l(1)G0007 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	l(1)G0007
Entrez Gene ID	32373
Full Name	lethal (1) G0007
Synonyms	CG1375,CG1405,CG32604,Dmel\CG32604,EP764,EP784,Prp16,cg1405,l(1)G0028,l(1)G0103,l(1)G0176,l(1)G0308,l(1)G0416,l(1)G0476,l(1)G0491,prp16
General protein information	Preferred Names lethal (1) G0007 Names CG32604 gene product from transcript CG32604-RA CG32604-PA CG32604-PB l(1)G0007-PA l(1)G0007-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12E3-12E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_167396.2	NP_727764.1	lethal (1) G0007, isoform B
NM_132719.3	NP_572947.1	lethal (1) G0007, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DHX38	XP_511101.3
Bos taurus (cattle)	DHX38	NP_001069266.1
Mus musculus (house mouse)	Dhx38	NP_848467.1
Danio rerio (zebrafish)	dhx38	NP_957170.1
Saccharomyces cerevisiae (baker's yeast)	PRP16	NP_013012.3
Kluyveromyces lactis	KLLA0A07733g	XP_451344.1
Eremothecium gossypii	AGOS_ADR224W	NP_984320.1
Oryza sativa (rice)	Os07g0508000	NP_001059742.1
Xenopus tropicalis (tropical clawed frog)	dhx38	XP_002937626.2
	DHX38	XP_001097681.2
Canis lupus familiaris (dog)	DHX38	XP_005620809.1
Gallus gallus (chicken)	DHX38	NP_001025753.1
Caenorhabditis elegans (roundworm)	mog-1	NP_499212.1
Schizosaccharomyces pombe (fission yeast)	prp16	NP_595890.2
Rattus norvegicus (Norway rat)	Dhx38	NP_001099655.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008528	XP_316912.4
Homo sapiens (human)	DHX38	NP_054722.2
Drosophila melanogaster (fruit fly)	l(1)G0007	NP_572947.1
Magnaporthe oryzae (rice blast fungus)	MGG_11351	XP_003713740.1
Neurospora crassa	NCU02728	XP_965113.1
Arabidopsis thaliana (thale cress)	EMB3011	NP_196805.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following l(1)G0007 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(1)G0007 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01579	NM_167396.2 Latest version!	Drosophila melanogaster lethal (1) G0007 (l(1)G0007), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01578	NM_132719.3 Latest version!	Drosophila melanogaster lethal (1) G0007 (l(1)G0007), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

l(1)G0007 ( NM_167396.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727764.1 Drosophila melanogaster lethal (1) G0007 (l(1)G0007), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01579
Clone ID Related Accession (Same CDS sequence)	NM_167396.2
Accession Version	NM_167396.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (1) G0007, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167396.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTCGA GCAAGTTTGC CACCTTCTTC GGCAACGTGC CCACGTTCAC CATTCCGGGA 
CGCACCTTTC CCGTGGACGT GATGTTCAGC AAGAACACCT GCGAGGATTA CGTGGAATCG 
GCGGTGAAGC AAGCGTTGCA GGTTCACCTC ACGCCCAACG AGGGCGACAT GCTCATCTTC 
ATGCCCGGCC AGGAGGACAT CGAGGTGACG TGCGAGGTGC TCGAGGAGCG ACTGGCGGAG 
ATCGATAATG CGCCGGCACT AAGCATACTG CCCATCTATT CGCAATTGCC GTCCGATTTG 
CAAGCAAAGA TCTTTCAGAA ATCCAGCGAT GGATTGCGCA AGTGTGTGGT GGCCACGAAC 
ATAGCGGAAA CATCGCTAAC CGTCGACGGC ATCATCTACG TTATCGATTC CGGCTACTGC 
AAACTGAAGG TCTACAATCC GCGCATCGGC ATGGACGCCC TGCAGATCTA TCCCATATCG 
CAGGCGAATG CCAATCAGCG CAGCGGTCGT GCCGGACGCA CAGGACCCGG TCAGGCGTAC 
CGCTTGTATA CGCAGCGCCA GTACAAGGAC GAGCTGTTGG CCCTAACCGT GCCCGAGATT 
CAGCGGACCA ATCTGGCCAA TACGGTGCTG TTGCTCAAGT CACTGGGCGT CGTCGATCTG 
CTGCAATTCC ACTTTATGGA TCCACCGCCG CAGGATAACA TTCTTAACTC GCTGTACCAG 
TTGTGGATCC TGGGAGCACT GGACCACACT GGTGCGCTGA CCACACTGGG TCGCCAGATG 
GCCGAATTCC CGTTGGATCC GCCCCAGTGC CAGATGCTAA TCGTGGCCTG CCGCATGGGC 
TGCAGTGCCG AGGTGCTGAT CATAGTTTCC ATGTTGTCCG TGCCATCGAT CTTCTACCGG 
CCAAAGGGCC GCGAGGATGA GGCGGATGGT GTGCGCGAAA AGTTCCAAAG GCCCGAGTCC 
GATCACCTGA CCTACCTCAA CGTTTATCAG CAGTGGCGCC AGAACAATTA CAGTTCGACC 
TGGTGCAACG AACATTTTAT CCACATCAAG GCGATGCGGA AGGTGCGCGA GGTGCGGCAG 
CAGCTTAAGG ACATCATGAC GCAACAGAAT CTGAGCGTTA TAAGCTGCGG CATCGACTGG 
GACATTGTGA GGAAGTGCAT CTGCTCGGCA TACTTCTACC AGGCGGCCAG GCTCAAGGGT 
ATCGGAGAGT ATGTCAATCT GCGAACTGGC ATGCCATGCC ACCTGCATCC CACGTCCGCG 
CTGTATGGCT TGGGCACCAC GCCCGACTAT GTCGTCTATC ACGAGCTGAT CATGACCGCC 
AAGGAGTATA TGCAGTGCGC CACCGCTGTC GATGGCTACT GGCTGGCGGA ATTGGGTCCC 
ATGTTCTTTT CGGTTAAGGA GTCCGGTCGC AGCGGGCGCG AGAAGAAGAA ACAGGCGGCC 
GAGCATCTCA AGGAGATGGA GGAGCAGATG CTGAAGGCGC AGCACGAGAT GGAGGAGCGC 
AAGCAGCAGG CCGCCGAACG GGAGGAACAG CTGGCCACCA AGCAGGAGAT CGCCACACCG 
GGCAATGCCA CGCCCCGACG CACGCCGGCC AGGATTGGCC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727764.1
CDS	232..1836
Translation	MDSSKFATFFGNVPTFTIPGRTFPVDVMFSKNTCEDYVESAVKQALQVHLTPNEGDMLIFMPGQEDIEVTCEVLEERLAEIDNAPALSILPIYSQLPSDLQAKIFQKSSDGLRKCVVATNIAETSLTVDGIIYVIDSGYCKLKVYNPRIGMDALQIYPISQANANQRSGRAGRTGPGQAYRLYTQRQYKDELLALTVPEIQRTNLANTVLLLKSLGVVDLLQFHFMDPPPQDNILNSLYQLWILGALDHTGALTTLGRQMAEFPLDPPQCQMLIVACRMGCSAEVLIIVSMLSVPSIFYRPKGREDEADGVREKFQRPESDHLTYLNVYQQWRQNNYSSTWCNEHFIHIKAMRKVREVRQQLKDIMTQQNLSVISCGIDWDIVRKCICSAYFYQAARLKGIGEYVNLRTGMPCHLHPTSALYGLGTTPDYVVYHELIMTAKEYMQCATAVDGYWLAELGPMFFSVKESGRSGREKKKQAAEHLKEMEEQMLKAQHEMEERKQQAAEREEQLATKQEIATPGNATPRRTPARIGL

Target ORF information:

RefSeq Version	NM_167396.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (1) G0007 (l(1)G0007), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167396.2

ORF Insert Sequence:

ATGGACTCGA GCAAGTTTGC CACCTTCTTC GGCAACGTGC CCACGTTCAC CATTCCGGGA 
CGCACCTTTC CCGTGGACGT GATGTTCAGC AAGAACACCT GCGAGGATTA CGTGGAATCG 
GCGGTGAAGC AAGCGTTGCA GGTTCACCTC ACGCCCAACG AGGGCGACAT GCTCATCTTC 
ATGCCCGGCC AGGAGGACAT CGAGGTGACG TGCGAGGTGC TCGAGGAGCG ACTGGCGGAG 
ATCGATAATG CGCCGGCACT AAGCATACTG CCCATCTATT CGCAATTGCC GTCCGATTTG 
CAAGCAAAGA TCTTTCAGAA ATCCAGCGAT GGATTGCGCA AGTGTGTGGT GGCCACGAAC 
ATAGCGGAAA CATCGCTAAC CGTCGACGGC ATCATCTACG TTATCGATTC CGGCTACTGC 
AAACTGAAGG TCTACAATCC GCGCATCGGC ATGGACGCCC TGCAGATCTA TCCCATATCG 
CAGGCGAATG CCAATCAGCG CAGCGGTCGT GCCGGACGCA CAGGACCCGG TCAGGCGTAC 
CGCTTGTATA CGCAGCGCCA GTACAAGGAC GAGCTGTTGG CCCTAACCGT GCCCGAGATT 
CAGCGGACCA ATCTGGCCAA TACGGTGCTG TTGCTCAAGT CACTGGGCGT CGTCGATCTG 
CTGCAATTCC ACTTTATGGA TCCACCGCCG CAGGATAACA TTCTTAACTC GCTGTACCAG 
TTGTGGATCC TGGGAGCACT GGACCACACT GGTGCGCTGA CCACACTGGG TCGCCAGATG 
GCCGAATTCC CGTTGGATCC GCCCCAGTGC CAGATGCTAA TCGTGGCCTG CCGCATGGGC 
TGCAGTGCCG AGGTGCTGAT CATAGTTTCC ATGTTGTCCG TGCCATCGAT CTTCTACCGG 
CCAAAGGGCC GCGAGGATGA GGCGGATGGT GTGCGCGAAA AGTTCCAAAG GCCCGAGTCC 
GATCACCTGA CCTACCTCAA CGTTTATCAG CAGTGGCGCC AGAACAATTA CAGTTCGACC 
TGGTGCAACG AACATTTTAT CCACATCAAG GCGATGCGGA AGGTGCGCGA GGTGCGGCAG 
CAGCTTAAGG ACATCATGAC GCAACAGAAT CTGAGCGTTA TAAGCTGCGG CATCGACTGG 
GACATTGTGA GGAAGTGCAT CTGCTCGGCA TACTTCTACC AGGCGGCCAG GCTCAAGGGT 
ATCGGAGAGT ATGTCAATCT GCGAACTGGC ATGCCATGCC ACCTGCATCC CACGTCCGCG 
CTGTATGGCT TGGGCACCAC GCCCGACTAT GTCGTCTATC ACGAGCTGAT CATGACCGCC 
AAGGAGTATA TGCAGTGCGC CACCGCTGTC GATGGCTACT GGCTGGCGGA ATTGGGTCCC 
ATGTTCTTTT CGGTTAAGGA GTCCGGTCGC AGCGGGCGCG AGAAGAAGAA ACAGGCGGCC 
GAGCATCTCA AGGAGATGGA GGAGCAGATG CTGAAGGCGC AGCACGAGAT GGAGGAGCGC 
AAGCAGCAGG CCGCCGAACG GGAGGAACAG CTGGCCACCA AGCAGGAGAT CGCCACACCG 
GGCAATGCCA CGCCCCGACG CACGCCGGCC AGGATTGGCC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

l(1)G0007 ( NM_132719.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572947.1 Drosophila melanogaster lethal (1) G0007 (l(1)G0007), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01578
Clone ID Related Accession (Same CDS sequence)	NM_132719.3
Accession Version	NM_132719.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (1) G0007, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132719.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGACG ACGACGAGTC GGGTGTCCAT CGGCTGGAGG GCACCGCTGG CCAGGAGACG 
CGCGGCGGCC TGGTCATCCG GAAGCCCAAG GATGCCGGCG CTGGCGGTGG CGGTGGATTC 
AAGGTGCCGC AGGGATCGAT GTTGGGCCTG GACAAGCTGG CGGCCAAGCG ACGGGCCGAA 
AAGGAGCGAT CCGAGCGACT GATCTCCTTC AAGGACAGCG AATTCGATGA CACCGGCGGC 
GGGAGCTCCA CGCCGCAGGC CAACGCCTCT GGCGCTTCCA GTGAGTTTGC CTTCAAGAAA 
CCGGACACCA AGAGCTTCGA AAAGCTGCGC GGCCAGCTGC GCGAGCACAA AGACGATACA 
CCCTCGCACA CCGGCGGCGT TTCCGAGAAG GCACGCGAGC GTCTGCGCGA GCACATCCAG 
CGGGACAGAA AACGTGGCCC AGTCAGCAGC ACAGCGGCAG AGGGCAGGGA CAGGGATCGC 
GACTGGGATC GTGACCGACG CAGGGACCGG GACAGAGATC GGGATCGTGA CCGCGACCGG 
CGCCAACACA GAGAAAGGGA GCGCGATCGG CACCGCAGCT GGGATCGGGA TCGTGGTCGT 
GATCGTGATC GTGATCGCTC CATGTCCGAG CGCAGTGTCC ATACGCCGCG TGAGCCGGGA 
ACACCAGGCG GCAGCAGCGG TGGCATATCC AATAGCTCGT GGGACGACGA GGACGGCGAG 
TTTGGACAGC GCAAGTCGGA CTGGGATATG CCAACGCCCA GACGCCATGG CAACAAGTCG 
GGTGATTGGT CAGTGCGGAG CGGCGGCAGT CGGCGTAATC ACGGACGCCA GGATGACACT 
GTCCGGCCAA CGCCAGCGCA TCGCTACAAT CAGTGGGCAC ACGGTCGCAA GCGAAGCGGC 
GCAACGCCAT GGGGCGAGGA TCCCGAATCG CTGGATCTGT GGGAGGAGGA GCAGCGTCGC 
CTCGACCGCG AGTGGTACAA CATTGACGAG GGCTACGACG ATGAGAACAA TCCATTCGGT 
GGCCCCAACT CCGAGTACTT TCGCAAGCGC GAAGAGCAGC TGGAGCAGAA GAGAACAAAG 
AGAATCAGCG CTCAGCAGCG CCAGAATAAC CGTGACAACG AGTTGTGGGA GCGCAATCGT 
ATGCTCACCT CGGGCGTGGT CACATTGATC AGCGTCAACG ACGATTTCGA TGAGGAGGCG 
CTGGAGCGAG TGCATCTCCT GGTGCATCAC ATAATACCGC CATTTCTCGA CGGCCGCATC 
GTGTTCACCA AGCAACCGGA GCCGGTGGTG CCCGTCAAGG ATCCCACCTC GGATATGGCA 
CTGCTGGCGC GCAAGGGCAG CGCCCTGGTG CGCAACTATC GCGAGCAAAA GGAGCGGCGC 
AAGGCGCAAA AGAAGCACTG GGAGCTGAGC GGCACCAAGC TGGGCAACAT TATGGGTGTA 
CAGCGGCCGC AGGACGAGGA CGATATGCGT TTCGACAAGG AGAAGGACAA GGCCGACTAT 
CGCAAGGATC AGAAGTTCGC CGACCATATG CGCGACCAGG ATACGGGTGG CAAGAGCGAT 
TTCTCGCGCA AAAAGACCAT CTCGGAGCAG CGCCGCTTCC TGCCCGTTTT CGCATCGCGT 
CAAGAGTTGC TCAACGTTAT TCGCGAGAAC TCGGTGATCA TTATCGTGGG CGAGACGGGC 
AGCGGCAAGA CGACACAGCT AACCCAGTAT CTTCACGAGG ATGGCTACAG CAAGCGTGGC 
ATGATCGGTT GCACGCAGCC GCGTCGTGTG GCCGCCATGT CGGTGGCCAA GCGAGTCTCC 
GACGAGATGG ACACCCAGCT GGGCGAGGAC GTGGGCTATG CCATCCGTTT CGAGGACTGC 
ACGTCGGAGC GCACGGTCAT CAAGTACATG ACGGATGGTA TTCTGCTCCG CGAGAGTCTG 
CGTGATCCGG AACTAGACAG CTACTCCGCC ATCATTATGG ACGAGGCCCA CGAGCGATCC 
CTTTCGACGG ACGTGCTCTT TGGATTGCTG CGCGAGATTG TGGCCCGGCG ACATGACCTG 
AAGCTGATCG TGACCTCCGC CACGATGGAC TCGAGCAAGT TTGCCACCTT CTTCGGCAAC 
GTGCCCACGT TCACCATTCC GGGACGCACC TTTCCCGTGG ACGTGATGTT CAGCAAGAAC 
ACCTGCGAGG ATTACGTGGA ATCGGCGGTG AAGCAAGCGT TGCAGGTTCA CCTCACGCCC 
AACGAGGGCG ACATGCTCAT CTTCATGCCC GGCCAGGAGG ACATCGAGGT GACGTGCGAG 
GTGCTCGAGG AGCGACTGGC GGAGATCGAT AATGCGCCGG CACTAAGCAT ACTGCCCATC 
TATTCGCAAT TGCCGTCCGA TTTGCAAGCA AAGATCTTTC AGAAATCCAG CGATGGATTG 
CGCAAGTGTG TGGTGGCCAC GAACATAGCG GAAACATCGC TAACCGTCGA CGGCATCATC 
TACGTTATCG ATTCCGGCTA CTGCAAACTG AAGGTCTACA ATCCGCGCAT CGGCATGGAC 
GCCCTGCAGA TCTATCCCAT ATCGCAGGCG AATGCCAATC AGCGCAGCGG TCGTGCCGGA 
CGCACAGGAC CCGGTCAGGC GTACCGCTTG TATACGCAGC GCCAGTACAA GGACGAGCTG 
TTGGCCCTAA CCGTGCCCGA GATTCAGCGG ACCAATCTGG CCAATACGGT GCTGTTGCTC 
AAGTCACTGG GCGTCGTCGA TCTGCTGCAA TTCCACTTTA TGGATCCACC GCCGCAGGAT 
AACATTCTTA ACTCGCTGTA CCAGTTGTGG ATCCTGGGAG CACTGGACCA CACTGGTGCG 
CTGACCACAC TGGGTCGCCA GATGGCCGAA TTCCCGTTGG ATCCGCCCCA GTGCCAGATG 
CTAATCGTGG CCTGCCGCAT GGGCTGCAGT GCCGAGGTGC TGATCATAGT TTCCATGTTG 
TCCGTGCCAT CGATCTTCTA CCGGCCAAAG GGCCGCGAGG ATGAGGCGGA TGGTGTGCGC 
GAAAAGTTCC AAAGGCCCGA GTCCGATCAC CTGACCTACC TCAACGTTTA TCAGCAGTGG 
CGCCAGAACA ATTACAGTTC GACCTGGTGC AACGAACATT TTATCCACAT CAAGGCGATG 
CGGAAGGTGC GCGAGGTGCG GCAGCAGCTT AAGGACATCA TGACGCAACA GAATCTGAGC 
GTTATAAGCT GCGGCATCGA CTGGGACATT GTGAGGAAGT GCATCTGCTC GGCATACTTC 
TACCAGGCGG CCAGGCTCAA GGGTATCGGA GAGTATGTCA ATCTGCGAAC TGGCATGCCA 
TGCCACCTGC ATCCCACGTC CGCGCTGTAT GGCTTGGGCA CCACGCCCGA CTATGTCGTC 
TATCACGAGC TGATCATGAC CGCCAAGGAG TATATGCAGT GCGCCACCGC TGTCGATGGC 
TACTGGCTGG CGGAATTGGG TCCCATGTTC TTTTCGGTTA AGGAGTCCGG TCGCAGCGGG 
CGCGAGAAGA AGAAACAGGC GGCCGAGCAT CTCAAGGAGA TGGAGGAGCA GATGCTGAAG 
GCGCAGCACG AGATGGAGGA GCGCAAGCAG CAGGCCGCCG AACGGGAGGA ACAGCTGGCC 
ACCAAGCAGG AGATCGCCAC ACCGGGCAAT GCCACGCCCC GACGCACGCC GGCCAGGATT 
GGCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572947.1
CDS	102..3770
Translation	MSDDDESGVHRLEGTAGQETRGGLVIRKPKDAGAGGGGGFKVPQGSMLGLDKLAAKRRAEKERSERLISFKDSEFDDTGGGSSTPQANASGASSEFAFKKPDTKSFEKLRGQLREHKDDTPSHTGGVSEKARERLREHIQRDRKRGPVSSTAAEGRDRDRDWDRDRRRDRDRDRDRDRDRRQHRERERDRHRSWDRDRGRDRDRDRSMSERSVHTPREPGTPGGSSGGISNSSWDDEDGEFGQRKSDWDMPTPRRHGNKSGDWSVRSGGSRRNHGRQDDTVRPTPAHRYNQWAHGRKRSGATPWGEDPESLDLWEEEQRRLDREWYNIDEGYDDENNPFGGPNSEYFRKREEQLEQKRTKRISAQQRQNNRDNELWERNRMLTSGVVTLISVNDDFDEEALERVHLLVHHIIPPFLDGRIVFTKQPEPVVPVKDPTSDMALLARKGSALVRNYREQKERRKAQKKHWELSGTKLGNIMGVQRPQDEDDMRFDKEKDKADYRKDQKFADHMRDQDTGGKSDFSRKKTISEQRRFLPVFASRQELLNVIRENSVIIIVGETGSGKTTQLTQYLHEDGYSKRGMIGCTQPRRVAAMSVAKRVSDEMDTQLGEDVGYAIRFEDCTSERTVIKYMTDGILLRESLRDPELDSYSAIIMDEAHERSLSTDVLFGLLREIVARRHDLKLIVTSATMDSSKFATFFGNVPTFTIPGRTFPVDVMFSKNTCEDYVESAVKQALQVHLTPNEGDMLIFMPGQEDIEVTCEVLEERLAEIDNAPALSILPIYSQLPSDLQAKIFQKSSDGLRKCVVATNIAETSLTVDGIIYVIDSGYCKLKVYNPRIGMDALQIYPISQANANQRSGRAGRTGPGQAYRLYTQRQYKDELLALTVPEIQRTNLANTVLLLKSLGVVDLLQFHFMDPPPQDNILNSLYQLWILGALDHTGALTTLGRQMAEFPLDPPQCQMLIVACRMGCSAEVLIIVSMLSVPSIFYRPKGREDEADGVREKFQRPESDHLTYLNVYQQWRQNNYSSTWCNEHFIHIKAMRKVREVRQQLKDIMTQQNLSVISCGIDWDIVRKCICSAYFYQAARLKGIGEYVNLRTGMPCHLHPTSALYGLGTTPDYVVYHELIMTAKEYMQCATAVDGYWLAELGPMFFSVKESGRSGREKKKQAAEHLKEMEEQMLKAQHEMEERKQQAAEREEQLATKQEIATPGNATPRRTPARIGL

Target ORF information:

RefSeq Version	NM_132719.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (1) G0007 (l(1)G0007), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132719.3

ORF Insert Sequence:

ATGTCCGACG ACGACGAGTC GGGTGTCCAT CGGCTGGAGG GCACCGCTGG CCAGGAGACG 
CGCGGCGGCC TGGTCATCCG GAAGCCCAAG GATGCCGGCG CTGGCGGTGG CGGTGGATTC 
AAGGTGCCGC AGGGATCGAT GTTGGGCCTG GACAAGCTGG CGGCCAAGCG ACGGGCCGAA 
AAGGAGCGAT CCGAGCGACT GATCTCCTTC AAGGACAGCG AATTCGATGA CACCGGCGGC 
GGGAGCTCCA CGCCGCAGGC CAACGCCTCT GGCGCTTCCA GTGAGTTTGC CTTCAAGAAA 
CCGGACACCA AGAGCTTCGA AAAGCTGCGC GGCCAGCTGC GCGAGCACAA AGACGATACA 
CCCTCGCACA CCGGCGGCGT TTCCGAGAAG GCACGCGAGC GTCTGCGCGA GCACATCCAG 
CGGGACAGAA AACGTGGCCC AGTCAGCAGC ACAGCGGCAG AGGGCAGGGA CAGGGATCGC 
GACTGGGATC GTGACCGACG CAGGGACCGG GACAGAGATC GGGATCGTGA CCGCGACCGG 
CGCCAACACA GAGAAAGGGA GCGCGATCGG CACCGCAGCT GGGATCGGGA TCGTGGTCGT 
GATCGTGATC GTGATCGCTC CATGTCCGAG CGCAGTGTCC ATACGCCGCG TGAGCCGGGA 
ACACCAGGCG GCAGCAGCGG TGGCATATCC AATAGCTCGT GGGACGACGA GGACGGCGAG 
TTTGGACAGC GCAAGTCGGA CTGGGATATG CCAACGCCCA GACGCCATGG CAACAAGTCG 
GGTGATTGGT CAGTGCGGAG CGGCGGCAGT CGGCGTAATC ACGGACGCCA GGATGACACT 
GTCCGGCCAA CGCCAGCGCA TCGCTACAAT CAGTGGGCAC ACGGTCGCAA GCGAAGCGGC 
GCAACGCCAT GGGGCGAGGA TCCCGAATCG CTGGATCTGT GGGAGGAGGA GCAGCGTCGC 
CTCGACCGCG AGTGGTACAA CATTGACGAG GGCTACGACG ATGAGAACAA TCCATTCGGT 
GGCCCCAACT CCGAGTACTT TCGCAAGCGC GAAGAGCAGC TGGAGCAGAA GAGAACAAAG 
AGAATCAGCG CTCAGCAGCG CCAGAATAAC CGTGACAACG AGTTGTGGGA GCGCAATCGT 
ATGCTCACCT CGGGCGTGGT CACATTGATC AGCGTCAACG ACGATTTCGA TGAGGAGGCG 
CTGGAGCGAG TGCATCTCCT GGTGCATCAC ATAATACCGC CATTTCTCGA CGGCCGCATC 
GTGTTCACCA AGCAACCGGA GCCGGTGGTG CCCGTCAAGG ATCCCACCTC GGATATGGCA 
CTGCTGGCGC GCAAGGGCAG CGCCCTGGTG CGCAACTATC GCGAGCAAAA GGAGCGGCGC 
AAGGCGCAAA AGAAGCACTG GGAGCTGAGC GGCACCAAGC TGGGCAACAT TATGGGTGTA 
CAGCGGCCGC AGGACGAGGA CGATATGCGT TTCGACAAGG AGAAGGACAA GGCCGACTAT 
CGCAAGGATC AGAAGTTCGC CGACCATATG CGCGACCAGG ATACGGGTGG CAAGAGCGAT 
TTCTCGCGCA AAAAGACCAT CTCGGAGCAG CGCCGCTTCC TGCCCGTTTT CGCATCGCGT 
CAAGAGTTGC TCAACGTTAT TCGCGAGAAC TCGGTGATCA TTATCGTGGG CGAGACGGGC 
AGCGGCAAGA CGACACAGCT AACCCAGTAT CTTCACGAGG ATGGCTACAG CAAGCGTGGC 
ATGATCGGTT GCACGCAGCC GCGTCGTGTG GCCGCCATGT CGGTGGCCAA GCGAGTCTCC 
GACGAGATGG ACACCCAGCT GGGCGAGGAC GTGGGCTATG CCATCCGTTT CGAGGACTGC 
ACGTCGGAGC GCACGGTCAT CAAGTACATG ACGGATGGTA TTCTGCTCCG CGAGAGTCTG 
CGTGATCCGG AACTAGACAG CTACTCCGCC ATCATTATGG ACGAGGCCCA CGAGCGATCC 
CTTTCGACGG ACGTGCTCTT TGGATTGCTG CGCGAGATTG TGGCCCGGCG ACATGACCTG 
AAGCTGATCG TGACCTCCGC CACGATGGAC TCGAGCAAGT TTGCCACCTT CTTCGGCAAC 
GTGCCCACGT TCACCATTCC GGGACGCACC TTTCCCGTGG ACGTGATGTT CAGCAAGAAC 
ACCTGCGAGG ATTACGTGGA ATCGGCGGTG AAGCAAGCGT TGCAGGTTCA CCTCACGCCC 
AACGAGGGCG ACATGCTCAT CTTCATGCCC GGCCAGGAGG ACATCGAGGT GACGTGCGAG 
GTGCTCGAGG AGCGACTGGC GGAGATCGAT AATGCGCCGG CACTAAGCAT ACTGCCCATC 
TATTCGCAAT TGCCGTCCGA TTTGCAAGCA AAGATCTTTC AGAAATCCAG CGATGGATTG 
CGCAAGTGTG TGGTGGCCAC GAACATAGCG GAAACATCGC TAACCGTCGA CGGCATCATC 
TACGTTATCG ATTCCGGCTA CTGCAAACTG AAGGTCTACA ATCCGCGCAT CGGCATGGAC 
GCCCTGCAGA TCTATCCCAT ATCGCAGGCG AATGCCAATC AGCGCAGCGG TCGTGCCGGA 
CGCACAGGAC CCGGTCAGGC GTACCGCTTG TATACGCAGC GCCAGTACAA GGACGAGCTG 
TTGGCCCTAA CCGTGCCCGA GATTCAGCGG ACCAATCTGG CCAATACGGT GCTGTTGCTC 
AAGTCACTGG GCGTCGTCGA TCTGCTGCAA TTCCACTTTA TGGATCCACC GCCGCAGGAT 
AACATTCTTA ACTCGCTGTA CCAGTTGTGG ATCCTGGGAG CACTGGACCA CACTGGTGCG 
CTGACCACAC TGGGTCGCCA GATGGCCGAA TTCCCGTTGG ATCCGCCCCA GTGCCAGATG 
CTAATCGTGG CCTGCCGCAT GGGCTGCAGT GCCGAGGTGC TGATCATAGT TTCCATGTTG 
TCCGTGCCAT CGATCTTCTA CCGGCCAAAG GGCCGCGAGG ATGAGGCGGA TGGTGTGCGC 
GAAAAGTTCC AAAGGCCCGA GTCCGATCAC CTGACCTACC TCAACGTTTA TCAGCAGTGG 
CGCCAGAACA ATTACAGTTC GACCTGGTGC AACGAACATT TTATCCACAT CAAGGCGATG 
CGGAAGGTGC GCGAGGTGCG GCAGCAGCTT AAGGACATCA TGACGCAACA GAATCTGAGC 
GTTATAAGCT GCGGCATCGA CTGGGACATT GTGAGGAAGT GCATCTGCTC GGCATACTTC 
TACCAGGCGG CCAGGCTCAA GGGTATCGGA GAGTATGTCA ATCTGCGAAC TGGCATGCCA 
TGCCACCTGC ATCCCACGTC CGCGCTGTAT GGCTTGGGCA CCACGCCCGA CTATGTCGTC 
TATCACGAGC TGATCATGAC CGCCAAGGAG TATATGCAGT GCGCCACCGC TGTCGATGGC 
TACTGGCTGG CGGAATTGGG TCCCATGTTC TTTTCGGTTA AGGAGTCCGG TCGCAGCGGG 
CGCGAGAAGA AGAAACAGGC GGCCGAGCAT CTCAAGGAGA TGGAGGAGCA GATGCTGAAG 
GCGCAGCACG AGATGGAGGA GCGCAAGCAG CAGGCCGCCG AACGGGAGGA ACAGCTGGCC 
ACCAAGCAGG AGATCGCCAC ACCGGGCAAT GCCACGCCCC GACGCACGCC GGCCAGGATT 
GGCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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l(1)G0007 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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