prp16 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	prp16
Entrez Gene ID	2540006
Full Name	ATP-dependent RNA helicase Prp16 (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021796.3	NP_595890.2	putative ATP-dependent RNA helicase Prp16

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DHX38	XP_511101.3
Bos taurus (cattle)	DHX38	NP_001069266.1
Mus musculus (house mouse)	Dhx38	NP_848467.1
Danio rerio (zebrafish)	dhx38	NP_957170.1
Saccharomyces cerevisiae (baker's yeast)	PRP16	NP_013012.3
Kluyveromyces lactis	KLLA0A07733g	XP_451344.1
Eremothecium gossypii	AGOS_ADR224W	NP_984320.1
Oryza sativa (rice)	Os07g0508000	NP_001059742.1
Xenopus tropicalis (tropical clawed frog)	dhx38	XP_002937626.2
	DHX38	XP_001097681.2
Canis lupus familiaris (dog)	DHX38	XP_005620809.1
Gallus gallus (chicken)	DHX38	NP_001025753.1
Caenorhabditis elegans (roundworm)	mog-1	NP_499212.1
Schizosaccharomyces pombe (fission yeast)	prp16	NP_595890.2
Rattus norvegicus (Norway rat)	Dhx38	NP_001099655.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008528	XP_316912.4
Homo sapiens (human)	DHX38	NP_054722.2
Drosophila melanogaster (fruit fly)	l(1)G0007	NP_572947.1
Magnaporthe oryzae (rice blast fungus)	MGG_11351	XP_003713740.1
Neurospora crassa	NCU02728	XP_965113.1
Arabidopsis thaliana (thale cress)	EMB3011	NP_196805.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

n, we demonstrate that the splicing factor spPrp16p is enriched at the centromere, and associates with Cid12p (a factor in the RNAi pathway) and the intron-containing dg ncRNA. Interestingly, removal of the dg intron, mutations of its splice sites, or replacement of the dg intron with an euchromatic intron significantly decreased H3K9 dimethylation
Title: The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin.

The following prp16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prp16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100032	NM_001021796.3 Latest version!	Schizosaccharomyces pombe putative ATP-dependent RNA helicase Prp16 (prp16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prp16 ( NM_001021796.3 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595890.2 Schizosaccharomyces pombe putative ATP-dependent RNA helicase Prp16 (prp16), mRNA.

Price & Availability↑

CloneID	OSc100032
Clone ID Related Accession (Same CDS sequence)	NM_001021796.3
Accession Version	NM_001021796.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative ATP-dependent RNA helicase Prp16
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021796.2.

ATGCTTTCAC ATAATCAAAA TGATTTGCTA TTTACGAAGT TAATAGATAA ATTAACTGAT 
TATATGTCCA GTAAAGATGC AGCTACATCG CTTGCCAGTC GAGTTTTGAC AATTGCCAAG 
GGTAGCTCGT CCAGCACCGA GTTTTCTAAT GCATTACATA CATTTGGACG ATTTTCCGAT 
AATGACAGCG TCCTTATTTA CGATTTATGC AAATCTATAA TTGGTTTAGA GACAAATTTG 
GGGATTTCTA AAGATAAACT TCCTGGGAAT GATGGGTCTC AGGTAGCTGG ATTAGTTTTG 
TCTAATCGTT CTGGTTTGAA AGGCCGAGAA CCCAAAAAAT CCCAGCTTGG ATTGGATGTT 
CTTGCTGTTC AAAAAAAGAA AGAGAAATCT CAAGTAGAAG GGCGCTCTGA ATTATCCCAA 
GTAGAAAATG ATTCCTTGGA GTCTATAGGA AACTATGACA GGGTGGAATT TAAGAGACCT 
AAAAATCCAC ATGAAAAACA TTTTCGTCCC CTTCGTCAAC GGTCATCTGT TGACGATCAT 
CAAGAGTTTG AAAGTGAAGA TGATAAATAT CGTAGAAATT CTTATTCTAG CTGGTCTGGA 
AGTAATTTCG AGTCTTCCAA TGGATCTAAT CGTCGGCGGT ATCGAACTCA AATGGAAGAA 
CCTTTATCCT CAAAACGCAG AAATCGGTTT GGTAATGGTT CTCGAAGAGA CGTCGATAGC 
TCTAAATATT CGCACGATAG TGATTATTCT TATGGTGCAC ACAGCTCTTG GGATGCCCGC 
GACGTTGAGT ACCCCGAAGA AGATCCCGAG AGTAAAGCTG ATCGTCAACG TTGGGAAGAG 
GAGCAAGCTC ACTTGGATCG TGATTGGTAC ATGAATTCCG AGTCGCAAAA TTTACTTGGC 
GATGAAGTTC ACAATCCATT CTCCGATTTT GAAACTGTCG AAGATCGTGC ACATGAAGCT 
GAGTTTATCG AAAAGCAGAA AAAGCATTTG TCAATTGAAG CATCAGACAG ATTTAAAGAA 
AATTCTATGT GGGAAAAAAA TAGAATGATC ACATCTGGTG TTTCAAAAGC TCCAGGGCTC 
GAATCTGATT ACTCCCTTAT GGAAGAGAGG CGTGTTCACT TGCTTGTCGA TGAACTGCGT 
CCTCATTTTT TGGATGGGGC CGAATTTTCT TCTAAGAAAG TCGGTGATAT TACATCGGTT 
CGAGATCCAC AAAGCGACCT TGCAATAAAT GCTAGATTAG GATCTCGTCT TGTACGTGAA 
CGAAGAGAAT TTCGGGAGCG TCAAAAAGCA GCAAGTGCTG CTACATCCCT TGCTGGTACA 
TCTTTGGGTA ACGTGATGGG TCTCAAAGAT TCTAATGATG AAGATGCCAA GGCCGGTACC 
ACACCTGTTA AAGTAGCTGG ACGTTCTGAA CAGTCGAATA AGAAGGATAC TGAGTTCGCT 
CGCACGAAAT CATATAGAGA ACAAAGAGAG TTCCTACCTG CCTTTGCCGT GCGTGAACAG 
CTTCTTTCTG TTATACGTGA TAACCAAGTT CTTATTGTAG TTGGTGAGAC TGGTTCTGGT 
AAAACTACCC AATTAGCTCA ATTCTTATAT GAGGATGGCT ATCATAGAAA TGGCATGATA 
GGGTGTACTC AACCTCGTCG TGTCGCCGCC ATGTCAGTTG CAAAAAGGGT TAGTGAGGAA 
ATGGGTGTTC GTCTAGGTTC AACAGTTGGT TATTCAATTC GTTTCGAAGA TGTTACTGGA 
CCGGATACCG TAATTAAGTA CATGACTGAC GGTGTTTTAC TAAGAGAATC CTTGATGCAA 
AATAATCTAG AAAAATATAG CGTCATTATA ATGGATGAAG CACATGAGCG ATCCCTTAAT 
ACAGATATTT TAATGGGTTT ACTAAAAAAG GTGTTATCCA GGAGAAGAGA TATCAAGTTG 
CTCGTTACAT CCGCTACTAT GAATTCCCAG AAATTCTCTG ATTTTTTTGG AGGCGCCCCA 
CAGTTTACAA TTCCAGGTCG TACTTACCCG GTGGATATTA TGTTTGCTAA AGCCCCTTGC 
TCTGATTACG TTGAAGCAGC TGTACGACAG GTATTGCAGA TTCACCTCTC TCAGCCTGCA 
GGTGATATCT TAGTTTTTAT GACAGGGCAG GAAGATATTG AAGCTACATG CGAGATCATT 
GCAGATAGGT TAAATCAGTT GCATGATGCA CCACGCTTGT CCATTTTGCC TATCTACTCA 
CAAATGCCTG CGGATTTACA AGCAAAAATT TTTGATTCCG CCGAGCCAGG TGTTAGAAAG 
GTTGTGGTCG CAACTAATAT TGCGGAAACT TCGCTCACTG TGCACGGAAT ATCATATGTT 
GTAGATACTG GATATTGTAA ACTGAAAATG TATAATTCTA AGTTGGGTAT TGATACTCTG 
CAAGTTACAC CTATTTCGCA AGCTAATGCG AATCAAAGAG CCGGTCGTGC TGGGCGTACT 
GGACCCGGTA TTGCATATCG CCTTTATACC GAAATGGCTT ATATTCGTGA AATGTTTGAA 
ACGACCTTAC CTGAAATTCA GCGTACCAAT CTATCAAACA CAGTTCTTAT TTTAAAATCC 
CTTGGTGTTG AAGAAATTTC GGATTTTGAT TTCATGGACC GTCCTCCAAA TGATACGTTA 
ATGGCATCTC TGTATGAATT ATGGACTTTA GGAGCCCTCG ACAATTTTGG AAAACTTACT 
ACTTTAGGAA AGAAAATGTC GTTATTTCCA ATGGATCCTT CTCTTTCAAA ACTTATTATT 
ATTGCTGAAG ATTATAAATG TACTGAGGAA ATTATTACGA TTGTTTCCAT GCTTTCAGTT 
CCGTCTGTAT TTTATAGACC CAAGGAAAGA GCTGAAGAGA GTGACGCTGC AAGAGAAAAG 
TTTAATGTGC CAGAAAGTGA TCATTTAATG CTACTCAATA TTTATCAGCA TTGGCAAAGA 
AATGGATATT CAAATTCATG GTGCAGTAAA CATTTTTTAC ACTCGAAAAC TCTTAAACGG 
GCGCGCGATA TTCGTCAACA GCTGGTCGAA ATTATGTCAA AGCAAAAGAT TAGTTTAGAA 
TCTGTATCCG ATTGGGACAT TGTTCGGAGA GTTTTGTGTT CTGCGTACTT TCATCAAGCA 
GCATGCGCTA AAGGTATTGG GGAGTATGTT CATTTACGAA GCGGCATGCC TTGCCATCTA 
CATGTTACTT CGAGTCTTTA TGGCTTAGGG TATCTACCTG ATTATGTTAT ATATCACGAA 
CTTGTACTCA CTTCCAAGGA GTACATGAAT ATTGTCACCA GTGTTGATCC ATACTGGCTA 
GCTGAGTTTG GTGGAGTTTA CTACTCTGTG AAGGAAAGAT TCCGAAATGA AACTGAAAGC 
TATGATCGAG TTTTTTCTAG CAAACCTCAA TTGGATGCAC AGATAGCCGC CGACCGAGAA 
CTTGATGCAA AGCAAAAGCT TGCGAAGAAT CAAGAACCCG TGAAATCAAA ACGAAAGTCA 
GTGATACGTG CCAAACCTCC TAGAAGGGTT CGTGGATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595890.2
CDS	152..3673
Translation	MLSHNQNDLLFTKLIDKLTDYMSSKDAATSLASRVLTIAKGSSSSTEFSNALHTFGRFSDNDSVLIYDLCKSIIGLETNLGISKDKLPGNDGSQVAGLVLSNRSGLKGREPKKSQLGLDVLAVQKKKEKSQVEGRSELSQVENDSLESIGNYDRVEFKRPKNPHEKHFRPLRQRSSVDDHQEFESEDDKYRRNSYSSWSGSNFESSNGSNRRRYRTQMEEPLSSKRRNRFGNGSRRDVDSSKYSHDSDYSYGAHSSWDARDVEYPEEDPESKADRQRWEEEQAHLDRDWYMNSESQNLLGDEVHNPFSDFETVEDRAHEAEFIEKQKKHLSIEASDRFKENSMWEKNRMITSGVSKAPGLESDYSLMEERRVHLLVDELRPHFLDGAEFSSKKVGDITSVRDPQSDLAINARLGSRLVRERREFRERQKAASAATSLAGTSLGNVMGLKDSNDEDAKAGTTPVKVAGRSEQSNKKDTEFARTKSYREQREFLPAFAVREQLLSVIRDNQVLIVVGETGSGKTTQLAQFLYEDGYHRNGMIGCTQPRRVAAMSVAKRVSEEMGVRLGSTVGYSIRFEDVTGPDTVIKYMTDGVLLRESLMQNNLEKYSVIIMDEAHERSLNTDILMGLLKKVLSRRRDIKLLVTSATMNSQKFSDFFGGAPQFTIPGRTYPVDIMFAKAPCSDYVEAAVRQVLQIHLSQPAGDILVFMTGQEDIEATCEIIADRLNQLHDAPRLSILPIYSQMPADLQAKIFDSAEPGVRKVVVATNIAETSLTVHGISYVVDTGYCKLKMYNSKLGIDTLQVTPISQANANQRAGRAGRTGPGIAYRLYTEMAYIREMFETTLPEIQRTNLSNTVLILKSLGVEEISDFDFMDRPPNDTLMASLYELWTLGALDNFGKLTTLGKKMSLFPMDPSLSKLIIIAEDYKCTEEIITIVSMLSVPSVFYRPKERAEESDAAREKFNVPESDHLMLLNIYQHWQRNGYSNSWCSKHFLHSKTLKRARDIRQQLVEIMSKQKISLESVSDWDIVRRVLCSAYFHQAACAKGIGEYVHLRSGMPCHLHVTSSLYGLGYLPDYVIYHELVLTSKEYMNIVTSVDPYWLAEFGGVYYSVKERFRNETESYDRVFSSKPQLDAQIAADRELDAKQKLAKNQEPVKSKRKSVIRAKPPRRVRGF

Target ORF information:

RefSeq Version	NM_001021796.3
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative ATP-dependent RNA helicase Prp16 (prp16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021796.3

ORF Insert Sequence:

ATGCTTTCAC ATAATCAAAA TGATTTGCTA TTTACGAAGT TAATAGATAA ATTAACTGAT 
TATATGTCCA GTAAAGATGC AGCTACATCG CTTGCCAGTC GAGTTTTGAC AATTGCCAAG 
GGTAGCTCGT CCAGCACCGA GTTTTCTAAT GCATTACATA CATTTGGACG ATTTTCCGAT 
AATGACAGCG TCCTTATTTA CGATTTATGC AAATCTATAA TTGGTTTAGA GACAAATTTG 
GGGATTTCTA AAGATAAACT TCCTGGGAAT GATGGGTCTC AGGTAGCTGG ATTAGTTTTG 
TCTAATCGTT CTGGTTTGAA AGGCCGAGAA CCCAAAAAAT CCCAGCTTGG ATTGGATGTT 
CTTGCTGTTC AAAAAAAGAA AGAGAAATCT CAAGTAGAAG GGCGCTCTGA ATTATCCCAA 
GTAGAAAATG ATTCCTTGGA GTCTATAGGA AACTATGACA GGGTGGAATT TAAGAGACCT 
AAAAATCCAC ATGAAAAACA TTTTCGTCCC CTTCGTCAAC GGTCATCTGT TGACGATCAT 
CAAGAGTTTG AAAGTGAAGA TGATAAATAT CGTAGAAATT CTTATTCTAG CTGGTCTGGA 
AGTAATTTCG AGTCTTCCAA TGGATCTAAT CGTCGGCGGT ATCGAACTCA AATGGAAGAA 
CCTTTATCCT CAAAACGCAG AAATCGGTTT GGTAATGGTT CTCGAAGAGA CGTCGATAGC 
TCTAAATATT CGCACGATAG TGATTATTCT TATGGTGCAC ACAGCTCTTG GGATGCCCGC 
GACGTTGAGT ACCCCGAAGA AGATCCCGAG AGTAAAGCTG ATCGTCAACG TTGGGAAGAG 
GAGCAAGCTC ACTTGGATCG TGATTGGTAC ATGAATTCCG AGTCGCAAAA TTTACTTGGC 
GATGAAGTTC ACAATCCATT CTCCGATTTT GAAACTGTCG AAGATCGTGC ACATGAAGCT 
GAGTTTATCG AAAAGCAGAA AAAGCATTTG TCAATTGAAG CATCAGACAG ATTTAAAGAA 
AATTCTATGT GGGAAAAAAA TAGAATGATC ACATCTGGTG TTTCAAAAGC TCCAGGGCTC 
GAATCTGATT ACTCCCTTAT GGAAGAGAGG CGTGTTCACT TGCTTGTCGA TGAACTGCGT 
CCTCATTTTT TGGATGGGGC CGAATTTTCT TCTAAGAAAG TCGGTGATAT TACATCGGTT 
CGAGATCCAC AAAGCGACCT TGCAATAAAT GCTAGATTAG GATCTCGTCT TGTACGTGAA 
CGAAGAGAAT TTCGGGAGCG TCAAAAAGCA GCAAGTGCTG CTACATCCCT TGCTGGTACA 
TCTTTGGGTA ACGTGATGGG TCTCAAAGAT TCTAATGATG AAGATGCCAA GGCCGGTACC 
ACACCTGTTA AAGTAGCTGG ACGTTCTGAA CAGTCGAATA AGAAGGATAC TGAGTTCGCT 
CGCACGAAAT CATATAGAGA ACAAAGAGAG TTCCTACCTG CCTTTGCCGT GCGTGAACAG 
CTTCTTTCTG TTATACGTGA TAACCAAGTT CTTATTGTAG TTGGTGAGAC TGGTTCTGGT 
AAAACTACCC AATTAGCTCA ATTCTTATAT GAGGATGGCT ATCATAGAAA TGGCATGATA 
GGGTGTACTC AACCTCGTCG TGTCGCCGCC ATGTCAGTTG CAAAAAGGGT TAGTGAGGAA 
ATGGGTGTTC GTCTAGGTTC AACAGTTGGT TATTCAATTC GTTTCGAAGA TGTTACTGGA 
CCGGATACCG TAATTAAGTA CATGACTGAC GGTGTTTTAC TAAGAGAATC CTTGATGCAA 
AATAATCTAG AAAAATATAG CGTCATTATA ATGGATGAAG CACATGAGCG ATCCCTTAAT 
ACAGATATTT TAATGGGTTT ACTAAAAAAG GTGTTATCCA GGAGAAGAGA TATCAAGTTG 
CTCGTTACAT CCGCTACTAT GAATTCCCAG AAATTCTCTG ATTTTTTTGG AGGCGCCCCA 
CAGTTTACAA TTCCAGGTCG TACTTACCCG GTGGATATTA TGTTTGCTAA AGCCCCTTGC 
TCTGATTACG TTGAAGCAGC TGTACGACAG GTATTGCAGA TTCACCTCTC TCAGCCTGCA 
GGTGATATCT TAGTTTTTAT GACAGGGCAG GAAGATATTG AAGCTACATG CGAGATCATT 
GCAGATAGGT TAAATCAGTT GCATGATGCA CCACGCTTGT CCATTTTGCC TATCTACTCA 
CAAATGCCTG CGGATTTACA AGCAAAAATT TTTGATTCCG CCGAGCCAGG TGTTAGAAAG 
GTTGTGGTCG CAACTAATAT TGCGGAAACT TCGCTCACTG TGCACGGAAT ATCATATGTT 
GTAGATACTG GATATTGTAA ACTGAAAATG TATAATTCTA AGTTGGGTAT TGATACTCTG 
CAAGTTACAC CTATTTCGCA AGCTAATGCG AATCAAAGAG CCGGTCGTGC TGGGCGTACT 
GGACCCGGTA TTGCATATCG CCTTTATACC GAAATGGCTT ATATTCGTGA AATGTTTGAA 
ACGACCTTAC CTGAAATTCA GCGTACCAAT CTATCAAACA CAGTTCTTAT TTTAAAATCC 
CTTGGTGTTG AAGAAATTTC GGATTTTGAT TTCATGGACC GTCCTCCAAA TGATACGTTA 
ATGGCATCTC TGTATGAATT ATGGACTTTA GGAGCCCTCG ACAATTTTGG AAAACTTACT 
ACTTTAGGAA AGAAAATGTC GTTATTTCCA ATGGATCCTT CTCTTTCAAA ACTTATTATT 
ATTGCTGAAG ATTATAAATG TACTGAGGAA ATTATTACGA TTGTTTCCAT GCTTTCAGTT 
CCGTCTGTAT TTTATAGACC CAAGGAAAGA GCTGAAGAGA GTGACGCTGC AAGAGAAAAG 
TTTAATGTGC CAGAAAGTGA TCATTTAATG CTACTCAATA TTTATCAGCA TTGGCAAAGA 
AATGGATATT CAAATTCATG GTGCAGTAAA CATTTTTTAC ACTCGAAAAC TCTTAAACGG 
GCGCGCGATA TTCGTCAACA GCTGGTCGAA ATTATGTCAA AGCAAAAGAT TAGTTTAGAA 
TCTGTATCCG ATTGGGACAT TGTTCGGAGA GTTTTGTGTT CTGCGTACTT TCATCAAGCA 
GCATGCGCTA AAGGTATTGG GGAGTATGTT CATTTACGAA GCGGCATGCC TTGCCATCTA 
CATGTTACTT CGAGTCTTTA TGGCTTAGGG TATCTACCTG ATTATGTTAT ATATCACGAA 
CTTGTACTCA CTTCCAAGGA GTACATGAAT ATTGTCACCA GTGTTGATCC ATACTGGCTA 
GCTGAGTTTG GTGGAGTTTA CTACTCTGTG AAGGAAAGAT TCCGAAATGA AACTGAAAGC 
TATGATCGAG TTTTTTCTAG CAAACCTCAA TTGGATGCAC AGATAGCCGC CGACCGAGAA 
CTTGATGCAA AGCAAAAGCT TGCGAAGAAT CAAGAACCCG TGAAATCAAA ACGAAAGTCA 
GTGATACGTG CCAAACCTCC TAGAAGGGTT CGTGGATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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prp16 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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