTGGCCCAA GAGATACAGA ATTCTTGTTT 
GACGTTTTTA TCCAAGCAGT TAAAGAATTC AAGTATACAC AAGTGTTGAA ACCAATCGCT 
TTCCCAGGTG GCCTATTAGA GGATAACGTT AAGGCACACC CTAGAATCGA AGCGAAGAAG 
GTTCTAGTTC TCGGTTCTGG TGGTTTGTCC ATCGGTCAAG CTGGTGAATT TGACTACTCT 
GGTTCTCAAG CCATCAAAGC TTTGAAAGAA GAAGGTATCT ATACAATTTT AATTAATCCA 
AACATTGCTA CCATCCAAAC TTCGAAAGGG TTGGCTGATA AGGTTTATTT TGTTCCTGTT 
ACTGCAGAGT TCGTGAGAAA AGTTATTTTA CATGAAAGGC CGGACGCCAT TTATGTGACA 
TTTGGTGGAC AAACTGCTTT ATCTGTTGGT ATAGCCATGA AAGATGAATT TGAGGCGTTA 
GGAGTTAAAG TATTGGGTAC TCCAATCGAT ACTATTATTA CCACGGAAGA CCGTGAACTC 
TTCAGTAATG CCATTGACGA AATTAATGAA AAATGTGCGA AATCTCAAGC TGCTAATTCA 
GTGGACGAGG CATTGGCCGC TGTCAAGGAG ATCGGTTTCC CAGTTATTGT ACGTGCTGCA 
TATGCATTGG GAGGTTTAGG TTCCGGTTTC GCTAATAATG AAAAAGAATT GGTTGATCTA 
TGTAATGTTG CATTTTCATC CTCACCTCAA GTTTTAGTCG AGAAATCTAT GAAAGGTTGG 
AAAGAAGTCG AATATGAAGT TGTTCGTGAT GCTTTTGACA ACTGTATTAC TGTTTGTAAT 
ATGGAAAATT TTGATCCACT AGGTATTCAC ACCGGTGATT CCATCGTTGT AGCCCCATCT 
CAAACTTTAT CTGATGAAGA TTACAATATG TTAAGAACTA CTGCTGTTAA TGTTATTAGA 
CACTTAGGTG TTGTTGGTGA ATGTAATATC CAATATGCTT TAAATCCCGT TTCTAAGGAT 
TATTGCATCA TTGAAGTTAA TGCGCGTTTG TCACGTTCCT CTGCTTTAGC TTCTAAGGCT 
ACTGGTTACC CATTGGCCTA CACTGCGGCT AAGTTAGGTT TGAATATCCC ATTAAATGAA 
GTTAAGAATT CCGTCACAAA ATCCACATGT GCTTGTTTTG AACCTTCTCT AGACTACTGT 
GTTGTCAAAA TGCCAAGATG GGATTTGAAG AAGTTCACCA GAGTTTCTAC CGAATTATCT 
TCATCAATGA AATCTGTTGG TGAAGTTATG AGCATCGGTA GAACCTTCGA AGAAGCTATT 
CAAAAAGCTA TCAGATCCAC AGAATATGCT AACCTCGGAT TCAATGAGAC AGATCTAGAT 
ATCGACATTG ATTACGAGTT GAACAACCCT ACGGATATGC GTGTCTTCGC CATTGCAAAT 
GCTTTTGCTA AGAAGGGATA TTCTGTTGAT AAAGTCTGGG AAATGACTAG AATTGATAAG 
TGGTTTTTAA ACAAACTGCA CGATTTGGTC CAATTTGCTG AGAAAATTAG TTCATTTGGT 
ACTAAAGAGG AATTACCTTC CTTAGTTTTA AGACAGGCTA AGCAGTTAGG TTTTGATGAC 
AGACAGATTG CAAGATTTTT GGATTCCAAC GAAGTTGCCA TCCGTAGATT AAGAAAAGAA 
TATGGAATTA CACCATTTGT CAAACAAATT GATACAGTTG CCGCTGAATT CCCCGCTTAC 
ACGAACTATT TATACATGAC ATACAATGCT GACTCACACG ATTTATCCTT TGATGACCAC 
GGTGTTATGG TCTTGGGTTC TGGTGTTTAC CGTATCGGTT CTTCTGTCGA ATTTGATTGG 
TGTGCTGTTA CTGCAGTTAG AACATTACGT GCCAACAATA TCAAAACTAT CATGGTCAAC 
TATAATCCAG AAACTGTTTC CACAGATTAT GATGAGGCTG ATAGACTATA CTTTGAAACC 
ATCAACCTTG AAAGAGTTTT GGATATTTAC GAGATTGAAA ACTCAAGCGG TGTTGTCGTA 
TCAATGGGTG GTCAAACTTC CAACAACATC GCCATGACTT TACATCGTGA AAATGTAAAG 
ATTCTTGGTA CATCCCCTGA CATGATTGAT TCTGCTGAAA ACCGTTACAA GTTCTCTCGT 
ATGTTGGATC AAATTGGTGT TGACCAACCA GCTTGGAAAG AATTGACATC CATGGACGAA 
GCTGAATCTT TTGCCGAAAA GGTGGGTTAT CCAGTTTTGG TACGTCCATC TTATGTGTTA 
TCCGGTGCCG CCATGAATAC TGTTTATTCT AAAAACGATT TGGAATCCTA CTTAAACCAA 
GCCGTCGAAG TTTCACGTGA TTATCCTGTT GTTATCACTA AATATATTGA AAACGCAAAG 
GAGATTGAAA TGGATGCAGT TGCAAGAAAT GGTGAATTGG TTATGCATGT TGTCTCTGAG 
CATGTTGAAA ATGCAGGTGT CCACTCGGGT GATGCAACAT TAATCGTTCC ACCTCAAGAT 
TTGGCTCCTG AAACTGTGGA TAGAATTGTT GTCGCCACTG CTAAAATTGG TAAGGCTTTG 
AAAATTACAG GTCCATACAA CATCCAATTC ATTGCAAAGG ACAATGAAAT CAAGGTCATA 
GAATGTAATG TTCGTGCTTC GAGATCTTTC CCATTCATTT CAAAGGTTGT TGGCGTCAAT 
CTGATTGAAT TGGCAACAAA GGCCATAATG GGTTTGCCTT TGACGCCTTA TCCTGTTGAA 
AAATTACCAG ATGATTATGT CGCCGTTAAA GTACCACAAT TCTCTTTCCC ACGTTTAGCA 
GGAGCTGATC CAGTCTTAGG TGTTGAAATG GCCTCTACTG GTGAAGTCGC TACTTTTGGC 
CACTCGAAGT ATGAAGCATA CTTAAAGTCT TTGTTGGCAA CCGGCTTCAA ACTTCCAAAG 
AAGAATATTT TATTGTCTAT TGGTTCTTAC AAGGAAAAAC AAGAATTGCT TTCTTCCGTA 
CAAAAACTAT ACAACATGGG ATATAAATTA TTTGCAACAT CAGGTACTGC TGACTTTTTA 
TCTGAACATG GTATTGCCGT CCAATATCTG GAGGTTTTAA ACAAGGATGA TGATGATCAA 
AAATCAGAAT ACTCACTTAC TCAACATTTG GCTAATAATG AAATTGACCT TTACATCAAC 
TTGCCTTCTG CCAACAGGTT CCGTCGTCCT GCATCCTATG TTTCAAAGGG GTATAAAACA 
CGTCGTTTGG CTGTCGATTA TTCGGTTCCG TTGGTTACTA ACGTTAAATG TGCAAAATTG 
TTGATTGAAG CCATTTCAAG AAATATCACT TTAGATGTTT CTGAACGTGA TGCACAAACT 
TCCCACAGAA CTATTACCTT ACCTGGTTTA ATCAATATCG CAACTTATGT TCCGAATGCA 
TCCCATGTTA TCAAAGGCCC AGCTGAACTG AAGGAGACCA CACGTCTATT TTTGGAATCC 
GGTTTTACAT ACTGTCAATT GATGCCCAGA TCCATCAGCG GACCTGTTAT TACTGACGTT 
GCATCCTTGA AGGCCGCAAA CTCTGTTTCC CAAGATTCAT CTTACACTGA CTTTTCTTTC 
ACTATTGCTG GTACAGCACA CAATGCTCAT AGTGTTACGC AATCTGCTAG CAAAGTGACA 
GCATTGTTTT TGCCCCTACG TGAATTAAAG AACAAGATCA CAGCAGTAGC TGAACTTCTG 
AATCAGTGGC CAACTGAAAA GCAAGTGATT GCAGAAGCTA AAACTGCAGA TTTGGCATCG 
GTTTTATTGT TAACCTCCCT TCAAAACAGA TCTATTCACA TTACTGGTGT TTCGAATAAG 
GAAGATTTAG CTTTGATCAT GACGGTCAAG GCAAAAGACC CTAGAGTGAC TTGTGATGTC 
AATATTTATT CTTTGTTTAT TGCCCAAGAT GACTATCCGG AGGCAGTTTT CTTGCCTACT 
AAGGAAGATC AAGAGTTTTT CTGGAACAAC CTTGATAGTA TCGATGCTTT CTCTGTCGGT 
GCTCTTCCTG TCGCCTTGGC AAATGTCACA GGTAACAAGG TTGATGTTGG TATGGGTATC 
AAAGATTCAT TACCACTATT GTTGGCTGCT GTTGAAGAAG GTAAATTAAC CATTGATGAT 
ATCGTCCTTC GTCTGCATGA CAATCCCGCT AAAATTTTCA ACATCCCTAC TCAGGACTCA 
GTTGTCGAAA TTGATTTGGA TTATTCTTTC AGACGTAATA AGAGATGGTC ACCATTCAAC 
AAAGATATGA ACGGTGGTAT TGAGCGTGTT GTCTATAATG GCGAAACATT AGTTTTGAGC 
GGTGAATTAG TTTCACCAGG CGCCAAAGGA AAATGCATTG TTAATCCAAG TCCAGCTTCC 
ATAACTGCTT CCGCAGAGCT CCAATCTACT AGTGCTAAAA GAAGGTTCTC GATCACGGAA 
GAAGCAATCG CTGATAATTT AGATGCCGCG GAGGATGCAA TTCCAGAACA GCCTTTGGAA 
CAAAAATTGA TGTCTTCAAG GCCACCAAGA GAACTTGTTG CTCCAGGCGC CATCCAGAAT 
TTGATCCGTA GTAACAATCC ATTCCGCGGA AGACATATAT TGTCTATCAA ACAATTCAAA 
CGTTCTGATT TCCATGTGTT GTTTGCTGTT GCACAAGAAC TAAGGGCAGC TGTCGCAAGA 
GAAGGTGTCT TAGATTTAAT GAAAGGCCAC GTTATTACTA CAATTTTCTT TGAACCATCT 
ACTCGTACTT GTTCTTCATT CATTGCTGCT ATGGAACGTT TGGGTGGTAG AATTGTAAAT 
GTTAATCCAT TGGTGTCTTC TGTCAAGAAA GGTGAAACCC TTCAAGATAC TATCAGAACT 
TTGGCTTGTT ACAGTGATGC CATTGTCATG CGTCATTCAG AAGAAATGTC TGTTCATATC 
GCCGCTAAAT ATTCTCCTGT TCCAATTATT AATGGTGGTA ATGGTTCTCG CGAGCATCCT 
ACGCAGGCCT TCTTGGATTT GTTTACGATT CGTGAAGAAA TCGGTACTGT TAATGGTATT 
ACTGTTACTT TCATGGGTGA TCTCAAACAT GGTAGAACCG TACATTCATT GTGTCGTTTG 
TTAATGCACT ATCAAGTCAG AATTAATCTT GTTTCTCCTC CGGAATTGAG GTTACCAGAA 
GGATTAAGAG AAGAGCTAAG AAAAGCTGGC TTACTTGGTG TTGAGAGCAT TGAATTAACC 
CCTCATATCA TCTCAAAGAC CGATGTTCTG TATTGTACAA GGGTCCAAGA AGAAAGATTC 
AATAGCCCTG AAGAATATGC ACGTCTGAAG GATACTTATA TCGTGGACAA CAAGATCTTG 
GCACACGCCA AAGAAAATAT GGCTATCATG CATCCATTGC CTCGTGTAAA TGAAATCAAA 
GAGGAAGTGG ACTACGATCA TCGTGCTGCT TACTTCAGAC AAATGAAGTA TGGTTTGTTC 
GTCAGAATGG CTTTGTTGGC CATGGTCATG GGTGTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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URA2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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