pcm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pcm
Entrez Gene ID	32935
Full Name	pacman
Synonyms	CG3291,DmXrn1,Dmel\CG3291,Pcm,XRN1,XRN1/pcm,Xrn1,Xrn1p
General protein information	Preferred Names pacman Names CG3291 gene product from transcript CG3291-RB CG3291-PA CG3291-PB pcm-PA pcm-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18C7-18C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_078684.4	NP_523408.2	pacman, isoform A
NM_001169325.2	NP_001162796.1	pacman, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	XRN1	NP_061874.3
Mus musculus (house mouse)	Xrn1	NP_036046.2
Rattus norvegicus (Norway rat)	Xrn1	XP_217233.5
Caenorhabditis elegans (roundworm)	xrn-1	NP_496945.3
Schizosaccharomyces pombe (fission yeast)	exo2	NP_593482.1
	XRN1	XP_001112305.1
Saccharomyces cerevisiae (baker's yeast)	XRN1	NP_011342.1
Magnaporthe oryzae (rice blast fungus)	MGG_12403	XP_003715558.1
Canis lupus familiaris (dog)	XRN1	XP_005634594.1
Danio rerio (zebrafish)	xrn1	NP_957327.2
Drosophila melanogaster (fruit fly)	pcm	NP_001162796.1
Eremothecium gossypii	AGOS_ADL046C	NP_984050.1
Neurospora crassa	NCU06678	XP_960925.2
Gallus gallus (chicken)	XRN1	XP_422596.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004430	XP_313715.5
Xenopus tropicalis (tropical clawed frog)	xrn1	XP_002937144.2
Pan troglodytes (chimpanzee)	XRN1	XP_001161640.1
Bos taurus (cattle)	XRN1	NP_001192964.1
Kluyveromyces lactis	KLLA0F22385g	XP_456080.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data suggest that Pacman controls growth of imaginal discs via control of protein synthesis and regulation of autophagy
Title: The 5'-3' exoribonuclease Pacman (Xrn1) regulates expression of the heat shock protein Hsp67Bc and the microRNA miR-277-3p in Drosophila wing imaginal discs.

analysis of coupled 5' nucleotide recognition and processivity in Xrn1-mediated mRNA decay
Title: Coupled 5' nucleotide recognition and processivity in Xrn1-mediated mRNA decay.

Results identify KLP59D as a novel and important regulator of spindle microtubule dynamics and chromosome motility, via Pacman and Flux, in Drosophila somatic cells.
Title: The Drosophila kinesin-13, KLP59D, impacts Pacman- and Flux-based chromosome movement.

Results show that Pacman, the Drosophila homologue of Xrn1, is localized in cytoplasmic particles in Drosophila testis cells and is required for normal spermatogenesis.
Title: The 5'-3' exoribonuclease Pacman is required for normal male fertility and is dynamically localized in cytoplasmic particles in Drosophila testis cells.

Pacman genetically interacts with puckered, a phosphatase that negatively regulates the JNK signalling pathway.
Title: The 5'-3' exoribonuclease pacman is required for epithelial sheet sealing in Drosophila and genetically interacts with the phosphatase puckered.

The following pcm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pcm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02120	NM_078684.4 Latest version!	Drosophila melanogaster pacman (pcm), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa02942	NM_001169325.2 Latest version!	Drosophila melanogaster pacman (pcm), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pcm ( NM_078684.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523408.2 Drosophila melanogaster pacman (pcm), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02120
Clone ID Related Accession (Same CDS sequence)	NM_078684.4
Accession Version	NM_078684.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pacman, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078684.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGTTC CCAAGTTCTT TCGCTACATC AGCGAGCGAT ATCCGTGCCT CAGCGAATTG 
GCGCGGGAGC ACTGCATACC CGAGTTCGAC AATCTGTACT TGGACATGAA CGGCATTGTC 
CACAACTGCT CGCATCCGGA CGACAACAAC ATACACTTTC ACCTGGAGGA GGAGCAGATA 
TTCCAGGAAA TCTTCAACTA CGTAGACAAG CTGTTCTACC TGATCAAGCC GCAGCGCCTC 
TTCTTCCTTT CAGTTGACGG GGTGGCTCCA CGGGCCAAGA TGAACCAGCA GCGCAGCCGG 
CGATTCCGGA CGGCGCGCGA GGCGGAGCAG CAGGAGGCCA AGGCGGCCCA GCGAGGCGAG 
TTGCGAGAGC ACGAGCGCTT CGACAGCAAC TGCATTACGC CGGGCACCGA ATTCATGGTG 
CGTCTGCAGG AGGGACTGCG CGCCTTTCTC AAAACGAAGA TTTCGACGGA TCCGCTGTGG 
CAGCGTTGCA CCGTAATCCT AAGTGGCCAG GAGGCGCCCG GCGAGGGCGA GCACAAGATC 
ATGGACTACA TTCGGTACAT GAAGACGCAG CCGGACTATG ATCCCAATAC ACGACACTGC 
CTTTACGGCC TGGACGCCGA TCTGATCATT CTGGGCCTCT GCACCCACGA GCTGCACTTC 
GTGGTGCTGC GCGAGGAGGT CAAGTTCGGG CGCAACGTCA AGCGCACCTC GGTGGAGGAG 
ACGCGCTTCT TCCTGCTGCA CCTGGGTCTG CTGCGCGAGT ACCTCGAGCT GGAGTTCGAC 
GCCCTGAGAA CAGACGAGCA CAAATTGGAC ATTGCGCAGC TTATCGACGA CTGGGTGCTG 
ATGGGCTTTT TGGTCGGCAA CGACTTCATT CCCCACCTGC CGTGCCTGCA CATTTCATCA 
AATGCACTGC CGCTGCTCTA CCGCACCTAC ATCGGCATTT ACCCGACCCT CGGCGGCAAC 
ATCAACGAGA ACGGCAAGCT CAACCTGCGC CGCTTGCAGA TTTTCATATC GGCCCTCACC 
GAGGTGGAGC TGGATCACTT CAAGGAGCAC GCCGACGACT TGAAGTACAT GAACAACAAA 
TCAGAGGCGT TCGACATGGA CGTCGGCGAG ATCACCGAGT CTCAAAACCT CGACTCCGAT 
CTCGGAGCCC TCATCAACAA GAGCATGCTG CTCTACGATG ACGACAGCGA GGAGGACTGC 
TCCGACGAGA ACGCGGTTCT TCTGAAGGAG TTCCAGAACT ACAAGCGAAA CTTCTACCGC 
AACAAGTTCA AGCGTGATCC GAATGATGAA CTGATCGAGG AACTGTGCCA CCACTACGTC 
AATGCGCTGC AGTGGGTGCT CGACTACTAC TACCGTGGCG TGCAGTCCTG GGACTGGTAC 
TATCCCTTCC ACTACACTCC ATTCATCAGC GACCTCAAGA ACATCGAGCA GGTGGAGATC 
GCCTTCCACA TGGGCACGCC CTTTCTGCCA TTCCAGCAGC TTCTGGCGGT ACTGCCGGCG 
GCCAGTGCAA AGCTTCTCCC TGTCGCCTAC CACGACCTCA TGCTGCTGCC GACCAGCCCG 
CTGGCCGAGT TCTATCCACT GGAGTTCGAG AGCGACCTCA ATGGCAAGAA ACACGATTGG 
GAGGCGGTTG TGCTGATACC CTTCATCGAT GAGGGTCGGC TGCTGGCCGC TATGCTGCCA 
TGCGAGGCTC AGCTGTCGCT GGAGGAGCGC GAACGCAACC GACACGGTCC CATGTATGTG 
TACAAATACA GTACGGTGGC GCAGGGTCCC ATGCCAGCGT ATCCACCGCT GAGGGCTCTT 
CCCGTGCTCT ACTGCACGGA GGTGGCCAAG TGGTCGCACG AAATCGCAGT CAATCTGCCG 
TACAGCGTGT GCATCGAGCT ACCAAATGCG GCGAGAACCG TTTTCTTCCC GGGATTTCCC 
ACCATGCAGC ATCTGCCCTT CGATTTCGAG CTGCGCAACG ACCGCGTCAA GGTGTTCGAA 
CAGGTCAGTC GCAACCAGAA CATTGTGCTA AAACCACGCA AGCGCCAGCT GGAGGACACA 
CTGACGGCGG TGGCCTCCCA GTACCTCGGG AAGGTGATCC ACGTCGGCTG GCCGCATCTC 
GTCAAGGCGA TAGTGGTGCG CGTGGCCACG CGAGATCAGC GCGTCGACAG CGAGGGAATC 
ACGCTAAACG ATTCGCGGCG TTTCGACTCG GAATGCAAGG CACTGCAGGA ACACTTTATC 
AATCGTATGG GAATACAGTT TGCCAACTAT GATGTGCTCG TCTATGTGCG CACCTTTGCC 
GGCAATTCGA CGGAGTTCCG CGACAAGGGG GCATTGATGG TGCGCGACTC GTGGAGCAGC 
TCCGTCACCG GCTATCCAGC CCAGGGCGTG GTGGCCGATC TGACTGTGTG GGAAAGAATG 
CGCAAGAATT TCCTCAACGT GGAGCACTAC TTCCCCGTTG GCAGCACGAT CTTCTTGATC 
ACGGATCCGT ATTACGGCAG CGAGGGAACT GTGCAGGACC CACGGCTGGC CTACACCAAC 
GGCCGGATTC AAGTCAGCAT CATGGTGCGA CCGGAACCGA AAGTAAATGC GGCACGTCAG 
CTGCAGGAAG AGCGGGACCG GGACTATCTG AGCACCTTCC AGGTCTGCAA CTTGCTCCGA 
ATCAGTGGCC GCACCCTGGG ACGCCTAAGT GGCACCGTGT GGGTGGTGCT CGGTCCGCGG 
CGTCAGAAGA TGGAAAACGT GACCAAGCAC AACATTGGCC TGCAGCTCAA GTATCCGCGG 
CAGAACGAGG AGCGTGCCGG ATACTGCTTC CGCACGAACA ACCAGTGGTA CTACTCCAGC 
CTGGCCGTGG ATCTGATGCG CAACTATTGC CAGCGTTACC CAGACGTTAT CGACTTCTTT 
GGCGACTCCA ACGATCGCGC CGAGTTCGTA TTCGAACAGG ACGTGTTCCC CAATGCGGTT 
GGCCATCGCC GCGTCGAGGA ACTGGCCAAC TGGGTGCGCC AGCAGCCGCA CATGAAAGTG 
GAGCGCATCT CTTGCGGCTC CAAGACGGTC TGCCGCGAGA CCATCGAACT GCTGATAGCC 
GCCGTCGACG ACCTGCGCTC ACTGCCCGTC AAGCACGTTA AACTGCAGGT GAAGCCGCAC 
TTGCTCATCA AGCCCAATGT CACACTGCCA GATGTCTACC GATCAAAGCG CCCGGTGCGT 
CTCTTCGATC GCGTCGTAAT CGTACGCACC ATTTACATGG TGCCCGTGGG CACTAAGGGC 
ACCGTGATTG GCATTCATCC CGTTACGGAC CCCAACCCGG TCCGCCTGGA GTGCGTCCAT 
GCTGTCGACA CGTTCTGCAA GGTGCTGTTC GACAGCCCCG TTCCGAACTG CAACAACATC 
CATGGCATCG CCGAAGACCG AGTTTACAAG GTGCCCGAAA TTGCCCTGGT GATCATCAAG 
ACGGATGAGG AAGGAAAGAA GCAGAACGAC TGCGAACTGC CGGTGAGGGA TCCTCAGCCG 
AACCAGGCAC AGGACGAGCC AGTCCGCGCG ACCAGCAGTC GCTATGTGAC CGCCGCAGGG 
TCCACCTCGG TCCCGATCAC GATGAAGACC CAAATTAGCG ACGAATTCGT CAAGACGCGT 
AGCGATCCGA TAGCCCGTAC GGATTCGTAC AAGCCATCGT CGGAGCCAAA ACCGGTGCCG 
GTGCCGGAGC AGATTACCAA CTGGCGGGAA CGCGTCAGTA CGCCAACAAA CAAACCGCAG 
CCTGCGCCAA ATAATTGGCG CATAAATCGG TCTTCATCAC GTCAACAGGG CGGTAGTATT 
TTTGTTGCTC CGCCCACAAA AACTCCTGAT GCAGCTGCAA GCACTGCTTC CACTGCATTC 
ACCGCAGCCT CGTCCGCAAC GCTGACTCCT CTGGATCAGA CCTTGGCGCT CATGTCGGTT 
TTGGGCGTGG GTGAGGACCA GAGCTCGCCG CCACTCCAGG AGGCGGTGCA GCAGCAACGT 
CCGCCACTAC TTCAACAGCA AAGAGCTCCG TTTCCCGGAC AAATGCCCAA TCTACCCAAG 
CCACCGCTCT TCTGGCAGCA AGAGGCGCAG AAGCAGGAGG CGCTGCAGCA GGAGGCGCAG 
CAGCAGGAGG CGCAGAAGAA GCAGCAGCAG GCGCATGCGC AAATGGAGCC TGAACGTATC 
AATAGCCAGC ATTTCTACAG AAGCGGCCAG ACAGGAGCGG CGTTGAATCA GCCGCCATTG 
GGTGCTCCAT CAAAAAGGCA GTGGCATGAG TGGGTGCACC CGCGCATGCA GCACGCCAAC 
GCATTCCACG CTGGCGTCAA CAATGGATAT CAGATGCGTC CCAAAAAGAA TATAGCTGCG 
CAGAGCACCT TTAACAACAA CGTGCACATG CACCTCCAGC AACCATATTA CCCGAATCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCCACTC CAGCTGACGG AGATCAACAA TGCGCCACCG 
CGATACTCAA CGATCCAAGA TTTTGTGCCC ATACAGGCGT ACCGTCCCAA GAAATTAAAT 
CGCGTCCAAC CAGCCGGTCG TCAGGACGTC GATGCCACCA AGAATCCTAG TCGTTCGCCT 
GTGTTGCAGC AACCGACGAA CGAGACTATA GACACAAAGG CCAGTTCTAG TCTTCCGGTA 
CAATCAGCAG GCGAACAGGT GATTGGACTG ATGCAAACGC TTGAAATTAA GCCGGCTGCG 
TCGCAGTCCG AATCTGATGG GGTCTCAACA GGCAGTGCGA ACGCGCCCAC AGCAACGACA 
TCATCCCAGG CGGTCAACCG AAGGAAGCAT CGAGTGCCTC GCATCGGAGC CAAGTTCGAC 
TTGGAATACA TACTGCCCGA CTCCCCGCAT CCAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523408.2
CDS	199..5037
Translation	MGVPKFFRYISERYPCLSELAREHCIPEFDNLYLDMNGIVHNCSHPDDNNIHFHLEEEQIFQEIFNYVDKLFYLIKPQRLFFLSVDGVAPRAKMNQQRSRRFRTAREAEQQEAKAAQRGELREHERFDSNCITPGTEFMVRLQEGLRAFLKTKISTDPLWQRCTVILSGQEAPGEGEHKIMDYIRYMKTQPDYDPNTRHCLYGLDADLIILGLCTHELHFVVLREEVKFGRNVKRTSVEETRFFLLHLGLLREYLELEFDALRTDEHKLDIAQLIDDWVLMGFLVGNDFIPHLPCLHISSNALPLLYRTYIGIYPTLGGNINENGKLNLRRLQIFISALTEVELDHFKEHADDLKYMNNKSEAFDMDVGEITESQNLDSDLGALINKSMLLYDDDSEEDCSDENAVLLKEFQNYKRNFYRNKFKRDPNDELIEELCHHYVNALQWVLDYYYRGVQSWDWYYPFHYTPFISDLKNIEQVEIAFHMGTPFLPFQQLLAVLPAASAKLLPVAYHDLMLLPTSPLAEFYPLEFESDLNGKKHDWEAVVLIPFIDEGRLLAAMLPCEAQLSLEERERNRHGPMYVYKYSTVAQGPMPAYPPLRALPVLYCTEVAKWSHEIAVNLPYSVCIELPNAARTVFFPGFPTMQHLPFDFELRNDRVKVFEQVSRNQNIVLKPRKRQLEDTLTAVASQYLGKVIHVGWPHLVKAIVVRVATRDQRVDSEGITLNDSRRFDSECKALQEHFINRMGIQFANYDVLVYVRTFAGNSTEFRDKGALMVRDSWSSSVTGYPAQGVVADLTVWERMRKNFLNVEHYFPVGSTIFLITDPYYGSEGTVQDPRLAYTNGRIQVSIMVRPEPKVNAARQLQEERDRDYLSTFQVCNLLRISGRTLGRLSGTVWVVLGPRRQKMENVTKHNIGLQLKYPRQNEERAGYCFRTNNQWYYSSLAVDLMRNYCQRYPDVIDFFGDSNDRAEFVFEQDVFPNAVGHRRVEELANWVRQQPHMKVERISCGSKTVCRETIELLIAAVDDLRSLPVKHVKLQVKPHLLIKPNVTLPDVYRSKRPVRLFDRVVIVRTIYMVPVGTKGTVIGIHPVTDPNPVRLECVHAVDTFCKVLFDSPVPNCNNIHGIAEDRVYKVPEIALVIIKTDEEGKKQNDCELPVRDPQPNQAQDEPVRATSSRYVTAAGSTSVPITMKTQISDEFVKTRSDPIARTDSYKPSSEPKPVPVPEQITNWRERVSTPTNKPQPAPNNWRINRSSSRQQGGSIFVAPPTKTPDAAASTASTAFTAASSATLTPLDQTLALMSVLGVGEDQSSPPLQEAVQQQRPPLLQQQRAPFPGQMPNLPKPPLFWQQEAQKQEALQQEAQQQEAQKKQQQAHAQMEPERINSQHFYRSGQTGAALNQPPLGAPSKRQWHEWVHPRMQHANAFHAGVNNGYQMRPKKNIAAQSTFNNNVHMHLQQPYYPNQQQQQQQQQPLQLTEINNAPPRYSTIQDFVPIQAYRPKKLNRVQPAGRQDVDATKNPSRSPVLQQPTNETIDTKASSSLPVQSAGEQVIGLMQTLEIKPAASQSESDGVSTGSANAPTATTSSQAVNRRKHRVPRIGAKFDLEYILPDSPHPT

Target ORF information:

RefSeq Version	NM_078684.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pacman (pcm), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078684.4

ORF Insert Sequence:

ATGGGCGTTC CCAAGTTCTT TCGCTACATC AGCGAGCGAT ATCCGTGCCT CAGCGAATTG 
GCGCGGGAGC ACTGCATACC CGAGTTCGAC AATCTGTACT TGGACATGAA CGGCATTGTC 
CACAACTGCT CGCATCCGGA CGACAACAAC ATACACTTTC ACCTGGAGGA GGAGCAGATA 
TTCCAGGAAA TCTTCAACTA CGTAGACAAG CTGTTCTACC TGATCAAGCC GCAGCGCCTC 
TTCTTCCTTT CAGTTGACGG GGTGGCTCCA CGGGCCAAGA TGAACCAGCA GCGCAGCCGG 
CGATTCCGGA CGGCGCGCGA GGCGGAGCAG CAGGAGGCCA AGGCGGCCCA GCGAGGCGAG 
TTGCGAGAGC ACGAGCGCTT CGACAGCAAC TGCATTACGC CGGGCACCGA ATTCATGGTG 
CGTCTGCAGG AGGGACTGCG CGCCTTTCTC AAAACGAAGA TTTCGACGGA TCCGCTGTGG 
CAGCGTTGCA CCGTAATCCT AAGTGGCCAG GAGGCGCCCG GCGAGGGCGA GCACAAGATC 
ATGGACTACA TTCGGTACAT GAAGACGCAG CCGGACTATG ATCCCAATAC ACGACACTGC 
CTTTACGGCC TGGACGCCGA TCTGATCATT CTGGGCCTCT GCACCCACGA GCTGCACTTC 
GTGGTGCTGC GCGAGGAGGT CAAGTTCGGG CGCAACGTCA AGCGCACCTC GGTGGAGGAG 
ACGCGCTTCT TCCTGCTGCA CCTGGGTCTG CTGCGCGAGT ACCTCGAGCT GGAGTTCGAC 
GCCCTGAGAA CAGACGAGCA CAAATTGGAC ATTGCGCAGC TTATCGACGA CTGGGTGCTG 
ATGGGCTTTT TGGTCGGCAA CGACTTCATT CCCCACCTGC CGTGCCTGCA CATTTCATCA 
AATGCACTGC CGCTGCTCTA CCGCACCTAC ATCGGCATTT ACCCGACCCT CGGCGGCAAC 
ATCAACGAGA ACGGCAAGCT CAACCTGCGC CGCTTGCAGA TTTTCATATC GGCCCTCACC 
GAGGTGGAGC TGGATCACTT CAAGGAGCAC GCCGACGACT TGAAGTACAT GAACAACAAA 
TCAGAGGCGT TCGACATGGA CGTCGGCGAG ATCACCGAGT CTCAAAACCT CGACTCCGAT 
CTCGGAGCCC TCATCAACAA GAGCATGCTG CTCTACGATG ACGACAGCGA GGAGGACTGC 
TCCGACGAGA ACGCGGTTCT TCTGAAGGAG TTCCAGAACT ACAAGCGAAA CTTCTACCGC 
AACAAGTTCA AGCGTGATCC GAATGATGAA CTGATCGAGG AACTGTGCCA CCACTACGTC 
AATGCGCTGC AGTGGGTGCT CGACTACTAC TACCGTGGCG TGCAGTCCTG GGACTGGTAC 
TATCCCTTCC ACTACACTCC ATTCATCAGC GACCTCAAGA ACATCGAGCA GGTGGAGATC 
GCCTTCCACA TGGGCACGCC CTTTCTGCCA TTCCAGCAGC TTCTGGCGGT ACTGCCGGCG 
GCCAGTGCAA AGCTTCTCCC TGTCGCCTAC CACGACCTCA TGCTGCTGCC GACCAGCCCG 
CTGGCCGAGT TCTATCCACT GGAGTTCGAG AGCGACCTCA ATGGCAAGAA ACACGATTGG 
GAGGCGGTTG TGCTGATACC CTTCATCGAT GAGGGTCGGC TGCTGGCCGC TATGCTGCCA 
TGCGAGGCTC AGCTGTCGCT GGAGGAGCGC GAACGCAACC GACACGGTCC CATGTATGTG 
TACAAATACA GTACGGTGGC GCAGGGTCCC ATGCCAGCGT ATCCACCGCT GAGGGCTCTT 
CCCGTGCTCT ACTGCACGGA GGTGGCCAAG TGGTCGCACG AAATCGCAGT CAATCTGCCG 
TACAGCGTGT GCATCGAGCT ACCAAATGCG GCGAGAACCG TTTTCTTCCC GGGATTTCCC 
ACCATGCAGC ATCTGCCCTT CGATTTCGAG CTGCGCAACG ACCGCGTCAA GGTGTTCGAA 
CAGGTCAGTC GCAACCAGAA CATTGTGCTA AAACCACGCA AGCGCCAGCT GGAGGACACA 
CTGACGGCGG TGGCCTCCCA GTACCTCGGG AAGGTGATCC ACGTCGGCTG GCCGCATCTC 
GTCAAGGCGA TAGTGGTGCG CGTGGCCACG CGAGATCAGC GCGTCGACAG CGAGGGAATC 
ACGCTAAACG ATTCGCGGCG TTTCGACTCG GAATGCAAGG CACTGCAGGA ACACTTTATC 
AATCGTATGG GAATACAGTT TGCCAACTAT GATGTGCTCG TCTATGTGCG CACCTTTGCC 
GGCAATTCGA CGGAGTTCCG CGACAAGGGG GCATTGATGG TGCGCGACTC GTGGAGCAGC 
TCCGTCACCG GCTATCCAGC CCAGGGCGTG GTGGCCGATC TGACTGTGTG GGAAAGAATG 
CGCAAGAATT TCCTCAACGT GGAGCACTAC TTCCCCGTTG GCAGCACGAT CTTCTTGATC 
ACGGATCCGT ATTACGGCAG CGAGGGAACT GTGCAGGACC CACGGCTGGC CTACACCAAC 
GGCCGGATTC AAGTCAGCAT CATGGTGCGA CCGGAACCGA AAGTAAATGC GGCACGTCAG 
CTGCAGGAAG AGCGGGACCG GGACTATCTG AGCACCTTCC AGGTCTGCAA CTTGCTCCGA 
ATCAGTGGCC GCACCCTGGG ACGCCTAAGT GGCACCGTGT GGGTGGTGCT CGGTCCGCGG 
CGTCAGAAGA TGGAAAACGT GACCAAGCAC AACATTGGCC TGCAGCTCAA GTATCCGCGG 
CAGAACGAGG AGCGTGCCGG ATACTGCTTC CGCACGAACA ACCAGTGGTA CTACTCCAGC 
CTGGCCGTGG ATCTGATGCG CAACTATTGC CAGCGTTACC CAGACGTTAT CGACTTCTTT 
GGCGACTCCA ACGATCGCGC CGAGTTCGTA TTCGAACAGG ACGTGTTCCC CAATGCGGTT 
GGCCATCGCC GCGTCGAGGA ACTGGCCAAC TGGGTGCGCC AGCAGCCGCA CATGAAAGTG 
GAGCGCATCT CTTGCGGCTC CAAGACGGTC TGCCGCGAGA CCATCGAACT GCTGATAGCC 
GCCGTCGACG ACCTGCGCTC ACTGCCCGTC AAGCACGTTA AACTGCAGGT GAAGCCGCAC 
TTGCTCATCA AGCCCAATGT CACACTGCCA GATGTCTACC GATCAAAGCG CCCGGTGCGT 
CTCTTCGATC GCGTCGTAAT CGTACGCACC ATTTACATGG TGCCCGTGGG CACTAAGGGC 
ACCGTGATTG GCATTCATCC CGTTACGGAC CCCAACCCGG TCCGCCTGGA GTGCGTCCAT 
GCTGTCGACA CGTTCTGCAA GGTGCTGTTC GACAGCCCCG TTCCGAACTG CAACAACATC 
CATGGCATCG CCGAAGACCG AGTTTACAAG GTGCCCGAAA TTGCCCTGGT GATCATCAAG 
ACGGATGAGG AAGGAAAGAA GCAGAACGAC TGCGAACTGC CGGTGAGGGA TCCTCAGCCG 
AACCAGGCAC AGGACGAGCC AGTCCGCGCG ACCAGCAGTC GCTATGTGAC CGCCGCAGGG 
TCCACCTCGG TCCCGATCAC GATGAAGACC CAAATTAGCG ACGAATTCGT CAAGACGCGT 
AGCGATCCGA TAGCCCGTAC GGATTCGTAC AAGCCATCGT CGGAGCCAAA ACCGGTGCCG 
GTGCCGGAGC AGATTACCAA CTGGCGGGAA CGCGTCAGTA CGCCAACAAA CAAACCGCAG 
CCTGCGCCAA ATAATTGGCG CATAAATCGG TCTTCATCAC GTCAACAGGG CGGTAGTATT 
TTTGTTGCTC CGCCCACAAA AACTCCTGAT GCAGCTGCAA GCACTGCTTC CACTGCATTC 
ACCGCAGCCT CGTCCGCAAC GCTGACTCCT CTGGATCAGA CCTTGGCGCT CATGTCGGTT 
TTGGGCGTGG GTGAGGACCA GAGCTCGCCG CCACTCCAGG AGGCGGTGCA GCAGCAACGT 
CCGCCACTAC TTCAACAGCA AAGAGCTCCG TTTCCCGGAC AAATGCCCAA TCTACCCAAG 
CCACCGCTCT TCTGGCAGCA AGAGGCGCAG AAGCAGGAGG CGCTGCAGCA GGAGGCGCAG 
CAGCAGGAGG CGCAGAAGAA GCAGCAGCAG GCGCATGCGC AAATGGAGCC TGAACGTATC 
AATAGCCAGC ATTTCTACAG AAGCGGCCAG ACAGGAGCGG CGTTGAATCA GCCGCCATTG 
GGTGCTCCAT CAAAAAGGCA GTGGCATGAG TGGGTGCACC CGCGCATGCA GCACGCCAAC 
GCATTCCACG CTGGCGTCAA CAATGGATAT CAGATGCGTC CCAAAAAGAA TATAGCTGCG 
CAGAGCACCT TTAACAACAA CGTGCACATG CACCTCCAGC AACCATATTA CCCGAATCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCCACTC CAGCTGACGG AGATCAACAA TGCGCCACCG 
CGATACTCAA CGATCCAAGA TTTTGTGCCC ATACAGGCGT ACCGTCCCAA GAAATTAAAT 
CGCGTCCAAC CAGCCGGTCG TCAGGACGTC GATGCCACCA AGAATCCTAG TCGTTCGCCT 
GTGTTGCAGC AACCGACGAA CGAGACTATA GACACAAAGG CCAGTTCTAG TCTTCCGGTA 
CAATCAGCAG GCGAACAGGT GATTGGACTG ATGCAAACGC TTGAAATTAA GCCGGCTGCG 
TCGCAGTCCG AATCTGATGG GGTCTCAACA GGCAGTGCGA ACGCGCCCAC AGCAACGACA 
TCATCCCAGG CGGTCAACCG AAGGAAGCAT CGAGTGCCTC GCATCGGAGC CAAGTTCGAC 
TTGGAATACA TACTGCCCGA CTCCCCGCAT CCAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcm ( NM_001169325.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162796.1 Drosophila melanogaster pacman (pcm), transcript variant B, mRNA.

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CloneID	OFa02942
Clone ID Related Accession (Same CDS sequence)	NM_001169325.2
Accession Version	NM_001169325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pacman, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169325.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGTTC CCAAGTTCTT TCGCTACATC AGCGAGCGAT ATCCGTGCCT CAGCGAATTG 
GCGCGGGAGC ACTGCATACC CGAGTTCGAC AATCTGTACT TGGACATGAA CGGCATTGTC 
CACAACTGCT CGCATCCGGA CGACAACAAC ATACACTTTC ACCTGGAGGA GGAGCAGATA 
TTCCAGGAAA TCTTCAACTA CGTAGACAAG CTGTTCTACC TGATCAAGCC GCAGCGCCTC 
TTCTTCCTTT CAGTTGACGG GGTGGCTCCA CGGGCCAAGA TGAACCAGCA GCGCAGCCGG 
CGATTCCGGA CGGCGCGCGA GGCGGAGCAG CAGGAGGCCA AGGCGGCCCA GCGAGGCGAG 
TTGCGAGAGC ACGAGCGCTT CGACAGCAAC TGCATTACGC CGGGCACCGA ATTCATGGTG 
CGTCTGCAGG AGGGACTGCG CGCCTTTCTC AAAACGAAGA TTTCGACGGA TCCGCTGTGG 
CAGCGTTGCA CCGTAATCCT AAGTGGCCAG GAGGCGCCCG GCGAGGGCGA GCACAAGATC 
ATGGACTACA TTCGGTACAT GAAGACGCAG CCGGACTATG ATCCCAATAC ACGACACTGC 
CTTTACGGCC TGGACGCCGA TCTGATCATT CTGGGCCTCT GCACCCACGA GCTGCACTTC 
GTGGTGCTGC GCGAGGAGGT CAAGTTCGGG CGCAACGTCA AGCGCACCTC GGTGGAGGAG 
ACGCGCTTCT TCCTGCTGCA CCTGGGTCTG CTGCGCGAGT ACCTCGAGCT GGAGTTCGAC 
GCCCTGAGAA CAGACGAGCA CAAATTGGAC ATTGCGCAGC TTATCGACGA CTGGGTGCTG 
ATGGGCTTTT TGGTCGGCAA CGACTTCATT CCCCACCTGC CGTGCCTGCA CATTTCATCA 
AATGCACTGC CGCTGCTCTA CCGCACCTAC ATCGGCATTT ACCCGACCCT CGGCGGCAAC 
ATCAACGAGA ACGGCAAGCT CAACCTGCGC CGCTTGCAGA TTTTCATATC GGCCCTCACC 
GAGGTGGAGC TGGATCACTT CAAGGAGCAC GCCGACGACT TGAAGTACAT GAACAACAAA 
TCAGAGGCGT TCGACATGGA CGTCGGCGAG ATCACCGAGT CTCAAAACCT CGACTCCGAT 
CTCGGAGCCC TCATCAACAA GAGCATGCTG CTCTACGATG ACGACAGCGA GGAGGACTGC 
TCCGACGAGA ACGCGGTTCT TCTGAAGGAG TTCCAGAACT ACAAGCGAAA CTTCTACCGC 
AACAAGTTCA AGCGTGATCC GAATGATGAA CTGATCGAGG AACTGTGCCA CCACTACGTC 
AATGCGCTGC AGTGGGTGCT CGACTACTAC TACCGTGGCG TGCAGTCCTG GGACTGGTAC 
TATCCCTTCC ACTACACTCC ATTCATCAGC GACCTCAAGA ACATCGAGCA GGTGGAGATC 
GCCTTCCACA TGGGCACGCC CTTTCTGCCA TTCCAGCAGC TTCTGGCGGT ACTGCCGGCG 
GCCAGTGCAA AGCTTCTCCC TGTCGCCTAC CACGACCTCA TGCTGCTGCC GACCAGCCCG 
CTGGCCGAGT TCTATCCACT GGAGTTCGAG AGCGACCTCA ATGGCAAGAA ACACGATTGG 
GAGGCGGTTG TGCTGATACC CTTCATCGAT GAGGGTCGGC TGCTGGCCGC TATGCTGCCA 
TGCGAGGCTC AGCTGTCGCT GGAGGAGCGC GAACGCAACC GACACGGTCC CATGTATGTG 
TACAAATACA GTACGGTGGC GCAGGGTCCC ATGCCAGCGT ATCCACCGCT GAGGGCTCTT 
CCCGTGCTCT ACTGCACGGA GGTGGCCAAG TGGTCGCACG AAATCGCAGT CAATCTGCCG 
TACAGCGTGT GCATCGAGCT ACCAAATGCG GCGAGAACCG TTTTCTTCCC GGGATTTCCC 
ACCATGCAGC ATCTGCCCTT CGATTTCGAG CTGCGCAACG ACCGCGTCAA GGTGTTCGAA 
CAGGTCAGTC GCAACCAGAA CATTGTGCTA AAACCACGCA AGCGCCAGCT GGAGGACACA 
CTGACGGCGG TGGCCTCCCA GTACCTCGGG AAGGTGATCC ACGTCGGCTG GCCGCATCTC 
GTCAAGGCGA TAGTGGTGCG CGTGGCCACG CGAGATCAGC GCGTCGACAG CGAGGGAATC 
ACGCTAAACG ATTCGCGGCG TTTCGACTCG GAATGCAAGG CACTGCAGGA ACACTTTATC 
AATCGTATGG GAATACAGTT TGCCAACTAT GATGTGCTCG TCTATGTGCG CACCTTTGCC 
GGCAATTCGA CGGAGTTCCG CGACAAGGGG GCATTGATGG TGCGCGACTC GTGGAGCAGC 
TCCGTCACCG GCTATCCAGC CCAGGGCGTG GTGGCCGATC TGACTGTGTG GGAAAGAATG 
CGCAAGAATT TCCTCAACGT GGAGCACTAC TTCCCCGTTG GCAGCACGAT CTTCTTGATC 
ACGGATCCGT ATTACGGCAG CGAGGGAACT GTGCAGGACC CACGGCTGGC CTACACCAAC 
GGCCGGATTC AAGTCAGCAT CATGGTGCGA CCGGAACCGA AAGTAAATGC GGCACGTCAG 
CTGCAGGAAG AGCGGGACCG GGACTATCTG AGCACCTTCC AGGTCTGCAA CTTGCTCCGA 
ATCAGTGGCC GCACCCTGGG ACGCCTAAGT GGCACCGTGT GGGTGGTGCT CGGTCCGCGG 
CGTCAGAAGA TGGAAAACGT GACCAAGCAC AACATTGGCC TGCAGCTCAA GTATCCGCGG 
CAGAACGAGG AGCGTGCCGG ATACTGCTTC CGCACGAACA ACCAGTGGTA CTACTCCAGC 
CTGGCCGTGG ATCTGATGCG CAACTATTGC CAGCGTTACC CAGACGTTAT CGACTTCTTT 
GGCGACTCCA ACGATCGCGC CGAGTTCGTA TTCGAACAGG ACGTGTTCCC CAATGCGGTT 
GGCCATCGCC GCGTCGAGGA ACTGGCCAAC TGGGTGCGCC AGCAGCCGCA CATGAAAGTG 
GAGCGCATCT CTTGCGGCTC CAAGACGGTC TGCCGCGAGA CCATCGAACT GCTGATAGCC 
GCCGTCGACG ACCTGCGCTC ACTGCCCGTC AAGCACGTTA AACTGCAGGT GAAGCCGCAC 
TTGCTCATCA AGCCCAATGT CACACTGCCA GATGTCTACC GATCAAAGCG CCCGGTGCGT 
CTCTTCGATC GCGTCGTAAT CGTACGCACC ATTTACATGG TGCCCGTGGG CACTAAGGGC 
ACCGTGATTG GCATTCATCC CGTTACGGAC CCCAACCCGG TCCGCCTGGA GTGCGTCCAT 
GCTGTCGACA CGTTCTGCAA GGTGCTGTTC GACAGCCCCG TTCCGAACTG CAACAACATC 
CATGGCATCG CCGAAGACCG AGTTTACAAG GTGCCCGAAA TTGCCCTGGT GATCATCAAG 
ACGGATGAGG AAGGAAAGAA GCAGAACGAC TGCGAACTGC CGGTGAGGGA TCCTCAGCCG 
AACCAGGCAC AGGACGAGCC AGTCCGCGCG ACCAGCAGTC GCTATGTGAC CGCCGCAGGG 
TCCACCTCGG TCCCGATCAC GATGAAGACC CAAATTAGCG ACGAATTCGT CAAGACGCGT 
AGCGATCCGA TAGCCCGTAC GGATTCGTAC AAGCCATCGT CGGAGCCAAA ACCGGTGCCG 
GTGCCGGAGC AGATTACCAA CTGGCGGGAA CGCGTCAGTA CGCCAACAAA CAAACCGCAG 
CCTGCGCCAA ATAATTGGCG CATAAATCGG TCTTCATCAC GTCAACAGGG CGGTAGTATT 
TTTGTTGCTC CGCCCACAAA AACTCCTGAT GCAGCTGCAA GCACTGCTTC CACTGCATTC 
ACCGCAGCCT CGTCCGCAAC GCTGACTCCT CTGGATCAGA CCTTGGCGCT CATGTCGGTT 
TTGGGCGTGG GTGAGGACCA GAGCTCGCCG CCACTCCAGG AGGCGGTGCA GCAGCAACGT 
CCGCCACTAC TTCAACAGCA AAGAGCTCCG TTTCCCGGAC AAATGCCCAA TCTACCCAAG 
CCACCGCTCT TCTGGCAGCA AGAGGCGCAG AAGCAGGAGG CGCTGCAGCA GGAGGCGCAG 
CAGCAGGAGG CGCAGAAGAA GCAGCAGCAG GCGCATGCGC AAATGGAGCC TGAACGTATC 
AATAGCCAGC ATTTCTACAG AAGCGGCCAG ACAGGAGCGG CGTTGAATCA GCCGCCATTG 
GGTGCTCCAT CAAAAAGGCA GTGGCATGAG TGGGTGCACC CGCGCATGCA GCACGCCAAC 
GCATTCCACG CTGGCGTCAA CAATGGATAT CAGATGCGTC CCAAAAAGAA TATAGCTGCG 
CAGAGCACCT TTAACAACAA CGTGCACATG CACCTCCAGC AACCATATTA CCCGAATCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCCACTC CAGCTGACGG AGATCAACAA TGCGCCACCG 
CGATACTCAA CGATCCAAGA TTTTGTGCCC ATACAGGCGT ACCGTCCCAA GAAATTAAAT 
CGCGTCCAAC CAGCCGGTCG TCAGGACGTC GATGCCACCA AGAATCCTAG TCGTTCGCCT 
GTGTTGCAGC AACCGACGAA CGAGACTATA GACACAAAGG CCAGTTCTAG TCTTCCGGTA 
CAATCAGCAG GCGAACAGGT GATTGGACTG ATGCAAACGC TTGAAATTAA GCAGCCGGCT 
GCGTCGCAGT CCGAATCTGA TGGGGTCTCA ACAGGCAGTG CGAACGCGCC CACAGCAACG 
ACATCATCCC AGGCGGTCAA CCGAAGGAAG CATCGAGTGC CTCGCATCGG AGCCAAGTTC 
GACTTGGAAT ACATACTGCC CGACTCCCCG CATCCAACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162796.1
CDS	199..5040
Translation	MGVPKFFRYISERYPCLSELAREHCIPEFDNLYLDMNGIVHNCSHPDDNNIHFHLEEEQIFQEIFNYVDKLFYLIKPQRLFFLSVDGVAPRAKMNQQRSRRFRTAREAEQQEAKAAQRGELREHERFDSNCITPGTEFMVRLQEGLRAFLKTKISTDPLWQRCTVILSGQEAPGEGEHKIMDYIRYMKTQPDYDPNTRHCLYGLDADLIILGLCTHELHFVVLREEVKFGRNVKRTSVEETRFFLLHLGLLREYLELEFDALRTDEHKLDIAQLIDDWVLMGFLVGNDFIPHLPCLHISSNALPLLYRTYIGIYPTLGGNINENGKLNLRRLQIFISALTEVELDHFKEHADDLKYMNNKSEAFDMDVGEITESQNLDSDLGALINKSMLLYDDDSEEDCSDENAVLLKEFQNYKRNFYRNKFKRDPNDELIEELCHHYVNALQWVLDYYYRGVQSWDWYYPFHYTPFISDLKNIEQVEIAFHMGTPFLPFQQLLAVLPAASAKLLPVAYHDLMLLPTSPLAEFYPLEFESDLNGKKHDWEAVVLIPFIDEGRLLAAMLPCEAQLSLEERERNRHGPMYVYKYSTVAQGPMPAYPPLRALPVLYCTEVAKWSHEIAVNLPYSVCIELPNAARTVFFPGFPTMQHLPFDFELRNDRVKVFEQVSRNQNIVLKPRKRQLEDTLTAVASQYLGKVIHVGWPHLVKAIVVRVATRDQRVDSEGITLNDSRRFDSECKALQEHFINRMGIQFANYDVLVYVRTFAGNSTEFRDKGALMVRDSWSSSVTGYPAQGVVADLTVWERMRKNFLNVEHYFPVGSTIFLITDPYYGSEGTVQDPRLAYTNGRIQVSIMVRPEPKVNAARQLQEERDRDYLSTFQVCNLLRISGRTLGRLSGTVWVVLGPRRQKMENVTKHNIGLQLKYPRQNEERAGYCFRTNNQWYYSSLAVDLMRNYCQRYPDVIDFFGDSNDRAEFVFEQDVFPNAVGHRRVEELANWVRQQPHMKVERISCGSKTVCRETIELLIAAVDDLRSLPVKHVKLQVKPHLLIKPNVTLPDVYRSKRPVRLFDRVVIVRTIYMVPVGTKGTVIGIHPVTDPNPVRLECVHAVDTFCKVLFDSPVPNCNNIHGIAEDRVYKVPEIALVIIKTDEEGKKQNDCELPVRDPQPNQAQDEPVRATSSRYVTAAGSTSVPITMKTQISDEFVKTRSDPIARTDSYKPSSEPKPVPVPEQITNWRERVSTPTNKPQPAPNNWRINRSSSRQQGGSIFVAPPTKTPDAAASTASTAFTAASSATLTPLDQTLALMSVLGVGEDQSSPPLQEAVQQQRPPLLQQQRAPFPGQMPNLPKPPLFWQQEAQKQEALQQEAQQQEAQKKQQQAHAQMEPERINSQHFYRSGQTGAALNQPPLGAPSKRQWHEWVHPRMQHANAFHAGVNNGYQMRPKKNIAAQSTFNNNVHMHLQQPYYPNQQQQQQQQQPLQLTEINNAPPRYSTIQDFVPIQAYRPKKLNRVQPAGRQDVDATKNPSRSPVLQQPTNETIDTKASSSLPVQSAGEQVIGLMQTLEIKQPAASQSESDGVSTGSANAPTATTSSQAVNRRKHRVPRIGAKFDLEYILPDSPHPT

Target ORF information:

RefSeq Version	NM_001169325.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pacman (pcm), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169325.2

ORF Insert Sequence:

ATGGGCGTTC CCAAGTTCTT TCGCTACATC AGCGAGCGAT ATCCGTGCCT CAGCGAATTG 
GCGCGGGAGC ACTGCATACC CGAGTTCGAC AATCTGTACT TGGACATGAA CGGCATTGTC 
CACAACTGCT CGCATCCGGA CGACAACAAC ATACACTTTC ACCTGGAGGA GGAGCAGATA 
TTCCAGGAAA TCTTCAACTA CGTAGACAAG CTGTTCTACC TGATCAAGCC GCAGCGCCTC 
TTCTTCCTTT CAGTTGACGG GGTGGCTCCA CGGGCCAAGA TGAACCAGCA GCGCAGCCGG 
CGATTCCGGA CGGCGCGCGA GGCGGAGCAG CAGGAGGCCA AGGCGGCCCA GCGAGGCGAG 
TTGCGAGAGC ACGAGCGCTT CGACAGCAAC TGCATTACGC CGGGCACCGA ATTCATGGTG 
CGTCTGCAGG AGGGACTGCG CGCCTTTCTC AAAACGAAGA TTTCGACGGA TCCGCTGTGG 
CAGCGTTGCA CCGTAATCCT AAGTGGCCAG GAGGCGCCCG GCGAGGGCGA GCACAAGATC 
ATGGACTACA TTCGGTACAT GAAGACGCAG CCGGACTATG ATCCCAATAC ACGACACTGC 
CTTTACGGCC TGGACGCCGA TCTGATCATT CTGGGCCTCT GCACCCACGA GCTGCACTTC 
GTGGTGCTGC GCGAGGAGGT CAAGTTCGGG CGCAACGTCA AGCGCACCTC GGTGGAGGAG 
ACGCGCTTCT TCCTGCTGCA CCTGGGTCTG CTGCGCGAGT ACCTCGAGCT GGAGTTCGAC 
GCCCTGAGAA CAGACGAGCA CAAATTGGAC ATTGCGCAGC TTATCGACGA CTGGGTGCTG 
ATGGGCTTTT TGGTCGGCAA CGACTTCATT CCCCACCTGC CGTGCCTGCA CATTTCATCA 
AATGCACTGC CGCTGCTCTA CCGCACCTAC ATCGGCATTT ACCCGACCCT CGGCGGCAAC 
ATCAACGAGA ACGGCAAGCT CAACCTGCGC CGCTTGCAGA TTTTCATATC GGCCCTCACC 
GAGGTGGAGC TGGATCACTT CAAGGAGCAC GCCGACGACT TGAAGTACAT GAACAACAAA 
TCAGAGGCGT TCGACATGGA CGTCGGCGAG ATCACCGAGT CTCAAAACCT CGACTCCGAT 
CTCGGAGCCC TCATCAACAA GAGCATGCTG CTCTACGATG ACGACAGCGA GGAGGACTGC 
TCCGACGAGA ACGCGGTTCT TCTGAAGGAG TTCCAGAACT ACAAGCGAAA CTTCTACCGC 
AACAAGTTCA AGCGTGATCC GAATGATGAA CTGATCGAGG AACTGTGCCA CCACTACGTC 
AATGCGCTGC AGTGGGTGCT CGACTACTAC TACCGTGGCG TGCAGTCCTG GGACTGGTAC 
TATCCCTTCC ACTACACTCC ATTCATCAGC GACCTCAAGA ACATCGAGCA GGTGGAGATC 
GCCTTCCACA TGGGCACGCC CTTTCTGCCA TTCCAGCAGC TTCTGGCGGT ACTGCCGGCG 
GCCAGTGCAA AGCTTCTCCC TGTCGCCTAC CACGACCTCA TGCTGCTGCC GACCAGCCCG 
CTGGCCGAGT TCTATCCACT GGAGTTCGAG AGCGACCTCA ATGGCAAGAA ACACGATTGG 
GAGGCGGTTG TGCTGATACC CTTCATCGAT GAGGGTCGGC TGCTGGCCGC TATGCTGCCA 
TGCGAGGCTC AGCTGTCGCT GGAGGAGCGC GAACGCAACC GACACGGTCC CATGTATGTG 
TACAAATACA GTACGGTGGC GCAGGGTCCC ATGCCAGCGT ATCCACCGCT GAGGGCTCTT 
CCCGTGCTCT ACTGCACGGA GGTGGCCAAG TGGTCGCACG AAATCGCAGT CAATCTGCCG 
TACAGCGTGT GCATCGAGCT ACCAAATGCG GCGAGAACCG TTTTCTTCCC GGGATTTCCC 
ACCATGCAGC ATCTGCCCTT CGATTTCGAG CTGCGCAACG ACCGCGTCAA GGTGTTCGAA 
CAGGTCAGTC GCAACCAGAA CATTGTGCTA AAACCACGCA AGCGCCAGCT GGAGGACACA 
CTGACGGCGG TGGCCTCCCA GTACCTCGGG AAGGTGATCC ACGTCGGCTG GCCGCATCTC 
GTCAAGGCGA TAGTGGTGCG CGTGGCCACG CGAGATCAGC GCGTCGACAG CGAGGGAATC 
ACGCTAAACG ATTCGCGGCG TTTCGACTCG GAATGCAAGG CACTGCAGGA ACACTTTATC 
AATCGTATGG GAATACAGTT TGCCAACTAT GATGTGCTCG TCTATGTGCG CACCTTTGCC 
GGCAATTCGA CGGAGTTCCG CGACAAGGGG GCATTGATGG TGCGCGACTC GTGGAGCAGC 
TCCGTCACCG GCTATCCAGC CCAGGGCGTG GTGGCCGATC TGACTGTGTG GGAAAGAATG 
CGCAAGAATT TCCTCAACGT GGAGCACTAC TTCCCCGTTG GCAGCACGAT CTTCTTGATC 
ACGGATCCGT ATTACGGCAG CGAGGGAACT GTGCAGGACC CACGGCTGGC CTACACCAAC 
GGCCGGATTC AAGTCAGCAT CATGGTGCGA CCGGAACCGA AAGTAAATGC GGCACGTCAG 
CTGCAGGAAG AGCGGGACCG GGACTATCTG AGCACCTTCC AGGTCTGCAA CTTGCTCCGA 
ATCAGTGGCC GCACCCTGGG ACGCCTAAGT GGCACCGTGT GGGTGGTGCT CGGTCCGCGG 
CGTCAGAAGA TGGAAAACGT GACCAAGCAC AACATTGGCC TGCAGCTCAA GTATCCGCGG 
CAGAACGAGG AGCGTGCCGG ATACTGCTTC CGCACGAACA ACCAGTGGTA CTACTCCAGC 
CTGGCCGTGG ATCTGATGCG CAACTATTGC CAGCGTTACC CAGACGTTAT CGACTTCTTT 
GGCGACTCCA ACGATCGCGC CGAGTTCGTA TTCGAACAGG ACGTGTTCCC CAATGCGGTT 
GGCCATCGCC GCGTCGAGGA ACTGGCCAAC TGGGTGCGCC AGCAGCCGCA CATGAAAGTG 
GAGCGCATCT CTTGCGGCTC CAAGACGGTC TGCCGCGAGA CCATCGAACT GCTGATAGCC 
GCCGTCGACG ACCTGCGCTC ACTGCCCGTC AAGCACGTTA AACTGCAGGT GAAGCCGCAC 
TTGCTCATCA AGCCCAATGT CACACTGCCA GATGTCTACC GATCAAAGCG CCCGGTGCGT 
CTCTTCGATC GCGTCGTAAT CGTACGCACC ATTTACATGG TGCCCGTGGG CACTAAGGGC 
ACCGTGATTG GCATTCATCC CGTTACGGAC CCCAACCCGG TCCGCCTGGA GTGCGTCCAT 
GCTGTCGACA CGTTCTGCAA GGTGCTGTTC GACAGCCCCG TTCCGAACTG CAACAACATC 
CATGGCATCG CCGAAGACCG AGTTTACAAG GTGCCCGAAA TTGCCCTGGT GATCATCAAG 
ACGGATGAGG AAGGAAAGAA GCAGAACGAC TGCGAACTGC CGGTGAGGGA TCCTCAGCCG 
AACCAGGCAC AGGACGAGCC AGTCCGCGCG ACCAGCAGTC GCTATGTGAC CGCCGCAGGG 
TCCACCTCGG TCCCGATCAC GATGAAGACC CAAATTAGCG ACGAATTCGT CAAGACGCGT 
AGCGATCCGA TAGCCCGTAC GGATTCGTAC AAGCCATCGT CGGAGCCAAA ACCGGTGCCG 
GTGCCGGAGC AGATTACCAA CTGGCGGGAA CGCGTCAGTA CGCCAACAAA CAAACCGCAG 
CCTGCGCCAA ATAATTGGCG CATAAATCGG TCTTCATCAC GTCAACAGGG CGGTAGTATT 
TTTGTTGCTC CGCCCACAAA AACTCCTGAT GCAGCTGCAA GCACTGCTTC CACTGCATTC 
ACCGCAGCCT CGTCCGCAAC GCTGACTCCT CTGGATCAGA CCTTGGCGCT CATGTCGGTT 
TTGGGCGTGG GTGAGGACCA GAGCTCGCCG CCACTCCAGG AGGCGGTGCA GCAGCAACGT 
CCGCCACTAC TTCAACAGCA AAGAGCTCCG TTTCCCGGAC AAATGCCCAA TCTACCCAAG 
CCACCGCTCT TCTGGCAGCA AGAGGCGCAG AAGCAGGAGG CGCTGCAGCA GGAGGCGCAG 
CAGCAGGAGG CGCAGAAGAA GCAGCAGCAG GCGCATGCGC AAATGGAGCC TGAACGTATC 
AATAGCCAGC ATTTCTACAG AAGCGGCCAG ACAGGAGCGG CGTTGAATCA GCCGCCATTG 
GGTGCTCCAT CAAAAAGGCA GTGGCATGAG TGGGTGCACC CGCGCATGCA GCACGCCAAC 
GCATTCCACG CTGGCGTCAA CAATGGATAT CAGATGCGTC CCAAAAAGAA TATAGCTGCG 
CAGAGCACCT TTAACAACAA CGTGCACATG CACCTCCAGC AACCATATTA CCCGAATCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCCACTC CAGCTGACGG AGATCAACAA TGCGCCACCG 
CGATACTCAA CGATCCAAGA TTTTGTGCCC ATACAGGCGT ACCGTCCCAA GAAATTAAAT 
CGCGTCCAAC CAGCCGGTCG TCAGGACGTC GATGCCACCA AGAATCCTAG TCGTTCGCCT 
GTGTTGCAGC AACCGACGAA CGAGACTATA GACACAAAGG CCAGTTCTAG TCTTCCGGTA 
CAATCAGCAG GCGAACAGGT GATTGGACTG ATGCAAACGC TTGAAATTAA GCAGCCGGCT 
GCGTCGCAGT CCGAATCTGA TGGGGTCTCA ACAGGCAGTG CGAACGCGCC CACAGCAACG 
ACATCATCCC AGGCGGTCAA CCGAAGGAAG CATCGAGTGC CTCGCATCGG AGCCAAGTTC 
GACTTGGAAT ACATACTGCC CGACTCCCCG CATCCAACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pcm cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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