cdc20 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	cdc20
Entrez Gene ID	2540627
Full Name	DNA polymerase epsilon catalytic subunit Pol2
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022274.2	NP_596354.1	DNA polymerase epsilon catalytic subunit Pol2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Disruption of Dos1, Dos2, or Cdc20 results in dissociation of CENP-A from centromeres and mislocalization of the protein to noncentromeric sites during S phase.
Title: Cell cycle-dependent deposition of CENP-A requires the Dos1/2-Cdc20 complex.

Cdc20 and Dpb2 of the Pol epsilon complex are required for loading of GINS, Cut5, Drc1, and Cdc45 onto replication origins.
Title: DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.

Collectively, these data and earlier results in budding yeast indicate that the major roles of Pol-delta and Pol-epsilon at the eukaryotic replication fork are evolutionarily conserved.
Title: The major roles of DNA polymerases epsilon and delta at the eukaryotic replication fork are evolutionarily conserved.

GINS functions in a pathway that involves Cdc45 and is necessary for DNA polymerase epsilon chromatin binding, but a separate pathway sets up the chromatin association of DNA polymerase alpha.
Title: GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.

the Mad3 KEN box is a critical mediator of Cdc20-APC/C inhibition, without which neither Mad3 nor Mad2 can associate with the APC/C or inhibit anaphase onset
Title: The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).

The following cdc20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cdc20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100229	NM_001022274.2 Latest version!	Schizosaccharomyces pombe DNA polymerase epsilon catalytic subunit Pol2 (cdc20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cdc20 ( NM_001022274.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596354.1 Schizosaccharomyces pombe DNA polymerase epsilon catalytic subunit Pol2 (cdc20), mRNA.

Price & Availability↑

CloneID	OSc100229
Clone ID Related Accession (Same CDS sequence)	NM_001022274.2
Accession Version	NM_001022274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	DNA polymerase epsilon catalytic subunit Pol2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022274.1.

ATGCCCTTAA AAACAGCTCG AGGAGCATCC AAATATCAGT TTAGGAAATT TAATGGTAAC 
TACAATGGAA AAAGTAAATC GAACGGTAGG ACGTTTGCAA AATCAACCGA GGAAGTTGGC 
TTCAATGATC CTATGAAAAT TGTCTACAAA AAGAATGAGA TTGATCGAAT GATGGGTTTT 
GATAGCTATG AAGGTGGACA ACCACGAGAA GCTTGGCTTC TCAACGTTCA TCCAACAGTA 
ATCGAAAGCA CCAAAGGCAA TTCTACCCTC AGTGCCGTTG ACTTTTACTT CATTCAAGAC 
GATGGTGACA CTTTCCGGTG TACCATTCCA TACTCTCCTT ACTTCTACAT TGCTGCTCGA 
GAAGGTAAAG AAGCTTTGGT TGACGATTAT TTAAAAAAAA AATTTGTGGG CTTAATTAAA 
TCTACTACGC GTATCTTTAA AGAAGATTTA CAGCTAAAAA ACCACATAGT GGGTTACCAA 
AAGTTGTATA TAAAGTTGGT TTTTGATAAC CTGAATGACC TTCAGGCTGT CAGGAAGTCT 
TTGATGTCTG CTGTGAAAGC TAATTCTTCC CAACAAGATG CCGTTGATGC ATACACTAAC 
TTGTCTTCGG AGAATTTAAA CGGGATTATA GAAAACGCAT TTGAAGATCC TTTAAATCAT 
GTTTTAGATA TCCGTGAGTA CGATGTTCCG TATCATTCTC GTACACTTAT TGATTTGAAT 
ATTCGAGTCG GACAATGGTA TACTGTTTCA TATCATGAAG GCCATGTTCA AATATCCCTT 
TTAGCTTCCC GAATCGAGCG TGCAGAACCT ACTATAATGG CTTTTGATAT TGAAACCACT 
AAGCTGCCAT TGAAGTTTCC TGATAGCTCG TTCGATAAAA TAATGATGAT ATCTTATATG 
ATTGATGGCC AAGGATTTTT AATTACCAAT AGAGAAATTA TTTCCCAGAA TATTGAAGAT 
TTTCATTATA CTCCGAGGGA GGAATTTGAG GGCCCATTTA TTATTTTTAA TGAGCCTGAT 
GAAGTTGGCC TACTTCATCG ATTCTTTAAA CATATAAGGT CTGCAAAGCC TTCAGTAATA 
GTTACATACA ATGGTGACTT CTTTGACTGG CCATTTGTAG ACGCACGTGC TGCATTTCAC 
GGATTGAATT TAACAGAAGA AACTGGTTTC TTCAGGGACG CAGAGGATGA ATATAAGTCT 
TCTTATTGTA GTCATATGGA TGCCTTCCGG TGGGTAAAGA GAGATAGTTA TTTACCTCAA 
GGAAGTCAAG GTCTCAAAGC TGTCACTGTC AGTAAATTAG GTTATAATCC TATCGAACTA 
GATCCAGAAT TGATGACTCC TTATGCAAGT GAAAAACCTC AAGTTCTTGC TCAATATTCA 
GTTTCAGATG CTGTTGCTAC TTATTTTCTT TACATGAAAT ATGTGCATCC TTTCATTTTT 
TCTCTTTGTA ACATTATCCC ATTAAATCCT GATGAAGTTT TACGTAAAGG CACGGGTACG 
TTGTGTGAAA CGCTTTTAAC TGTTGAGGCT TGTACAAAAA ATATTATTCT TCCGAATAAA 
CATGTTGATG CTAGTCAAAA ATTTTTCGAC GGTCATTTAC TTGCAAGTGA GACTTATGTT 
GGAGGACATG TTGAAAGTCT TGAAAGTGGT GTTTTTAGAA GCGACTTGCC GACTAATTTC 
AACATGGATC CAAAAGTTTA TGAAGAACTA ATTTTACAGT TGGACAAAGC CTTAGATTTT 
TCTTTAACAG TTGAAAACAA CGTCAATGTT GATGAAATCG AAAATTATGA AGAGGTTAGA 
GACAGCATAT TAAAGAAACT AAGTGACCTT CGTGATCGAC CAAAAAGGTC GGAGAAGCCA 
AGGATTTACC ATCTTGATGT CGCCTCAATG TACCCAAATA TTATGATTAC CAACCGGTTA 
CAGCCAGATT CAGTAAAAGA TGAATCGTTT TGTGCAACAT GCGATCTTAA TGTGCCCAAT 
AAGACATGTG ATCGTCGGAT GGTATGGGCA TGGAGAGGGG AGTATTACCC TGCTAAAAAA 
GGTGAATATC ATATGATATA TTCTGCTTTA CAATCTGAGC GTTTTCCAGG CCCTACCCCT 
TTCTCACCTT TTAGGTCATT TCAAGAGCTT TCTCCCTCAG AACAAGCTGC TATGGTGCAA 
AAGCGCATTG CGGATTACAG CCGTAAAGTG TATCACCGTC TATATGATAA TACCGTTATC 
GAAAGGGAAA CTATAATTTG TCAAAAGGAA AACTCATTTT ATATTGATAC GGTAAAAAGC 
TTTAGGGATC GCCGTTATGA CTTTAAAGGA TTGCAAAAAA AATGGGTCAA ACAACTTGCT 
GCTATAAAGG AAAAAGGAGG ACTTGCTGAA ATAGAGGAGG CCAAAAAGAT GGTCGTACTC 
TACGATTCTT TACAGTTAGC TCACAAAGTT ATTTTAAACT CTTTCTATGG ATATGTCATG 
CGAAAAGGCT CCCGTTGGTA TTCGATTGAA ATGGCTGGTA TCACATGTTT GACAGGCGCT 
ACGATTATTC AGATGGCTCG TCAAATAGTT GAACGTGCAG GTAGGCCATT GGAACTTGAC 
ACAGATGGTA TTTGGTGTAT TCTTCCAGAA TCGTTTCCTG AAAACTTTGA ATTTAAAAAG 
AAATCCGGAG GAAAGGTTTT TATCTCTTAT CCATGCGTTA TGTTAAATCA CCTGGTTCAT 
GAAAAGTTCA CCAATCATCA ATATTCTGCC TTAAAGGACC CAGAGAAATT GGTGTATGAA 
ACAACTAGTG AAAACAGCAT TTTTTTTGAG GTTGATGGTC CTTATAGAGC TATGATTTTG 
CCTGCTTCAA CGGAAGAAGG TAAAAACTTA AAAAAGCGTT ATGCCGTTTT CAATTTCGAT 
GGTAGTTTAG CTGAACTTAA AGGCTTTGAG GTGAAAAGAA GAGGAGAATT GAAGCTCATT 
AAGGATTTTC AATCTCAGAT ATTTAAAGTT TTTTTGAAAG GTGATTCTTT AGAGGAATGC 
TATCAAGAAG TTGCCTATGT TGCTGACACT TGGTTAGAGA TTCTATTCAC CAAAGGCTCT 
AACCTAACAG ATGATGAATT AATTGAACTG ATTTCTGAGA ATCGTAGTAT GTCTAAAGCT 
TTGTCTGAAT ACGGAAGCCA AAAATCGACA TCCATTACTA CAGCCAGGCG ACTGGCTGAC 
TTTTTGGGTG ATCAAATGAC CAAAGACAAA GGTCTAGCAT GTCGTTTTAT TATTTCCGCA 
AGTCCAAAAG GAAGGCCAGT TGCAGAAAGG GCAGTACCGG TAGCTATTTT CTTTGCTGAA 
GAATCTGTTA AGCGACATTT TCTTAGGCTC TGGTTGAAAG ATAATGGTCT ATATGATGTT 
GATATTAGAG ACATTATTGA TTGGGATTAT TATTTGAAAC GTCTAGGATC AGTAGTACAA 
AAACTAATTA GCATCCCTGC TGCTCTCCAA AGGATTTCAA ACCCCGTTAC CCGTTTTCCA 
TTGCCGGACT GGCTTCAAAA ACGAGTTGCT GTCTTGAATT CTAAATACCA ACAGAAAAAG 
ATCGATTCCA TTTTCTCCTT AGCCCCTACA AATCCATCCA CAATAAACAA CACAAAAGTT 
ACTGATATTG AGGATTTAGG AAGTGTGACT CACAAAGATA AGCGTATTGT CGCTCGAGTT 
ACCAAGCGTA AACTTTTACA ACAATCTGGG AACTCGGAAG CACCTGTATC TTTTGAAGTA 
AAACCTGTAT CCTTTATGGA TGGATATTCG AATTGGCTTA AGTATGCTAA GAAAAAGTGG 
AAATATCAGA AGCAGGTCAA GCTTCGAAGA AGACATTTAA TTGGGTTTCA ATCAAGGCAA 
TTCACCAATG TTTTACAATC TTCTGCTGAA GTTATGTTTG AAAATTTATG GCATATTTTA 
CAAATACGAG AAACCGATGT TCCTGGTATT TTACATGCTT GGGTAATTAT TCGTAATCGG 
TTGACCTCGA TACGGTTCAT TGTGAATAGA AAGTTTTTTG TATGTTTCAA AGATGAGACT 
TTACCGAATG TTGAAATTGA GGGATGCTTA ATTGAAAAAA GCAATGCGAT ATTACCGCAT 
GGAAGCACTT CGGATAAGCT TTTTTTACTT GAAATTCCAG AAAAGAGCTA TTTAACGGAA 
AAGGTATCAA TTTCTATGAT TTTTGCCCAT CCGTCTGTTA GTGGAATATA TGAGACTCGT 
ATAGAACCAA TTGAAAGATT GATATTAGAA ATGGGATCCA GAAAAAGGTT TAATAATTCA 
GTACCTGGAG CGTTAGGTAA AGGCTTCGAA TTTGGATTTG AATCTAAAAT GTTCACTGAC 
CCAAGTGATA ACGATGTTTC CTATCTTGAT GGAGTAGAGA TGAACTACCT TTATGCATTC 
CATTTTTCCA TTAGTAATAG GTTTGTTTTT TCACTTTTTA TGCCACATTT AAAAAAAGTT 
GAGGCCATAA TTTATGACAA ACTGCCAGGT AGTGATATGT CTTTCCCATC AATCAGCAAA 
ATTTACGAAG AGCTACGGTC GAAATTTGAT AATTTAATTA AGGAAAGTAG TATCGAATAC 
CCTGATACTC TTTCGTGTAA TGTCATTTTC TCGGGAAACG AAAGGAAAGC GTACAAACTG 
ATAGATGAAA AGTTGCTTCA ATATTTTTCA ACTAAAACTA AAAATAGTCT GCTTATTATT 
GAAAGTTCAC TCCCTCACAT ATTGAAGGCA AATGTGAAGC AAATTGAAGA GCTTCCGTAT 
ATAATGATAC CACGGCTTGA GAGCAACATA CAGTCTCTTT CTTGGAAACA ACACATTGCT 
ACGAAAATGA TCCAACATTT CTTAGCTATT GGTAGCTGGC TTTTCCATCG CATACAACTT 
TCTCGCTTCT CAGATATCCC ATTATGCAAC TTTGAAAGCG ATGACATTCA GTACAGTATC 
GATGTTGTTT ACTCTAGAAA GCTGAAAGAA CATAATATTA TCCTTTGGTG GAATAAAGGC 
CCAACTCCAG ACTTAGGTGG TATTGAGAAG GATAGTATTC TGCAGATTGC CAGTCCCAAA 
GATCCACTAG AAGTTAACAA TCCTGGTGCT TATTCAAATG CTTGTGTTGA CATTTCTCTT 
TCCAACCTTG CACTTTGTAG CATTCTCAAT TCTGCTCTCA TAAATGACAT AGAAGGTATT 
GGCGACATGG CAGCTTTGAA TGATAATTAC ATGACCGCCA TAAATGATGA TCTGGAGGAA 
AAGTTGGGAA TACATGATAA TATTGGACTG ACACATTCAT TGCCGGTGTT GAAGGCGTTG 
GTTAAAACTT GGTGGAACGA AGCTGCATCC GGTAATAATC TCGCTGATCT TATAATTCAA 
CATTTAGCCC GATGGATTTC TTCATCAAAA TCTTATCTGT ACAGTCCTTT ACTCTCCTCG 
CACGTGGAAG TTATAATGAG AAAAACTTTT TTACAACTTC TCTCTGAGAT AAAACGCCTT 
GGAGCACATA TCATACACGC TTCTGCTAAT AAAATATTAA TCAAAACGAG CAAATTAATC 
GTCCAGAACG CTGTCACTTA TTCAAATTAT TTACTCAAAT CCATTAAGAC GTTACCACTA 
TTTCACTTTT TGGATCTAAA TGTCACGGAA TACTGGGATT ATCTTTTATG GATGGATTCC 
GTTAATTATG GAGGAAAAAT GGTGGCAGCA AATTTTTCAG CTACAAATGA AGAACCACAG 
ACAGTTGTTT CATGGCACAT TAAAAGTCAT TTACCTCCAA TCATTCAGCC CGAATTTCAG 
AGTTGGATTG TTGAGTTTAT TGAAGAGGTT TATAAGCAAA AATTAGAAAA GTCTAATACA 
AAAGTTGGGT TTGTTCGCGT GAAAAACAAC AATGCTGATG AGGACTCTGA AATAGTTGGA 
TCGGGAATTT TAAAAAGTAA GTTGATACAT CCTCTTAAAC GGAAAGTTGC TCAAGTAAGG 
AGATGTTTTC AAGAGCTTCA GCTTGATGAA AATACCAGAG AAGATCTTAA ATTTCCAAAG 
TTGCCAGGTT CTTTTCTTAA CTATACTGAT GGCGCTCTAG AATTGGTTAA AAGCATCTGT 
GCTGTTTTTG AACTCTCCCA TGATCTCAAT TTGGAGGTCC GTTTTTTAAA AAAGAGTTTA 
TTGTCACTTT TACAAATTCA AGAGTTTTCC ACTCAGGCGG TATTTCGATA TCCTAGTCGT 
CGACTTTCAC TTGATCAAAT ACCCTGTAAG CAGTGTGGCG TCCATCAGGA TTTTGACCTT 
TGTTTACATG AGCATCTATG GCCTACTCGC GATGACATGG GGACCTTGGT GTTTTCTGAC 
GGATGGTCTT GTTCCAGCTG TAACTTAGTT TACGATCGAT GGGTGTTTGA GGAAACTTTG 
GTGGATAACT TATACCATCA ATTGACATTA TATCAACTGC AAGATTTGAT TTGTTCTAAA 
TGTAAGACAG TTAAGCAATG GTCATTAAAG GAACGCTGTT CATGCAGCGG AGAATGGGTA 
TTACAGCTTT CACCTACAAA GTTTCGTGAA ATGTTAAACG TTTATCAATC TGTTGCTGAT 
TTTTACGAGT TTTCTATATT GCAAAATTCT GTTCAGTCCA TACTTTCTGT GCTGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596354.1
CDS	200..6799
Translation	MPLKTARGASKYQFRKFNGNYNGKSKSNGRTFAKSTEEVGFNDPMKIVYKKNEIDRMMGFDSYEGGQPREAWLLNVHPTVIESTKGNSTLSAVDFYFIQDDGDTFRCTIPYSPYFYIAAREGKEALVDDYLKKKFVGLIKSTTRIFKEDLQLKNHIVGYQKLYIKLVFDNLNDLQAVRKSLMSAVKANSSQQDAVDAYTNLSSENLNGIIENAFEDPLNHVLDIREYDVPYHSRTLIDLNIRVGQWYTVSYHEGHVQISLLASRIERAEPTIMAFDIETTKLPLKFPDSSFDKIMMISYMIDGQGFLITNREIISQNIEDFHYTPREEFEGPFIIFNEPDEVGLLHRFFKHIRSAKPSVIVTYNGDFFDWPFVDARAAFHGLNLTEETGFFRDAEDEYKSSYCSHMDAFRWVKRDSYLPQGSQGLKAVTVSKLGYNPIELDPELMTPYASEKPQVLAQYSVSDAVATYFLYMKYVHPFIFSLCNIIPLNPDEVLRKGTGTLCETLLTVEACTKNIILPNKHVDASQKFFDGHLLASETYVGGHVESLESGVFRSDLPTNFNMDPKVYEELILQLDKALDFSLTVENNVNVDEIENYEEVRDSILKKLSDLRDRPKRSEKPRIYHLDVASMYPNIMITNRLQPDSVKDESFCATCDLNVPNKTCDRRMVWAWRGEYYPAKKGEYHMIYSALQSERFPGPTPFSPFRSFQELSPSEQAAMVQKRIADYSRKVYHRLYDNTVIERETIICQKENSFYIDTVKSFRDRRYDFKGLQKKWVKQLAAIKEKGGLAEIEEAKKMVVLYDSLQLAHKVILNSFYGYVMRKGSRWYSIEMAGITCLTGATIIQMARQIVERAGRPLELDTDGIWCILPESFPENFEFKKKSGGKVFISYPCVMLNHLVHEKFTNHQYSALKDPEKLVYETTSENSIFFEVDGPYRAMILPASTEEGKNLKKRYAVFNFDGSLAELKGFEVKRRGELKLIKDFQSQIFKVFLKGDSLEECYQEVAYVADTWLEILFTKGSNLTDDELIELISENRSMSKALSEYGSQKSTSITTARRLADFLGDQMTKDKGLACRFIISASPKGRPVAERAVPVAIFFAEESVKRHFLRLWLKDNGLYDVDIRDIIDWDYYLKRLGSVVQKLISIPAALQRISNPVTRFPLPDWLQKRVAVLNSKYQQKKIDSIFSLAPTNPSTINNTKVTDIEDLGSVTHKDKRIVARVTKRKLLQQSGNSEAPVSFEVKPVSFMDGYSNWLKYAKKKWKYQKQVKLRRRHLIGFQSRQFTNVLQSSAEVMFENLWHILQIRETDVPGILHAWVIIRNRLTSIRFIVNRKFFVCFKDETLPNVEIEGCLIEKSNAILPHGSTSDKLFLLEIPEKSYLTEKVSISMIFAHPSVSGIYETRIEPIERLILEMGSRKRFNNSVPGALGKGFEFGFESKMFTDPSDNDVSYLDGVEMNYLYAFHFSISNRFVFSLFMPHLKKVEAIIYDKLPGSDMSFPSISKIYEELRSKFDNLIKESSIEYPDTLSCNVIFSGNERKAYKLIDEKLLQYFSTKTKNSLLIIESSLPHILKANVKQIEELPYIMIPRLESNIQSLSWKQHIATKMIQHFLAIGSWLFHRIQLSRFSDIPLCNFESDDIQYSIDVVYSRKLKEHNIILWWNKGPTPDLGGIEKDSILQIASPKDPLEVNNPGAYSNACVDISLSNLALCSILNSALINDIEGIGDMAALNDNYMTAINDDLEEKLGIHDNIGLTHSLPVLKALVKTWWNEAASGNNLADLIIQHLARWISSSKSYLYSPLLSSHVEVIMRKTFLQLLSEIKRLGAHIIHASANKILIKTSKLIVQNAVTYSNYLLKSIKTLPLFHFLDLNVTEYWDYLLWMDSVNYGGKMVAANFSATNEEPQTVVSWHIKSHLPPIIQPEFQSWIVEFIEEVYKQKLEKSNTKVGFVRVKNNNADEDSEIVGSGILKSKLIHPLKRKVAQVRRCFQELQLDENTREDLKFPKLPGSFLNYTDGALELVKSICAVFELSHDLNLEVRFLKKSLLSLLQIQEFSTQAVFRYPSRRLSLDQIPCKQCGVHQDFDLCLHEHLWPTRDDMGTLVFSDGWSCSSCNLVYDRWVFEETLVDNLYHQLTLYQLQDLICSKCKTVKQWSLKERCSCSGEWVLQLSPTKFREMLNVYQSVADFYEFSILQNSVQSILSVLN

Target ORF information:

RefSeq Version	NM_001022274.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe DNA polymerase epsilon catalytic subunit Pol2 (cdc20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022274.2

ORF Insert Sequence:

ATGCCCTTAA AAACAGCTCG AGGAGCATCC AAATATCAGT TTAGGAAATT TAATGGTAAC 
TACAATGGAA AAAGTAAATC GAACGGTAGG ACGTTTGCAA AATCAACCGA GGAAGTTGGC 
TTCAATGATC CTATGAAAAT TGTCTACAAA AAGAATGAGA TTGATCGAAT GATGGGTTTT 
GATAGCTATG AAGGTGGACA ACCACGAGAA GCTTGGCTTC TCAACGTTCA TCCAACAGTA 
ATCGAAAGCA CCAAAGGCAA TTCTACCCTC AGTGCCGTTG ACTTTTACTT CATTCAAGAC 
GATGGTGACA CTTTCCGGTG TACCATTCCA TACTCTCCTT ACTTCTACAT TGCTGCTCGA 
GAAGGTAAAG AAGCTTTGGT TGACGATTAT TTAAAAAAAA AATTTGTGGG CTTAATTAAA 
TCTACTACGC GTATCTTTAA AGAAGATTTA CAGCTAAAAA ACCACATAGT GGGTTACCAA 
AAGTTGTATA TAAAGTTGGT TTTTGATAAC CTGAATGACC TTCAGGCTGT CAGGAAGTCT 
TTGATGTCTG CTGTGAAAGC TAATTCTTCC CAACAAGATG CCGTTGATGC ATACACTAAC 
TTGTCTTCGG AGAATTTAAA CGGGATTATA GAAAACGCAT TTGAAGATCC TTTAAATCAT 
GTTTTAGATA TCCGTGAGTA CGATGTTCCG TATCATTCTC GTACACTTAT TGATTTGAAT 
ATTCGAGTCG GACAATGGTA TACTGTTTCA TATCATGAAG GCCATGTTCA AATATCCCTT 
TTAGCTTCCC GAATCGAGCG TGCAGAACCT ACTATAATGG CTTTTGATAT TGAAACCACT 
AAGCTGCCAT TGAAGTTTCC TGATAGCTCG TTCGATAAAA TAATGATGAT ATCTTATATG 
ATTGATGGCC AAGGATTTTT AATTACCAAT AGAGAAATTA TTTCCCAGAA TATTGAAGAT 
TTTCATTATA CTCCGAGGGA GGAATTTGAG GGCCCATTTA TTATTTTTAA TGAGCCTGAT 
GAAGTTGGCC TACTTCATCG ATTCTTTAAA CATATAAGGT CTGCAAAGCC TTCAGTAATA 
GTTACATACA ATGGTGACTT CTTTGACTGG CCATTTGTAG ACGCACGTGC TGCATTTCAC 
GGATTGAATT TAACAGAAGA AACTGGTTTC TTCAGGGACG CAGAGGATGA ATATAAGTCT 
TCTTATTGTA GTCATATGGA TGCCTTCCGG TGGGTAAAGA GAGATAGTTA TTTACCTCAA 
GGAAGTCAAG GTCTCAAAGC TGTCACTGTC AGTAAATTAG GTTATAATCC TATCGAACTA 
GATCCAGAAT TGATGACTCC TTATGCAAGT GAAAAACCTC AAGTTCTTGC TCAATATTCA 
GTTTCAGATG CTGTTGCTAC TTATTTTCTT TACATGAAAT ATGTGCATCC TTTCATTTTT 
TCTCTTTGTA ACATTATCCC ATTAAATCCT GATGAAGTTT TACGTAAAGG CACGGGTACG 
TTGTGTGAAA CGCTTTTAAC TGTTGAGGCT TGTACAAAAA ATATTATTCT TCCGAATAAA 
CATGTTGATG CTAGTCAAAA ATTTTTCGAC GGTCATTTAC TTGCAAGTGA GACTTATGTT 
GGAGGACATG TTGAAAGTCT TGAAAGTGGT GTTTTTAGAA GCGACTTGCC GACTAATTTC 
AACATGGATC CAAAAGTTTA TGAAGAACTA ATTTTACAGT TGGACAAAGC CTTAGATTTT 
TCTTTAACAG TTGAAAACAA CGTCAATGTT GATGAAATCG AAAATTATGA AGAGGTTAGA 
GACAGCATAT TAAAGAAACT AAGTGACCTT CGTGATCGAC CAAAAAGGTC GGAGAAGCCA 
AGGATTTACC ATCTTGATGT CGCCTCAATG TACCCAAATA TTATGATTAC CAACCGGTTA 
CAGCCAGATT CAGTAAAAGA TGAATCGTTT TGTGCAACAT GCGATCTTAA TGTGCCCAAT 
AAGACATGTG ATCGTCGGAT GGTATGGGCA TGGAGAGGGG AGTATTACCC TGCTAAAAAA 
GGTGAATATC ATATGATATA TTCTGCTTTA CAATCTGAGC GTTTTCCAGG CCCTACCCCT 
TTCTCACCTT TTAGGTCATT TCAAGAGCTT TCTCCCTCAG AACAAGCTGC TATGGTGCAA 
AAGCGCATTG CGGATTACAG CCGTAAAGTG TATCACCGTC TATATGATAA TACCGTTATC 
GAAAGGGAAA CTATAATTTG TCAAAAGGAA AACTCATTTT ATATTGATAC GGTAAAAAGC 
TTTAGGGATC GCCGTTATGA CTTTAAAGGA TTGCAAAAAA AATGGGTCAA ACAACTTGCT 
GCTATAAAGG AAAAAGGAGG ACTTGCTGAA ATAGAGGAGG CCAAAAAGAT GGTCGTACTC 
TACGATTCTT TACAGTTAGC TCACAAAGTT ATTTTAAACT CTTTCTATGG ATATGTCATG 
CGAAAAGGCT CCCGTTGGTA TTCGATTGAA ATGGCTGGTA TCACATGTTT GACAGGCGCT 
ACGATTATTC AGATGGCTCG TCAAATAGTT GAACGTGCAG GTAGGCCATT GGAACTTGAC 
ACAGATGGTA TTTGGTGTAT TCTTCCAGAA TCGTTTCCTG AAAACTTTGA ATTTAAAAAG 
AAATCCGGAG GAAAGGTTTT TATCTCTTAT CCATGCGTTA TGTTAAATCA CCTGGTTCAT 
GAAAAGTTCA CCAATCATCA ATATTCTGCC TTAAAGGACC CAGAGAAATT GGTGTATGAA 
ACAACTAGTG AAAACAGCAT TTTTTTTGAG GTTGATGGTC CTTATAGAGC TATGATTTTG 
CCTGCTTCAA CGGAAGAAGG TAAAAACTTA AAAAAGCGTT ATGCCGTTTT CAATTTCGAT 
GGTAGTTTAG CTGAACTTAA AGGCTTTGAG GTGAAAAGAA GAGGAGAATT GAAGCTCATT 
AAGGATTTTC AATCTCAGAT ATTTAAAGTT TTTTTGAAAG GTGATTCTTT AGAGGAATGC 
TATCAAGAAG TTGCCTATGT TGCTGACACT TGGTTAGAGA TTCTATTCAC CAAAGGCTCT 
AACCTAACAG ATGATGAATT AATTGAACTG ATTTCTGAGA ATCGTAGTAT GTCTAAAGCT 
TTGTCTGAAT ACGGAAGCCA AAAATCGACA TCCATTACTA CAGCCAGGCG ACTGGCTGAC 
TTTTTGGGTG ATCAAATGAC CAAAGACAAA GGTCTAGCAT GTCGTTTTAT TATTTCCGCA 
AGTCCAAAAG GAAGGCCAGT TGCAGAAAGG GCAGTACCGG TAGCTATTTT CTTTGCTGAA 
GAATCTGTTA AGCGACATTT TCTTAGGCTC TGGTTGAAAG ATAATGGTCT ATATGATGTT 
GATATTAGAG ACATTATTGA TTGGGATTAT TATTTGAAAC GTCTAGGATC AGTAGTACAA 
AAACTAATTA GCATCCCTGC TGCTCTCCAA AGGATTTCAA ACCCCGTTAC CCGTTTTCCA 
TTGCCGGACT GGCTTCAAAA ACGAGTTGCT GTCTTGAATT CTAAATACCA ACAGAAAAAG 
ATCGATTCCA TTTTCTCCTT AGCCCCTACA AATCCATCCA CAATAAACAA CACAAAAGTT 
ACTGATATTG AGGATTTAGG AAGTGTGACT CACAAAGATA AGCGTATTGT CGCTCGAGTT 
ACCAAGCGTA AACTTTTACA ACAATCTGGG AACTCGGAAG CACCTGTATC TTTTGAAGTA 
AAACCTGTAT CCTTTATGGA TGGATATTCG AATTGGCTTA AGTATGCTAA GAAAAAGTGG 
AAATATCAGA AGCAGGTCAA GCTTCGAAGA AGACATTTAA TTGGGTTTCA ATCAAGGCAA 
TTCACCAATG TTTTACAATC TTCTGCTGAA GTTATGTTTG AAAATTTATG GCATATTTTA 
CAAATACGAG AAACCGATGT TCCTGGTATT TTACATGCTT GGGTAATTAT TCGTAATCGG 
TTGACCTCGA TACGGTTCAT TGTGAATAGA AAGTTTTTTG TATGTTTCAA AGATGAGACT 
TTACCGAATG TTGAAATTGA GGGATGCTTA ATTGAAAAAA GCAATGCGAT ATTACCGCAT 
GGAAGCACTT CGGATAAGCT TTTTTTACTT GAAATTCCAG AAAAGAGCTA TTTAACGGAA 
AAGGTATCAA TTTCTATGAT TTTTGCCCAT CCGTCTGTTA GTGGAATATA TGAGACTCGT 
ATAGAACCAA TTGAAAGATT GATATTAGAA ATGGGATCCA GAAAAAGGTT TAATAATTCA 
GTACCTGGAG CGTTAGGTAA AGGCTTCGAA TTTGGATTTG AATCTAAAAT GTTCACTGAC 
CCAAGTGATA ACGATGTTTC CTATCTTGAT GGAGTAGAGA TGAACTACCT TTATGCATTC 
CATTTTTCCA TTAGTAATAG GTTTGTTTTT TCACTTTTTA TGCCACATTT AAAAAAAGTT 
GAGGCCATAA TTTATGACAA ACTGCCAGGT AGTGATATGT CTTTCCCATC AATCAGCAAA 
ATTTACGAAG AGCTACGGTC GAAATTTGAT AATTTAATTA AGGAAAGTAG TATCGAATAC 
CCTGATACTC TTTCGTGTAA TGTCATTTTC TCGGGAAACG AAAGGAAAGC GTACAAACTG 
ATAGATGAAA AGTTGCTTCA ATATTTTTCA ACTAAAACTA AAAATAGTCT GCTTATTATT 
GAAAGTTCAC TCCCTCACAT ATTGAAGGCA AATGTGAAGC AAATTGAAGA GCTTCCGTAT 
ATAATGATAC CACGGCTTGA GAGCAACATA CAGTCTCTTT CTTGGAAACA ACACATTGCT 
ACGAAAATGA TCCAACATTT CTTAGCTATT GGTAGCTGGC TTTTCCATCG CATACAACTT 
TCTCGCTTCT CAGATATCCC ATTATGCAAC TTTGAAAGCG ATGACATTCA GTACAGTATC 
GATGTTGTTT ACTCTAGAAA GCTGAAAGAA CATAATATTA TCCTTTGGTG GAATAAAGGC 
CCAACTCCAG ACTTAGGTGG TATTGAGAAG GATAGTATTC TGCAGATTGC CAGTCCCAAA 
GATCCACTAG AAGTTAACAA TCCTGGTGCT TATTCAAATG CTTGTGTTGA CATTTCTCTT 
TCCAACCTTG CACTTTGTAG CATTCTCAAT TCTGCTCTCA TAAATGACAT AGAAGGTATT 
GGCGACATGG CAGCTTTGAA TGATAATTAC ATGACCGCCA TAAATGATGA TCTGGAGGAA 
AAGTTGGGAA TACATGATAA TATTGGACTG ACACATTCAT TGCCGGTGTT GAAGGCGTTG 
GTTAAAACTT GGTGGAACGA AGCTGCATCC GGTAATAATC TCGCTGATCT TATAATTCAA 
CATTTAGCCC GATGGATTTC TTCATCAAAA TCTTATCTGT ACAGTCCTTT ACTCTCCTCG 
CACGTGGAAG TTATAATGAG AAAAACTTTT TTACAACTTC TCTCTGAGAT AAAACGCCTT 
GGAGCACATA TCATACACGC TTCTGCTAAT AAAATATTAA TCAAAACGAG CAAATTAATC 
GTCCAGAACG CTGTCACTTA TTCAAATTAT TTACTCAAAT CCATTAAGAC GTTACCACTA 
TTTCACTTTT TGGATCTAAA TGTCACGGAA TACTGGGATT ATCTTTTATG GATGGATTCC 
GTTAATTATG GAGGAAAAAT GGTGGCAGCA AATTTTTCAG CTACAAATGA AGAACCACAG 
ACAGTTGTTT CATGGCACAT TAAAAGTCAT TTACCTCCAA TCATTCAGCC CGAATTTCAG 
AGTTGGATTG TTGAGTTTAT TGAAGAGGTT TATAAGCAAA AATTAGAAAA GTCTAATACA 
AAAGTTGGGT TTGTTCGCGT GAAAAACAAC AATGCTGATG AGGACTCTGA AATAGTTGGA 
TCGGGAATTT TAAAAAGTAA GTTGATACAT CCTCTTAAAC GGAAAGTTGC TCAAGTAAGG 
AGATGTTTTC AAGAGCTTCA GCTTGATGAA AATACCAGAG AAGATCTTAA ATTTCCAAAG 
TTGCCAGGTT CTTTTCTTAA CTATACTGAT GGCGCTCTAG AATTGGTTAA AAGCATCTGT 
GCTGTTTTTG AACTCTCCCA TGATCTCAAT TTGGAGGTCC GTTTTTTAAA AAAGAGTTTA 
TTGTCACTTT TACAAATTCA AGAGTTTTCC ACTCAGGCGG TATTTCGATA TCCTAGTCGT 
CGACTTTCAC TTGATCAAAT ACCCTGTAAG CAGTGTGGCG TCCATCAGGA TTTTGACCTT 
TGTTTACATG AGCATCTATG GCCTACTCGC GATGACATGG GGACCTTGGT GTTTTCTGAC 
GGATGGTCTT GTTCCAGCTG TAACTTAGTT TACGATCGAT GGGTGTTTGA GGAAACTTTG 
GTGGATAACT TATACCATCA ATTGACATTA TATCAACTGC AAGATTTGAT TTGTTCTAAA 
TGTAAGACAG TTAAGCAATG GTCATTAAAG GAACGCTGTT CATGCAGCGG AGAATGGGTA 
TTACAGCTTT CACCTACAAA GTTTCGTGAA ATGTTAAACG TTTATCAATC TGTTGCTGAT 
TTTTACGAGT TTTCTATATT GCAAAATTCT GTTCAGTCCA TACTTTCTGT GCTGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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cdc20 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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