Dhx38 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dhx38
Entrez Gene ID	292007
Full Name	DEAH-box helicase 38
General protein information	Preferred Names DEAH-box helicase 38 Names pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 DEAH (Asp-Glu-Ala-His) box polypeptide 38
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106185.1	NP_001099655.1	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
XM_006255577.2	XP_006255639.1	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 isoform X1
XM_008772489.2	XP_008770711.1	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DHX38	XP_511101.3
Bos taurus (cattle)	DHX38	NP_001069266.1
Mus musculus (house mouse)	Dhx38	NP_848467.1
Danio rerio (zebrafish)	dhx38	NP_957170.1
Saccharomyces cerevisiae (baker's yeast)	PRP16	NP_013012.3
Kluyveromyces lactis	KLLA0A07733g	XP_451344.1
Eremothecium gossypii	AGOS_ADR224W	NP_984320.1
Oryza sativa (rice)	Os07g0508000	NP_001059742.1
Xenopus tropicalis (tropical clawed frog)	dhx38	XP_002937626.2
	DHX38	XP_001097681.2
Canis lupus familiaris (dog)	DHX38	XP_005620809.1
Gallus gallus (chicken)	DHX38	NP_001025753.1
Caenorhabditis elegans (roundworm)	mog-1	NP_499212.1
Schizosaccharomyces pombe (fission yeast)	prp16	NP_595890.2
Rattus norvegicus (Norway rat)	Dhx38	NP_001099655.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008528	XP_316912.4
Homo sapiens (human)	DHX38	NP_054722.2
Drosophila melanogaster (fruit fly)	l(1)G0007	NP_572947.1
Magnaporthe oryzae (rice blast fungus)	MGG_11351	XP_003713740.1
Neurospora crassa	NCU02728	XP_965113.1
Arabidopsis thaliana (thale cress)	EMB3011	NP_196805.2

Gene Ontology
Bibliography

Related articles in PubMed

Functions of the DExD/H-box proteins in nuclear pre-mRNA splicing.
Chang TH, Tung L, Yeh FL, Chen JH, Chang SL
Biochimica et biophysica acta1829(8)764-74(2013 Aug)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.
Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ
RNA (New York, N.Y.)8(4)426-39(2002 Apr)

Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing.
Zhou Z, Reed R
The EMBO journal17(7)2095-106(1998 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dhx38 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dhx38 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04503	NM_001106185.1 Latest version!	Rattus norvegicus DEAH-box helicase 38 (Dhx38), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa38917	XM_006255577.2 Latest version!	Rattus norvegicus DEAH-box helicase 38 (Dhx38), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa38917	XM_008772489.2 Latest version!	Rattus norvegicus DEAH-box helicase 38 (Dhx38), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dhx38 ( NM_001106185.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099655.1 Rattus norvegicus DEAH-box helicase 38 (Dhx38), mRNA.

Price & Availability↑

CloneID	ORa04503
Clone ID Related Accession (Same CDS sequence)	NM_001106185.1
Accession Version	NM_001106185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473972.1. On or before Oct 4, 2007 this sequence version replaced XM_238048.4, XM_001075164.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGACG CTGGTGAGGA CGCCTCCATC CATCGCTTGG AAGGCACCAA CTTGGACTCT 
CAGGTTGGTG GCCTTATTTG CAAGACCAAA AGTGCAGCCA GTGAGCAGCA TGTCTTCAAA 
GCCCCTGCTC CTCGCCCTTC ATTGCTGGGA CTGGACTTAC TGGCGTCCCT GAAACGGAGG 
GAGCGAGAGG AGAAGGATGA TGGGGAGGAC AAGAAAAAGT CCAGAATCTC TTCCTACAAA 
GACTGGGAGG AGAGTAAGGA TGACCAGAAG GATGCCGAGG AGGATGGCGA TGAGCAGGCT 
GGCCGAAGTA GCCGGAGAGA CAGTGAAGAG TTTTGGGAAC GCAGTAGGCA GAGAGAGCGG 
GATCGGAGGG AGCATGGAGT CTACGCCTCA TCCAAAGAAG ACAAGGATCG GAAGAAGGAG 
AAATCCCGGG ATCGAGACCA TGACCGCAAG AGAGACAGAG ATGAGCGGGA CAGAAGTAGG 
CACAGCAGCA GATCAGAACG AGACGGAGGC TCAGAGCGCA GCAGCAGAAG AAATGAACCA 
GAGAGTCCTC GACACCGCCC AAAAGATGCA GCCACCCCTT CAAGGTCCAC CTGGGAGGAG 
GAGGACAGTG GCTATGGCTC TTCAAGGCGC TCTCAGTGGG AAACACCCTC CCCAACACCT 
TCCTATCGGG ACTCTGAGCG GAGTCATCGG CCGTCCACAC GAGATCGAGA CAGGTCTGTG 
AGGAGCAAGT CCTCAGATGA CACACCACTG CCGACCCCCT CCTATAAGTA CAACGAGTGG 
GCTGATGATA GAAGACACTT GGGGTCTACC CCACGTCTGT CCAGGGGCCG AGGAAGACGT 
GAGGATGGTG AAGAAGGGAT TGCATTTGAC ACGGAGGAGG AGCGGCAGCA GTGGGAGGAC 
GACCAGAGGC AAGCAGACCG GGATTGGTAC ATGATGGACG AGGGCTATGA TGAGTTCCAC 
AACCCTCTGG CTTATTCCTC TGAGGACTAC GTGAGGCGGC GGGAGCAGCA CCTGCACAAA 
CAGAAGCAGA AGCGCATTTC AGCACAGCGC AGGCAGATCA ATGAGGATAA TGAGCGTTGG 
GAGACTAACC GGATGCTCAC CAGCGGGGTG GTCCATCGGC TGGAGGTGGA TGAGGACTTT 
GAAGAGGACA ACGCGGCCAA GGTGCATCTG ATGGTGCACA ACCTGGTGCC TCCCTTTCTG 
GATGGACGGA TTGTTTTCAC CAAGCAGCCG GAGCCTGTGA TCCCAGTGAA GGATGCCACC 
TCAGACCTGG CCATTATTGC TCGGAAAGGC AGCCAGACGG TCCGCAAGCA CAGGGAACAG 
AAAGAACGGA GAAAGGCTCA GCACAAACAC TGGGAACTGG CAGGGACCAA ACTAGGAGAC 
ATAATGGGTG TCAAAAAAGA AGAGGAGCCA GATAAAGCTA TGACTGAAGA TGGGAAAGTG 
GACTACAGGA CAGAGCAGAA GTTTGCAGAT CACATGAAGG AGAAAAGTGA GGCCAGCAGT 
GAGTTTGCGA AGAAGAAGTC GATCCTGGAG CAGAGGCAAT ACCTGCCCAT CTTTGCTGTA 
CAGCAGGAAC TCCTCACCAT CATCAGAGAC AACAGCATTG TGATCGTGGT CGGGGAGACG 
GGGAGTGGCA AGACCACTCA GCTGACCCAG TACTTGCATC AAGATGGGTA CACGGACTAT 
GGGATGATCG GGTGCACACA GCCCCGGCGT GTGGCTGCCA TGTCAGTGGC CAAGAGAGTC 
AGTGAAGAGA TGGGGGGAAA CCTTGGAGAA GAGGTGGGTT ATGCCATTCG CTTTGAAGAC 
TGCACTTCTG AAAACACCTT GATCAAATAC ATGACCGATG GGATCCTGCT CCGAGAGTCC 
CTGCGGGAGG CTGACCTAGA CCACTACAGC GCCATCATCA TGGATGAGGC CCATGAGCGC 
TCCCTCAACA CCGACGTGCT CTTTGGGCTG CTCCGGGAGG TTGTGGCTCG ACGCTCAGAC 
CTGAAGCTCA TTGTCACCTC AGCTACTATG GATGCAGACA AATTTGCTGC CTTCTTTGGG 
AATGTACCCA TCTTCCACAT CCCTGGGCGA ACTTTTCCTG TTGACATCCT CTTCAGCAAG 
ACCCCACAGG AGGATTATGT GGAGGCCGCA GTGAAGCAGT CCTTGCAGGT GCACCTGTCT 
GGGGCCCCTG GAGACATCCT TATCTTCATG CCTGGCCAAG AGGACATTGA GGTGACGTCT 
GACCAGATCG TAGAGCACCT AGAGGAGCTG GAGAACGCGC CTGCCTTGGC TGTGCTGCCC 
ATCTACTCCC AGCTGCCGTC TGACCTTCAG GCAAAAATCT TCCAGAAGGC TCCCGATGGC 
GTCCGGAAGT GCATCGTTGC TACCAACATT GCTGAGACGT CTCTGACTGT TGACGGCATC 
ATGTTTGTCA TCGACTCTGG CTACTGCAAG TTAAAGGTCT TCAACCCCAG GATCGGCATG 
GATGCTCTGC AGATCTACCC CATTAGCCAG GCCAATGCCA ACCAGAGGTC AGGGAGAGCT 
GGCAGGACGG GCCCAGGTCA GTGTTTCAGG CTCTACACAC AGAGCGCTTA CAAGAATGAG 
CTCCTGACTA CCACTGTGCC TGAGATCCAG AGGACCAACC TTGCCAACGT GGTGCTGCTG 
CTCAAGTCCC TGGGGGTGCA GGACCTGCTG CAGTTCCACT TCATGGATCC ACCCCCAGAG 
GACAACATGC TCAACTCCAT GTACCAGCTG TGGATCCTCG GAGCCCTGGA TAACACAGGT 
GGTCTCACTT CCACGGGGCG GCTGATGGTG GAGTTCCCAC TGGACCCGGC TCTCTCCAAG 
ATGCTCATCG TGTCTTGTGA CATGGGCTGC AGCTCCGAGA TCCTGCTCAT CGTCTCCATG 
CTCTCCGTCC CTGCCATCTT CTACAGGCCC AAGGGCAGAG AGGAGGAGAG CGATCAGATC 
CGGGAGAAGT TTGCTGTCCC TGAGAGTGAT CACCTGACCT ACCTGAACGT CTACCTGCAG 
TGGAAGAACA ACAATTACTC CACCATTTGG TGCAACGACC ACTTCATCCA CGCCAAGGCC 
ATGCGGAAGG TCCGGGAGGT GCGGGCCCAG CTCAAGGATA TCATGGTGCA GCAGCGGATG 
AGCCTGGCCT CATGTGGCAC TGACTGGGAC ATTGTCAGGA AGTGCATCTG TGCTGCCTAT 
TTCCACCAGG CAGCCAAGCT CAAGGGCATT GGGGAGTATG TGAACATCCG TACAGGGATG 
CCCTGCCACT TACACCCCAC CAGCTCCCTC TTCGGAATGG GTTACACCCC TGACTACATT 
GTATATCACG AGCTGGTCAT GACCACCAAG GAGTACATGC AGTGTGTGAC CGCCGTGGAT 
GGAGAGTGGC TGGCGGAGCT GGGCCCCATG TTCTACAGTG TGAAACAGGC AGGCAAGTCT 
CGGCAGGAGA ATCGTCGTCG GGCTAAAGAG GAGGCCTCTG CCATGGAAGA GGAAATGGCC 
CTAGCTGAGG AGCAGCTGCG CGCCCGGCGG CAGGAGCAGG AGAAGCGCAG CCCGCTGGGC 
AGTGTCAGGT CTACAAAGAT CTACACTCCA GGACGGAAAG AACAGGGGGA ACCCATGACA 
CCTCGCCGCA CACCAGCCCG CTTTGGACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099655.1
CDS	389..4021
Translation	MEDAGEDASIHRLEGTNLDSQVGGLICKTKSAASEQHVFKAPAPRPSLLGLDLLASLKRREREEKDDGEDKKKSRISSYKDWEESKDDQKDAEEDGDEQAGRSSRRDSEEFWERSRQRERDRREHGVYASSKEDKDRKKEKSRDRDHDRKRDRDERDRSRHSSRSERDGGSERSSRRNEPESPRHRPKDAATPSRSTWEEEDSGYGSSRRSQWETPSPTPSYRDSERSHRPSTRDRDRSVRSKSSDDTPLPTPSYKYNEWADDRRHLGSTPRLSRGRGRREDGEEGIAFDTEEERQQWEDDQRQADRDWYMMDEGYDEFHNPLAYSSEDYVRRREQHLHKQKQKRISAQRRQINEDNERWETNRMLTSGVVHRLEVDEDFEEDNAAKVHLMVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDLAIIARKGSQTVRKHREQKERRKAQHKHWELAGTKLGDIMGVKKEEEPDKAMTEDGKVDYRTEQKFADHMKEKSEASSEFAKKKSILEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHQDGYTDYGMIGCTQPRRVAAMSVAKRVSEEMGGNLGEEVGYAIRFEDCTSENTLIKYMTDGILLRESLREADLDHYSAIIMDEAHERSLNTDVLFGLLREVVARRSDLKLIVTSATMDADKFAAFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQSLQVHLSGAPGDILIFMPGQEDIEVTSDQIVEHLEELENAPALAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGIMFVIDSGYCKLKVFNPRIGMDALQIYPISQANANQRSGRAGRTGPGQCFRLYTQSAYKNELLTTTVPEIQRTNLANVVLLLKSLGVQDLLQFHFMDPPPEDNMLNSMYQLWILGALDNTGGLTSTGRLMVEFPLDPALSKMLIVSCDMGCSSEILLIVSMLSVPAIFYRPKGREEESDQIREKFAVPESDHLTYLNVYLQWKNNNYSTIWCNDHFIHAKAMRKVREVRAQLKDIMVQQRMSLASCGTDWDIVRKCICAAYFHQAAKLKGIGEYVNIRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSVKQAGKSRQENRRRAKEEASAMEEEMALAEEQLRARRQEQEKRSPLGSVRSTKIYTPGRKEQGEPMTPRRTPARFGL

Target ORF information:

RefSeq Version	NM_001106185.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAH-box helicase 38 (Dhx38), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106185.1

ORF Insert Sequence:

ATGGAGGACG CTGGTGAGGA CGCCTCCATC CATCGCTTGG AAGGCACCAA CTTGGACTCT 
CAGGTTGGTG GCCTTATTTG CAAGACCAAA AGTGCAGCCA GTGAGCAGCA TGTCTTCAAA 
GCCCCTGCTC CTCGCCCTTC ATTGCTGGGA CTGGACTTAC TGGCGTCCCT GAAACGGAGG 
GAGCGAGAGG AGAAGGATGA TGGGGAGGAC AAGAAAAAGT CCAGAATCTC TTCCTACAAA 
GACTGGGAGG AGAGTAAGGA TGACCAGAAG GATGCCGAGG AGGATGGCGA TGAGCAGGCT 
GGCCGAAGTA GCCGGAGAGA CAGTGAAGAG TTTTGGGAAC GCAGTAGGCA GAGAGAGCGG 
GATCGGAGGG AGCATGGAGT CTACGCCTCA TCCAAAGAAG ACAAGGATCG GAAGAAGGAG 
AAATCCCGGG ATCGAGACCA TGACCGCAAG AGAGACAGAG ATGAGCGGGA CAGAAGTAGG 
CACAGCAGCA GATCAGAACG AGACGGAGGC TCAGAGCGCA GCAGCAGAAG AAATGAACCA 
GAGAGTCCTC GACACCGCCC AAAAGATGCA GCCACCCCTT CAAGGTCCAC CTGGGAGGAG 
GAGGACAGTG GCTATGGCTC TTCAAGGCGC TCTCAGTGGG AAACACCCTC CCCAACACCT 
TCCTATCGGG ACTCTGAGCG GAGTCATCGG CCGTCCACAC GAGATCGAGA CAGGTCTGTG 
AGGAGCAAGT CCTCAGATGA CACACCACTG CCGACCCCCT CCTATAAGTA CAACGAGTGG 
GCTGATGATA GAAGACACTT GGGGTCTACC CCACGTCTGT CCAGGGGCCG AGGAAGACGT 
GAGGATGGTG AAGAAGGGAT TGCATTTGAC ACGGAGGAGG AGCGGCAGCA GTGGGAGGAC 
GACCAGAGGC AAGCAGACCG GGATTGGTAC ATGATGGACG AGGGCTATGA TGAGTTCCAC 
AACCCTCTGG CTTATTCCTC TGAGGACTAC GTGAGGCGGC GGGAGCAGCA CCTGCACAAA 
CAGAAGCAGA AGCGCATTTC AGCACAGCGC AGGCAGATCA ATGAGGATAA TGAGCGTTGG 
GAGACTAACC GGATGCTCAC CAGCGGGGTG GTCCATCGGC TGGAGGTGGA TGAGGACTTT 
GAAGAGGACA ACGCGGCCAA GGTGCATCTG ATGGTGCACA ACCTGGTGCC TCCCTTTCTG 
GATGGACGGA TTGTTTTCAC CAAGCAGCCG GAGCCTGTGA TCCCAGTGAA GGATGCCACC 
TCAGACCTGG CCATTATTGC TCGGAAAGGC AGCCAGACGG TCCGCAAGCA CAGGGAACAG 
AAAGAACGGA GAAAGGCTCA GCACAAACAC TGGGAACTGG CAGGGACCAA ACTAGGAGAC 
ATAATGGGTG TCAAAAAAGA AGAGGAGCCA GATAAAGCTA TGACTGAAGA TGGGAAAGTG 
GACTACAGGA CAGAGCAGAA GTTTGCAGAT CACATGAAGG AGAAAAGTGA GGCCAGCAGT 
GAGTTTGCGA AGAAGAAGTC GATCCTGGAG CAGAGGCAAT ACCTGCCCAT CTTTGCTGTA 
CAGCAGGAAC TCCTCACCAT CATCAGAGAC AACAGCATTG TGATCGTGGT CGGGGAGACG 
GGGAGTGGCA AGACCACTCA GCTGACCCAG TACTTGCATC AAGATGGGTA CACGGACTAT 
GGGATGATCG GGTGCACACA GCCCCGGCGT GTGGCTGCCA TGTCAGTGGC CAAGAGAGTC 
AGTGAAGAGA TGGGGGGAAA CCTTGGAGAA GAGGTGGGTT ATGCCATTCG CTTTGAAGAC 
TGCACTTCTG AAAACACCTT GATCAAATAC ATGACCGATG GGATCCTGCT CCGAGAGTCC 
CTGCGGGAGG CTGACCTAGA CCACTACAGC GCCATCATCA TGGATGAGGC CCATGAGCGC 
TCCCTCAACA CCGACGTGCT CTTTGGGCTG CTCCGGGAGG TTGTGGCTCG ACGCTCAGAC 
CTGAAGCTCA TTGTCACCTC AGCTACTATG GATGCAGACA AATTTGCTGC CTTCTTTGGG 
AATGTACCCA TCTTCCACAT CCCTGGGCGA ACTTTTCCTG TTGACATCCT CTTCAGCAAG 
ACCCCACAGG AGGATTATGT GGAGGCCGCA GTGAAGCAGT CCTTGCAGGT GCACCTGTCT 
GGGGCCCCTG GAGACATCCT TATCTTCATG CCTGGCCAAG AGGACATTGA GGTGACGTCT 
GACCAGATCG TAGAGCACCT AGAGGAGCTG GAGAACGCGC CTGCCTTGGC TGTGCTGCCC 
ATCTACTCCC AGCTGCCGTC TGACCTTCAG GCAAAAATCT TCCAGAAGGC TCCCGATGGC 
GTCCGGAAGT GCATCGTTGC TACCAACATT GCTGAGACGT CTCTGACTGT TGACGGCATC 
ATGTTTGTCA TCGACTCTGG CTACTGCAAG TTAAAGGTCT TCAACCCCAG GATCGGCATG 
GATGCTCTGC AGATCTACCC CATTAGCCAG GCCAATGCCA ACCAGAGGTC AGGGAGAGCT 
GGCAGGACGG GCCCAGGTCA GTGTTTCAGG CTCTACACAC AGAGCGCTTA CAAGAATGAG 
CTCCTGACTA CCACTGTGCC TGAGATCCAG AGGACCAACC TTGCCAACGT GGTGCTGCTG 
CTCAAGTCCC TGGGGGTGCA GGACCTGCTG CAGTTCCACT TCATGGATCC ACCCCCAGAG 
GACAACATGC TCAACTCCAT GTACCAGCTG TGGATCCTCG GAGCCCTGGA TAACACAGGT 
GGTCTCACTT CCACGGGGCG GCTGATGGTG GAGTTCCCAC TGGACCCGGC TCTCTCCAAG 
ATGCTCATCG TGTCTTGTGA CATGGGCTGC AGCTCCGAGA TCCTGCTCAT CGTCTCCATG 
CTCTCCGTCC CTGCCATCTT CTACAGGCCC AAGGGCAGAG AGGAGGAGAG CGATCAGATC 
CGGGAGAAGT TTGCTGTCCC TGAGAGTGAT CACCTGACCT ACCTGAACGT CTACCTGCAG 
TGGAAGAACA ACAATTACTC CACCATTTGG TGCAACGACC ACTTCATCCA CGCCAAGGCC 
ATGCGGAAGG TCCGGGAGGT GCGGGCCCAG CTCAAGGATA TCATGGTGCA GCAGCGGATG 
AGCCTGGCCT CATGTGGCAC TGACTGGGAC ATTGTCAGGA AGTGCATCTG TGCTGCCTAT 
TTCCACCAGG CAGCCAAGCT CAAGGGCATT GGGGAGTATG TGAACATCCG TACAGGGATG 
CCCTGCCACT TACACCCCAC CAGCTCCCTC TTCGGAATGG GTTACACCCC TGACTACATT 
GTATATCACG AGCTGGTCAT GACCACCAAG GAGTACATGC AGTGTGTGAC CGCCGTGGAT 
GGAGAGTGGC TGGCGGAGCT GGGCCCCATG TTCTACAGTG TGAAACAGGC AGGCAAGTCT 
CGGCAGGAGA ATCGTCGTCG GGCTAAAGAG GAGGCCTCTG CCATGGAAGA GGAAATGGCC 
CTAGCTGAGG AGCAGCTGCG CGCCCGGCGG CAGGAGCAGG AGAAGCGCAG CCCGCTGGGC 
AGTGTCAGGT CTACAAAGAT CTACACTCCA GGACGGAAAG AACAGGGGGA ACCCATGACA 
CCTCGCCGCA CACCAGCCCG CTTTGGACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx38 ( XM_006255577.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255639.1 Rattus norvegicus DEAH-box helicase 38 (Dhx38), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38917
Clone ID Related Accession (Same CDS sequence)	XM_006255577.2 , XM_008772489.2
Accession Version	XM_006255577.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006255577.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG CTGGTGAGGA CGCCTCCATC CATCGCTTGG AAGGCACCAA CTTGGACTCT 
CAGGTTGGTG GCCTTATTTG CAAGACCAAA AGTGCAGCCA GTGAGCAGCA TGTCTTCAAA 
GCCCCTGCTC CTCGCCCTTC ATTGCTGGGA CTGGACTTAC TGGCGTCCCT GAAACGGAGG 
GAGCGAGAGG AGAAGGATGA TGGGGAGGAC AAGAAAAAGT CCAGAATCTC TTCCTACAAA 
GACTGGGAGG AGAGTAAGGA TGACCAGAAG GATGCCGAGG AGGATGGCGA TGAGCAGGCT 
GGCCGAAGTA GCCGGAGAGA CAGGCACTAC CGATCTGCTC GGGTAGAGAC TCCATCCCAC 
CCCGGGGGTG TGAGTGAAGA GTTTTGGGAA CGCAGTAGGC AGAGAGAGCG GGATCGGAGG 
GAGCATGGAG TCTACGCCTC ATCCAAAGAA GACAAGGATC GGAAGAAGGA GAAATCCCGG 
GATCGAGACC ATGACCGCAA GAGAGACAGA GATGAGCGGG ACAGAAGTAG GCACAGCAGC 
AGATCAGAAC GAGACGGAGG CTCAGAGCGC AGCAGCAGAA GAAATGAACC AGAGAGTCCT 
CGACACCGCC CAAAAGATGC AGCCACCCCT TCAAGGTCCA CCTGGGAGGA GGAGGACAGT 
GGCTATGGCT CTTCAAGGCG CTCTCAGTGG GAAACACCCT CCCCAACACC TTCCTATCGG 
GACTCTGAGC GGAGTCATCG GCCGTCCACA CGAGATCGAG ACAGGTCTGT GAGGAGCAAG 
TCCTCAGATG ACACACCACT GCCGACCCCC TCCTATAAGT ACAACGAGTG GGCTGATGAT 
AGAAGACACT TGGGGTCTAC CCCACGTCTG TCCAGGGGCC GAGGAAGACG TGAGGATGGT 
GAAGAAGGGA TTGCATTTGA CACGGAGGAG GAGCGGCAGC AGTGGGAGGA CGACCAGAGG 
CAAGCAGACC GGGATTGGTA CATGATGGAC GAGGGCTATG ATGAGTTCCA CAACCCTCTG 
GCTTATTCCT CTGAGGACTA CGTGAGGCGG CGGGAGCAGC ACCTGCACAA ACAGAAGCAG 
AAGCGCATTT CAGCACAGCG CAGGCAGATC AATGAGGATA ATGAGCGTTG GGAGACTAAC 
CGGATGCTCA CCAGCGGGGT GGTCCATCGG CTGGAGGTGG ATGAGGACTT TGAAGAGGAC 
AACGCGGCCA AGGTGCATCT GATGGTGCAC AACCTGGTGC CTCCCTTTCT GGATGGACGG 
ATTGTTTTCA CCAAGCAGCC GGAGCCTGTG ATCCCAGTGA AGGATGCCAC CTCAGACCTG 
GCCATTATTG CTCGGAAAGG CAGCCAGACG GTCCGCAAGC ACAGGGAACA GAAAGAACGG 
AGAAAGGCTC AGCACAAACA CTGGGAACTG GCAGGGACCA AACTAGGAGA CATAATGGGT 
GTCAAAAAAG AAGAGGAGCC AGATAAAGCT ATGACTGAAG ATGGGAAAGT GGACTACAGG 
ACAGAGCAGA AGTTTGCAGA TCACATGAAG GAGAAAAGTG AGGCCAGCAG TGAGTTTGCG 
AAGAAGAAGT CGATCCTGGA GCAGAGGCAA TACCTGCCCA TCTTTGCTGT ACAGCAGGAA 
CTCCTCACCA TCATCAGAGA CAACAGCATT GTGATCGTGG TCGGGGAGAC GGGGAGTGGC 
AAGACCACTC AGCTGACCCA GTACTTGCAT CAAGATGGGT ACACGGACTA TGGGATGATC 
GGGTGCACAC AGCCCCGGCG TGTGGCTGCC ATGTCAGTGG CCAAGAGAGT CAGTGAAGAG 
ATGGGGGGAA ACCTTGGAGA AGAGGTGGGT TATGCCATTC GCTTTGAAGA CTGCACTTCT 
GAAAACACCT TGATCAAATA CATGACCGAT GGGATCCTGC TCCGAGAGTC CCTGCGGGAG 
GCTGACCTAG ACCACTACAG CGCCATCATC ATGGATGAGG CCCATGAGCG CTCCCTCAAC 
ACCGACGTGC TCTTTGGGCT GCTCCGGGAG GTTGTGGCTC GACGCTCAGA CCTGAAGCTC 
ATTGTCACCT CAGCTACTAT GGATGCAGAC AAATTTGCTG CCTTCTTTGG GAATGTACCC 
ATCTTCCACA TCCCTGGGCG AACTTTTCCT GTTGACATCC TCTTCAGCAA GACCCCACAG 
GAGGATTATG TGGAGGCCGC AGTGAAGCAG TCCTTGCAGG TGCACCTGTC TGGGGCCCCT 
GGAGACATCC TTATCTTCAT GCCTGGCCAA GAGGACATTG AGGTGACGTC TGACCAGATC 
GTAGAGCACC TAGAGGAGCT GGAGAACGCG CCTGCCTTGG CTGTGCTGCC CATCTACTCC 
CAGCTGCCGT CTGACCTTCA GGCAAAAATC TTCCAGAAGG CTCCCGATGG CGTCCGGAAG 
TGCATCGTTG CTACCAACAT TGCTGAGACG TCTCTGACTG TTGACGGCAT CATGTTTGTC 
ATCGACTCTG GCTACTGCAA GTTAAAGGTC TTCAACCCCA GGATCGGCAT GGATGCTCTG 
CAGATCTACC CCATTAGCCA GGCCAATGCC AACCAGAGGT CAGGGAGAGC TGGCAGGACG 
GGCCCAGGTC AGTGTTTCAG GCTCTACACA CAGAGCGCTT ACAAGAATGA GCTCCTGACT 
ACCACTGTGC CTGAGATCCA GAGGACCAAC CTTGCCAACG TGGTGCTGCT GCTCAAGTCC 
CTGGGGGTGC AGGACCTGCT GCAGTTCCAC TTCATGGATC CACCCCCAGA GGACAACATG 
CTCAACTCCA TGTACCAGCT GTGGATCCTC GGAGCCCTGG ATAACACAGG TGGTCTCACT 
TCCACGGGGC GGCTGATGGT GGAGTTCCCA CTGGACCCGG CTCTCTCCAA GATGCTCATC 
GTGTCTTGTG ACATGGGCTG CAGCTCCGAG ATCCTGCTCA TCGTCTCCAT GCTCTCCGTC 
CCTGCCATCT TCTACAGGCC CAAGGGCAGA GAGGAGGAGA GCGATCAGAT CCGGGAGAAG 
TTTGCTGTCC CTGAGAGTGA TCACCTGACC TACCTGAACG TCTACCTGCA GTGGAAGAAC 
AACAATTACT CCACCATTTG GTGCAACGAC CACTTCATCC ACGCCAAGGC CATGCGGAAG 
GTCCGGGAGG TGCGGGCCCA GCTCAAGGAT ATCATGGTGC AGCAGCGGAT GAGCCTGGCC 
TCATGTGGCA CTGACTGGGA CATTGTCAGG AAGTGCATCT GTGCTGCCTA TTTCCACCAG 
GCAGCCAAGC TCAAGGGCAT TGGGGAGTAT GTGAACATCC GTACAGGGAT GCCCTGCCAC 
TTACACCCCA CCAGCTCCCT CTTCGGAATG GGTTACACCC CTGACTACAT TGTATATCAC 
GAGCTGGTCA TGACCACCAA GGAGTACATG CAGTGTGTGA CCGCCGTGGA TGGAGAGTGG 
CTGGCGGAGC TGGGCCCCAT GTTCTACAGT GTGAAACAGG CAGGCAAGTC TCGGCAGGAG 
AATCGTCGTC GGGCTAAAGA GGAGGCCTCT GCCATGGAAG AGGAAATGGC CCTAGCTGAG 
GAGCAGCTGC GCGCCCGGCG GCAGGAGCAG GAGAAGCGCA GCCCGCTGGG CAGTGTCAGG 
TCTACAAAGA TCTACACTCC AGGACGGAAA GAACAGGGGG AACCCATGAC ACCTCGCCGC 
ACACCAGCCC GCTTTGGACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255639.1
CDS	384..4067
Translation	MEDAGEDASIHRLEGTNLDSQVGGLICKTKSAASEQHVFKAPAPRPSLLGLDLLASLKRREREEKDDGEDKKKSRISSYKDWEESKDDQKDAEEDGDEQAGRSSRRDRHYRSARVETPSHPGGVSEEFWERSRQRERDRREHGVYASSKEDKDRKKEKSRDRDHDRKRDRDERDRSRHSSRSERDGGSERSSRRNEPESPRHRPKDAATPSRSTWEEEDSGYGSSRRSQWETPSPTPSYRDSERSHRPSTRDRDRSVRSKSSDDTPLPTPSYKYNEWADDRRHLGSTPRLSRGRGRREDGEEGIAFDTEEERQQWEDDQRQADRDWYMMDEGYDEFHNPLAYSSEDYVRRREQHLHKQKQKRISAQRRQINEDNERWETNRMLTSGVVHRLEVDEDFEEDNAAKVHLMVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDLAIIARKGSQTVRKHREQKERRKAQHKHWELAGTKLGDIMGVKKEEEPDKAMTEDGKVDYRTEQKFADHMKEKSEASSEFAKKKSILEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHQDGYTDYGMIGCTQPRRVAAMSVAKRVSEEMGGNLGEEVGYAIRFEDCTSENTLIKYMTDGILLRESLREADLDHYSAIIMDEAHERSLNTDVLFGLLREVVARRSDLKLIVTSATMDADKFAAFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQSLQVHLSGAPGDILIFMPGQEDIEVTSDQIVEHLEELENAPALAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGIMFVIDSGYCKLKVFNPRIGMDALQIYPISQANANQRSGRAGRTGPGQCFRLYTQSAYKNELLTTTVPEIQRTNLANVVLLLKSLGVQDLLQFHFMDPPPEDNMLNSMYQLWILGALDNTGGLTSTGRLMVEFPLDPALSKMLIVSCDMGCSSEILLIVSMLSVPAIFYRPKGREEESDQIREKFAVPESDHLTYLNVYLQWKNNNYSTIWCNDHFIHAKAMRKVREVRAQLKDIMVQQRMSLASCGTDWDIVRKCICAAYFHQAAKLKGIGEYVNIRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSVKQAGKSRQENRRRAKEEASAMEEEMALAEEQLRARRQEQEKRSPLGSVRSTKIYTPGRKEQGEPMTPRRTPARFGL

Target ORF information:

RefSeq Version	XM_006255577.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAH-box helicase 38 (Dhx38), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255577.2

ORF Insert Sequence:

ATGGAGGACG CTGGTGAGGA CGCCTCCATC CATCGCTTGG AAGGCACCAA CTTGGACTCT 
CAGGTTGGTG GCCTTATTTG CAAGACCAAA AGTGCAGCCA GTGAGCAGCA TGTCTTCAAA 
GCCCCTGCTC CTCGCCCTTC ATTGCTGGGA CTGGACTTAC TGGCGTCCCT GAAACGGAGG 
GAGCGAGAGG AGAAGGATGA TGGGGAGGAC AAGAAAAAGT CCAGAATCTC TTCCTACAAA 
GACTGGGAGG AGAGTAAGGA TGACCAGAAG GATGCCGAGG AGGATGGCGA TGAGCAGGCT 
GGCCGAAGTA GCCGGAGAGA CAGGCACTAC CGATCTGCTC GGGTAGAGAC TCCATCCCAC 
CCCGGGGGTG TGAGTGAAGA GTTTTGGGAA CGCAGTAGGC AGAGAGAGCG GGATCGGAGG 
GAGCATGGAG TCTACGCCTC ATCCAAAGAA GACAAGGATC GGAAGAAGGA GAAATCCCGG 
GATCGAGACC ATGACCGCAA GAGAGACAGA GATGAGCGGG ACAGAAGTAG GCACAGCAGC 
AGATCAGAAC GAGACGGAGG CTCAGAGCGC AGCAGCAGAA GAAATGAACC AGAGAGTCCT 
CGACACCGCC CAAAAGATGC AGCCACCCCT TCAAGGTCCA CCTGGGAGGA GGAGGACAGT 
GGCTATGGCT CTTCAAGGCG CTCTCAGTGG GAAACACCCT CCCCAACACC TTCCTATCGG 
GACTCTGAGC GGAGTCATCG GCCGTCCACA CGAGATCGAG ACAGGTCTGT GAGGAGCAAG 
TCCTCAGATG ACACACCACT GCCGACCCCC TCCTATAAGT ACAACGAGTG GGCTGATGAT 
AGAAGACACT TGGGGTCTAC CCCACGTCTG TCCAGGGGCC GAGGAAGACG TGAGGATGGT 
GAAGAAGGGA TTGCATTTGA CACGGAGGAG GAGCGGCAGC AGTGGGAGGA CGACCAGAGG 
CAAGCAGACC GGGATTGGTA CATGATGGAC GAGGGCTATG ATGAGTTCCA CAACCCTCTG 
GCTTATTCCT CTGAGGACTA CGTGAGGCGG CGGGAGCAGC ACCTGCACAA ACAGAAGCAG 
AAGCGCATTT CAGCACAGCG CAGGCAGATC AATGAGGATA ATGAGCGTTG GGAGACTAAC 
CGGATGCTCA CCAGCGGGGT GGTCCATCGG CTGGAGGTGG ATGAGGACTT TGAAGAGGAC 
AACGCGGCCA AGGTGCATCT GATGGTGCAC AACCTGGTGC CTCCCTTTCT GGATGGACGG 
ATTGTTTTCA CCAAGCAGCC GGAGCCTGTG ATCCCAGTGA AGGATGCCAC CTCAGACCTG 
GCCATTATTG CTCGGAAAGG CAGCCAGACG GTCCGCAAGC ACAGGGAACA GAAAGAACGG 
AGAAAGGCTC AGCACAAACA CTGGGAACTG GCAGGGACCA AACTAGGAGA CATAATGGGT 
GTCAAAAAAG AAGAGGAGCC AGATAAAGCT ATGACTGAAG ATGGGAAAGT GGACTACAGG 
ACAGAGCAGA AGTTTGCAGA TCACATGAAG GAGAAAAGTG AGGCCAGCAG TGAGTTTGCG 
AAGAAGAAGT CGATCCTGGA GCAGAGGCAA TACCTGCCCA TCTTTGCTGT ACAGCAGGAA 
CTCCTCACCA TCATCAGAGA CAACAGCATT GTGATCGTGG TCGGGGAGAC GGGGAGTGGC 
AAGACCACTC AGCTGACCCA GTACTTGCAT CAAGATGGGT ACACGGACTA TGGGATGATC 
GGGTGCACAC AGCCCCGGCG TGTGGCTGCC ATGTCAGTGG CCAAGAGAGT CAGTGAAGAG 
ATGGGGGGAA ACCTTGGAGA AGAGGTGGGT TATGCCATTC GCTTTGAAGA CTGCACTTCT 
GAAAACACCT TGATCAAATA CATGACCGAT GGGATCCTGC TCCGAGAGTC CCTGCGGGAG 
GCTGACCTAG ACCACTACAG CGCCATCATC ATGGATGAGG CCCATGAGCG CTCCCTCAAC 
ACCGACGTGC TCTTTGGGCT GCTCCGGGAG GTTGTGGCTC GACGCTCAGA CCTGAAGCTC 
ATTGTCACCT CAGCTACTAT GGATGCAGAC AAATTTGCTG CCTTCTTTGG GAATGTACCC 
ATCTTCCACA TCCCTGGGCG AACTTTTCCT GTTGACATCC TCTTCAGCAA GACCCCACAG 
GAGGATTATG TGGAGGCCGC AGTGAAGCAG TCCTTGCAGG TGCACCTGTC TGGGGCCCCT 
GGAGACATCC TTATCTTCAT GCCTGGCCAA GAGGACATTG AGGTGACGTC TGACCAGATC 
GTAGAGCACC TAGAGGAGCT GGAGAACGCG CCTGCCTTGG CTGTGCTGCC CATCTACTCC 
CAGCTGCCGT CTGACCTTCA GGCAAAAATC TTCCAGAAGG CTCCCGATGG CGTCCGGAAG 
TGCATCGTTG CTACCAACAT TGCTGAGACG TCTCTGACTG TTGACGGCAT CATGTTTGTC 
ATCGACTCTG GCTACTGCAA GTTAAAGGTC TTCAACCCCA GGATCGGCAT GGATGCTCTG 
CAGATCTACC CCATTAGCCA GGCCAATGCC AACCAGAGGT CAGGGAGAGC TGGCAGGACG 
GGCCCAGGTC AGTGTTTCAG GCTCTACACA CAGAGCGCTT ACAAGAATGA GCTCCTGACT 
ACCACTGTGC CTGAGATCCA GAGGACCAAC CTTGCCAACG TGGTGCTGCT GCTCAAGTCC 
CTGGGGGTGC AGGACCTGCT GCAGTTCCAC TTCATGGATC CACCCCCAGA GGACAACATG 
CTCAACTCCA TGTACCAGCT GTGGATCCTC GGAGCCCTGG ATAACACAGG TGGTCTCACT 
TCCACGGGGC GGCTGATGGT GGAGTTCCCA CTGGACCCGG CTCTCTCCAA GATGCTCATC 
GTGTCTTGTG ACATGGGCTG CAGCTCCGAG ATCCTGCTCA TCGTCTCCAT GCTCTCCGTC 
CCTGCCATCT TCTACAGGCC CAAGGGCAGA GAGGAGGAGA GCGATCAGAT CCGGGAGAAG 
TTTGCTGTCC CTGAGAGTGA TCACCTGACC TACCTGAACG TCTACCTGCA GTGGAAGAAC 
AACAATTACT CCACCATTTG GTGCAACGAC CACTTCATCC ACGCCAAGGC CATGCGGAAG 
GTCCGGGAGG TGCGGGCCCA GCTCAAGGAT ATCATGGTGC AGCAGCGGAT GAGCCTGGCC 
TCATGTGGCA CTGACTGGGA CATTGTCAGG AAGTGCATCT GTGCTGCCTA TTTCCACCAG 
GCAGCCAAGC TCAAGGGCAT TGGGGAGTAT GTGAACATCC GTACAGGGAT GCCCTGCCAC 
TTACACCCCA CCAGCTCCCT CTTCGGAATG GGTTACACCC CTGACTACAT TGTATATCAC 
GAGCTGGTCA TGACCACCAA GGAGTACATG CAGTGTGTGA CCGCCGTGGA TGGAGAGTGG 
CTGGCGGAGC TGGGCCCCAT GTTCTACAGT GTGAAACAGG CAGGCAAGTC TCGGCAGGAG 
AATCGTCGTC GGGCTAAAGA GGAGGCCTCT GCCATGGAAG AGGAAATGGC CCTAGCTGAG 
GAGCAGCTGC GCGCCCGGCG GCAGGAGCAG GAGAAGCGCA GCCCGCTGGG CAGTGTCAGG 
TCTACAAAGA TCTACACTCC AGGACGGAAA GAACAGGGGG AACCCATGAC ACCTCGCCGC 
ACACCAGCCC GCTTTGGACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx38 ( XM_008772489.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770711.1 Rattus norvegicus DEAH-box helicase 38 (Dhx38), transcript variant X2, mRNA.

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CloneID	ORa38917
Clone ID Related Accession (Same CDS sequence)	XM_006255577.2 , XM_008772489.2
Accession Version	XM_008772489.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772489.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG CTGGTGAGGA CGCCTCCATC CATCGCTTGG AAGGCACCAA CTTGGACTCT 
CAGGTTGGTG GCCTTATTTG CAAGACCAAA AGTGCAGCCA GTGAGCAGCA TGTCTTCAAA 
GCCCCTGCTC CTCGCCCTTC ATTGCTGGGA CTGGACTTAC TGGCGTCCCT GAAACGGAGG 
GAGCGAGAGG AGAAGGATGA TGGGGAGGAC AAGAAAAAGT CCAGAATCTC TTCCTACAAA 
GACTGGGAGG AGAGTAAGGA TGACCAGAAG GATGCCGAGG AGGATGGCGA TGAGCAGGCT 
GGCCGAAGTA GCCGGAGAGA CAGGCACTAC CGATCTGCTC GGGTAGAGAC TCCATCCCAC 
CCCGGGGGTG TGAGTGAAGA GTTTTGGGAA CGCAGTAGGC AGAGAGAGCG GGATCGGAGG 
GAGCATGGAG TCTACGCCTC ATCCAAAGAA GACAAGGATC GGAAGAAGGA GAAATCCCGG 
GATCGAGACC ATGACCGCAA GAGAGACAGA GATGAGCGGG ACAGAAGTAG GCACAGCAGC 
AGATCAGAAC GAGACGGAGG CTCAGAGCGC AGCAGCAGAA GAAATGAACC AGAGAGTCCT 
CGACACCGCC CAAAAGATGC AGCCACCCCT TCAAGGTCCA CCTGGGAGGA GGAGGACAGT 
GGCTATGGCT CTTCAAGGCG CTCTCAGTGG GAAACACCCT CCCCAACACC TTCCTATCGG 
GACTCTGAGC GGAGTCATCG GCCGTCCACA CGAGATCGAG ACAGGTCTGT GAGGAGCAAG 
TCCTCAGATG ACACACCACT GCCGACCCCC TCCTATAAGT ACAACGAGTG GGCTGATGAT 
AGAAGACACT TGGGGTCTAC CCCACGTCTG TCCAGGGGCC GAGGAAGACG TGAGGATGGT 
GAAGAAGGGA TTGCATTTGA CACGGAGGAG GAGCGGCAGC AGTGGGAGGA CGACCAGAGG 
CAAGCAGACC GGGATTGGTA CATGATGGAC GAGGGCTATG ATGAGTTCCA CAACCCTCTG 
GCTTATTCCT CTGAGGACTA CGTGAGGCGG CGGGAGCAGC ACCTGCACAA ACAGAAGCAG 
AAGCGCATTT CAGCACAGCG CAGGCAGATC AATGAGGATA ATGAGCGTTG GGAGACTAAC 
CGGATGCTCA CCAGCGGGGT GGTCCATCGG CTGGAGGTGG ATGAGGACTT TGAAGAGGAC 
AACGCGGCCA AGGTGCATCT GATGGTGCAC AACCTGGTGC CTCCCTTTCT GGATGGACGG 
ATTGTTTTCA CCAAGCAGCC GGAGCCTGTG ATCCCAGTGA AGGATGCCAC CTCAGACCTG 
GCCATTATTG CTCGGAAAGG CAGCCAGACG GTCCGCAAGC ACAGGGAACA GAAAGAACGG 
AGAAAGGCTC AGCACAAACA CTGGGAACTG GCAGGGACCA AACTAGGAGA CATAATGGGT 
GTCAAAAAAG AAGAGGAGCC AGATAAAGCT ATGACTGAAG ATGGGAAAGT GGACTACAGG 
ACAGAGCAGA AGTTTGCAGA TCACATGAAG GAGAAAAGTG AGGCCAGCAG TGAGTTTGCG 
AAGAAGAAGT CGATCCTGGA GCAGAGGCAA TACCTGCCCA TCTTTGCTGT ACAGCAGGAA 
CTCCTCACCA TCATCAGAGA CAACAGCATT GTGATCGTGG TCGGGGAGAC GGGGAGTGGC 
AAGACCACTC AGCTGACCCA GTACTTGCAT CAAGATGGGT ACACGGACTA TGGGATGATC 
GGGTGCACAC AGCCCCGGCG TGTGGCTGCC ATGTCAGTGG CCAAGAGAGT CAGTGAAGAG 
ATGGGGGGAA ACCTTGGAGA AGAGGTGGGT TATGCCATTC GCTTTGAAGA CTGCACTTCT 
GAAAACACCT TGATCAAATA CATGACCGAT GGGATCCTGC TCCGAGAGTC CCTGCGGGAG 
GCTGACCTAG ACCACTACAG CGCCATCATC ATGGATGAGG CCCATGAGCG CTCCCTCAAC 
ACCGACGTGC TCTTTGGGCT GCTCCGGGAG GTTGTGGCTC GACGCTCAGA CCTGAAGCTC 
ATTGTCACCT CAGCTACTAT GGATGCAGAC AAATTTGCTG CCTTCTTTGG GAATGTACCC 
ATCTTCCACA TCCCTGGGCG AACTTTTCCT GTTGACATCC TCTTCAGCAA GACCCCACAG 
GAGGATTATG TGGAGGCCGC AGTGAAGCAG TCCTTGCAGG TGCACCTGTC TGGGGCCCCT 
GGAGACATCC TTATCTTCAT GCCTGGCCAA GAGGACATTG AGGTGACGTC TGACCAGATC 
GTAGAGCACC TAGAGGAGCT GGAGAACGCG CCTGCCTTGG CTGTGCTGCC CATCTACTCC 
CAGCTGCCGT CTGACCTTCA GGCAAAAATC TTCCAGAAGG CTCCCGATGG CGTCCGGAAG 
TGCATCGTTG CTACCAACAT TGCTGAGACG TCTCTGACTG TTGACGGCAT CATGTTTGTC 
ATCGACTCTG GCTACTGCAA GTTAAAGGTC TTCAACCCCA GGATCGGCAT GGATGCTCTG 
CAGATCTACC CCATTAGCCA GGCCAATGCC AACCAGAGGT CAGGGAGAGC TGGCAGGACG 
GGCCCAGGTC AGTGTTTCAG GCTCTACACA CAGAGCGCTT ACAAGAATGA GCTCCTGACT 
ACCACTGTGC CTGAGATCCA GAGGACCAAC CTTGCCAACG TGGTGCTGCT GCTCAAGTCC 
CTGGGGGTGC AGGACCTGCT GCAGTTCCAC TTCATGGATC CACCCCCAGA GGACAACATG 
CTCAACTCCA TGTACCAGCT GTGGATCCTC GGAGCCCTGG ATAACACAGG TGGTCTCACT 
TCCACGGGGC GGCTGATGGT GGAGTTCCCA CTGGACCCGG CTCTCTCCAA GATGCTCATC 
GTGTCTTGTG ACATGGGCTG CAGCTCCGAG ATCCTGCTCA TCGTCTCCAT GCTCTCCGTC 
CCTGCCATCT TCTACAGGCC CAAGGGCAGA GAGGAGGAGA GCGATCAGAT CCGGGAGAAG 
TTTGCTGTCC CTGAGAGTGA TCACCTGACC TACCTGAACG TCTACCTGCA GTGGAAGAAC 
AACAATTACT CCACCATTTG GTGCAACGAC CACTTCATCC ACGCCAAGGC CATGCGGAAG 
GTCCGGGAGG TGCGGGCCCA GCTCAAGGAT ATCATGGTGC AGCAGCGGAT GAGCCTGGCC 
TCATGTGGCA CTGACTGGGA CATTGTCAGG AAGTGCATCT GTGCTGCCTA TTTCCACCAG 
GCAGCCAAGC TCAAGGGCAT TGGGGAGTAT GTGAACATCC GTACAGGGAT GCCCTGCCAC 
TTACACCCCA CCAGCTCCCT CTTCGGAATG GGTTACACCC CTGACTACAT TGTATATCAC 
GAGCTGGTCA TGACCACCAA GGAGTACATG CAGTGTGTGA CCGCCGTGGA TGGAGAGTGG 
CTGGCGGAGC TGGGCCCCAT GTTCTACAGT GTGAAACAGG CAGGCAAGTC TCGGCAGGAG 
AATCGTCGTC GGGCTAAAGA GGAGGCCTCT GCCATGGAAG AGGAAATGGC CCTAGCTGAG 
GAGCAGCTGC GCGCCCGGCG GCAGGAGCAG GAGAAGCGCA GCCCGCTGGG CAGTGTCAGG 
TCTACAAAGA TCTACACTCC AGGACGGAAA GAACAGGGGG AACCCATGAC ACCTCGCCGC 
ACACCAGCCC GCTTTGGACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770711.1
CDS	924..4607
Translation	MEDAGEDASIHRLEGTNLDSQVGGLICKTKSAASEQHVFKAPAPRPSLLGLDLLASLKRREREEKDDGEDKKKSRISSYKDWEESKDDQKDAEEDGDEQAGRSSRRDRHYRSARVETPSHPGGVSEEFWERSRQRERDRREHGVYASSKEDKDRKKEKSRDRDHDRKRDRDERDRSRHSSRSERDGGSERSSRRNEPESPRHRPKDAATPSRSTWEEEDSGYGSSRRSQWETPSPTPSYRDSERSHRPSTRDRDRSVRSKSSDDTPLPTPSYKYNEWADDRRHLGSTPRLSRGRGRREDGEEGIAFDTEEERQQWEDDQRQADRDWYMMDEGYDEFHNPLAYSSEDYVRRREQHLHKQKQKRISAQRRQINEDNERWETNRMLTSGVVHRLEVDEDFEEDNAAKVHLMVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDLAIIARKGSQTVRKHREQKERRKAQHKHWELAGTKLGDIMGVKKEEEPDKAMTEDGKVDYRTEQKFADHMKEKSEASSEFAKKKSILEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHQDGYTDYGMIGCTQPRRVAAMSVAKRVSEEMGGNLGEEVGYAIRFEDCTSENTLIKYMTDGILLRESLREADLDHYSAIIMDEAHERSLNTDVLFGLLREVVARRSDLKLIVTSATMDADKFAAFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQSLQVHLSGAPGDILIFMPGQEDIEVTSDQIVEHLEELENAPALAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGIMFVIDSGYCKLKVFNPRIGMDALQIYPISQANANQRSGRAGRTGPGQCFRLYTQSAYKNELLTTTVPEIQRTNLANVVLLLKSLGVQDLLQFHFMDPPPEDNMLNSMYQLWILGALDNTGGLTSTGRLMVEFPLDPALSKMLIVSCDMGCSSEILLIVSMLSVPAIFYRPKGREEESDQIREKFAVPESDHLTYLNVYLQWKNNNYSTIWCNDHFIHAKAMRKVREVRAQLKDIMVQQRMSLASCGTDWDIVRKCICAAYFHQAAKLKGIGEYVNIRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSVKQAGKSRQENRRRAKEEASAMEEEMALAEEQLRARRQEQEKRSPLGSVRSTKIYTPGRKEQGEPMTPRRTPARFGL

Target ORF information:

RefSeq Version	XM_008772489.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAH-box helicase 38 (Dhx38), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772489.2

ORF Insert Sequence:

ATGGAGGACG CTGGTGAGGA CGCCTCCATC CATCGCTTGG AAGGCACCAA CTTGGACTCT 
CAGGTTGGTG GCCTTATTTG CAAGACCAAA AGTGCAGCCA GTGAGCAGCA TGTCTTCAAA 
GCCCCTGCTC CTCGCCCTTC ATTGCTGGGA CTGGACTTAC TGGCGTCCCT GAAACGGAGG 
GAGCGAGAGG AGAAGGATGA TGGGGAGGAC AAGAAAAAGT CCAGAATCTC TTCCTACAAA 
GACTGGGAGG AGAGTAAGGA TGACCAGAAG GATGCCGAGG AGGATGGCGA TGAGCAGGCT 
GGCCGAAGTA GCCGGAGAGA CAGGCACTAC CGATCTGCTC GGGTAGAGAC TCCATCCCAC 
CCCGGGGGTG TGAGTGAAGA GTTTTGGGAA CGCAGTAGGC AGAGAGAGCG GGATCGGAGG 
GAGCATGGAG TCTACGCCTC ATCCAAAGAA GACAAGGATC GGAAGAAGGA GAAATCCCGG 
GATCGAGACC ATGACCGCAA GAGAGACAGA GATGAGCGGG ACAGAAGTAG GCACAGCAGC 
AGATCAGAAC GAGACGGAGG CTCAGAGCGC AGCAGCAGAA GAAATGAACC AGAGAGTCCT 
CGACACCGCC CAAAAGATGC AGCCACCCCT TCAAGGTCCA CCTGGGAGGA GGAGGACAGT 
GGCTATGGCT CTTCAAGGCG CTCTCAGTGG GAAACACCCT CCCCAACACC TTCCTATCGG 
GACTCTGAGC GGAGTCATCG GCCGTCCACA CGAGATCGAG ACAGGTCTGT GAGGAGCAAG 
TCCTCAGATG ACACACCACT GCCGACCCCC TCCTATAAGT ACAACGAGTG GGCTGATGAT 
AGAAGACACT TGGGGTCTAC CCCACGTCTG TCCAGGGGCC GAGGAAGACG TGAGGATGGT 
GAAGAAGGGA TTGCATTTGA CACGGAGGAG GAGCGGCAGC AGTGGGAGGA CGACCAGAGG 
CAAGCAGACC GGGATTGGTA CATGATGGAC GAGGGCTATG ATGAGTTCCA CAACCCTCTG 
GCTTATTCCT CTGAGGACTA CGTGAGGCGG CGGGAGCAGC ACCTGCACAA ACAGAAGCAG 
AAGCGCATTT CAGCACAGCG CAGGCAGATC AATGAGGATA ATGAGCGTTG GGAGACTAAC 
CGGATGCTCA CCAGCGGGGT GGTCCATCGG CTGGAGGTGG ATGAGGACTT TGAAGAGGAC 
AACGCGGCCA AGGTGCATCT GATGGTGCAC AACCTGGTGC CTCCCTTTCT GGATGGACGG 
ATTGTTTTCA CCAAGCAGCC GGAGCCTGTG ATCCCAGTGA AGGATGCCAC CTCAGACCTG 
GCCATTATTG CTCGGAAAGG CAGCCAGACG GTCCGCAAGC ACAGGGAACA GAAAGAACGG 
AGAAAGGCTC AGCACAAACA CTGGGAACTG GCAGGGACCA AACTAGGAGA CATAATGGGT 
GTCAAAAAAG AAGAGGAGCC AGATAAAGCT ATGACTGAAG ATGGGAAAGT GGACTACAGG 
ACAGAGCAGA AGTTTGCAGA TCACATGAAG GAGAAAAGTG AGGCCAGCAG TGAGTTTGCG 
AAGAAGAAGT CGATCCTGGA GCAGAGGCAA TACCTGCCCA TCTTTGCTGT ACAGCAGGAA 
CTCCTCACCA TCATCAGAGA CAACAGCATT GTGATCGTGG TCGGGGAGAC GGGGAGTGGC 
AAGACCACTC AGCTGACCCA GTACTTGCAT CAAGATGGGT ACACGGACTA TGGGATGATC 
GGGTGCACAC AGCCCCGGCG TGTGGCTGCC ATGTCAGTGG CCAAGAGAGT CAGTGAAGAG 
ATGGGGGGAA ACCTTGGAGA AGAGGTGGGT TATGCCATTC GCTTTGAAGA CTGCACTTCT 
GAAAACACCT TGATCAAATA CATGACCGAT GGGATCCTGC TCCGAGAGTC CCTGCGGGAG 
GCTGACCTAG ACCACTACAG CGCCATCATC ATGGATGAGG CCCATGAGCG CTCCCTCAAC 
ACCGACGTGC TCTTTGGGCT GCTCCGGGAG GTTGTGGCTC GACGCTCAGA CCTGAAGCTC 
ATTGTCACCT CAGCTACTAT GGATGCAGAC AAATTTGCTG CCTTCTTTGG GAATGTACCC 
ATCTTCCACA TCCCTGGGCG AACTTTTCCT GTTGACATCC TCTTCAGCAA GACCCCACAG 
GAGGATTATG TGGAGGCCGC AGTGAAGCAG TCCTTGCAGG TGCACCTGTC TGGGGCCCCT 
GGAGACATCC TTATCTTCAT GCCTGGCCAA GAGGACATTG AGGTGACGTC TGACCAGATC 
GTAGAGCACC TAGAGGAGCT GGAGAACGCG CCTGCCTTGG CTGTGCTGCC CATCTACTCC 
CAGCTGCCGT CTGACCTTCA GGCAAAAATC TTCCAGAAGG CTCCCGATGG CGTCCGGAAG 
TGCATCGTTG CTACCAACAT TGCTGAGACG TCTCTGACTG TTGACGGCAT CATGTTTGTC 
ATCGACTCTG GCTACTGCAA GTTAAAGGTC TTCAACCCCA GGATCGGCAT GGATGCTCTG 
CAGATCTACC CCATTAGCCA GGCCAATGCC AACCAGAGGT CAGGGAGAGC TGGCAGGACG 
GGCCCAGGTC AGTGTTTCAG GCTCTACACA CAGAGCGCTT ACAAGAATGA GCTCCTGACT 
ACCACTGTGC CTGAGATCCA GAGGACCAAC CTTGCCAACG TGGTGCTGCT GCTCAAGTCC 
CTGGGGGTGC AGGACCTGCT GCAGTTCCAC TTCATGGATC CACCCCCAGA GGACAACATG 
CTCAACTCCA TGTACCAGCT GTGGATCCTC GGAGCCCTGG ATAACACAGG TGGTCTCACT 
TCCACGGGGC GGCTGATGGT GGAGTTCCCA CTGGACCCGG CTCTCTCCAA GATGCTCATC 
GTGTCTTGTG ACATGGGCTG CAGCTCCGAG ATCCTGCTCA TCGTCTCCAT GCTCTCCGTC 
CCTGCCATCT TCTACAGGCC CAAGGGCAGA GAGGAGGAGA GCGATCAGAT CCGGGAGAAG 
TTTGCTGTCC CTGAGAGTGA TCACCTGACC TACCTGAACG TCTACCTGCA GTGGAAGAAC 
AACAATTACT CCACCATTTG GTGCAACGAC CACTTCATCC ACGCCAAGGC CATGCGGAAG 
GTCCGGGAGG TGCGGGCCCA GCTCAAGGAT ATCATGGTGC AGCAGCGGAT GAGCCTGGCC 
TCATGTGGCA CTGACTGGGA CATTGTCAGG AAGTGCATCT GTGCTGCCTA TTTCCACCAG 
GCAGCCAAGC TCAAGGGCAT TGGGGAGTAT GTGAACATCC GTACAGGGAT GCCCTGCCAC 
TTACACCCCA CCAGCTCCCT CTTCGGAATG GGTTACACCC CTGACTACAT TGTATATCAC 
GAGCTGGTCA TGACCACCAA GGAGTACATG CAGTGTGTGA CCGCCGTGGA TGGAGAGTGG 
CTGGCGGAGC TGGGCCCCAT GTTCTACAGT GTGAAACAGG CAGGCAAGTC TCGGCAGGAG 
AATCGTCGTC GGGCTAAAGA GGAGGCCTCT GCCATGGAAG AGGAAATGGC CCTAGCTGAG 
GAGCAGCTGC GCGCCCGGCG GCAGGAGCAG GAGAAGCGCA GCCCGCTGGG CAGTGTCAGG 
TCTACAAAGA TCTACACTCC AGGACGGAAA GAACAGGGGG AACCCATGAC ACCTCGCCGC 
ACACCAGCCC GCTTTGGACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functions of the DExD/H-box proteins in nuclear pre-mRNA splicing.
Biochimica et biophysica acta1829(8)764-74(2013 Aug)
Chang TH,Tung L,Yeh FL,Chen JH,Chang SL

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.
RNA (New York, N.Y.)8(4)426-39(2002 Apr)
Jurica MS,Licklider LJ,Gygi SR,Grigorieff N,Moore MJ

Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing.
The EMBO journal17(7)2095-106(1998 Apr)
Zhou Z,Reed R

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Dhx38 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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