XRN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	XRN1
Entrez Gene ID	852702
Full Name	chromatin-binding exonuclease XRN1
Synonyms	DST2,KEM1,RAR5,SEP1,SKI1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181038.1	NP_011342.1	chromatin-binding exonuclease XRN1

Pathways from BioSystems

Homologies

Homo sapiens (human)	XRN1	NP_061874.3
Mus musculus (house mouse)	Xrn1	NP_036046.2
Rattus norvegicus (Norway rat)	Xrn1	XP_217233.5
Caenorhabditis elegans (roundworm)	xrn-1	NP_496945.3
Schizosaccharomyces pombe (fission yeast)	exo2	NP_593482.1
	XRN1	XP_001112305.1
Saccharomyces cerevisiae (baker's yeast)	XRN1	NP_011342.1
Magnaporthe oryzae (rice blast fungus)	MGG_12403	XP_003715558.1
Canis lupus familiaris (dog)	XRN1	XP_005634594.1
Danio rerio (zebrafish)	xrn1	NP_957327.2
Drosophila melanogaster (fruit fly)	pcm	NP_001162796.1
Eremothecium gossypii	AGOS_ADL046C	NP_984050.1
Neurospora crassa	NCU06678	XP_960925.2
Gallus gallus (chicken)	XRN1	XP_422596.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004430	XP_313715.5
Xenopus tropicalis (tropical clawed frog)	xrn1	XP_002937144.2
Pan troglodytes (chimpanzee)	XRN1	XP_001161640.1
Bos taurus (cattle)	XRN1	NP_001192964.1
Kluyveromyces lactis	KLLA0F22385g	XP_456080.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Xrn1 facilitates the generation of ssDNA and checkpoint activation at uncapped telomeres. Xrn1 also maintains telomere length independently of ssDNA generation by downregulating the amount of Rif1.
Title: Regulation of telomere metabolism by the RNA processing protein Xrn1.

Studies indicate important roles of the exoribonucleases DIS3L2 and XRN1 in cellular function, viability and disease.
Title: The roles of the exoribonucleases DIS3L2 and XRN1 in human disease.

Xrn1p co-evolves with totiviruses to maintain its potent antiviral activity and limit viral propagation in Saccharomyces yeasts
Title: XRN1 Is a Species-Specific Virus Restriction Factor in Yeasts.

the mechanism for intron turnover requires healing of the 5' termini of linear introns by the tRNA ligase Rlg1 and destruction by the cytoplasmic tRNA quality control 5'-to-3' exonuclease Xrn1, which has specificity for RNAs with 5' monophosphate
Title: Healing for destruction: tRNA intron degradation in yeast is a two-step cytoplasmic process catalyzed by tRNA ligase Rlg1 and 5'-to-3' exonuclease Xrn1.

Xrn1 is required for Snf1-dependent mRNA homeostasis in response to nutrient availability.
Title: Phosphoproteomic analysis identifies proteins involved in transcription-coupled mRNA decay as targets of Snf1 signaling.

The following XRN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the XRN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02079	NM_001181038.1 Latest version!	Saccharomyces cerevisiae S288C chromatin-binding exonuclease XRN1 (XRN1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

XRN1 ( NM_001181038.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011342.1 Saccharomyces cerevisiae S288C chromatin-binding exonuclease XRN1 (XRN1), partial mRNA.

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CloneID	OSi02079
Clone ID Related Accession (Same CDS sequence)	NM_001181038.1
Accession Version	NM_001181038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	chromatin-binding exonuclease XRN1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGTATTC CAAAATTTTT CAGGTACATC TCAGAAAGAT GGCCCATGAT TTTACAGCTT 
ATTGAGGGAA CACAGATTCC TGAGTTTGAT AACTTATACC TGGATATGAA TTCGATTTTA 
CATAATTGTA CGCATGGTAA CGACGATGAT GTAACCAAGC GATTAACTGA AGAAGAGGTT 
TTTGCAAAAA TCTGTACGTA TATCGATCAC CTTTTTCAAA CAATCAAGCC CAAGAAGATT 
TTCTACATGG CTATTGATGG TGTGGCCCCT CGTGCCAAGA TGAATCAACA AAGAGCTCGT 
AGATTCAGAA CCGCTATGGA TGCAGAAAAA GCCTTGAAGA AGGCTATTGA GAATGGTGAC 
GAGATTCCTA AGGGTGAGCC ATTTGATTCG AATTCTATTA CTCCAGGTAC GGAGTTTATG 
GCCAAATTGA CCAAAAACTT ACAATATTTT ATTCACGACA AGATTTCTAA CGATTCCAAA 
TGGAGGGAAG TGCAAATCAT ATTTTCTGGC CATGAAGTTC CAGGTGAAGG TGAACACAAG 
ATCATGAACT TTATAAGGCA TTTAAAATCC CAAAAGGATT TCAACCAGAA TACGAGACAT 
TGTATTTACG GTCTTGACGC AGATTTGATT ATGCTGGGTT TGTCTACTCA TGGGCCACAT 
TTTGCGTTAT TGAGAGAAGA AGTGACATTT GGTAGAAGAA ATAGTGAAAA AAAATCGCTT 
GAACATCAAA ATTTCTACTT ATTACATCTT TCTTTATTAA GAGAATACAT GGAGTTGGAA 
TTCAAAGAAA TTGCCGATGA AATGCAATTT GAATACAATT TTGAACGTAT TTTGGATGAT 
TTTATTCTTG TCATGTTCGT CATTGGTAAT GATTTCTTGC CCAATTTGCC AGATTTGCAC 
CTTAACAAAG GAGCATTTCC CGTTTTGTTA CAAACGTTCA AAGAAGCTCT TTTACATACT 
GATGGCTACA TTAATGAACA TGGTAAAATA AATTTAAAGA GATTAGGTGT CTGGTTAAAT 
TATCTGTCTC AATTTGAGTT ATTAAATTTC GAAAAGGATG ATATAGACGT TGAGTGGTTC 
AACAAGCAAT TAGAGAATAT TTCTTTGGAG GGTGAAAGGA AAAGACAGAG GGTTGGTAAA 
AAATTACTGG TAAAACAACA GAAGAAATTA ATTGGAAGTA TAAAACCATG GTTGATGGAG 
CAATTACAGG AAAAATTATC GCCTGATTTA CCAGATGAAG AAATTCCAAC TTTAGAGTTA 
CCTAAGGACT TAGACATGAA AGATCATTTA GAATTTTTAA AAGAATTCGC TTTTGATTTG 
GGTCTTTTTA TAACGCATTC CAAATCCAAA GGTAGTTATT CGCTAAAAAT GGATCTTGAT 
TCTATTAATC CTGATGAAAC AGAAGAAGAA TTTCAAAATC GTGTTAATTC TATCAGGAAA 
ACAATAAAAA AATATCAAAA TGCTATCATC GTGGAGGACA AAGAAGAATT GGAAACTGAA 
AAAACGATTT ATAATGAAAG GTTTGAACGT TGGAAGCATG AGTATTATCA CGACAAGTTA 
AAATTTACGA CAGACAGTGA AGAAAAAGTG AGAGATCTTG CTAAAGACTA CGTTGAAGGT 
TTACAATGGG TTCTATATTA TTATTATAGA GGATGTCCAT CTTGGTCGTG GTACTATCCG 
CACCATTATG CACCAAGAAT CTCCGACTTA GCCAAGGGTT TAGATCAAGA CATTGAATTT 
GATTTGAGCA AACCATTTAC TCCATTCCAG CAACTAATGG CAGTTTTACC GGAAAGGTCC 
AAAAATCTGA TACCTCCCGC CTTTAGGCCA TTAATGTACG ATGAACAGTC GCCAATCCAC 
GATTTCTATC CCGCTGAGGT TCAACTTGAT AAAAACGGCA AGACAGCTGA TTGGGAAGCT 
GTGGTTTTGA TATCGTTTGT AGATGAAAAA AGGTTGATTG AGGCTATGCA ACCTTATTTG 
CGCAAGTTAT CACCTGAAGA AAAAACGAGA AATCAATTTG GCAAGGACTT GATATATTCC 
TTTAATCCTC AAGTTGATAA CCTTTATAAG AGTCCGTTGG GCGGCATTTT TTCTGATATT 
GAACACAATC ATTGTGTCGA AAAAGAGTAC ATCACCATCC CATTGGACAG CTCCGAGATT 
CGGTATGGTT TATTACCTAA TGCTAAACTC GGTGCCGAAA TGCTGGCGGG TTTCCCCACG 
TTATTGTCTT TACCATTTAC TAGTTCACTG GAGTACAATG AGACAATGGT TTTCCAACAA 
CCTTCTAAAC AACAATCAAT GGTCTTACAA ATAACTGACA TATACAAAAC GAATAATGTT 
ACTTTGGAGG ACTTTTCCAA GAGGCATTTA AACAAAGTGA TTTATACAAG ATGGCCATAT 
TTAAGAGAAT CCAAATTGGT CTCTTTAACG GATGGTAAGA CTATCTATGA ATATCAGGAG 
TCCAATGATA AGAAAAAGTT CGGATTCATA ACGAAGCCTG CGGAAACCCA GGACAAAAAA 
CTTTTCAATA GTTTGAAGAA TTCAATGCTA AGGATGTATG CTAAACAGAA AGCTGTTAAA 
ATAGGACCTA TGGAAGCCAT TGCTACCGTC TTTCCAGTGA CTGGTTTGGT GAGAGACTCT 
GATGGTGGTT ATATTAAGAC CTTTAGCCCT ACCCCAGATT ACTATCCATT GCAACTGGTT 
GTTGAATCTG TTGTCAACGA GGATGAAAGA TATAAAGAAA GAGGACCCAT TCCTATTGAA 
GAGGAATTTC CATTGAATTC AAAAGTTATT TTCTTAGGTG ATTATGCCTA TGGTGGTGAA 
ACTACTATTG ACGGTTACAG CAGTGACCGC AGACTAAAAA TTACTGTAGA AAAGAAGTTT 
TTGGATAGTG AGCCCACCAT CGGCAAAGAA AGGTTACAAA TGGATCATCA AGCCGTTAAA 
TATTATCCGT CTTATATTGT GTCCAAGAAC ATGCACTTAC ACCCCTTGTT TTTGTCTAAG 
ATTACTTCCA AGTTCATGAT TACTGACGCT ACTGGGAAGC ATATCAATGT TGGTATCCCG 
GTTAAGTTCG AAGCTAGACA CCAAAAGGTT TTAGGTTACG CGAGGAGGAA CCCTAGGGGC 
TGGGAATACT CAAATTTAAC TCTAAATTTA CTGAAAGAGT ATAGACAAAC TTTCCCAGAT 
TTTTTTTTCA GGTTGTCCAA GGTAGGTAAT GATATCCCAG TTTTGGAAGA TCTTTTCCCC 
GATACTTCCA CTAAGGATGC CATGAATTTA TTAGATGGTA TCAAACAATG GCTAAAGTAT 
GTCTCATCGA AGTTTATCGC GGTATCTTTG GAGTCTGACT CCTTAACTAA GACATCGATT 
GCTGCCGTGG AAGATCATAT CATGAAATAC GCAGCTAACA TCGAAGGTCA TGAAAGAAAA 
CAGTTAGCCA AAGTTCCTCG TGAGGCTGTT TTGAATCCAA GATCATCATT TGCACTCTTA 
CGTAGTCAAA AGTTCGATTT GGGTGACCGT GTTGTTTATA TCCAAGATTC TGGTAAGGTA 
CCAATTTTCT CAAAGGGTAC AGTTGTTGGC TATACTACTC TCAGTTCATC ATTATCAATT 
CAGGTCTTAT TTGATCATGA AATCGTGGCC GGTAATAATT TTGGCGGCAG GTTGCGCACG 
AATAGAGGCT TAGGGCTTGA TGCCTCTTTC TTATTGAATA TTACTAACAG GCAGTTCATT 
TATCACTCCA AGGCTTCCAA AAAGGCTTTG GAAAAGAAAA AGCAATCTAA CAATAGGAAC 
AATAATACCA AAACTGCTCA CAAGACTCCT TCAAAGCAAC AATCTGAAGA AAAACTGAGA 
AAAGAAAGGG CACATGATTT ATTGAATTTT ATCAAAAAGG ATACCAATGA AAAGAATTCT 
GAAAGTGTAG ACAACAAGAG CATGGGATCG CAAAAAGATT CCAAACCCGC AAAGAAAGTT 
TTGTTAAAAA GACCAGCTCA GAAAAGCAGT GAAAACGTGC AAGTTGATTT GGCCAATTTT 
GAAAAAGCAC CGCTTGATAA TCCAACTGTT GCTGGATCTA TTTTCAATGC CGTTGCAAAT 
CAATATTCTG ATGGTATAGG CAGTAATTTG AATATCCCAA CTCCACCTCA CCCAATGAAT 
GTGGTTGGGG GTCCTATTCC TGGAGCGAAT GATGTTGCAG ATGTTGGTTT GCCGTACAAT 
ATTCCCCCAG GTTTTATGAC GCATCCTAAT GGTCTTCACC CATTACACCC TCACCAGATG 
CCTTACCCTA ATATGAATGG AATGTCTATT CCGCCACCAG CACCACATGG GTTTGGACAA 
CCGATTTCCT TCCCACCTCC ACCTCCTATG ACAAATGTTT CAGATCAAGG AAGTCGTATT 
GTTGTCAATG AAAAGGAAAG CCAAGATTTG AAAAAATTCA TTAATGGTAA ACAGCACAGC 
AATGGTTCAA CTATTGGGGG AGAAACAAAG AACAGTAGGA AAGGCGAGAT TAAACCTTCT 
TCTGGCACAA ACTCTACTGA ATGTCAATCG CCAAAGTCAC AAAGCAATGC TGCTGACCGT 
GATAATAAAA AAGACGAATC TACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011342.1
CDS	1..4587
Translation	MGIPKFFRYISERWPMILQLIEGTQIPEFDNLYLDMNSILHNCTHGNDDDVTKRLTEEEVFAKICTYIDHLFQTIKPKKIFYMAIDGVAPRAKMNQQRARRFRTAMDAEKALKKAIENGDEIPKGEPFDSNSITPGTEFMAKLTKNLQYFIHDKISNDSKWREVQIIFSGHEVPGEGEHKIMNFIRHLKSQKDFNQNTRHCIYGLDADLIMLGLSTHGPHFALLREEVTFGRRNSEKKSLEHQNFYLLHLSLLREYMELEFKEIADEMQFEYNFERILDDFILVMFVIGNDFLPNLPDLHLNKGAFPVLLQTFKEALLHTDGYINEHGKINLKRLGVWLNYLSQFELLNFEKDDIDVEWFNKQLENISLEGERKRQRVGKKLLVKQQKKLIGSIKPWLMEQLQEKLSPDLPDEEIPTLELPKDLDMKDHLEFLKEFAFDLGLFITHSKSKGSYSLKMDLDSINPDETEEEFQNRVNSIRKTIKKYQNAIIVEDKEELETEKTIYNERFERWKHEYYHDKLKFTTDSEEKVRDLAKDYVEGLQWVLYYYYRGCPSWSWYYPHHYAPRISDLAKGLDQDIEFDLSKPFTPFQQLMAVLPERSKNLIPPAFRPLMYDEQSPIHDFYPAEVQLDKNGKTADWEAVVLISFVDEKRLIEAMQPYLRKLSPEEKTRNQFGKDLIYSFNPQVDNLYKSPLGGIFSDIEHNHCVEKEYITIPLDSSEIRYGLLPNAKLGAEMLAGFPTLLSLPFTSSLEYNETMVFQQPSKQQSMVLQITDIYKTNNVTLEDFSKRHLNKVIYTRWPYLRESKLVSLTDGKTIYEYQESNDKKKFGFITKPAETQDKKLFNSLKNSMLRMYAKQKAVKIGPMEAIATVFPVTGLVRDSDGGYIKTFSPTPDYYPLQLVVESVVNEDERYKERGPIPIEEEFPLNSKVIFLGDYAYGGETTIDGYSSDRRLKITVEKKFLDSEPTIGKERLQMDHQAVKYYPSYIVSKNMHLHPLFLSKITSKFMITDATGKHINVGIPVKFEARHQKVLGYARRNPRGWEYSNLTLNLLKEYRQTFPDFFFRLSKVGNDIPVLEDLFPDTSTKDAMNLLDGIKQWLKYVSSKFIAVSLESDSLTKTSIAAVEDHIMKYAANIEGHERKQLAKVPREAVLNPRSSFALLRSQKFDLGDRVVYIQDSGKVPIFSKGTVVGYTTLSSSLSIQVLFDHEIVAGNNFGGRLRTNRGLGLDASFLLNITNRQFIYHSKASKKALEKKKQSNNRNNNTKTAHKTPSKQQSEEKLRKERAHDLLNFIKKDTNEKNSESVDNKSMGSQKDSKPAKKVLLKRPAQKSSENVQVDLANFEKAPLDNPTVAGSIFNAVANQYSDGIGSNLNIPTPPHPMNVVGGPIPGANDVADVGLPYNIPPGFMTHPNGLHPLHPHQMPYPNMNGMSIPPPAPHGFGQPISFPPPPPMTNVSDQGSRIVVNEKESQDLKKFINGKQHSNGSTIGGETKNSRKGEIKPSSGTNSTECQSPKSQSNAADRDNKKDEST

Target ORF information:

RefSeq Version	NM_001181038.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C chromatin-binding exonuclease XRN1 (XRN1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181038.1

ORF Insert Sequence:

ATGGGTATTC CAAAATTTTT CAGGTACATC TCAGAAAGAT GGCCCATGAT TTTACAGCTT 
ATTGAGGGAA CACAGATTCC TGAGTTTGAT AACTTATACC TGGATATGAA TTCGATTTTA 
CATAATTGTA CGCATGGTAA CGACGATGAT GTAACCAAGC GATTAACTGA AGAAGAGGTT 
TTTGCAAAAA TCTGTACGTA TATCGATCAC CTTTTTCAAA CAATCAAGCC CAAGAAGATT 
TTCTACATGG CTATTGATGG TGTGGCCCCT CGTGCCAAGA TGAATCAACA AAGAGCTCGT 
AGATTCAGAA CCGCTATGGA TGCAGAAAAA GCCTTGAAGA AGGCTATTGA GAATGGTGAC 
GAGATTCCTA AGGGTGAGCC ATTTGATTCG AATTCTATTA CTCCAGGTAC GGAGTTTATG 
GCCAAATTGA CCAAAAACTT ACAATATTTT ATTCACGACA AGATTTCTAA CGATTCCAAA 
TGGAGGGAAG TGCAAATCAT ATTTTCTGGC CATGAAGTTC CAGGTGAAGG TGAACACAAG 
ATCATGAACT TTATAAGGCA TTTAAAATCC CAAAAGGATT TCAACCAGAA TACGAGACAT 
TGTATTTACG GTCTTGACGC AGATTTGATT ATGCTGGGTT TGTCTACTCA TGGGCCACAT 
TTTGCGTTAT TGAGAGAAGA AGTGACATTT GGTAGAAGAA ATAGTGAAAA AAAATCGCTT 
GAACATCAAA ATTTCTACTT ATTACATCTT TCTTTATTAA GAGAATACAT GGAGTTGGAA 
TTCAAAGAAA TTGCCGATGA AATGCAATTT GAATACAATT TTGAACGTAT TTTGGATGAT 
TTTATTCTTG TCATGTTCGT CATTGGTAAT GATTTCTTGC CCAATTTGCC AGATTTGCAC 
CTTAACAAAG GAGCATTTCC CGTTTTGTTA CAAACGTTCA AAGAAGCTCT TTTACATACT 
GATGGCTACA TTAATGAACA TGGTAAAATA AATTTAAAGA GATTAGGTGT CTGGTTAAAT 
TATCTGTCTC AATTTGAGTT ATTAAATTTC GAAAAGGATG ATATAGACGT TGAGTGGTTC 
AACAAGCAAT TAGAGAATAT TTCTTTGGAG GGTGAAAGGA AAAGACAGAG GGTTGGTAAA 
AAATTACTGG TAAAACAACA GAAGAAATTA ATTGGAAGTA TAAAACCATG GTTGATGGAG 
CAATTACAGG AAAAATTATC GCCTGATTTA CCAGATGAAG AAATTCCAAC TTTAGAGTTA 
CCTAAGGACT TAGACATGAA AGATCATTTA GAATTTTTAA AAGAATTCGC TTTTGATTTG 
GGTCTTTTTA TAACGCATTC CAAATCCAAA GGTAGTTATT CGCTAAAAAT GGATCTTGAT 
TCTATTAATC CTGATGAAAC AGAAGAAGAA TTTCAAAATC GTGTTAATTC TATCAGGAAA 
ACAATAAAAA AATATCAAAA TGCTATCATC GTGGAGGACA AAGAAGAATT GGAAACTGAA 
AAAACGATTT ATAATGAAAG GTTTGAACGT TGGAAGCATG AGTATTATCA CGACAAGTTA 
AAATTTACGA CAGACAGTGA AGAAAAAGTG AGAGATCTTG CTAAAGACTA CGTTGAAGGT 
TTACAATGGG TTCTATATTA TTATTATAGA GGATGTCCAT CTTGGTCGTG GTACTATCCG 
CACCATTATG CACCAAGAAT CTCCGACTTA GCCAAGGGTT TAGATCAAGA CATTGAATTT 
GATTTGAGCA AACCATTTAC TCCATTCCAG CAACTAATGG CAGTTTTACC GGAAAGGTCC 
AAAAATCTGA TACCTCCCGC CTTTAGGCCA TTAATGTACG ATGAACAGTC GCCAATCCAC 
GATTTCTATC CCGCTGAGGT TCAACTTGAT AAAAACGGCA AGACAGCTGA TTGGGAAGCT 
GTGGTTTTGA TATCGTTTGT AGATGAAAAA AGGTTGATTG AGGCTATGCA ACCTTATTTG 
CGCAAGTTAT CACCTGAAGA AAAAACGAGA AATCAATTTG GCAAGGACTT GATATATTCC 
TTTAATCCTC AAGTTGATAA CCTTTATAAG AGTCCGTTGG GCGGCATTTT TTCTGATATT 
GAACACAATC ATTGTGTCGA AAAAGAGTAC ATCACCATCC CATTGGACAG CTCCGAGATT 
CGGTATGGTT TATTACCTAA TGCTAAACTC GGTGCCGAAA TGCTGGCGGG TTTCCCCACG 
TTATTGTCTT TACCATTTAC TAGTTCACTG GAGTACAATG AGACAATGGT TTTCCAACAA 
CCTTCTAAAC AACAATCAAT GGTCTTACAA ATAACTGACA TATACAAAAC GAATAATGTT 
ACTTTGGAGG ACTTTTCCAA GAGGCATTTA AACAAAGTGA TTTATACAAG ATGGCCATAT 
TTAAGAGAAT CCAAATTGGT CTCTTTAACG GATGGTAAGA CTATCTATGA ATATCAGGAG 
TCCAATGATA AGAAAAAGTT CGGATTCATA ACGAAGCCTG CGGAAACCCA GGACAAAAAA 
CTTTTCAATA GTTTGAAGAA TTCAATGCTA AGGATGTATG CTAAACAGAA AGCTGTTAAA 
ATAGGACCTA TGGAAGCCAT TGCTACCGTC TTTCCAGTGA CTGGTTTGGT GAGAGACTCT 
GATGGTGGTT ATATTAAGAC CTTTAGCCCT ACCCCAGATT ACTATCCATT GCAACTGGTT 
GTTGAATCTG TTGTCAACGA GGATGAAAGA TATAAAGAAA GAGGACCCAT TCCTATTGAA 
GAGGAATTTC CATTGAATTC AAAAGTTATT TTCTTAGGTG ATTATGCCTA TGGTGGTGAA 
ACTACTATTG ACGGTTACAG CAGTGACCGC AGACTAAAAA TTACTGTAGA AAAGAAGTTT 
TTGGATAGTG AGCCCACCAT CGGCAAAGAA AGGTTACAAA TGGATCATCA AGCCGTTAAA 
TATTATCCGT CTTATATTGT GTCCAAGAAC ATGCACTTAC ACCCCTTGTT TTTGTCTAAG 
ATTACTTCCA AGTTCATGAT TACTGACGCT ACTGGGAAGC ATATCAATGT TGGTATCCCG 
GTTAAGTTCG AAGCTAGACA CCAAAAGGTT TTAGGTTACG CGAGGAGGAA CCCTAGGGGC 
TGGGAATACT CAAATTTAAC TCTAAATTTA CTGAAAGAGT ATAGACAAAC TTTCCCAGAT 
TTTTTTTTCA GGTTGTCCAA GGTAGGTAAT GATATCCCAG TTTTGGAAGA TCTTTTCCCC 
GATACTTCCA CTAAGGATGC CATGAATTTA TTAGATGGTA TCAAACAATG GCTAAAGTAT 
GTCTCATCGA AGTTTATCGC GGTATCTTTG GAGTCTGACT CCTTAACTAA GACATCGATT 
GCTGCCGTGG AAGATCATAT CATGAAATAC GCAGCTAACA TCGAAGGTCA TGAAAGAAAA 
CAGTTAGCCA AAGTTCCTCG TGAGGCTGTT TTGAATCCAA GATCATCATT TGCACTCTTA 
CGTAGTCAAA AGTTCGATTT GGGTGACCGT GTTGTTTATA TCCAAGATTC TGGTAAGGTA 
CCAATTTTCT CAAAGGGTAC AGTTGTTGGC TATACTACTC TCAGTTCATC ATTATCAATT 
CAGGTCTTAT TTGATCATGA AATCGTGGCC GGTAATAATT TTGGCGGCAG GTTGCGCACG 
AATAGAGGCT TAGGGCTTGA TGCCTCTTTC TTATTGAATA TTACTAACAG GCAGTTCATT 
TATCACTCCA AGGCTTCCAA AAAGGCTTTG GAAAAGAAAA AGCAATCTAA CAATAGGAAC 
AATAATACCA AAACTGCTCA CAAGACTCCT TCAAAGCAAC AATCTGAAGA AAAACTGAGA 
AAAGAAAGGG CACATGATTT ATTGAATTTT ATCAAAAAGG ATACCAATGA AAAGAATTCT 
GAAAGTGTAG ACAACAAGAG CATGGGATCG CAAAAAGATT CCAAACCCGC AAAGAAAGTT 
TTGTTAAAAA GACCAGCTCA GAAAAGCAGT GAAAACGTGC AAGTTGATTT GGCCAATTTT 
GAAAAAGCAC CGCTTGATAA TCCAACTGTT GCTGGATCTA TTTTCAATGC CGTTGCAAAT 
CAATATTCTG ATGGTATAGG CAGTAATTTG AATATCCCAA CTCCACCTCA CCCAATGAAT 
GTGGTTGGGG GTCCTATTCC TGGAGCGAAT GATGTTGCAG ATGTTGGTTT GCCGTACAAT 
ATTCCCCCAG GTTTTATGAC GCATCCTAAT GGTCTTCACC CATTACACCC TCACCAGATG 
CCTTACCCTA ATATGAATGG AATGTCTATT CCGCCACCAG CACCACATGG GTTTGGACAA 
CCGATTTCCT TCCCACCTCC ACCTCCTATG ACAAATGTTT CAGATCAAGG AAGTCGTATT 
GTTGTCAATG AAAAGGAAAG CCAAGATTTG AAAAAATTCA TTAATGGTAA ACAGCACAGC 
AATGGTTCAA CTATTGGGGG AGAAACAAAG AACAGTAGGA AAGGCGAGAT TAAACCTTCT 
TCTGGCACAA ACTCTACTGA ATGTCAATCG CCAAAGTCAC AAAGCAATGC TGCTGACCGT 
GATAATAAAA AAGACGAATC TACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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XRN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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