afdn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following afdn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the afdn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa58611 XM_031902292.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58612 XM_031902293.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58613 XM_031902294.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58614 XM_031902295.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58615 XM_031902296.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58616 XM_031902297.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58617 XM_031902298.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58618 XM_031902299.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58619 XM_031902300.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58620 XM_031902301.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58621 XM_031902302.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58622 XM_031902303.1
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa35546 XM_012963753.2
Latest version!
Xenopus tropicalis afadin, adherens junction formation factor (afdn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa58611
    Clone ID Related Accession (Same CDS sequence) XM_031902292.1
    Accession Version XM_031902292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGCAGACC
    GCTATGCCAG CAATCTCTAT TCTAGATTTG TTACAGGATG AAGAACGGAG ACGGCAACAA
    CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT
    CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA
    GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA
    TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT
    TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC
    CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT
    GATGATGAGG AAGACACAAA CCTATCAGGT CAGGATAAGT ACTCCAGCAT GAGAAAATCT
    TATGGTGACC TCCCTCCAGC TGCTCATAAA CCACAGCTGC CTGCTGCACG GCTGCCTCGT
    CCTGTATCCG ATGGCATCTT TCTTTCCGGC TCATTTCAGC CCCCTTCAGC CAATGCCAAC
    AGTACTGCTT CCAAAACGGG TCCACCTATA GCAAATAAAC CAAGTTTCCT CCATTACAGC
    AGCTCCAAAG GACCAAATTC TTATTCTGGA TCATCTGGTG CAGCAGTTCC TGGTAATGAA
    GTTTATAAAG ATCCGAGGGA TGTGCGACTA AAAAGCCAAG ATGGAGAAGC TTATAGCACT
    GGTGGAGCCC CAGAGAGCCT AACCTTTAAG GAGCGCCAGC GGCTCTTCTC TCAGGGCCAG
    GATGTGTCTA ACAAAGTGAA AGCTTCCAGA AAATTAATGG AACTGGAAAA TGAGTTAAAC
    ACAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758152.1
    CDS999..6707
    Translation

    Target ORF information:

    RefSeq Version XM_031902292.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGCAGACC
    GCTATGCCAG CAATCTCTAT TCTAGATTTG TTACAGGATG AAGAACGGAG ACGGCAACAA
    CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT
    CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA
    GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA
    TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT
    TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC
    CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT
    GATGATGAGG AAGACACAAA CCTATCAGGT CAGGATAAGT ACTCCAGCAT GAGAAAATCT
    TATGGTGACC TCCCTCCAGC TGCTCATAAA CCACAGCTGC CTGCTGCACG GCTGCCTCGT
    CCTGTATCCG ATGGCATCTT TCTTTCCGGC TCATTTCAGC CCCCTTCAGC CAATGCCAAC
    AGTACTGCTT CCAAAACGGG TCCACCTATA GCAAATAAAC CAAGTTTCCT CCATTACAGC
    AGCTCCAAAG GACCAAATTC TTATTCTGGA TCATCTGGTG CAGCAGTTCC TGGTAATGAA
    GTTTATAAAG ATCCGAGGGA TGTGCGACTA AAAAGCCAAG ATGGAGAAGC TTATAGCACT
    GGTGGAGCCC CAGAGAGCCT AACCTTTAAG GAGCGCCAGC GGCTCTTCTC TCAGGGCCAG
    GATGTGTCTA ACAAAGTGAA AGCTTCCAGA AAATTAATGG AACTGGAAAA TGAGTTAAAC
    ACAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58612
    Clone ID Related Accession (Same CDS sequence) XM_031902293.1
    Accession Version XM_031902293.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT
    CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA
    AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG
    TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA
    GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG
    GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT
    ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT
    GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA
    CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT
    TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA
    TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA
    GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG
    TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG
    GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT
    TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT
    TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG
    TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA
    GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT
    ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT
    GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA
    AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT
    GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA
    GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA
    CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT
    ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC
    AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT
    GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT
    GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGGAAAT AGACAAAGGT
    AACCAGAAGC AAAAGAATAT TGCTGGAGCT CTTGCTTTCT GGATGGCAAA CGCTTCAGAG
    CTTCTCAATT TCATAAAGCA AGACCGAGAT CTCAATCGTA TCACATTGGA TGCACAAGAT
    GTTCTTGCAC ATTTAGTTCA GATGGCATTC AAATACCTGG TTCACTGTCT TCAATCTGAA
    TTGACCAGCT CTATGCCTGC TTTTATAGAT GATCCAGAAG AAAATGATTC CCAAAGGCCT
    AAAATAGAAG ACGTTTTGCA TACTCTGACA GGTGCCATGT CACTTCTAAG ACGTTGCCGG
    GTCAACGCAG CACTAACTAT TCAGCTGTTC TCGCAGCTAT TCCATTTCAT CAACATGTGG
    CTGTTCAACA GGCTTGTAAC AGATCCAGAC TCTGGTTTAT GCTCTCACTA TTGGGGGGCA
    ATAATTCGGC AGCAGCTTGC ACACATTGAG GCATGGGCTG AAAAACAAGG CCTCGAATTA
    GCTGCAGATT GTCATCTTAG CAGAATAGTT CAGGCTACTA CTCTTCTCAC TATGGACAAG
    TACTCGCATC AGGATATTCC AAATATTAAT AATACATGTT TTAAGCTGAA TTCCTTACAA
    CTGCAAGCCC TATTGCAAAA TTACCACTGT GCTCCTGATG AACCGTACAT CCCAACGGAG
    TTGATAGACA GTGTGGTGTC TGTAGCTGAA AACACTGCAG ATGAATTGGC TCGAAGTGAT
    GGCAGAGAAG TTCAGCTTGA GGAGGATCCT GATCTGCAAC TCCCTTTCCT TTTGCCTGAA
    GATGGTTATT CATGTGATGT GGTTAGAAAT ATTCCTAATG GCCTTCAAGA GTTTTTAGAA
    CCGCTCTGCC AGAGAGGTTT TTGCAGACTT GTCCCTCACT CACGGTCACC AGGCATGTGG
    ACAATTTACT TTGAAGGAGC TGAATTTGAA AATAATTTGT TGACCGACAA TACAGACCTG
    CCATTAAGAA AAGAACCTGA AATAATCACT GTGACACTTA AAAAGCAGAA TGGAATGGGT
    TTGAGTATTG TTGCAGCAAA GGGTGCTGGA CAAGAAAAAC TTGGGATTTA CATTAAGTCT
    GTGGTGAAAG GCGGTGCTGC AGAAGTGGAT GGTCGTTTAG CTGCAGGGGA CCAACTTATA
    AGTGTGGATG GGCAAAGTTT AGTTGGTTTG TCCCAAGAAA GAGCTGCAGA GCTTATGACT
    AGAACTGGGG CCGTAGTAAC CCTTGAGGTC GCAAAGCAAG GCGCAATATA CCATGGACTT
    GCCACACTAC TAAACCAACC ATCTCCTCTC ATGCAAAGAG ATCGACGTGG CTCAGGAAAA
    CCACGACCAA AGAGTGAAGG TTTTGAGCTT TACAATAATG CTTCCCAAAA TGGGTCTCCT
    GATAGCCCCC AGACACAATG GCCAGAATAC ACCGAACCCA AAAAGTTACC AGGCGATGAT
    AAACTAATGA AAAACCGTGC AGATCATCGA TCCAGTCCTA ATGTGGCCAA TCAACCCCAG
    AGTCCAGGTG GAAAAACTAC TTATTCAAGC AGCTCAACAG TGAAGATAAC ATCTGTCTCC
    ACTGGAAACC TATGCACTGA GGAACAGACT GCACCAGCAA GACCAGAAGC TTATCCTATC
    CCTACACAAA CATATGCAAG AGAGTATTTT ACTTTTCCAA CTCCAAAATC TCAAGATAGA
    ATAGGACCAG CTCAGAACCA ATGGCAAGGC TATGAGGATA ATTTCCAAGT TCAGATGGGT
    AACAACCATG CCAGAAATAC TTCGGCTCAA CAAGTTGCTT ATTCTGAGGA AGAGCTAAGG
    GATAATTTAA CATTCCAGTC AGAAAGAGGG CGCGTTGAAC CCCAAGTGCG GAAAGATGTG
    GAACATCTGG ATGGTAATGA TACTTGGTTC AATCGAGGCT CGTCTATGGA ATCAAGTGCA
    TCTTGTCAAG ACCTTAATAA TTCAAAGACA CATTCTGTAG GTTCTTTGAG CAAAACAGGA
    CCTGGTCGCT GGAAAACACC AATTTCTTCT CAGTCAGCTA CTGTTCTTTT GCCGCAGCCT
    GTTAGAACAG ACCTTCCTCC TCCACCACCT CCACCAGTTC ATTATGGAGC AGAATTTGAG
    GGAGTCCCAA TAGATTTACC TCTACCACCC CCACCATCTG AGAGCCAACT AGGGCTTTCA
    ACAAACTCAC AGGCAGCTGC ACTAGAAAGA AAGAAGCGGG AAGAACATCA GAGGTGGTAT
    GAGAAAGAGA AGGCACGTTT GGAAGAAGAA AGAGAAAGGA AAAGGCGAGA GCAGGAAAGA
    AAGCTAGGCC AGGTTCGTCC TCAGCCACCA ACTATACCTC CACAACCAGT AAATCCACAA
    TCTCCTCCGT TGATTCCTCC TCCAAATGTT GCACAAGCCA GAACAGAGAA ACCTGCCACT
    CTTCCTCGGC AACAAGAAAC TGTTATCAGG GAGCTACAAC CTCAGCAGCA GCCACGAACC
    ATAGAAAGAA GAGACCTCCA GTACATTACA GTCAGTAAAG AGGAGCTTTC TTCCAGTGAC
    AGCCTTTCCC CAGATCCCTG GAAAAGGGAT GCCAAGGAGA AGCTAGAAAA ACAGCAGCAG
    ATGCATATAG TGGATATGCT GAGCAAAGAG ATCAATGAAC TGCAAAACAA GCCTGACCGA
    ACTTCTGAAG AAAATGACCG ACTCCGCAAA TTAATGCTTG AATGGCAGTT TCAGAAGAGA
    CTACAAGAGT CCCAGCAGAA GGATGACGAT GACGACGATG ACGATGATGA TGATGATGAT
    GTGGATACCA TGCTCATCAT GCAGCGTCTG GAAGCTGAAA GGAAAGCAAG GCAGACCGCT
    ATGCCAGCAA TCTCTATTCT AGATTTGTTA CAGGATGAAG AACGGAGACG GCAACAACAT
    TTGGAAGAGG CGCGCAAACG GGAGGCAGAA GATCGCCTTA GGCAGGAAGA AGAGCATCGT
    CATCAAGAGG AGGAGCGGGC AAGGAGAGAG GCTGAAGAAA AGAGGAGACA GGAAGAAGAG
    TACTACAACC GCTTGGAAGC TGAGAGGCGC AGAATTCATG AAGAAGCAGA GCGGCGATTG
    TTAGACCCTG AAGAGCCTGG TCTGTACAGA CCTCCACTCC CACGGGAATA TGAGCTTTCC
    TCCCCAACCC TCCCACCTAA TCCTCCGCCT CCCCCTCCTC AGCGTAACAC CTCTTACCTC
    AAAACACAAG CCCTCTCCCC TGACACAGTT TACACTGCCA AGTTTGTTAC ATACACTGAT
    GATGAGGAAG ACACAAACCT ATCAGGTCAG GATAAGTACT CCAGCATGAG AAAATCTTAT
    GGTGACCTCC CTCCAGCTGC TCATAAACCA CAGCTGCCTG CTGCACGGCT GCCTCGTCCT
    GTATCCGATG GCATCTTTCT TTCCGGCTCA TTTCAGCCCC CTTCAGCCAA TGCCAACAGT
    ACTGCTTCCA AAACGGGTCC ACCTATAGCA AATAAACCAA GTTTCCTCCA TTACAGCAGC
    TCCAAAGGAC CAAATTCTTA TTCTGGATCA TCTGGTGCAG CAGTTCCTGG TAATGAAGTT
    TATAAAGATC CGAGGGATGT GCGACTAAAA AGCCAAGATG GAGAAGCTTA TAGCACTGGT
    GGAGCCCCAG AGAGCCTAAC CTTTAAGGAG CGCCAGCGGC TCTTCTCTCA GGGCCAGGAT
    GTGTCTAACA AAGTGAAAGC TTCCAGAAAA TTAATGGAAC TGGAAAATGA GTTAAACACA
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758153.1
    CDS1000..6705
    Translation

    Target ORF information:

    RefSeq Version XM_031902293.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT
    CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA
    AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG
    TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA
    GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG
    GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT
    ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT
    GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA
    CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT
    TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA
    TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA
    GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG
    TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG
    GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT
    TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT
    TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG
    TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA
    GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT
    ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT
    GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA
    AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT
    GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA
    GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA
    CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT
    ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC
    AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT
    GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT
    GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGGAAAT AGACAAAGGT
    AACCAGAAGC AAAAGAATAT TGCTGGAGCT CTTGCTTTCT GGATGGCAAA CGCTTCAGAG
    CTTCTCAATT TCATAAAGCA AGACCGAGAT CTCAATCGTA TCACATTGGA TGCACAAGAT
    GTTCTTGCAC ATTTAGTTCA GATGGCATTC AAATACCTGG TTCACTGTCT TCAATCTGAA
    TTGACCAGCT CTATGCCTGC TTTTATAGAT GATCCAGAAG AAAATGATTC CCAAAGGCCT
    AAAATAGAAG ACGTTTTGCA TACTCTGACA GGTGCCATGT CACTTCTAAG ACGTTGCCGG
    GTCAACGCAG CACTAACTAT TCAGCTGTTC TCGCAGCTAT TCCATTTCAT CAACATGTGG
    CTGTTCAACA GGCTTGTAAC AGATCCAGAC TCTGGTTTAT GCTCTCACTA TTGGGGGGCA
    ATAATTCGGC AGCAGCTTGC ACACATTGAG GCATGGGCTG AAAAACAAGG CCTCGAATTA
    GCTGCAGATT GTCATCTTAG CAGAATAGTT CAGGCTACTA CTCTTCTCAC TATGGACAAG
    TACTCGCATC AGGATATTCC AAATATTAAT AATACATGTT TTAAGCTGAA TTCCTTACAA
    CTGCAAGCCC TATTGCAAAA TTACCACTGT GCTCCTGATG AACCGTACAT CCCAACGGAG
    TTGATAGACA GTGTGGTGTC TGTAGCTGAA AACACTGCAG ATGAATTGGC TCGAAGTGAT
    GGCAGAGAAG TTCAGCTTGA GGAGGATCCT GATCTGCAAC TCCCTTTCCT TTTGCCTGAA
    GATGGTTATT CATGTGATGT GGTTAGAAAT ATTCCTAATG GCCTTCAAGA GTTTTTAGAA
    CCGCTCTGCC AGAGAGGTTT TTGCAGACTT GTCCCTCACT CACGGTCACC AGGCATGTGG
    ACAATTTACT TTGAAGGAGC TGAATTTGAA AATAATTTGT TGACCGACAA TACAGACCTG
    CCATTAAGAA AAGAACCTGA AATAATCACT GTGACACTTA AAAAGCAGAA TGGAATGGGT
    TTGAGTATTG TTGCAGCAAA GGGTGCTGGA CAAGAAAAAC TTGGGATTTA CATTAAGTCT
    GTGGTGAAAG GCGGTGCTGC AGAAGTGGAT GGTCGTTTAG CTGCAGGGGA CCAACTTATA
    AGTGTGGATG GGCAAAGTTT AGTTGGTTTG TCCCAAGAAA GAGCTGCAGA GCTTATGACT
    AGAACTGGGG CCGTAGTAAC CCTTGAGGTC GCAAAGCAAG GCGCAATATA CCATGGACTT
    GCCACACTAC TAAACCAACC ATCTCCTCTC ATGCAAAGAG ATCGACGTGG CTCAGGAAAA
    CCACGACCAA AGAGTGAAGG TTTTGAGCTT TACAATAATG CTTCCCAAAA TGGGTCTCCT
    GATAGCCCCC AGACACAATG GCCAGAATAC ACCGAACCCA AAAAGTTACC AGGCGATGAT
    AAACTAATGA AAAACCGTGC AGATCATCGA TCCAGTCCTA ATGTGGCCAA TCAACCCCAG
    AGTCCAGGTG GAAAAACTAC TTATTCAAGC AGCTCAACAG TGAAGATAAC ATCTGTCTCC
    ACTGGAAACC TATGCACTGA GGAACAGACT GCACCAGCAA GACCAGAAGC TTATCCTATC
    CCTACACAAA CATATGCAAG AGAGTATTTT ACTTTTCCAA CTCCAAAATC TCAAGATAGA
    ATAGGACCAG CTCAGAACCA ATGGCAAGGC TATGAGGATA ATTTCCAAGT TCAGATGGGT
    AACAACCATG CCAGAAATAC TTCGGCTCAA CAAGTTGCTT ATTCTGAGGA AGAGCTAAGG
    GATAATTTAA CATTCCAGTC AGAAAGAGGG CGCGTTGAAC CCCAAGTGCG GAAAGATGTG
    GAACATCTGG ATGGTAATGA TACTTGGTTC AATCGAGGCT CGTCTATGGA ATCAAGTGCA
    TCTTGTCAAG ACCTTAATAA TTCAAAGACA CATTCTGTAG GTTCTTTGAG CAAAACAGGA
    CCTGGTCGCT GGAAAACACC AATTTCTTCT CAGTCAGCTA CTGTTCTTTT GCCGCAGCCT
    GTTAGAACAG ACCTTCCTCC TCCACCACCT CCACCAGTTC ATTATGGAGC AGAATTTGAG
    GGAGTCCCAA TAGATTTACC TCTACCACCC CCACCATCTG AGAGCCAACT AGGGCTTTCA
    ACAAACTCAC AGGCAGCTGC ACTAGAAAGA AAGAAGCGGG AAGAACATCA GAGGTGGTAT
    GAGAAAGAGA AGGCACGTTT GGAAGAAGAA AGAGAAAGGA AAAGGCGAGA GCAGGAAAGA
    AAGCTAGGCC AGGTTCGTCC TCAGCCACCA ACTATACCTC CACAACCAGT AAATCCACAA
    TCTCCTCCGT TGATTCCTCC TCCAAATGTT GCACAAGCCA GAACAGAGAA ACCTGCCACT
    CTTCCTCGGC AACAAGAAAC TGTTATCAGG GAGCTACAAC CTCAGCAGCA GCCACGAACC
    ATAGAAAGAA GAGACCTCCA GTACATTACA GTCAGTAAAG AGGAGCTTTC TTCCAGTGAC
    AGCCTTTCCC CAGATCCCTG GAAAAGGGAT GCCAAGGAGA AGCTAGAAAA ACAGCAGCAG
    ATGCATATAG TGGATATGCT GAGCAAAGAG ATCAATGAAC TGCAAAACAA GCCTGACCGA
    ACTTCTGAAG AAAATGACCG ACTCCGCAAA TTAATGCTTG AATGGCAGTT TCAGAAGAGA
    CTACAAGAGT CCCAGCAGAA GGATGACGAT GACGACGATG ACGATGATGA TGATGATGAT
    GTGGATACCA TGCTCATCAT GCAGCGTCTG GAAGCTGAAA GGAAAGCAAG GCAGACCGCT
    ATGCCAGCAA TCTCTATTCT AGATTTGTTA CAGGATGAAG AACGGAGACG GCAACAACAT
    TTGGAAGAGG CGCGCAAACG GGAGGCAGAA GATCGCCTTA GGCAGGAAGA AGAGCATCGT
    CATCAAGAGG AGGAGCGGGC AAGGAGAGAG GCTGAAGAAA AGAGGAGACA GGAAGAAGAG
    TACTACAACC GCTTGGAAGC TGAGAGGCGC AGAATTCATG AAGAAGCAGA GCGGCGATTG
    TTAGACCCTG AAGAGCCTGG TCTGTACAGA CCTCCACTCC CACGGGAATA TGAGCTTTCC
    TCCCCAACCC TCCCACCTAA TCCTCCGCCT CCCCCTCCTC AGCGTAACAC CTCTTACCTC
    AAAACACAAG CCCTCTCCCC TGACACAGTT TACACTGCCA AGTTTGTTAC ATACACTGAT
    GATGAGGAAG ACACAAACCT ATCAGGTCAG GATAAGTACT CCAGCATGAG AAAATCTTAT
    GGTGACCTCC CTCCAGCTGC TCATAAACCA CAGCTGCCTG CTGCACGGCT GCCTCGTCCT
    GTATCCGATG GCATCTTTCT TTCCGGCTCA TTTCAGCCCC CTTCAGCCAA TGCCAACAGT
    ACTGCTTCCA AAACGGGTCC ACCTATAGCA AATAAACCAA GTTTCCTCCA TTACAGCAGC
    TCCAAAGGAC CAAATTCTTA TTCTGGATCA TCTGGTGCAG CAGTTCCTGG TAATGAAGTT
    TATAAAGATC CGAGGGATGT GCGACTAAAA AGCCAAGATG GAGAAGCTTA TAGCACTGGT
    GGAGCCCCAG AGAGCCTAAC CTTTAAGGAG CGCCAGCGGC TCTTCTCTCA GGGCCAGGAT
    GTGTCTAACA AAGTGAAAGC TTCCAGAAAA TTAATGGAAC TGGAAAATGA GTTAAACACA
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58613
    Clone ID Related Accession (Same CDS sequence) XM_031902294.1
    Accession Version XM_031902294.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT
    ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG
    CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT
    CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT
    GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG
    CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT
    ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA
    ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT
    GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT
    AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT
    CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA
    AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG
    TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT
    GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT
    GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT
    TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA
    GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT
    GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA
    AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT
    GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT
    TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA
    ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA
    CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA
    GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA
    TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT
    GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT
    ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT
    GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA
    AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC
    CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT
    ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG
    TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT
    GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT
    AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA
    CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT
    CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA
    CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT
    GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT
    TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT
    CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT
    CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA
    ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC
    CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC
    TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG
    CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC
    CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG
    AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC
    ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGCAGACCG CTATGCCAGC AATCTCTATT
    CTAGATTTGT TACAGGATGA AGAACGGAGA CGGCAACAAC ATTTGGAAGA GGCGCGCAAA
    CGGGAGGCAG AAGATCGCCT TAGGCAGGAA GAAGAGCATC GTCATCAAGA GGAGGAGCGG
    GCAAGGAGAG AGGCTGAAGA AAAGAGGAGA CAGGAAGAAG AGTACTACAA CCGCTTGGAA
    GCTGAGAGGC GCAGAATTCA TGAAGAAGCA GAGCGGCGAT TGTTAGACCC TGAAGAGCCT
    GGTCTGTACA GACCTCCACT CCCACGGGAA TATGAGCTTT CCTCCCCAAC CCTCCCACCT
    AATCCTCCGC CTCCCCCTCC TCAGCGTAAC ACCTCTTACC TCAAAACACA AGCCCTCTCC
    CCTGACACAG TTTACACTGC CAAGTTTGTT ACATACACTG ATGATGAGGA AGACACAAAC
    CTATCAGGTC AGGATAAGTA CTCCAGCATG AGAAAATCTT ATGGTGACCT CCCTCCAGCT
    GCTCATAAAC CACAGCTGCC TGCTGCACGG CTGCCTCGTC CTGTATCCGA TGGCATCTTT
    CTTTCCGGCT CATTTCAGCC CCCTTCAGCC AATGCCAACA GTACTGCTTC CAAAACGGGT
    CCACCTATAG CAAATAAACC AAGTTTCCTC CATTACAGCA GCTCCAAAGG ACCAAATTCT
    TATTCTGGAT CATCTGGTGC AGCAGTTCCT GGTAATGAAG TTTATAAAGA TCCGAGGGAT
    GTGCGACTAA AAAGCCAAGA TGGAGAAGCT TATAGCACTG GTGGAGCCCC AGAGAGCCTA
    ACCTTTAAGG AGCGCCAGCG GCTCTTCTCT CAGGGCCAGG ATGTGTCTAA CAAAGTGAAA
    GCTTCCAGAA AATTAATGGA ACTGGAAAAT GAGTTAAACA CAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758154.1
    CDS998..6685
    Translation

    Target ORF information:

    RefSeq Version XM_031902294.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902294.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT
    ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG
    CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT
    CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT
    GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG
    CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT
    ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA
    ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT
    GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT
    AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT
    CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA
    AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG
    TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT
    GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT
    GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT
    TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA
    GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT
    GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA
    AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT
    GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT
    TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA
    ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA
    CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA
    GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA
    TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT
    GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT
    ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT
    GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA
    AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC
    CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT
    ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG
    TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT
    GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT
    AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA
    CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT
    CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA
    CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT
    GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT
    TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT
    CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT
    CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA
    ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC
    CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC
    TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG
    CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC
    CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG
    AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC
    ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGCAGACCG CTATGCCAGC AATCTCTATT
    CTAGATTTGT TACAGGATGA AGAACGGAGA CGGCAACAAC ATTTGGAAGA GGCGCGCAAA
    CGGGAGGCAG AAGATCGCCT TAGGCAGGAA GAAGAGCATC GTCATCAAGA GGAGGAGCGG
    GCAAGGAGAG AGGCTGAAGA AAAGAGGAGA CAGGAAGAAG AGTACTACAA CCGCTTGGAA
    GCTGAGAGGC GCAGAATTCA TGAAGAAGCA GAGCGGCGAT TGTTAGACCC TGAAGAGCCT
    GGTCTGTACA GACCTCCACT CCCACGGGAA TATGAGCTTT CCTCCCCAAC CCTCCCACCT
    AATCCTCCGC CTCCCCCTCC TCAGCGTAAC ACCTCTTACC TCAAAACACA AGCCCTCTCC
    CCTGACACAG TTTACACTGC CAAGTTTGTT ACATACACTG ATGATGAGGA AGACACAAAC
    CTATCAGGTC AGGATAAGTA CTCCAGCATG AGAAAATCTT ATGGTGACCT CCCTCCAGCT
    GCTCATAAAC CACAGCTGCC TGCTGCACGG CTGCCTCGTC CTGTATCCGA TGGCATCTTT
    CTTTCCGGCT CATTTCAGCC CCCTTCAGCC AATGCCAACA GTACTGCTTC CAAAACGGGT
    CCACCTATAG CAAATAAACC AAGTTTCCTC CATTACAGCA GCTCCAAAGG ACCAAATTCT
    TATTCTGGAT CATCTGGTGC AGCAGTTCCT GGTAATGAAG TTTATAAAGA TCCGAGGGAT
    GTGCGACTAA AAAGCCAAGA TGGAGAAGCT TATAGCACTG GTGGAGCCCC AGAGAGCCTA
    ACCTTTAAGG AGCGCCAGCG GCTCTTCTCT CAGGGCCAGG ATGTGTCTAA CAAAGTGAAA
    GCTTCCAGAA AATTAATGGA ACTGGAAAAT GAGTTAAACA CAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58614
    Clone ID Related Accession (Same CDS sequence) XM_031902295.1
    Accession Version XM_031902295.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGTTACAG
    GATGAAGAAC GGAGACGGCA ACAACATTTG GAAGAGGCGC GCAAACGGGA GGCAGAAGAT
    CGCCTTAGGC AGGAAGAAGA GCATCGTCAT CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT
    GAAGAAAAGA GGAGACAGGA AGAAGAGTAC TACAACCGCT TGGAAGCTGA GAGGCGCAGA
    ATTCATGAAG AAGCAGAGCG GCGATTGTTA GACCCTGAAG AGCCTGGTCT GTACAGACCT
    CCACTCCCAC GGGAATATGA GCTTTCCTCC CCAACCCTCC CACCTAATCC TCCGCCTCCC
    CCTCCTCAGC GTAACACCTC TTACCTCAAA ACACAAGCCC TCTCCCCTGA CACAGTTTAC
    ACTGCCAAGT TTGTTACATA CACTGATGAT GAGGAAGACA CAAACCTATC AGGTCAGGAT
    AAGTACTCCA GCATGAGAAA ATCTTATGGT GACCTCCCTC CAGCTGCTCA TAAACCACAG
    CTGCCTGCTG CACGGCTGCC TCGTCCTGTA TCCGATGGCA TCTTTCTTTC CGGCTCATTT
    CAGCCCCCTT CAGCCAATGC CAACAGTACT GCTTCCAAAA CGGGTCCACC TATAGCAAAT
    AAACCAAGTT TCCTCCATTA CAGCAGCTCC AAAGGACCAA ATTCTTATTC TGGATCATCT
    GGTGCAGCAG TTCCTGGTAA TGAAGTTTAT AAAGATCCGA GGGATGTGCG ACTAAAAAGC
    CAAGATGGAG AAGCTTATAG CACTGGTGGA GCCCCAGAGA GCCTAACCTT TAAGGAGCGC
    CAGCGGCTCT TCTCTCAGGG CCAGGATGTG TCTAACAAAG TGAAAGCTTC CAGAAAATTA
    ATGGAACTGG AAAATGAGTT AAACACAAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758155.1
    CDS997..6669
    Translation

    Target ORF information:

    RefSeq Version XM_031902295.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902295.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGTTACAG
    GATGAAGAAC GGAGACGGCA ACAACATTTG GAAGAGGCGC GCAAACGGGA GGCAGAAGAT
    CGCCTTAGGC AGGAAGAAGA GCATCGTCAT CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT
    GAAGAAAAGA GGAGACAGGA AGAAGAGTAC TACAACCGCT TGGAAGCTGA GAGGCGCAGA
    ATTCATGAAG AAGCAGAGCG GCGATTGTTA GACCCTGAAG AGCCTGGTCT GTACAGACCT
    CCACTCCCAC GGGAATATGA GCTTTCCTCC CCAACCCTCC CACCTAATCC TCCGCCTCCC
    CCTCCTCAGC GTAACACCTC TTACCTCAAA ACACAAGCCC TCTCCCCTGA CACAGTTTAC
    ACTGCCAAGT TTGTTACATA CACTGATGAT GAGGAAGACA CAAACCTATC AGGTCAGGAT
    AAGTACTCCA GCATGAGAAA ATCTTATGGT GACCTCCCTC CAGCTGCTCA TAAACCACAG
    CTGCCTGCTG CACGGCTGCC TCGTCCTGTA TCCGATGGCA TCTTTCTTTC CGGCTCATTT
    CAGCCCCCTT CAGCCAATGC CAACAGTACT GCTTCCAAAA CGGGTCCACC TATAGCAAAT
    AAACCAAGTT TCCTCCATTA CAGCAGCTCC AAAGGACCAA ATTCTTATTC TGGATCATCT
    GGTGCAGCAG TTCCTGGTAA TGAAGTTTAT AAAGATCCGA GGGATGTGCG ACTAAAAAGC
    CAAGATGGAG AAGCTTATAG CACTGGTGGA GCCCCAGAGA GCCTAACCTT TAAGGAGCGC
    CAGCGGCTCT TCTCTCAGGG CCAGGATGTG TCTAACAAAG TGAAAGCTTC CAGAAAATTA
    ATGGAACTGG AAAATGAGTT AAACACAAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58615
    Clone ID Related Accession (Same CDS sequence) XM_031902296.1
    Accession Version XM_031902296.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGGATGAA
    GAACGGAGAC GGCAACAACA TTTGGAAGAG GCGCGCAAAC GGGAGGCAGA AGATCGCCTT
    AGGCAGGAAG AAGAGCATCG TCATCAAGAG GAGGAGCGGG CAAGGAGAGA GGCTGAAGAA
    AAGAGGAGAC AGGAAGAAGA GTACTACAAC CGCTTGGAAG CTGAGAGGCG CAGAATTCAT
    GAAGAAGCAG AGCGGCGATT GTTAGACCCT GAAGAGCCTG GTCTGTACAG ACCTCCACTC
    CCACGGGAAT ATGAGCTTTC CTCCCCAACC CTCCCACCTA ATCCTCCGCC TCCCCCTCCT
    CAGCGTAACA CCTCTTACCT CAAAACACAA GCCCTCTCCC CTGACACAGT TTACACTGCC
    AAGTTTGTTA CATACACTGA TGATGAGGAA GACACAAACC TATCAGGTCA GGATAAGTAC
    TCCAGCATGA GAAAATCTTA TGGTGACCTC CCTCCAGCTG CTCATAAACC ACAGCTGCCT
    GCTGCACGGC TGCCTCGTCC TGTATCCGAT GGCATCTTTC TTTCCGGCTC ATTTCAGCCC
    CCTTCAGCCA ATGCCAACAG TACTGCTTCC AAAACGGGTC CACCTATAGC AAATAAACCA
    AGTTTCCTCC ATTACAGCAG CTCCAAAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA
    GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT
    GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG
    CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA
    CTGGAAAATG AGTTAAACAC AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758156.1
    CDS998..6664
    Translation

    Target ORF information:

    RefSeq Version XM_031902296.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902296.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGGATGAA
    GAACGGAGAC GGCAACAACA TTTGGAAGAG GCGCGCAAAC GGGAGGCAGA AGATCGCCTT
    AGGCAGGAAG AAGAGCATCG TCATCAAGAG GAGGAGCGGG CAAGGAGAGA GGCTGAAGAA
    AAGAGGAGAC AGGAAGAAGA GTACTACAAC CGCTTGGAAG CTGAGAGGCG CAGAATTCAT
    GAAGAAGCAG AGCGGCGATT GTTAGACCCT GAAGAGCCTG GTCTGTACAG ACCTCCACTC
    CCACGGGAAT ATGAGCTTTC CTCCCCAACC CTCCCACCTA ATCCTCCGCC TCCCCCTCCT
    CAGCGTAACA CCTCTTACCT CAAAACACAA GCCCTCTCCC CTGACACAGT TTACACTGCC
    AAGTTTGTTA CATACACTGA TGATGAGGAA GACACAAACC TATCAGGTCA GGATAAGTAC
    TCCAGCATGA GAAAATCTTA TGGTGACCTC CCTCCAGCTG CTCATAAACC ACAGCTGCCT
    GCTGCACGGC TGCCTCGTCC TGTATCCGAT GGCATCTTTC TTTCCGGCTC ATTTCAGCCC
    CCTTCAGCCA ATGCCAACAG TACTGCTTCC AAAACGGGTC CACCTATAGC AAATAAACCA
    AGTTTCCTCC ATTACAGCAG CTCCAAAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA
    GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT
    GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG
    CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA
    CTGGAAAATG AGTTAAACAC AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58616
    Clone ID Related Accession (Same CDS sequence) XM_031902297.1
    Accession Version XM_031902297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGCAGACC
    GCTATGCCAG CAATCTCTAT TCTAGATTTG TTACAGGATG AAGAACGGAG ACGGCAACAA
    CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT
    CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA
    GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA
    TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT
    TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC
    CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT
    GATGATGAGG AAGACACAAA CCTATCAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA
    GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT
    GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG
    CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA
    CTGGAAAATG AGTTAAACAC AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758157.1
    CDS1000..6486
    Translation

    Target ORF information:

    RefSeq Version XM_031902297.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGCAGACC
    GCTATGCCAG CAATCTCTAT TCTAGATTTG TTACAGGATG AAGAACGGAG ACGGCAACAA
    CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT
    CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA
    GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA
    TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT
    TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC
    CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT
    GATGATGAGG AAGACACAAA CCTATCAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA
    GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT
    GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG
    CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA
    CTGGAAAATG AGTTAAACAC AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58617
    Clone ID Related Accession (Same CDS sequence) XM_031902298.1
    Accession Version XM_031902298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGTTACAG
    GATGAAGAAC GGAGACGGCA ACAACATTTG GAAGAGGCGC GCAAACGGGA GGCAGAAGAT
    CGCCTTAGGC AGGAAGAAGA GCATCGTCAT CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT
    GAAGAAAAGA GGAGACAGGA AGAAGAGTAC TACAACCGCT TGGAAGCTGA GAGGCGCAGA
    ATTCATGAAG AAGCAGAGCG GCGATTGTTA GACCCTGAAG AGCCTGGTCT GTACAGACCT
    CCACTCCCAC GGGAATATGA GCTTTCCTCC CCAACCCTCC CACCTAATCC TCCGCCTCCC
    CCTCCTCAGC GTAACACCTC TTACCTCAAA ACACAAGCCC TCTCCCCTGA CACAGTTTAC
    ACTGCCAAGT TTGTTACATA CACTGATGAT GAGGAAGACA CAAACCTATC AGGACCAAAT
    TCTTATTCTG GATCATCTGG TGCAGCAGTT CCTGGTAATG AAGTTTATAA AGATCCGAGG
    GATGTGCGAC TAAAAAGCCA AGATGGAGAA GCTTATAGCA CTGGTGGAGC CCCAGAGAGC
    CTAACCTTTA AGGAGCGCCA GCGGCTCTTC TCTCAGGGCC AGGATGTGTC TAACAAAGTG
    AAAGCTTCCA GAAAATTAAT GGAACTGGAA AATGAGTTAA ACACAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758158.1
    CDS998..6448
    Translation

    Target ORF information:

    RefSeq Version XM_031902298.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGTTACAG
    GATGAAGAAC GGAGACGGCA ACAACATTTG GAAGAGGCGC GCAAACGGGA GGCAGAAGAT
    CGCCTTAGGC AGGAAGAAGA GCATCGTCAT CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT
    GAAGAAAAGA GGAGACAGGA AGAAGAGTAC TACAACCGCT TGGAAGCTGA GAGGCGCAGA
    ATTCATGAAG AAGCAGAGCG GCGATTGTTA GACCCTGAAG AGCCTGGTCT GTACAGACCT
    CCACTCCCAC GGGAATATGA GCTTTCCTCC CCAACCCTCC CACCTAATCC TCCGCCTCCC
    CCTCCTCAGC GTAACACCTC TTACCTCAAA ACACAAGCCC TCTCCCCTGA CACAGTTTAC
    ACTGCCAAGT TTGTTACATA CACTGATGAT GAGGAAGACA CAAACCTATC AGGACCAAAT
    TCTTATTCTG GATCATCTGG TGCAGCAGTT CCTGGTAATG AAGTTTATAA AGATCCGAGG
    GATGTGCGAC TAAAAAGCCA AGATGGAGAA GCTTATAGCA CTGGTGGAGC CCCAGAGAGC
    CTAACCTTTA AGGAGCGCCA GCGGCTCTTC TCTCAGGGCC AGGATGTGTC TAACAAAGTG
    AAAGCTTCCA GAAAATTAAT GGAACTGGAA AATGAGTTAA ACACAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58618
    Clone ID Related Accession (Same CDS sequence) XM_031902299.1
    Accession Version XM_031902299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGGATGAA
    GAACGGAGAC GGCAACAACA TTTGGAAGAG GCGCGCAAAC GGGAGGCAGA AGATCGCCTT
    AGGCAGGAAG AAGAGCATCG TCATCAAGAG GAGGAGCGGG CAAGGAGAGA GGCTGAAGAA
    AAGAGGAGAC AGGAAGAAGA GTACTACAAC CGCTTGGAAG CTGAGAGGCG CAGAATTCAT
    GAAGAAGCAG AGCGGCGATT GTTAGACCCT GAAGAGCCTG GTCTGTACAG ACCTCCACTC
    CCACGGGAAT ATGAGCTTTC CTCCCCAACC CTCCCACCTA ATCCTCCGCC TCCCCCTCCT
    CAGCGTAACA CCTCTTACCT CAAAACACAA GCCCTCTCCC CTGACACAGT TTACACTGCC
    AAGTTTGTTA CATACACTGA TGATGAGGAA GACACAAACC TATCAGGACC AAATTCTTAT
    TCTGGATCAT CTGGTGCAGC AGTTCCTGGT AATGAAGTTT ATAAAGATCC GAGGGATGTG
    CGACTAAAAA GCCAAGATGG AGAAGCTTAT AGCACTGGTG GAGCCCCAGA GAGCCTAACC
    TTTAAGGAGC GCCAGCGGCT CTTCTCTCAG GGCCAGGATG TGTCTAACAA AGTGAAAGCT
    TCCAGAAAAT TAATGGAACT GGAAAATGAG TTAAACACAA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758159.1
    CDS999..6443
    Translation

    Target ORF information:

    RefSeq Version XM_031902299.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA
    GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA
    GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA
    GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT
    GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG
    CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC
    CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG
    TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG
    GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA
    TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC
    AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA
    CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG
    GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT
    GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT
    GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA
    GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG
    TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC
    CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG
    GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG
    TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT
    ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG
    ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA
    CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA
    AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT
    CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT
    GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC
    CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC
    TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT
    ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT
    AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG
    GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA
    AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT
    GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT
    GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA
    GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG
    CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT
    GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT
    TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG
    TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA
    AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA
    CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC
    ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA
    ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT
    GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG
    CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC
    CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG
    AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT
    GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGGATGAA
    GAACGGAGAC GGCAACAACA TTTGGAAGAG GCGCGCAAAC GGGAGGCAGA AGATCGCCTT
    AGGCAGGAAG AAGAGCATCG TCATCAAGAG GAGGAGCGGG CAAGGAGAGA GGCTGAAGAA
    AAGAGGAGAC AGGAAGAAGA GTACTACAAC CGCTTGGAAG CTGAGAGGCG CAGAATTCAT
    GAAGAAGCAG AGCGGCGATT GTTAGACCCT GAAGAGCCTG GTCTGTACAG ACCTCCACTC
    CCACGGGAAT ATGAGCTTTC CTCCCCAACC CTCCCACCTA ATCCTCCGCC TCCCCCTCCT
    CAGCGTAACA CCTCTTACCT CAAAACACAA GCCCTCTCCC CTGACACAGT TTACACTGCC
    AAGTTTGTTA CATACACTGA TGATGAGGAA GACACAAACC TATCAGGACC AAATTCTTAT
    TCTGGATCAT CTGGTGCAGC AGTTCCTGGT AATGAAGTTT ATAAAGATCC GAGGGATGTG
    CGACTAAAAA GCCAAGATGG AGAAGCTTAT AGCACTGGTG GAGCCCCAGA GAGCCTAACC
    TTTAAGGAGC GCCAGCGGCT CTTCTCTCAG GGCCAGGATG TGTCTAACAA AGTGAAAGCT
    TCCAGAAAAT TAATGGAACT GGAAAATGAG TTAAACACAA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58619
    Clone ID Related Accession (Same CDS sequence) XM_031902300.1
    Accession Version XM_031902300.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT
    ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG
    CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT
    CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT
    GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG
    CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT
    ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA
    ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT
    GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT
    AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT
    CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA
    AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG
    TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT
    GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT
    GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT
    TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA
    GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT
    GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA
    AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT
    GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT
    TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA
    ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA
    CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA
    GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA
    TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT
    GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT
    ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT
    GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA
    AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC
    CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT
    ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG
    TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT
    GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT
    AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA
    CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT
    CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA
    CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT
    GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT
    TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT
    CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT
    CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA
    ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC
    CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC
    TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG
    CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC
    CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG
    AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC
    ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGTTACAGG ATGAAGAACG GAGACGGCAA
    CAACATTTGG AAGAGGCGCG CAAACGGGAG GCAGAAGATC GCCTTAGGCA GGAAGAAGAG
    CATCGTCATC AAGAGGAGGA GCGGGCAAGG AGAGAGGCTG AAGAAAAGAG GAGACAGGAA
    GAAGAGTACT ACAACCGCTT GGAAGCTGAG AGGCGCAGAA TTCATGAAGA AGCAGAGCGG
    CGATTGTTAG ACCCTGAAGA GCCTGGTCTG TACAGACCTC CACTCCCACG GGAATATGAG
    CTTTCCTCCC CAACCCTCCC ACCTAATCCT CCGCCTCCCC CTCCTCAGCG TAACACCTCT
    TACCTCAAAA CACAAGCCCT CTCCCCTGAC ACAGTTTACA CTGCCAAGTT TGTTACATAC
    ACTGATGATG AGGAAGACAC AAACCTATCA GGACCAAATT CTTATTCTGG ATCATCTGGT
    GCAGCAGTTC CTGGTAATGA AGTTTATAAA GATCCGAGGG ATGTGCGACT AAAAAGCCAA
    GATGGAGAAG CTTATAGCAC TGGTGGAGCC CCAGAGAGCC TAACCTTTAA GGAGCGCCAG
    CGGCTCTTCT CTCAGGGCCA GGATGTGTCT AACAAAGTGA AAGCTTCCAG AAAATTAATG
    GAACTGGAAA ATGAGTTAAA CACAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758160.1
    CDS997..6426
    Translation

    Target ORF information:

    RefSeq Version XM_031902300.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT
    ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG
    CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT
    CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT
    GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG
    CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT
    ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA
    ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT
    GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT
    AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT
    CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA
    AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG
    TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT
    GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT
    GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT
    TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA
    GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT
    GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA
    AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT
    GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT
    TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA
    ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA
    CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA
    GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA
    TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT
    GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT
    ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT
    GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA
    AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC
    CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT
    ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG
    TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT
    GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT
    AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA
    CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT
    CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA
    CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT
    GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT
    TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT
    CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT
    CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA
    ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC
    CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC
    TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG
    CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC
    CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG
    AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC
    ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGTTACAGG ATGAAGAACG GAGACGGCAA
    CAACATTTGG AAGAGGCGCG CAAACGGGAG GCAGAAGATC GCCTTAGGCA GGAAGAAGAG
    CATCGTCATC AAGAGGAGGA GCGGGCAAGG AGAGAGGCTG AAGAAAAGAG GAGACAGGAA
    GAAGAGTACT ACAACCGCTT GGAAGCTGAG AGGCGCAGAA TTCATGAAGA AGCAGAGCGG
    CGATTGTTAG ACCCTGAAGA GCCTGGTCTG TACAGACCTC CACTCCCACG GGAATATGAG
    CTTTCCTCCC CAACCCTCCC ACCTAATCCT CCGCCTCCCC CTCCTCAGCG TAACACCTCT
    TACCTCAAAA CACAAGCCCT CTCCCCTGAC ACAGTTTACA CTGCCAAGTT TGTTACATAC
    ACTGATGATG AGGAAGACAC AAACCTATCA GGACCAAATT CTTATTCTGG ATCATCTGGT
    GCAGCAGTTC CTGGTAATGA AGTTTATAAA GATCCGAGGG ATGTGCGACT AAAAAGCCAA
    GATGGAGAAG CTTATAGCAC TGGTGGAGCC CCAGAGAGCC TAACCTTTAA GGAGCGCCAG
    CGGCTCTTCT CTCAGGGCCA GGATGTGTCT AACAAAGTGA AAGCTTCCAG AAAATTAATG
    GAACTGGAAA ATGAGTTAAA CACAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58620
    Clone ID Related Accession (Same CDS sequence) XM_031902301.1
    Accession Version XM_031902301.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT
    CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA
    AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG
    TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA
    GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG
    GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT
    ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT
    GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA
    CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT
    TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA
    TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA
    GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG
    TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG
    GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT
    TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT
    TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG
    TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA
    GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT
    ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT
    GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA
    AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT
    GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA
    GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA
    CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT
    ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC
    AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT
    GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT
    GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGAAGCA AAAGAATATT
    GCTGGAGCTC TTGCTTTCTG GATGGCAAAC GCTTCAGAGC TTCTCAATTT CATAAAGCAA
    GACCGAGATC TCAATCGTAT CACATTGGAT GCACAAGATG TTCTTGCACA TTTAGTTCAG
    ATGGCATTCA AATACCTGGT TCACTGTCTT CAATCTGAAT TGACCAGCTC TATGCCTGCT
    TTTATAGATG ATCCAGAAGA AAATGATTCC CAAAGGCCTA AAATAGAAGA CGTTTTGCAT
    ACTCTGACAG GTGCCATGTC ACTTCTAAGA CGTTGCCGGG TCAACGCAGC ACTAACTATT
    CAGCTGTTCT CGCAGCTATT CCATTTCATC AACATGTGGC TGTTCAACAG GCTTGTAACA
    GATCCAGACT CTGGTTTATG CTCTCACTAT TGGGGGGCAA TAATTCGGCA GCAGCTTGCA
    CACATTGAGG CATGGGCTGA AAAACAAGGC CTCGAATTAG CTGCAGATTG TCATCTTAGC
    AGAATAGTTC AGGCTACTAC TCTTCTCACT ATGGACAAGT ACTCGCATCA GGATATTCCA
    AATATTAATA ATACATGTTT TAAGCTGAAT TCCTTACAAC TGCAAGCCCT ATTGCAAAAT
    TACCACTGTG CTCCTGATGA ACCGTACATC CCAACGGAGT TGATAGACAG TGTGGTGTCT
    GTAGCTGAAA ACACTGCAGA TGAATTGGCT CGAAGTGATG GCAGAGAAGT TCAGCTTGAG
    GAGGATCCTG ATCTGCAACT CCCTTTCCTT TTGCCTGAAG ATGGTTATTC ATGTGATGTG
    GTTAGAAATA TTCCTAATGG CCTTCAAGAG TTTTTAGAAC CGCTCTGCCA GAGAGGTTTT
    TGCAGACTTG TCCCTCACTC ACGGTCACCA GGCATGTGGA CAATTTACTT TGAAGGAGCT
    GAATTTGAAA ATAATTTGTT GACCGACAAT ACAGACCTGC CATTAAGAAA AGAACCTGAA
    ATAATCACTG TGACACTTAA AAAGCAGAAT GGAATGGGTT TGAGTATTGT TGCAGCAAAG
    GGTGCTGGAC AAGAAAAACT TGGGATTTAC ATTAAGTCTG TGGTGAAAGG CGGTGCTGCA
    GAAGTGGATG GTCGTTTAGC TGCAGGGGAC CAACTTATAA GTGTGGATGG GCAAAGTTTA
    GTTGGTTTGT CCCAAGAAAG AGCTGCAGAG CTTATGACTA GAACTGGGGC CGTAGTAACC
    CTTGAGGTCG CAAAGCAAGG CGCAATATAC CATGGACTTG CCACACTACT AAACCAACCA
    TCTCCTCTCA TGCAAAGAGA TCGACGTGGC TCAGGAAAAC CACGACCAAA GAGTGAAGGT
    TTTGAGCTTT ACAATAATGC TTCCCAAAAT GGGTCTCCTG ATAGCCCCCA GACACAATGG
    CCAGAATACA CCGAACCCAA AAAGTTACCA GGCGATGATA AACTAATGAA AAACCGTGCA
    GATCATCGAT CCAGTCCTAA TGTGGCCAAT CAACCCCAGA GTCCAGGTGG AAAAACTACT
    TATTCAAGCA GCTCAACAGT GAAGATAACA TCTGTCTCCA CTGGAAACCT ATGCACTGAG
    GAACAGACTG CACCAGCAAG ACCAGAAGCT TATCCTATCC CTACACAAAC ATATGCAAGA
    GAGTATTTTA CTTTTCCAAC TCCAAAATCT CAAGATAGAA TAGGACCAGC TCAGAACCAA
    TGGCAAGGCT ATGAGGATAA TTTCCAAGTT CAGATGGGTA ACAACCATGC CAGAAATACT
    TCGGCTCAAC AAGTTGCTTA TTCTGAGGAA GAGCTAAGGG ATAATTTAAC ATTCCAGTCA
    GAAAGAGGGC GCGTTGAACC CCAAGTGCGG AAAGATGTGG AACATCTGGA TGGTAATGAT
    ACTTGGTTCA ATCGAGGCTC GTCTATGGAA TCAAGTGCAT CTTGTCAAGA CCTTAATAAT
    TCAAAGACAC ATTCTGTAGG TTCTTTGAGC AAAACAGGAC CTGGTCGCTG GAAAACACCA
    ATTTCTTCTC AGTCAGCTAC TGTTCTTTTG CCGCAGCCTG TTAGAACAGA CCTTCCTCCT
    CCACCACCTC CACCAGTTCA TTATGGAGCA GAATTTGAGG GAGTCCCAAT AGATTTACCT
    CTACCACCCC CACCATCTGA GAGCCAACTA GGGCTTTCAA CAAACTCACA GGCAGCTGCA
    CTAGAAAGAA AGAAGCGGGA AGAACATCAG AGGTGGTATG AGAAAGAGAA GGCACGTTTG
    GAAGAAGAAA GAGAAAGGAA AAGGCGAGAG CAGGAAAGAA AGCTAGGCCA GGTTCGTCCT
    CAGCCACCAA CTATACCTCC ACAACCAGTA AATCCACAAT CTCCTCCGTT GATTCCTCCT
    CCAAATGTTG CACAAGCCAG AACAGAGAAA CCTGCCACTC TTCCTCGGCA ACAAGAAACT
    GTTATCAGGG AGCTACAACC TCAGCAGCAG CCACGAACCA TAGAAAGAAG AGACCTCCAG
    TACATTACAG TCAGTAAAGA GGAGCTTTCT TCCAGTGACA GCCTTTCCCC AGATCCCTGG
    AAAAGGGATG CCAAGGAGAA GCTAGAAAAA CAGCAGCAGA TGCATATAGT GGATATGCTG
    AGCAAAGAGA TCAATGAACT GCAAAACAAG CCTGACCGAA CTTCTGAAGA AAATGACCGA
    CTCCGCAAAT TAATGCTTGA ATGGCAGTTT CAGAAGAGAC TACAAGAGTC CCAGCAGAAG
    GATGACGATG ACGACGATGA CGATGATGAT GATGATGATG TGGATACCAT GCTCATCATG
    CAGCGTCTGG AAGCTGAAAG GAAAGCAAGG TTACAGGATG AAGAACGGAG ACGGCAACAA
    CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT
    CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA
    GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA
    TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT
    TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC
    CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT
    GATGATGAGG AAGACACAAA CCTATCAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA
    GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT
    GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG
    CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA
    CTGGAAAATG AGTTAAACAC AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758161.1
    CDS997..6423
    Translation

    Target ORF information:

    RefSeq Version XM_031902301.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902301.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT
    CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA
    AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG
    TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA
    GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG
    GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT
    ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT
    GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA
    CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT
    TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA
    TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA
    GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG
    TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG
    GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT
    TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT
    TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG
    TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA
    GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT
    ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT
    GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA
    AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT
    GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA
    GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA
    CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT
    ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC
    AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT
    GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT
    GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGAAGCA AAAGAATATT
    GCTGGAGCTC TTGCTTTCTG GATGGCAAAC GCTTCAGAGC TTCTCAATTT CATAAAGCAA
    GACCGAGATC TCAATCGTAT CACATTGGAT GCACAAGATG TTCTTGCACA TTTAGTTCAG
    ATGGCATTCA AATACCTGGT TCACTGTCTT CAATCTGAAT TGACCAGCTC TATGCCTGCT
    TTTATAGATG ATCCAGAAGA AAATGATTCC CAAAGGCCTA AAATAGAAGA CGTTTTGCAT
    ACTCTGACAG GTGCCATGTC ACTTCTAAGA CGTTGCCGGG TCAACGCAGC ACTAACTATT
    CAGCTGTTCT CGCAGCTATT CCATTTCATC AACATGTGGC TGTTCAACAG GCTTGTAACA
    GATCCAGACT CTGGTTTATG CTCTCACTAT TGGGGGGCAA TAATTCGGCA GCAGCTTGCA
    CACATTGAGG CATGGGCTGA AAAACAAGGC CTCGAATTAG CTGCAGATTG TCATCTTAGC
    AGAATAGTTC AGGCTACTAC TCTTCTCACT ATGGACAAGT ACTCGCATCA GGATATTCCA
    AATATTAATA ATACATGTTT TAAGCTGAAT TCCTTACAAC TGCAAGCCCT ATTGCAAAAT
    TACCACTGTG CTCCTGATGA ACCGTACATC CCAACGGAGT TGATAGACAG TGTGGTGTCT
    GTAGCTGAAA ACACTGCAGA TGAATTGGCT CGAAGTGATG GCAGAGAAGT TCAGCTTGAG
    GAGGATCCTG ATCTGCAACT CCCTTTCCTT TTGCCTGAAG ATGGTTATTC ATGTGATGTG
    GTTAGAAATA TTCCTAATGG CCTTCAAGAG TTTTTAGAAC CGCTCTGCCA GAGAGGTTTT
    TGCAGACTTG TCCCTCACTC ACGGTCACCA GGCATGTGGA CAATTTACTT TGAAGGAGCT
    GAATTTGAAA ATAATTTGTT GACCGACAAT ACAGACCTGC CATTAAGAAA AGAACCTGAA
    ATAATCACTG TGACACTTAA AAAGCAGAAT GGAATGGGTT TGAGTATTGT TGCAGCAAAG
    GGTGCTGGAC AAGAAAAACT TGGGATTTAC ATTAAGTCTG TGGTGAAAGG CGGTGCTGCA
    GAAGTGGATG GTCGTTTAGC TGCAGGGGAC CAACTTATAA GTGTGGATGG GCAAAGTTTA
    GTTGGTTTGT CCCAAGAAAG AGCTGCAGAG CTTATGACTA GAACTGGGGC CGTAGTAACC
    CTTGAGGTCG CAAAGCAAGG CGCAATATAC CATGGACTTG CCACACTACT AAACCAACCA
    TCTCCTCTCA TGCAAAGAGA TCGACGTGGC TCAGGAAAAC CACGACCAAA GAGTGAAGGT
    TTTGAGCTTT ACAATAATGC TTCCCAAAAT GGGTCTCCTG ATAGCCCCCA GACACAATGG
    CCAGAATACA CCGAACCCAA AAAGTTACCA GGCGATGATA AACTAATGAA AAACCGTGCA
    GATCATCGAT CCAGTCCTAA TGTGGCCAAT CAACCCCAGA GTCCAGGTGG AAAAACTACT
    TATTCAAGCA GCTCAACAGT GAAGATAACA TCTGTCTCCA CTGGAAACCT ATGCACTGAG
    GAACAGACTG CACCAGCAAG ACCAGAAGCT TATCCTATCC CTACACAAAC ATATGCAAGA
    GAGTATTTTA CTTTTCCAAC TCCAAAATCT CAAGATAGAA TAGGACCAGC TCAGAACCAA
    TGGCAAGGCT ATGAGGATAA TTTCCAAGTT CAGATGGGTA ACAACCATGC CAGAAATACT
    TCGGCTCAAC AAGTTGCTTA TTCTGAGGAA GAGCTAAGGG ATAATTTAAC ATTCCAGTCA
    GAAAGAGGGC GCGTTGAACC CCAAGTGCGG AAAGATGTGG AACATCTGGA TGGTAATGAT
    ACTTGGTTCA ATCGAGGCTC GTCTATGGAA TCAAGTGCAT CTTGTCAAGA CCTTAATAAT
    TCAAAGACAC ATTCTGTAGG TTCTTTGAGC AAAACAGGAC CTGGTCGCTG GAAAACACCA
    ATTTCTTCTC AGTCAGCTAC TGTTCTTTTG CCGCAGCCTG TTAGAACAGA CCTTCCTCCT
    CCACCACCTC CACCAGTTCA TTATGGAGCA GAATTTGAGG GAGTCCCAAT AGATTTACCT
    CTACCACCCC CACCATCTGA GAGCCAACTA GGGCTTTCAA CAAACTCACA GGCAGCTGCA
    CTAGAAAGAA AGAAGCGGGA AGAACATCAG AGGTGGTATG AGAAAGAGAA GGCACGTTTG
    GAAGAAGAAA GAGAAAGGAA AAGGCGAGAG CAGGAAAGAA AGCTAGGCCA GGTTCGTCCT
    CAGCCACCAA CTATACCTCC ACAACCAGTA AATCCACAAT CTCCTCCGTT GATTCCTCCT
    CCAAATGTTG CACAAGCCAG AACAGAGAAA CCTGCCACTC TTCCTCGGCA ACAAGAAACT
    GTTATCAGGG AGCTACAACC TCAGCAGCAG CCACGAACCA TAGAAAGAAG AGACCTCCAG
    TACATTACAG TCAGTAAAGA GGAGCTTTCT TCCAGTGACA GCCTTTCCCC AGATCCCTGG
    AAAAGGGATG CCAAGGAGAA GCTAGAAAAA CAGCAGCAGA TGCATATAGT GGATATGCTG
    AGCAAAGAGA TCAATGAACT GCAAAACAAG CCTGACCGAA CTTCTGAAGA AAATGACCGA
    CTCCGCAAAT TAATGCTTGA ATGGCAGTTT CAGAAGAGAC TACAAGAGTC CCAGCAGAAG
    GATGACGATG ACGACGATGA CGATGATGAT GATGATGATG TGGATACCAT GCTCATCATG
    CAGCGTCTGG AAGCTGAAAG GAAAGCAAGG TTACAGGATG AAGAACGGAG ACGGCAACAA
    CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT
    CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA
    GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA
    TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT
    TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC
    CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT
    GATGATGAGG AAGACACAAA CCTATCAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA
    GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT
    GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG
    CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA
    CTGGAAAATG AGTTAAACAC AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58621
    Clone ID Related Accession (Same CDS sequence) XM_031902302.1
    Accession Version XM_031902302.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT
    CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA
    AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG
    TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA
    GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG
    GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT
    ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT
    GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA
    CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT
    TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA
    TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA
    GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG
    TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG
    GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT
    TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT
    TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG
    TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA
    GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT
    ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT
    GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA
    AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT
    GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA
    GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA
    CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT
    ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC
    AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT
    GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT
    GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGAAGCA AAAGAATATT
    GCTGGAGCTC TTGCTTTCTG GATGGCAAAC GCTTCAGAGC TTCTCAATTT CATAAAGCAA
    GACCGAGATC TCAATCGTAT CACATTGGAT GCACAAGATG TTCTTGCACA TTTAGTTCAG
    ATGGCATTCA AATACCTGGT TCACTGTCTT CAATCTGAAT TGACCAGCTC TATGCCTGCT
    TTTATAGATG ATCCAGAAGA AAATGATTCC CAAAGGCCTA AAATAGAAGA CGTTTTGCAT
    ACTCTGACAG GTGCCATGTC ACTTCTAAGA CGTTGCCGGG TCAACGCAGC ACTAACTATT
    CAGCTGTTCT CGCAGCTATT CCATTTCATC AACATGTGGC TGTTCAACAG GCTTGTAACA
    GATCCAGACT CTGGTTTATG CTCTCACTAT TGGGGGGCAA TAATTCGGCA GCAGCTTGCA
    CACATTGAGG CATGGGCTGA AAAACAAGGC CTCGAATTAG CTGCAGATTG TCATCTTAGC
    AGAATAGTTC AGGCTACTAC TCTTCTCACT ATGGACAAGT ACTCGCATCA GGATATTCCA
    AATATTAATA ATACATGTTT TAAGCTGAAT TCCTTACAAC TGCAAGCCCT ATTGCAAAAT
    TACCACTGTG CTCCTGATGA ACCGTACATC CCAACGGAGT TGATAGACAG TGTGGTGTCT
    GTAGCTGAAA ACACTGCAGA TGAATTGGCT CGAAGTGATG GCAGAGAAGT TCAGCTTGAG
    GAGGATCCTG ATCTGCAACT CCCTTTCCTT TTGCCTGAAG ATGGTTATTC ATGTGATGTG
    GTTAGAAATA TTCCTAATGG CCTTCAAGAG TTTTTAGAAC CGCTCTGCCA GAGAGGTTTT
    TGCAGACTTG TCCCTCACTC ACGGTCACCA GGCATGTGGA CAATTTACTT TGAAGGAGCT
    GAATTTGAAA ATAATTTGTT GACCGACAAT ACAGACCTGC CATTAAGAAA AGAACCTGAA
    ATAATCACTG TGACACTTAA AAAGCAGAAT GGAATGGGTT TGAGTATTGT TGCAGCAAAG
    GGTGCTGGAC AAGAAAAACT TGGGATTTAC ATTAAGTCTG TGGTGAAAGG CGGTGCTGCA
    GAAGTGGATG GTCGTTTAGC TGCAGGGGAC CAACTTATAA GTGTGGATGG GCAAAGTTTA
    GTTGGTTTGT CCCAAGAAAG AGCTGCAGAG CTTATGACTA GAACTGGGGC CGTAGTAACC
    CTTGAGGTCG CAAAGCAAGG CGCAATATAC CATGGACTTG CCACACTACT AAACCAACCA
    TCTCCTCTCA TGCAAAGAGA TCGACGTGGC TCAGGAAAAC CACGACCAAA GAGTGAAGGT
    TTTGAGCTTT ACAATAATGC TTCCCAAAAT GGGTCTCCTG ATAGCCCCCA GACACAATGG
    CCAGAATACA CCGAACCCAA AAAGTTACCA GGCGATGATA AACTAATGAA AAACCGTGCA
    GATCATCGAT CCAGTCCTAA TGTGGCCAAT CAACCCCAGA GTCCAGGTGG AAAAACTACT
    TATTCAAGCA GCTCAACAGT GAAGATAACA TCTGTCTCCA CTGGAAACCT ATGCACTGAG
    GAACAGACTG CACCAGCAAG ACCAGAAGCT TATCCTATCC CTACACAAAC ATATGCAAGA
    GAGTATTTTA CTTTTCCAAC TCCAAAATCT CAAGATAGAA TAGGACCAGC TCAGAACCAA
    TGGCAAGGCT ATGAGGATAA TTTCCAAGTT CAGATGGGTA ACAACCATGC CAGAAATACT
    TCGGCTCAAC AAGTTGCTTA TTCTGAGGAA GAGCTAAGGG ATAATTTAAC ATTCCAGTCA
    GAAAGAGGGC GCGTTGAACC CCAAGTGCGG AAAGATGTGG AACATCTGGA TGGTAATGAT
    ACTTGGTTCA ATCGAGGCTC GTCTATGGAA TCAAGTGCAT CTTGTCAAGA CCTTAATAAT
    TCAAAGACAC ATTCTGTAGG TTCTTTGAGC AAAACAGGAC CTGGTCGCTG GAAAACACCA
    ATTTCTTCTC AGTCAGCTAC TGTTCTTTTG CCGCAGCCTG TTAGAACAGA CCTTCCTCCT
    CCACCACCTC CACCAGTTCA TTATGGAGCA GAATTTGAGG GAGTCCCAAT AGATTTACCT
    CTACCACCCC CACCATCTGA GAGCCAACTA GGGCTTTCAA CAAACTCACA GGCAGCTGCA
    CTAGAAAGAA AGAAGCGGGA AGAACATCAG AGGTGGTATG AGAAAGAGAA GGCACGTTTG
    GAAGAAGAAA GAGAAAGGAA AAGGCGAGAG CAGGAAAGAA AGCTAGGCCA GGTTCGTCCT
    CAGCCACCAA CTATACCTCC ACAACCAGTA AATCCACAAT CTCCTCCGTT GATTCCTCCT
    CCAAATGTTG CACAAGCCAG AACAGAGAAA CCTGCCACTC TTCCTCGGCA ACAAGAAACT
    GTTATCAGGG AGCTACAACC TCAGCAGCAG CCACGAACCA TAGAAAGAAG AGACCTCCAG
    TACATTACAG TCAGTAAAGA GGAGCTTTCT TCCAGTGACA GCCTTTCCCC AGATCCCTGG
    AAAAGGGATG CCAAGGAGAA GCTAGAAAAA CAGCAGCAGA TGCATATAGT GGATATGCTG
    AGCAAAGAGA TCAATGAACT GCAAAACAAG CCTGACCGAA CTTCTGAAGA AAATGACCGA
    CTCCGCAAAT TAATGCTTGA ATGGCAGTTT CAGAAGAGAC TACAAGAGTC CCAGCAGAAG
    GATGACGATG ACGACGATGA CGATGATGAT GATGATGATG TGGATACCAT GCTCATCATG
    CAGCGTCTGG AAGCTGAAAG GAAAGCAAGG GATGAAGAAC GGAGACGGCA ACAACATTTG
    GAAGAGGCGC GCAAACGGGA GGCAGAAGAT CGCCTTAGGC AGGAAGAAGA GCATCGTCAT
    CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT GAAGAAAAGA GGAGACAGGA AGAAGAGTAC
    TACAACCGCT TGGAAGCTGA GAGGCGCAGA ATTCATGAAG AAGCAGAGCG GCGATTGTTA
    GACCCTGAAG AGCCTGGTCT GTACAGACCT CCACTCCCAC GGGAATATGA GCTTTCCTCC
    CCAACCCTCC CACCTAATCC TCCGCCTCCC CCTCCTCAGC GTAACACCTC TTACCTCAAA
    ACACAAGCCC TCTCCCCTGA CACAGTTTAC ACTGCCAAGT TTGTTACATA CACTGATGAT
    GAGGAAGACA CAAACCTATC AGGACCAAAT TCTTATTCTG GATCATCTGG TGCAGCAGTT
    CCTGGTAATG AAGTTTATAA AGATCCGAGG GATGTGCGAC TAAAAAGCCA AGATGGAGAA
    GCTTATAGCA CTGGTGGAGC CCCAGAGAGC CTAACCTTTA AGGAGCGCCA GCGGCTCTTC
    TCTCAGGGCC AGGATGTGTC TAACAAAGTG AAAGCTTCCA GAAAATTAAT GGAACTGGAA
    AATGAGTTAA ACACAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758162.1
    CDS999..6419
    Translation

    Target ORF information:

    RefSeq Version XM_031902302.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902302.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT
    CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA
    AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG
    TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA
    GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG
    GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT
    ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT
    GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA
    CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT
    TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA
    TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA
    GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG
    TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG
    GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT
    TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT
    TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG
    TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA
    GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT
    ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT
    GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA
    AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT
    GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA
    GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA
    CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT
    ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC
    AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT
    GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT
    GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGAAGCA AAAGAATATT
    GCTGGAGCTC TTGCTTTCTG GATGGCAAAC GCTTCAGAGC TTCTCAATTT CATAAAGCAA
    GACCGAGATC TCAATCGTAT CACATTGGAT GCACAAGATG TTCTTGCACA TTTAGTTCAG
    ATGGCATTCA AATACCTGGT TCACTGTCTT CAATCTGAAT TGACCAGCTC TATGCCTGCT
    TTTATAGATG ATCCAGAAGA AAATGATTCC CAAAGGCCTA AAATAGAAGA CGTTTTGCAT
    ACTCTGACAG GTGCCATGTC ACTTCTAAGA CGTTGCCGGG TCAACGCAGC ACTAACTATT
    CAGCTGTTCT CGCAGCTATT CCATTTCATC AACATGTGGC TGTTCAACAG GCTTGTAACA
    GATCCAGACT CTGGTTTATG CTCTCACTAT TGGGGGGCAA TAATTCGGCA GCAGCTTGCA
    CACATTGAGG CATGGGCTGA AAAACAAGGC CTCGAATTAG CTGCAGATTG TCATCTTAGC
    AGAATAGTTC AGGCTACTAC TCTTCTCACT ATGGACAAGT ACTCGCATCA GGATATTCCA
    AATATTAATA ATACATGTTT TAAGCTGAAT TCCTTACAAC TGCAAGCCCT ATTGCAAAAT
    TACCACTGTG CTCCTGATGA ACCGTACATC CCAACGGAGT TGATAGACAG TGTGGTGTCT
    GTAGCTGAAA ACACTGCAGA TGAATTGGCT CGAAGTGATG GCAGAGAAGT TCAGCTTGAG
    GAGGATCCTG ATCTGCAACT CCCTTTCCTT TTGCCTGAAG ATGGTTATTC ATGTGATGTG
    GTTAGAAATA TTCCTAATGG CCTTCAAGAG TTTTTAGAAC CGCTCTGCCA GAGAGGTTTT
    TGCAGACTTG TCCCTCACTC ACGGTCACCA GGCATGTGGA CAATTTACTT TGAAGGAGCT
    GAATTTGAAA ATAATTTGTT GACCGACAAT ACAGACCTGC CATTAAGAAA AGAACCTGAA
    ATAATCACTG TGACACTTAA AAAGCAGAAT GGAATGGGTT TGAGTATTGT TGCAGCAAAG
    GGTGCTGGAC AAGAAAAACT TGGGATTTAC ATTAAGTCTG TGGTGAAAGG CGGTGCTGCA
    GAAGTGGATG GTCGTTTAGC TGCAGGGGAC CAACTTATAA GTGTGGATGG GCAAAGTTTA
    GTTGGTTTGT CCCAAGAAAG AGCTGCAGAG CTTATGACTA GAACTGGGGC CGTAGTAACC
    CTTGAGGTCG CAAAGCAAGG CGCAATATAC CATGGACTTG CCACACTACT AAACCAACCA
    TCTCCTCTCA TGCAAAGAGA TCGACGTGGC TCAGGAAAAC CACGACCAAA GAGTGAAGGT
    TTTGAGCTTT ACAATAATGC TTCCCAAAAT GGGTCTCCTG ATAGCCCCCA GACACAATGG
    CCAGAATACA CCGAACCCAA AAAGTTACCA GGCGATGATA AACTAATGAA AAACCGTGCA
    GATCATCGAT CCAGTCCTAA TGTGGCCAAT CAACCCCAGA GTCCAGGTGG AAAAACTACT
    TATTCAAGCA GCTCAACAGT GAAGATAACA TCTGTCTCCA CTGGAAACCT ATGCACTGAG
    GAACAGACTG CACCAGCAAG ACCAGAAGCT TATCCTATCC CTACACAAAC ATATGCAAGA
    GAGTATTTTA CTTTTCCAAC TCCAAAATCT CAAGATAGAA TAGGACCAGC TCAGAACCAA
    TGGCAAGGCT ATGAGGATAA TTTCCAAGTT CAGATGGGTA ACAACCATGC CAGAAATACT
    TCGGCTCAAC AAGTTGCTTA TTCTGAGGAA GAGCTAAGGG ATAATTTAAC ATTCCAGTCA
    GAAAGAGGGC GCGTTGAACC CCAAGTGCGG AAAGATGTGG AACATCTGGA TGGTAATGAT
    ACTTGGTTCA ATCGAGGCTC GTCTATGGAA TCAAGTGCAT CTTGTCAAGA CCTTAATAAT
    TCAAAGACAC ATTCTGTAGG TTCTTTGAGC AAAACAGGAC CTGGTCGCTG GAAAACACCA
    ATTTCTTCTC AGTCAGCTAC TGTTCTTTTG CCGCAGCCTG TTAGAACAGA CCTTCCTCCT
    CCACCACCTC CACCAGTTCA TTATGGAGCA GAATTTGAGG GAGTCCCAAT AGATTTACCT
    CTACCACCCC CACCATCTGA GAGCCAACTA GGGCTTTCAA CAAACTCACA GGCAGCTGCA
    CTAGAAAGAA AGAAGCGGGA AGAACATCAG AGGTGGTATG AGAAAGAGAA GGCACGTTTG
    GAAGAAGAAA GAGAAAGGAA AAGGCGAGAG CAGGAAAGAA AGCTAGGCCA GGTTCGTCCT
    CAGCCACCAA CTATACCTCC ACAACCAGTA AATCCACAAT CTCCTCCGTT GATTCCTCCT
    CCAAATGTTG CACAAGCCAG AACAGAGAAA CCTGCCACTC TTCCTCGGCA ACAAGAAACT
    GTTATCAGGG AGCTACAACC TCAGCAGCAG CCACGAACCA TAGAAAGAAG AGACCTCCAG
    TACATTACAG TCAGTAAAGA GGAGCTTTCT TCCAGTGACA GCCTTTCCCC AGATCCCTGG
    AAAAGGGATG CCAAGGAGAA GCTAGAAAAA CAGCAGCAGA TGCATATAGT GGATATGCTG
    AGCAAAGAGA TCAATGAACT GCAAAACAAG CCTGACCGAA CTTCTGAAGA AAATGACCGA
    CTCCGCAAAT TAATGCTTGA ATGGCAGTTT CAGAAGAGAC TACAAGAGTC CCAGCAGAAG
    GATGACGATG ACGACGATGA CGATGATGAT GATGATGATG TGGATACCAT GCTCATCATG
    CAGCGTCTGG AAGCTGAAAG GAAAGCAAGG GATGAAGAAC GGAGACGGCA ACAACATTTG
    GAAGAGGCGC GCAAACGGGA GGCAGAAGAT CGCCTTAGGC AGGAAGAAGA GCATCGTCAT
    CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT GAAGAAAAGA GGAGACAGGA AGAAGAGTAC
    TACAACCGCT TGGAAGCTGA GAGGCGCAGA ATTCATGAAG AAGCAGAGCG GCGATTGTTA
    GACCCTGAAG AGCCTGGTCT GTACAGACCT CCACTCCCAC GGGAATATGA GCTTTCCTCC
    CCAACCCTCC CACCTAATCC TCCGCCTCCC CCTCCTCAGC GTAACACCTC TTACCTCAAA
    ACACAAGCCC TCTCCCCTGA CACAGTTTAC ACTGCCAAGT TTGTTACATA CACTGATGAT
    GAGGAAGACA CAAACCTATC AGGACCAAAT TCTTATTCTG GATCATCTGG TGCAGCAGTT
    CCTGGTAATG AAGTTTATAA AGATCCGAGG GATGTGCGAC TAAAAAGCCA AGATGGAGAA
    GCTTATAGCA CTGGTGGAGC CCCAGAGAGC CTAACCTTTA AGGAGCGCCA GCGGCTCTTC
    TCTCAGGGCC AGGATGTGTC TAACAAAGTG AAAGCTTCCA GAAAATTAAT GGAACTGGAA
    AATGAGTTAA ACACAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58622
    Clone ID Related Accession (Same CDS sequence) XM_031902303.1
    Accession Version XM_031902303.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5424bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT
    ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG
    CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT
    CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT
    GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG
    CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT
    ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA
    ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT
    GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT
    AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT
    CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA
    AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG
    TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT
    GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT
    GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT
    TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA
    GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT
    GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA
    AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT
    GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT
    TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA
    ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA
    CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA
    GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA
    TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT
    GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT
    ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT
    GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA
    AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC
    CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT
    ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG
    TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT
    GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT
    AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA
    CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT
    CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA
    CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT
    GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT
    TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT
    CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT
    CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA
    ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC
    CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC
    TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG
    CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC
    CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG
    AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC
    ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGGATGAAG AACGGAGACG GCAACAACAT
    TTGGAAGAGG CGCGCAAACG GGAGGCAGAA GATCGCCTTA GGCAGGAAGA AGAGCATCGT
    CATCAAGAGG AGGAGCGGGC AAGGAGAGAG GCTGAAGAAA AGAGGAGACA GGAAGAAGAG
    TACTACAACC GCTTGGAAGC TGAGAGGCGC AGAATTCATG AAGAAGCAGA GCGGCGATTG
    TTAGACCCTG AAGAGCCTGG TCTGTACAGA CCTCCACTCC CACGGGAATA TGAGCTTTCC
    TCCCCAACCC TCCCACCTAA TCCTCCGCCT CCCCCTCCTC AGCGTAACAC CTCTTACCTC
    AAAACACAAG CCCTCTCCCC TGACACAGTT TACACTGCCA AGTTTGTTAC ATACACTGAT
    GATGAGGAAG ACACAAACCT ATCAGGACCA AATTCTTATT CTGGATCATC TGGTGCAGCA
    GTTCCTGGTA ATGAAGTTTA TAAAGATCCG AGGGATGTGC GACTAAAAAG CCAAGATGGA
    GAAGCTTATA GCACTGGTGG AGCCCCAGAG AGCCTAACCT TTAAGGAGCG CCAGCGGCTC
    TTCTCTCAGG GCCAGGATGT GTCTAACAAA GTGAAAGCTT CCAGAAAATT AATGGAACTG
    GAAAATGAGT TAAACACAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758163.1
    CDS998..6421
    Translation

    Target ORF information:

    RefSeq Version XM_031902303.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902303.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT
    ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG
    CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT
    CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT
    GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG
    CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT
    ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA
    ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT
    GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT
    AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT
    CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA
    AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG
    TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT
    GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT
    GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT
    TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA
    GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT
    GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA
    AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT
    GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT
    TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA
    ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA
    CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA
    GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA
    TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT
    GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT
    ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT
    GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA
    AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC
    CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT
    ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG
    TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT
    GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT
    AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA
    CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT
    CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA
    CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT
    GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT
    TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT
    CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT
    CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA
    ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC
    CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC
    TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG
    CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC
    CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG
    AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC
    ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGGATGAAG AACGGAGACG GCAACAACAT
    TTGGAAGAGG CGCGCAAACG GGAGGCAGAA GATCGCCTTA GGCAGGAAGA AGAGCATCGT
    CATCAAGAGG AGGAGCGGGC AAGGAGAGAG GCTGAAGAAA AGAGGAGACA GGAAGAAGAG
    TACTACAACC GCTTGGAAGC TGAGAGGCGC AGAATTCATG AAGAAGCAGA GCGGCGATTG
    TTAGACCCTG AAGAGCCTGG TCTGTACAGA CCTCCACTCC CACGGGAATA TGAGCTTTCC
    TCCCCAACCC TCCCACCTAA TCCTCCGCCT CCCCCTCCTC AGCGTAACAC CTCTTACCTC
    AAAACACAAG CCCTCTCCCC TGACACAGTT TACACTGCCA AGTTTGTTAC ATACACTGAT
    GATGAGGAAG ACACAAACCT ATCAGGACCA AATTCTTATT CTGGATCATC TGGTGCAGCA
    GTTCCTGGTA ATGAAGTTTA TAAAGATCCG AGGGATGTGC GACTAAAAAG CCAAGATGGA
    GAAGCTTATA GCACTGGTGG AGCCCCAGAG AGCCTAACCT TTAAGGAGCG CCAGCGGCTC
    TTCTCTCAGG GCCAGGATGT GTCTAACAAA GTGAAAGCTT CCAGAAAATT AATGGAACTG
    GAAAATGAGT TAAACACAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35546
    Clone ID Related Accession (Same CDS sequence) XM_012963753.2
    Accession Version XM_012963753.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product afadin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACACGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAATGGGAGT CAAACTTCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT
    ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG
    CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT
    CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT
    GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG
    CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT
    ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA
    ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT
    GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT
    AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT
    CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA
    AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG
    TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT
    GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT
    GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT
    TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA
    GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT
    GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA
    AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT
    GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT
    TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA
    ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA
    CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA
    GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA
    TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT
    GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT
    ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT
    GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA
    AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC
    CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT
    ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG
    TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT
    GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT
    AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA
    CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT
    CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA
    CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT
    GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT
    TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT
    CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT
    CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA
    ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC
    CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC
    TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG
    CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC
    CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG
    AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC
    ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGTTACAGG ATGAAGAACG GAGACGGCAA
    CAACATTTGG AAGAGGCGCG CAAACGGGAG GCAGAAGATC GCCTTAGGCA GGAAGAAGAG
    CATCGTCATC AAGAGGAGGA GCGGGCAAGG AGAGAGGCTG AAGAAAAGAG GAGACAGGAA
    GAAGAGTACT ACAACCGCTT GGAAGCTGAG AGGCGCAGAA TTCATGAAGA AGCAGAGCGG
    CGATTGTTAG ACCCTGAAGA GCCTGGTCTG TACAGACCTC CACTCCCACG GGAATATGAG
    CTTTCCTCCC CAACCCTCCC ACCTAATCCT CCGCCTCCCC CTCCTCAGCG TAACACCTCT
    TACCTCAAAA CACAAGCCCT CTCCCCTGAC ACAGTTTACA CTGCCAAGTT TGTTACATAC
    ACTGATGATG AGGAAGACAC AAACCTATCA GGACCAAATT CTTATTCTGG ATCATCTGGT
    GCAGCAGTTC CTGGTAATGA AGTTTATAAA GATCCGAGGG ATGTGCGACT AAAAAGCCAA
    GATGGAGAAG CTTATAGCAC TGGTGGAGCC CCAGAGAGCC TAACCTTTAA GGAGCGCCAG
    CGGCTCTTCT CTCAGGGCCA GGATGTGTCT AACAAAGTGA AAGCTTCCAG AAAATTAATG
    GAACTGGAAA ATGAGTTAAA CACAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819207.1
    CDS1316..6745
    Translation

    Target ORF information:

    RefSeq Version XM_012963753.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis afadin, adherens junction formation factor (afdn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963753.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
    GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA
    GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA
    GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA
    GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA
    GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC
    GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT
    CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC
    TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA
    ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG
    GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG
    GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT
    GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT
    ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA
    GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT
    GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT
    GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC
    CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA
    TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG
    CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC
    CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG
    TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC
    ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG
    ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACACGT GTTCAAATTT
    GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT
    AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA
    CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT
    CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT
    CAATGGGAGT CAAACTTCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA
    AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA
    AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG
    TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT
    ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT
    ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG
    CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT
    CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT
    GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG
    CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT
    ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA
    ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT
    GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT
    AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT
    CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA
    AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG
    TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT
    GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT
    GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT
    TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA
    GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT
    GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA
    AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT
    GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT
    TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA
    ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA
    CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA
    GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA
    TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT
    GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT
    ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT
    GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA
    AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC
    CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT
    ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG
    TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT
    GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT
    AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA
    CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT
    CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA
    CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT
    GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT
    TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT
    CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT
    CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA
    ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC
    CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC
    TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG
    CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC
    CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG
    AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC
    ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGTTACAGG ATGAAGAACG GAGACGGCAA
    CAACATTTGG AAGAGGCGCG CAAACGGGAG GCAGAAGATC GCCTTAGGCA GGAAGAAGAG
    CATCGTCATC AAGAGGAGGA GCGGGCAAGG AGAGAGGCTG AAGAAAAGAG GAGACAGGAA
    GAAGAGTACT ACAACCGCTT GGAAGCTGAG AGGCGCAGAA TTCATGAAGA AGCAGAGCGG
    CGATTGTTAG ACCCTGAAGA GCCTGGTCTG TACAGACCTC CACTCCCACG GGAATATGAG
    CTTTCCTCCC CAACCCTCCC ACCTAATCCT CCGCCTCCCC CTCCTCAGCG TAACACCTCT
    TACCTCAAAA CACAAGCCCT CTCCCCTGAC ACAGTTTACA CTGCCAAGTT TGTTACATAC
    ACTGATGATG AGGAAGACAC AAACCTATCA GGACCAAATT CTTATTCTGG ATCATCTGGT
    GCAGCAGTTC CTGGTAATGA AGTTTATAAA GATCCGAGGG ATGTGCGACT AAAAAGCCAA
    GATGGAGAAG CTTATAGCAC TGGTGGAGCC CCAGAGAGCC TAACCTTTAA GGAGCGCCAG
    CGGCTCTTCT CTCAGGGCCA GGATGTGTCT AACAAAGTGA AAGCTTCCAG AAAATTAATG
    GAACTGGAAA ATGAGTTAAA CACAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.