pol1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	pol1
Entrez Gene ID	2543629
Full Name	DNA polymerase alpha catalytic subunit
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019610.2	NP_594186.1	DNA polymerase alpha catalytic subunit

Pathways from BioSystems

Homologies

	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Neurospora crassa	NCU07870	XP_963131.2
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fen-1 and Swi-7 proteins act together in the S-phase alkylation damage response in S. pombe.
Title: DNA polymerase ? (swi7) and the flap endonuclease Fen1 (rad2) act together in the S-phase alkylation damage response in S. pombe.

GINS functions in a pathway that involves Cdc45 and is necessary for DNA polymerase epsilon chromatin binding, but a separate pathway sets up the chromatin association of DNA polymerase alpha.
Title: GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.

The following pol1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pol1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102733	NM_001019610.2 Latest version!	Schizosaccharomyces pombe DNA polymerase alpha catalytic subunit (pol1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pol1 ( NM_001019610.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594186.1 Schizosaccharomyces pombe DNA polymerase alpha catalytic subunit (pol1), mRNA.

Price & Availability↑

CloneID	OSc102733
Clone ID Related Accession (Same CDS sequence)	NM_001019610.2
Accession Version	NM_001019610.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	DNA polymerase alpha catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019610.1.

ATGAGAAAGA GAAACGCGGG GATAAAGCCG TCTCAAAGAT TTGCTGAGTT ACGGGCTCTT 
CGTCAAGCCG GAAAAACAAG GGCTAGCACA TACGAAAGCA AAGAAAATGA AGAATTATAT 
GACAATGTTT CAGAGGAAGA ATATCGCAAG ATTGTTCGAC AACGACTGGA TGAGGATGAC 
TTTGTCGTAG ACGATAACGG GGCTGGTTAT GTGGATAATG GATATGATGA GTGGGATCAA 
AGTCATTACA GTGACGAGGA TGATGAAAAT GAAAAAGGAT CGTCTGGTCG AAAAAGAAAG 
AAATCGAAGA ACGTTCTAGC TGAACCTAAT CAACAAATTG GCGCGTTTTT TAAAGCAAAT 
GCTATCCAAC CTGCTTCACG TATCGCAAAT CGAAAAAATA ATGAGAAGGA AGATGAATTC 
ATGGCTGAAA TTCTTGGCTC AATCGACCAA GATATTCCCG AACGCCTCTC GACAAAAAAG 
GGAAATCGTT CTCATACTAC ATCAAATGCC AAACGTCGCT CTCAGAAAGC TGCTTCCTCA 
ATAAGTACAA CTAACGTTGT TTCTTCTGAT CCCAAAAAAT ACATCCCAGA AACTGTACCT 
TCAACTCCTC AACCTGCTAG CAGTCTTCCT ATTCCCAGCT CCCCACCTGC TGCACTCGAG 
ACAGAAGAAA ATGTCGACAG TCAACCAATG GAATTAGACG AACCAGAACT CCCCGTTGGA 
TCTGATGCAC CCATTATTGA CGTTGAAGAA TCTATTGCCA TGAAGCCATT GATGAGTCAT 
GATCAGGTCG ATGACACCGC ATCGCTTTCA AAAGCTACTA TTTCTCAGCA ACCTCTATCA 
CCAATGACAC CTTTATCTTC GGAACTTGAC GTCTCTGCTT TTTCTGAAAT TAATTCTAAG 
ATAAAGAATG TAGATGTTCC TGCTTCGTCC TATTCATCTC CAATTTCAAA AGTATCACCA 
TCTGATGTTA CTGAAGAGGA TGGTTCTTTG TTTTTTTTTT GGATGGATTA TACCGAGATG 
TACGGCTCAT TGTGTCTATT TGGCAAAGTA TATGATAAAG CTACTAAACA GTATGTTTCG 
TGCTTTCTCA AGGTCGATGG GATAATGCGT TCTTTATACT TCCTTCCCCG CCCATCATCA 
TCCTCAGTTT CTGAAGATTC TATTGCAGCG CAAACTAAAG ATGTTTACGA CGAAGTAGCC 
AATTTGTTAT CTAAAAGGGG TGTCAAGGAA TGGAAATCAA GAGTCTCCAA GTATAAATAT 
GCTTTCGAAT TAGAAGATGT TCCTCGGACC GCTGATTACT TAGAGGTAAT TTATTCCTAT 
TCTTATCCTG CTCTGCCTAC TGATTTGACT GGTTCTTCTT TCTCACACGT TTTTGGAACT 
AATACCGCCT TATTCGAGCA GTTTGTCTTA AGTAGAAGAG TTATGGGTCC TTGTTGGCTG 
AAAATACAAC AACCAAATTT TGATGCTGTA AAAAATGCTA GTTGGTGCCG GGTCGAAATA 
GGATGTTCGT CACCTCAGAA CATTTCTGTT TCTTTCGAAA AAAATGAAAT TACTTCGAAA 
ACGCCTCCAA TGACGGTGAT GAGTCTTGCA TTTAGAACGC TGATTAACAA AGAGCAAAAC 
AAGCAAGAAG TCGTTATGAT TAGTGCTAGG ATTTTTGAAA ATGTTGACAT TGAAAAAGGT 
TTGCCTGCAA ATGACATGCC CTCGTATTCC TTTTCGTTGA TTCGTCCTTT GAAGCAAATC 
TTTCCAAATG GTTTTGAAAA GCTAGCTCGC CAGCACAAAT CTTCCATCTT TTGTGAGAGG 
TCTGAAGTTT CGTTGCTTAA TAATTTCTTA AATAAAGTTC GTACATACGA TCCTGATGTT 
TATTTTGGGC ATGATTTTGA AATGTGCTAC TCAGTATTGT TATCTAGACT GAAAGAACGA 
AAAATTCATA ATTGGAGTAG TATTGGTCGA CTTCGAAGAA GCGAATGGCC ACGTTCGTTT 
AATAGAAGCT CTCAACAGTT CGTAGAAAAA CAGATTATTG CTGGAAGGCT TATGTGTGAT 
TTATCTAACG ACTTTGGTCG ATCTATGATT AAAGCCCAAT CTTGGAGTTT ATCTGAAATA 
GTACTTAAGG AGCTTGACAT TAAACGTCAA GACATTAATC AAGAGAAGGC ATTGCAATCA 
TGGACCGATA CGGCTCACGG CTTATTGGAT TATTTAGTTC ACTGTGAAAT TGACACATTC 
TTTATTGCTG CCGTCGCATT CAAAATTCAG ATGCTTCAGC TATCAAAGAA TTTAACAAAC 
ATTGCTGGTA ACTCTTGGGC TAGAACATTA ACAGGTACCA GAGCGGAAAG AAACGAATAC 
ATTCTTCTTC ACGAATTTAA AAAAAATGGA TATATTGTCC CGGATAAACA ACAAAGTATT 
AGAAGACATG CTGAAGCTTT TGGTGCAGAA GACGGTCTGC AAGAAGAATC TCTTGGCAAG 
AAAAAAGATA AATACAAAGG TGGATTGGTT TTTGAGCCTC AAAAAGGACT TTATGAAACT 
TGTATTTTGG TGATGGACTT CAATAGTTTA TATCCAAGTA TTATCCAAGA ATACAATATT 
TGTTTTACTA CTGTTGACAG ATCTCCTTCT AACTCTGATT CCGACGATCA AATTCCCGAT 
ACTCCATCTG CCTCGGCCAA TCAAGGTATA TTTCCTCGAT TAATTGCAAA TCTTGTGGAA 
CGTCGACGCC AAATTAAAGG ACTATTAAAG GATAACTCTG CCACTCCCAC ACAAAGGCTG 
CAATGGGATA TCCAACAACA AGCCCTGAAG TTAACTGCAA ACTCCATGTA TGGCTGCCTG 
GGATATACGA AGTCTCGATT CTATGCACGT CCGTTGGCTG TTCTGATTAC TTATAAGGGT 
AGGGAAGCCT TGATGAATAC TAAAGAACTT GCGGATCAAA TGGGACTTCA AGTAATTTAT 
GGCGATACCG ATTCAGTTAT GCTCAATACG AACGTAACAG ACAAGAACCA TGCTCTTCGA 
ATTGGTAATG AATTTAAGGA AAAGGTAAAT GAACGTTATA GTAAACTAGA AATTGATATC 
GATAACGTTT ATCAGCGCAT GCTTCTGCAT GCTAAGAAAA AGTACGCGGC TTTACAGCTT 
GATTCTCAAG GGAAGCCCAA TTTAGATGTA AAGGGCTTGG ATATGAAACG TCGTGAGTTT 
TGTACTTTAG CTAAGGAGGC TAGTAAATTT TGTTTGGATC AAATTTTATC TGGTGAATTA 
ACGGAAACCG TAATTGAAAA TATTCATTCT TATTTAATGG ATTTTTCCGA AAAAATGCGT 
AATGGGAAGT TTCCGGCTAA CAAATTTATA ATATTCAATC GTTTGGGAAA AAATCCAGAA 
GATTATCCAA ATGGAAAGAC GATGCCATTC GTGCAGGTGG CTCTTAAAAA GAAAGCTAGA 
GGAGAGAATG TTCGTGTAGG CGATGTTATT CCTTTCATTA TTGCTGGTAG TGACGCTGAC 
GGACACCCTG CCGACCGAGC TTATAGTCCG CAAGAAATAA TGAATACAAA TTCCACATTA 
GTTATTGACT ACAATTATTA TCTTTCGCAT CAAATTCTCC CTCCTATCGA GCGTGTTATA 
GCACCGATTG AAGGAACAAA CCGTGCTCGC TTAGCTGAAT GCTTAGGTTT GGATGCTCGA 
AAATACTACT CACATGAGAC TTCTGAATCA TCTGCGTTTC AGCGGTATGA GTCTACGCTC 
ACTGATGACC AGTGTTTTAT TAACGTTTCC CCATTACTAT TGAAATGTCC ATCCTGTAAT 
GCTACATCAT TTTCCCTACG ATCTGTAAAG TCCTTGAAGG AAACGTTGTA TGCGAATACA 
GTAGAATGTG ATTGTGGATA TGAGTATTCG GACTTTACTA TTATTCTTCA ATTCTCATCT 
CAACTCAGAG ATTTTATTAA TCTTTATTAT GAAGGAATTT TAGTATGTGA TGATTCTTCT 
TGTGGTAATC GAACACGACA AATGAGTGTG TATGGAAAGC GTTGTTGTAA CAAATCATGT 
CGTGGAAGCG TTCATTTTGA ATATAATGAT GAACAATTAT ATAACCAGAT TAAGTTCCTG 
CTAAAAGCTG TTCAAACAAC CACCGGGGCT ACCAGAAATG GAATCATTAG ATGCAATGCC 
ATCAACAAAA ATATCTCTCG AATAATGAAC AAAAATGCGC GTGAATTTGT AGATATGGGA 
CTGATATTTT CATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594186.1
CDS	129..4346
Translation	MRKRNAGIKPSQRFAELRALRQAGKTRASTYESKENEELYDNVSEEEYRKIVRQRLDEDDFVVDDNGAGYVDNGYDEWDQSHYSDEDDENEKGSSGRKRKKSKNVLAEPNQQIGAFFKANAIQPASRIANRKNNEKEDEFMAEILGSIDQDIPERLSTKKGNRSHTTSNAKRRSQKAASSISTTNVVSSDPKKYIPETVPSTPQPASSLPIPSSPPAALETEENVDSQPMELDEPELPVGSDAPIIDVEESIAMKPLMSHDQVDDTASLSKATISQQPLSPMTPLSSELDVSAFSEINSKIKNVDVPASSYSSPISKVSPSDVTEEDGSLFFFWMDYTEMYGSLCLFGKVYDKATKQYVSCFLKVDGIMRSLYFLPRPSSSSVSEDSIAAQTKDVYDEVANLLSKRGVKEWKSRVSKYKYAFELEDVPRTADYLEVIYSYSYPALPTDLTGSSFSHVFGTNTALFEQFVLSRRVMGPCWLKIQQPNFDAVKNASWCRVEIGCSSPQNISVSFEKNEITSKTPPMTVMSLAFRTLINKEQNKQEVVMISARIFENVDIEKGLPANDMPSYSFSLIRPLKQIFPNGFEKLARQHKSSIFCERSEVSLLNNFLNKVRTYDPDVYFGHDFEMCYSVLLSRLKERKIHNWSSIGRLRRSEWPRSFNRSSQQFVEKQIIAGRLMCDLSNDFGRSMIKAQSWSLSEIVLKELDIKRQDINQEKALQSWTDTAHGLLDYLVHCEIDTFFIAAVAFKIQMLQLSKNLTNIAGNSWARTLTGTRAERNEYILLHEFKKNGYIVPDKQQSIRRHAEAFGAEDGLQEESLGKKKDKYKGGLVFEPQKGLYETCILVMDFNSLYPSIIQEYNICFTTVDRSPSNSDSDDQIPDTPSASANQGIFPRLIANLVERRRQIKGLLKDNSATPTQRLQWDIQQQALKLTANSMYGCLGYTKSRFYARPLAVLITYKGREALMNTKELADQMGLQVIYGDTDSVMLNTNVTDKNHALRIGNEFKEKVNERYSKLEIDIDNVYQRMLLHAKKKYAALQLDSQGKPNLDVKGLDMKRREFCTLAKEASKFCLDQILSGELTETVIENIHSYLMDFSEKMRNGKFPANKFIIFNRLGKNPEDYPNGKTMPFVQVALKKKARGENVRVGDVIPFIIAGSDADGHPADRAYSPQEIMNTNSTLVIDYNYYLSHQILPPIERVIAPIEGTNRARLAECLGLDARKYYSHETSESSAFQRYESTLTDDQCFINVSPLLLKCPSCNATSFSLRSVKSLKETLYANTVECDCGYEYSDFTIILQFSSQLRDFINLYYEGILVCDDSSCGNRTRQMSVYGKRCCNKSCRGSVHFEYNDEQLYNQIKFLLKAVQTTTGATRNGIIRCNAINKNISRIMNKNAREFVDMGLIFSS

Target ORF information:

RefSeq Version	NM_001019610.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe DNA polymerase alpha catalytic subunit (pol1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019610.2

ORF Insert Sequence:

ATGAGAAAGA GAAACGCGGG GATAAAGCCG TCTCAAAGAT TTGCTGAGTT ACGGGCTCTT 
CGTCAAGCCG GAAAAACAAG GGCTAGCACA TACGAAAGCA AAGAAAATGA AGAATTATAT 
GACAATGTTT CAGAGGAAGA ATATCGCAAG ATTGTTCGAC AACGACTGGA TGAGGATGAC 
TTTGTCGTAG ACGATAACGG GGCTGGTTAT GTGGATAATG GATATGATGA GTGGGATCAA 
AGTCATTACA GTGACGAGGA TGATGAAAAT GAAAAAGGAT CGTCTGGTCG AAAAAGAAAG 
AAATCGAAGA ACGTTCTAGC TGAACCTAAT CAACAAATTG GCGCGTTTTT TAAAGCAAAT 
GCTATCCAAC CTGCTTCACG TATCGCAAAT CGAAAAAATA ATGAGAAGGA AGATGAATTC 
ATGGCTGAAA TTCTTGGCTC AATCGACCAA GATATTCCCG AACGCCTCTC GACAAAAAAG 
GGAAATCGTT CTCATACTAC ATCAAATGCC AAACGTCGCT CTCAGAAAGC TGCTTCCTCA 
ATAAGTACAA CTAACGTTGT TTCTTCTGAT CCCAAAAAAT ACATCCCAGA AACTGTACCT 
TCAACTCCTC AACCTGCTAG CAGTCTTCCT ATTCCCAGCT CCCCACCTGC TGCACTCGAG 
ACAGAAGAAA ATGTCGACAG TCAACCAATG GAATTAGACG AACCAGAACT CCCCGTTGGA 
TCTGATGCAC CCATTATTGA CGTTGAAGAA TCTATTGCCA TGAAGCCATT GATGAGTCAT 
GATCAGGTCG ATGACACCGC ATCGCTTTCA AAAGCTACTA TTTCTCAGCA ACCTCTATCA 
CCAATGACAC CTTTATCTTC GGAACTTGAC GTCTCTGCTT TTTCTGAAAT TAATTCTAAG 
ATAAAGAATG TAGATGTTCC TGCTTCGTCC TATTCATCTC CAATTTCAAA AGTATCACCA 
TCTGATGTTA CTGAAGAGGA TGGTTCTTTG TTTTTTTTTT GGATGGATTA TACCGAGATG 
TACGGCTCAT TGTGTCTATT TGGCAAAGTA TATGATAAAG CTACTAAACA GTATGTTTCG 
TGCTTTCTCA AGGTCGATGG GATAATGCGT TCTTTATACT TCCTTCCCCG CCCATCATCA 
TCCTCAGTTT CTGAAGATTC TATTGCAGCG CAAACTAAAG ATGTTTACGA CGAAGTAGCC 
AATTTGTTAT CTAAAAGGGG TGTCAAGGAA TGGAAATCAA GAGTCTCCAA GTATAAATAT 
GCTTTCGAAT TAGAAGATGT TCCTCGGACC GCTGATTACT TAGAGGTAAT TTATTCCTAT 
TCTTATCCTG CTCTGCCTAC TGATTTGACT GGTTCTTCTT TCTCACACGT TTTTGGAACT 
AATACCGCCT TATTCGAGCA GTTTGTCTTA AGTAGAAGAG TTATGGGTCC TTGTTGGCTG 
AAAATACAAC AACCAAATTT TGATGCTGTA AAAAATGCTA GTTGGTGCCG GGTCGAAATA 
GGATGTTCGT CACCTCAGAA CATTTCTGTT TCTTTCGAAA AAAATGAAAT TACTTCGAAA 
ACGCCTCCAA TGACGGTGAT GAGTCTTGCA TTTAGAACGC TGATTAACAA AGAGCAAAAC 
AAGCAAGAAG TCGTTATGAT TAGTGCTAGG ATTTTTGAAA ATGTTGACAT TGAAAAAGGT 
TTGCCTGCAA ATGACATGCC CTCGTATTCC TTTTCGTTGA TTCGTCCTTT GAAGCAAATC 
TTTCCAAATG GTTTTGAAAA GCTAGCTCGC CAGCACAAAT CTTCCATCTT TTGTGAGAGG 
TCTGAAGTTT CGTTGCTTAA TAATTTCTTA AATAAAGTTC GTACATACGA TCCTGATGTT 
TATTTTGGGC ATGATTTTGA AATGTGCTAC TCAGTATTGT TATCTAGACT GAAAGAACGA 
AAAATTCATA ATTGGAGTAG TATTGGTCGA CTTCGAAGAA GCGAATGGCC ACGTTCGTTT 
AATAGAAGCT CTCAACAGTT CGTAGAAAAA CAGATTATTG CTGGAAGGCT TATGTGTGAT 
TTATCTAACG ACTTTGGTCG ATCTATGATT AAAGCCCAAT CTTGGAGTTT ATCTGAAATA 
GTACTTAAGG AGCTTGACAT TAAACGTCAA GACATTAATC AAGAGAAGGC ATTGCAATCA 
TGGACCGATA CGGCTCACGG CTTATTGGAT TATTTAGTTC ACTGTGAAAT TGACACATTC 
TTTATTGCTG CCGTCGCATT CAAAATTCAG ATGCTTCAGC TATCAAAGAA TTTAACAAAC 
ATTGCTGGTA ACTCTTGGGC TAGAACATTA ACAGGTACCA GAGCGGAAAG AAACGAATAC 
ATTCTTCTTC ACGAATTTAA AAAAAATGGA TATATTGTCC CGGATAAACA ACAAAGTATT 
AGAAGACATG CTGAAGCTTT TGGTGCAGAA GACGGTCTGC AAGAAGAATC TCTTGGCAAG 
AAAAAAGATA AATACAAAGG TGGATTGGTT TTTGAGCCTC AAAAAGGACT TTATGAAACT 
TGTATTTTGG TGATGGACTT CAATAGTTTA TATCCAAGTA TTATCCAAGA ATACAATATT 
TGTTTTACTA CTGTTGACAG ATCTCCTTCT AACTCTGATT CCGACGATCA AATTCCCGAT 
ACTCCATCTG CCTCGGCCAA TCAAGGTATA TTTCCTCGAT TAATTGCAAA TCTTGTGGAA 
CGTCGACGCC AAATTAAAGG ACTATTAAAG GATAACTCTG CCACTCCCAC ACAAAGGCTG 
CAATGGGATA TCCAACAACA AGCCCTGAAG TTAACTGCAA ACTCCATGTA TGGCTGCCTG 
GGATATACGA AGTCTCGATT CTATGCACGT CCGTTGGCTG TTCTGATTAC TTATAAGGGT 
AGGGAAGCCT TGATGAATAC TAAAGAACTT GCGGATCAAA TGGGACTTCA AGTAATTTAT 
GGCGATACCG ATTCAGTTAT GCTCAATACG AACGTAACAG ACAAGAACCA TGCTCTTCGA 
ATTGGTAATG AATTTAAGGA AAAGGTAAAT GAACGTTATA GTAAACTAGA AATTGATATC 
GATAACGTTT ATCAGCGCAT GCTTCTGCAT GCTAAGAAAA AGTACGCGGC TTTACAGCTT 
GATTCTCAAG GGAAGCCCAA TTTAGATGTA AAGGGCTTGG ATATGAAACG TCGTGAGTTT 
TGTACTTTAG CTAAGGAGGC TAGTAAATTT TGTTTGGATC AAATTTTATC TGGTGAATTA 
ACGGAAACCG TAATTGAAAA TATTCATTCT TATTTAATGG ATTTTTCCGA AAAAATGCGT 
AATGGGAAGT TTCCGGCTAA CAAATTTATA ATATTCAATC GTTTGGGAAA AAATCCAGAA 
GATTATCCAA ATGGAAAGAC GATGCCATTC GTGCAGGTGG CTCTTAAAAA GAAAGCTAGA 
GGAGAGAATG TTCGTGTAGG CGATGTTATT CCTTTCATTA TTGCTGGTAG TGACGCTGAC 
GGACACCCTG CCGACCGAGC TTATAGTCCG CAAGAAATAA TGAATACAAA TTCCACATTA 
GTTATTGACT ACAATTATTA TCTTTCGCAT CAAATTCTCC CTCCTATCGA GCGTGTTATA 
GCACCGATTG AAGGAACAAA CCGTGCTCGC TTAGCTGAAT GCTTAGGTTT GGATGCTCGA 
AAATACTACT CACATGAGAC TTCTGAATCA TCTGCGTTTC AGCGGTATGA GTCTACGCTC 
ACTGATGACC AGTGTTTTAT TAACGTTTCC CCATTACTAT TGAAATGTCC ATCCTGTAAT 
GCTACATCAT TTTCCCTACG ATCTGTAAAG TCCTTGAAGG AAACGTTGTA TGCGAATACA 
GTAGAATGTG ATTGTGGATA TGAGTATTCG GACTTTACTA TTATTCTTCA ATTCTCATCT 
CAACTCAGAG ATTTTATTAA TCTTTATTAT GAAGGAATTT TAGTATGTGA TGATTCTTCT 
TGTGGTAATC GAACACGACA AATGAGTGTG TATGGAAAGC GTTGTTGTAA CAAATCATGT 
CGTGGAAGCG TTCATTTTGA ATATAATGAT GAACAATTAT ATAACCAGAT TAAGTTCCTG 
CTAAAAGCTG TTCAAACAAC CACCGGGGCT ACCAGAAATG GAATCATTAG ATGCAATGCC 
ATCAACAAAA ATATCTCTCG AATAATGAAC AAAAATGCGC GTGAATTTGT AGATATGGGA 
CTGATATTTT CATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
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pol1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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