robo4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	robo4
Entrez Gene ID	100493397
Full Name	roundabout guidance receptor 4
Synonyms	ecsm4,mrb
General protein information	Preferred Names roundabout guidance receptor 4 Names roundabout homolog 4 roundabout, axon guidance receptor, homolog 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002937520.3	XP_002937566.2	roundabout homolog 4 isoform X1
XM_002937520.4	XP_002937566.3	roundabout homolog 4 isoform X1
XM_004916033.4	XP_004916090.2	roundabout homolog 4 isoform X3
XM_012966984.1	XP_012822438.1	roundabout homolog 4 isoform X2
XM_004916033.3	XP_004916090.1	roundabout homolog 4 isoform X3
XM_012966984.2	XP_012822438.2	roundabout homolog 4 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ROBO4	XP_508841.3
Rattus norvegicus (Norway rat)	Robo4	NP_852040.1
Homo sapiens (human)	ROBO4	NP_061928.4
Canis lupus familiaris (dog)	ROBO4	XP_005619671.1
	ROBO4	XP_002799918.1
Bos taurus (cattle)	ROBO4	NP_001092661.1
Xenopus tropicalis (tropical clawed frog)	LOC100493397	XP_002937566.2
Mus musculus (house mouse)	Robo4	NP_083059.2
Rattus norvegicus (Norway rat)	LOC100911068	XP_006242881.1

Gene Ontology

The following robo4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa62262	XM_002937520.4 Latest version!	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa62263	XM_012966984.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa62264	XM_004916033.4 Latest version!	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa13010	XM_002937520.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa13011	XM_004916033.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa35654	XM_012966984.1 Latest version!	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

robo4 ( XM_002937520.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937566.3 Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa62262
Clone ID Related Accession (Same CDS sequence)	XM_002937520.4
Accession Version	XM_002937520.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937520.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA 
CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC 
TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT 
GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA 
ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT 
TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC 
AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT 
AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT 
GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT 
GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC 
ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT 
TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA 
CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC 
GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA 
AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA 
TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA 
GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG 
GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA 
CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA 
GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA 
AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT 
GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT 
CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG 
GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC 
AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC 
TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA 
TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA 
TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG 
CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG 
ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA 
AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA 
AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC 
TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT 
AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT 
CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC 
GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC 
ATGGGGAGGC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA 
TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG 
CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA 
AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA 
CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA 
GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC 
TCTCTCCAAG TGATGGATCT TCTACCTTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT 
CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TAAGTGGTTG CAGTGTCTCA 
TCATATTCCG TGCGCAATCA AGAAAAGCAA GGCCATAAAA TCAACAGCAA GAAAAAGAGT 
CCCCCTGATA CACTGAACAT TAAACCAGCT TCAGAATGCT TCCCAATAAA TGATGATGGA 
GATAATGTGT TAACTCCAGA AGATGTGGCA CAATATCTTG AACTCAGTGA TCAAGGGCAA 
AATACAAGGC ATAAATCAGA ATATGATGCT TCCCTTCCAA GGCCTTTCTC TCCTTCTCAA 
ACATATGGCT ACATTTGCAG CCCGATACCT TCAGAAGCTG ATGTTGTTGA TGAAGATGAT 
GACCTTGATT TAGAGGACAT CGGTTCATTA AAGTCTTATC GCAAGTACTG TGAAACTCCA 
ACCTCCAGTA TCAGTGAATA TGAGAGCTCT ATGGCCGGAT CTCTTGTTAA TGGCTGGGGT 
TCTGTTTCAG AAGATAATTA TACCAGTGCT AGATGCAGCA TGGTCAGCTC CTCTGATGGG 
TCCTTTTTAA TGGATGCAAA CTTCGCCAAG GCTCTTGCAG TTGCAGTTGA CAGTTTTTGC 
TTTGGTGGAT CTCAGACTGA GACTGCTGGA AGAGATACGT TTTATGCAGA TCTTTCTCCG 
TCAGTGTCTC CTTTGGATGG AATATTAAAC CAACATAGTG GGGAAAATAC AGATGTTACC 
AAAAAGAAAT CCAGAGTTAA TCATTTGCCT GTTTTAGACT GGAACATTGA CTGGATGGAT 
GAAATGGAAG CCAAGTACAG CAGGAAAAAT GACATGAAAC ATCATTTTCC TTTTGATAAA 
AAGATGGATC CTTTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937566.3
CDS	143..3643
Translation	MNFAYFLPIFVLFPLGWCSQVSSCQCHQPVINKEHGLEATRRQTRHVQHHRSTRRHRIRGSKTRSEDFPPRIIEHPSDLFVKKDKPATLYCRAEGNPQPTIEWYRNGEHVETSTDDKTQRSYVQLPEGPLFFFSLNQRKGKSDEGIYTCVARNHLGTAFSRNASLYVAALRDDFRLNPSDAVVAVGKQLEIECLPPKGHPEPNVTWKKDGIPINHNNSRYTISAGKLFIGKALKTDSGLYSCLASNQVGERESRAAKIVVLEKPMFTYKPSDVTAKFGSTVQFGCGAQGDPKPTFHWSKENGDLPQGRYEITNENTLRIRSVTLYDSGKYICTAGNQLETVSAKALLTVQDPLDTGQIEKERQIYRELMGVRVHLDNITALPSASVYVHWQVLTESPFTEGYSVFYRTLSSTESEWTEWTKSRINEYSTVIPSLWRGQKYEFKIRPFAGKNYGPDSNIKHLRIPEEVPNIAPQNINVTAVEGGNGTIVVSWEPPPKNIHSSNIKGYKVWCFENETHPKADWIVDEETKSLEIPMLASGIKYQVQVAAINDAGIGMPSIPKYIMIDSPEVKEVKDENIYFELILQVVRHPAFIGTVGTVAWILLMVAAVYVCQRHSKRYSTKQHSILGNSLYRFASEDTIIKHRMDISDSPWLSHTWKSASCSRNYSSTISMNSQLLWSETKDTADFHKSTMSFERKSEGSRSQIIPLVPDSNSLYGTMYVDLPGKDMTTFQRLPSDKSQGMGRHFKTPDPLGLLHHRPLPSYLNDFSGSNDMNGGTRSKLPWQPNINNNPDISFKDSWSKNCKKELQHANSAPLSPYCQAPKQGDSVPSIQHLNIKKVGKGDYAKVMKTFSSPKILHYTTSLQVMDLLPYTPSLPPPPVPPPDENPIHNDKDKISGCSVSSYSVRNQEKQGHKINSKKKSPPDTLNIKPASECFPINDDGDNVLTPEDVAQYLELSDQGQNTRHKSEYDASLPRPFSPSQTYGYICSPIPSEADVVDEDDDLDLEDIGSLKSYRKYCETPTSSISEYESSMAGSLVNGWGSVSEDNYTSARCSMVSSSDGSFLMDANFAKALAVAVDSFCFGGSQTETAGRDTFYADLSPSVSPLDGILNQHSGENTDVTKKKSRVNHLPVLDWNIDWMDEMEAKYSRKNDMKHHFPFDKKMDPFK

Target ORF information:

RefSeq Version	XM_002937520.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937520.4

ORF Insert Sequence:

ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA 
CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC 
TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT 
GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA 
ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT 
TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC 
AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT 
AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT 
GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT 
GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC 
ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT 
TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA 
CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC 
GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA 
AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA 
TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA 
GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG 
GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA 
CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA 
GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA 
AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT 
GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT 
CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG 
GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC 
AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC 
TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA 
TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA 
TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG 
CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG 
ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA 
AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA 
AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC 
TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT 
AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT 
CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC 
GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC 
ATGGGGAGGC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA 
TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG 
CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA 
AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA 
CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA 
GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC 
TCTCTCCAAG TGATGGATCT TCTACCTTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT 
CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TAAGTGGTTG CAGTGTCTCA 
TCATATTCCG TGCGCAATCA AGAAAAGCAA GGCCATAAAA TCAACAGCAA GAAAAAGAGT 
CCCCCTGATA CACTGAACAT TAAACCAGCT TCAGAATGCT TCCCAATAAA TGATGATGGA 
GATAATGTGT TAACTCCAGA AGATGTGGCA CAATATCTTG AACTCAGTGA TCAAGGGCAA 
AATACAAGGC ATAAATCAGA ATATGATGCT TCCCTTCCAA GGCCTTTCTC TCCTTCTCAA 
ACATATGGCT ACATTTGCAG CCCGATACCT TCAGAAGCTG ATGTTGTTGA TGAAGATGAT 
GACCTTGATT TAGAGGACAT CGGTTCATTA AAGTCTTATC GCAAGTACTG TGAAACTCCA 
ACCTCCAGTA TCAGTGAATA TGAGAGCTCT ATGGCCGGAT CTCTTGTTAA TGGCTGGGGT 
TCTGTTTCAG AAGATAATTA TACCAGTGCT AGATGCAGCA TGGTCAGCTC CTCTGATGGG 
TCCTTTTTAA TGGATGCAAA CTTCGCCAAG GCTCTTGCAG TTGCAGTTGA CAGTTTTTGC 
TTTGGTGGAT CTCAGACTGA GACTGCTGGA AGAGATACGT TTTATGCAGA TCTTTCTCCG 
TCAGTGTCTC CTTTGGATGG AATATTAAAC CAACATAGTG GGGAAAATAC AGATGTTACC 
AAAAAGAAAT CCAGAGTTAA TCATTTGCCT GTTTTAGACT GGAACATTGA CTGGATGGAT 
GAAATGGAAG CCAAGTACAG CAGGAAAAAT GACATGAAAC ATCATTTTCC TTTTGATAAA 
AAGATGGATC CTTTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo4 ( XM_012966984.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822438.2 Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa62263
Clone ID Related Accession (Same CDS sequence)	XM_012966984.2
Accession Version	XM_012966984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA 
CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC 
TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT 
GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA 
ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT 
TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC 
AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT 
AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT 
GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT 
GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC 
ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT 
TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA 
CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC 
GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA 
AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA 
TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA 
GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG 
GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA 
CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA 
GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA 
AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT 
GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT 
CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG 
GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC 
AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC 
TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA 
TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA 
TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG 
CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG 
ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA 
AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA 
AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC 
TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT 
AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT 
CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC 
GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC 
ATGGGGAGGC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA 
TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG 
CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA 
AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA 
CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA 
GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC 
TCTCTCCAAG TGATGGATCT TCTACCTTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT 
CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TTGGTTGCAG TGTCTCATCA 
TATTCCGTGC GCAATCAAGA AAAGCAAGGC CATAAAATCA ACAGCAAGAA AAAGAGTCCC 
CCTGATACAC TGAACATTAA ACCAGCTTCA GAATGCTTCC CAATAAATGA TGATGGAGAT 
AATGTGTTAA CTCCAGAAGA TGTGGCACAA TATCTTGAAC TCAGTGATCA AGGGCAAAAT 
ACAAGGCATA AATCAGAATA TGATGCTTCC CTTCCAAGGC CTTTCTCTCC TTCTCAAACA 
TATGGCTACA TTTGCAGCCC GATACCTTCA GAAGCTGATG TTGTTGATGA AGATGATGAC 
CTTGATTTAG AGGACATCGG TTCATTAAAG TCTTATCGCA AGTACTGTGA AACTCCAACC 
TCCAGTATCA GTGAATATGA GAGCTCTATG GCCGGATCTC TTGTTAATGG CTGGGGTTCT 
GTTTCAGAAG ATAATTATAC CAGTGCTAGA TGCAGCATGG TCAGCTCCTC TGATGGGTCC 
TTTTTAATGG ATGCAAACTT CGCCAAGGCT CTTGCAGTTG CAGTTGACAG TTTTTGCTTT 
GGTGGATCTC AGACTGAGAC TGCTGGAAGA GATACGTTTT ATGCAGATCT TTCTCCGTCA 
GTGTCTCCTT TGGATGGAAT ATTAAACCAA CATAGTGGGG AAAATACAGA TGTTACCAAA 
AAGAAATCCA GAGTTAATCA TTTGCCTGTT TTAGACTGGA ACATTGACTG GATGGATGAA 
ATGGAAGCCA AGTACAGCAG GAAAAATGAC ATGAAACATC ATTTTCCTTT TGATAAAAAG 
ATGGATCCTT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822438.2
CDS	144..3641
Translation	MNFAYFLPIFVLFPLGWCSQVSSCQCHQPVINKEHGLEATRRQTRHVQHHRSTRRHRIRGSKTRSEDFPPRIIEHPSDLFVKKDKPATLYCRAEGNPQPTIEWYRNGEHVETSTDDKTQRSYVQLPEGPLFFFSLNQRKGKSDEGIYTCVARNHLGTAFSRNASLYVAALRDDFRLNPSDAVVAVGKQLEIECLPPKGHPEPNVTWKKDGIPINHNNSRYTISAGKLFIGKALKTDSGLYSCLASNQVGERESRAAKIVVLEKPMFTYKPSDVTAKFGSTVQFGCGAQGDPKPTFHWSKENGDLPQGRYEITNENTLRIRSVTLYDSGKYICTAGNQLETVSAKALLTVQDPLDTGQIEKERQIYRELMGVRVHLDNITALPSASVYVHWQVLTESPFTEGYSVFYRTLSSTESEWTEWTKSRINEYSTVIPSLWRGQKYEFKIRPFAGKNYGPDSNIKHLRIPEEVPNIAPQNINVTAVEGGNGTIVVSWEPPPKNIHSSNIKGYKVWCFENETHPKADWIVDEETKSLEIPMLASGIKYQVQVAAINDAGIGMPSIPKYIMIDSPEVKEVKDENIYFELILQVVRHPAFIGTVGTVAWILLMVAAVYVCQRHSKRYSTKQHSILGNSLYRFASEDTIIKHRMDISDSPWLSHTWKSASCSRNYSSTISMNSQLLWSETKDTADFHKSTMSFERKSEGSRSQIIPLVPDSNSLYGTMYVDLPGKDMTTFQRLPSDKSQGMGRHFKTPDPLGLLHHRPLPSYLNDFSGSNDMNGGTRSKLPWQPNINNNPDISFKDSWSKNCKKELQHANSAPLSPYCQAPKQGDSVPSIQHLNIKKVGKGDYAKVMKTFSSPKILHYTTSLQVMDLLPYTPSLPPPPVPPPDENPIHNDKDKIGCSVSSYSVRNQEKQGHKINSKKKSPPDTLNIKPASECFPINDDGDNVLTPEDVAQYLELSDQGQNTRHKSEYDASLPRPFSPSQTYGYICSPIPSEADVVDEDDDLDLEDIGSLKSYRKYCETPTSSISEYESSMAGSLVNGWGSVSEDNYTSARCSMVSSSDGSFLMDANFAKALAVAVDSFCFGGSQTETAGRDTFYADLSPSVSPLDGILNQHSGENTDVTKKKSRVNHLPVLDWNIDWMDEMEAKYSRKNDMKHHFPFDKKMDPFK

Target ORF information:

RefSeq Version	XM_012966984.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966984.2

ORF Insert Sequence:

ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA 
CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC 
TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT 
GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA 
ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT 
TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC 
AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT 
AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT 
GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT 
GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC 
ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT 
TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA 
CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC 
GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA 
AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA 
TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA 
GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG 
GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA 
CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA 
GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA 
AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT 
GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT 
CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG 
GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC 
AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC 
TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA 
TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA 
TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG 
CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG 
ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA 
AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA 
AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC 
TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT 
AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT 
CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC 
GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC 
ATGGGGAGGC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA 
TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG 
CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA 
AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA 
CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA 
GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC 
TCTCTCCAAG TGATGGATCT TCTACCTTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT 
CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TTGGTTGCAG TGTCTCATCA 
TATTCCGTGC GCAATCAAGA AAAGCAAGGC CATAAAATCA ACAGCAAGAA AAAGAGTCCC 
CCTGATACAC TGAACATTAA ACCAGCTTCA GAATGCTTCC CAATAAATGA TGATGGAGAT 
AATGTGTTAA CTCCAGAAGA TGTGGCACAA TATCTTGAAC TCAGTGATCA AGGGCAAAAT 
ACAAGGCATA AATCAGAATA TGATGCTTCC CTTCCAAGGC CTTTCTCTCC TTCTCAAACA 
TATGGCTACA TTTGCAGCCC GATACCTTCA GAAGCTGATG TTGTTGATGA AGATGATGAC 
CTTGATTTAG AGGACATCGG TTCATTAAAG TCTTATCGCA AGTACTGTGA AACTCCAACC 
TCCAGTATCA GTGAATATGA GAGCTCTATG GCCGGATCTC TTGTTAATGG CTGGGGTTCT 
GTTTCAGAAG ATAATTATAC CAGTGCTAGA TGCAGCATGG TCAGCTCCTC TGATGGGTCC 
TTTTTAATGG ATGCAAACTT CGCCAAGGCT CTTGCAGTTG CAGTTGACAG TTTTTGCTTT 
GGTGGATCTC AGACTGAGAC TGCTGGAAGA GATACGTTTT ATGCAGATCT TTCTCCGTCA 
GTGTCTCCTT TGGATGGAAT ATTAAACCAA CATAGTGGGG AAAATACAGA TGTTACCAAA 
AAGAAATCCA GAGTTAATCA TTTGCCTGTT TTAGACTGGA ACATTGACTG GATGGATGAA 
ATGGAAGCCA AGTACAGCAG GAAAAATGAC ATGAAACATC ATTTTCCTTT TGATAAAAAG 
ATGGATCCTT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo4 ( XM_004916033.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916090.2 Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X3, mRNA.

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CloneID	OXa62264
Clone ID Related Accession (Same CDS sequence)	XM_004916033.4
Accession Version	XM_004916033.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916033.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTGCA GGAGGATTTT ATCCATCATT ATAATCATTT GGACAGAAGT ACATTGGTTG 
GAAGGCTCAA AAACTCGATC AGAGGATTTT CCACCTCGTA TTATCGAACA CCCTTCAGAT 
TTGTTTGTGA AGAAGGACAA GCCAGCCACA TTGTATTGTC GAGCAGAGGG CAATCCACAG 
CCCACAATAG AATGGTACCG TAATGGAGAG CATGTGGAGA CCAGCACAGA TGATAAGACC 
CAGCGTTCCT ATGTTCAGTT GCCGGAGGGA CCACTCTTCT TCTTCAGCCT TAATCAACGA 
AAAGGCAAAT CTGATGAAGG GATTTATACT TGTGTGGCTA GAAACCATCT AGGAACGGCA 
TTTAGTAGAA ATGCTTCTCT TTATGTTGCT GCTCTAAGGG ATGATTTTCG TCTAAATCCA 
TCTGATGCTG TTGTGGCCGT GGGGAAGCAA CTTGAGATAG AATGTTTGCC TCCAAAAGGT 
CATCCTGAGC CCAATGTTAC TTGGAAGAAG GATGGGATTC CAATAAACCA TAATAATAGT 
CGATACACGA TTTCTGCAGG CAAACTCTTT ATTGGCAAAG CACTAAAGAC GGATTCAGGA 
CTATATTCCT GCTTAGCATC AAATCAAGTG GGAGAAAGAG AGAGTAGAGC TGCTAAAATC 
GTTGTACTGG AGAAGCCCAT GTTTACATAT AAACCCAGTG ATGTGACGGC AAAGTTTGGA 
AGCACCGTGC AGTTTGGTTG TGGAGCACAA GGAGACCCAA AGCCCACCTT TCACTGGAGC 
AAAGAAAATG GAGACCTTCC ACAAGGGAGG TATGAAATTA CCAATGAAAA TACATTAAGA 
ATCCGATCTG TAACCTTGTA TGACTCTGGA AAATATATCT GCACTGCAGG AAATCAGCTA 
GAAACAGTAT CTGCCAAGGC ATTGCTAACT GTTCAAGATC CTTTGGATAC TGGTCAGATA 
GAAAAGGAGA GACAAATCTA TAGAGAATTA ATGGGTGTTC GTGTTCATTT AGACAACATC 
ACAGCACTTC CAAGCGCATC AGTCTATGTG CACTGGCAGG TCCTAACGGA ATCCCCCTTT 
ACTGAAGGAT ATAGTGTATT TTACCGCACA CTTTCTTCTA CTGAATCTGA GTGGACAGAG 
TGGACAAAGT CTAGGATCAA TGAGTACAGC ACTGTTATTC CATCTCTATG GAGGGGACAG 
AAATATGAGT TTAAGATCCG ACCTTTTGCT GGGAAAAATT ATGGACCTGA CAGCAATATT 
AAACATCTCA GAATACCTGA GGAAGTTCCA AATATTGCTC CACAGAACAT CAATGTGACT 
GCTGTGGAAG GAGGAAACGG TACAATAGTT GTGAGCTGGG AACCCCCACC AAAAAACATT 
CACAGCAGTA ACATAAAAGG TTATAAGGTC TGGTGTTTTG AGAATGAAAC ACATCCTAAA 
GCAGACTGGA TAGTGGATGA AGAAACCAAG AGTCTAGAAA TTCCCATGTT GGCCTCCGGC 
ATTAAATATC AAGTCCAAGT AGCGGCTATA AATGATGCAG GAATTGGAAT GCCAAGCATA 
CCTAAATACA TAATGATAGA CTCACCAGAA GTGAAGGAGG TAAAAGATGA AAATATATAT 
TTTGAGCTAA TCCTGCAAGT TGTGCGTCAC CCAGCATTCA TTGGAACTGT AGGAACCGTG 
GCCTGGATAT TATTAATGGT TGCAGCCGTG TATGTGTGTC AGCGTCATTC TAAGCGCTAT 
AGCACAAAAC AACACAGTAT ATTAGGAAAC AGTCTTTACC GGTTTGCGAG TGAAGATACC 
ATAATAAAGC ACAGGATGGA CATTAGTGAT TCCCCATGGC TTTCACACAC ATGGAAATCC 
GCCTCCTGCT CGCGAAACTA CAGCAGCACA ATCAGTATGA ACAGCCAATT ACTGTGGTCT 
GAAACTAAAG ACACGGCAGA TTTCCATAAA TCAACAATGT CGTTTGAGCG CAAAAGTGAG 
GGTAGTCGAA GCCAGATTAT TCCGCTGGTG CCAGACAGCA ACAGCTTGTA TGGAACAATG 
TATGTCGACC TTCCAGGAAA AGATATGACC ACATTTCAGC GTCTGCCTTC TGACAAATCT 
CAAGGCATGG GGAGGCATTT TAAAACTCCT GACCCTCTAG GATTGCTTCA TCACAGGCCA 
TTGCCATCAT ATTTGAATGA TTTCAGTGGC AGCAATGATA TGAATGGAGG AACTAGAAGC 
AAGTTGCCAT GGCAACCAAA TATTAACAAT AATCCTGATA TCTCATTTAA GGACTCATGG 
AGCAAAAACT GCAAGAAAGA GCTTCAACAT GCAAATAGTG CTCCTTTGTC CCCTTATTGC 
CAAGCACCCA AACAGGGTGA CAGTGTACCA AGCATTCAGC ATTTAAACAT CAAGAAAGTA 
GGAAAAGGGG ACTATGCTAA AGTAATGAAG ACTTTCAGTT CCCCTAAAAT CCTTCACTAC 
ACCACCTCTC TCCAAGTGAT GGATCTTCTA CCTTATACAC CTTCTCTCCC ACCACCTCCA 
GTTCCTCCTC CAGATGAAAA TCCAATTCAC AATGACAAGG ATAAAATAAG TGGTTGCAGT 
GTCTCATCAT ATTCCGTGCG CAATCAAGAA AAGCAAGGCC ATAAAATCAA CAGCAAGAAA 
AAGAGTCCCC CTGATACACT GAACATTAAA CCAGCTTCAG AATGCTTCCC AATAAATGAT 
GATGGAGATA ATGTGTTAAC TCCAGAAGAT GTGGCACAAT ATCTTGAACT CAGTGATCAA 
GGGCAAAATA CAAGGCATAA ATCAGAATAT GATGCTTCCC TTCCAAGGCC TTTCTCTCCT 
TCTCAAACAT ATGGCTACAT TTGCAGCCCG ATACCTTCAG AAGCTGATGT TGTTGATGAA 
GATGATGACC TTGATTTAGA GGACATCGGT TCATTAAAGT CTTATCGCAA GTACTGTGAA 
ACTCCAACCT CCAGTATCAG TGAATATGAG AGCTCTATGG CCGGATCTCT TGTTAATGGC 
TGGGGTTCTG TTTCAGAAGA TAATTATACC AGTGCTAGAT GCAGCATGGT CAGCTCCTCT 
GATGGGTCCT TTTTAATGGA TGCAAACTTC GCCAAGGCTC TTGCAGTTGC AGTTGACAGT 
TTTTGCTTTG GTGGATCTCA GACTGAGACT GCTGGAAGAG ATACGTTTTA TGCAGATCTT 
TCTCCGTCAG TGTCTCCTTT GGATGGAATA TTAAACCAAC ATAGTGGGGA AAATACAGAT 
GTTACCAAAA AGAAATCCAG AGTTAATCAT TTGCCTGTTT TAGACTGGAA CATTGACTGG 
ATGGATGAAA TGGAAGCCAA GTACAGCAGG AAAAATGACA TGAAACATCA TTTTCCTTTT 
GATAAAAAGA TGGATCCTTT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916090.2
CDS	245..3631
Translation	MVCRRILSIIIIIWTEVHWLEGSKTRSEDFPPRIIEHPSDLFVKKDKPATLYCRAEGNPQPTIEWYRNGEHVETSTDDKTQRSYVQLPEGPLFFFSLNQRKGKSDEGIYTCVARNHLGTAFSRNASLYVAALRDDFRLNPSDAVVAVGKQLEIECLPPKGHPEPNVTWKKDGIPINHNNSRYTISAGKLFIGKALKTDSGLYSCLASNQVGERESRAAKIVVLEKPMFTYKPSDVTAKFGSTVQFGCGAQGDPKPTFHWSKENGDLPQGRYEITNENTLRIRSVTLYDSGKYICTAGNQLETVSAKALLTVQDPLDTGQIEKERQIYRELMGVRVHLDNITALPSASVYVHWQVLTESPFTEGYSVFYRTLSSTESEWTEWTKSRINEYSTVIPSLWRGQKYEFKIRPFAGKNYGPDSNIKHLRIPEEVPNIAPQNINVTAVEGGNGTIVVSWEPPPKNIHSSNIKGYKVWCFENETHPKADWIVDEETKSLEIPMLASGIKYQVQVAAINDAGIGMPSIPKYIMIDSPEVKEVKDENIYFELILQVVRHPAFIGTVGTVAWILLMVAAVYVCQRHSKRYSTKQHSILGNSLYRFASEDTIIKHRMDISDSPWLSHTWKSASCSRNYSSTISMNSQLLWSETKDTADFHKSTMSFERKSEGSRSQIIPLVPDSNSLYGTMYVDLPGKDMTTFQRLPSDKSQGMGRHFKTPDPLGLLHHRPLPSYLNDFSGSNDMNGGTRSKLPWQPNINNNPDISFKDSWSKNCKKELQHANSAPLSPYCQAPKQGDSVPSIQHLNIKKVGKGDYAKVMKTFSSPKILHYTTSLQVMDLLPYTPSLPPPPVPPPDENPIHNDKDKISGCSVSSYSVRNQEKQGHKINSKKKSPPDTLNIKPASECFPINDDGDNVLTPEDVAQYLELSDQGQNTRHKSEYDASLPRPFSPSQTYGYICSPIPSEADVVDEDDDLDLEDIGSLKSYRKYCETPTSSISEYESSMAGSLVNGWGSVSEDNYTSARCSMVSSSDGSFLMDANFAKALAVAVDSFCFGGSQTETAGRDTFYADLSPSVSPLDGILNQHSGENTDVTKKKSRVNHLPVLDWNIDWMDEMEAKYSRKNDMKHHFPFDKKMDPFK

Target ORF information:

RefSeq Version	XM_004916033.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916033.4

ORF Insert Sequence:

ATGGTTTGCA GGAGGATTTT ATCCATCATT ATAATCATTT GGACAGAAGT ACATTGGTTG 
GAAGGCTCAA AAACTCGATC AGAGGATTTT CCACCTCGTA TTATCGAACA CCCTTCAGAT 
TTGTTTGTGA AGAAGGACAA GCCAGCCACA TTGTATTGTC GAGCAGAGGG CAATCCACAG 
CCCACAATAG AATGGTACCG TAATGGAGAG CATGTGGAGA CCAGCACAGA TGATAAGACC 
CAGCGTTCCT ATGTTCAGTT GCCGGAGGGA CCACTCTTCT TCTTCAGCCT TAATCAACGA 
AAAGGCAAAT CTGATGAAGG GATTTATACT TGTGTGGCTA GAAACCATCT AGGAACGGCA 
TTTAGTAGAA ATGCTTCTCT TTATGTTGCT GCTCTAAGGG ATGATTTTCG TCTAAATCCA 
TCTGATGCTG TTGTGGCCGT GGGGAAGCAA CTTGAGATAG AATGTTTGCC TCCAAAAGGT 
CATCCTGAGC CCAATGTTAC TTGGAAGAAG GATGGGATTC CAATAAACCA TAATAATAGT 
CGATACACGA TTTCTGCAGG CAAACTCTTT ATTGGCAAAG CACTAAAGAC GGATTCAGGA 
CTATATTCCT GCTTAGCATC AAATCAAGTG GGAGAAAGAG AGAGTAGAGC TGCTAAAATC 
GTTGTACTGG AGAAGCCCAT GTTTACATAT AAACCCAGTG ATGTGACGGC AAAGTTTGGA 
AGCACCGTGC AGTTTGGTTG TGGAGCACAA GGAGACCCAA AGCCCACCTT TCACTGGAGC 
AAAGAAAATG GAGACCTTCC ACAAGGGAGG TATGAAATTA CCAATGAAAA TACATTAAGA 
ATCCGATCTG TAACCTTGTA TGACTCTGGA AAATATATCT GCACTGCAGG AAATCAGCTA 
GAAACAGTAT CTGCCAAGGC ATTGCTAACT GTTCAAGATC CTTTGGATAC TGGTCAGATA 
GAAAAGGAGA GACAAATCTA TAGAGAATTA ATGGGTGTTC GTGTTCATTT AGACAACATC 
ACAGCACTTC CAAGCGCATC AGTCTATGTG CACTGGCAGG TCCTAACGGA ATCCCCCTTT 
ACTGAAGGAT ATAGTGTATT TTACCGCACA CTTTCTTCTA CTGAATCTGA GTGGACAGAG 
TGGACAAAGT CTAGGATCAA TGAGTACAGC ACTGTTATTC CATCTCTATG GAGGGGACAG 
AAATATGAGT TTAAGATCCG ACCTTTTGCT GGGAAAAATT ATGGACCTGA CAGCAATATT 
AAACATCTCA GAATACCTGA GGAAGTTCCA AATATTGCTC CACAGAACAT CAATGTGACT 
GCTGTGGAAG GAGGAAACGG TACAATAGTT GTGAGCTGGG AACCCCCACC AAAAAACATT 
CACAGCAGTA ACATAAAAGG TTATAAGGTC TGGTGTTTTG AGAATGAAAC ACATCCTAAA 
GCAGACTGGA TAGTGGATGA AGAAACCAAG AGTCTAGAAA TTCCCATGTT GGCCTCCGGC 
ATTAAATATC AAGTCCAAGT AGCGGCTATA AATGATGCAG GAATTGGAAT GCCAAGCATA 
CCTAAATACA TAATGATAGA CTCACCAGAA GTGAAGGAGG TAAAAGATGA AAATATATAT 
TTTGAGCTAA TCCTGCAAGT TGTGCGTCAC CCAGCATTCA TTGGAACTGT AGGAACCGTG 
GCCTGGATAT TATTAATGGT TGCAGCCGTG TATGTGTGTC AGCGTCATTC TAAGCGCTAT 
AGCACAAAAC AACACAGTAT ATTAGGAAAC AGTCTTTACC GGTTTGCGAG TGAAGATACC 
ATAATAAAGC ACAGGATGGA CATTAGTGAT TCCCCATGGC TTTCACACAC ATGGAAATCC 
GCCTCCTGCT CGCGAAACTA CAGCAGCACA ATCAGTATGA ACAGCCAATT ACTGTGGTCT 
GAAACTAAAG ACACGGCAGA TTTCCATAAA TCAACAATGT CGTTTGAGCG CAAAAGTGAG 
GGTAGTCGAA GCCAGATTAT TCCGCTGGTG CCAGACAGCA ACAGCTTGTA TGGAACAATG 
TATGTCGACC TTCCAGGAAA AGATATGACC ACATTTCAGC GTCTGCCTTC TGACAAATCT 
CAAGGCATGG GGAGGCATTT TAAAACTCCT GACCCTCTAG GATTGCTTCA TCACAGGCCA 
TTGCCATCAT ATTTGAATGA TTTCAGTGGC AGCAATGATA TGAATGGAGG AACTAGAAGC 
AAGTTGCCAT GGCAACCAAA TATTAACAAT AATCCTGATA TCTCATTTAA GGACTCATGG 
AGCAAAAACT GCAAGAAAGA GCTTCAACAT GCAAATAGTG CTCCTTTGTC CCCTTATTGC 
CAAGCACCCA AACAGGGTGA CAGTGTACCA AGCATTCAGC ATTTAAACAT CAAGAAAGTA 
GGAAAAGGGG ACTATGCTAA AGTAATGAAG ACTTTCAGTT CCCCTAAAAT CCTTCACTAC 
ACCACCTCTC TCCAAGTGAT GGATCTTCTA CCTTATACAC CTTCTCTCCC ACCACCTCCA 
GTTCCTCCTC CAGATGAAAA TCCAATTCAC AATGACAAGG ATAAAATAAG TGGTTGCAGT 
GTCTCATCAT ATTCCGTGCG CAATCAAGAA AAGCAAGGCC ATAAAATCAA CAGCAAGAAA 
AAGAGTCCCC CTGATACACT GAACATTAAA CCAGCTTCAG AATGCTTCCC AATAAATGAT 
GATGGAGATA ATGTGTTAAC TCCAGAAGAT GTGGCACAAT ATCTTGAACT CAGTGATCAA 
GGGCAAAATA CAAGGCATAA ATCAGAATAT GATGCTTCCC TTCCAAGGCC TTTCTCTCCT 
TCTCAAACAT ATGGCTACAT TTGCAGCCCG ATACCTTCAG AAGCTGATGT TGTTGATGAA 
GATGATGACC TTGATTTAGA GGACATCGGT TCATTAAAGT CTTATCGCAA GTACTGTGAA 
ACTCCAACCT CCAGTATCAG TGAATATGAG AGCTCTATGG CCGGATCTCT TGTTAATGGC 
TGGGGTTCTG TTTCAGAAGA TAATTATACC AGTGCTAGAT GCAGCATGGT CAGCTCCTCT 
GATGGGTCCT TTTTAATGGA TGCAAACTTC GCCAAGGCTC TTGCAGTTGC AGTTGACAGT 
TTTTGCTTTG GTGGATCTCA GACTGAGACT GCTGGAAGAG ATACGTTTTA TGCAGATCTT 
TCTCCGTCAG TGTCTCCTTT GGATGGAATA TTAAACCAAC ATAGTGGGGA AAATACAGAT 
GTTACCAAAA AGAAATCCAG AGTTAATCAT TTGCCTGTTT TAGACTGGAA CATTGACTGG 
ATGGATGAAA TGGAAGCCAA GTACAGCAGG AAAAATGACA TGAAACATCA TTTTCCTTTT 
GATAAAAAGA TGGATCCTTT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo4 ( XM_002937520.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937566.2 Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X1, mRNA.

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CloneID	OXa13010
Clone ID Related Accession (Same CDS sequence)	XM_002937520.3
Accession Version	XM_002937520.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002937520.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA 
CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC 
TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT 
GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA 
ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT 
TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC 
AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT 
AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT 
GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT 
GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC 
ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT 
TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA 
CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC 
GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA 
AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA 
TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA 
GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG 
GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA 
CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA 
GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA 
AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT 
GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT 
CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG 
GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC 
AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC 
TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA 
TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA 
TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG 
CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG 
ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA 
AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA 
AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC 
TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT 
AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT 
CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC 
GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC 
ATGGGGAGAC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA 
TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG 
CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA 
AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA 
CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA 
GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC 
TCTCTCCAAG TGATGGATCT TCTACCCTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT 
CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TAAGTGGTTG CAGTGTCTCA 
TCATATTCCG TGCGCAATCA AGAAAAGCAA GGCCATAAAA TCAACAGCAA GAAAAAGAGT 
CCCCCTGATA CACTGAACAT TAAACCAGCT TCAGAATGCT TCCCAATAAA TGATGATGGA 
GATAATGTGT TAACTCCAGA AGATGTGGCA CAATATCTTG AACTCAGTGA TCAAGGGCAA 
AATACAAGGC ATAAATCAGA ATATGATGCT TCCCTTCCAA GGCCTTTCTC TCCTTCTCAA 
ACATATGGCT ACATTTGCAG CCCGATACCT TCAGAAGCTG ATGTTGTTGA TGAAGATGAT 
GACCTTGATT TAGAGGACAT CGGTTCATTA AAGTCTTATC GCAAGTACTG TGAAACTCCA 
ACCTCCAGTA TCAGTGAATA TGAGAGCTCT ATGGCCGGAT CTCTTGTTAA TGGCTGGGGT 
TCTGTTTCAG AAGATAATTA TACCAGTGCT AGATGCAGCA TGGTCAGCTC CTCTGATGGG 
TCCTTTTTAA TGGATGCAAA CTTCGCCAAG GCTCTTGCAG TTGCAGTTGA CAGTTTTTGC 
TTTGGTGGAT CTCAGACTGA GACTGCTGGA AGAGATACGT TTTATGCAGA TCTTTCTCCG 
TCAGTGTCTC CTTTGGATGG AATATTAAAC CAACAAAGTG GGGAAAATAC AGATGTTACC 
AAAAAGAAAT CCAGAGTTAA TCATTTGCCT GTTTTAGACT GGAACATTGA CTGGATGGAT 
GAAATGGAAG CCAGGTACAG CAGGAAAAAT GACATGAAAC ATCATTTTCC TTTTGATAAA 
AAGATGGATC CTTTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937566.2
CDS	125..3625
Translation	MNFAYFLPIFVLFPLGWCSQVSSCQCHQPVINKEHGLEATRRQTRHVQHHRSTRRHRIRGSKTRSEDFPPRIIEHPSDLFVKKDKPATLYCRAEGNPQPTIEWYRNGEHVETSTDDKTQRSYVQLPEGPLFFFSLNQRKGKSDEGIYTCVARNHLGTAFSRNASLYVAALRDDFRLNPSDAVVAVGKQLEIECLPPKGHPEPNVTWKKDGIPINHNNSRYTISAGKLFIGKALKTDSGLYSCLASNQVGERESRAAKIVVLEKPMFTYKPSDVTAKFGSTVQFGCGAQGDPKPTFHWSKENGDLPQGRYEITNENTLRIRSVTLYDSGKYICTAGNQLETVSAKALLTVQDPLDTGQIEKERQIYRELMGVRVHLDNITALPSASVYVHWQVLTESPFTEGYSVFYRTLSSTESEWTEWTKSRINEYSTVIPSLWRGQKYEFKIRPFAGKNYGPDSNIKHLRIPEEVPNIAPQNINVTAVEGGNGTIVVSWEPPPKNIHSSNIKGYKVWCFENETHPKADWIVDEETKSLEIPMLASGIKYQVQVAAINDAGIGMPSIPKYIMIDSPEVKEVKDENIYFELILQVVRHPAFIGTVGTVAWILLMVAAVYVCQRHSKRYSTKQHSILGNSLYRFASEDTIIKHRMDISDSPWLSHTWKSASCSRNYSSTISMNSQLLWSETKDTADFHKSTMSFERKSEGSRSQIIPLVPDSNSLYGTMYVDLPGKDMTTFQRLPSDKSQGMGRHFKTPDPLGLLHHRPLPSYLNDFSGSNDMNGGTRSKLPWQPNINNNPDISFKDSWSKNCKKELQHANSAPLSPYCQAPKQGDSVPSIQHLNIKKVGKGDYAKVMKTFSSPKILHYTTSLQVMDLLPYTPSLPPPPVPPPDENPIHNDKDKISGCSVSSYSVRNQEKQGHKINSKKKSPPDTLNIKPASECFPINDDGDNVLTPEDVAQYLELSDQGQNTRHKSEYDASLPRPFSPSQTYGYICSPIPSEADVVDEDDDLDLEDIGSLKSYRKYCETPTSSISEYESSMAGSLVNGWGSVSEDNYTSARCSMVSSSDGSFLMDANFAKALAVAVDSFCFGGSQTETAGRDTFYADLSPSVSPLDGILNQQSGENTDVTKKKSRVNHLPVLDWNIDWMDEMEARYSRKNDMKHHFPFDKKMDPFK

Target ORF information:

RefSeq Version	XM_002937520.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937520.3

ORF Insert Sequence:

ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA 
CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC 
TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT 
GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA 
ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT 
TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC 
AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT 
AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT 
GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT 
GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC 
ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT 
TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA 
CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC 
GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA 
AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA 
TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA 
GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG 
GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA 
CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA 
GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA 
AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT 
GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT 
CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG 
GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC 
AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC 
TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA 
TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA 
TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG 
CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG 
ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA 
AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA 
AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC 
TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT 
AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT 
CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC 
GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC 
ATGGGGAGAC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA 
TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG 
CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA 
AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA 
CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA 
GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC 
TCTCTCCAAG TGATGGATCT TCTACCCTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT 
CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TAAGTGGTTG CAGTGTCTCA 
TCATATTCCG TGCGCAATCA AGAAAAGCAA GGCCATAAAA TCAACAGCAA GAAAAAGAGT 
CCCCCTGATA CACTGAACAT TAAACCAGCT TCAGAATGCT TCCCAATAAA TGATGATGGA 
GATAATGTGT TAACTCCAGA AGATGTGGCA CAATATCTTG AACTCAGTGA TCAAGGGCAA 
AATACAAGGC ATAAATCAGA ATATGATGCT TCCCTTCCAA GGCCTTTCTC TCCTTCTCAA 
ACATATGGCT ACATTTGCAG CCCGATACCT TCAGAAGCTG ATGTTGTTGA TGAAGATGAT 
GACCTTGATT TAGAGGACAT CGGTTCATTA AAGTCTTATC GCAAGTACTG TGAAACTCCA 
ACCTCCAGTA TCAGTGAATA TGAGAGCTCT ATGGCCGGAT CTCTTGTTAA TGGCTGGGGT 
TCTGTTTCAG AAGATAATTA TACCAGTGCT AGATGCAGCA TGGTCAGCTC CTCTGATGGG 
TCCTTTTTAA TGGATGCAAA CTTCGCCAAG GCTCTTGCAG TTGCAGTTGA CAGTTTTTGC 
TTTGGTGGAT CTCAGACTGA GACTGCTGGA AGAGATACGT TTTATGCAGA TCTTTCTCCG 
TCAGTGTCTC CTTTGGATGG AATATTAAAC CAACAAAGTG GGGAAAATAC AGATGTTACC 
AAAAAGAAAT CCAGAGTTAA TCATTTGCCT GTTTTAGACT GGAACATTGA CTGGATGGAT 
GAAATGGAAG CCAGGTACAG CAGGAAAAAT GACATGAAAC ATCATTTTCC TTTTGATAAA 
AAGATGGATC CTTTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo4 ( XM_004916033.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916090.1 Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X3, mRNA.

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CloneID	OXa13011
Clone ID Related Accession (Same CDS sequence)	XM_004916033.3
Accession Version	XM_004916033.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTGCA GGAGGATTTT ATCCATCATT ATAATCATTT GGACAGAAGT ACATTGGTTG 
GAAGGCTCAA AAACTCGATC AGAGGATTTT CCACCTCGTA TTATCGAACA CCCTTCAGAT 
TTGTTTGTGA AGAAGGACAA GCCAGCCACA TTGTATTGTC GAGCAGAGGG CAATCCACAG 
CCCACAATAG AATGGTACCG TAATGGAGAG CATGTGGAGA CCAGCACAGA TGATAAGACC 
CAGCGTTCCT ATGTTCAGTT GCCGGAGGGA CCACTCTTCT TCTTCAGCCT TAATCAACGA 
AAAGGCAAAT CTGATGAAGG GATTTATACT TGTGTGGCTA GAAACCATCT AGGAACGGCA 
TTTAGTAGAA ATGCTTCTCT TTATGTTGCT GCTCTAAGGG ATGATTTTCG TCTAAATCCA 
TCTGATGCTG TTGTGGCCGT GGGGAAGCAA CTTGAGATAG AATGTTTGCC TCCAAAAGGT 
CATCCTGAGC CCAATGTTAC TTGGAAGAAG GATGGGATTC CAATAAACCA TAATAATAGT 
CGATACACGA TTTCTGCAGG CAAACTCTTT ATTGGCAAAG CACTAAAGAC GGATTCAGGA 
CTATATTCCT GCTTAGCATC AAATCAAGTG GGAGAAAGAG AGAGTAGAGC TGCTAAAATC 
GTTGTACTGG AGAAGCCCAT GTTTACATAT AAACCCAGTG ATGTGACGGC AAAGTTTGGA 
AGCACCGTGC AGTTTGGTTG TGGAGCACAA GGAGACCCAA AGCCCACCTT TCACTGGAGC 
AAAGAAAATG GAGACCTTCC ACAAGGGAGG TATGAAATTA CCAATGAAAA TACATTAAGA 
ATCCGATCTG TAACCTTGTA TGACTCTGGA AAATATATCT GCACTGCAGG AAATCAGCTA 
GAAACAGTAT CTGCCAAGGC ATTGCTAACT GTTCAAGATC CTTTGGATAC TGGTCAGATA 
GAAAAGGAGA GACAAATCTA TAGAGAATTA ATGGGTGTTC GTGTTCATTT AGACAACATC 
ACAGCACTTC CAAGCGCATC AGTCTATGTG CACTGGCAGG TCCTAACGGA ATCCCCCTTT 
ACTGAAGGAT ATAGTGTATT TTACCGCACA CTTTCTTCTA CTGAATCTGA GTGGACAGAG 
TGGACAAAGT CTAGGATCAA TGAGTACAGC ACTGTTATTC CATCTCTATG GAGGGGACAG 
AAATATGAGT TTAAGATCCG ACCTTTTGCT GGGAAAAATT ATGGACCTGA CAGCAATATT 
AAACATCTCA GAATACCTGA GGAAGTTCCA AATATTGCTC CACAGAACAT CAATGTGACT 
GCTGTGGAAG GAGGAAACGG TACAATAGTT GTGAGCTGGG AACCCCCACC AAAAAACATT 
CACAGCAGTA ACATAAAAGG TTATAAGGTC TGGTGTTTTG AGAATGAAAC ACATCCTAAA 
GCAGACTGGA TAGTGGATGA AGAAACCAAG AGTCTAGAAA TTCCCATGTT GGCCTCCGGC 
ATTAAATATC AAGTCCAAGT AGCGGCTATA AATGATGCAG GAATTGGAAT GCCAAGCATA 
CCTAAATACA TAATGATAGA CTCACCAGAA GTGAAGGAGG TAAAAGATGA AAATATATAT 
TTTGAGCTAA TCCTGCAAGT TGTGCGTCAC CCAGCATTCA TTGGAACTGT AGGAACCGTG 
GCCTGGATAT TATTAATGGT TGCAGCCGTG TATGTGTGTC AGCGTCATTC TAAGCGCTAT 
AGCACAAAAC AACACAGTAT ATTAGGAAAC AGTCTTTACC GGTTTGCGAG TGAAGATACC 
ATAATAAAGC ACAGGATGGA CATTAGTGAT TCCCCATGGC TTTCACACAC ATGGAAATCC 
GCCTCCTGCT CGCGAAACTA CAGCAGCACA ATCAGTATGA ACAGCCAATT ACTGTGGTCT 
GAAACTAAAG ACACGGCAGA TTTCCATAAA TCAACAATGT CGTTTGAGCG CAAAAGTGAG 
GGTAGTCGAA GCCAGATTAT TCCGCTGGTG CCAGACAGCA ACAGCTTGTA TGGAACAATG 
TATGTCGACC TTCCAGGAAA AGATATGACC ACATTTCAGC GTCTGCCTTC TGACAAATCT 
CAAGGCATGG GGAGACATTT TAAAACTCCT GACCCTCTAG GATTGCTTCA TCACAGGCCA 
TTGCCATCAT ATTTGAATGA TTTCAGTGGC AGCAATGATA TGAATGGAGG AACTAGAAGC 
AAGTTGCCAT GGCAACCAAA TATTAACAAT AATCCTGATA TCTCATTTAA GGACTCATGG 
AGCAAAAACT GCAAGAAAGA GCTTCAACAT GCAAATAGTG CTCCTTTGTC CCCTTATTGC 
CAAGCACCCA AACAGGGTGA CAGTGTACCA AGCATTCAGC ATTTAAACAT CAAGAAAGTA 
GGAAAAGGGG ACTATGCTAA AGTAATGAAG ACTTTCAGTT CCCCTAAAAT CCTTCACTAC 
ACCACCTCTC TCCAAGTGAT GGATCTTCTA CCCTATACAC CTTCTCTCCC ACCACCTCCA 
GTTCCTCCTC CAGATGAAAA TCCAATTCAC AATGACAAGG ATAAAATAAG TGGTTGCAGT 
GTCTCATCAT ATTCCGTGCG CAATCAAGAA AAGCAAGGCC ATAAAATCAA CAGCAAGAAA 
AAGAGTCCCC CTGATACACT GAACATTAAA CCAGCTTCAG AATGCTTCCC AATAAATGAT 
GATGGAGATA ATGTGTTAAC TCCAGAAGAT GTGGCACAAT ATCTTGAACT CAGTGATCAA 
GGGCAAAATA CAAGGCATAA ATCAGAATAT GATGCTTCCC TTCCAAGGCC TTTCTCTCCT 
TCTCAAACAT ATGGCTACAT TTGCAGCCCG ATACCTTCAG AAGCTGATGT TGTTGATGAA 
GATGATGACC TTGATTTAGA GGACATCGGT TCATTAAAGT CTTATCGCAA GTACTGTGAA 
ACTCCAACCT CCAGTATCAG TGAATATGAG AGCTCTATGG CCGGATCTCT TGTTAATGGC 
TGGGGTTCTG TTTCAGAAGA TAATTATACC AGTGCTAGAT GCAGCATGGT CAGCTCCTCT 
GATGGGTCCT TTTTAATGGA TGCAAACTTC GCCAAGGCTC TTGCAGTTGC AGTTGACAGT 
TTTTGCTTTG GTGGATCTCA GACTGAGACT GCTGGAAGAG ATACGTTTTA TGCAGATCTT 
TCTCCGTCAG TGTCTCCTTT GGATGGAATA TTAAACCAAC AAAGTGGGGA AAATACAGAT 
GTTACCAAAA AGAAATCCAG AGTTAATCAT TTGCCTGTTT TAGACTGGAA CATTGACTGG 
ATGGATGAAA TGGAAGCCAG GTACAGCAGG AAAAATGACA TGAAACATCA TTTTCCTTTT 
GATAAAAAGA TGGATCCTTT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916090.1
CDS	246..3632
Translation	MVCRRILSIIIIIWTEVHWLEGSKTRSEDFPPRIIEHPSDLFVKKDKPATLYCRAEGNPQPTIEWYRNGEHVETSTDDKTQRSYVQLPEGPLFFFSLNQRKGKSDEGIYTCVARNHLGTAFSRNASLYVAALRDDFRLNPSDAVVAVGKQLEIECLPPKGHPEPNVTWKKDGIPINHNNSRYTISAGKLFIGKALKTDSGLYSCLASNQVGERESRAAKIVVLEKPMFTYKPSDVTAKFGSTVQFGCGAQGDPKPTFHWSKENGDLPQGRYEITNENTLRIRSVTLYDSGKYICTAGNQLETVSAKALLTVQDPLDTGQIEKERQIYRELMGVRVHLDNITALPSASVYVHWQVLTESPFTEGYSVFYRTLSSTESEWTEWTKSRINEYSTVIPSLWRGQKYEFKIRPFAGKNYGPDSNIKHLRIPEEVPNIAPQNINVTAVEGGNGTIVVSWEPPPKNIHSSNIKGYKVWCFENETHPKADWIVDEETKSLEIPMLASGIKYQVQVAAINDAGIGMPSIPKYIMIDSPEVKEVKDENIYFELILQVVRHPAFIGTVGTVAWILLMVAAVYVCQRHSKRYSTKQHSILGNSLYRFASEDTIIKHRMDISDSPWLSHTWKSASCSRNYSSTISMNSQLLWSETKDTADFHKSTMSFERKSEGSRSQIIPLVPDSNSLYGTMYVDLPGKDMTTFQRLPSDKSQGMGRHFKTPDPLGLLHHRPLPSYLNDFSGSNDMNGGTRSKLPWQPNINNNPDISFKDSWSKNCKKELQHANSAPLSPYCQAPKQGDSVPSIQHLNIKKVGKGDYAKVMKTFSSPKILHYTTSLQVMDLLPYTPSLPPPPVPPPDENPIHNDKDKISGCSVSSYSVRNQEKQGHKINSKKKSPPDTLNIKPASECFPINDDGDNVLTPEDVAQYLELSDQGQNTRHKSEYDASLPRPFSPSQTYGYICSPIPSEADVVDEDDDLDLEDIGSLKSYRKYCETPTSSISEYESSMAGSLVNGWGSVSEDNYTSARCSMVSSSDGSFLMDANFAKALAVAVDSFCFGGSQTETAGRDTFYADLSPSVSPLDGILNQQSGENTDVTKKKSRVNHLPVLDWNIDWMDEMEARYSRKNDMKHHFPFDKKMDPFK

Target ORF information:

RefSeq Version	XM_004916033.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916033.3

ORF Insert Sequence:

ATGGTTTGCA GGAGGATTTT ATCCATCATT ATAATCATTT GGACAGAAGT ACATTGGTTG 
GAAGGCTCAA AAACTCGATC AGAGGATTTT CCACCTCGTA TTATCGAACA CCCTTCAGAT 
TTGTTTGTGA AGAAGGACAA GCCAGCCACA TTGTATTGTC GAGCAGAGGG CAATCCACAG 
CCCACAATAG AATGGTACCG TAATGGAGAG CATGTGGAGA CCAGCACAGA TGATAAGACC 
CAGCGTTCCT ATGTTCAGTT GCCGGAGGGA CCACTCTTCT TCTTCAGCCT TAATCAACGA 
AAAGGCAAAT CTGATGAAGG GATTTATACT TGTGTGGCTA GAAACCATCT AGGAACGGCA 
TTTAGTAGAA ATGCTTCTCT TTATGTTGCT GCTCTAAGGG ATGATTTTCG TCTAAATCCA 
TCTGATGCTG TTGTGGCCGT GGGGAAGCAA CTTGAGATAG AATGTTTGCC TCCAAAAGGT 
CATCCTGAGC CCAATGTTAC TTGGAAGAAG GATGGGATTC CAATAAACCA TAATAATAGT 
CGATACACGA TTTCTGCAGG CAAACTCTTT ATTGGCAAAG CACTAAAGAC GGATTCAGGA 
CTATATTCCT GCTTAGCATC AAATCAAGTG GGAGAAAGAG AGAGTAGAGC TGCTAAAATC 
GTTGTACTGG AGAAGCCCAT GTTTACATAT AAACCCAGTG ATGTGACGGC AAAGTTTGGA 
AGCACCGTGC AGTTTGGTTG TGGAGCACAA GGAGACCCAA AGCCCACCTT TCACTGGAGC 
AAAGAAAATG GAGACCTTCC ACAAGGGAGG TATGAAATTA CCAATGAAAA TACATTAAGA 
ATCCGATCTG TAACCTTGTA TGACTCTGGA AAATATATCT GCACTGCAGG AAATCAGCTA 
GAAACAGTAT CTGCCAAGGC ATTGCTAACT GTTCAAGATC CTTTGGATAC TGGTCAGATA 
GAAAAGGAGA GACAAATCTA TAGAGAATTA ATGGGTGTTC GTGTTCATTT AGACAACATC 
ACAGCACTTC CAAGCGCATC AGTCTATGTG CACTGGCAGG TCCTAACGGA ATCCCCCTTT 
ACTGAAGGAT ATAGTGTATT TTACCGCACA CTTTCTTCTA CTGAATCTGA GTGGACAGAG 
TGGACAAAGT CTAGGATCAA TGAGTACAGC ACTGTTATTC CATCTCTATG GAGGGGACAG 
AAATATGAGT TTAAGATCCG ACCTTTTGCT GGGAAAAATT ATGGACCTGA CAGCAATATT 
AAACATCTCA GAATACCTGA GGAAGTTCCA AATATTGCTC CACAGAACAT CAATGTGACT 
GCTGTGGAAG GAGGAAACGG TACAATAGTT GTGAGCTGGG AACCCCCACC AAAAAACATT 
CACAGCAGTA ACATAAAAGG TTATAAGGTC TGGTGTTTTG AGAATGAAAC ACATCCTAAA 
GCAGACTGGA TAGTGGATGA AGAAACCAAG AGTCTAGAAA TTCCCATGTT GGCCTCCGGC 
ATTAAATATC AAGTCCAAGT AGCGGCTATA AATGATGCAG GAATTGGAAT GCCAAGCATA 
CCTAAATACA TAATGATAGA CTCACCAGAA GTGAAGGAGG TAAAAGATGA AAATATATAT 
TTTGAGCTAA TCCTGCAAGT TGTGCGTCAC CCAGCATTCA TTGGAACTGT AGGAACCGTG 
GCCTGGATAT TATTAATGGT TGCAGCCGTG TATGTGTGTC AGCGTCATTC TAAGCGCTAT 
AGCACAAAAC AACACAGTAT ATTAGGAAAC AGTCTTTACC GGTTTGCGAG TGAAGATACC 
ATAATAAAGC ACAGGATGGA CATTAGTGAT TCCCCATGGC TTTCACACAC ATGGAAATCC 
GCCTCCTGCT CGCGAAACTA CAGCAGCACA ATCAGTATGA ACAGCCAATT ACTGTGGTCT 
GAAACTAAAG ACACGGCAGA TTTCCATAAA TCAACAATGT CGTTTGAGCG CAAAAGTGAG 
GGTAGTCGAA GCCAGATTAT TCCGCTGGTG CCAGACAGCA ACAGCTTGTA TGGAACAATG 
TATGTCGACC TTCCAGGAAA AGATATGACC ACATTTCAGC GTCTGCCTTC TGACAAATCT 
CAAGGCATGG GGAGACATTT TAAAACTCCT GACCCTCTAG GATTGCTTCA TCACAGGCCA 
TTGCCATCAT ATTTGAATGA TTTCAGTGGC AGCAATGATA TGAATGGAGG AACTAGAAGC 
AAGTTGCCAT GGCAACCAAA TATTAACAAT AATCCTGATA TCTCATTTAA GGACTCATGG 
AGCAAAAACT GCAAGAAAGA GCTTCAACAT GCAAATAGTG CTCCTTTGTC CCCTTATTGC 
CAAGCACCCA AACAGGGTGA CAGTGTACCA AGCATTCAGC ATTTAAACAT CAAGAAAGTA 
GGAAAAGGGG ACTATGCTAA AGTAATGAAG ACTTTCAGTT CCCCTAAAAT CCTTCACTAC 
ACCACCTCTC TCCAAGTGAT GGATCTTCTA CCCTATACAC CTTCTCTCCC ACCACCTCCA 
GTTCCTCCTC CAGATGAAAA TCCAATTCAC AATGACAAGG ATAAAATAAG TGGTTGCAGT 
GTCTCATCAT ATTCCGTGCG CAATCAAGAA AAGCAAGGCC ATAAAATCAA CAGCAAGAAA 
AAGAGTCCCC CTGATACACT GAACATTAAA CCAGCTTCAG AATGCTTCCC AATAAATGAT 
GATGGAGATA ATGTGTTAAC TCCAGAAGAT GTGGCACAAT ATCTTGAACT CAGTGATCAA 
GGGCAAAATA CAAGGCATAA ATCAGAATAT GATGCTTCCC TTCCAAGGCC TTTCTCTCCT 
TCTCAAACAT ATGGCTACAT TTGCAGCCCG ATACCTTCAG AAGCTGATGT TGTTGATGAA 
GATGATGACC TTGATTTAGA GGACATCGGT TCATTAAAGT CTTATCGCAA GTACTGTGAA 
ACTCCAACCT CCAGTATCAG TGAATATGAG AGCTCTATGG CCGGATCTCT TGTTAATGGC 
TGGGGTTCTG TTTCAGAAGA TAATTATACC AGTGCTAGAT GCAGCATGGT CAGCTCCTCT 
GATGGGTCCT TTTTAATGGA TGCAAACTTC GCCAAGGCTC TTGCAGTTGC AGTTGACAGT 
TTTTGCTTTG GTGGATCTCA GACTGAGACT GCTGGAAGAG ATACGTTTTA TGCAGATCTT 
TCTCCGTCAG TGTCTCCTTT GGATGGAATA TTAAACCAAC AAAGTGGGGA AAATACAGAT 
GTTACCAAAA AGAAATCCAG AGTTAATCAT TTGCCTGTTT TAGACTGGAA CATTGACTGG 
ATGGATGAAA TGGAAGCCAG GTACAGCAGG AAAAATGACA TGAAACATCA TTTTCCTTTT 
GATAAAAAGA TGGATCCTTT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo4 ( XM_012966984.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822438.1 Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X2, mRNA.

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CloneID	OXa35654
Clone ID Related Accession (Same CDS sequence)	XM_012966984.1
Accession Version	XM_012966984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA 
CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC 
TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT 
GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA 
ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT 
TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC 
AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT 
AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT 
GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT 
GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC 
ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT 
TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA 
CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC 
GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA 
AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA 
TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA 
GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG 
GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA 
CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA 
GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA 
AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT 
GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT 
CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG 
GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC 
AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC 
TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA 
TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA 
TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG 
CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG 
ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA 
AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA 
AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC 
TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT 
AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT 
CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC 
GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC 
ATGGGGAGAC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA 
TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG 
CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA 
AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA 
CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA 
GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC 
TCTCTCCAAG TGATGGATCT TCTACCCTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT 
CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TTGGTTGCAG TGTCTCATCA 
TATTCCGTGC GCAATCAAGA AAAGCAAGGC CATAAAATCA ACAGCAAGAA AAAGAGTCCC 
CCTGATACAC TGAACATTAA ACCAGCTTCA GAATGCTTCC CAATAAATGA TGATGGAGAT 
AATGTGTTAA CTCCAGAAGA TGTGGCACAA TATCTTGAAC TCAGTGATCA AGGGCAAAAT 
ACAAGGCATA AATCAGAATA TGATGCTTCC CTTCCAAGGC CTTTCTCTCC TTCTCAAACA 
TATGGCTACA TTTGCAGCCC GATACCTTCA GAAGCTGATG TTGTTGATGA AGATGATGAC 
CTTGATTTAG AGGACATCGG TTCATTAAAG TCTTATCGCA AGTACTGTGA AACTCCAACC 
TCCAGTATCA GTGAATATGA GAGCTCTATG GCCGGATCTC TTGTTAATGG CTGGGGTTCT 
GTTTCAGAAG ATAATTATAC CAGTGCTAGA TGCAGCATGG TCAGCTCCTC TGATGGGTCC 
TTTTTAATGG ATGCAAACTT CGCCAAGGCT CTTGCAGTTG CAGTTGACAG TTTTTGCTTT 
GGTGGATCTC AGACTGAGAC TGCTGGAAGA GATACGTTTT ATGCAGATCT TTCTCCGTCA 
GTGTCTCCTT TGGATGGAAT ATTAAACCAA CAAAGTGGGG AAAATACAGA TGTTACCAAA 
AAGAAATCCA GAGTTAATCA TTTGCCTGTT TTAGACTGGA ACATTGACTG GATGGATGAA 
ATGGAAGCCA GGTACAGCAG GAAAAATGAC ATGAAACATC ATTTTCCTTT TGATAAAAAG 
ATGGATCCTT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822438.1
CDS	125..3622
Translation	MNFAYFLPIFVLFPLGWCSQVSSCQCHQPVINKEHGLEATRRQTRHVQHHRSTRRHRIRGSKTRSEDFPPRIIEHPSDLFVKKDKPATLYCRAEGNPQPTIEWYRNGEHVETSTDDKTQRSYVQLPEGPLFFFSLNQRKGKSDEGIYTCVARNHLGTAFSRNASLYVAALRDDFRLNPSDAVVAVGKQLEIECLPPKGHPEPNVTWKKDGIPINHNNSRYTISAGKLFIGKALKTDSGLYSCLASNQVGERESRAAKIVVLEKPMFTYKPSDVTAKFGSTVQFGCGAQGDPKPTFHWSKENGDLPQGRYEITNENTLRIRSVTLYDSGKYICTAGNQLETVSAKALLTVQDPLDTGQIEKERQIYRELMGVRVHLDNITALPSASVYVHWQVLTESPFTEGYSVFYRTLSSTESEWTEWTKSRINEYSTVIPSLWRGQKYEFKIRPFAGKNYGPDSNIKHLRIPEEVPNIAPQNINVTAVEGGNGTIVVSWEPPPKNIHSSNIKGYKVWCFENETHPKADWIVDEETKSLEIPMLASGIKYQVQVAAINDAGIGMPSIPKYIMIDSPEVKEVKDENIYFELILQVVRHPAFIGTVGTVAWILLMVAAVYVCQRHSKRYSTKQHSILGNSLYRFASEDTIIKHRMDISDSPWLSHTWKSASCSRNYSSTISMNSQLLWSETKDTADFHKSTMSFERKSEGSRSQIIPLVPDSNSLYGTMYVDLPGKDMTTFQRLPSDKSQGMGRHFKTPDPLGLLHHRPLPSYLNDFSGSNDMNGGTRSKLPWQPNINNNPDISFKDSWSKNCKKELQHANSAPLSPYCQAPKQGDSVPSIQHLNIKKVGKGDYAKVMKTFSSPKILHYTTSLQVMDLLPYTPSLPPPPVPPPDENPIHNDKDKIGCSVSSYSVRNQEKQGHKINSKKKSPPDTLNIKPASECFPINDDGDNVLTPEDVAQYLELSDQGQNTRHKSEYDASLPRPFSPSQTYGYICSPIPSEADVVDEDDDLDLEDIGSLKSYRKYCETPTSSISEYESSMAGSLVNGWGSVSEDNYTSARCSMVSSSDGSFLMDANFAKALAVAVDSFCFGGSQTETAGRDTFYADLSPSVSPLDGILNQQSGENTDVTKKKSRVNHLPVLDWNIDWMDEMEARYSRKNDMKHHFPFDKKMDPFK

Target ORF information:

RefSeq Version	XM_012966984.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966984.1

ORF Insert Sequence:

ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA 
CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC 
TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT 
GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA 
ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT 
TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC 
AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT 
AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT 
GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT 
GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC 
ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT 
TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA 
CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC 
GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA 
AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA 
TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA 
GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG 
GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA 
CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA 
GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA 
AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT 
GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT 
CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG 
GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC 
AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC 
TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA 
TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA 
TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG 
CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG 
ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA 
AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA 
AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC 
TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT 
AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT 
CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC 
GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC 
ATGGGGAGAC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA 
TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG 
CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA 
AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA 
CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA 
GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC 
TCTCTCCAAG TGATGGATCT TCTACCCTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT 
CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TTGGTTGCAG TGTCTCATCA 
TATTCCGTGC GCAATCAAGA AAAGCAAGGC CATAAAATCA ACAGCAAGAA AAAGAGTCCC 
CCTGATACAC TGAACATTAA ACCAGCTTCA GAATGCTTCC CAATAAATGA TGATGGAGAT 
AATGTGTTAA CTCCAGAAGA TGTGGCACAA TATCTTGAAC TCAGTGATCA AGGGCAAAAT 
ACAAGGCATA AATCAGAATA TGATGCTTCC CTTCCAAGGC CTTTCTCTCC TTCTCAAACA 
TATGGCTACA TTTGCAGCCC GATACCTTCA GAAGCTGATG TTGTTGATGA AGATGATGAC 
CTTGATTTAG AGGACATCGG TTCATTAAAG TCTTATCGCA AGTACTGTGA AACTCCAACC 
TCCAGTATCA GTGAATATGA GAGCTCTATG GCCGGATCTC TTGTTAATGG CTGGGGTTCT 
GTTTCAGAAG ATAATTATAC CAGTGCTAGA TGCAGCATGG TCAGCTCCTC TGATGGGTCC 
TTTTTAATGG ATGCAAACTT CGCCAAGGCT CTTGCAGTTG CAGTTGACAG TTTTTGCTTT 
GGTGGATCTC AGACTGAGAC TGCTGGAAGA GATACGTTTT ATGCAGATCT TTCTCCGTCA 
GTGTCTCCTT TGGATGGAAT ATTAAACCAA CAAAGTGGGG AAAATACAGA TGTTACCAAA 
AAGAAATCCA GAGTTAATCA TTTGCCTGTT TTAGACTGGA ACATTGACTG GATGGATGAA 
ATGGAAGCCA GGTACAGCAG GAAAAATGAC ATGAAACATC ATTTTCCTTT TGATAAAAAG 
ATGGATCCTT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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robo4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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