NCU02728 cDNA ORF clone, Neurospora crassa OR74A

The following NCU02728 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU02728 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf08394 XM_960020.2
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Neurospora crassa OR74A pre-mRNA splicing factor ATP-dependent RNA helicase prp16 (NCU02728), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf08394
    Clone ID Related Accession (Same CDS sequence) XM_960020.2
    Accession Version XM_960020.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product pre-mRNA splicing factor ATP-dependent RNA helicase prp16
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_960020.1. COMPLETENESS: incomplete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGTCGCG ACGATAATGA AGACCGGTCC TTCAAACGAA GAAAGATCGA TTTCAACCCA 
    CTTCGCTCCG ATGACCGCTT CTCAGATCTC CCGTCGCGCA CGCCACACAA GGGCCCCAGA
    GGTGCCAATG GCAACAATGG GTCCTTCAAC TCCCGAGCTT CAACTCCACG AGTCGGATCC
    AGGTATAGTG GCGGGGAGAC ACCTCGGCAG AAAGAGTTTG ACGGCCCCGA ACCCGGCGTA
    AACGACGACG ACGCAACCAA TGCCCTCGAT CGGGACTGGT ACGGCGGTGA CGATGACCTT
    GGCGGCCACA CGTTTGGCGA TGACAGCCAC AATCCCTTCG GCGATGAGGG CGCATGGGCA
    GCCCAGGAGA GGGAGGCGGC CCTGGCGGAG AAGAAAATTG GGCAGATGGC GCGCGGTATG
    AGCGTCCGGC AGATTCAGAA ACAAAAGGAT GCCGACGCCT GGGAGACAAA CCGGATGCTC
    ACCTCAGGCG TGGCGCAAAG GAGAGACTTG GGACAGGACT TTGAGGATGA CCAGGAAGGC
    ACCAGAGTGC ATCTTCTAGT GCACGACTTA CGACCTCCTT TTCTGGATGG ACGAACCATC
    TTCACAAAGC AGTTGGATCC GGTCCCCGCT GTGCGAGATT CTCAAAGCGA TATGGCTATC
    TTCGCTCGAA AAGGAAGCAA GGTGGTCAGG GAAAGGAGGA CACAACGGGA GCGCCAAAGA
    CAGGCGCAGG AGGCTACCAA GGTGGCAGGA ACTGCACTAG GCAACCTGAT GGGAGTCAAG
    GAGGAAGATA CCGACAGCGC CTTGCCTATC GCTGTGGAGG AGGAGGCCGG CAAAAGCAAA
    AACATGAACA AGTTCAGTGA ACACATGAAG GAGAACGAGG GTGCCAGCAA TTTCAGCCAA
    AGCAAGTCCC TCAAGGAACA ACGCGAATTT CTTCCTGCCT TTGCTGTACG GGAGGACCTG
    CTGCGTGTGA TCCGTGACAA TCAGGTTGTC ATTGTGATTG GAGAAACAGG CTCTGGTAAG
    ACGACACAGC TCACCCAGTT TCTCTACGAG GACGGCTACG GCAAGACGGG CATGATCGGT
    TGCACCCAGC CCAGGAGAGT CGCGGCCATG AGTGTAGCGA AGCGTGTTGC TGAGGAGATG
    GAGGTGAAAC TTGGCAGCAC GGTAGGCTAC GCCATCCGCT TCGAGGATTG CACGAGCAAG
    GATACGGTCA TCAAGTACAT GACGGATGGT GTTCTTCTAC GAGAATCGCT GAATGAACCA
    GACCTGGACC GCTACTCATG CGTTATCATG GACGAAGCTC ACGAAAGAGC CCTCAACACT
    GATGTCTTGA TGGGCTTGTT TAAGAAAATC CTTCAAAGAC GCCGCGACTT GAAGCTGATT
    GTCACGTCTG CGACGATGAA TGCAAAGCGG TTTTCAGACT TCTATGGTGG TGCACCCGAG
    TTTACCATTC CCGGAAGAAC CTTCCCTGTC GACATCATGT TCCATCGGTC ACCTGTCGAA
    GATTACGTGG ACCAGGCTGT GCAGCAAGTT CTGGCGATTC ACGTAGGAAA ACCAGCAGGC
    GATATCCTCG TTTTCATGAC CGGCCAGGAA GATATTGAGG TGACCTGCGA ACTCATCCGC
    GAGCGGCTCG ATGCCCTCAA CGACCCACCG AAACTCAGTA TCCTTCCCAT CTACAGTCAG
    ATGCCCGCAG ACTTGCAGGC CAAGATTTTC GACAGGGCTG CTCCGGGTGT CCGAAAGGTC
    ATCGTGGCCA CGAATATCGC GGAAACCAGT TTGACGGTGG ATGGTATCAT GTACGTGGTT
    GATGCAGGCT ATTCAAAGCT CAAGGTATAC AATCCACGAA TGGGTATGGA TACCCTTCAA
    ATTACGCCTA TTTCGCAAGC AAACGCCATG CAGCGCGCTG GTCGTGCGGG TCGCACTGGG
    CCTGGCCAAG CATATCATCT CTTCACCGAG AAGGCCTTCA AAGAGGAGAT GTACATGCAA
    ACCATTCCGG AGATTCAGAG AACGAATCTT AGCAATACCG TTCTACTCCT CAAGTCACTC
    GGTGTTAAAG ACCTCCTTGA TTTCGACTTT ATGGATCCAC CACCTCAGGA TACCATCAGT
    ACATCTCTCT TCGATTTGTG GGCGTTGGGA GCACTGGACA ACCTAGGTGA GCTCACCGAC
    TTGGGGCGGA AGATGAATGC GTTCCCGATG GACCCACCCC TTGCAAAACT GCTCATCACC
    TCGGAGGAGT ATGGGTGCAG CGAGGAAATG GTCACCATCG TTTCGATGCT TTCCGTTCCC
    AACGTATTCT ACCGGCCCAA AGAACGACAA GAGGAGTCAG ATGCGGCTCG CGAGAAGTTC
    TTTGTACCAG AATCAGACCA CTTGACCTAT CTGCACGTTT ACACACAATG GAAGGCCAAC
    GGGTATAACG ACGGTTGGTG CGTGCGACAC TTTTTGCATT CCAAGTCTCT CCGTCGTGCC
    AAGGAAGTCC GCGAGCAGCT CCTGGACATC ATGAAGATGC AGAATATGAA GATGACGAGT
    TGTGGCACCG ACTGGGATGT TATTCGCAAG TGCATTTGCT CTGGCTACTA TCACCAGGCA
    GCAAAGGTCA AGGGCATTGG GGAGTATATC AACTTGAGGA CCAGTGTCAC CGTCCAATTG
    CATCCGACAA GTGCGCTTTA TGGACTGGGC TTCTTGCCAG ACTACGTGGT GTACCACGAG
    CTGATCCTCA CAAGTAAAGA GTACATGTCG ACGGTGACCT CGGTAGACCC CCACTGGTTG
    GCGGATCTCG GTGGTGTATT TTATTCGGTC AAGGAGAAAG GATATTCGGC GCGGGAGAAG
    AGGATCACAG AGACGGAGTT CAACAGGAGA ATGGAGATTG AGGCGCGGAT GGCAGCGGAC
    AAGAAGCGGC AAGAGGATCA GCTGCACGAA GAAGAGCTTC AGCTTGAGAA GAAGGCGGCC
    CCGTCCAAGG ACAAGAAGGT GGTTAAAGCG GGTGCCGTGG TGAAGCCAGT CAGGAAACGT
    GCTGGAAGGG GATTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_965113.1
    CDS56..3073
    Translation

    Target ORF information:

    RefSeq Version XM_960020.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A pre-mRNA splicing factor ATP-dependent RNA helicase prp16 (NCU02728), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_960020.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGTCGCG ACGATAATGA AGACCGGTCC TTCAAACGAA GAAAGATCGA TTTCAACCCA 
    CTTCGCTCCG ATGACCGCTT CTCAGATCTC CCGTCGCGCA CGCCACACAA GGGCCCCAGA
    GGTGCCAATG GCAACAATGG GTCCTTCAAC TCCCGAGCTT CAACTCCACG AGTCGGATCC
    AGGTATAGTG GCGGGGAGAC ACCTCGGCAG AAAGAGTTTG ACGGCCCCGA ACCCGGCGTA
    AACGACGACG ACGCAACCAA TGCCCTCGAT CGGGACTGGT ACGGCGGTGA CGATGACCTT
    GGCGGCCACA CGTTTGGCGA TGACAGCCAC AATCCCTTCG GCGATGAGGG CGCATGGGCA
    GCCCAGGAGA GGGAGGCGGC CCTGGCGGAG AAGAAAATTG GGCAGATGGC GCGCGGTATG
    AGCGTCCGGC AGATTCAGAA ACAAAAGGAT GCCGACGCCT GGGAGACAAA CCGGATGCTC
    ACCTCAGGCG TGGCGCAAAG GAGAGACTTG GGACAGGACT TTGAGGATGA CCAGGAAGGC
    ACCAGAGTGC ATCTTCTAGT GCACGACTTA CGACCTCCTT TTCTGGATGG ACGAACCATC
    TTCACAAAGC AGTTGGATCC GGTCCCCGCT GTGCGAGATT CTCAAAGCGA TATGGCTATC
    TTCGCTCGAA AAGGAAGCAA GGTGGTCAGG GAAAGGAGGA CACAACGGGA GCGCCAAAGA
    CAGGCGCAGG AGGCTACCAA GGTGGCAGGA ACTGCACTAG GCAACCTGAT GGGAGTCAAG
    GAGGAAGATA CCGACAGCGC CTTGCCTATC GCTGTGGAGG AGGAGGCCGG CAAAAGCAAA
    AACATGAACA AGTTCAGTGA ACACATGAAG GAGAACGAGG GTGCCAGCAA TTTCAGCCAA
    AGCAAGTCCC TCAAGGAACA ACGCGAATTT CTTCCTGCCT TTGCTGTACG GGAGGACCTG
    CTGCGTGTGA TCCGTGACAA TCAGGTTGTC ATTGTGATTG GAGAAACAGG CTCTGGTAAG
    ACGACACAGC TCACCCAGTT TCTCTACGAG GACGGCTACG GCAAGACGGG CATGATCGGT
    TGCACCCAGC CCAGGAGAGT CGCGGCCATG AGTGTAGCGA AGCGTGTTGC TGAGGAGATG
    GAGGTGAAAC TTGGCAGCAC GGTAGGCTAC GCCATCCGCT TCGAGGATTG CACGAGCAAG
    GATACGGTCA TCAAGTACAT GACGGATGGT GTTCTTCTAC GAGAATCGCT GAATGAACCA
    GACCTGGACC GCTACTCATG CGTTATCATG GACGAAGCTC ACGAAAGAGC CCTCAACACT
    GATGTCTTGA TGGGCTTGTT TAAGAAAATC CTTCAAAGAC GCCGCGACTT GAAGCTGATT
    GTCACGTCTG CGACGATGAA TGCAAAGCGG TTTTCAGACT TCTATGGTGG TGCACCCGAG
    TTTACCATTC CCGGAAGAAC CTTCCCTGTC GACATCATGT TCCATCGGTC ACCTGTCGAA
    GATTACGTGG ACCAGGCTGT GCAGCAAGTT CTGGCGATTC ACGTAGGAAA ACCAGCAGGC
    GATATCCTCG TTTTCATGAC CGGCCAGGAA GATATTGAGG TGACCTGCGA ACTCATCCGC
    GAGCGGCTCG ATGCCCTCAA CGACCCACCG AAACTCAGTA TCCTTCCCAT CTACAGTCAG
    ATGCCCGCAG ACTTGCAGGC CAAGATTTTC GACAGGGCTG CTCCGGGTGT CCGAAAGGTC
    ATCGTGGCCA CGAATATCGC GGAAACCAGT TTGACGGTGG ATGGTATCAT GTACGTGGTT
    GATGCAGGCT ATTCAAAGCT CAAGGTATAC AATCCACGAA TGGGTATGGA TACCCTTCAA
    ATTACGCCTA TTTCGCAAGC AAACGCCATG CAGCGCGCTG GTCGTGCGGG TCGCACTGGG
    CCTGGCCAAG CATATCATCT CTTCACCGAG AAGGCCTTCA AAGAGGAGAT GTACATGCAA
    ACCATTCCGG AGATTCAGAG AACGAATCTT AGCAATACCG TTCTACTCCT CAAGTCACTC
    GGTGTTAAAG ACCTCCTTGA TTTCGACTTT ATGGATCCAC CACCTCAGGA TACCATCAGT
    ACATCTCTCT TCGATTTGTG GGCGTTGGGA GCACTGGACA ACCTAGGTGA GCTCACCGAC
    TTGGGGCGGA AGATGAATGC GTTCCCGATG GACCCACCCC TTGCAAAACT GCTCATCACC
    TCGGAGGAGT ATGGGTGCAG CGAGGAAATG GTCACCATCG TTTCGATGCT TTCCGTTCCC
    AACGTATTCT ACCGGCCCAA AGAACGACAA GAGGAGTCAG ATGCGGCTCG CGAGAAGTTC
    TTTGTACCAG AATCAGACCA CTTGACCTAT CTGCACGTTT ACACACAATG GAAGGCCAAC
    GGGTATAACG ACGGTTGGTG CGTGCGACAC TTTTTGCATT CCAAGTCTCT CCGTCGTGCC
    AAGGAAGTCC GCGAGCAGCT CCTGGACATC ATGAAGATGC AGAATATGAA GATGACGAGT
    TGTGGCACCG ACTGGGATGT TATTCGCAAG TGCATTTGCT CTGGCTACTA TCACCAGGCA
    GCAAAGGTCA AGGGCATTGG GGAGTATATC AACTTGAGGA CCAGTGTCAC CGTCCAATTG
    CATCCGACAA GTGCGCTTTA TGGACTGGGC TTCTTGCCAG ACTACGTGGT GTACCACGAG
    CTGATCCTCA CAAGTAAAGA GTACATGTCG ACGGTGACCT CGGTAGACCC CCACTGGTTG
    GCGGATCTCG GTGGTGTATT TTATTCGGTC AAGGAGAAAG GATATTCGGC GCGGGAGAAG
    AGGATCACAG AGACGGAGTT CAACAGGAGA ATGGAGATTG AGGCGCGGAT GGCAGCGGAC
    AAGAAGCGGC AAGAGGATCA GCTGCACGAA GAAGAGCTTC AGCTTGAGAA GAAGGCGGCC
    CCGTCCAAGG ACAAGAAGGT GGTTAAAGCG GGTGCCGTGG TGAAGCCAGT CAGGAAACGT
    GCTGGAAGGG GATTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B