pub1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following pub1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pub1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc102592 NM_001019819.2
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Schizosaccharomyces pombe HECT-type ubiquitin-protein ligase E3 Pub1 (pub1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc102592
    Clone ID Related Accession (Same CDS sequence) NM_001019819.2
    Accession Version NM_001019819.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product HECT-type ubiquitin-protein ligase E3 Pub1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019819.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCAAACT CAGCTCAATC TCGTCGAATT CGAGTAACAA TTGTTGCTGC TGATGGCCTT 
    TACAAACGAG ATGTGTTTCG TTTTCCTGAC CCGTTTGCGG TACTAACAGT GGATGGTGAG
    CAAACACATA CCACAACTGC TATCAAAAAG ACGTTAAACC CTTATTGGAA TGAGACTTTT
    GAAGTTAATG TTACAGATAA TAGCACCATT GCGATTCAGG TGTTCGATCA GAAAAAATTT
    AAGAAAAAAG GCCAAGGCTT TCTAGGAGTG ATAAATCTTC GTGTTGGAGA TGTGCTCGAT
    CTCGCCATTG GAGGTGATGA AATGTTGACC CGCGATTTGA AAAAGTCAAA TGAGAATACA
    GTAGTTCATG GCAAGATAAT CATAAACTTA TCAACGACTG CGCAGTCAAC CTTACAGGTT
    CCATCCAGTG CAGCATCAGG TGCACGTACC CAACGTACTA GCATTACCAA TGACCCGCAA
    AGCTCACAAA GTAGCAGTGT GTCTCGTAAC CCTGCTTCCT CTCGTGCTGG TTCACCTACC
    CGCGACAACG CACCTGCTGC TTCCCCTGCT AGTTCAGAAC CTCGCACATT TAGTTCATTT
    GAAGATCAAT ATGGGCGCCT TCCTCCTGGA TGGGAAAGAC GTACCGATAA TCTTGGCCGA
    ACTTATTATG TAGATCATAA TACAAGAAGC ACAACGTGGA TTCGCCCCAA CCTAAGTTCT
    GTTGCCGGAG CAGCCGCAGC CGAATTGCAT AGTAGTGCAT CGTCTGCGAA TGTTACCGAA
    GGTGTTCAAC CTTCCTCTAG CAATGCAGCT CGTCGTACAG AAGCTAGTGT TTTGACCTCT
    AACGCTACTA CTGCTGGTTC AGGAGAGCTT CCACCGGGAT GGGAGCAAAG GTATACACCA
    GAGGGTCGAC CCTACTTTGT GGATCATAAT ACGCGAACTA CTACTTGGGT GGATCCGCGC
    CGTCAACAGT ACATTCGTTC CTATGGCGGT CCTAATAATG CTACTATTCA GCAACAACCT
    GTCTCTCAAC TTGGTCCTTT GCCAAGTGGT TGGGAAATGC GTCTTACCAA TACTGCTCGT
    GTATATTTTG TTGATCACAA TACCAAGACT ACCACTTGGG ATGATCCTCG TTTACCTTCG
    TCGTTAGATC AAAACGTTCC TCAATACAAA CGTGATTTCC GTAGAAAGTT GATTTATTTC
    CTTTCGCAAC CAGCTTTGCA TCCTTTGCCA GGGCAGTGCC ACATTAAAGT GCGTAGAAAT
    CATATCTTTG AAGATTCGTA TGCGGAAATT ATGAGACAAT CTGCAACCGA TTTGAAAAAA
    CGTTTGATGA TTAAGTTTGA TGGTGAAGAT GGTTTGGATT ACGGTGGATT ATCCCGTGAA
    TACTTCTTTT TGTTATCACA TGAAATGTTC AACCCCTTTT ATTGTTTATT TGAATACTCT
    TCGGTTGATA ATTATACGCT TCAAATTAAT CCTCATTCTG GCATTAATCC AGAGCATTTG
    AACTATTTCA AGTTCATAGG CCGAGTCATT GGTCTCGCAA TTTTCCATCG TCGGTTTGTT
    GATGCCTTTT TCGTTGTTTC TTTTTACAAA ATGATTTTAC AAAAGAAGGT GACGTTACAG
    GATATGGAAA GTATGGATGC AGAGTATTAT CGAAGTTTAG TCTGGATTTT GGACAACGAT
    ATAACCGGTG TTCTTGATTT GACCTTTAGT GTCGAAGACA ATTGTTTTGG CGAGGTTGTT
    ACGATTGATT TGAAGCCGAA TGGTCGAAAC ATTGAAGTTA CAGAAGAGAA TAAACGCGAA
    TATGTTGATT TGGTGACTGT ATGGCGCATT CAAAAACGTA TAGAAGAGCA GTTTAATGCA
    TTTCATGAAG GTTTTAGTGA GCTCATACCA CAGGAACTGA TTAACGTGTT TGACGAGAGA
    GAATTGGAGT TGTTGATTGG AGGCATTTCC GAAATTGACA TGGAGGATTG GAAGAAGCAT
    ACGGATTATC GTTCATACAG TGAAAATGAC CAGATTATTA AATGGTTTTG GGAACTTATG
    GATGAATGGA GTAATGAAAA GAAATCCAGA CTTTTACAAT TTACCACTGG TACTAGCCGA
    ATTCCTGTCA ACGGGTTCAA AGATTTGCAA GGAAGTGATG GCCCCCGTAA GTTTACTATT
    GAAAAAGCTG GTGAACCCAA TAAACTTCCC AAGGCCCACA CCTGTTTCAA TCGACTTGAT
    CTTCCTCCTT ATACTTCGAA AAAAGATTTG GATCATAAAT TGTCCATAGC TGTTGAAGAG
    ACGATTGGTT TTGGTCAGGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_594396.1
    CDS266..2569
    Translation

    Target ORF information:

    RefSeq Version NM_001019819.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe HECT-type ubiquitin-protein ligase E3 Pub1 (pub1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001019819.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCAAACT CAGCTCAATC TCGTCGAATT CGAGTAACAA TTGTTGCTGC TGATGGCCTT 
    TACAAACGAG ATGTGTTTCG TTTTCCTGAC CCGTTTGCGG TACTAACAGT GGATGGTGAG
    CAAACACATA CCACAACTGC TATCAAAAAG ACGTTAAACC CTTATTGGAA TGAGACTTTT
    GAAGTTAATG TTACAGATAA TAGCACCATT GCGATTCAGG TGTTCGATCA GAAAAAATTT
    AAGAAAAAAG GCCAAGGCTT TCTAGGAGTG ATAAATCTTC GTGTTGGAGA TGTGCTCGAT
    CTCGCCATTG GAGGTGATGA AATGTTGACC CGCGATTTGA AAAAGTCAAA TGAGAATACA
    GTAGTTCATG GCAAGATAAT CATAAACTTA TCAACGACTG CGCAGTCAAC CTTACAGGTT
    CCATCCAGTG CAGCATCAGG TGCACGTACC CAACGTACTA GCATTACCAA TGACCCGCAA
    AGCTCACAAA GTAGCAGTGT GTCTCGTAAC CCTGCTTCCT CTCGTGCTGG TTCACCTACC
    CGCGACAACG CACCTGCTGC TTCCCCTGCT AGTTCAGAAC CTCGCACATT TAGTTCATTT
    GAAGATCAAT ATGGGCGCCT TCCTCCTGGA TGGGAAAGAC GTACCGATAA TCTTGGCCGA
    ACTTATTATG TAGATCATAA TACAAGAAGC ACAACGTGGA TTCGCCCCAA CCTAAGTTCT
    GTTGCCGGAG CAGCCGCAGC CGAATTGCAT AGTAGTGCAT CGTCTGCGAA TGTTACCGAA
    GGTGTTCAAC CTTCCTCTAG CAATGCAGCT CGTCGTACAG AAGCTAGTGT TTTGACCTCT
    AACGCTACTA CTGCTGGTTC AGGAGAGCTT CCACCGGGAT GGGAGCAAAG GTATACACCA
    GAGGGTCGAC CCTACTTTGT GGATCATAAT ACGCGAACTA CTACTTGGGT GGATCCGCGC
    CGTCAACAGT ACATTCGTTC CTATGGCGGT CCTAATAATG CTACTATTCA GCAACAACCT
    GTCTCTCAAC TTGGTCCTTT GCCAAGTGGT TGGGAAATGC GTCTTACCAA TACTGCTCGT
    GTATATTTTG TTGATCACAA TACCAAGACT ACCACTTGGG ATGATCCTCG TTTACCTTCG
    TCGTTAGATC AAAACGTTCC TCAATACAAA CGTGATTTCC GTAGAAAGTT GATTTATTTC
    CTTTCGCAAC CAGCTTTGCA TCCTTTGCCA GGGCAGTGCC ACATTAAAGT GCGTAGAAAT
    CATATCTTTG AAGATTCGTA TGCGGAAATT ATGAGACAAT CTGCAACCGA TTTGAAAAAA
    CGTTTGATGA TTAAGTTTGA TGGTGAAGAT GGTTTGGATT ACGGTGGATT ATCCCGTGAA
    TACTTCTTTT TGTTATCACA TGAAATGTTC AACCCCTTTT ATTGTTTATT TGAATACTCT
    TCGGTTGATA ATTATACGCT TCAAATTAAT CCTCATTCTG GCATTAATCC AGAGCATTTG
    AACTATTTCA AGTTCATAGG CCGAGTCATT GGTCTCGCAA TTTTCCATCG TCGGTTTGTT
    GATGCCTTTT TCGTTGTTTC TTTTTACAAA ATGATTTTAC AAAAGAAGGT GACGTTACAG
    GATATGGAAA GTATGGATGC AGAGTATTAT CGAAGTTTAG TCTGGATTTT GGACAACGAT
    ATAACCGGTG TTCTTGATTT GACCTTTAGT GTCGAAGACA ATTGTTTTGG CGAGGTTGTT
    ACGATTGATT TGAAGCCGAA TGGTCGAAAC ATTGAAGTTA CAGAAGAGAA TAAACGCGAA
    TATGTTGATT TGGTGACTGT ATGGCGCATT CAAAAACGTA TAGAAGAGCA GTTTAATGCA
    TTTCATGAAG GTTTTAGTGA GCTCATACCA CAGGAACTGA TTAACGTGTT TGACGAGAGA
    GAATTGGAGT TGTTGATTGG AGGCATTTCC GAAATTGACA TGGAGGATTG GAAGAAGCAT
    ACGGATTATC GTTCATACAG TGAAAATGAC CAGATTATTA AATGGTTTTG GGAACTTATG
    GATGAATGGA GTAATGAAAA GAAATCCAGA CTTTTACAAT TTACCACTGG TACTAGCCGA
    ATTCCTGTCA ACGGGTTCAA AGATTTGCAA GGAAGTGATG GCCCCCGTAA GTTTACTATT
    GAAAAAGCTG GTGAACCCAA TAAACTTCCC AAGGCCCACA CCTGTTTCAA TCGACTTGAT
    CTTCCTCCTT ATACTTCGAA AAAAGATTTG GATCATAAAT TGTCCATAGC TGTTGAAGAG
    ACGATTGGTT TTGGTCAGGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L