mllt4a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021468681.1	XP_021324356.1	afadin isoform X6
XM_021468684.1	XP_021324359.1	afadin isoform X9
XM_021468685.1	XP_021324360.1	afadin isoform X10
XM_021468687.1	XP_021324362.1	afadin isoform X12
XM_021468689.1	XP_021324364.1	afadin isoform X14
XM_021468693.1	XP_021324368.1	afadin isoform X17
XM_021468694.1	XP_021324369.1	afadin isoform X18
XM_017352609.1	XP_017208098.1	afadin isoform X1
XM_017352620.1	XP_017208109.1	afadin isoform X12
XM_017352618.1	XP_017208107.1	afadin isoform X10
XM_017352610.1	XP_017208099.1	afadin isoform X2
XM_017352615.1	XP_017208104.1	afadin isoform X7
XM_021468676.1	XP_021324351.1	afadin isoform X1
XM_021468678.1	XP_021324353.1	afadin isoform X3
XM_021468688.1	XP_021324363.1	afadin isoform X13
XM_021468695.1	XP_021324370.1	afadin isoform X19
XM_017352616.1	XP_017208105.1	afadin isoform X8
XM_017352619.1	XP_017208108.1	afadin isoform X11
XM_017352621.1	XP_017208110.1	afadin isoform X13
XM_021468679.1	XP_021324354.1	afadin isoform X4
XM_017352611.1	XP_017208100.1	afadin isoform X3
XM_017352612.1	XP_017208101.1	afadin isoform X4
XM_017352613.1	XP_017208102.1	afadin isoform X5
XM_017352614.1	XP_017208103.1	afadin isoform X6
XM_017352617.1	XP_017208106.1	afadin isoform X9
XM_021468677.1	XP_021324352.1	afadin isoform X2
XM_021468680.1	XP_021324355.1	afadin isoform X5
XM_021468682.1	XP_021324357.1	afadin isoform X7
XM_021468686.1	XP_021324361.1	afadin isoform X11
XM_021468683.1	XP_021324358.1	afadin isoform X8
XM_021468690.1	XP_021324365.1	afadin isoform X14
XM_021468691.1	XP_021324366.1	afadin isoform X15
XM_021468692.1	XP_021324367.1	afadin isoform X16

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa150774	XM_021468677.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150775	XM_021468678.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150776	XM_021468679.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150777	XM_021468680.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150778	XM_021468681.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150779	XM_021468682.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150780	XM_021468683.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150781	XM_021468684.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150782	XM_021468685.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150783	XM_021468686.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150784	XM_021468687.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150785	XM_021468688.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150786	XM_021468689.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150786	XM_021468690.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150787	XM_021468691.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150788	XM_021468692.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150789	XM_021468693.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150790	XM_021468694.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150791	XM_021468695.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa42826	XM_021468676.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa42820	XM_017352609.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42821	XM_017352610.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42822	XM_017352611.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42823	XM_017352612.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42824	XM_017352613.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42825	XM_017352614.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42827	XM_017352615.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42828	XM_017352616.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42829	XM_017352617.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42831	XM_017352618.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42832	XM_017352619.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42833	XM_017352620.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42834	XM_017352621.1 Latest version!	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

mllt4a ( XM_021468677.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324352.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa150774
Clone ID Related Accession (Same CDS sequence)	XM_021468677.1
Accession Version	XM_021468677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGA CTGCTGTTCC AGCAATCTCT 
GTACTAGACT TGCTGCAGGA CGAGGAGCGA CGACGGAAGC AGCAGCTGGA GGAAATCCGC 
AAGCGCGAAG CAGAAGAGCG AGCCAAACAG GAGGAGGAGA GACGCTGGAG AGAGGAGGAG 
AGAGCCAGGA GAGAGGCTGA CGAGAAGAGG AGACAGGAGG AAGAGTACTA CACCCGCCTG 
GAGGCAGAGC GGCGCCGGCA GCACGAGGAA GCTGAGCGCA AGCTGCCGAC GCCAGATGAG 
ACGGGTCTGT ACAGACCCCC TTTACCCCGG GACTACCAGC CCCCTTCTCC CTCAAGCGCA 
CCCGCCACTA ACCACACCAC CAGCGCCCCC CCGCCTCCGC CTCAGAGAAA CACCTCCTAC 
CTCAAAACCC AGGTCGCCTC CCCAGACACC ATTTACACTG CCAAGTTTGT CTCGTACAAT 
GACGAGGAGG AGGAGGAGGA TGAGGAAGAT GCGGGCCTAA CAGGTCAGGT TAAGCTCTCG 
GCCACCCGCA AGTCCTACGG CGACCTTCCG CCTGCCCCAA AGCCCCAGCC GCCGCCAACC 
GCGCGCAAGC CCCGTCCCGT CTCAGATGGC ATCTTTCTGT CCAACTCATT CCAGCTGCCC 
GCTGGGCTCA ATGCCAACAG TACTGCTCTC AAGACAGGGC AGCCCCCTCT CCCCTTCACC 
AAACCCAGCT TCATCCCATC AGCCAACTCC AAAGGATTTA ATTCATACAC TGGAAACACA 
ACAGGACTAG TATCAGGAGA GGTGTATAAA GACCCGCGAG ACAAGTGGAC CAAAGGCAGC 
CCGGATCAAG AGAACACAAT AGGAGGAGCT CCAGAAAACC TGACCTTCAA GGAGCGCCAG 
AGACTCTTCT CGCAGGGGAA AGAGGTCTCC AATAAAGTCA AAGCGTCCCG AAAGCTGATG 
GAGCTGGAGA ACGAACTCAA CATAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324352.1
CDS	281..6073
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468677.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468677.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGA CTGCTGTTCC AGCAATCTCT 
GTACTAGACT TGCTGCAGGA CGAGGAGCGA CGACGGAAGC AGCAGCTGGA GGAAATCCGC 
AAGCGCGAAG CAGAAGAGCG AGCCAAACAG GAGGAGGAGA GACGCTGGAG AGAGGAGGAG 
AGAGCCAGGA GAGAGGCTGA CGAGAAGAGG AGACAGGAGG AAGAGTACTA CACCCGCCTG 
GAGGCAGAGC GGCGCCGGCA GCACGAGGAA GCTGAGCGCA AGCTGCCGAC GCCAGATGAG 
ACGGGTCTGT ACAGACCCCC TTTACCCCGG GACTACCAGC CCCCTTCTCC CTCAAGCGCA 
CCCGCCACTA ACCACACCAC CAGCGCCCCC CCGCCTCCGC CTCAGAGAAA CACCTCCTAC 
CTCAAAACCC AGGTCGCCTC CCCAGACACC ATTTACACTG CCAAGTTTGT CTCGTACAAT 
GACGAGGAGG AGGAGGAGGA TGAGGAAGAT GCGGGCCTAA CAGGTCAGGT TAAGCTCTCG 
GCCACCCGCA AGTCCTACGG CGACCTTCCG CCTGCCCCAA AGCCCCAGCC GCCGCCAACC 
GCGCGCAAGC CCCGTCCCGT CTCAGATGGC ATCTTTCTGT CCAACTCATT CCAGCTGCCC 
GCTGGGCTCA ATGCCAACAG TACTGCTCTC AAGACAGGGC AGCCCCCTCT CCCCTTCACC 
AAACCCAGCT TCATCCCATC AGCCAACTCC AAAGGATTTA ATTCATACAC TGGAAACACA 
ACAGGACTAG TATCAGGAGA GGTGTATAAA GACCCGCGAG ACAAGTGGAC CAAAGGCAGC 
CCGGATCAAG AGAACACAAT AGGAGGAGCT CCAGAAAACC TGACCTTCAA GGAGCGCCAG 
AGACTCTTCT CGCAGGGGAA AGAGGTCTCC AATAAAGTCA AAGCGTCCCG AAAGCTGATG 
GAGCTGGAGA ACGAACTCAA CATAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468678.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324353.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X3, mRNA.

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CloneID	ODa150775
Clone ID Related Accession (Same CDS sequence)	XM_021468678.1
Accession Version	XM_021468678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAG 
AGGAAACTGG ACTTGGATGA GAAACCACTG GTCGTGCAGC TGAACTGGAA TAAAGACGAC 
AGAGAGGGCC GCTTCGTTCT CAAGAACGAG AATGACATTA TGCCCAAGAG AACACTGACA 
AACGGTCCTG AAAAGGAGAA AGAGGGGGTG ATCCAGAACT TCAAAAGAAC ACTGTCCAAG 
AAGGAGAAGA AGAAAGAGAA GAAGAGGGAG AAGGAGGCTT TTTCCAGAAT CCCAGATGGA 
GACGATCATG TGTTGAACCG CGAGGATGGA GAGAACTCCA GATTAGCAGC TGAGGTTTAT 
AAAGACATGC CGGAGACCAG TTTCACAAGG ACCATTTCTA ACCCCGAGGT GGTGATGAAG 
AGAAGACGAC AGCAGAAGCT GGAGAAGAGG ATGCAGGAGT TTCGGAGTGA AGACGGTCGA 
CCCGACTCAG GAGGCACTCT GAGGATCTAC GCTGATAGTC TGAAGCCCAA CATTCCCTAC 
AAGACCATCC TGCTCTCAAC TACTGACATG GCTGACTTTG CTGTGGCCGA GGCTCTGGAG 
AAATATGGCC TGGAGAAGGA AAACCCTAGA GACTACTGCA TCGCCCAGGT GATTCATGCA 
AATGATAAAG CAGTGAAGGA GACCATATTA GATGACACAG ACTGTCCTCT GCAGATCCTC 
AGAGACTGGA CGAGTGATAA AGAGGCCTTA GTTTTCCAGC TGAAGAAGCG TCCTCCAGAT 
TACGCTCCAA GGAAAGGACG TAAGCCAGAC AACCGGATGG GGCCGCAGCA CGGATCCATT 
CCTCCTGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAG TCACTATGCC 
TACTACTACC GCCATCATGA AGATGGCTCC GACTCCAGGG ACAAACCCAA ACTCTACAGA 
CTACAGCACA GCGTTACTGA GGTCGGATCT GACCGGAGTG AAGATGGAAA CATCCAGCTG 
TTTGGGCCTG GGATTCTCCC TCATCACTGT GACCTTATGC ATGCGGACGG CTTGGTGACC 
ATAACCCCAG CCAATATGGA CGCAGAGACA TTTGTGGATG GACAGCGGAT CGCTGACACT 
ACAGTTCTGC GCAGTGGGGT GACCGTCCAG TTCGGAGCAA CTCACGTCTT CAAATTCGTA 
GACCCCAGTT ATGACCAGAG CATGTCCAAA AGAGACCCCG GGCCCATGAT GAAAGGCAGG 
CACAAATCAG GGAGTGTCCC AGAGACGACC TTTGACCTCC ACGGGGACAT TCACAGTGGC 
TCTGCTCTCC CTACAAGCAA GAGCTCAGGG AAGCTGGAGA TGGAGCGGTC AGGAAACAGC 
AGTGAAAGAG GCCTGGTGAA GCCCATGATT CGTGGAGAGC AGCAGGACTT TAGCAAGCAT 
GATGGAAGGT CTCAGGACGG CCCGGAGCTC ACTCTGCCCG CCAGCATCGA GTTCAGGGAA 
AACTCCGAGG ATGCCTTCCT GTCGGCCATC ATCAACTACA CCAACAGCTC GACAGTGCAC 
TTCAAGCTTT CTCCCACATA CGTGCTGTAC ATGACCTGCC GTTATGTCAT GTCCAGCCAA 
TACCGGCCCG ACATCAGTCC CTCCGAACGC ACACACAAAG TCATCGCCAT TGTCAACAAG 
ATGGTGAGCA TGATGGAAGG AGTCATCCAG GAGATTGCTG AAGGTGATCA GAAACAGAAG 
AACATCGCTG GAGCTCTGGC GTTTTGGATG GCGAACGCTT CTGAGCTGCT GAACTTCATC 
AAACAGGACA AGGATCTGAG TCGCATCACG CTGGATGCTC AGGATGTGCT CGCTCATCTT 
GTTCAGATGG CCTTCAAGTA CCTGGTGCAC TGTCTGCAGT CAGATCTCAA TAACTACATG 
CCAGCTTTCC TGGAAGATCC CGACGAGCAA AACCCACAGA GGCCTAAGAT AGAGGATGTT 
CTGCACACGC TGACGGGTGC CATGTCTCTG CTGCGCCGAT GCCGTGTGAA CGCGGCGCTC 
ACCATCCAAC TGTTTTCGCA GCTCTTCCAC TTCGTCAACA TGTGGCTCTT TAACAAGCTG 
GTGACGGAGG CCGATTCTGG CCTGTGCTCA CACTACTGGG GCGCCATCCT TCGCCAGCAG 
CTCAGTCATA TTGAGGCCTG GGCTGAGAAA CAAGGCTTGG AGCTGGCTGC AGACTGCCAC 
CTCAGCCGCA TAGTCCAGGC GACGACCCTC CTTACTATGG ACAAGTACTC CATGCAAGAT 
GTCCAGAACA TCAACAACAC CTGCTTCAAG CTCAACTCTC TTCAGCTCAA CGCTCTGCTG 
AGCAACTACC ACTGCGCTCC TGACGAACCC TACATTCCTC CTGAGCTGAT TGATCACGTG 
GTGGCCGTGG CCGAAAACAC TGCAGACGAA CTGGCCCGAA GCGACGGGCG GGAGGTTCAG 
CTGGAAGAGG ACCCCGACCT TCAGCTGCCT TTCTTGCTGC CTGAGGACGG CTACTCGTGC 
GATGTGGTGC GCAACATTCC CAACGGATTC CAAGAGTTTC TAGAGCCACT TTGCCAGAGA 
GGTTTCTGTA GATTGACTCC TCATCCACGC TCGCCGGGAA CTTGGACCAT TTATTTTGAA 
GGAGCCAACT GTGACAGTCA CTTCTCAGCC GACACCTCAG AACTGCCAAT GCGGAAGGAA 
CCGGAAATTG TGACCGTGAC TTTGAAGAAA CATAATGGAA TGGGCCTCAG TATTGTGGCG 
GCCAAGGGTG CCGGGCAGGA CAAACTTGGT ATTTACATCA AATCTGTTGT CAAAGGAGGT 
GCAGCAGACA TGGATGGGCG TCTGGCTGCT GGAGACCAGT TGCTTAGCGT GGATGGACGT 
AGTCTTGTCG GCCTCTCGCA GGAGAGAGCG GCAGAATTGA TGACACGAAC TGGGTCTGTT 
GTAACGCTTG AGGTGGCCAA ACAAGGAGCC ATTTATCACG GTCTGGCCAC ACTTCTTAAC 
CAGCCATCCC CCATGATGCC TCGCGCGTCA GACCGTGGAC GTGGCGACAA AGGAAAGCTG 
AGACCAAAGA GTGAGGGCTT TGAGCTCTAC AACAACTCTG TACAGAACGG CTCACCTGAG 
AGCCCACAGG GCACGTGGGA CACCTATCCT GAACCAAAGA AAGAGGACAG GTTGTTGAAG 
AACCGAGCAG ACCATCGATC CAGCCCAAAT GTGGCCAATC AGGCTCAGAG TCCAGGCGGG 
AAGCCTGTTT ATCCGGGAGC TCCAGGAAAG ATCACTTCGG TCTCGACGGG TAACCTCTGC 
CCTGATGAGG AGCCCTCTCC ACCCCGTCCA GAGGCCTATC CCATCCCCAC TCAGACTTAC 
CCTAGGGAAT ACTTCACCTT CCCAGCCTCC AAATCTCAGG ACAGGATGGG CCCTGGCCAG 
CCCTGGCAAA ACAACGAACA AGAGCCTCTT CCCCCTATGG ATAACCACTC CAACAACAGC 
ATGGTTATGC AGCGGGTGGC GCGATCTCAG GAGGAGCTGT GTGACATGCC GGGCGGGTAC 
CCAGGTGAAC GTCTGCGCCA GGAAGATCTA ATGCTCCTCC AGCAGCAGCA GCAGCGTGAA 
GCTGAGTACC AGCGAGGTGT TGGAGAACAC TGGAACCACC AGGTGTCTTC CTCAGTGGAG 
TCCAGCACCT CCAGCCAAGA GCACCTCAAC TTCTCCTCAT CCTCTGGCTC CAGGACCCAG 
GGCAACCACA AGAGTGGCCC AGGCCGCTGG AAGACCCCAC TTGCACCTCA TTCCGGCCCT 
GTCTCCCAAC CCTCCCGTTC AGACCTTCCT CCACCTCCAC CTCCTCCACC AGCACACTAC 
GATTTTGAGC CGCAAACCGA CCTGCCGCTT CCACCGCCAC CTTCAGCTAC ACAACAACAG 
CAGCAGGCTG CAGCCGACCG CAAGAAACGT GAAGACCAAC AGCGCTGGTA TGAGAAGGAA 
AAAGCTCGTC TTGAAGAGGA GCGAGAGAGG AAGAGACGAG ATCAGGAAAG GAAATTGGTG 
CAAATCCGAA ACCCTTCGGT TTCTGGTCCA GTTATGCACA ATAACCAGCA TGGCCCGGTG 
CCACCATCTC CACAAATGCC TCCCATGACA CTTCCCCAAT CATACCTTCC TCCAGTCCAA 
CAACCTCCGC CACCACAACC GGCTCGACCC GATAAACTCT CCAGTCTTCC TCGCCAGCCT 
CCGCAAGACA CCGTAATCCG GGACCTCCTG CCGCAGCAGC AGCCGCGCAC CATCGAGAGA 
CGCGACCTGC AGTACATCAC CATCAGCAAG GAGGAGTTAT CGGCCAGCGA CAGCCTGTCA 
CCCGACCCCT GGAAGAGGGA CGCCCGGGAA AAACTGGAGA AGCAGCAGCA GCTTCACATC 
GTTGACCTGC TGGACAAGGA GATCCAGGAG CTGCAGGCTA AACCCGAGCG CACGGCCGAG 
GACAACGACC GCCTGCGCAA ACTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTGCAGGAG 
TCCAAGCAGA ACGAGGAGGA CGAGGAGGAG GAAGACGATG AAGACGTGGA CACCACGCTC 
ATCATGCAGA GACTGGAGGC CGAGAAGAGA GCACGGCAGA CTGCTGTTCC AGCAATCTCT 
GTACTAGACT TGCTGCAGGA CGAGGAGCGA CGACGGAAGC AGCAGCTGGA GGAAATCCGC 
AAGCGCGAAG CAGAAGAGCG AGCCAAACAG GAGGAGGAGA GACGCTGGAG AGAGGAGGAG 
AGAGCCAGGA GAGAGGCTGA CGAGAAGAGG AGACAGGAGG AAGAGTACTA CACCCGCCTG 
GAGGCAGAGC GGCGCCGGCA GCACGAGGAA GCTGAGCGCA AGCTGCCGAC GCCAGATGAG 
ACGGGTCTGT ACAGACCCCC TTTACCCCGG GACTACCAGC CCCCTTCTCC CTCAAGCGCA 
CCCGCCACTA ACCACACCAC CAGCGCCCCC CCGCCTCCGC CTCAGAGAAA CACCTCCTAC 
CTCAAAACCC AGGTCGCCTC CCCAGACACC ATTTACACTG CCAAGTTTGT CTCGTACAAT 
GACGAGGAGG AGGAGGAGGA TGAGGAAGAT GCGGGCCTAA CAGGTCAGGT TAAGCTCTCG 
GCCACCCGCA AGTCCTACGG CGACCTTCCG CCTGCCCCAA AGCCCCAGCC GCCGCCAACC 
GCGCGCAAGC CCCGTCCCGT CTCAGATGGC ATCTTTCTGT CCAACTCATT CCAGCTGCCC 
GCTGGGCTCA ATGCCAACAG TACTGCTCTC AAGACAGGGC AGCCCCCTCT CCCCTTCACC 
AAACCCAGCT TCATCCCATC AGCCAACTCC AAAGGATTTA ATTCATACAC TGGAAACACA 
ACAGGACTAG TATCAGGAGA GGTGTATAAA GACCCGCGAG ACAAGTGGAC CAAAGGCAGC 
CCGGATCAAG AGAACACAAT AGGAGGAGCT CCAGAAAACC TGACCTTCAA GGAGCGCCAG 
AGACTCTTCT CGCAGGGGAA AGAGGTCTCC AATAAAGTCA AAGCGTCCCG AAAGCTGATG 
GAGCTGGAGA ACGAACTCAA CATAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324353.1
CDS	281..6073
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468678.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468678.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAG 
AGGAAACTGG ACTTGGATGA GAAACCACTG GTCGTGCAGC TGAACTGGAA TAAAGACGAC 
AGAGAGGGCC GCTTCGTTCT CAAGAACGAG AATGACATTA TGCCCAAGAG AACACTGACA 
AACGGTCCTG AAAAGGAGAA AGAGGGGGTG ATCCAGAACT TCAAAAGAAC ACTGTCCAAG 
AAGGAGAAGA AGAAAGAGAA GAAGAGGGAG AAGGAGGCTT TTTCCAGAAT CCCAGATGGA 
GACGATCATG TGTTGAACCG CGAGGATGGA GAGAACTCCA GATTAGCAGC TGAGGTTTAT 
AAAGACATGC CGGAGACCAG TTTCACAAGG ACCATTTCTA ACCCCGAGGT GGTGATGAAG 
AGAAGACGAC AGCAGAAGCT GGAGAAGAGG ATGCAGGAGT TTCGGAGTGA AGACGGTCGA 
CCCGACTCAG GAGGCACTCT GAGGATCTAC GCTGATAGTC TGAAGCCCAA CATTCCCTAC 
AAGACCATCC TGCTCTCAAC TACTGACATG GCTGACTTTG CTGTGGCCGA GGCTCTGGAG 
AAATATGGCC TGGAGAAGGA AAACCCTAGA GACTACTGCA TCGCCCAGGT GATTCATGCA 
AATGATAAAG CAGTGAAGGA GACCATATTA GATGACACAG ACTGTCCTCT GCAGATCCTC 
AGAGACTGGA CGAGTGATAA AGAGGCCTTA GTTTTCCAGC TGAAGAAGCG TCCTCCAGAT 
TACGCTCCAA GGAAAGGACG TAAGCCAGAC AACCGGATGG GGCCGCAGCA CGGATCCATT 
CCTCCTGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAG TCACTATGCC 
TACTACTACC GCCATCATGA AGATGGCTCC GACTCCAGGG ACAAACCCAA ACTCTACAGA 
CTACAGCACA GCGTTACTGA GGTCGGATCT GACCGGAGTG AAGATGGAAA CATCCAGCTG 
TTTGGGCCTG GGATTCTCCC TCATCACTGT GACCTTATGC ATGCGGACGG CTTGGTGACC 
ATAACCCCAG CCAATATGGA CGCAGAGACA TTTGTGGATG GACAGCGGAT CGCTGACACT 
ACAGTTCTGC GCAGTGGGGT GACCGTCCAG TTCGGAGCAA CTCACGTCTT CAAATTCGTA 
GACCCCAGTT ATGACCAGAG CATGTCCAAA AGAGACCCCG GGCCCATGAT GAAAGGCAGG 
CACAAATCAG GGAGTGTCCC AGAGACGACC TTTGACCTCC ACGGGGACAT TCACAGTGGC 
TCTGCTCTCC CTACAAGCAA GAGCTCAGGG AAGCTGGAGA TGGAGCGGTC AGGAAACAGC 
AGTGAAAGAG GCCTGGTGAA GCCCATGATT CGTGGAGAGC AGCAGGACTT TAGCAAGCAT 
GATGGAAGGT CTCAGGACGG CCCGGAGCTC ACTCTGCCCG CCAGCATCGA GTTCAGGGAA 
AACTCCGAGG ATGCCTTCCT GTCGGCCATC ATCAACTACA CCAACAGCTC GACAGTGCAC 
TTCAAGCTTT CTCCCACATA CGTGCTGTAC ATGACCTGCC GTTATGTCAT GTCCAGCCAA 
TACCGGCCCG ACATCAGTCC CTCCGAACGC ACACACAAAG TCATCGCCAT TGTCAACAAG 
ATGGTGAGCA TGATGGAAGG AGTCATCCAG GAGATTGCTG AAGGTGATCA GAAACAGAAG 
AACATCGCTG GAGCTCTGGC GTTTTGGATG GCGAACGCTT CTGAGCTGCT GAACTTCATC 
AAACAGGACA AGGATCTGAG TCGCATCACG CTGGATGCTC AGGATGTGCT CGCTCATCTT 
GTTCAGATGG CCTTCAAGTA CCTGGTGCAC TGTCTGCAGT CAGATCTCAA TAACTACATG 
CCAGCTTTCC TGGAAGATCC CGACGAGCAA AACCCACAGA GGCCTAAGAT AGAGGATGTT 
CTGCACACGC TGACGGGTGC CATGTCTCTG CTGCGCCGAT GCCGTGTGAA CGCGGCGCTC 
ACCATCCAAC TGTTTTCGCA GCTCTTCCAC TTCGTCAACA TGTGGCTCTT TAACAAGCTG 
GTGACGGAGG CCGATTCTGG CCTGTGCTCA CACTACTGGG GCGCCATCCT TCGCCAGCAG 
CTCAGTCATA TTGAGGCCTG GGCTGAGAAA CAAGGCTTGG AGCTGGCTGC AGACTGCCAC 
CTCAGCCGCA TAGTCCAGGC GACGACCCTC CTTACTATGG ACAAGTACTC CATGCAAGAT 
GTCCAGAACA TCAACAACAC CTGCTTCAAG CTCAACTCTC TTCAGCTCAA CGCTCTGCTG 
AGCAACTACC ACTGCGCTCC TGACGAACCC TACATTCCTC CTGAGCTGAT TGATCACGTG 
GTGGCCGTGG CCGAAAACAC TGCAGACGAA CTGGCCCGAA GCGACGGGCG GGAGGTTCAG 
CTGGAAGAGG ACCCCGACCT TCAGCTGCCT TTCTTGCTGC CTGAGGACGG CTACTCGTGC 
GATGTGGTGC GCAACATTCC CAACGGATTC CAAGAGTTTC TAGAGCCACT TTGCCAGAGA 
GGTTTCTGTA GATTGACTCC TCATCCACGC TCGCCGGGAA CTTGGACCAT TTATTTTGAA 
GGAGCCAACT GTGACAGTCA CTTCTCAGCC GACACCTCAG AACTGCCAAT GCGGAAGGAA 
CCGGAAATTG TGACCGTGAC TTTGAAGAAA CATAATGGAA TGGGCCTCAG TATTGTGGCG 
GCCAAGGGTG CCGGGCAGGA CAAACTTGGT ATTTACATCA AATCTGTTGT CAAAGGAGGT 
GCAGCAGACA TGGATGGGCG TCTGGCTGCT GGAGACCAGT TGCTTAGCGT GGATGGACGT 
AGTCTTGTCG GCCTCTCGCA GGAGAGAGCG GCAGAATTGA TGACACGAAC TGGGTCTGTT 
GTAACGCTTG AGGTGGCCAA ACAAGGAGCC ATTTATCACG GTCTGGCCAC ACTTCTTAAC 
CAGCCATCCC CCATGATGCC TCGCGCGTCA GACCGTGGAC GTGGCGACAA AGGAAAGCTG 
AGACCAAAGA GTGAGGGCTT TGAGCTCTAC AACAACTCTG TACAGAACGG CTCACCTGAG 
AGCCCACAGG GCACGTGGGA CACCTATCCT GAACCAAAGA AAGAGGACAG GTTGTTGAAG 
AACCGAGCAG ACCATCGATC CAGCCCAAAT GTGGCCAATC AGGCTCAGAG TCCAGGCGGG 
AAGCCTGTTT ATCCGGGAGC TCCAGGAAAG ATCACTTCGG TCTCGACGGG TAACCTCTGC 
CCTGATGAGG AGCCCTCTCC ACCCCGTCCA GAGGCCTATC CCATCCCCAC TCAGACTTAC 
CCTAGGGAAT ACTTCACCTT CCCAGCCTCC AAATCTCAGG ACAGGATGGG CCCTGGCCAG 
CCCTGGCAAA ACAACGAACA AGAGCCTCTT CCCCCTATGG ATAACCACTC CAACAACAGC 
ATGGTTATGC AGCGGGTGGC GCGATCTCAG GAGGAGCTGT GTGACATGCC GGGCGGGTAC 
CCAGGTGAAC GTCTGCGCCA GGAAGATCTA ATGCTCCTCC AGCAGCAGCA GCAGCGTGAA 
GCTGAGTACC AGCGAGGTGT TGGAGAACAC TGGAACCACC AGGTGTCTTC CTCAGTGGAG 
TCCAGCACCT CCAGCCAAGA GCACCTCAAC TTCTCCTCAT CCTCTGGCTC CAGGACCCAG 
GGCAACCACA AGAGTGGCCC AGGCCGCTGG AAGACCCCAC TTGCACCTCA TTCCGGCCCT 
GTCTCCCAAC CCTCCCGTTC AGACCTTCCT CCACCTCCAC CTCCTCCACC AGCACACTAC 
GATTTTGAGC CGCAAACCGA CCTGCCGCTT CCACCGCCAC CTTCAGCTAC ACAACAACAG 
CAGCAGGCTG CAGCCGACCG CAAGAAACGT GAAGACCAAC AGCGCTGGTA TGAGAAGGAA 
AAAGCTCGTC TTGAAGAGGA GCGAGAGAGG AAGAGACGAG ATCAGGAAAG GAAATTGGTG 
CAAATCCGAA ACCCTTCGGT TTCTGGTCCA GTTATGCACA ATAACCAGCA TGGCCCGGTG 
CCACCATCTC CACAAATGCC TCCCATGACA CTTCCCCAAT CATACCTTCC TCCAGTCCAA 
CAACCTCCGC CACCACAACC GGCTCGACCC GATAAACTCT CCAGTCTTCC TCGCCAGCCT 
CCGCAAGACA CCGTAATCCG GGACCTCCTG CCGCAGCAGC AGCCGCGCAC CATCGAGAGA 
CGCGACCTGC AGTACATCAC CATCAGCAAG GAGGAGTTAT CGGCCAGCGA CAGCCTGTCA 
CCCGACCCCT GGAAGAGGGA CGCCCGGGAA AAACTGGAGA AGCAGCAGCA GCTTCACATC 
GTTGACCTGC TGGACAAGGA GATCCAGGAG CTGCAGGCTA AACCCGAGCG CACGGCCGAG 
GACAACGACC GCCTGCGCAA ACTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTGCAGGAG 
TCCAAGCAGA ACGAGGAGGA CGAGGAGGAG GAAGACGATG AAGACGTGGA CACCACGCTC 
ATCATGCAGA GACTGGAGGC CGAGAAGAGA GCACGGCAGA CTGCTGTTCC AGCAATCTCT 
GTACTAGACT TGCTGCAGGA CGAGGAGCGA CGACGGAAGC AGCAGCTGGA GGAAATCCGC 
AAGCGCGAAG CAGAAGAGCG AGCCAAACAG GAGGAGGAGA GACGCTGGAG AGAGGAGGAG 
AGAGCCAGGA GAGAGGCTGA CGAGAAGAGG AGACAGGAGG AAGAGTACTA CACCCGCCTG 
GAGGCAGAGC GGCGCCGGCA GCACGAGGAA GCTGAGCGCA AGCTGCCGAC GCCAGATGAG 
ACGGGTCTGT ACAGACCCCC TTTACCCCGG GACTACCAGC CCCCTTCTCC CTCAAGCGCA 
CCCGCCACTA ACCACACCAC CAGCGCCCCC CCGCCTCCGC CTCAGAGAAA CACCTCCTAC 
CTCAAAACCC AGGTCGCCTC CCCAGACACC ATTTACACTG CCAAGTTTGT CTCGTACAAT 
GACGAGGAGG AGGAGGAGGA TGAGGAAGAT GCGGGCCTAA CAGGTCAGGT TAAGCTCTCG 
GCCACCCGCA AGTCCTACGG CGACCTTCCG CCTGCCCCAA AGCCCCAGCC GCCGCCAACC 
GCGCGCAAGC CCCGTCCCGT CTCAGATGGC ATCTTTCTGT CCAACTCATT CCAGCTGCCC 
GCTGGGCTCA ATGCCAACAG TACTGCTCTC AAGACAGGGC AGCCCCCTCT CCCCTTCACC 
AAACCCAGCT TCATCCCATC AGCCAACTCC AAAGGATTTA ATTCATACAC TGGAAACACA 
ACAGGACTAG TATCAGGAGA GGTGTATAAA GACCCGCGAG ACAAGTGGAC CAAAGGCAGC 
CCGGATCAAG AGAACACAAT AGGAGGAGCT CCAGAAAACC TGACCTTCAA GGAGCGCCAG 
AGACTCTTCT CGCAGGGGAA AGAGGTCTCC AATAAAGTCA AAGCGTCCCG AAAGCTGATG 
GAGCTGGAGA ACGAACTCAA CATAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468679.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324354.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X4, mRNA.

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CloneID	ODa150776
Clone ID Related Accession (Same CDS sequence)	XM_021468679.1
Accession Version	XM_021468679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGTC TGTACAGACC CCCTTTACCC CGGGACTACC AGCCCCCTTC TCCCTCAAGC 
GCACCCGCCA CTAACCACAC CACCAGCGCC CCCCCGCCTC CGCCTCAGAG AAACACCTCC 
TACCTCAAAA CCCAGGTCGC CTCCCCAGAC ACCATTTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGAGGAGGA GGATGAGGAA GATGCGGGCC TAACAGGTCA GGTTAAGCTC 
TCGGCCACCC GCAAGTCCTA CGGCGACCTT CCGCCTGCCC CAAAGCCCCA GCCGCCGCCA 
ACCGCGCGCA AGCCCCGTCC CGTCTCAGAT GGCATCTTTC TGTCCAACTC ATTCCAGCTG 
CCCGCTGGGC TCAATGCCAA CAGTACTGCT CTCAAGACAG GGCAGCCCCC TCTCCCCTTC 
ACCAAACCCA GCTTCATCCC ATCAGCCAAC TCCAAAGGAT TTAATTCATA CACTGGAAAC 
ACAACAGGAC TAGTATCAGG AGAGGTGTAT AAAGACCCGC GAGACAAGTG GACCAAAGGC 
CCGGATCAAG AGAACACAAT AGGAGGAGCT CCAGAAAACC TGACCTTCAA GGAGCGCCAG 
AGACTCTTCT CGCAGGGGAA AGAGGTCTCC AATAAAGTCA AAGCGTCCCG AAAGCTGATG 
GAGCTGGAGA ACGAACTCAA CATAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324354.1
CDS	283..6075
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468679.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468679.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGTC TGTACAGACC CCCTTTACCC CGGGACTACC AGCCCCCTTC TCCCTCAAGC 
GCACCCGCCA CTAACCACAC CACCAGCGCC CCCCCGCCTC CGCCTCAGAG AAACACCTCC 
TACCTCAAAA CCCAGGTCGC CTCCCCAGAC ACCATTTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGAGGAGGA GGATGAGGAA GATGCGGGCC TAACAGGTCA GGTTAAGCTC 
TCGGCCACCC GCAAGTCCTA CGGCGACCTT CCGCCTGCCC CAAAGCCCCA GCCGCCGCCA 
ACCGCGCGCA AGCCCCGTCC CGTCTCAGAT GGCATCTTTC TGTCCAACTC ATTCCAGCTG 
CCCGCTGGGC TCAATGCCAA CAGTACTGCT CTCAAGACAG GGCAGCCCCC TCTCCCCTTC 
ACCAAACCCA GCTTCATCCC ATCAGCCAAC TCCAAAGGAT TTAATTCATA CACTGGAAAC 
ACAACAGGAC TAGTATCAGG AGAGGTGTAT AAAGACCCGC GAGACAAGTG GACCAAAGGC 
CCGGATCAAG AGAACACAAT AGGAGGAGCT CCAGAAAACC TGACCTTCAA GGAGCGCCAG 
AGACTCTTCT CGCAGGGGAA AGAGGTCTCC AATAAAGTCA AAGCGTCCCG AAAGCTGATG 
GAGCTGGAGA ACGAACTCAA CATAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468680.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324355.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X5, mRNA.

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CloneID	ODa150777
Clone ID Related Accession (Same CDS sequence)	XM_021468680.1
Accession Version	XM_021468680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGAAACAGA AGAACATCGC TGGAGCTCTG 
GCGTTTTGGA TGGCGAACGC TTCTGAGCTG CTGAACTTCA TCAAACAGGA CAAGGATCTG 
AGTCGCATCA CGCTGGATGC TCAGGATGTG CTCGCTCATC TTGTTCAGAT GGCCTTCAAG 
TACCTGGTGC ACTGTCTGCA GTCAGATCTC AATAACTACA TGCCAGCTTT CCTGGAAGAT 
CCCGACGAGC AAAACCCACA GAGGCCTAAG ATAGAGGATG TTCTGCACAC GCTGACGGGT 
GCCATGTCTC TGCTGCGCCG ATGCCGTGTG AACGCGGCGC TCACCATCCA ACTGTTTTCG 
CAGCTCTTCC ACTTCGTCAA CATGTGGCTC TTTAACAAGC TGGTGACGGA GGCCGATTCT 
GGCCTGTGCT CACACTACTG GGGCGCCATC CTTCGCCAGC AGCTCAGTCA TATTGAGGCC 
TGGGCTGAGA AACAAGGCTT GGAGCTGGCT GCAGACTGCC ACCTCAGCCG CATAGTCCAG 
GCGACGACCC TCCTTACTAT GGACAAGTAC TCCATGCAAG ATGTCCAGAA CATCAACAAC 
ACCTGCTTCA AGCTCAACTC TCTTCAGCTC AACGCTCTGC TGAGCAACTA CCACTGCGCT 
CCTGACGAAC CCTACATTCC TCCTGAGCTG ATTGATCACG TGGTGGCCGT GGCCGAAAAC 
ACTGCAGACG AACTGGCCCG AAGCGACGGG CGGGAGGTTC AGCTGGAAGA GGACCCCGAC 
CTTCAGCTGC CTTTCTTGCT GCCTGAGGAC GGCTACTCGT GCGATGTGGT GCGCAACATT 
CCCAACGGAT TCCAAGAGTT TCTAGAGCCA CTTTGCCAGA GAGGTTTCTG TAGATTGACT 
CCTCATCCAC GCTCGCCGGG AACTTGGACC ATTTATTTTG AAGGAGCCAA CTGTGACAGT 
CACTTCTCAG CCGACACCTC AGAACTGCCA ATGCGGAAGG AACCGGAAAT TGTGACCGTG 
ACTTTGAAGA AACATAATGG AATGGGCCTC AGTATTGTGG CGGCCAAGGG TGCCGGGCAG 
GACAAACTTG GTATTTACAT CAAATCTGTT GTCAAAGGAG GTGCAGCAGA CATGGATGGG 
CGTCTGGCTG CTGGAGACCA GTTGCTTAGC GTGGATGGAC GTAGTCTTGT CGGCCTCTCG 
CAGGAGAGAG CGGCAGAATT GATGACACGA ACTGGGTCTG TTGTAACGCT TGAGGTGGCC 
AAACAAGGAG CCATTTATCA CGGTCTGGCC ACACTTCTTA ACCAGCCATC CCCCATGATG 
CCTCGCGCGT CAGACCGTGG ACGTGGCGAC AAAGGAAAGC TGAGACCAAA GAGTGAGGGC 
TTTGAGCTCT ACAACAACTC TGTACAGAAC GGCTCACCTG AGAGCCCACA GGGCACGTGG 
GACACCTATC CTGAACCAAA GAAAGAGGAC AGGTTGTTGA AGAACCGAGC AGACCATCGA 
TCCAGCCCAA ATGTGGCCAA TCAGGCTCAG AGTCCAGGCG GGAAGCCTGT TTATCCGGGA 
GCTCCAGGAA AGATCACTTC GGTCTCGACG GGTAACCTCT GCCCTGATGA GGAGCCCTCT 
CCACCCCGTC CAGAGGCCTA TCCCATCCCC ACTCAGACTT ACCCTAGGGA ATACTTCACC 
TTCCCAGCCT CCAAATCTCA GGACAGGATG GGCCCTGGCC AGCCCTGGCA AAACAACGAA 
CAAGAGCCTC TTCCCCCTAT GGATAACCAC TCCAACAACA GCATGGTTAT GCAGCGGGTG 
GCGCGATCTC AGGAGGAGCT GTGTGACATG CCGGGCGGGT ACCCAGGTGA ACGTCTGCGC 
CAGGAAGATC TAATGCTCCT CCAGCAGCAG CAGCAGCGTG AAGCTGAGTA CCAGCGAGGT 
GTTGGAGAAC ACTGGAACCA CCAGGTGTCT TCCTCAGTGG AGTCCAGCAC CTCCAGCCAA 
GAGCACCTCA ACTTCTCCTC ATCCTCTGGC TCCAGGACCC AGGGCAACCA CAAGAGTGGC 
CCAGGCCGCT GGAAGACCCC ACTTGCACCT CATTCCGGCC CTGTCTCCCA ACCCTCCCGT 
TCAGACCTTC CTCCACCTCC ACCTCCTCCA CCAGCACACT ACGATTTTGA GCCGCAAACC 
GACCTGCCGC TTCCACCGCC ACCTTCAGCT ACACAACAAC AGCAGCAGGC TGCAGCCGAC 
CGCAAGAAAC GTGAAGACCA ACAGCGCTGG TATGAGAAGG AAAAAGCTCG TCTTGAAGAG 
GAGCGAGAGA GGAAGAGACG AGATCAGGAA AGGAAATTGG TGCAAATCCG AAACCCTTCG 
GTTTCTGGTC CAGTTATGCA CAATAACCAG CATGGCCCGG TGCCACCATC TCCACAAATG 
CCTCCCATGA CACTTCCCCA ATCATACCTT CCTCCAGTCC AACAACCTCC GCCACCACAA 
CCGGCTCGAC CCGATAAACT CTCCAGTCTT CCTCGCCAGC CTCCGCAAGA CACCGTAATC 
CGGGACCTCC TGCCGCAGCA GCAGCCGCGC ACCATCGAGA GACGCGACCT GCAGTACATC 
ACCATCAGCA AGGAGGAGTT ATCGGCCAGC GACAGCCTGT CACCCGACCC CTGGAAGAGG 
GACGCCCGGG AAAAACTGGA GAAGCAGCAG CAGCTTCACA TCGTTGACCT GCTGGACAAG 
GAGATCCAGG AGCTGCAGGC TAAACCCGAG CGCACGGCCG AGGACAACGA CCGCCTGCGC 
AAACTCATGC TGGAGTGGCA GTTCCAGAAG AGACTGCAGG AGTCCAAGCA GAACGAGGAG 
GACGAGGAGG AGGAAGACGA TGAAGACGTG GACACCACGC TCATCATGCA GAGACTGGAG 
GCCGAGAAGA GAGCACGGCA GACTGCTGTT CCAGCAATCT CTGTACTAGA CTTGCTGCAG 
GACGAGGAGC GACGACGGAA GCAGCAGCTG GAGGAAATCC GCAAGCGCGA AGCAGAAGAG 
CGAGCCAAAC AGGAGGAGGA GAGACGCTGG AGAGAGGAGG AGAGAGCCAG GAGAGAGGCT 
GACGAGAAGA GGAGACAGGA GGAAGAGTAC TACACCCGCC TGGAGGCAGA GCGGCGCCGG 
CAGCACGAGG AAGCTGAGCG CAAGCTGCCG ACGCCAGATG AGACGGGTCT GTACAGACCC 
CCTTTACCCC GGGACTACCA GCCCCCTTCT CCCTCAAGCG CACCCGCCAC TAACCACACC 
ACCAGCGCCC CCCCGCCTCC GCCTCAGAGA AACACCTCCT ACCTCAAAAC CCAGGTCGCC 
TCCCCAGACA CCATTTACAC TGCCAAGTTT GTCTCGTACA ATGACGAGGA GGAGGAGGAG 
GATGAGGAAG ATGCGGGCCT AACAGGTCAG GTTAAGCTCT CGGCCACCCG CAAGTCCTAC 
GGCGACCTTC CGCCTGCCCC AAAGCCCCAG CCGCCGCCAA CCGCGCGCAA GCCCCGTCCC 
GTCTCAGATG GCATCTTTCT GTCCAACTCA TTCCAGCTGC CCGCTGGGCT CAATGCCAAC 
AGTACTGCTC TCAAGACAGG GCAGCCCCCT CTCCCCTTCA CCAAACCCAG CTTCATCCCA 
TCAGCCAACT CCAAAGGATT TAATTCATAC ACTGGAAACA CAACAGGACT AGTATCAGGA 
GAGGTGTATA AAGACCCGCG AGACAAGTGG ACCAAAGGCA GCCCGGATCA AGAGAACACA 
ATAGGAGGAG CTCCAGAAAA CCTGACCTTC AAGGAGCGCC AGAGACTCTT CTCGCAGGGG 
AAAGAGGTCT CCAATAAAGT CAAAGCGTCC CGAAAGCTGA TGGAGCTGGA GAACGAACTC 
AACATAAAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324355.1
CDS	280..6054
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468680.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468680.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGAAACAGA AGAACATCGC TGGAGCTCTG 
GCGTTTTGGA TGGCGAACGC TTCTGAGCTG CTGAACTTCA TCAAACAGGA CAAGGATCTG 
AGTCGCATCA CGCTGGATGC TCAGGATGTG CTCGCTCATC TTGTTCAGAT GGCCTTCAAG 
TACCTGGTGC ACTGTCTGCA GTCAGATCTC AATAACTACA TGCCAGCTTT CCTGGAAGAT 
CCCGACGAGC AAAACCCACA GAGGCCTAAG ATAGAGGATG TTCTGCACAC GCTGACGGGT 
GCCATGTCTC TGCTGCGCCG ATGCCGTGTG AACGCGGCGC TCACCATCCA ACTGTTTTCG 
CAGCTCTTCC ACTTCGTCAA CATGTGGCTC TTTAACAAGC TGGTGACGGA GGCCGATTCT 
GGCCTGTGCT CACACTACTG GGGCGCCATC CTTCGCCAGC AGCTCAGTCA TATTGAGGCC 
TGGGCTGAGA AACAAGGCTT GGAGCTGGCT GCAGACTGCC ACCTCAGCCG CATAGTCCAG 
GCGACGACCC TCCTTACTAT GGACAAGTAC TCCATGCAAG ATGTCCAGAA CATCAACAAC 
ACCTGCTTCA AGCTCAACTC TCTTCAGCTC AACGCTCTGC TGAGCAACTA CCACTGCGCT 
CCTGACGAAC CCTACATTCC TCCTGAGCTG ATTGATCACG TGGTGGCCGT GGCCGAAAAC 
ACTGCAGACG AACTGGCCCG AAGCGACGGG CGGGAGGTTC AGCTGGAAGA GGACCCCGAC 
CTTCAGCTGC CTTTCTTGCT GCCTGAGGAC GGCTACTCGT GCGATGTGGT GCGCAACATT 
CCCAACGGAT TCCAAGAGTT TCTAGAGCCA CTTTGCCAGA GAGGTTTCTG TAGATTGACT 
CCTCATCCAC GCTCGCCGGG AACTTGGACC ATTTATTTTG AAGGAGCCAA CTGTGACAGT 
CACTTCTCAG CCGACACCTC AGAACTGCCA ATGCGGAAGG AACCGGAAAT TGTGACCGTG 
ACTTTGAAGA AACATAATGG AATGGGCCTC AGTATTGTGG CGGCCAAGGG TGCCGGGCAG 
GACAAACTTG GTATTTACAT CAAATCTGTT GTCAAAGGAG GTGCAGCAGA CATGGATGGG 
CGTCTGGCTG CTGGAGACCA GTTGCTTAGC GTGGATGGAC GTAGTCTTGT CGGCCTCTCG 
CAGGAGAGAG CGGCAGAATT GATGACACGA ACTGGGTCTG TTGTAACGCT TGAGGTGGCC 
AAACAAGGAG CCATTTATCA CGGTCTGGCC ACACTTCTTA ACCAGCCATC CCCCATGATG 
CCTCGCGCGT CAGACCGTGG ACGTGGCGAC AAAGGAAAGC TGAGACCAAA GAGTGAGGGC 
TTTGAGCTCT ACAACAACTC TGTACAGAAC GGCTCACCTG AGAGCCCACA GGGCACGTGG 
GACACCTATC CTGAACCAAA GAAAGAGGAC AGGTTGTTGA AGAACCGAGC AGACCATCGA 
TCCAGCCCAA ATGTGGCCAA TCAGGCTCAG AGTCCAGGCG GGAAGCCTGT TTATCCGGGA 
GCTCCAGGAA AGATCACTTC GGTCTCGACG GGTAACCTCT GCCCTGATGA GGAGCCCTCT 
CCACCCCGTC CAGAGGCCTA TCCCATCCCC ACTCAGACTT ACCCTAGGGA ATACTTCACC 
TTCCCAGCCT CCAAATCTCA GGACAGGATG GGCCCTGGCC AGCCCTGGCA AAACAACGAA 
CAAGAGCCTC TTCCCCCTAT GGATAACCAC TCCAACAACA GCATGGTTAT GCAGCGGGTG 
GCGCGATCTC AGGAGGAGCT GTGTGACATG CCGGGCGGGT ACCCAGGTGA ACGTCTGCGC 
CAGGAAGATC TAATGCTCCT CCAGCAGCAG CAGCAGCGTG AAGCTGAGTA CCAGCGAGGT 
GTTGGAGAAC ACTGGAACCA CCAGGTGTCT TCCTCAGTGG AGTCCAGCAC CTCCAGCCAA 
GAGCACCTCA ACTTCTCCTC ATCCTCTGGC TCCAGGACCC AGGGCAACCA CAAGAGTGGC 
CCAGGCCGCT GGAAGACCCC ACTTGCACCT CATTCCGGCC CTGTCTCCCA ACCCTCCCGT 
TCAGACCTTC CTCCACCTCC ACCTCCTCCA CCAGCACACT ACGATTTTGA GCCGCAAACC 
GACCTGCCGC TTCCACCGCC ACCTTCAGCT ACACAACAAC AGCAGCAGGC TGCAGCCGAC 
CGCAAGAAAC GTGAAGACCA ACAGCGCTGG TATGAGAAGG AAAAAGCTCG TCTTGAAGAG 
GAGCGAGAGA GGAAGAGACG AGATCAGGAA AGGAAATTGG TGCAAATCCG AAACCCTTCG 
GTTTCTGGTC CAGTTATGCA CAATAACCAG CATGGCCCGG TGCCACCATC TCCACAAATG 
CCTCCCATGA CACTTCCCCA ATCATACCTT CCTCCAGTCC AACAACCTCC GCCACCACAA 
CCGGCTCGAC CCGATAAACT CTCCAGTCTT CCTCGCCAGC CTCCGCAAGA CACCGTAATC 
CGGGACCTCC TGCCGCAGCA GCAGCCGCGC ACCATCGAGA GACGCGACCT GCAGTACATC 
ACCATCAGCA AGGAGGAGTT ATCGGCCAGC GACAGCCTGT CACCCGACCC CTGGAAGAGG 
GACGCCCGGG AAAAACTGGA GAAGCAGCAG CAGCTTCACA TCGTTGACCT GCTGGACAAG 
GAGATCCAGG AGCTGCAGGC TAAACCCGAG CGCACGGCCG AGGACAACGA CCGCCTGCGC 
AAACTCATGC TGGAGTGGCA GTTCCAGAAG AGACTGCAGG AGTCCAAGCA GAACGAGGAG 
GACGAGGAGG AGGAAGACGA TGAAGACGTG GACACCACGC TCATCATGCA GAGACTGGAG 
GCCGAGAAGA GAGCACGGCA GACTGCTGTT CCAGCAATCT CTGTACTAGA CTTGCTGCAG 
GACGAGGAGC GACGACGGAA GCAGCAGCTG GAGGAAATCC GCAAGCGCGA AGCAGAAGAG 
CGAGCCAAAC AGGAGGAGGA GAGACGCTGG AGAGAGGAGG AGAGAGCCAG GAGAGAGGCT 
GACGAGAAGA GGAGACAGGA GGAAGAGTAC TACACCCGCC TGGAGGCAGA GCGGCGCCGG 
CAGCACGAGG AAGCTGAGCG CAAGCTGCCG ACGCCAGATG AGACGGGTCT GTACAGACCC 
CCTTTACCCC GGGACTACCA GCCCCCTTCT CCCTCAAGCG CACCCGCCAC TAACCACACC 
ACCAGCGCCC CCCCGCCTCC GCCTCAGAGA AACACCTCCT ACCTCAAAAC CCAGGTCGCC 
TCCCCAGACA CCATTTACAC TGCCAAGTTT GTCTCGTACA ATGACGAGGA GGAGGAGGAG 
GATGAGGAAG ATGCGGGCCT AACAGGTCAG GTTAAGCTCT CGGCCACCCG CAAGTCCTAC 
GGCGACCTTC CGCCTGCCCC AAAGCCCCAG CCGCCGCCAA CCGCGCGCAA GCCCCGTCCC 
GTCTCAGATG GCATCTTTCT GTCCAACTCA TTCCAGCTGC CCGCTGGGCT CAATGCCAAC 
AGTACTGCTC TCAAGACAGG GCAGCCCCCT CTCCCCTTCA CCAAACCCAG CTTCATCCCA 
TCAGCCAACT CCAAAGGATT TAATTCATAC ACTGGAAACA CAACAGGACT AGTATCAGGA 
GAGGTGTATA AAGACCCGCG AGACAAGTGG ACCAAAGGCA GCCCGGATCA AGAGAACACA 
ATAGGAGGAG CTCCAGAAAA CCTGACCTTC AAGGAGCGCC AGAGACTCTT CTCGCAGGGG 
AAAGAGGTCT CCAATAAAGT CAAAGCGTCC CGAAAGCTGA TGGAGCTGGA GAACGAACTC 
AACATAAAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468681.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324356.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X6, mRNA.

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CloneID	ODa150778
Clone ID Related Accession (Same CDS sequence)	XM_021468681.1
Accession Version	XM_021468681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC TGCAGGACGA GGAGCGACGA 
CGGAAGCAGC AGCTGGAGGA AATCCGCAAG CGCGAAGCAG AAGAGCGAGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGA GCCAGGAGAG AGGCTGACGA GAAGAGGAGA 
CAGGAGGAAG AGTACTACAC CCGCCTGGAG GCAGAGCGGC GCCGGCAGCA CGAGGAAGCT 
GAGCGCAAGC TGCCGACGCC AGATGAGACG GGTCTGTACA GACCCCCTTT ACCCCGGGAC 
TACCAGCCCC CTTCTCCCTC AAGCGCACCC GCCACTAACC ACACCACCAG CGCCCCCCCG 
CCTCCGCCTC AGAGAAACAC CTCCTACCTC AAAACCCAGG TCGCCTCCCC AGACACCATT 
TACACTGCCA AGTTTGTCTC GTACAATGAC GAGGAGGAGG AGGAGGATGA GGAAGATGCG 
GGCCTAACAG GTCAGGTTAA GCTCTCGGCC ACCCGCAAGT CCTACGGCGA CCTTCCGCCT 
GCCCCAAAGC CCCAGCCGCC GCCAACCGCG CGCAAGCCCC GTCCCGTCTC AGATGGCATC 
TTTCTGTCCA ACTCATTCCA GCTGCCCGCT GGGCTCAATG CCAACAGTAC TGCTCTCAAG 
ACAGGGCAGC CCCCTCTCCC CTTCACCAAA CCCAGCTTCA TCCCATCAGC CAACTCCAAA 
GGATTTAATT CATACACTGG AAACACAACA GGACTAGTAT CAGGAGAGGT GTATAAAGAC 
CCGCGAGACA AGTGGACCAA AGGCAGCCCG GATCAAGAGA ACACAATAGG AGGAGCTCCA 
GAAAACCTGA CCTTCAAGGA GCGCCAGAGA CTCTTCTCGC AGGGGAAAGA GGTCTCCAAT 
AAAGTCAAAG CGTCCCGAAA GCTGATGGAG CTGGAGAACG AACTCAACAT AAAACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324356.1
CDS	279..6038
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468681.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468681.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC TGCAGGACGA GGAGCGACGA 
CGGAAGCAGC AGCTGGAGGA AATCCGCAAG CGCGAAGCAG AAGAGCGAGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGA GCCAGGAGAG AGGCTGACGA GAAGAGGAGA 
CAGGAGGAAG AGTACTACAC CCGCCTGGAG GCAGAGCGGC GCCGGCAGCA CGAGGAAGCT 
GAGCGCAAGC TGCCGACGCC AGATGAGACG GGTCTGTACA GACCCCCTTT ACCCCGGGAC 
TACCAGCCCC CTTCTCCCTC AAGCGCACCC GCCACTAACC ACACCACCAG CGCCCCCCCG 
CCTCCGCCTC AGAGAAACAC CTCCTACCTC AAAACCCAGG TCGCCTCCCC AGACACCATT 
TACACTGCCA AGTTTGTCTC GTACAATGAC GAGGAGGAGG AGGAGGATGA GGAAGATGCG 
GGCCTAACAG GTCAGGTTAA GCTCTCGGCC ACCCGCAAGT CCTACGGCGA CCTTCCGCCT 
GCCCCAAAGC CCCAGCCGCC GCCAACCGCG CGCAAGCCCC GTCCCGTCTC AGATGGCATC 
TTTCTGTCCA ACTCATTCCA GCTGCCCGCT GGGCTCAATG CCAACAGTAC TGCTCTCAAG 
ACAGGGCAGC CCCCTCTCCC CTTCACCAAA CCCAGCTTCA TCCCATCAGC CAACTCCAAA 
GGATTTAATT CATACACTGG AAACACAACA GGACTAGTAT CAGGAGAGGT GTATAAAGAC 
CCGCGAGACA AGTGGACCAA AGGCAGCCCG GATCAAGAGA ACACAATAGG AGGAGCTCCA 
GAAAACCTGA CCTTCAAGGA GCGCCAGAGA CTCTTCTCGC AGGGGAAAGA GGTCTCCAAT 
AAAGTCAAAG CGTCCCGAAA GCTGATGGAG CTGGAGAACG AACTCAACAT AAAACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468682.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324357.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X7, mRNA.

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CloneID	ODa150779
Clone ID Related Accession (Same CDS sequence)	XM_021468682.1
Accession Version	XM_021468682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGATGGCTC CGACTCCAGG 
GACAAACCCA AACTCTACAG ACTACAGCAC AGCGTTACTG AGGTCGGATC TGACCGGAGT 
GAAGATGGAA ACATCCAGCT GTTTGGGCCT GGGATTCTCC CTCATCACTG TGACCTTATG 
CATGCGGACG GCTTGGTGAC CATAACCCCA GCCAATATGG ACGCAGAGAC ATTTGTGGAT 
GGACAGCGGA TCGCTGACAC TACAGTTCTG CGCAGTGGGG TGACCGTCCA GTTCGGAGCA 
ACTCACGTCT TCAAATTCGT AGACCCCAGT TATGACCAGA GCATGTCCAA AAGAGACCCC 
GGGCCCATGA TGAAAGGCAG GCACAAATCA GGGAGTGTCC CAGAGACGAC CTTTGACCTC 
CACGGGGACA TTCACAGTGG CTCTGCTCTC CCTACAAGCA AGAGCTCAGG GAAGCTGGAG 
ATGGAGCGGT CAGGAAACAG CAGTGAAAGA GGCCTGGTGA AGCCCATGAT TCGTGGAGAG 
CAGCAGGACT TTAGCAAGCA TGATGGAAGG TCTCAGGACG GCCCGGAGCT CACTCTGCCC 
GCCAGCATCG AGTTCAGGGA AAACTCCGAG GATGCCTTCC TGTCGGCCAT CATCAACTAC 
ACCAACAGCT CGACAGTGCA CTTCAAGCTT TCTCCCACAT ACGTGCTGTA CATGACCTGC 
CGTTATGTCA TGTCCAGCCA ATACCGGCCC GACATCAGTC CCTCCGAACG CACACACAAA 
GTCATCGCCA TTGTCAACAA GATGGTGAGC ATGATGGAAG GAGTCATCCA GGAGATTGCT 
GAAGGTGATC AGAAACAGAA GAACATCGCT GGAGCTCTGG CGTTTTGGAT GGCGAACGCT 
TCTGAGCTGC TGAACTTCAT CAAACAGGAC AAGGATCTGA GTCGCATCAC GCTGGATGCT 
CAGGATGTGC TCGCTCATCT TGTTCAGATG GCCTTCAAGT ACCTGGTGCA CTGTCTGCAG 
TCAGATCTCA ATAACTACAT GCCAGCTTTC CTGGAAGATC CCGACGAGCA AAACCCACAG 
AGGCCTAAGA TAGAGGATGT TCTGCACACG CTGACGGGTG CCATGTCTCT GCTGCGCCGA 
TGCCGTGTGA ACGCGGCGCT CACCATCCAA CTGTTTTCGC AGCTCTTCCA CTTCGTCAAC 
ATGTGGCTCT TTAACAAGCT GGTGACGGAG GCCGATTCTG GCCTGTGCTC ACACTACTGG 
GGCGCCATCC TTCGCCAGCA GCTCAGTCAT ATTGAGGCCT GGGCTGAGAA ACAAGGCTTG 
GAGCTGGCTG CAGACTGCCA CCTCAGCCGC ATAGTCCAGG CGACGACCCT CCTTACTATG 
GACAAGTACT CCATGCAAGA TGTCCAGAAC ATCAACAACA CCTGCTTCAA GCTCAACTCT 
CTTCAGCTCA ACGCTCTGCT GAGCAACTAC CACTGCGCTC CTGACGAACC CTACATTCCT 
CCTGAGCTGA TTGATCACGT GGTGGCCGTG GCCGAAAACA CTGCAGACGA ACTGGCCCGA 
AGCGACGGGC GGGAGGTTCA GCTGGAAGAG GACCCCGACC TTCAGCTGCC TTTCTTGCTG 
CCTGAGGACG GCTACTCGTG CGATGTGGTG CGCAACATTC CCAACGGATT CCAAGAGTTT 
CTAGAGCCAC TTTGCCAGAG AGGTTTCTGT AGATTGACTC CTCATCCACG CTCGCCGGGA 
ACTTGGACCA TTTATTTTGA AGGAGCCAAC TGTGACAGTC ACTTCTCAGC CGACACCTCA 
GAACTGCCAA TGCGGAAGGA ACCGGAAATT GTGACCGTGA CTTTGAAGAA ACATAATGGA 
ATGGGCCTCA GTATTGTGGC GGCCAAGGGT GCCGGGCAGG ACAAACTTGG TATTTACATC 
AAATCTGTTG TCAAAGGAGG TGCAGCAGAC ATGGATGGGC GTCTGGCTGC TGGAGACCAG 
TTGCTTAGCG TGGATGGACG TAGTCTTGTC GGCCTCTCGC AGGAGAGAGC GGCAGAATTG 
ATGACACGAA CTGGGTCTGT TGTAACGCTT GAGGTGGCCA AACAAGGAGC CATTTATCAC 
GGTCTGGCCA CACTTCTTAA CCAGCCATCC CCCATGATGC CTCGCGCGTC AGACCGTGGA 
CGTGGCGACA AAGGAAAGCT GAGACCAAAG AGTGAGGGCT TTGAGCTCTA CAACAACTCT 
GTACAGAACG GCTCACCTGA GAGCCCACAG GGCACGTGGG ACACCTATCC TGAACCAAAG 
AAAGAGGACA GGTTGTTGAA GAACCGAGCA GACCATCGAT CCAGCCCAAA TGTGGCCAAT 
CAGGCTCAGA GTCCAGGCGG GAAGCCTGTT TATCCGGGAG CTCCAGGAAA GATCACTTCG 
GTCTCGACGG GTAACCTCTG CCCTGATGAG GAGCCCTCTC CACCCCGTCC AGAGGCCTAT 
CCCATCCCCA CTCAGACTTA CCCTAGGGAA TACTTCACCT TCCCAGCCTC CAAATCTCAG 
GACAGGATGG GCCCTGGCCA GCCCTGGCAA AACAACGAAC AAGAGCCTCT TCCCCCTATG 
GATAACCACT CCAACAACAG CATGGTTATG CAGCGGGTGG CGCGATCTCA GGAGGAGCTG 
TGTGACATGC CGGGCGGGTA CCCAGGTGAA CGTCTGCGCC AGGAAGATCT AATGCTCCTC 
CAGCAGCAGC AGCAGCGTGA AGCTGAGTAC CAGCGAGGTG TTGGAGAACA CTGGAACCAC 
CAGGTGTCTT CCTCAGTGGA GTCCAGCACC TCCAGCCAAG AGCACCTCAA CTTCTCCTCA 
TCCTCTGGCT CCAGGACCCA GGGCAACCAC AAGAGTGGCC CAGGCCGCTG GAAGACCCCA 
CTTGCACCTC ATTCCGGCCC TGTCTCCCAA CCCTCCCGTT CAGACCTTCC TCCACCTCCA 
CCTCCTCCAC CAGCACACTA CGATTTTGAG CCGCAAACCG ACCTGCCGCT TCCACCGCCA 
CCTTCAGCTA CACAACAACA GCAGCAGGCT GCAGCCGACC GCAAGAAACG TGAAGACCAA 
CAGCGCTGGT ATGAGAAGGA AAAAGCTCGT CTTGAAGAGG AGCGAGAGAG GAAGAGACGA 
GATCAGGAAA GGAAATTGGT GCAAATCCGA AACCCTTCGG TTTCTGGTCC AGTTATGCAC 
AATAACCAGC ATGGCCCGGT GCCACCATCT CCACAAATGC CTCCCATGAC ACTTCCCCAA 
TCATACCTTC CTCCAGTCCA ACAACCTCCG CCACCACAAC CGGCTCGACC CGATAAACTC 
TCCAGTCTTC CTCGCCAGCC TCCGCAAGAC ACCGTAATCC GGGACCTCCT GCCGCAGCAG 
CAGCCGCGCA CCATCGAGAG ACGCGACCTG CAGTACATCA CCATCAGCAA GGAGGAGTTA 
TCGGCCAGCG ACAGCCTGTC ACCCGACCCC TGGAAGAGGG ACGCCCGGGA AAAACTGGAG 
AAGCAGCAGC AGCTTCACAT CGTTGACCTG CTGGACAAGG AGATCCAGGA GCTGCAGGCT 
AAACCCGAGC GCACGGCCGA GGACAACGAC CGCCTGCGCA AACTCATGCT GGAGTGGCAG 
TTCCAGAAGA GACTGCAGGA GTCCAAGCAG AACGAGGAGG ACGAGGAGGA GGAAGACGAT 
GAAGACGTGG ACACCACGCT CATCATGCAG AGACTGGAGG CCGAGAAGAG AGCACGGCAG 
ACTGCTGTTC CAGCAATCTC TGTACTAGAC TTGCTGCAGG ACGAGGAGCG ACGACGGAAG 
CAGCAGCTGG AGGAAATCCG CAAGCGCGAA GCAGAAGAGC GAGCCAAACA GGAGGAGGAG 
AGACGCTGGA GAGAGGAGGA GAGAGCCAGG AGAGAGGCTG ACGAGAAGAG GAGACAGGAG 
GAAGAGTACT ACACCCGCCT GGAGGCAGAG CGGCGCCGGC AGCACGAGGA AGCTGAGCGC 
AAGCTGCCGA CGCCAGATGA GACGGGTCTG TACAGACCCC CTTTACCCCG GGACTACCAG 
CCCCCTTCTC CCTCAAGCGC ACCCGCCACT AACCACACCA CCAGCGCCCC CCCGCCTCCG 
CCTCAGAGAA ACACCTCCTA CCTCAAAACC CAGGTCGCCT CCCCAGACAC CATTTACACT 
GCCAAGTTTG TCTCGTACAA TGACGAGGAG GAGGAGGAGG ATGAGGAAGA TGCGGGCCTA 
ACAGGTCAGG TTAAGCTCTC GGCCACCCGC AAGTCCTACG GCGACCTTCC GCCTGCCCCA 
AAGCCCCAGC CGCCGCCAAC CGCGCGCAAG CCCCGTCCCG TCTCAGATGG CATCTTTCTG 
TCCAACTCAT TCCAGCTGCC CGCTGGGCTC AATGCCAACA GTACTGCTCT CAAGACAGGG 
CAGCCCCCTC TCCCCTTCAC CAAACCCAGC TTCATCCCAT CAGCCAACTC CAAAGGATTT 
AATTCATACA CTGGAAACAC AACAGGACTA GTATCAGGAG AGGTGTATAA AGACCCGCGA 
GACAAGTGGA CCAAAGGCAG CCCGGATCAA GAGAACACAA TAGGAGGAGC TCCAGAAAAC 
CTGACCTTCA AGGAGCGCCA GAGACTCTTC TCGCAGGGGA AAGAGGTCTC CAATAAAGTC 
AAAGCGTCCC GAAAGCTGAT GGAGCTGGAG AACGAACTCA ACATAAAACA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324357.1
CDS	280..6033
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468682.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468682.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGATGGCTC CGACTCCAGG 
GACAAACCCA AACTCTACAG ACTACAGCAC AGCGTTACTG AGGTCGGATC TGACCGGAGT 
GAAGATGGAA ACATCCAGCT GTTTGGGCCT GGGATTCTCC CTCATCACTG TGACCTTATG 
CATGCGGACG GCTTGGTGAC CATAACCCCA GCCAATATGG ACGCAGAGAC ATTTGTGGAT 
GGACAGCGGA TCGCTGACAC TACAGTTCTG CGCAGTGGGG TGACCGTCCA GTTCGGAGCA 
ACTCACGTCT TCAAATTCGT AGACCCCAGT TATGACCAGA GCATGTCCAA AAGAGACCCC 
GGGCCCATGA TGAAAGGCAG GCACAAATCA GGGAGTGTCC CAGAGACGAC CTTTGACCTC 
CACGGGGACA TTCACAGTGG CTCTGCTCTC CCTACAAGCA AGAGCTCAGG GAAGCTGGAG 
ATGGAGCGGT CAGGAAACAG CAGTGAAAGA GGCCTGGTGA AGCCCATGAT TCGTGGAGAG 
CAGCAGGACT TTAGCAAGCA TGATGGAAGG TCTCAGGACG GCCCGGAGCT CACTCTGCCC 
GCCAGCATCG AGTTCAGGGA AAACTCCGAG GATGCCTTCC TGTCGGCCAT CATCAACTAC 
ACCAACAGCT CGACAGTGCA CTTCAAGCTT TCTCCCACAT ACGTGCTGTA CATGACCTGC 
CGTTATGTCA TGTCCAGCCA ATACCGGCCC GACATCAGTC CCTCCGAACG CACACACAAA 
GTCATCGCCA TTGTCAACAA GATGGTGAGC ATGATGGAAG GAGTCATCCA GGAGATTGCT 
GAAGGTGATC AGAAACAGAA GAACATCGCT GGAGCTCTGG CGTTTTGGAT GGCGAACGCT 
TCTGAGCTGC TGAACTTCAT CAAACAGGAC AAGGATCTGA GTCGCATCAC GCTGGATGCT 
CAGGATGTGC TCGCTCATCT TGTTCAGATG GCCTTCAAGT ACCTGGTGCA CTGTCTGCAG 
TCAGATCTCA ATAACTACAT GCCAGCTTTC CTGGAAGATC CCGACGAGCA AAACCCACAG 
AGGCCTAAGA TAGAGGATGT TCTGCACACG CTGACGGGTG CCATGTCTCT GCTGCGCCGA 
TGCCGTGTGA ACGCGGCGCT CACCATCCAA CTGTTTTCGC AGCTCTTCCA CTTCGTCAAC 
ATGTGGCTCT TTAACAAGCT GGTGACGGAG GCCGATTCTG GCCTGTGCTC ACACTACTGG 
GGCGCCATCC TTCGCCAGCA GCTCAGTCAT ATTGAGGCCT GGGCTGAGAA ACAAGGCTTG 
GAGCTGGCTG CAGACTGCCA CCTCAGCCGC ATAGTCCAGG CGACGACCCT CCTTACTATG 
GACAAGTACT CCATGCAAGA TGTCCAGAAC ATCAACAACA CCTGCTTCAA GCTCAACTCT 
CTTCAGCTCA ACGCTCTGCT GAGCAACTAC CACTGCGCTC CTGACGAACC CTACATTCCT 
CCTGAGCTGA TTGATCACGT GGTGGCCGTG GCCGAAAACA CTGCAGACGA ACTGGCCCGA 
AGCGACGGGC GGGAGGTTCA GCTGGAAGAG GACCCCGACC TTCAGCTGCC TTTCTTGCTG 
CCTGAGGACG GCTACTCGTG CGATGTGGTG CGCAACATTC CCAACGGATT CCAAGAGTTT 
CTAGAGCCAC TTTGCCAGAG AGGTTTCTGT AGATTGACTC CTCATCCACG CTCGCCGGGA 
ACTTGGACCA TTTATTTTGA AGGAGCCAAC TGTGACAGTC ACTTCTCAGC CGACACCTCA 
GAACTGCCAA TGCGGAAGGA ACCGGAAATT GTGACCGTGA CTTTGAAGAA ACATAATGGA 
ATGGGCCTCA GTATTGTGGC GGCCAAGGGT GCCGGGCAGG ACAAACTTGG TATTTACATC 
AAATCTGTTG TCAAAGGAGG TGCAGCAGAC ATGGATGGGC GTCTGGCTGC TGGAGACCAG 
TTGCTTAGCG TGGATGGACG TAGTCTTGTC GGCCTCTCGC AGGAGAGAGC GGCAGAATTG 
ATGACACGAA CTGGGTCTGT TGTAACGCTT GAGGTGGCCA AACAAGGAGC CATTTATCAC 
GGTCTGGCCA CACTTCTTAA CCAGCCATCC CCCATGATGC CTCGCGCGTC AGACCGTGGA 
CGTGGCGACA AAGGAAAGCT GAGACCAAAG AGTGAGGGCT TTGAGCTCTA CAACAACTCT 
GTACAGAACG GCTCACCTGA GAGCCCACAG GGCACGTGGG ACACCTATCC TGAACCAAAG 
AAAGAGGACA GGTTGTTGAA GAACCGAGCA GACCATCGAT CCAGCCCAAA TGTGGCCAAT 
CAGGCTCAGA GTCCAGGCGG GAAGCCTGTT TATCCGGGAG CTCCAGGAAA GATCACTTCG 
GTCTCGACGG GTAACCTCTG CCCTGATGAG GAGCCCTCTC CACCCCGTCC AGAGGCCTAT 
CCCATCCCCA CTCAGACTTA CCCTAGGGAA TACTTCACCT TCCCAGCCTC CAAATCTCAG 
GACAGGATGG GCCCTGGCCA GCCCTGGCAA AACAACGAAC AAGAGCCTCT TCCCCCTATG 
GATAACCACT CCAACAACAG CATGGTTATG CAGCGGGTGG CGCGATCTCA GGAGGAGCTG 
TGTGACATGC CGGGCGGGTA CCCAGGTGAA CGTCTGCGCC AGGAAGATCT AATGCTCCTC 
CAGCAGCAGC AGCAGCGTGA AGCTGAGTAC CAGCGAGGTG TTGGAGAACA CTGGAACCAC 
CAGGTGTCTT CCTCAGTGGA GTCCAGCACC TCCAGCCAAG AGCACCTCAA CTTCTCCTCA 
TCCTCTGGCT CCAGGACCCA GGGCAACCAC AAGAGTGGCC CAGGCCGCTG GAAGACCCCA 
CTTGCACCTC ATTCCGGCCC TGTCTCCCAA CCCTCCCGTT CAGACCTTCC TCCACCTCCA 
CCTCCTCCAC CAGCACACTA CGATTTTGAG CCGCAAACCG ACCTGCCGCT TCCACCGCCA 
CCTTCAGCTA CACAACAACA GCAGCAGGCT GCAGCCGACC GCAAGAAACG TGAAGACCAA 
CAGCGCTGGT ATGAGAAGGA AAAAGCTCGT CTTGAAGAGG AGCGAGAGAG GAAGAGACGA 
GATCAGGAAA GGAAATTGGT GCAAATCCGA AACCCTTCGG TTTCTGGTCC AGTTATGCAC 
AATAACCAGC ATGGCCCGGT GCCACCATCT CCACAAATGC CTCCCATGAC ACTTCCCCAA 
TCATACCTTC CTCCAGTCCA ACAACCTCCG CCACCACAAC CGGCTCGACC CGATAAACTC 
TCCAGTCTTC CTCGCCAGCC TCCGCAAGAC ACCGTAATCC GGGACCTCCT GCCGCAGCAG 
CAGCCGCGCA CCATCGAGAG ACGCGACCTG CAGTACATCA CCATCAGCAA GGAGGAGTTA 
TCGGCCAGCG ACAGCCTGTC ACCCGACCCC TGGAAGAGGG ACGCCCGGGA AAAACTGGAG 
AAGCAGCAGC AGCTTCACAT CGTTGACCTG CTGGACAAGG AGATCCAGGA GCTGCAGGCT 
AAACCCGAGC GCACGGCCGA GGACAACGAC CGCCTGCGCA AACTCATGCT GGAGTGGCAG 
TTCCAGAAGA GACTGCAGGA GTCCAAGCAG AACGAGGAGG ACGAGGAGGA GGAAGACGAT 
GAAGACGTGG ACACCACGCT CATCATGCAG AGACTGGAGG CCGAGAAGAG AGCACGGCAG 
ACTGCTGTTC CAGCAATCTC TGTACTAGAC TTGCTGCAGG ACGAGGAGCG ACGACGGAAG 
CAGCAGCTGG AGGAAATCCG CAAGCGCGAA GCAGAAGAGC GAGCCAAACA GGAGGAGGAG 
AGACGCTGGA GAGAGGAGGA GAGAGCCAGG AGAGAGGCTG ACGAGAAGAG GAGACAGGAG 
GAAGAGTACT ACACCCGCCT GGAGGCAGAG CGGCGCCGGC AGCACGAGGA AGCTGAGCGC 
AAGCTGCCGA CGCCAGATGA GACGGGTCTG TACAGACCCC CTTTACCCCG GGACTACCAG 
CCCCCTTCTC CCTCAAGCGC ACCCGCCACT AACCACACCA CCAGCGCCCC CCCGCCTCCG 
CCTCAGAGAA ACACCTCCTA CCTCAAAACC CAGGTCGCCT CCCCAGACAC CATTTACACT 
GCCAAGTTTG TCTCGTACAA TGACGAGGAG GAGGAGGAGG ATGAGGAAGA TGCGGGCCTA 
ACAGGTCAGG TTAAGCTCTC GGCCACCCGC AAGTCCTACG GCGACCTTCC GCCTGCCCCA 
AAGCCCCAGC CGCCGCCAAC CGCGCGCAAG CCCCGTCCCG TCTCAGATGG CATCTTTCTG 
TCCAACTCAT TCCAGCTGCC CGCTGGGCTC AATGCCAACA GTACTGCTCT CAAGACAGGG 
CAGCCCCCTC TCCCCTTCAC CAAACCCAGC TTCATCCCAT CAGCCAACTC CAAAGGATTT 
AATTCATACA CTGGAAACAC AACAGGACTA GTATCAGGAG AGGTGTATAA AGACCCGCGA 
GACAAGTGGA CCAAAGGCAG CCCGGATCAA GAGAACACAA TAGGAGGAGC TCCAGAAAAC 
CTGACCTTCA AGGAGCGCCA GAGACTCTTC TCGCAGGGGA AAGAGGTCTC CAATAAAGTC 
AAAGCGTCCC GAAAGCTGAT GGAGCTGGAG AACGAACTCA ACATAAAACA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468683.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324358.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X8, mRNA.

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CloneID	ODa150780
Clone ID Related Accession (Same CDS sequence)	XM_021468683.1
Accession Version	XM_021468683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGTC TGTACAGACC CCCTTTACCC CGGGACTACC AGCCCCCTTC TCCCTCAAGC 
GCACCCGCCA CTAACCACAC CACCAGCGCC CCCCCGCCTC CGCCTCAGAG AAACACCTCC 
TACCTCAAAA CCCAGGTCGC CTCCCCAGAC ACCATTTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGAGGAGGA GGATGAGGAA GATGCGGGCC TAACAGGATT TAATTCATAC 
ACTGGAAACA CAACAGGACT AGTATCAGGA GAGGTGTATA AAGACCCGCG AGACAAGTGG 
ACCAAAGGCA GCCCGGATCA AGAGAACACA ATAGGAGGAG CTCCAGAAAA CCTGACCTTC 
AAGGAGCGCC AGAGACTCTT CTCGCAGGGG AAAGAGGTCT CCAATAAAGT CAAAGCGTCC 
CGAAAGCTGA TGGAGCTGGA GAACGAACTC AACATAAAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324358.1
CDS	280..5844
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468683.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468683.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGTC TGTACAGACC CCCTTTACCC CGGGACTACC AGCCCCCTTC TCCCTCAAGC 
GCACCCGCCA CTAACCACAC CACCAGCGCC CCCCCGCCTC CGCCTCAGAG AAACACCTCC 
TACCTCAAAA CCCAGGTCGC CTCCCCAGAC ACCATTTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGAGGAGGA GGATGAGGAA GATGCGGGCC TAACAGGATT TAATTCATAC 
ACTGGAAACA CAACAGGACT AGTATCAGGA GAGGTGTATA AAGACCCGCG AGACAAGTGG 
ACCAAAGGCA GCCCGGATCA AGAGAACACA ATAGGAGGAG CTCCAGAAAA CCTGACCTTC 
AAGGAGCGCC AGAGACTCTT CTCGCAGGGG AAAGAGGTCT CCAATAAAGT CAAAGCGTCC 
CGAAAGCTGA TGGAGCTGGA GAACGAACTC AACATAAAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468684.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324359.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X9, mRNA.

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CloneID	ODa150781
Clone ID Related Accession (Same CDS sequence)	XM_021468684.1
Accession Version	XM_021468684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGTC TGTACAGACC CCCTTTACCC CGGGACTACC AGCCCCCTTC TCCCTCAAGC 
GCACCCGCCA CTAACCACAC CACCAGCGCC CCCCCGCCTC CGCCTCAGAG AAACACCTCC 
TACCTCAAAA CCCAGGTCGC CTCCCCAGAC ACCATTTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGAGGAGGA GGATGAGGAA GATGCGGGCC TAACAGGATT TAATTCATAC 
ACTGGAAACA CAACAGGACT AGTATCAGGA GAGGTGTATA AAGACCCGCG AGACAAGTGG 
ACCAAAGGCC CGGATCAAGA GAACACAATA GGAGGAGCTC CAGAAAACCT GACCTTCAAG 
GAGCGCCAGA GACTCTTCTC GCAGGGGAAA GAGGTCTCCA ATAAAGTCAA AGCGTCCCGA 
AAGCTGATGG AGCTGGAGAA CGAACTCAAC ATAAAACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324359.1
CDS	282..5843
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468684.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468684.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGTC TGTACAGACC CCCTTTACCC CGGGACTACC AGCCCCCTTC TCCCTCAAGC 
GCACCCGCCA CTAACCACAC CACCAGCGCC CCCCCGCCTC CGCCTCAGAG AAACACCTCC 
TACCTCAAAA CCCAGGTCGC CTCCCCAGAC ACCATTTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGAGGAGGA GGATGAGGAA GATGCGGGCC TAACAGGATT TAATTCATAC 
ACTGGAAACA CAACAGGACT AGTATCAGGA GAGGTGTATA AAGACCCGCG AGACAAGTGG 
ACCAAAGGCC CGGATCAAGA GAACACAATA GGAGGAGCTC CAGAAAACCT GACCTTCAAG 
GAGCGCCAGA GACTCTTCTC GCAGGGGAAA GAGGTCTCCA ATAAAGTCAA AGCGTCCCGA 
AAGCTGATGG AGCTGGAGAA CGAACTCAAC ATAAAACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468685.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324360.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X10, mRNA.

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CloneID	ODa150782
Clone ID Related Accession (Same CDS sequence)	XM_021468685.1
Accession Version	XM_021468685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC TGCAGGACGA GGAGCGACGA 
CGGAAGCAGC AGCTGGAGGA AATCCGCAAG CGCGAAGCAG AAGAGCGAGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGA GCCAGGAGAG AGGCTGACGA GAAGAGGAGA 
CAGGAGGAAG AGTACTACAC CCGCCTGGAG GCAGAGCGGC GCCGGCAGCA CGAGGAAGCT 
GAGCGCAAGC TGCCGACGCC AGATGAGACG GGTCTGTACA GACCCCCTTT ACCCCGGGAC 
TACCAGCCCC CTTCTCCCTC AAGCGCACCC GCCACTAACC ACACCACCAG CGCCCCCCCG 
CCTCCGCCTC AGAGAAACAC CTCCTACCTC AAAACCCAGG TCGCCTCCCC AGACACCATT 
TACACTGCCA AGTTTGTCTC GTACAATGAC GAGGAGGAGG AGGAGGATGA GGAAGATGCG 
GGCCTAACAG GATTTAATTC ATACACTGGA AACACAACAG GACTAGTATC AGGAGAGGTG 
TATAAAGACC CGCGAGACAA GTGGACCAAA GGCCCGGATC AAGAGAACAC AATAGGAGGA 
GCTCCAGAAA ACCTGACCTT CAAGGAGCGC CAGAGACTCT TCTCGCAGGG GAAAGAGGTC 
TCCAATAAAG TCAAAGCGTC CCGAAAGCTG ATGGAGCTGG AGAACGAACT CAACATAAAA 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324360.1
CDS	281..5806
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468685.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468685.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC TGCAGGACGA GGAGCGACGA 
CGGAAGCAGC AGCTGGAGGA AATCCGCAAG CGCGAAGCAG AAGAGCGAGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGA GCCAGGAGAG AGGCTGACGA GAAGAGGAGA 
CAGGAGGAAG AGTACTACAC CCGCCTGGAG GCAGAGCGGC GCCGGCAGCA CGAGGAAGCT 
GAGCGCAAGC TGCCGACGCC AGATGAGACG GGTCTGTACA GACCCCCTTT ACCCCGGGAC 
TACCAGCCCC CTTCTCCCTC AAGCGCACCC GCCACTAACC ACACCACCAG CGCCCCCCCG 
CCTCCGCCTC AGAGAAACAC CTCCTACCTC AAAACCCAGG TCGCCTCCCC AGACACCATT 
TACACTGCCA AGTTTGTCTC GTACAATGAC GAGGAGGAGG AGGAGGATGA GGAAGATGCG 
GGCCTAACAG GATTTAATTC ATACACTGGA AACACAACAG GACTAGTATC AGGAGAGGTG 
TATAAAGACC CGCGAGACAA GTGGACCAAA GGCCCGGATC AAGAGAACAC AATAGGAGGA 
GCTCCAGAAA ACCTGACCTT CAAGGAGCGC CAGAGACTCT TCTCGCAGGG GAAAGAGGTC 
TCCAATAAAG TCAAAGCGTC CCGAAAGCTG ATGGAGCTGG AGAACGAACT CAACATAAAA 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468686.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324361.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X11, mRNA.

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CloneID	ODa150783
Clone ID Related Accession (Same CDS sequence)	XM_021468686.1
Accession Version	XM_021468686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGAT TTAATTCATA CACTGGAAAC ACAACAGGAC TAGTATCAGG AGAGGTGTAT 
AAAGACCCGC GAGACAAGTG GACCAAAGGC AGCCCGGATC AAGAGAACAC AATAGGAGGA 
GCTCCAGAAA ACCTGACCTT CAAGGAGCGC CAGAGACTCT TCTCGCAGGG GAAAGAGGTC 
TCCAATAAAG TCAAAGCGTC CCGAAAGCTG ATGGAGCTGG AGAACGAACT CAACATAAAA 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324361.1
CDS	280..5625
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468686.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468686.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGAT TTAATTCATA CACTGGAAAC ACAACAGGAC TAGTATCAGG AGAGGTGTAT 
AAAGACCCGC GAGACAAGTG GACCAAAGGC AGCCCGGATC AAGAGAACAC AATAGGAGGA 
GCTCCAGAAA ACCTGACCTT CAAGGAGCGC CAGAGACTCT TCTCGCAGGG GAAAGAGGTC 
TCCAATAAAG TCAAAGCGTC CCGAAAGCTG ATGGAGCTGG AGAACGAACT CAACATAAAA 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468687.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324362.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X12, mRNA.

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CloneID	ODa150784
Clone ID Related Accession (Same CDS sequence)	XM_021468687.1
Accession Version	XM_021468687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGAT TTAATTCATA CACTGGAAAC ACAACAGGAC TAGTATCAGG AGAGGTGTAT 
AAAGACCCGC GAGACAAGTG GACCAAAGGC CCGGATCAAG AGAACACAAT AGGAGGAGCT 
CCAGAAAACC TGACCTTCAA GGAGCGCCAG AGACTCTTCT CGCAGGGGAA AGAGGTCTCC 
AATAAAGTCA AAGCGTCCCG AAAGCTGATG GAGCTGGAGA ACGAACTCAA CATAAAACAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324362.1
CDS	283..5625
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468687.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468687.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGAT TTAATTCATA CACTGGAAAC ACAACAGGAC TAGTATCAGG AGAGGTGTAT 
AAAGACCCGC GAGACAAGTG GACCAAAGGC CCGGATCAAG AGAACACAAT AGGAGGAGCT 
CCAGAAAACC TGACCTTCAA GGAGCGCCAG AGACTCTTCT CGCAGGGGAA AGAGGTCTCC 
AATAAAGTCA AAGCGTCCCG AAAGCTGATG GAGCTGGAGA ACGAACTCAA CATAAAACAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468688.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324363.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X13, mRNA.

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CloneID	ODa150785
Clone ID Related Accession (Same CDS sequence)	XM_021468688.1
Accession Version	XM_021468688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC TGCAGGACGA GGAGCGACGA 
CGGAAGCAGC AGCTGGAGGA AATCCGCAAG CGCGAAGCAG AAGAGCGAGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGA GCCAGGAGAG AGGCTGACGA GAAGAGGAGA 
CAGGAGGAAG AGTACTACAC CCGCCTGGAG GCAGAGCGGC GCCGGCAGCA CGAGGAAGCT 
GAGCGCAAGC TGCCGACGCC AGATGAGACG GGATTTAATT CATACACTGG AAACACAACA 
GGACTAGTAT CAGGAGAGGT GTATAAAGAC CCGCGAGACA AGTGGACCAA AGGCCCGGAT 
CAAGAGAACA CAATAGGAGG AGCTCCAGAA AACCTGACCT TCAAGGAGCG CCAGAGACTC 
TTCTCGCAGG GGAAAGAGGT CTCCAATAAA GTCAAAGCGT CCCGAAAGCT GATGGAGCTG 
GAGAACGAAC TCAACATAAA ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324363.1
CDS	281..5587
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468688.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468688.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC TGCAGGACGA GGAGCGACGA 
CGGAAGCAGC AGCTGGAGGA AATCCGCAAG CGCGAAGCAG AAGAGCGAGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGA GCCAGGAGAG AGGCTGACGA GAAGAGGAGA 
CAGGAGGAAG AGTACTACAC CCGCCTGGAG GCAGAGCGGC GCCGGCAGCA CGAGGAAGCT 
GAGCGCAAGC TGCCGACGCC AGATGAGACG GGATTTAATT CATACACTGG AAACACAACA 
GGACTAGTAT CAGGAGAGGT GTATAAAGAC CCGCGAGACA AGTGGACCAA AGGCCCGGAT 
CAAGAGAACA CAATAGGAGG AGCTCCAGAA AACCTGACCT TCAAGGAGCG CCAGAGACTC 
TTCTCGCAGG GGAAAGAGGT CTCCAATAAA GTCAAAGCGT CCCGAAAGCT GATGGAGCTG 
GAGAACGAAC TCAACATAAA ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468689.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324364.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X14, mRNA.

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CloneID	ODa150786
Clone ID Related Accession (Same CDS sequence)	XM_021468689.1 , XM_021468690.1
Accession Version	XM_021468689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG GATTTAATTC ATACACTGGA AACACAACAG 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324364.1
CDS	283..5328
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKDLIHTLETQQD

Target ORF information:

RefSeq Version	XM_021468689.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468689.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG GATTTAATTC ATACACTGGA AACACAACAG 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468690.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324365.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X15, mRNA.

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CloneID	ODa150786
Clone ID Related Accession (Same CDS sequence)	XM_021468689.1 , XM_021468690.1
Accession Version	XM_021468690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG GATTTAATTC ATACACTGGA AACACAACAG 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324365.1
CDS	284..5329
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKDLIHTLETQQD

Target ORF information:

RefSeq Version	XM_021468690.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468690.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG GATTTAATTC ATACACTGGA AACACAACAG 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468691.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324366.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X16, mRNA.

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CloneID	ODa150787
Clone ID Related Accession (Same CDS sequence)	XM_021468691.1
Accession Version	XM_021468691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAG 
AGGAAACTGG ACTTGGATGA GAAACCACTG GTCGTGCAGC TGAACTGGAA TAAAGACGAC 
AGAGAGGGCC GCTTCGTTCT CAAGAACGAG AATGACATTA TGCCCAAGAG AACACTGACA 
AACGGTCCTG AAAAGGAGAA AGAGGGGGTG ATCCAGAACT TCAAAAGAAC ACTGTCCAAG 
AAGGAGAAGA AGAAAGAGAA GAAGAGGGAG AAGGAGGCTT TTTCCAGAAT CCCAGATGGA 
GACGATCATG TGTTGAACCG CGAGGATGGA GAGAACTCCA GATTAGCAGC TGAGGTTTAT 
AAAGACATGC CGGAGACCAG TTTCACAAGG ACCATTTCTA ACCCCGAGGT GGTGATGAAG 
AGAAGACGAC AGCAGAAGCT GGAGAAGAGG ATGCAGGAGT TTCGGAGTGA AGACGGTCGA 
CCCGACTCAG GAGGCACTCT GAGGATCTAC GCTGATAGTC TGAAGCCCAA CATTCCCTAC 
AAGACCATCC TGCTCTCAAC TACTGACATG GCTGACTTTG CTGTGGCCGA GGCTCTGGAG 
AAATATGGCC TGGAGAAGGA AAACCCTAGA GACTACTGCA TCGCCCAGGT GATTCATGCA 
AATGATAAAG CAGTGAAGGA GACCATATTA GATGACACAG ACTGTCCTCT GCAGATCCTC 
AGAGACTGGA CGAGTGATAA AGAGGCCTTA GTTTTCCAGC TGAAGAAGCG TCCTCCAGAT 
TACGCTCCAA GGAAAGGACG TAAGCCAGAC AACCGGATGG GGCCGCAGCA CGGATCCATT 
CCTCCTGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAG TCACTATGCC 
TACTACTACC GCCATCATGA AGATGGCTCC GACTCCAGGG ACAAACCCAA ACTCTACAGA 
CTACAGCACA GCGTTACTGA GGTCGGATCT GACCGGAGTG AAGATGGAAA CATCCAGCTG 
TTTGGGCCTG GGATTCTCCC TCATCACTGT GACCTTATGC ATGCGGACGG CTTGGTGACC 
ATAACCCCAG CCAATATGGA CGCAGAGACA TTTGTGGATG GACAGCGGAT CGCTGACACT 
ACAGTTCTGC GCAGTGGGGT GACCGTCCAG TTCGGAGCAA CTCACGTCTT CAAATTCGTA 
GACCCCAGTT ATGACCAGAG CATGTCCAAA AGAGACCCCG GGCCCATGAT GAAAGGCAGG 
CACAAATCAG GGAGTGTCCC AGAGACGACC TTTGACCTCC ACGGGGACAT TCACAGTGGC 
TCTGCTCTCC CTACAAGCAA GAGCTCAGGG AAGCTGGAGA TGGAGCGGTC AGGAAACAGC 
AGTGAAAGAG GCCTGGTGAA GCCCATGATT CGTGGAGAGC AGCAGGACTT TAGCAAGCAT 
GATGGAAGGT CTCAGGACGG CCCGGAGCTC ACTCTGCCCG CCAGCATCGA GTTCAGGGAA 
AACTCCGAGG ATGCCTTCCT GTCGGCCATC ATCAACTACA CCAACAGCTC GACAGTGCAC 
TTCAAGCTTT CTCCCACATA CGTGCTGTAC ATGACCTGCC GTTATGTCAT GTCCAGCCAA 
TACCGGCCCG ACATCAGTCC CTCCGAACGC ACACACAAAG TCATCGCCAT TGTCAACAAG 
ATGGTGAGCA TGATGGAAGG AGTCATCCAG AAACAGAAGA ACATCGCTGG AGCTCTGGCG 
TTTTGGATGG CGAACGCTTC TGAGCTGCTG AACTTCATCA AACAGGACAA GGATCTGAGT 
CGCATCACGC TGGATGCTCA GGATGTGCTC GCTCATCTTG TTCAGATGGC CTTCAAGTAC 
CTGGTGCACT GTCTGCAGTC AGATCTCAAT AACTACATGC CAGCTTTCCT GGAAGATCCC 
GACGAGCAAA ACCCACAGAG GCCTAAGATA GAGGATGTTC TGCACACGCT GACGGGTGCC 
ATGTCTCTGC TGCGCCGATG CCGTGTGAAC GCGGCGCTCA CCATCCAACT GTTTTCGCAG 
CTCTTCCACT TCGTCAACAT GTGGCTCTTT AACAAGCTGG TGACGGAGGC CGATTCTGGC 
CTGTGCTCAC ACTACTGGGG CGCCATCCTT CGCCAGCAGC TCAGTCATAT TGAGGCCTGG 
GCTGAGAAAC AAGGCTTGGA GCTGGCTGCA GACTGCCACC TCAGCCGCAT AGTCCAGGCG 
ACGACCCTCC TTACTATGGA CAAGTACTCC ATGCAAGATG TCCAGAACAT CAACAACACC 
TGCTTCAAGC TCAACTCTCT TCAGCTCAAC GCTCTGCTGA GCAACTACCA CTGCGCTCCT 
GACGAACCCT ACATTCCTCC TGAGCTGATT GATCACGTGG TGGCCGTGGC CGAAAACACT 
GCAGACGAAC TGGCCCGAAG CGACGGGCGG GAGGTTCAGC TGGAAGAGGA CCCCGACCTT 
CAGCTGCCTT TCTTGCTGCC TGAGGACGGC TACTCGTGCG ATGTGGTGCG CAACATTCCC 
AACGGATTCC AAGAGTTTCT AGAGCCACTT TGCCAGAGAG GTTTCTGTAG ATTGACTCCT 
CATCCACGCT CGCCGGGAAC TTGGACCATT TATTTTGAAG GAGCCAACTG TGACAGTCAC 
TTCTCAGCCG ACACCTCAGA ACTGCCAATG CGGAAGGAAC CGGAAATTGT GACCGTGACT 
TTGAAGAAAC ATAATGGAAT GGGCCTCAGT ATTGTGGCGG CCAAGGGTGC CGGGCAGGAC 
AAACTTGGTA TTTACATCAA ATCTGTTGTC AAAGGAGGTG CAGCAGACAT GGATGGGCGT 
CTGGCTGCTG GAGACCAGTT GCTTAGCGTG GATGGACGTA GTCTTGTCGG CCTCTCGCAG 
GAGAGAGCGG CAGAATTGAT GACACGAACT GGGTCTGTTG TAACGCTTGA GGTGGCCAAA 
CAAGGAGCCA TTTATCACGG TCTGGCCACA CTTCTTAACC AGCCATCCCC CATGATGCCT 
CGCGCGTCAG ACCGTGGACG TGGCGACAAA GGAAAGCTGA GACCAAAGAG TGAGGGCTTT 
GAGCTCTACA ACAACTCTGT ACAGAACGGC TCACCTGAGA GCCCACAGGG CACGTGGGAC 
ACCTATCCTG AACCAAAGAA AGAGGACAGG TTGTTGAAGA ACCGAGCAGA CCATCGATCC 
AGCCCAAATG TGGCCAATCA GGCTCAGAGT CCAGGCGGGA AGCCTGTTTA TCCGGGAGCT 
CCAGGAAAGA TCACTTCGGT CTCGACGGGT AACCTCTGCC CTGATGAGGA GCCCTCTCCA 
CCCCGTCCAG AGGCCTATCC CATCCCCACT CAGACTTACC CTAGGGAATA CTTCACCTTC 
CCAGCCTCCA AATCTCAGGA CAGGATGGGC CCTGGCCAGC CCTGGCAAAA CAACGAACAA 
GAGCCTCTTC CCCCTATGGA TAACCACTCC AACAACAGCA TGGTTATGCA GCGGGTGGCG 
CGATCTCAGG AGGAGCTGTG TGACATGCCG GGCGGGTACC CAGGTGAACG TCTGCGCCAG 
GAAGATCTAA TGCTCCTCCA GCAGCAGCAG CAGCGTGAAG CTGAGTACCA GCGAGGTGTT 
GGAGAACACT GGAACCACCA GGTGTCTTCC TCAGTGGAGT CCAGCACCTC CAGCCAAGAG 
CACCTCAACT TCTCCTCATC CTCTGGCTCC AGGACCCAGG GCAACCACAA GAGTGGCCCA 
GGCCGCTGGA AGACCCCACT TGCACCTCAT TCCGGCCCTG TCTCCCAACC CTCCCGTTCA 
GACCTTCCTC CACCTCCACC TCCTCCACCA GCACACTACG ATTTTGAGCC GCAAACCGAC 
CTGCCGCTTC CACCGCCACC TTCAGCTACA CAACAACAGC AGCAGGCTGC AGCCGACCGC 
AAGAAACGTG AAGACCAACA GCGCTGGTAT GAGAAGGAAA AAGCTCGTCT TGAAGAGGAG 
CGAGAGAGGA AGAGACGAGA TCAGGAAAGG AAATTGGTGC AAATCCGAAA CCCTTCGGTT 
TCTGGTCCAG TTATGCACAA TAACCAGCAT GGCCCGGTGC CACCATCTCC ACAAATGCCT 
CCCATGACAC TTCCCCAATC ATACCTTCCT CCAGTCCAAC AACCTCCGCC ACCACAACCG 
GCTCGACCCG ATAAACTCTC CAGTCTTCCT CGCCAGCCTC CGCAAGACAC CGTAATCCGG 
GACCTCCTGC CGCAGCAGCA GCCGCGCACC ATCGAGAGAC GCGACCTGCA GTACATCACC 
ATCAGCAAGG AGGAGTTATC GGCCAGCGAC AGCCTGTCAC CCGACCCCTG GAAGAGGGAC 
GCCCGGGAAA AACTGGAGAA GCAGCAGCAG CTTCACATCG TTGACCTGCT GGACAAGGAG 
ATCCAGGAGC TGCAGGCTAA ACCCGAGCGC ACGGCCGAGG ACAACGACCG CCTGCGCAAA 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTGCAGGAGT CCAAGCAGAA CGAGGAGGAC 
GAGGAGGAGG AAGACGATGA AGACGTGGAC ACCACGCTCA TCATGCAGAG ACTGGAGGCC 
GAGAAGAGAG CACGGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGTC TGTACAGACC CCCTTTACCC CGGGACTACC AGCCCCCTTC TCCCTCAAGC 
GCACCCGCCA CTAACCACAC CACCAGCGCC CCCCCGCCTC CGCCTCAGAG AAACACCTCC 
TACCTCAAAA CCCAGGTCGC CTCCCCAGAC ACCATTTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGAGGAGGA GGATGAGGAA GATGCGGGCC TAACAGGATT TAATTCATAC 
ACTGGAAACA CAACAGGACT AGTATCAGGA GAGGTGTATA AAGACCCGCG AGACAAGTGG 
ACCAAAGGCC CGGATCAAGA GAACACAATA GGAGGAGCTC CAGAAAACCT GACCTTCAAG 
GAGCGCCAGA GACTCTTCTC GCAGGGGAAA GAGGTCTCCA ATAAAGTCAA AGCGTCCCGA 
AAGCTGATGG AGCTGGAGAA CGAACTCAAC ATAAAACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324366.1
CDS	280..5781
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468691.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468691.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAG 
AGGAAACTGG ACTTGGATGA GAAACCACTG GTCGTGCAGC TGAACTGGAA TAAAGACGAC 
AGAGAGGGCC GCTTCGTTCT CAAGAACGAG AATGACATTA TGCCCAAGAG AACACTGACA 
AACGGTCCTG AAAAGGAGAA AGAGGGGGTG ATCCAGAACT TCAAAAGAAC ACTGTCCAAG 
AAGGAGAAGA AGAAAGAGAA GAAGAGGGAG AAGGAGGCTT TTTCCAGAAT CCCAGATGGA 
GACGATCATG TGTTGAACCG CGAGGATGGA GAGAACTCCA GATTAGCAGC TGAGGTTTAT 
AAAGACATGC CGGAGACCAG TTTCACAAGG ACCATTTCTA ACCCCGAGGT GGTGATGAAG 
AGAAGACGAC AGCAGAAGCT GGAGAAGAGG ATGCAGGAGT TTCGGAGTGA AGACGGTCGA 
CCCGACTCAG GAGGCACTCT GAGGATCTAC GCTGATAGTC TGAAGCCCAA CATTCCCTAC 
AAGACCATCC TGCTCTCAAC TACTGACATG GCTGACTTTG CTGTGGCCGA GGCTCTGGAG 
AAATATGGCC TGGAGAAGGA AAACCCTAGA GACTACTGCA TCGCCCAGGT GATTCATGCA 
AATGATAAAG CAGTGAAGGA GACCATATTA GATGACACAG ACTGTCCTCT GCAGATCCTC 
AGAGACTGGA CGAGTGATAA AGAGGCCTTA GTTTTCCAGC TGAAGAAGCG TCCTCCAGAT 
TACGCTCCAA GGAAAGGACG TAAGCCAGAC AACCGGATGG GGCCGCAGCA CGGATCCATT 
CCTCCTGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAG TCACTATGCC 
TACTACTACC GCCATCATGA AGATGGCTCC GACTCCAGGG ACAAACCCAA ACTCTACAGA 
CTACAGCACA GCGTTACTGA GGTCGGATCT GACCGGAGTG AAGATGGAAA CATCCAGCTG 
TTTGGGCCTG GGATTCTCCC TCATCACTGT GACCTTATGC ATGCGGACGG CTTGGTGACC 
ATAACCCCAG CCAATATGGA CGCAGAGACA TTTGTGGATG GACAGCGGAT CGCTGACACT 
ACAGTTCTGC GCAGTGGGGT GACCGTCCAG TTCGGAGCAA CTCACGTCTT CAAATTCGTA 
GACCCCAGTT ATGACCAGAG CATGTCCAAA AGAGACCCCG GGCCCATGAT GAAAGGCAGG 
CACAAATCAG GGAGTGTCCC AGAGACGACC TTTGACCTCC ACGGGGACAT TCACAGTGGC 
TCTGCTCTCC CTACAAGCAA GAGCTCAGGG AAGCTGGAGA TGGAGCGGTC AGGAAACAGC 
AGTGAAAGAG GCCTGGTGAA GCCCATGATT CGTGGAGAGC AGCAGGACTT TAGCAAGCAT 
GATGGAAGGT CTCAGGACGG CCCGGAGCTC ACTCTGCCCG CCAGCATCGA GTTCAGGGAA 
AACTCCGAGG ATGCCTTCCT GTCGGCCATC ATCAACTACA CCAACAGCTC GACAGTGCAC 
TTCAAGCTTT CTCCCACATA CGTGCTGTAC ATGACCTGCC GTTATGTCAT GTCCAGCCAA 
TACCGGCCCG ACATCAGTCC CTCCGAACGC ACACACAAAG TCATCGCCAT TGTCAACAAG 
ATGGTGAGCA TGATGGAAGG AGTCATCCAG AAACAGAAGA ACATCGCTGG AGCTCTGGCG 
TTTTGGATGG CGAACGCTTC TGAGCTGCTG AACTTCATCA AACAGGACAA GGATCTGAGT 
CGCATCACGC TGGATGCTCA GGATGTGCTC GCTCATCTTG TTCAGATGGC CTTCAAGTAC 
CTGGTGCACT GTCTGCAGTC AGATCTCAAT AACTACATGC CAGCTTTCCT GGAAGATCCC 
GACGAGCAAA ACCCACAGAG GCCTAAGATA GAGGATGTTC TGCACACGCT GACGGGTGCC 
ATGTCTCTGC TGCGCCGATG CCGTGTGAAC GCGGCGCTCA CCATCCAACT GTTTTCGCAG 
CTCTTCCACT TCGTCAACAT GTGGCTCTTT AACAAGCTGG TGACGGAGGC CGATTCTGGC 
CTGTGCTCAC ACTACTGGGG CGCCATCCTT CGCCAGCAGC TCAGTCATAT TGAGGCCTGG 
GCTGAGAAAC AAGGCTTGGA GCTGGCTGCA GACTGCCACC TCAGCCGCAT AGTCCAGGCG 
ACGACCCTCC TTACTATGGA CAAGTACTCC ATGCAAGATG TCCAGAACAT CAACAACACC 
TGCTTCAAGC TCAACTCTCT TCAGCTCAAC GCTCTGCTGA GCAACTACCA CTGCGCTCCT 
GACGAACCCT ACATTCCTCC TGAGCTGATT GATCACGTGG TGGCCGTGGC CGAAAACACT 
GCAGACGAAC TGGCCCGAAG CGACGGGCGG GAGGTTCAGC TGGAAGAGGA CCCCGACCTT 
CAGCTGCCTT TCTTGCTGCC TGAGGACGGC TACTCGTGCG ATGTGGTGCG CAACATTCCC 
AACGGATTCC AAGAGTTTCT AGAGCCACTT TGCCAGAGAG GTTTCTGTAG ATTGACTCCT 
CATCCACGCT CGCCGGGAAC TTGGACCATT TATTTTGAAG GAGCCAACTG TGACAGTCAC 
TTCTCAGCCG ACACCTCAGA ACTGCCAATG CGGAAGGAAC CGGAAATTGT GACCGTGACT 
TTGAAGAAAC ATAATGGAAT GGGCCTCAGT ATTGTGGCGG CCAAGGGTGC CGGGCAGGAC 
AAACTTGGTA TTTACATCAA ATCTGTTGTC AAAGGAGGTG CAGCAGACAT GGATGGGCGT 
CTGGCTGCTG GAGACCAGTT GCTTAGCGTG GATGGACGTA GTCTTGTCGG CCTCTCGCAG 
GAGAGAGCGG CAGAATTGAT GACACGAACT GGGTCTGTTG TAACGCTTGA GGTGGCCAAA 
CAAGGAGCCA TTTATCACGG TCTGGCCACA CTTCTTAACC AGCCATCCCC CATGATGCCT 
CGCGCGTCAG ACCGTGGACG TGGCGACAAA GGAAAGCTGA GACCAAAGAG TGAGGGCTTT 
GAGCTCTACA ACAACTCTGT ACAGAACGGC TCACCTGAGA GCCCACAGGG CACGTGGGAC 
ACCTATCCTG AACCAAAGAA AGAGGACAGG TTGTTGAAGA ACCGAGCAGA CCATCGATCC 
AGCCCAAATG TGGCCAATCA GGCTCAGAGT CCAGGCGGGA AGCCTGTTTA TCCGGGAGCT 
CCAGGAAAGA TCACTTCGGT CTCGACGGGT AACCTCTGCC CTGATGAGGA GCCCTCTCCA 
CCCCGTCCAG AGGCCTATCC CATCCCCACT CAGACTTACC CTAGGGAATA CTTCACCTTC 
CCAGCCTCCA AATCTCAGGA CAGGATGGGC CCTGGCCAGC CCTGGCAAAA CAACGAACAA 
GAGCCTCTTC CCCCTATGGA TAACCACTCC AACAACAGCA TGGTTATGCA GCGGGTGGCG 
CGATCTCAGG AGGAGCTGTG TGACATGCCG GGCGGGTACC CAGGTGAACG TCTGCGCCAG 
GAAGATCTAA TGCTCCTCCA GCAGCAGCAG CAGCGTGAAG CTGAGTACCA GCGAGGTGTT 
GGAGAACACT GGAACCACCA GGTGTCTTCC TCAGTGGAGT CCAGCACCTC CAGCCAAGAG 
CACCTCAACT TCTCCTCATC CTCTGGCTCC AGGACCCAGG GCAACCACAA GAGTGGCCCA 
GGCCGCTGGA AGACCCCACT TGCACCTCAT TCCGGCCCTG TCTCCCAACC CTCCCGTTCA 
GACCTTCCTC CACCTCCACC TCCTCCACCA GCACACTACG ATTTTGAGCC GCAAACCGAC 
CTGCCGCTTC CACCGCCACC TTCAGCTACA CAACAACAGC AGCAGGCTGC AGCCGACCGC 
AAGAAACGTG AAGACCAACA GCGCTGGTAT GAGAAGGAAA AAGCTCGTCT TGAAGAGGAG 
CGAGAGAGGA AGAGACGAGA TCAGGAAAGG AAATTGGTGC AAATCCGAAA CCCTTCGGTT 
TCTGGTCCAG TTATGCACAA TAACCAGCAT GGCCCGGTGC CACCATCTCC ACAAATGCCT 
CCCATGACAC TTCCCCAATC ATACCTTCCT CCAGTCCAAC AACCTCCGCC ACCACAACCG 
GCTCGACCCG ATAAACTCTC CAGTCTTCCT CGCCAGCCTC CGCAAGACAC CGTAATCCGG 
GACCTCCTGC CGCAGCAGCA GCCGCGCACC ATCGAGAGAC GCGACCTGCA GTACATCACC 
ATCAGCAAGG AGGAGTTATC GGCCAGCGAC AGCCTGTCAC CCGACCCCTG GAAGAGGGAC 
GCCCGGGAAA AACTGGAGAA GCAGCAGCAG CTTCACATCG TTGACCTGCT GGACAAGGAG 
ATCCAGGAGC TGCAGGCTAA ACCCGAGCGC ACGGCCGAGG ACAACGACCG CCTGCGCAAA 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTGCAGGAGT CCAAGCAGAA CGAGGAGGAC 
GAGGAGGAGG AAGACGATGA AGACGTGGAC ACCACGCTCA TCATGCAGAG ACTGGAGGCC 
GAGAAGAGAG CACGGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGTC TGTACAGACC CCCTTTACCC CGGGACTACC AGCCCCCTTC TCCCTCAAGC 
GCACCCGCCA CTAACCACAC CACCAGCGCC CCCCCGCCTC CGCCTCAGAG AAACACCTCC 
TACCTCAAAA CCCAGGTCGC CTCCCCAGAC ACCATTTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGAGGAGGA GGATGAGGAA GATGCGGGCC TAACAGGATT TAATTCATAC 
ACTGGAAACA CAACAGGACT AGTATCAGGA GAGGTGTATA AAGACCCGCG AGACAAGTGG 
ACCAAAGGCC CGGATCAAGA GAACACAATA GGAGGAGCTC CAGAAAACCT GACCTTCAAG 
GAGCGCCAGA GACTCTTCTC GCAGGGGAAA GAGGTCTCCA ATAAAGTCAA AGCGTCCCGA 
AAGCTGATGG AGCTGGAGAA CGAACTCAAC ATAAAACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468692.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324367.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X17, mRNA.

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CloneID	ODa150788
Clone ID Related Accession (Same CDS sequence)	XM_021468692.1
Accession Version	XM_021468692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAG 
AGGAAACTGG ACTTGGATGA GAAACCACTG GTCGTGCAGC TGAACTGGAA TAAAGACGAC 
AGAGAGGGCC GCTTCGTTCT CAAGAACGAG AATGACATTA TGCCCAAGAG AACACTGACA 
AACGGTCCTG AAAAGGAGAA AGAGGGGGTG ATCCAGAACT TCAAAAGAAC ACTGTCCAAG 
AAGGAGAAGA AGAAAGAGAA GAAGAGGGAG AAGGAGGCTT TTTCCAGAAT CCCAGATGGA 
GACGATCATG TGTTGAACCG CGAGGATGGA GAGAACTCCA GATTAGCAGC TGAGGTTTAT 
AAAGACATGC CGGAGACCAG TTTCACAAGG ACCATTTCTA ACCCCGAGGT GGTGATGAAG 
AGAAGACGAC AGCAGAAGCT GGAGAAGAGG ATGCAGGAGT TTCGGAGTGA AGACGGTCGA 
CCCGACTCAG GAGGCACTCT GAGGATCTAC GCTGATAGTC TGAAGCCCAA CATTCCCTAC 
AAGACCATCC TGCTCTCAAC TACTGACATG GCTGACTTTG CTGTGGCCGA GGCTCTGGAG 
AAATATGGCC TGGAGAAGGA AAACCCTAGA GACTACTGCA TCGCCCAGGT GATTCATGCA 
AATGATAAAG CAGTGAAGGA GACCATATTA GATGACACAG ACTGTCCTCT GCAGATCCTC 
AGAGACTGGA CGAGTGATAA AGAGGCCTTA GTTTTCCAGC TGAAGAAGCG TCCTCCAGAT 
TACGCTCCAA GGAAAGGACG TAAGCCAGAC AACCGGATGG GGCCGCAGCA CGGATCCATT 
CCTCCTGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAG TCACTATGCC 
TACTACTACC GCCATCATGA AGATGGCTCC GACTCCAGGG ACAAACCCAA ACTCTACAGA 
CTACAGCACA GCGTTACTGA GGTCGGATCT GACCGGAGTG AAGATGGAAA CATCCAGCTG 
TTTGGGCCTG GGATTCTCCC TCATCACTGT GACCTTATGC ATGCGGACGG CTTGGTGACC 
ATAACCCCAG CCAATATGGA CGCAGAGACA TTTGTGGATG GACAGCGGAT CGCTGACACT 
ACAGTTCTGC GCAGTGGGGT GACCGTCCAG TTCGGAGCAA CTCACGTCTT CAAATTCGTA 
GACCCCAGTT ATGACCAGAG CATGTCCAAA AGAGACCCCG GGCCCATGAT GAAAGGCAGG 
CACAAATCAG GGAGTGTCCC AGAGACGACC TTTGACCTCC ACGGGGACAT TCACAGTGGC 
TCTGCTCTCC CTACAAGCAA GAGCTCAGGG AAGCTGGAGA TGGAGCGGTC AGGAAACAGC 
AGTGAAAGAG GCCTGGTGAA GCCCATGATT CGTGGAGAGC AGCAGGACTT TAGCAAGCAT 
GATGGAAGGT CTCAGGACGG CCCGGAGCTC ACTCTGCCCG CCAGCATCGA GTTCAGGGAA 
AACTCCGAGG ATGCCTTCCT GTCGGCCATC ATCAACTACA CCAACAGCTC GACAGTGCAC 
TTCAAGCTTT CTCCCACATA CGTGCTGTAC ATGACCTGCC GTTATGTCAT GTCCAGCCAA 
TACCGGCCCG ACATCAGTCC CTCCGAACGC ACACACAAAG TCATCGCCAT TGTCAACAAG 
ATGGTGAGCA TGATGGAAGG AGTCATCCAG GAGATTGCTG AAGGTGATCA GAAACAGAAG 
AACATCGCTG GAGCTCTGGC GTTTTGGATG GCGAACGCTT CTGAGCTGCT GAACTTCATC 
AAACAGGACA AGGATCTGAG TCGCATCACG CTGGATGCTC AGGATGTGCT CGCTCATCTT 
GTTCAGATGG CCTTCAAGTA CCTGGTGCAC TGTCTGCAGT CAGATCTCAA TAACTACATG 
CCAGCTTTCC TGGAAGATCC CGACGAGCAA AACCCACAGA GGCCTAAGAT AGAGGATGTT 
CTGCACACGC TGACGGGTGC CATGTCTCTG CTGCGCCGAT GCCGTGTGAA CGCGGCGCTC 
ACCATCCAAC TGTTTTCGCA GCTCTTCCAC TTCGTCAACA TGTGGCTCTT TAACAAGCTG 
GTGACGGAGG CCGATTCTGG CCTGTGCTCA CACTACTGGG GCGCCATCCT TCGCCAGCAG 
CTCAGTCATA TTGAGGCCTG GGCTGAGAAA CAAGGCTTGG AGCTGGCTGC AGACTGCCAC 
CTCAGCCGCA TAGTCCAGGC GACGACCCTC CTTACTATGG ACAAGTACTC CATGCAAGAT 
GTCCAGAACA TCAACAACAC CTGCTTCAAG CTCAACTCTC TTCAGCTCAA CGCTCTGCTG 
AGCAACTACC ACTGCGCTCC TGACGAACCC TACATTCCTC CTGAGCTGAT TGATCACGTG 
GTGGCCGTGG CCGAAAACAC TGCAGACGAA CTGGCCCGAA GCGACGGGCG GGAGGTTCAG 
CTGGAAGAGG ACCCCGACCT TCAGCTGCCT TTCTTGCTGC CTGAGGACGG CTACTCGTGC 
GATGTGGTGC GCAACATTCC CAACGGATTC CAAGAGTTTC TAGAGCCACT TTGCCAGAGA 
GGTTTCTGTA GATTGACTCC TCATCCACGC TCGCCGGGAA CTTGGACCAT TTATTTTGAA 
GGAGCCAACT GTGACAGTCA CTTCTCAGCC GACACCTCAG AACTGCCAAT GCGGAAGGAA 
CCGGAAATTG TGACCGTGAC TTTGAAGAAA CATAATGGAA TGGGCCTCAG TATTGTGGCG 
GCCAAGGGTG CCGGGCAGGA CAAACTTGGT ATTTACATCA AATCTGTTGT CAAAGGAGGT 
GCAGCAGACA TGGATGGGCG TCTGGCTGCT GGAGACCAGT TGCTTAGCGT GGATGGACGT 
AGTCTTGTCG GCCTCTCGCA GGAGAGAGCG GCAGAATTGA TGACACGAAC TGGGTCTGTT 
GTAACGCTTG AGGTGGCCAA ACAAGGAGCC ATTTATCACG GTCTGGCCAC ACTTCTTAAC 
CAGCCATCCC CCATGATGCC TCGCGCGTCA GACCGTGGAC GTGGCGACAA AGGAAAGCTG 
AGACCAAAGA GTGAGGGCTT TGAGCTCTAC AACAACTCTG TACAGAACGG CTCACCTGAG 
AGCCCACAGG GCACGTGGGA CACCTATCCT GAACCAAAGA AAGAGGACAG GTTGTTGAAG 
AACCGAGCAG ACCATCGATC CAGCCCAAAT GTGGCCAATC AGGCTCAGAG TCCAGGCGGG 
AAGCCTGTTT ATCCGGGAGC TCCAGGAAAG ATCACTTCGG TCTCGACGGG TAACCTCTGC 
CCTGATGAGG AGCCCTCTCC ACCCCGTCCA GAGGCCTATC CCATCCCCAC TCAGACTTAC 
CCTAGGGAAT ACTTCACCTT CCCAGCCTCC AAATCTCAGG ACAGGATGGG CCCTGGCCAG 
CCCTGGCAAA ACAACGAACA AGAGCCTCTT CCCCCTATGG ATAACCACTC CAACAACAGC 
ATGGTTATGC AGCGGGTGGC GCGATCTCAG GAGGAGCTGT GTGACATGCC GGGCGGGTAC 
CCAGGTGAAC GTCTGCGCCA GGAAGATCTA ATGCTCCTCC AGCAGCAGCA GCAGCGTGAA 
GCTGAGTACC AGCGAGGTGT TGGAGAACAC TGGAACCACC AGGTGTCTTC CTCAGTGGAG 
TCCAGCACCT CCAGCCAAGA GCACCTCAAC TTCTCCTCAT CCTCTGGCTC CAGGACCCAG 
GGCAACCACA AGAGTGGCCC AGGCCGCTGG AAGACCCCAC TTGCACCTCA TTCCGGCCCT 
GTCTCCCAAC CCTCCCGTTC AGACCTTCCT CCACCTCCAC CTCCTCCACC AGCACACTAC 
GATTTTGAGC CGCAAACCGA CCTGCCGCTT CCACCGCCAC CTTCAGCTAC ACAACAACAG 
CAGCAGGCTG CAGCCGACCG CAAGAAACGT GAAGACCAAC AGCGCTGGTA TGAGAAGGAA 
AAAGCTCGTC TTGAAGAGGA GCGAGAGAGG AAGAGACGAG ATCAGGAAAG GAAATTGGTG 
CAAATCCGAA ACCCTTCGGT TTCTGGTCCA GTTATGCACA ATAACCAGCA TGGCCCGGTG 
CCACCATCTC CACAAATGCC TCCCATGACA CTTCCCCAAT CATACCTTCC TCCAGTCCAA 
CAACCTCCGC CACCACAACC GGCTCGACCC GATAAACTCT CCAGTCTTCC TCGCCAGCCT 
CCGCAAGACA CCGTAATCCG GGACCTCCTG CCGCAGCAGC AGCCGCGCAC CATCGAGAGA 
CGCGACCTGC AGTACATCAC CATCAGCAAG GAGGAGTTAT CGGCCAGCGA CAGCCTGTCA 
CCCGACCCCT GGAAGAGGGA CGCCCGGGAA AAACTGGAGA AGCAGCAGCA GCTTCACATC 
GTTGACCTGC TGGACAAGGA GATCCAGGAG CTGCAGGCTA AACCCGAGCG CACGGCCGAG 
GACAACGACC GCCTGCGCAA ACTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTGCAGGAG 
TCCAAGCAGA ACGAGGAGGA CGAGGAGGAG GAAGACGATG AAGACGTGGA CACCACGCTC 
ATCATGCAGA GACTGGAGGC CGAGAAGAGA GCACGGCTGC AGGACGAGGA GCGACGACGG 
AAGCAGCAGC TGGAGGAAAT CCGCAAGCGC GAAGCAGAAG AGCGAGCCAA ACAGGAGGAG 
GAGAGACGCT GGAGAGAGGA GGAGAGAGCC AGGAGAGAGG CTGACGAGAA GAGGAGACAG 
GAGGAAGAGT ACTACACCCG CCTGGAGGCA GAGCGGCGCC GGCAGCACGA GGAAGCTGAG 
CGCAAGCTGC CGACGCCAGA TGAGACGGGT CTGTACAGAC CCCCTTTACC CCGGGACTAC 
CAGCCCCCTT CTCCCTCAAG CGCACCCGCC ACTAACCACA CCACCAGCGC CCCCCCGCCT 
CCGCCTCAGA GAAACACCTC CTACCTCAAA ACCCAGGTCG CCTCCCCAGA CACCATTTAC 
ACTGCCAAGT TTGTCTCGTA CAATGACGAG GAGGAGGAGG AGGATGAGGA AGATGCGGGC 
CTAACAGGTC AGGTTAAGCT CTCGGCCACC CGCAAGTCCT ACGGCGACCT TCCGCCTGCC 
CCAAAGCCCC AGCCGCCGCC AACCGCGCGC AAGCCCCGTC CCGTCTCAGA TGGCATCTTT 
CTGTCCAACT CATTCCAGCT GCCCGCTGGG CTCAATGCCA ACAGTACTGC TCTCAAGACA 
GGGCAGCCCC CTCTCCCCTT CACCAAACCC AGCTTCATCC CATCAGCCAA CTCCAAAGGA 
TTTAATTCAT ACACTGGAAA CACAACAGGA CTAGTATCAG GAGAGGTGTA TAAAGACCCG 
CGAGACAAGT GGACCAAAGG CAGCCCGGAT CAAGAGAACA CAATAGGAGG AGCTCCAGAA 
AACCTGACCT TCAAGGAGCG CCAGAGACTC TTCTCGCAGG GGAAAGAGGT CTCCAATAAA 
GTCAAAGCGT CCCGAAAGCT GATGGAGCTG GAGAACGAAC TCAACATAAA ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324367.1
CDS	280..6036
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468692.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468692.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAG 
AGGAAACTGG ACTTGGATGA GAAACCACTG GTCGTGCAGC TGAACTGGAA TAAAGACGAC 
AGAGAGGGCC GCTTCGTTCT CAAGAACGAG AATGACATTA TGCCCAAGAG AACACTGACA 
AACGGTCCTG AAAAGGAGAA AGAGGGGGTG ATCCAGAACT TCAAAAGAAC ACTGTCCAAG 
AAGGAGAAGA AGAAAGAGAA GAAGAGGGAG AAGGAGGCTT TTTCCAGAAT CCCAGATGGA 
GACGATCATG TGTTGAACCG CGAGGATGGA GAGAACTCCA GATTAGCAGC TGAGGTTTAT 
AAAGACATGC CGGAGACCAG TTTCACAAGG ACCATTTCTA ACCCCGAGGT GGTGATGAAG 
AGAAGACGAC AGCAGAAGCT GGAGAAGAGG ATGCAGGAGT TTCGGAGTGA AGACGGTCGA 
CCCGACTCAG GAGGCACTCT GAGGATCTAC GCTGATAGTC TGAAGCCCAA CATTCCCTAC 
AAGACCATCC TGCTCTCAAC TACTGACATG GCTGACTTTG CTGTGGCCGA GGCTCTGGAG 
AAATATGGCC TGGAGAAGGA AAACCCTAGA GACTACTGCA TCGCCCAGGT GATTCATGCA 
AATGATAAAG CAGTGAAGGA GACCATATTA GATGACACAG ACTGTCCTCT GCAGATCCTC 
AGAGACTGGA CGAGTGATAA AGAGGCCTTA GTTTTCCAGC TGAAGAAGCG TCCTCCAGAT 
TACGCTCCAA GGAAAGGACG TAAGCCAGAC AACCGGATGG GGCCGCAGCA CGGATCCATT 
CCTCCTGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAG TCACTATGCC 
TACTACTACC GCCATCATGA AGATGGCTCC GACTCCAGGG ACAAACCCAA ACTCTACAGA 
CTACAGCACA GCGTTACTGA GGTCGGATCT GACCGGAGTG AAGATGGAAA CATCCAGCTG 
TTTGGGCCTG GGATTCTCCC TCATCACTGT GACCTTATGC ATGCGGACGG CTTGGTGACC 
ATAACCCCAG CCAATATGGA CGCAGAGACA TTTGTGGATG GACAGCGGAT CGCTGACACT 
ACAGTTCTGC GCAGTGGGGT GACCGTCCAG TTCGGAGCAA CTCACGTCTT CAAATTCGTA 
GACCCCAGTT ATGACCAGAG CATGTCCAAA AGAGACCCCG GGCCCATGAT GAAAGGCAGG 
CACAAATCAG GGAGTGTCCC AGAGACGACC TTTGACCTCC ACGGGGACAT TCACAGTGGC 
TCTGCTCTCC CTACAAGCAA GAGCTCAGGG AAGCTGGAGA TGGAGCGGTC AGGAAACAGC 
AGTGAAAGAG GCCTGGTGAA GCCCATGATT CGTGGAGAGC AGCAGGACTT TAGCAAGCAT 
GATGGAAGGT CTCAGGACGG CCCGGAGCTC ACTCTGCCCG CCAGCATCGA GTTCAGGGAA 
AACTCCGAGG ATGCCTTCCT GTCGGCCATC ATCAACTACA CCAACAGCTC GACAGTGCAC 
TTCAAGCTTT CTCCCACATA CGTGCTGTAC ATGACCTGCC GTTATGTCAT GTCCAGCCAA 
TACCGGCCCG ACATCAGTCC CTCCGAACGC ACACACAAAG TCATCGCCAT TGTCAACAAG 
ATGGTGAGCA TGATGGAAGG AGTCATCCAG GAGATTGCTG AAGGTGATCA GAAACAGAAG 
AACATCGCTG GAGCTCTGGC GTTTTGGATG GCGAACGCTT CTGAGCTGCT GAACTTCATC 
AAACAGGACA AGGATCTGAG TCGCATCACG CTGGATGCTC AGGATGTGCT CGCTCATCTT 
GTTCAGATGG CCTTCAAGTA CCTGGTGCAC TGTCTGCAGT CAGATCTCAA TAACTACATG 
CCAGCTTTCC TGGAAGATCC CGACGAGCAA AACCCACAGA GGCCTAAGAT AGAGGATGTT 
CTGCACACGC TGACGGGTGC CATGTCTCTG CTGCGCCGAT GCCGTGTGAA CGCGGCGCTC 
ACCATCCAAC TGTTTTCGCA GCTCTTCCAC TTCGTCAACA TGTGGCTCTT TAACAAGCTG 
GTGACGGAGG CCGATTCTGG CCTGTGCTCA CACTACTGGG GCGCCATCCT TCGCCAGCAG 
CTCAGTCATA TTGAGGCCTG GGCTGAGAAA CAAGGCTTGG AGCTGGCTGC AGACTGCCAC 
CTCAGCCGCA TAGTCCAGGC GACGACCCTC CTTACTATGG ACAAGTACTC CATGCAAGAT 
GTCCAGAACA TCAACAACAC CTGCTTCAAG CTCAACTCTC TTCAGCTCAA CGCTCTGCTG 
AGCAACTACC ACTGCGCTCC TGACGAACCC TACATTCCTC CTGAGCTGAT TGATCACGTG 
GTGGCCGTGG CCGAAAACAC TGCAGACGAA CTGGCCCGAA GCGACGGGCG GGAGGTTCAG 
CTGGAAGAGG ACCCCGACCT TCAGCTGCCT TTCTTGCTGC CTGAGGACGG CTACTCGTGC 
GATGTGGTGC GCAACATTCC CAACGGATTC CAAGAGTTTC TAGAGCCACT TTGCCAGAGA 
GGTTTCTGTA GATTGACTCC TCATCCACGC TCGCCGGGAA CTTGGACCAT TTATTTTGAA 
GGAGCCAACT GTGACAGTCA CTTCTCAGCC GACACCTCAG AACTGCCAAT GCGGAAGGAA 
CCGGAAATTG TGACCGTGAC TTTGAAGAAA CATAATGGAA TGGGCCTCAG TATTGTGGCG 
GCCAAGGGTG CCGGGCAGGA CAAACTTGGT ATTTACATCA AATCTGTTGT CAAAGGAGGT 
GCAGCAGACA TGGATGGGCG TCTGGCTGCT GGAGACCAGT TGCTTAGCGT GGATGGACGT 
AGTCTTGTCG GCCTCTCGCA GGAGAGAGCG GCAGAATTGA TGACACGAAC TGGGTCTGTT 
GTAACGCTTG AGGTGGCCAA ACAAGGAGCC ATTTATCACG GTCTGGCCAC ACTTCTTAAC 
CAGCCATCCC CCATGATGCC TCGCGCGTCA GACCGTGGAC GTGGCGACAA AGGAAAGCTG 
AGACCAAAGA GTGAGGGCTT TGAGCTCTAC AACAACTCTG TACAGAACGG CTCACCTGAG 
AGCCCACAGG GCACGTGGGA CACCTATCCT GAACCAAAGA AAGAGGACAG GTTGTTGAAG 
AACCGAGCAG ACCATCGATC CAGCCCAAAT GTGGCCAATC AGGCTCAGAG TCCAGGCGGG 
AAGCCTGTTT ATCCGGGAGC TCCAGGAAAG ATCACTTCGG TCTCGACGGG TAACCTCTGC 
CCTGATGAGG AGCCCTCTCC ACCCCGTCCA GAGGCCTATC CCATCCCCAC TCAGACTTAC 
CCTAGGGAAT ACTTCACCTT CCCAGCCTCC AAATCTCAGG ACAGGATGGG CCCTGGCCAG 
CCCTGGCAAA ACAACGAACA AGAGCCTCTT CCCCCTATGG ATAACCACTC CAACAACAGC 
ATGGTTATGC AGCGGGTGGC GCGATCTCAG GAGGAGCTGT GTGACATGCC GGGCGGGTAC 
CCAGGTGAAC GTCTGCGCCA GGAAGATCTA ATGCTCCTCC AGCAGCAGCA GCAGCGTGAA 
GCTGAGTACC AGCGAGGTGT TGGAGAACAC TGGAACCACC AGGTGTCTTC CTCAGTGGAG 
TCCAGCACCT CCAGCCAAGA GCACCTCAAC TTCTCCTCAT CCTCTGGCTC CAGGACCCAG 
GGCAACCACA AGAGTGGCCC AGGCCGCTGG AAGACCCCAC TTGCACCTCA TTCCGGCCCT 
GTCTCCCAAC CCTCCCGTTC AGACCTTCCT CCACCTCCAC CTCCTCCACC AGCACACTAC 
GATTTTGAGC CGCAAACCGA CCTGCCGCTT CCACCGCCAC CTTCAGCTAC ACAACAACAG 
CAGCAGGCTG CAGCCGACCG CAAGAAACGT GAAGACCAAC AGCGCTGGTA TGAGAAGGAA 
AAAGCTCGTC TTGAAGAGGA GCGAGAGAGG AAGAGACGAG ATCAGGAAAG GAAATTGGTG 
CAAATCCGAA ACCCTTCGGT TTCTGGTCCA GTTATGCACA ATAACCAGCA TGGCCCGGTG 
CCACCATCTC CACAAATGCC TCCCATGACA CTTCCCCAAT CATACCTTCC TCCAGTCCAA 
CAACCTCCGC CACCACAACC GGCTCGACCC GATAAACTCT CCAGTCTTCC TCGCCAGCCT 
CCGCAAGACA CCGTAATCCG GGACCTCCTG CCGCAGCAGC AGCCGCGCAC CATCGAGAGA 
CGCGACCTGC AGTACATCAC CATCAGCAAG GAGGAGTTAT CGGCCAGCGA CAGCCTGTCA 
CCCGACCCCT GGAAGAGGGA CGCCCGGGAA AAACTGGAGA AGCAGCAGCA GCTTCACATC 
GTTGACCTGC TGGACAAGGA GATCCAGGAG CTGCAGGCTA AACCCGAGCG CACGGCCGAG 
GACAACGACC GCCTGCGCAA ACTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTGCAGGAG 
TCCAAGCAGA ACGAGGAGGA CGAGGAGGAG GAAGACGATG AAGACGTGGA CACCACGCTC 
ATCATGCAGA GACTGGAGGC CGAGAAGAGA GCACGGCTGC AGGACGAGGA GCGACGACGG 
AAGCAGCAGC TGGAGGAAAT CCGCAAGCGC GAAGCAGAAG AGCGAGCCAA ACAGGAGGAG 
GAGAGACGCT GGAGAGAGGA GGAGAGAGCC AGGAGAGAGG CTGACGAGAA GAGGAGACAG 
GAGGAAGAGT ACTACACCCG CCTGGAGGCA GAGCGGCGCC GGCAGCACGA GGAAGCTGAG 
CGCAAGCTGC CGACGCCAGA TGAGACGGGT CTGTACAGAC CCCCTTTACC CCGGGACTAC 
CAGCCCCCTT CTCCCTCAAG CGCACCCGCC ACTAACCACA CCACCAGCGC CCCCCCGCCT 
CCGCCTCAGA GAAACACCTC CTACCTCAAA ACCCAGGTCG CCTCCCCAGA CACCATTTAC 
ACTGCCAAGT TTGTCTCGTA CAATGACGAG GAGGAGGAGG AGGATGAGGA AGATGCGGGC 
CTAACAGGTC AGGTTAAGCT CTCGGCCACC CGCAAGTCCT ACGGCGACCT TCCGCCTGCC 
CCAAAGCCCC AGCCGCCGCC AACCGCGCGC AAGCCCCGTC CCGTCTCAGA TGGCATCTTT 
CTGTCCAACT CATTCCAGCT GCCCGCTGGG CTCAATGCCA ACAGTACTGC TCTCAAGACA 
GGGCAGCCCC CTCTCCCCTT CACCAAACCC AGCTTCATCC CATCAGCCAA CTCCAAAGGA 
TTTAATTCAT ACACTGGAAA CACAACAGGA CTAGTATCAG GAGAGGTGTA TAAAGACCCG 
CGAGACAAGT GGACCAAAGG CAGCCCGGAT CAAGAGAACA CAATAGGAGG AGCTCCAGAA 
AACCTGACCT TCAAGGAGCG CCAGAGACTC TTCTCGCAGG GGAAAGAGGT CTCCAATAAA 
GTCAAAGCGT CCCGAAAGCT GATGGAGCTG GAGAACGAAC TCAACATAAA ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468693.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324368.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X18, mRNA.

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CloneID	ODa150789
Clone ID Related Accession (Same CDS sequence)	XM_021468693.1
Accession Version	XM_021468693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGAAACAGA AGAACATCGC TGGAGCTCTG 
GCGTTTTGGA TGGCGAACGC TTCTGAGCTG CTGAACTTCA TCAAACAGGA CAAGGATCTG 
AGTCGCATCA CGCTGGATGC TCAGGATGTG CTCGCTCATC TTGTTCAGAT GGCCTTCAAG 
TACCTGGTGC ACTGTCTGCA GTCAGATCTC AATAACTACA TGCCAGCTTT CCTGGAAGAT 
CCCGACGAGC AAAACCCACA GAGGCCTAAG ATAGAGGATG TTCTGCACAC GCTGACGGGT 
GCCATGTCTC TGCTGCGCCG ATGCCGTGTG AACGCGGCGC TCACCATCCA ACTGTTTTCG 
CAGCTCTTCC ACTTCGTCAA CATGTGGCTC TTTAACAAGC TGGTGACGGA GGCCGATTCT 
GGCCTGTGCT CACACTACTG GGGCGCCATC CTTCGCCAGC AGCTCAGTCA TATTGAGGCC 
TGGGCTGAGA AACAAGGCTT GGAGCTGGCT GCAGACTGCC ACCTCAGCCG CATAGTCCAG 
GCGACGACCC TCCTTACTAT GGACAAGTAC TCCATGCAAG ATGTCCAGAA CATCAACAAC 
ACCTGCTTCA AGCTCAACTC TCTTCAGCTC AACGCTCTGC TGAGCAACTA CCACTGCGCT 
CCTGACGAAC CCTACATTCC TCCTGAGCTG ATTGATCACG TGGTGGCCGT GGCCGAAAAC 
ACTGCAGACG AACTGGCCCG AAGCGACGGG CGGGAGGTTC AGCTGGAAGA GGACCCCGAC 
CTTCAGCTGC CTTTCTTGCT GCCTGAGGAC GGCTACTCGT GCGATGTGGT GCGCAACATT 
CCCAACGGAT TCCAAGAGTT TCTAGAGCCA CTTTGCCAGA GAGGTTTCTG TAGATTGACT 
CCTCATCCAC GCTCGCCGGG AACTTGGACC ATTTATTTTG AAGGAGCCAA CTGTGACAGT 
CACTTCTCAG CCGACACCTC AGAACTGCCA ATGCGGAAGG AACCGGAAAT TGTGACCGTG 
ACTTTGAAGA AACATAATGG AATGGGCCTC AGTATTGTGG CGGCCAAGGG TGCCGGGCAG 
GACAAACTTG GTATTTACAT CAAATCTGTT GTCAAAGGAG GTGCAGCAGA CATGGATGGG 
CGTCTGGCTG CTGGAGACCA GTTGCTTAGC GTGGATGGAC GTAGTCTTGT CGGCCTCTCG 
CAGGAGAGAG CGGCAGAATT GATGACACGA ACTGGGTCTG TTGTAACGCT TGAGGTGGCC 
AAACAAGGAG CCATTTATCA CGGTCTGGCC ACACTTCTTA ACCAGCCATC CCCCATGATG 
CCTCGCGCGT CAGACCGTGG ACGTGGCGAC AAAGGAAAGC TGAGACCAAA GAGTGAGGGC 
TTTGAGCTCT ACAACAACTC TGTACAGAAC GGCTCACCTG AGAGCCCACA GGGCACGTGG 
GACACCTATC CTGAACCAAA GAAAGAGGAC AGGTTGTTGA AGAACCGAGC AGACCATCGA 
TCCAGCCCAA ATGTGGCCAA TCAGGCTCAG AGTCCAGGCG GGAAGCCTGT TTATCCGGGA 
GCTCCAGGAA AGATCACTTC GGTCTCGACG GGTAACCTCT GCCCTGATGA GGAGCCCTCT 
CCACCCCGTC CAGAGGCCTA TCCCATCCCC ACTCAGACTT ACCCTAGGGA ATACTTCACC 
TTCCCAGCCT CCAAATCTCA GGACAGGATG GGCCCTGGCC AGCCCTGGCA AAACAACGAA 
CAAGAGCCTC TTCCCCCTAT GGATAACCAC TCCAACAACA GCATGGTTAT GCAGCGGGTG 
GCGCGATCTC AGGAGGAGCT GTGTGACATG CCGGGCGGGT ACCCAGGTGA ACGTCTGCGC 
CAGGAAGATC TAATGCTCCT CCAGCAGCAG CAGCAGCGTG AAGCTGAGTA CCAGCGAGGT 
GTTGGAGAAC ACTGGAACCA CCAGGTGTCT TCCTCAGTGG AGTCCAGCAC CTCCAGCCAA 
GAGCACCTCA ACTTCTCCTC ATCCTCTGGC TCCAGGACCC AGGGCAACCA CAAGAGTGGC 
CCAGGCCGCT GGAAGACCCC ACTTGCACCT CATTCCGGCC CTGTCTCCCA ACCCTCCCGT 
TCAGACCTTC CTCCACCTCC ACCTCCTCCA CCAGCACACT ACGATTTTGA GCCGCAAACC 
GACCTGCCGC TTCCACCGCC ACCTTCAGCT ACACAACAAC AGCAGCAGGC TGCAGCCGAC 
CGCAAGAAAC GTGAAGACCA ACAGCGCTGG TATGAGAAGG AAAAAGCTCG TCTTGAAGAG 
GAGCGAGAGA GGAAGAGACG AGATCAGGAA AGGAAATTGG TGCAAATCCG AAACCCTTCG 
GTTTCTGGTC CAGTTATGCA CAATAACCAG CATGGCCCGG TGCCACCATC TCCACAAATG 
CCTCCCATGA CACTTCCCCA ATCATACCTT CCTCCAGTCC AACAACCTCC GCCACCACAA 
CCGGCTCGAC CCGATAAACT CTCCAGTCTT CCTCGCCAGC CTCCGCAAGA CACCGTAATC 
CGGGACCTCC TGCCGCAGCA GCAGCCGCGC ACCATCGAGA GACGCGACCT GCAGTACATC 
ACCATCAGCA AGGAGGAGTT ATCGGCCAGC GACAGCCTGT CACCCGACCC CTGGAAGAGG 
GACGCCCGGG AAAAACTGGA GAAGCAGCAG CAGCTTCACA TCGTTGACCT GCTGGACAAG 
GAGATCCAGG AGCTGCAGGC TAAACCCGAG CGCACGGCCG AGGACAACGA CCGCCTGCGC 
AAACTCATGC TGGAGTGGCA GTTCCAGAAG AGACTGCAGG AGTCCAAGCA GAACGAGGAG 
GACGAGGAGG AGGAAGACGA TGAAGACGTG GACACCACGC TCATCATGCA GAGACTGGAG 
GCCGAGAAGA GAGCACGGCA GACTGCTGTT CCAGCAATCT CTGTACTAGA CTTGCTGCAG 
GACGAGGAGC GACGACGGAA GCAGCAGCTG GAGGAAATCC GCAAGCGCGA AGCAGAAGAG 
CGAGCCAAAC AGGAGGAGGA GAGACGCTGG AGAGAGGAGG AGAGAGCCAG GAGAGAGGCT 
GACGAGAAGA GGAGACAGGA GGAAGAGTAC TACACCCGCC TGGAGGCAGA GCGGCGCCGG 
CAGCACGAGG AAGCTGAGCG CAAGCTGCCG ACGCCAGATG AGACGGGTCT GTACAGACCC 
CCTTTACCCC GGGACTACCA GCCCCCTTCT CCCTCAAGCG CACCCGCCAC TAACCACACC 
ACCAGCGCCC CCCCGCCTCC GCCTCAGAGA AACACCTCCT ACCTCAAAAC CCAGGTCGCC 
TCCCCAGACA CCATTTACAC TGCCAAGTTT GTCTCGTACA ATGACGAGGA GGAGGAGGAG 
GATGAGGAAG ATGCGGGCCT AACAGGATTT AATTCATACA CTGGAAACAC AACAGGACTA 
GTATCAGGAG AGGTGTATAA AGACCCGCGA GACAAGTGGA CCAAAGGCCC GGATCAAGAG 
AACACAATAG GAGGAGCTCC AGAAAACCTG ACCTTCAAGG AGCGCCAGAG ACTCTTCTCG 
CAGGGGAAAG AGGTCTCCAA TAAAGTCAAA GCGTCCCGAA AGCTGATGGA GCTGGAGAAC 
GAACTCAACA TAAAACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324368.1
CDS	281..5821
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468693.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468693.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGAAACAGA AGAACATCGC TGGAGCTCTG 
GCGTTTTGGA TGGCGAACGC TTCTGAGCTG CTGAACTTCA TCAAACAGGA CAAGGATCTG 
AGTCGCATCA CGCTGGATGC TCAGGATGTG CTCGCTCATC TTGTTCAGAT GGCCTTCAAG 
TACCTGGTGC ACTGTCTGCA GTCAGATCTC AATAACTACA TGCCAGCTTT CCTGGAAGAT 
CCCGACGAGC AAAACCCACA GAGGCCTAAG ATAGAGGATG TTCTGCACAC GCTGACGGGT 
GCCATGTCTC TGCTGCGCCG ATGCCGTGTG AACGCGGCGC TCACCATCCA ACTGTTTTCG 
CAGCTCTTCC ACTTCGTCAA CATGTGGCTC TTTAACAAGC TGGTGACGGA GGCCGATTCT 
GGCCTGTGCT CACACTACTG GGGCGCCATC CTTCGCCAGC AGCTCAGTCA TATTGAGGCC 
TGGGCTGAGA AACAAGGCTT GGAGCTGGCT GCAGACTGCC ACCTCAGCCG CATAGTCCAG 
GCGACGACCC TCCTTACTAT GGACAAGTAC TCCATGCAAG ATGTCCAGAA CATCAACAAC 
ACCTGCTTCA AGCTCAACTC TCTTCAGCTC AACGCTCTGC TGAGCAACTA CCACTGCGCT 
CCTGACGAAC CCTACATTCC TCCTGAGCTG ATTGATCACG TGGTGGCCGT GGCCGAAAAC 
ACTGCAGACG AACTGGCCCG AAGCGACGGG CGGGAGGTTC AGCTGGAAGA GGACCCCGAC 
CTTCAGCTGC CTTTCTTGCT GCCTGAGGAC GGCTACTCGT GCGATGTGGT GCGCAACATT 
CCCAACGGAT TCCAAGAGTT TCTAGAGCCA CTTTGCCAGA GAGGTTTCTG TAGATTGACT 
CCTCATCCAC GCTCGCCGGG AACTTGGACC ATTTATTTTG AAGGAGCCAA CTGTGACAGT 
CACTTCTCAG CCGACACCTC AGAACTGCCA ATGCGGAAGG AACCGGAAAT TGTGACCGTG 
ACTTTGAAGA AACATAATGG AATGGGCCTC AGTATTGTGG CGGCCAAGGG TGCCGGGCAG 
GACAAACTTG GTATTTACAT CAAATCTGTT GTCAAAGGAG GTGCAGCAGA CATGGATGGG 
CGTCTGGCTG CTGGAGACCA GTTGCTTAGC GTGGATGGAC GTAGTCTTGT CGGCCTCTCG 
CAGGAGAGAG CGGCAGAATT GATGACACGA ACTGGGTCTG TTGTAACGCT TGAGGTGGCC 
AAACAAGGAG CCATTTATCA CGGTCTGGCC ACACTTCTTA ACCAGCCATC CCCCATGATG 
CCTCGCGCGT CAGACCGTGG ACGTGGCGAC AAAGGAAAGC TGAGACCAAA GAGTGAGGGC 
TTTGAGCTCT ACAACAACTC TGTACAGAAC GGCTCACCTG AGAGCCCACA GGGCACGTGG 
GACACCTATC CTGAACCAAA GAAAGAGGAC AGGTTGTTGA AGAACCGAGC AGACCATCGA 
TCCAGCCCAA ATGTGGCCAA TCAGGCTCAG AGTCCAGGCG GGAAGCCTGT TTATCCGGGA 
GCTCCAGGAA AGATCACTTC GGTCTCGACG GGTAACCTCT GCCCTGATGA GGAGCCCTCT 
CCACCCCGTC CAGAGGCCTA TCCCATCCCC ACTCAGACTT ACCCTAGGGA ATACTTCACC 
TTCCCAGCCT CCAAATCTCA GGACAGGATG GGCCCTGGCC AGCCCTGGCA AAACAACGAA 
CAAGAGCCTC TTCCCCCTAT GGATAACCAC TCCAACAACA GCATGGTTAT GCAGCGGGTG 
GCGCGATCTC AGGAGGAGCT GTGTGACATG CCGGGCGGGT ACCCAGGTGA ACGTCTGCGC 
CAGGAAGATC TAATGCTCCT CCAGCAGCAG CAGCAGCGTG AAGCTGAGTA CCAGCGAGGT 
GTTGGAGAAC ACTGGAACCA CCAGGTGTCT TCCTCAGTGG AGTCCAGCAC CTCCAGCCAA 
GAGCACCTCA ACTTCTCCTC ATCCTCTGGC TCCAGGACCC AGGGCAACCA CAAGAGTGGC 
CCAGGCCGCT GGAAGACCCC ACTTGCACCT CATTCCGGCC CTGTCTCCCA ACCCTCCCGT 
TCAGACCTTC CTCCACCTCC ACCTCCTCCA CCAGCACACT ACGATTTTGA GCCGCAAACC 
GACCTGCCGC TTCCACCGCC ACCTTCAGCT ACACAACAAC AGCAGCAGGC TGCAGCCGAC 
CGCAAGAAAC GTGAAGACCA ACAGCGCTGG TATGAGAAGG AAAAAGCTCG TCTTGAAGAG 
GAGCGAGAGA GGAAGAGACG AGATCAGGAA AGGAAATTGG TGCAAATCCG AAACCCTTCG 
GTTTCTGGTC CAGTTATGCA CAATAACCAG CATGGCCCGG TGCCACCATC TCCACAAATG 
CCTCCCATGA CACTTCCCCA ATCATACCTT CCTCCAGTCC AACAACCTCC GCCACCACAA 
CCGGCTCGAC CCGATAAACT CTCCAGTCTT CCTCGCCAGC CTCCGCAAGA CACCGTAATC 
CGGGACCTCC TGCCGCAGCA GCAGCCGCGC ACCATCGAGA GACGCGACCT GCAGTACATC 
ACCATCAGCA AGGAGGAGTT ATCGGCCAGC GACAGCCTGT CACCCGACCC CTGGAAGAGG 
GACGCCCGGG AAAAACTGGA GAAGCAGCAG CAGCTTCACA TCGTTGACCT GCTGGACAAG 
GAGATCCAGG AGCTGCAGGC TAAACCCGAG CGCACGGCCG AGGACAACGA CCGCCTGCGC 
AAACTCATGC TGGAGTGGCA GTTCCAGAAG AGACTGCAGG AGTCCAAGCA GAACGAGGAG 
GACGAGGAGG AGGAAGACGA TGAAGACGTG GACACCACGC TCATCATGCA GAGACTGGAG 
GCCGAGAAGA GAGCACGGCA GACTGCTGTT CCAGCAATCT CTGTACTAGA CTTGCTGCAG 
GACGAGGAGC GACGACGGAA GCAGCAGCTG GAGGAAATCC GCAAGCGCGA AGCAGAAGAG 
CGAGCCAAAC AGGAGGAGGA GAGACGCTGG AGAGAGGAGG AGAGAGCCAG GAGAGAGGCT 
GACGAGAAGA GGAGACAGGA GGAAGAGTAC TACACCCGCC TGGAGGCAGA GCGGCGCCGG 
CAGCACGAGG AAGCTGAGCG CAAGCTGCCG ACGCCAGATG AGACGGGTCT GTACAGACCC 
CCTTTACCCC GGGACTACCA GCCCCCTTCT CCCTCAAGCG CACCCGCCAC TAACCACACC 
ACCAGCGCCC CCCCGCCTCC GCCTCAGAGA AACACCTCCT ACCTCAAAAC CCAGGTCGCC 
TCCCCAGACA CCATTTACAC TGCCAAGTTT GTCTCGTACA ATGACGAGGA GGAGGAGGAG 
GATGAGGAAG ATGCGGGCCT AACAGGATTT AATTCATACA CTGGAAACAC AACAGGACTA 
GTATCAGGAG AGGTGTATAA AGACCCGCGA GACAAGTGGA CCAAAGGCCC GGATCAAGAG 
AACACAATAG GAGGAGCTCC AGAAAACCTG ACCTTCAAGG AGCGCCAGAG ACTCTTCTCG 
CAGGGGAAAG AGGTCTCCAA TAAAGTCAAA GCGTCCCGAA AGCTGATGGA GCTGGAGAAC 
GAACTCAACA TAAAACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468694.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324369.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X19, mRNA.

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CloneID	ODa150790
Clone ID Related Accession (Same CDS sequence)	XM_021468694.1
Accession Version	XM_021468694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAG 
AGGAAACTGG ACTTGGATGA GAAACCACTG GTCGTGCAGC TGAACTGGAA TAAAGACGAC 
AGAGAGGGCC GCTTCGTTCT CAAGAACGAG AATGACATTA TGCCCAAGAG AACACTGACA 
AACGGTCCTG AAAAGGAGAA AGAGGGGGTG ATCCAGAACT TCAAAAGAAC ACTGTCCAAG 
AAGGAGAAGA AGAAAGAGAA GAAGAGGGAG AAGGAGGCTT TTTCCAGAAT CCCAGATGGA 
GACGATCATG TGTTGAACCG CGAGGATGGA GAGAACTCCA GATTAGCAGC TGAGGTTTAT 
AAAGACATGC CGGAGACCAG TTTCACAAGG ACCATTTCTA ACCCCGAGGT GGTGATGAAG 
AGAAGACGAC AGCAGAAGCT GGAGAAGAGG ATGCAGGAGT TTCGGAGTGA AGACGGTCGA 
CCCGACTCAG GAGGCACTCT GAGGATCTAC GCTGATAGTC TGAAGCCCAA CATTCCCTAC 
AAGACCATCC TGCTCTCAAC TACTGACATG GCTGACTTTG CTGTGGCCGA GGCTCTGGAG 
AAATATGGCC TGGAGAAGGA AAACCCTAGA GACTACTGCA TCGCCCAGGT GATTCATGCA 
AATGATAAAG CAGTGAAGGA GACCATATTA GATGACACAG ACTGTCCTCT GCAGATCCTC 
AGAGACTGGA CGAGTGATAA AGAGGCCTTA GTTTTCCAGC TGAAGAAGCG TCCTCCAGAT 
TACGCTCCAA GGAAAGGACG TAAGCCAGAC AACCGGATGG GGCCGCAGCA CGGATCCATT 
CCTCCTGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG ATGGCTCCGA CTCCAGGGAC 
AAACCCAAAC TCTACAGACT ACAGCACAGC GTTACTGAGG TCGGATCTGA CCGGAGTGAA 
GATGGAAACA TCCAGCTGTT TGGGCCTGGG ATTCTCCCTC ATCACTGTGA CCTTATGCAT 
GCGGACGGCT TGGTGACCAT AACCCCAGCC AATATGGACG CAGAGACATT TGTGGATGGA 
CAGCGGATCG CTGACACTAC AGTTCTGCGC AGTGGGGTGA CCGTCCAGTT CGGAGCAACT 
CACGTCTTCA AATTCGTAGA CCCCAGTTAT GACCAGAGCA TGTCCAAAAG AGACCCCGGG 
CCCATGATGA AAGGCAGGCA CAAATCAGGG AGTGTCCCAG AGACGACCTT TGACCTCCAC 
GGGGACATTC ACAGTGGCTC TGCTCTCCCT ACAAGCAAGA GCTCAGGGAA GCTGGAGATG 
GAGCGGTCAG GAAACAGCAG TGAAAGAGGC CTGGTGAAGC CCATGATTCG TGGAGAGCAG 
CAGGACTTTA GCAAGCATGA TGGAAGGTCT CAGGACGGCC CGGAGCTCAC TCTGCCCGCC 
AGCATCGAGT TCAGGGAAAA CTCCGAGGAT GCCTTCCTGT CGGCCATCAT CAACTACACC 
AACAGCTCGA CAGTGCACTT CAAGCTTTCT CCCACATACG TGCTGTACAT GACCTGCCGT 
TATGTCATGT CCAGCCAATA CCGGCCCGAC ATCAGTCCCT CCGAACGCAC ACACAAAGTC 
ATCGCCATTG TCAACAAGAT GGTGAGCATG ATGGAAGGAG TCATCCAGAA ACAGAAGAAC 
ATCGCTGGAG CTCTGGCGTT TTGGATGGCG AACGCTTCTG AGCTGCTGAA CTTCATCAAA 
CAGGACAAGG ATCTGAGTCG CATCACGCTG GATGCTCAGG ATGTGCTCGC TCATCTTGTT 
CAGATGGCCT TCAAGTACCT GGTGCACTGT CTGCAGTCAG ATCTCAATAA CTACATGCCA 
GCTTTCCTGG AAGATCCCGA CGAGCAAAAC CCACAGAGGC CTAAGATAGA GGATGTTCTG 
CACACGCTGA CGGGTGCCAT GTCTCTGCTG CGCCGATGCC GTGTGAACGC GGCGCTCACC 
ATCCAACTGT TTTCGCAGCT CTTCCACTTC GTCAACATGT GGCTCTTTAA CAAGCTGGTG 
ACGGAGGCCG ATTCTGGCCT GTGCTCACAC TACTGGGGCG CCATCCTTCG CCAGCAGCTC 
AGTCATATTG AGGCCTGGGC TGAGAAACAA GGCTTGGAGC TGGCTGCAGA CTGCCACCTC 
AGCCGCATAG TCCAGGCGAC GACCCTCCTT ACTATGGACA AGTACTCCAT GCAAGATGTC 
CAGAACATCA ACAACACCTG CTTCAAGCTC AACTCTCTTC AGCTCAACGC TCTGCTGAGC 
AACTACCACT GCGCTCCTGA CGAACCCTAC ATTCCTCCTG AGCTGATTGA TCACGTGGTG 
GCCGTGGCCG AAAACACTGC AGACGAACTG GCCCGAAGCG ACGGGCGGGA GGTTCAGCTG 
GAAGAGGACC CCGACCTTCA GCTGCCTTTC TTGCTGCCTG AGGACGGCTA CTCGTGCGAT 
GTGGTGCGCA ACATTCCCAA CGGATTCCAA GAGTTTCTAG AGCCACTTTG CCAGAGAGGT 
TTCTGTAGAT TGACTCCTCA TCCACGCTCG CCGGGAACTT GGACCATTTA TTTTGAAGGA 
GCCAACTGTG ACAGTCACTT CTCAGCCGAC ACCTCAGAAC TGCCAATGCG GAAGGAACCG 
GAAATTGTGA CCGTGACTTT GAAGAAACAT AATGGAATGG GCCTCAGTAT TGTGGCGGCC 
AAGGGTGCCG GGCAGGACAA ACTTGGTATT TACATCAAAT CTGTTGTCAA AGGAGGTGCA 
GCAGACATGG ATGGGCGTCT GGCTGCTGGA GACCAGTTGC TTAGCGTGGA TGGACGTAGT 
CTTGTCGGCC TCTCGCAGGA GAGAGCGGCA GAATTGATGA CACGAACTGG GTCTGTTGTA 
ACGCTTGAGG TGGCCAAACA AGGAGCCATT TATCACGGTC TGGCCACACT TCTTAACCAG 
CCATCCCCCA TGATGCCTCG CGCGTCAGAC CGTGGACGTG GCGACAAAGG AAAGCTGAGA 
CCAAAGAGTG AGGGCTTTGA GCTCTACAAC AACTCTGTAC AGAACGGCTC ACCTGAGAGC 
CCACAGGGCA CGTGGGACAC CTATCCTGAA CCAAAGAAAG AGGACAGGTT GTTGAAGAAC 
CGAGCAGACC ATCGATCCAG CCCAAATGTG GCCAATCAGG CTCAGAGTCC AGGCGGGAAG 
CCTGTTTATC CGGGAGCTCC AGGAAAGATC ACTTCGGTCT CGACGGGTAA CCTCTGCCCT 
GATGAGGAGC CCTCTCCACC CCGTCCAGAG GCCTATCCCA TCCCCACTCA GACTTACCCT 
AGGGAATACT TCACCTTCCC AGCCTCCAAA TCTCAGGACA GGATGGGCCC TGGCCAGCCC 
TGGCAAAACA ACGAACAAGA GCCTCTTCCC CCTATGGATA ACCACTCCAA CAACAGCATG 
GTTATGCAGC GGGTGGCGCG ATCTCAGGAG GAGCTGTGTG ACATGCCGGG CGGGTACCCA 
GGTGAACGTC TGCGCCAGGA AGATCTAATG CTCCTCCAGC AGCAGCAGCA GCGTGAAGCT 
GAGTACCAGC GAGGTGTTGG AGAACACTGG AACCACCAGG TGTCTTCCTC AGTGGAGTCC 
AGCACCTCCA GCCAAGAGCA CCTCAACTTC TCCTCATCCT CTGGCTCCAG GACCCAGGGC 
AACCACAAGA GTGGCCCAGG CCGCTGGAAG ACCCCACTTG CACCTCATTC CGGCCCTGTC 
TCCCAACCCT CCCGTTCAGA CCTTCCTCCA CCTCCACCTC CTCCACCAGC ACACTACGAT 
TTTGAGCCGC AAACCGACCT GCCGCTTCCA CCGCCACCTT CAGCTACACA ACAACAGCAG 
CAGGCTGCAG CCGACCGCAA GAAACGTGAA GACCAACAGC GCTGGTATGA GAAGGAAAAA 
GCTCGTCTTG AAGAGGAGCG AGAGAGGAAG AGACGAGATC AGGAAAGGAA ATTGGTGCAA 
ATCCGAAACC CTTCGGTTTC TGGTCCAGTT ATGCACAATA ACCAGCATGG CCCGGTGCCA 
CCATCTCCAC AAATGCCTCC CATGACACTT CCCCAATCAT ACCTTCCTCC AGTCCAACAA 
CCTCCGCCAC CACAACCGGC TCGACCCGAT AAACTCTCCA GTCTTCCTCG CCAGCCTCCG 
CAAGACACCG TAATCCGGGA CCTCCTGCCG CAGCAGCAGC CGCGCACCAT CGAGAGACGC 
GACCTGCAGT ACATCACCAT CAGCAAGGAG GAGTTATCGG CCAGCGACAG CCTGTCACCC 
GACCCCTGGA AGAGGGACGC CCGGGAAAAA CTGGAGAAGC AGCAGCAGCT TCACATCGTT 
GACCTGCTGG ACAAGGAGAT CCAGGAGCTG CAGGCTAAAC CCGAGCGCAC GGCCGAGGAC 
AACGACCGCC TGCGCAAACT CATGCTGGAG TGGCAGTTCC AGAAGAGACT GCAGGAGTCC 
AAGCAGAACG AGGAGGACGA GGAGGAGGAA GACGATGAAG ACGTGGACAC CACGCTCATC 
ATGCAGAGAC TGGAGGCCGA GAAGAGAGCA CGGCTGCAGG ACGAGGAGCG ACGACGGAAG 
CAGCAGCTGG AGGAAATCCG CAAGCGCGAA GCAGAAGAGC GAGCCAAACA GGAGGAGGAG 
AGACGCTGGA GAGAGGAGGA GAGAGCCAGG AGAGAGGCTG ACGAGAAGAG GAGACAGGAG 
GAAGAGTACT ACACCCGCCT GGAGGCAGAG CGGCGCCGGC AGCACGAGGA AGCTGAGCGC 
AAGCTGCCGA CGCCAGATGA GACGGGATTT AATTCATACA CTGGAAACAC AACAGGACTA 
GTATCAGGAG AGGTGTATAA AGACCCGCGA GACAAGTGGA CCAAAGGCCC GGATCAAGAG 
AACACAATAG GAGGAGCTCC AGAAAACCTG ACCTTCAAGG AGCGCCAGAG ACTCTTCTCG 
CAGGGGAAAG AGGTCTCCAA TAAAGTCAAA GCGTCCCGAA AGCTGATGGA GCTGGAGAAC 
GAACTCAACA TAAAACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324369.1
CDS	280..5520
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468694.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468694.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAG 
AGGAAACTGG ACTTGGATGA GAAACCACTG GTCGTGCAGC TGAACTGGAA TAAAGACGAC 
AGAGAGGGCC GCTTCGTTCT CAAGAACGAG AATGACATTA TGCCCAAGAG AACACTGACA 
AACGGTCCTG AAAAGGAGAA AGAGGGGGTG ATCCAGAACT TCAAAAGAAC ACTGTCCAAG 
AAGGAGAAGA AGAAAGAGAA GAAGAGGGAG AAGGAGGCTT TTTCCAGAAT CCCAGATGGA 
GACGATCATG TGTTGAACCG CGAGGATGGA GAGAACTCCA GATTAGCAGC TGAGGTTTAT 
AAAGACATGC CGGAGACCAG TTTCACAAGG ACCATTTCTA ACCCCGAGGT GGTGATGAAG 
AGAAGACGAC AGCAGAAGCT GGAGAAGAGG ATGCAGGAGT TTCGGAGTGA AGACGGTCGA 
CCCGACTCAG GAGGCACTCT GAGGATCTAC GCTGATAGTC TGAAGCCCAA CATTCCCTAC 
AAGACCATCC TGCTCTCAAC TACTGACATG GCTGACTTTG CTGTGGCCGA GGCTCTGGAG 
AAATATGGCC TGGAGAAGGA AAACCCTAGA GACTACTGCA TCGCCCAGGT GATTCATGCA 
AATGATAAAG CAGTGAAGGA GACCATATTA GATGACACAG ACTGTCCTCT GCAGATCCTC 
AGAGACTGGA CGAGTGATAA AGAGGCCTTA GTTTTCCAGC TGAAGAAGCG TCCTCCAGAT 
TACGCTCCAA GGAAAGGACG TAAGCCAGAC AACCGGATGG GGCCGCAGCA CGGATCCATT 
CCTCCTGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG ATGGCTCCGA CTCCAGGGAC 
AAACCCAAAC TCTACAGACT ACAGCACAGC GTTACTGAGG TCGGATCTGA CCGGAGTGAA 
GATGGAAACA TCCAGCTGTT TGGGCCTGGG ATTCTCCCTC ATCACTGTGA CCTTATGCAT 
GCGGACGGCT TGGTGACCAT AACCCCAGCC AATATGGACG CAGAGACATT TGTGGATGGA 
CAGCGGATCG CTGACACTAC AGTTCTGCGC AGTGGGGTGA CCGTCCAGTT CGGAGCAACT 
CACGTCTTCA AATTCGTAGA CCCCAGTTAT GACCAGAGCA TGTCCAAAAG AGACCCCGGG 
CCCATGATGA AAGGCAGGCA CAAATCAGGG AGTGTCCCAG AGACGACCTT TGACCTCCAC 
GGGGACATTC ACAGTGGCTC TGCTCTCCCT ACAAGCAAGA GCTCAGGGAA GCTGGAGATG 
GAGCGGTCAG GAAACAGCAG TGAAAGAGGC CTGGTGAAGC CCATGATTCG TGGAGAGCAG 
CAGGACTTTA GCAAGCATGA TGGAAGGTCT CAGGACGGCC CGGAGCTCAC TCTGCCCGCC 
AGCATCGAGT TCAGGGAAAA CTCCGAGGAT GCCTTCCTGT CGGCCATCAT CAACTACACC 
AACAGCTCGA CAGTGCACTT CAAGCTTTCT CCCACATACG TGCTGTACAT GACCTGCCGT 
TATGTCATGT CCAGCCAATA CCGGCCCGAC ATCAGTCCCT CCGAACGCAC ACACAAAGTC 
ATCGCCATTG TCAACAAGAT GGTGAGCATG ATGGAAGGAG TCATCCAGAA ACAGAAGAAC 
ATCGCTGGAG CTCTGGCGTT TTGGATGGCG AACGCTTCTG AGCTGCTGAA CTTCATCAAA 
CAGGACAAGG ATCTGAGTCG CATCACGCTG GATGCTCAGG ATGTGCTCGC TCATCTTGTT 
CAGATGGCCT TCAAGTACCT GGTGCACTGT CTGCAGTCAG ATCTCAATAA CTACATGCCA 
GCTTTCCTGG AAGATCCCGA CGAGCAAAAC CCACAGAGGC CTAAGATAGA GGATGTTCTG 
CACACGCTGA CGGGTGCCAT GTCTCTGCTG CGCCGATGCC GTGTGAACGC GGCGCTCACC 
ATCCAACTGT TTTCGCAGCT CTTCCACTTC GTCAACATGT GGCTCTTTAA CAAGCTGGTG 
ACGGAGGCCG ATTCTGGCCT GTGCTCACAC TACTGGGGCG CCATCCTTCG CCAGCAGCTC 
AGTCATATTG AGGCCTGGGC TGAGAAACAA GGCTTGGAGC TGGCTGCAGA CTGCCACCTC 
AGCCGCATAG TCCAGGCGAC GACCCTCCTT ACTATGGACA AGTACTCCAT GCAAGATGTC 
CAGAACATCA ACAACACCTG CTTCAAGCTC AACTCTCTTC AGCTCAACGC TCTGCTGAGC 
AACTACCACT GCGCTCCTGA CGAACCCTAC ATTCCTCCTG AGCTGATTGA TCACGTGGTG 
GCCGTGGCCG AAAACACTGC AGACGAACTG GCCCGAAGCG ACGGGCGGGA GGTTCAGCTG 
GAAGAGGACC CCGACCTTCA GCTGCCTTTC TTGCTGCCTG AGGACGGCTA CTCGTGCGAT 
GTGGTGCGCA ACATTCCCAA CGGATTCCAA GAGTTTCTAG AGCCACTTTG CCAGAGAGGT 
TTCTGTAGAT TGACTCCTCA TCCACGCTCG CCGGGAACTT GGACCATTTA TTTTGAAGGA 
GCCAACTGTG ACAGTCACTT CTCAGCCGAC ACCTCAGAAC TGCCAATGCG GAAGGAACCG 
GAAATTGTGA CCGTGACTTT GAAGAAACAT AATGGAATGG GCCTCAGTAT TGTGGCGGCC 
AAGGGTGCCG GGCAGGACAA ACTTGGTATT TACATCAAAT CTGTTGTCAA AGGAGGTGCA 
GCAGACATGG ATGGGCGTCT GGCTGCTGGA GACCAGTTGC TTAGCGTGGA TGGACGTAGT 
CTTGTCGGCC TCTCGCAGGA GAGAGCGGCA GAATTGATGA CACGAACTGG GTCTGTTGTA 
ACGCTTGAGG TGGCCAAACA AGGAGCCATT TATCACGGTC TGGCCACACT TCTTAACCAG 
CCATCCCCCA TGATGCCTCG CGCGTCAGAC CGTGGACGTG GCGACAAAGG AAAGCTGAGA 
CCAAAGAGTG AGGGCTTTGA GCTCTACAAC AACTCTGTAC AGAACGGCTC ACCTGAGAGC 
CCACAGGGCA CGTGGGACAC CTATCCTGAA CCAAAGAAAG AGGACAGGTT GTTGAAGAAC 
CGAGCAGACC ATCGATCCAG CCCAAATGTG GCCAATCAGG CTCAGAGTCC AGGCGGGAAG 
CCTGTTTATC CGGGAGCTCC AGGAAAGATC ACTTCGGTCT CGACGGGTAA CCTCTGCCCT 
GATGAGGAGC CCTCTCCACC CCGTCCAGAG GCCTATCCCA TCCCCACTCA GACTTACCCT 
AGGGAATACT TCACCTTCCC AGCCTCCAAA TCTCAGGACA GGATGGGCCC TGGCCAGCCC 
TGGCAAAACA ACGAACAAGA GCCTCTTCCC CCTATGGATA ACCACTCCAA CAACAGCATG 
GTTATGCAGC GGGTGGCGCG ATCTCAGGAG GAGCTGTGTG ACATGCCGGG CGGGTACCCA 
GGTGAACGTC TGCGCCAGGA AGATCTAATG CTCCTCCAGC AGCAGCAGCA GCGTGAAGCT 
GAGTACCAGC GAGGTGTTGG AGAACACTGG AACCACCAGG TGTCTTCCTC AGTGGAGTCC 
AGCACCTCCA GCCAAGAGCA CCTCAACTTC TCCTCATCCT CTGGCTCCAG GACCCAGGGC 
AACCACAAGA GTGGCCCAGG CCGCTGGAAG ACCCCACTTG CACCTCATTC CGGCCCTGTC 
TCCCAACCCT CCCGTTCAGA CCTTCCTCCA CCTCCACCTC CTCCACCAGC ACACTACGAT 
TTTGAGCCGC AAACCGACCT GCCGCTTCCA CCGCCACCTT CAGCTACACA ACAACAGCAG 
CAGGCTGCAG CCGACCGCAA GAAACGTGAA GACCAACAGC GCTGGTATGA GAAGGAAAAA 
GCTCGTCTTG AAGAGGAGCG AGAGAGGAAG AGACGAGATC AGGAAAGGAA ATTGGTGCAA 
ATCCGAAACC CTTCGGTTTC TGGTCCAGTT ATGCACAATA ACCAGCATGG CCCGGTGCCA 
CCATCTCCAC AAATGCCTCC CATGACACTT CCCCAATCAT ACCTTCCTCC AGTCCAACAA 
CCTCCGCCAC CACAACCGGC TCGACCCGAT AAACTCTCCA GTCTTCCTCG CCAGCCTCCG 
CAAGACACCG TAATCCGGGA CCTCCTGCCG CAGCAGCAGC CGCGCACCAT CGAGAGACGC 
GACCTGCAGT ACATCACCAT CAGCAAGGAG GAGTTATCGG CCAGCGACAG CCTGTCACCC 
GACCCCTGGA AGAGGGACGC CCGGGAAAAA CTGGAGAAGC AGCAGCAGCT TCACATCGTT 
GACCTGCTGG ACAAGGAGAT CCAGGAGCTG CAGGCTAAAC CCGAGCGCAC GGCCGAGGAC 
AACGACCGCC TGCGCAAACT CATGCTGGAG TGGCAGTTCC AGAAGAGACT GCAGGAGTCC 
AAGCAGAACG AGGAGGACGA GGAGGAGGAA GACGATGAAG ACGTGGACAC CACGCTCATC 
ATGCAGAGAC TGGAGGCCGA GAAGAGAGCA CGGCTGCAGG ACGAGGAGCG ACGACGGAAG 
CAGCAGCTGG AGGAAATCCG CAAGCGCGAA GCAGAAGAGC GAGCCAAACA GGAGGAGGAG 
AGACGCTGGA GAGAGGAGGA GAGAGCCAGG AGAGAGGCTG ACGAGAAGAG GAGACAGGAG 
GAAGAGTACT ACACCCGCCT GGAGGCAGAG CGGCGCCGGC AGCACGAGGA AGCTGAGCGC 
AAGCTGCCGA CGCCAGATGA GACGGGATTT AATTCATACA CTGGAAACAC AACAGGACTA 
GTATCAGGAG AGGTGTATAA AGACCCGCGA GACAAGTGGA CCAAAGGCCC GGATCAAGAG 
AACACAATAG GAGGAGCTCC AGAAAACCTG ACCTTCAAGG AGCGCCAGAG ACTCTTCTCG 
CAGGGGAAAG AGGTCTCCAA TAAAGTCAAA GCGTCCCGAA AGCTGATGGA GCTGGAGAAC 
GAACTCAACA TAAAACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468695.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324370.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X20, mRNA.

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CloneID	ODa150791
Clone ID Related Accession (Same CDS sequence)	XM_021468695.1
Accession Version	XM_021468695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAG 
AGGAAACTGG ACTTGGATGA GAAACCACTG GTCGTGCAGC TGAACTGGAA TAAAGACGAC 
AGAGAGGGCC GCTTCGTTCT CAAGAACGAG AATGACATTA TGCCCAAGAG AACACTGACA 
AACGGTCCTG AAAAGGAGAA AGAGGGGGTG ATCCAGAACT TCAAAAGAAC ACTGTCCAAG 
AAGGAGAAGA AGAAAGAGAA GAAGAGGGAG AAGGAGGCTT TTTCCAGAAT CCCAGATGGA 
GACGATCATG TGTTGAACCG CGAGGATGGA GAGAACTCCA GATTAGCAGC TGAGGTTTAT 
AAAGACATGC CGGAGACCAG TTTCACAAGG ACCATTTCTA ACCCCGAGGT GGTGATGAAG 
AGAAGACGAC AGCAGAAGCT GGAGAAGAGG ATGCAGGAGT TTCGGAGTGA AGACGGTCGA 
CCCGACTCAG GAGGCACTCT GAGGATCTAC GCTGATAGTC TGAAGCCCAA CATTCCCTAC 
AAGACCATCC TGCTCTCAAC TACTGACATG GCTGACTTTG CTGTGGCCGA GGCTCTGGAG 
AAATATGGCC TGGAGAAGGA AAACCCTAGA GACTACTGCA TCGCCCAGGT GATTCATGCA 
AATGATAAAG CAGTGAAGGA GACCATATTA GATGACACAG ACTGTCCTCT GCAGATCCTC 
AGAGACTGGA CGAGTGATAA AGAGGCCTTA GTTTTCCAGC TGAAGAAGCG TCCTCCAGAT 
TACGCTCCAA GGAAAGGACG TAAGCCAGAC AACCGGATGG GGCCGCAGCA CGGATCCATT 
CCTCCTGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAG TCACTATGCC 
TACTACTACC GCCATCATGA AGATGGCTCC GACTCCAGGG ACAAACCCAA ACTCTACAGA 
CTACAGCACA GCGTTACTGA GGTCGGATCT GACCGGAGTG AAGATGGAAA CATCCAGCTG 
TTTGGGCCTG GGATTCTCCC TCATCACTGT GACCTTATGC ATGCGGACGG CTTGGTGACC 
ATAACCCCAG CCAATATGGA CGCAGAGACA TTTGTGGATG GACAGCGGAT CGCTGACACT 
ACAGTTCTGC GCAGTGGGGT GACCGTCCAG TTCGGAGCAA CTCACGTCTT CAAATTCGTA 
GACCCCAGTT ATGACCAGAG CATGTCCAAA AGAGACCCCG GGCCCATGAT GAAAGGCAGG 
CACAAATCAG GGAGTGTCCC AGAGACGACC TTTGACCTCC ACGGGGACAT TCACAGTGGC 
TCTGCTCTCC CTACAAGCAA GAGCTCAGGG AAGCTGGAGA TGGAGCGGTC AGGAAACAGC 
AGTGAAAGAG GCCTGGTGAA GCCCATGATT CGTGGAGAGC AGCAGGACTT TAGCAAGCAT 
GATGGAAGGT CTCAGGACGG CCCGGAGCTC ACTCTGCCCG CCAGCATCGA GTTCAGGGAA 
AACTCCGAGG ATGCCTTCCT GTCGGCCATC ATCAACTACA CCAACAGCTC GACAGTGCAC 
TTCAAGCTTT CTCCCACATA CGTGCTGTAC ATGACCTGCC GTTATGTCAT GTCCAGCCAA 
TACCGGCCCG ACATCAGTCC CTCCGAACGC ACACACAAAG TCATCGCCAT TGTCAACAAG 
ATGGTGAGCA TGATGGAAGG AGTCATCCAG AAACAGAAGA ACATCGCTGG AGCTCTGGCG 
TTTTGGATGG CGAACGCTTC TGAGCTGCTG AACTTCATCA AACAGGACAA GGATCTGAGT 
CGCATCACGC TGGATGCTCA GGATGTGCTC GCTCATCTTG TTCAGATGGC CTTCAAGTAC 
CTGGTGCACT GTCTGCAGTC AGATCTCAAT AACTACATGC CAGCTTTCCT GGAAGATCCC 
GACGAGCAAA ACCCACAGAG GCCTAAGATA GAGGATGTTC TGCACACGCT GACGGGTGCC 
ATGTCTCTGC TGCGCCGATG CCGTGTGAAC GCGGCGCTCA CCATCCAACT GTTTTCGCAG 
CTCTTCCACT TCGTCAACAT GTGGCTCTTT AACAAGCTGG TGACGGAGGC CGATTCTGGC 
CTGTGCTCAC ACTACTGGGG CGCCATCCTT CGCCAGCAGC TCAGTCATAT TGAGGCCTGG 
GCTGAGAAAC AAGGCTTGGA GCTGGCTGCA GACTGCCACC TCAGCCGCAT AGTCCAGGCG 
ACGACCCTCC TTACTATGGA CAAGTACTCC ATGCAAGATG TCCAGAACAT CAACAACACC 
TGCTTCAAGC TCAACTCTCT TCAGCTCAAC GCTCTGCTGA GCAACTACCA CTGCGCTCCT 
GACGAACCCT ACATTCCTCC TGAGCTGATT GATCACGTGG TGGCCGTGGC CGAAAACACT 
GCAGACGAAC TGGCCCGAAG CGACGGGCGG GAGGTTCAGC TGGAAGAGGA CCCCGACCTT 
CAGCTGCCTT TCTTGCTGCC TGAGGACGGC TACTCGTGCG ATGTGGTGCG CAACATTCCC 
AACGGATTCC AAGAGTTTCT AGAGCCACTT TGCCAGAGAG GTTTCTGTAG ATTGACTCCT 
CATCCACGCT CGCCGGGAAC TTGGACCATT TATTTTGAAG GAGCCAACTG TGACAGTCAC 
TTCTCAGCCG ACACCTCAGA ACTGCCAATG CGGAAGGAAC CGGAAATTGT GACCGTGACT 
TTGAAGAAAC ATAATGGAAT GGGCCTCAGT ATTGTGGCGG CCAAGGGTGC CGGGCAGGAC 
AAACTTGGTA TTTACATCAA ATCTGTTGTC AAAGGAGGTG CAGCAGACAT GGATGGGCGT 
CTGGCTGCTG GAGACCAGTT GCTTAGCGTG GATGGACGTA GTCTTGTCGG CCTCTCGCAG 
GAGAGAGCGG CAGAATTGAT GACACGAACT GGGTCTGTTG TAACGCTTGA GGTGGCCAAA 
CAAGGAGCCA TTTATCACGG TCTGGCCACA CTTCTTAACC AGCCATCCCC CATGATGCCT 
CGCGCGTCAG ACCGTGGACG TGGCGACAAA GGAAAGCTGA GACCAAAGAG TGAGGGCTTT 
GAGCTCTACA ACAACTCTGT ACAGAACGGC TCACCTGAGA GCCCACAGGG CACGTGGGAC 
ACCTATCCTG AACCAAAGAA AGAGGACAGG TTGTTGAAGA ACCGAGCAGA CCATCGATCC 
AGCCCAAATG TGGCCAATCA GGCTCAGAGT CCAGGCGGGA AGCCTGTTTA TCCGGGAGCT 
CCAGGAAAGA TCACTTCGGT CTCGACGGGT AACCTCTGCC CTGATGAGGA GCCCTCTCCA 
CCCCGTCCAG AGGCCTATCC CATCCCCACT CAGACTTACC CTAGGGAATA CTTCACCTTC 
CCAGCCTCCA AATCTCAGGA CAGGATGGGC CCTGGCCAGC CCTGGCAAAA CAACGAACAA 
GAGCCTCTTC CCCCTATGGA TAACCACTCC AACAACAGCA TGGTTATGCA GCGGGTGGCG 
CGATCTCAGG AGGAGCTGTG TGACATGCCG GGCGGGTACC CAGGTGAACG TCTGCGCCAG 
GAAGATCTAA TGCTCCTCCA GCAGCAGCAG CAGCGTGAAG CTGAGTACCA GCGAGGTGTT 
GGAGAACACT GGAACCACCA GGTGTCTTCC TCAGTGGAGT CCAGCACCTC CAGCCAAGAG 
CACCTCAACT TCTCCTCATC CTCTGGCTCC AGGACCCAGG GCAACCACAA GAGTGGCCCA 
GGCCGCTGGA AGACCCCACT TGCACCTCAT TCCGGCCCTG TCTCCCAACC CTCCCGTTCA 
GACCTTCCTC CACCTCCACC TCCTCCACCA GCACACTACG ATTTTGAGCC GCAAACCGAC 
CTGCCGCTTC CACCGCCACC TTCAGCTACA CAACAACAGC AGCAGGCTGC AGCCGACCGC 
AAGAAACGTG AAGACCAACA GCGCTGGTAT GAGAAGGAAA AAGCTCGTCT TGAAGAGGAG 
CGAGAGAGGA AGAGACGAGA TCAGGAAAGG AAATTGGTGC AAATCCGAAA CCCTTCGGTT 
TCTGGTCCAG TTATGCACAA TAACCAGCAT GGCCCGGTGC CACCATCTCC ACAAATGCCT 
CCCATGACAC TTCCCCAATC ATACCTTCCT CCAGTCCAAC AACCTCCGCC ACCACAACCG 
GCTCGACCCG ATAAACTCTC CAGTCTTCCT CGCCAGCCTC CGCAAGACAC CGTAATCCGG 
GACCTCCTGC CGCAGCAGCA GCCGCGCACC ATCGAGAGAC GCGACCTGCA GTACATCACC 
ATCAGCAAGG AGGAGTTATC GGCCAGCGAC AGCCTGTCAC CCGACCCCTG GAAGAGGGAC 
GCCCGGGAAA AACTGGAGAA GCAGCAGCAG CTTCACATCG TTGACCTGCT GGACAAGGAG 
ATCCAGGAGC TGCAGGCTAA ACCCGAGCGC ACGGCCGAGG ACAACGACCG CCTGCGCAAA 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTGCAGGAGT CCAAGCAGAA CGAGGAGGAC 
GAGGAGGAGG AAGACGATGA AGACGTGGAC ACCACGCTCA TCATGCAGAG ACTGGAGGCC 
GAGAAGAGAG CACGGCAGAC TGCTGTTCCA GCAATCTCTG TACTAGACTT GCTGCAGGAC 
GAGGAGCGAC GACGGAAGCA GCAGCTGGAG GAAATCCGCA AGCGCGAAGC AGAAGAGCGA 
GCCAAACAGG AGGAGGAGAG ACGCTGGAGA GAGGAGGAGA GAGCCAGGAG AGAGGCTGAC 
GAGAAGAGGA GACAGGAGGA AGAGTACTAC ACCCGCCTGG AGGCAGAGCG GCGCCGGCAG 
CACGAGGAAG CTGAGCGCAA GCTGCCGACG CCAGATGAGA CGGGTCTGTA CAGACCCCCT 
TTACCCCGGG ACTACCAGCC CCCTTCTCCC TCAAGCGCAC CCGCCACTAA CCACACCACC 
AGCGCCCCCC CGCCTCCGCC TCAGAGAAAC ACCTCCTACC TCAAAACCCA GGTCGCCTCC 
CCAGACACCA TTTACACTGC CAAGTTTGTC TCGTACAATG ACGAGGAGGA GGAGGAGGAT 
GAGGAAGATG CGGGCCTAAC AGGTCAGGTT AAGCTCTCGG CCACCCGCAA GTCCTACGGC 
GACCTTCCGC CTGCCCCAAA GCCCCAGCCG CCGCCAACCG CGCGCAAGCC CCGTCCCGTC 
TCAGATGGCA TCTTTCTGTC CAACTCATTC CAGCTGCCCG CTGGGCTCAA TGCCAACAGT 
ACTGCTCTCA AGACAGGGCA GCCCCCTCTC CCCTTCACCA AACCCAGCTT CATCCCATCA 
GCCAACTCCA AAGGATTTAA TTCATACACT GGAAACACAA CAGGACTAGT ATCAGGAGAG 
GTGTATAAAG ACCCGCGAGA CAAGTGGACC AAAGGCAGCC CGGATCAAGA GAACACAATA 
GGAGGAGCTC CAGAAAACCT GACCTTCAAG GAGCGCCAGA GACTCTTCTC GCAGGGGAAA 
GAGGTCTCCA ATAAAGTCAA AGCGTCCCGA AAGCTGATGG AGCTGGAGAA CGAACTCAAC 
ATAAAACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324370.1
CDS	280..6051
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468695.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468695.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAG 
AGGAAACTGG ACTTGGATGA GAAACCACTG GTCGTGCAGC TGAACTGGAA TAAAGACGAC 
AGAGAGGGCC GCTTCGTTCT CAAGAACGAG AATGACATTA TGCCCAAGAG AACACTGACA 
AACGGTCCTG AAAAGGAGAA AGAGGGGGTG ATCCAGAACT TCAAAAGAAC ACTGTCCAAG 
AAGGAGAAGA AGAAAGAGAA GAAGAGGGAG AAGGAGGCTT TTTCCAGAAT CCCAGATGGA 
GACGATCATG TGTTGAACCG CGAGGATGGA GAGAACTCCA GATTAGCAGC TGAGGTTTAT 
AAAGACATGC CGGAGACCAG TTTCACAAGG ACCATTTCTA ACCCCGAGGT GGTGATGAAG 
AGAAGACGAC AGCAGAAGCT GGAGAAGAGG ATGCAGGAGT TTCGGAGTGA AGACGGTCGA 
CCCGACTCAG GAGGCACTCT GAGGATCTAC GCTGATAGTC TGAAGCCCAA CATTCCCTAC 
AAGACCATCC TGCTCTCAAC TACTGACATG GCTGACTTTG CTGTGGCCGA GGCTCTGGAG 
AAATATGGCC TGGAGAAGGA AAACCCTAGA GACTACTGCA TCGCCCAGGT GATTCATGCA 
AATGATAAAG CAGTGAAGGA GACCATATTA GATGACACAG ACTGTCCTCT GCAGATCCTC 
AGAGACTGGA CGAGTGATAA AGAGGCCTTA GTTTTCCAGC TGAAGAAGCG TCCTCCAGAT 
TACGCTCCAA GGAAAGGACG TAAGCCAGAC AACCGGATGG GGCCGCAGCA CGGATCCATT 
CCTCCTGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAG TCACTATGCC 
TACTACTACC GCCATCATGA AGATGGCTCC GACTCCAGGG ACAAACCCAA ACTCTACAGA 
CTACAGCACA GCGTTACTGA GGTCGGATCT GACCGGAGTG AAGATGGAAA CATCCAGCTG 
TTTGGGCCTG GGATTCTCCC TCATCACTGT GACCTTATGC ATGCGGACGG CTTGGTGACC 
ATAACCCCAG CCAATATGGA CGCAGAGACA TTTGTGGATG GACAGCGGAT CGCTGACACT 
ACAGTTCTGC GCAGTGGGGT GACCGTCCAG TTCGGAGCAA CTCACGTCTT CAAATTCGTA 
GACCCCAGTT ATGACCAGAG CATGTCCAAA AGAGACCCCG GGCCCATGAT GAAAGGCAGG 
CACAAATCAG GGAGTGTCCC AGAGACGACC TTTGACCTCC ACGGGGACAT TCACAGTGGC 
TCTGCTCTCC CTACAAGCAA GAGCTCAGGG AAGCTGGAGA TGGAGCGGTC AGGAAACAGC 
AGTGAAAGAG GCCTGGTGAA GCCCATGATT CGTGGAGAGC AGCAGGACTT TAGCAAGCAT 
GATGGAAGGT CTCAGGACGG CCCGGAGCTC ACTCTGCCCG CCAGCATCGA GTTCAGGGAA 
AACTCCGAGG ATGCCTTCCT GTCGGCCATC ATCAACTACA CCAACAGCTC GACAGTGCAC 
TTCAAGCTTT CTCCCACATA CGTGCTGTAC ATGACCTGCC GTTATGTCAT GTCCAGCCAA 
TACCGGCCCG ACATCAGTCC CTCCGAACGC ACACACAAAG TCATCGCCAT TGTCAACAAG 
ATGGTGAGCA TGATGGAAGG AGTCATCCAG AAACAGAAGA ACATCGCTGG AGCTCTGGCG 
TTTTGGATGG CGAACGCTTC TGAGCTGCTG AACTTCATCA AACAGGACAA GGATCTGAGT 
CGCATCACGC TGGATGCTCA GGATGTGCTC GCTCATCTTG TTCAGATGGC CTTCAAGTAC 
CTGGTGCACT GTCTGCAGTC AGATCTCAAT AACTACATGC CAGCTTTCCT GGAAGATCCC 
GACGAGCAAA ACCCACAGAG GCCTAAGATA GAGGATGTTC TGCACACGCT GACGGGTGCC 
ATGTCTCTGC TGCGCCGATG CCGTGTGAAC GCGGCGCTCA CCATCCAACT GTTTTCGCAG 
CTCTTCCACT TCGTCAACAT GTGGCTCTTT AACAAGCTGG TGACGGAGGC CGATTCTGGC 
CTGTGCTCAC ACTACTGGGG CGCCATCCTT CGCCAGCAGC TCAGTCATAT TGAGGCCTGG 
GCTGAGAAAC AAGGCTTGGA GCTGGCTGCA GACTGCCACC TCAGCCGCAT AGTCCAGGCG 
ACGACCCTCC TTACTATGGA CAAGTACTCC ATGCAAGATG TCCAGAACAT CAACAACACC 
TGCTTCAAGC TCAACTCTCT TCAGCTCAAC GCTCTGCTGA GCAACTACCA CTGCGCTCCT 
GACGAACCCT ACATTCCTCC TGAGCTGATT GATCACGTGG TGGCCGTGGC CGAAAACACT 
GCAGACGAAC TGGCCCGAAG CGACGGGCGG GAGGTTCAGC TGGAAGAGGA CCCCGACCTT 
CAGCTGCCTT TCTTGCTGCC TGAGGACGGC TACTCGTGCG ATGTGGTGCG CAACATTCCC 
AACGGATTCC AAGAGTTTCT AGAGCCACTT TGCCAGAGAG GTTTCTGTAG ATTGACTCCT 
CATCCACGCT CGCCGGGAAC TTGGACCATT TATTTTGAAG GAGCCAACTG TGACAGTCAC 
TTCTCAGCCG ACACCTCAGA ACTGCCAATG CGGAAGGAAC CGGAAATTGT GACCGTGACT 
TTGAAGAAAC ATAATGGAAT GGGCCTCAGT ATTGTGGCGG CCAAGGGTGC CGGGCAGGAC 
AAACTTGGTA TTTACATCAA ATCTGTTGTC AAAGGAGGTG CAGCAGACAT GGATGGGCGT 
CTGGCTGCTG GAGACCAGTT GCTTAGCGTG GATGGACGTA GTCTTGTCGG CCTCTCGCAG 
GAGAGAGCGG CAGAATTGAT GACACGAACT GGGTCTGTTG TAACGCTTGA GGTGGCCAAA 
CAAGGAGCCA TTTATCACGG TCTGGCCACA CTTCTTAACC AGCCATCCCC CATGATGCCT 
CGCGCGTCAG ACCGTGGACG TGGCGACAAA GGAAAGCTGA GACCAAAGAG TGAGGGCTTT 
GAGCTCTACA ACAACTCTGT ACAGAACGGC TCACCTGAGA GCCCACAGGG CACGTGGGAC 
ACCTATCCTG AACCAAAGAA AGAGGACAGG TTGTTGAAGA ACCGAGCAGA CCATCGATCC 
AGCCCAAATG TGGCCAATCA GGCTCAGAGT CCAGGCGGGA AGCCTGTTTA TCCGGGAGCT 
CCAGGAAAGA TCACTTCGGT CTCGACGGGT AACCTCTGCC CTGATGAGGA GCCCTCTCCA 
CCCCGTCCAG AGGCCTATCC CATCCCCACT CAGACTTACC CTAGGGAATA CTTCACCTTC 
CCAGCCTCCA AATCTCAGGA CAGGATGGGC CCTGGCCAGC CCTGGCAAAA CAACGAACAA 
GAGCCTCTTC CCCCTATGGA TAACCACTCC AACAACAGCA TGGTTATGCA GCGGGTGGCG 
CGATCTCAGG AGGAGCTGTG TGACATGCCG GGCGGGTACC CAGGTGAACG TCTGCGCCAG 
GAAGATCTAA TGCTCCTCCA GCAGCAGCAG CAGCGTGAAG CTGAGTACCA GCGAGGTGTT 
GGAGAACACT GGAACCACCA GGTGTCTTCC TCAGTGGAGT CCAGCACCTC CAGCCAAGAG 
CACCTCAACT TCTCCTCATC CTCTGGCTCC AGGACCCAGG GCAACCACAA GAGTGGCCCA 
GGCCGCTGGA AGACCCCACT TGCACCTCAT TCCGGCCCTG TCTCCCAACC CTCCCGTTCA 
GACCTTCCTC CACCTCCACC TCCTCCACCA GCACACTACG ATTTTGAGCC GCAAACCGAC 
CTGCCGCTTC CACCGCCACC TTCAGCTACA CAACAACAGC AGCAGGCTGC AGCCGACCGC 
AAGAAACGTG AAGACCAACA GCGCTGGTAT GAGAAGGAAA AAGCTCGTCT TGAAGAGGAG 
CGAGAGAGGA AGAGACGAGA TCAGGAAAGG AAATTGGTGC AAATCCGAAA CCCTTCGGTT 
TCTGGTCCAG TTATGCACAA TAACCAGCAT GGCCCGGTGC CACCATCTCC ACAAATGCCT 
CCCATGACAC TTCCCCAATC ATACCTTCCT CCAGTCCAAC AACCTCCGCC ACCACAACCG 
GCTCGACCCG ATAAACTCTC CAGTCTTCCT CGCCAGCCTC CGCAAGACAC CGTAATCCGG 
GACCTCCTGC CGCAGCAGCA GCCGCGCACC ATCGAGAGAC GCGACCTGCA GTACATCACC 
ATCAGCAAGG AGGAGTTATC GGCCAGCGAC AGCCTGTCAC CCGACCCCTG GAAGAGGGAC 
GCCCGGGAAA AACTGGAGAA GCAGCAGCAG CTTCACATCG TTGACCTGCT GGACAAGGAG 
ATCCAGGAGC TGCAGGCTAA ACCCGAGCGC ACGGCCGAGG ACAACGACCG CCTGCGCAAA 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTGCAGGAGT CCAAGCAGAA CGAGGAGGAC 
GAGGAGGAGG AAGACGATGA AGACGTGGAC ACCACGCTCA TCATGCAGAG ACTGGAGGCC 
GAGAAGAGAG CACGGCAGAC TGCTGTTCCA GCAATCTCTG TACTAGACTT GCTGCAGGAC 
GAGGAGCGAC GACGGAAGCA GCAGCTGGAG GAAATCCGCA AGCGCGAAGC AGAAGAGCGA 
GCCAAACAGG AGGAGGAGAG ACGCTGGAGA GAGGAGGAGA GAGCCAGGAG AGAGGCTGAC 
GAGAAGAGGA GACAGGAGGA AGAGTACTAC ACCCGCCTGG AGGCAGAGCG GCGCCGGCAG 
CACGAGGAAG CTGAGCGCAA GCTGCCGACG CCAGATGAGA CGGGTCTGTA CAGACCCCCT 
TTACCCCGGG ACTACCAGCC CCCTTCTCCC TCAAGCGCAC CCGCCACTAA CCACACCACC 
AGCGCCCCCC CGCCTCCGCC TCAGAGAAAC ACCTCCTACC TCAAAACCCA GGTCGCCTCC 
CCAGACACCA TTTACACTGC CAAGTTTGTC TCGTACAATG ACGAGGAGGA GGAGGAGGAT 
GAGGAAGATG CGGGCCTAAC AGGTCAGGTT AAGCTCTCGG CCACCCGCAA GTCCTACGGC 
GACCTTCCGC CTGCCCCAAA GCCCCAGCCG CCGCCAACCG CGCGCAAGCC CCGTCCCGTC 
TCAGATGGCA TCTTTCTGTC CAACTCATTC CAGCTGCCCG CTGGGCTCAA TGCCAACAGT 
ACTGCTCTCA AGACAGGGCA GCCCCCTCTC CCCTTCACCA AACCCAGCTT CATCCCATCA 
GCCAACTCCA AAGGATTTAA TTCATACACT GGAAACACAA CAGGACTAGT ATCAGGAGAG 
GTGTATAAAG ACCCGCGAGA CAAGTGGACC AAAGGCAGCC CGGATCAAGA GAACACAATA 
GGAGGAGCTC CAGAAAACCT GACCTTCAAG GAGCGCCAGA GACTCTTCTC GCAGGGGAAA 
GAGGTCTCCA ATAAAGTCAA AGCGTCCCGA AAGCTGATGG AGCTGGAGAA CGAACTCAAC 
ATAAAACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_021468676.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324351.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X1, mRNA.

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CloneID	ODa42826
Clone ID Related Accession (Same CDS sequence)	XM_021468676.1
Accession Version	XM_021468676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGTC TGTACAGACC CCCTTTACCC CGGGACTACC AGCCCCCTTC TCCCTCAAGC 
GCACCCGCCA CTAACCACAC CACCAGCGCC CCCCCGCCTC CGCCTCAGAG AAACACCTCC 
TACCTCAAAA CCCAGGTCGC CTCCCCAGAC ACCATTTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGAGGAGGA GGATGAGGAA GATGCGGGCC TAACAGGTCA GGTTAAGCTC 
TCGGCCACCC GCAAGTCCTA CGGCGACCTT CCGCCTGCCC CAAAGCCCCA GCCGCCGCCA 
ACCGCGCGCA AGCCCCGTCC CGTCTCAGAT GGCATCTTTC TGTCCAACTC ATTCCAGCTG 
CCCGCTGGGC TCAATGCCAA CAGTACTGCT CTCAAGACAG GGCAGCCCCC TCTCCCCTTC 
ACCAAACCCA GCTTCATCCC ATCAGCCAAC TCCAAAGGAT TTAATTCATA CACTGGAAAC 
ACAACAGGAC TAGTATCAGG AGAGGTGTAT AAAGACCCGC GAGACAAGTG GACCAAAGGC 
AGCCCGGATC AAGAGAACAC AATAGGAGGA GCTCCAGAAA ACCTGACCTT CAAGGAGCGC 
CAGAGACTCT TCTCGCAGGG GAAAGAGGTC TCCAATAAAG TCAAAGCGTC CCGAAAGCTG 
ATGGAGCTGG AGAACGAACT CAACATAAAA CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324351.1
CDS	281..6076
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_021468676.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468676.1

ORF Insert Sequence:

ATGTCGGGGA GCCGAGAGGA GGAGCGGCGG AAACTCGCCG ACATCATCAA CCACTGGAAC 
GCAAACCGGC TCGACCTGTT CGAGATCAGC CAACCCACAG AGGATTTGGA GTTCCATGGG 
GTGATGCGGT TTTACTTCCA GGACCGTGTG GCAGGGAATT TTGCCACAAA GTGCATCAGA 
GTGTCCAGTA CAGCCACCAC ACAAGATGTG ATCGAGACGC TGGCGGAGAA GTTCAGACCC 
GACATGAGGA TGCTGTCTTC TCCAAAATAC TCCCTCTATG AGGTCCACGT CAGTGGCGAA 
GAGAGGAAAC TGGACTTGGA TGAGAAACCA CTGGTCGTGC AGCTGAACTG GAATAAAGAC 
GACAGAGAGG GCCGCTTCGT TCTCAAGAAC GAGAATGACA TTATGCCCAA GAGAACACTG 
ACAAACGGTC CTGAAAAGGA GAAAGAGGGG GTGATCCAGA ACTTCAAAAG AACACTGTCC 
AAGAAGGAGA AGAAGAAAGA GAAGAAGAGG GAGAAGGAGG CTTTTTCCAG AATCCCAGAT 
GGAGACGATC ATGTGTTGAA CCGCGAGGAT GGAGAGAACT CCAGATTAGC AGCTGAGGTT 
TATAAAGACA TGCCGGAGAC CAGTTTCACA AGGACCATTT CTAACCCCGA GGTGGTGATG 
AAGAGAAGAC GACAGCAGAA GCTGGAGAAG AGGATGCAGG AGTTTCGGAG TGAAGACGGT 
CGACCCGACT CAGGAGGCAC TCTGAGGATC TACGCTGATA GTCTGAAGCC CAACATTCCC 
TACAAGACCA TCCTGCTCTC AACTACTGAC ATGGCTGACT TTGCTGTGGC CGAGGCTCTG 
GAGAAATATG GCCTGGAGAA GGAAAACCCT AGAGACTACT GCATCGCCCA GGTGATTCAT 
GCAAATGATA AAGCAGTGAA GGAGACCATA TTAGATGACA CAGACTGTCC TCTGCAGATC 
CTCAGAGACT GGACGAGTGA TAAAGAGGCC TTAGTTTTCC AGCTGAAGAA GCGTCCTCCA 
GATTACGCTC CAAGGAAAGG ACGTAAGCCA GACAACCGGA TGGGGCCGCA GCACGGATCC 
ATTCCTCCTG AGAAACTGCC TTACCTGGTG GAGCTGAGCC CAGGGCGAGG GAGTCACTAT 
GCCTACTACT ACCGCCATCA TGAAGATGGC TCCGACTCCA GGGACAAACC CAAACTCTAC 
AGACTACAGC ACAGCGTTAC TGAGGTCGGA TCTGACCGGA GTGAAGATGG AAACATCCAG 
CTGTTTGGGC CTGGGATTCT CCCTCATCAC TGTGACCTTA TGCATGCGGA CGGCTTGGTG 
ACCATAACCC CAGCCAATAT GGACGCAGAG ACATTTGTGG ATGGACAGCG GATCGCTGAC 
ACTACAGTTC TGCGCAGTGG GGTGACCGTC CAGTTCGGAG CAACTCACGT CTTCAAATTC 
GTAGACCCCA GTTATGACCA GAGCATGTCC AAAAGAGACC CCGGGCCCAT GATGAAAGGC 
AGGCACAAAT CAGGGAGTGT CCCAGAGACG ACCTTTGACC TCCACGGGGA CATTCACAGT 
GGCTCTGCTC TCCCTACAAG CAAGAGCTCA GGGAAGCTGG AGATGGAGCG GTCAGGAAAC 
AGCAGTGAAA GAGGCCTGGT GAAGCCCATG ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG 
CATGATGGAA GGTCTCAGGA CGGCCCGGAG CTCACTCTGC CCGCCAGCAT CGAGTTCAGG 
GAAAACTCCG AGGATGCCTT CCTGTCGGCC ATCATCAACT ACACCAACAG CTCGACAGTG 
CACTTCAAGC TTTCTCCCAC ATACGTGCTG TACATGACCT GCCGTTATGT CATGTCCAGC 
CAATACCGGC CCGACATCAG TCCCTCCGAA CGCACACACA AAGTCATCGC CATTGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTG CTGAAGGTGA TCAGAAACAG 
AAGAACATCG CTGGAGCTCT GGCGTTTTGG ATGGCGAACG CTTCTGAGCT GCTGAACTTC 
ATCAAACAGG ACAAGGATCT GAGTCGCATC ACGCTGGATG CTCAGGATGT GCTCGCTCAT 
CTTGTTCAGA TGGCCTTCAA GTACCTGGTG CACTGTCTGC AGTCAGATCT CAATAACTAC 
ATGCCAGCTT TCCTGGAAGA TCCCGACGAG CAAAACCCAC AGAGGCCTAA GATAGAGGAT 
GTTCTGCACA CGCTGACGGG TGCCATGTCT CTGCTGCGCC GATGCCGTGT GAACGCGGCG 
CTCACCATCC AACTGTTTTC GCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTTAACAAG 
CTGGTGACGG AGGCCGATTC TGGCCTGTGC TCACACTACT GGGGCGCCAT CCTTCGCCAG 
CAGCTCAGTC ATATTGAGGC CTGGGCTGAG AAACAAGGCT TGGAGCTGGC TGCAGACTGC 
CACCTCAGCC GCATAGTCCA GGCGACGACC CTCCTTACTA TGGACAAGTA CTCCATGCAA 
GATGTCCAGA ACATCAACAA CACCTGCTTC AAGCTCAACT CTCTTCAGCT CAACGCTCTG 
CTGAGCAACT ACCACTGCGC TCCTGACGAA CCCTACATTC CTCCTGAGCT GATTGATCAC 
GTGGTGGCCG TGGCCGAAAA CACTGCAGAC GAACTGGCCC GAAGCGACGG GCGGGAGGTT 
CAGCTGGAAG AGGACCCCGA CCTTCAGCTG CCTTTCTTGC TGCCTGAGGA CGGCTACTCG 
TGCGATGTGG TGCGCAACAT TCCCAACGGA TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG 
AGAGGTTTCT GTAGATTGAC TCCTCATCCA CGCTCGCCGG GAACTTGGAC CATTTATTTT 
GAAGGAGCCA ACTGTGACAG TCACTTCTCA GCCGACACCT CAGAACTGCC AATGCGGAAG 
GAACCGGAAA TTGTGACCGT GACTTTGAAG AAACATAATG GAATGGGCCT CAGTATTGTG 
GCGGCCAAGG GTGCCGGGCA GGACAAACTT GGTATTTACA TCAAATCTGT TGTCAAAGGA 
GGTGCAGCAG ACATGGATGG GCGTCTGGCT GCTGGAGACC AGTTGCTTAG CGTGGATGGA 
CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA GCGGCAGAAT TGATGACACG AACTGGGTCT 
GTTGTAACGC TTGAGGTGGC CAAACAAGGA GCCATTTATC ACGGTCTGGC CACACTTCTT 
AACCAGCCAT CCCCCATGAT GCCTCGCGCG TCAGACCGTG GACGTGGCGA CAAAGGAAAG 
CTGAGACCAA AGAGTGAGGG CTTTGAGCTC TACAACAACT CTGTACAGAA CGGCTCACCT 
GAGAGCCCAC AGGGCACGTG GGACACCTAT CCTGAACCAA AGAAAGAGGA CAGGTTGTTG 
AAGAACCGAG CAGACCATCG ATCCAGCCCA AATGTGGCCA ATCAGGCTCA GAGTCCAGGC 
GGGAAGCCTG TTTATCCGGG AGCTCCAGGA AAGATCACTT CGGTCTCGAC GGGTAACCTC 
TGCCCTGATG AGGAGCCCTC TCCACCCCGT CCAGAGGCCT ATCCCATCCC CACTCAGACT 
TACCCTAGGG AATACTTCAC CTTCCCAGCC TCCAAATCTC AGGACAGGAT GGGCCCTGGC 
CAGCCCTGGC AAAACAACGA ACAAGAGCCT CTTCCCCCTA TGGATAACCA CTCCAACAAC 
AGCATGGTTA TGCAGCGGGT GGCGCGATCT CAGGAGGAGC TGTGTGACAT GCCGGGCGGG 
TACCCAGGTG AACGTCTGCG CCAGGAAGAT CTAATGCTCC TCCAGCAGCA GCAGCAGCGT 
GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA CACTGGAACC ACCAGGTGTC TTCCTCAGTG 
GAGTCCAGCA CCTCCAGCCA AGAGCACCTC AACTTCTCCT CATCCTCTGG CTCCAGGACC 
CAGGGCAACC ACAAGAGTGG CCCAGGCCGC TGGAAGACCC CACTTGCACC TCATTCCGGC 
CCTGTCTCCC AACCCTCCCG TTCAGACCTT CCTCCACCTC CACCTCCTCC ACCAGCACAC 
TACGATTTTG AGCCGCAAAC CGACCTGCCG CTTCCACCGC CACCTTCAGC TACACAACAA 
CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA CGTGAAGACC AACAGCGCTG GTATGAGAAG 
GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG AGGAAGAGAC GAGATCAGGA AAGGAAATTG 
GTGCAAATCC GAAACCCTTC GGTTTCTGGT CCAGTTATGC ACAATAACCA GCATGGCCCG 
GTGCCACCAT CTCCACAAAT GCCTCCCATG ACACTTCCCC AATCATACCT TCCTCCAGTC 
CAACAACCTC CGCCACCACA ACCGGCTCGA CCCGATAAAC TCTCCAGTCT TCCTCGCCAG 
CCTCCGCAAG ACACCGTAAT CCGGGACCTC CTGCCGCAGC AGCAGCCGCG CACCATCGAG 
AGACGCGACC TGCAGTACAT CACCATCAGC AAGGAGGAGT TATCGGCCAG CGACAGCCTG 
TCACCCGACC CCTGGAAGAG GGACGCCCGG GAAAAACTGG AGAAGCAGCA GCAGCTTCAC 
ATCGTTGACC TGCTGGACAA GGAGATCCAG GAGCTGCAGG CTAAACCCGA GCGCACGGCC 
GAGGACAACG ACCGCCTGCG CAAACTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAACGAGGA GGACGAGGAG GAGGAAGACG ATGAAGACGT GGACACCACG 
CTCATCATGC AGAGACTGGA GGCCGAGAAG AGAGCACGGC AGACTGCTGT TCCAGCAATC 
TCTGTACTAG ACTTGCTGCA GGACGAGGAG CGACGACGGA AGCAGCAGCT GGAGGAAATC 
CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGAGCCA GGAGAGAGGC TGACGAGAAG AGGAGACAGG AGGAAGAGTA CTACACCCGC 
CTGGAGGCAG AGCGGCGCCG GCAGCACGAG GAAGCTGAGC GCAAGCTGCC GACGCCAGAT 
GAGACGGGTC TGTACAGACC CCCTTTACCC CGGGACTACC AGCCCCCTTC TCCCTCAAGC 
GCACCCGCCA CTAACCACAC CACCAGCGCC CCCCCGCCTC CGCCTCAGAG AAACACCTCC 
TACCTCAAAA CCCAGGTCGC CTCCCCAGAC ACCATTTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGAGGAGGA GGATGAGGAA GATGCGGGCC TAACAGGTCA GGTTAAGCTC 
TCGGCCACCC GCAAGTCCTA CGGCGACCTT CCGCCTGCCC CAAAGCCCCA GCCGCCGCCA 
ACCGCGCGCA AGCCCCGTCC CGTCTCAGAT GGCATCTTTC TGTCCAACTC ATTCCAGCTG 
CCCGCTGGGC TCAATGCCAA CAGTACTGCT CTCAAGACAG GGCAGCCCCC TCTCCCCTTC 
ACCAAACCCA GCTTCATCCC ATCAGCCAAC TCCAAAGGAT TTAATTCATA CACTGGAAAC 
ACAACAGGAC TAGTATCAGG AGAGGTGTAT AAAGACCCGC GAGACAAGTG GACCAAAGGC 
AGCCCGGATC AAGAGAACAC AATAGGAGGA GCTCCAGAAA ACCTGACCTT CAAGGAGCGC 
CAGAGACTCT TCTCGCAGGG GAAAGAGGTC TCCAATAAAG TCAAAGCGTC CCGAAAGCTG 
ATGGAGCTGG AGAACGAACT CAACATAAAA CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352609.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208098.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X1, mRNA.

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CloneID	ODa42820
Clone ID Related Accession (Same CDS sequence)	XM_017352609.1
Accession Version	XM_017352609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCAGACTGCT 
GTTCCAGCAA TCTCTGTACT AGACTTGCTG CAGGACGAGG AGCGACGACG GAAGCAGCAG 
CTGGAGGAAA TCCGCAAGCG CGAAGCAGAA GAGCGAGCCA AACAGGAGGA GGAGAGACGC 
TGGAGAGAGG AGGAGAGAGC CAGGAGAGAG GCTGACGAGA AGAGGAGACA GGAGGAAGAG 
TACTACACCC GCCTGGAGGC AGAGCGGCGC CGGCAGCACG AGGAAGCTGA GCGCAAGCTG 
CCGACGCCAG ATGAGACGGG TCTGTACAGA CCCCCTTTAC CCCGGGACTA CCAGCCCCCT 
TCTCCCTCAA GCGCACCCGC CACTAACCAC ACCACCAGCG CCCCCCCGCC TCCGCCTCAG 
AGAAACACCT CCTACCTCAA AACCCAGGTC GCCTCCCCAG ACACCATTTA CACTGCCAAG 
TTTGTCTCGT ACAATGACGA GGAGGAGGAG GAGGATGAGG AAGATGCGGG CCTAACAGGT 
CAGGTTAAGC TCTCGGCCAC CCGCAAGTCC TACGGCGACC TTCCGCCTGC CCCAAAGCCC 
CAGCCGCCGC CAACCGCGCG CAAGCCCCGT CCCGTCTCAG ATGGCATCTT TCTGTCCAAC 
TCATTCCAGC TGCCCGCTGG GCTCAATGCC AACAGTACTG CTCTCAAGAC AGGGCAGCCC 
CCTCTCCCCT TCACCAAACC CAGCTTCATC CCATCAGCCA ACTCCAAAGG ATTTAATTCA 
TACACTGGAA ACACAACAGG ACTAGTATCA GGAGAGGTGT ATAAAGACCC GCGAGACAAG 
TGGACCAAAG GCAGCCCGGA TCAAGAGAAC ACAATAGGAG GAGCTCCAGA AAACCTGACC 
TTCAAGGAGC GCCAGAGACT CTTCTCGCAG GGGAAAGAGG TCTCCAATAA AGTCAAAGCG 
TCCCGAAAGC TGATGGAGCT GGAGAACGAA CTCAACATAA AACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208098.1
CDS	33..6020
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352609.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352609.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCAGACTGCT 
GTTCCAGCAA TCTCTGTACT AGACTTGCTG CAGGACGAGG AGCGACGACG GAAGCAGCAG 
CTGGAGGAAA TCCGCAAGCG CGAAGCAGAA GAGCGAGCCA AACAGGAGGA GGAGAGACGC 
TGGAGAGAGG AGGAGAGAGC CAGGAGAGAG GCTGACGAGA AGAGGAGACA GGAGGAAGAG 
TACTACACCC GCCTGGAGGC AGAGCGGCGC CGGCAGCACG AGGAAGCTGA GCGCAAGCTG 
CCGACGCCAG ATGAGACGGG TCTGTACAGA CCCCCTTTAC CCCGGGACTA CCAGCCCCCT 
TCTCCCTCAA GCGCACCCGC CACTAACCAC ACCACCAGCG CCCCCCCGCC TCCGCCTCAG 
AGAAACACCT CCTACCTCAA AACCCAGGTC GCCTCCCCAG ACACCATTTA CACTGCCAAG 
TTTGTCTCGT ACAATGACGA GGAGGAGGAG GAGGATGAGG AAGATGCGGG CCTAACAGGT 
CAGGTTAAGC TCTCGGCCAC CCGCAAGTCC TACGGCGACC TTCCGCCTGC CCCAAAGCCC 
CAGCCGCCGC CAACCGCGCG CAAGCCCCGT CCCGTCTCAG ATGGCATCTT TCTGTCCAAC 
TCATTCCAGC TGCCCGCTGG GCTCAATGCC AACAGTACTG CTCTCAAGAC AGGGCAGCCC 
CCTCTCCCCT TCACCAAACC CAGCTTCATC CCATCAGCCA ACTCCAAAGG ATTTAATTCA 
TACACTGGAA ACACAACAGG ACTAGTATCA GGAGAGGTGT ATAAAGACCC GCGAGACAAG 
TGGACCAAAG GCAGCCCGGA TCAAGAGAAC ACAATAGGAG GAGCTCCAGA AAACCTGACC 
TTCAAGGAGC GCCAGAGACT CTTCTCGCAG GGGAAAGAGG TCTCCAATAA AGTCAAAGCG 
TCCCGAAAGC TGATGGAGCT GGAGAACGAA CTCAACATAA AACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352610.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208099.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X2, mRNA.

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CloneID	ODa42821
Clone ID Related Accession (Same CDS sequence)	XM_017352610.1
Accession Version	XM_017352610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AGAGGAAACT GGACTTGGAT GAGAAACCAC TGGTCGTGCA GCTGAACTGG 
AATAAAGACG ACAGAGAGGG CCGCTTCGTT CTCAAGAACG AGAATGACAT TATGCCCAAG 
AGAACACTGA CAAACGGTCC TGAAAAGGAG AAAGAGGGGG TGATCCAGAA CTTCAAAAGA 
ACACTGTCCA AGAAGGAGAA GAAGAAAGAG AAGAAGAGGG AGAAGGAGGC TTTTTCCAGA 
ATCCCAGATG GAGACGATCA TGTGTTGAAC CGCGAGGATG GAGAGAACTC CAGATTAGCA 
GCTGAGGTTT ATAAAGACAT GCCGGAGACC AGTTTCACAA GGACCATTTC TAACCCCGAG 
GTGGTGATGA AGAGAAGACG ACAGCAGAAG CTGGAGAAGA GGATGCAGGA GTTTCGGAGT 
GAAGACGGTC GACCCGACTC AGGAGGCACT CTGAGGATCT ACGCTGATAG TCTGAAGCCC 
AACATTCCCT ACAAGACCAT CCTGCTCTCA ACTACTGACA TGGCTGACTT TGCTGTGGCC 
GAGGCTCTGG AGAAATATGG CCTGGAGAAG GAAAACCCTA GAGACTACTG CATCGCCCAG 
GTGATTCATG CAAATGATAA AGCAGTGAAG GAGACCATAT TAGATGACAC AGACTGTCCT 
CTGCAGATCC TCAGAGACTG GACGAGTGAT AAAGAGGCCT TAGTTTTCCA GCTGAAGAAG 
CGTCCTCCAG ATTACGCTCC AAGGAAAGGA CGTAAGCCAG ACAACCGGAT GGGGCCGCAG 
CACGGATCCA TTCCTCCTGA GAAACTGCCT TACCTGGTGG AGCTGAGCCC AGGGCGAGGG 
AGTCACTATG CCTACTACTA CCGCCATCAT GAAGATGGCT CCGACTCCAG GGACAAACCC 
AAACTCTACA GACTACAGCA CAGCGTTACT GAGGTCGGAT CTGACCGGAG TGAAGATGGA 
AACATCCAGC TGTTTGGGCC TGGGATTCTC CCTCATCACT GTGACCTTAT GCATGCGGAC 
GGCTTGGTGA CCATAACCCC AGCCAATATG GACGCAGAGA CATTTGTGGA TGGACAGCGG 
ATCGCTGACA CTACAGTTCT GCGCAGTGGG GTGACCGTCC AGTTCGGAGC AACTCACGTC 
TTCAAATTCG TAGACCCCAG TTATGACCAG AGCATGTCCA AAAGAGACCC CGGGCCCATG 
ATGAAAGGCA GGCACAAATC AGGGAGTGTC CCAGAGACGA CCTTTGACCT CCACGGGGAC 
ATTCACAGTG GCTCTGCTCT CCCTACAAGC AAGAGCTCAG GGAAGCTGGA GATGGAGCGG 
TCAGGAAACA GCAGTGAAAG AGGCCTGGTG AAGCCCATGA TTCGTGGAGA GCAGCAGGAC 
TTTAGCAAGC ATGATGGAAG GTCTCAGGAC GGCCCGGAGC TCACTCTGCC CGCCAGCATC 
GAGTTCAGGG AAAACTCCGA GGATGCCTTC CTGTCGGCCA TCATCAACTA CACCAACAGC 
TCGACAGTGC ACTTCAAGCT TTCTCCCACA TACGTGCTGT ACATGACCTG CCGTTATGTC 
ATGTCCAGCC AATACCGGCC CGACATCAGT CCCTCCGAAC GCACACACAA AGTCATCGCC 
ATTGTCAACA AGATGGTGAG CATGATGGAA GGAGTCATCC AGGAGATTGC TGAAGGTGAT 
CAGAAACAGA AGAACATCGC TGGAGCTCTG GCGTTTTGGA TGGCGAACGC TTCTGAGCTG 
CTGAACTTCA TCAAACAGGA CAAGGATCTG AGTCGCATCA CGCTGGATGC TCAGGATGTG 
CTCGCTCATC TTGTTCAGAT GGCCTTCAAG TACCTGGTGC ACTGTCTGCA GTCAGATCTC 
AATAACTACA TGCCAGCTTT CCTGGAAGAT CCCGACGAGC AAAACCCACA GAGGCCTAAG 
ATAGAGGATG TTCTGCACAC GCTGACGGGT GCCATGTCTC TGCTGCGCCG ATGCCGTGTG 
AACGCGGCGC TCACCATCCA ACTGTTTTCG CAGCTCTTCC ACTTCGTCAA CATGTGGCTC 
TTTAACAAGC TGGTGACGGA GGCCGATTCT GGCCTGTGCT CACACTACTG GGGCGCCATC 
CTTCGCCAGC AGCTCAGTCA TATTGAGGCC TGGGCTGAGA AACAAGGCTT GGAGCTGGCT 
GCAGACTGCC ACCTCAGCCG CATAGTCCAG GCGACGACCC TCCTTACTAT GGACAAGTAC 
TCCATGCAAG ATGTCCAGAA CATCAACAAC ACCTGCTTCA AGCTCAACTC TCTTCAGCTC 
AACGCTCTGC TGAGCAACTA CCACTGCGCT CCTGACGAAC CCTACATTCC TCCTGAGCTG 
ATTGATCACG TGGTGGCCGT GGCCGAAAAC ACTGCAGACG AACTGGCCCG AAGCGACGGG 
CGGGAGGTTC AGCTGGAAGA GGACCCCGAC CTTCAGCTGC CTTTCTTGCT GCCTGAGGAC 
GGCTACTCGT GCGATGTGGT GCGCAACATT CCCAACGGAT TCCAAGAGTT TCTAGAGCCA 
CTTTGCCAGA GAGGTTTCTG TAGATTGACT CCTCATCCAC GCTCGCCGGG AACTTGGACC 
ATTTATTTTG AAGGAGCCAA CTGTGACAGT CACTTCTCAG CCGACACCTC AGAACTGCCA 
ATGCGGAAGG AACCGGAAAT TGTGACCGTG ACTTTGAAGA AACATAATGG AATGGGCCTC 
AGTATTGTGG CGGCCAAGGG TGCCGGGCAG GACAAACTTG GTATTTACAT CAAATCTGTT 
GTCAAAGGAG GTGCAGCAGA CATGGATGGG CGTCTGGCTG CTGGAGACCA GTTGCTTAGC 
GTGGATGGAC GTAGTCTTGT CGGCCTCTCG CAGGAGAGAG CGGCAGAATT GATGACACGA 
ACTGGGTCTG TTGTAACGCT TGAGGTGGCC AAACAAGGAG CCATTTATCA CGGTCTGGCC 
ACACTTCTTA ACCAGCCATC CCCCATGATG CCTCGCGCGT CAGACCGTGG ACGTGGCGAC 
AAAGGAAAGC TGAGACCAAA GAGTGAGGGC TTTGAGCTCT ACAACAACTC TGTACAGAAC 
GGCTCACCTG AGAGCCCACA GGGCACGTGG GACACCTATC CTGAACCAAA GAAAGAGGAC 
AGGTTGTTGA AGAACCGAGC AGACCATCGA TCCAGCCCAA ATGTGGCCAA TCAGGCTCAG 
AGTCCAGGCG GGAAGCCTGT TTATCCGGGA GCTCCAGGAA AGATCACTTC GGTCTCGACG 
GGTAACCTCT GCCCTGATGA GGAGCCCTCT CCACCCCGTC CAGAGGCCTA TCCCATCCCC 
ACTCAGACTT ACCCTAGGGA ATACTTCACC TTCCCAGCCT CCAAATCTCA GGACAGGATG 
GGCCCTGGCC AGCCCTGGCA AAACAACGAA CAAGAGCCTC TTCCCCCTAT GGATAACCAC 
TCCAACAACA GCATGGTTAT GCAGCGGGTG GCGCGATCTC AGGAGGAGCT GTGTGACATG 
CCGGGCGGGT ACCCAGGTGA ACGTCTGCGC CAGGAAGATC TAATGCTCCT CCAGCAGCAG 
CAGCAGCGTG AAGCTGAGTA CCAGCGAGGT GTTGGAGAAC ACTGGAACCA CCAGGTGTCT 
TCCTCAGTGG AGTCCAGCAC CTCCAGCCAA GAGCACCTCA ACTTCTCCTC ATCCTCTGGC 
TCCAGGACCC AGGGCAACCA CAAGAGTGGC CCAGGCCGCT GGAAGACCCC ACTTGCACCT 
CATTCCGGCC CTGTCTCCCA ACCCTCCCGT TCAGACCTTC CTCCACCTCC ACCTCCTCCA 
CCAGCACACT ACGATTTTGA GCCGCAAACC GACCTGCCGC TTCCACCGCC ACCTTCAGCT 
ACACAACAAC AGCAGCAGGC TGCAGCCGAC CGCAAGAAAC GTGAAGACCA ACAGCGCTGG 
TATGAGAAGG AAAAAGCTCG TCTTGAAGAG GAGCGAGAGA GGAAGAGACG AGATCAGGAA 
AGGAAATTGG TGCAAATCCG AAACCCTTCG GTTTCTGGTC CAGTTATGCA CAATAACCAG 
CATGGCCCGG TGCCACCATC TCCACAAATG CCTCCCATGA CACTTCCCCA ATCATACCTT 
CCTCCAGTCC AACAACCTCC GCCACCACAA CCGGCTCGAC CCGATAAACT CTCCAGTCTT 
CCTCGCCAGC CTCCGCAAGA CACCGTAATC CGGGACCTCC TGCCGCAGCA GCAGCCGCGC 
ACCATCGAGA GACGCGACCT GCAGTACATC ACCATCAGCA AGGAGGAGTT ATCGGCCAGC 
GACAGCCTGT CACCCGACCC CTGGAAGAGG GACGCCCGGG AAAAACTGGA GAAGCAGCAG 
CAGCTTCACA TCGTTGACCT GCTGGACAAG GAGATCCAGG AGCTGCAGGC TAAACCCGAG 
CGCACGGCCG AGGACAACGA CCGCCTGCGC AAACTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTGCAGG AGTCCAAGCA GAACGAGGAG GACGAGGAGG AGGAAGACGA TGAAGACGTG 
GACACCACGC TCATCATGCA GAGACTGGAG GCCGAGAAGA GAGCACGGCA GACTGCTGTT 
CCAGCAATCT CTGTACTAGA CTTGCTGCAG GACGAGGAGC GACGACGGAA GCAGCAGCTG 
GAGGAAATCC GCAAGCGCGA AGCAGAAGAG CGAGCCAAAC AGGAGGAGGA GAGACGCTGG 
AGAGAGGAGG AGAGAGCCAG GAGAGAGGCT GACGAGAAGA GGAGACAGGA GGAAGAGTAC 
TACACCCGCC TGGAGGCAGA GCGGCGCCGG CAGCACGAGG AAGCTGAGCG CAAGCTGCCG 
ACGCCAGATG AGACGGGTCT GTACAGACCC CCTTTACCCC GGGACTACCA GCCCCCTTCT 
CCCTCAAGCG CACCCGCCAC TAACCACACC ACCAGCGCCC CCCCGCCTCC GCCTCAGAGA 
AACACCTCCT ACCTCAAAAC CCAGGTCGCC TCCCCAGACA CCATTTACAC TGCCAAGTTT 
GTCTCGTACA ATGACGAGGA GGAGGAGGAG GATGAGGAAG ATGCGGGCCT AACAGGTCAG 
GTTAAGCTCT CGGCCACCCG CAAGTCCTAC GGCGACCTTC CGCCTGCCCC AAAGCCCCAG 
CCGCCGCCAA CCGCGCGCAA GCCCCGTCCC GTCTCAGATG GCATCTTTCT GTCCAACTCA 
TTCCAGCTGC CCGCTGGGCT CAATGCCAAC AGTACTGCTC TCAAGACAGG GCAGCCCCCT 
CTCCCCTTCA CCAAACCCAG CTTCATCCCA TCAGCCAACT CCAAAGGATT TAATTCATAC 
ACTGGAAACA CAACAGGACT AGTATCAGGA GAGGTGTATA AAGACCCGCG AGACAAGTGG 
ACCAAAGGCA GCCCGGATCA AGAGAACACA ATAGGAGGAG CTCCAGAAAA CCTGACCTTC 
AAGGAGCGCC AGAGACTCTT CTCGCAGGGG AAAGAGGTCT CCAATAAAGT CAAAGCGTCC 
CGAAAGCTGA TGGAGCTGGA GAACGAACTC AACATAAAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208099.1
CDS	34..6018
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352610.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352610.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AGAGGAAACT GGACTTGGAT GAGAAACCAC TGGTCGTGCA GCTGAACTGG 
AATAAAGACG ACAGAGAGGG CCGCTTCGTT CTCAAGAACG AGAATGACAT TATGCCCAAG 
AGAACACTGA CAAACGGTCC TGAAAAGGAG AAAGAGGGGG TGATCCAGAA CTTCAAAAGA 
ACACTGTCCA AGAAGGAGAA GAAGAAAGAG AAGAAGAGGG AGAAGGAGGC TTTTTCCAGA 
ATCCCAGATG GAGACGATCA TGTGTTGAAC CGCGAGGATG GAGAGAACTC CAGATTAGCA 
GCTGAGGTTT ATAAAGACAT GCCGGAGACC AGTTTCACAA GGACCATTTC TAACCCCGAG 
GTGGTGATGA AGAGAAGACG ACAGCAGAAG CTGGAGAAGA GGATGCAGGA GTTTCGGAGT 
GAAGACGGTC GACCCGACTC AGGAGGCACT CTGAGGATCT ACGCTGATAG TCTGAAGCCC 
AACATTCCCT ACAAGACCAT CCTGCTCTCA ACTACTGACA TGGCTGACTT TGCTGTGGCC 
GAGGCTCTGG AGAAATATGG CCTGGAGAAG GAAAACCCTA GAGACTACTG CATCGCCCAG 
GTGATTCATG CAAATGATAA AGCAGTGAAG GAGACCATAT TAGATGACAC AGACTGTCCT 
CTGCAGATCC TCAGAGACTG GACGAGTGAT AAAGAGGCCT TAGTTTTCCA GCTGAAGAAG 
CGTCCTCCAG ATTACGCTCC AAGGAAAGGA CGTAAGCCAG ACAACCGGAT GGGGCCGCAG 
CACGGATCCA TTCCTCCTGA GAAACTGCCT TACCTGGTGG AGCTGAGCCC AGGGCGAGGG 
AGTCACTATG CCTACTACTA CCGCCATCAT GAAGATGGCT CCGACTCCAG GGACAAACCC 
AAACTCTACA GACTACAGCA CAGCGTTACT GAGGTCGGAT CTGACCGGAG TGAAGATGGA 
AACATCCAGC TGTTTGGGCC TGGGATTCTC CCTCATCACT GTGACCTTAT GCATGCGGAC 
GGCTTGGTGA CCATAACCCC AGCCAATATG GACGCAGAGA CATTTGTGGA TGGACAGCGG 
ATCGCTGACA CTACAGTTCT GCGCAGTGGG GTGACCGTCC AGTTCGGAGC AACTCACGTC 
TTCAAATTCG TAGACCCCAG TTATGACCAG AGCATGTCCA AAAGAGACCC CGGGCCCATG 
ATGAAAGGCA GGCACAAATC AGGGAGTGTC CCAGAGACGA CCTTTGACCT CCACGGGGAC 
ATTCACAGTG GCTCTGCTCT CCCTACAAGC AAGAGCTCAG GGAAGCTGGA GATGGAGCGG 
TCAGGAAACA GCAGTGAAAG AGGCCTGGTG AAGCCCATGA TTCGTGGAGA GCAGCAGGAC 
TTTAGCAAGC ATGATGGAAG GTCTCAGGAC GGCCCGGAGC TCACTCTGCC CGCCAGCATC 
GAGTTCAGGG AAAACTCCGA GGATGCCTTC CTGTCGGCCA TCATCAACTA CACCAACAGC 
TCGACAGTGC ACTTCAAGCT TTCTCCCACA TACGTGCTGT ACATGACCTG CCGTTATGTC 
ATGTCCAGCC AATACCGGCC CGACATCAGT CCCTCCGAAC GCACACACAA AGTCATCGCC 
ATTGTCAACA AGATGGTGAG CATGATGGAA GGAGTCATCC AGGAGATTGC TGAAGGTGAT 
CAGAAACAGA AGAACATCGC TGGAGCTCTG GCGTTTTGGA TGGCGAACGC TTCTGAGCTG 
CTGAACTTCA TCAAACAGGA CAAGGATCTG AGTCGCATCA CGCTGGATGC TCAGGATGTG 
CTCGCTCATC TTGTTCAGAT GGCCTTCAAG TACCTGGTGC ACTGTCTGCA GTCAGATCTC 
AATAACTACA TGCCAGCTTT CCTGGAAGAT CCCGACGAGC AAAACCCACA GAGGCCTAAG 
ATAGAGGATG TTCTGCACAC GCTGACGGGT GCCATGTCTC TGCTGCGCCG ATGCCGTGTG 
AACGCGGCGC TCACCATCCA ACTGTTTTCG CAGCTCTTCC ACTTCGTCAA CATGTGGCTC 
TTTAACAAGC TGGTGACGGA GGCCGATTCT GGCCTGTGCT CACACTACTG GGGCGCCATC 
CTTCGCCAGC AGCTCAGTCA TATTGAGGCC TGGGCTGAGA AACAAGGCTT GGAGCTGGCT 
GCAGACTGCC ACCTCAGCCG CATAGTCCAG GCGACGACCC TCCTTACTAT GGACAAGTAC 
TCCATGCAAG ATGTCCAGAA CATCAACAAC ACCTGCTTCA AGCTCAACTC TCTTCAGCTC 
AACGCTCTGC TGAGCAACTA CCACTGCGCT CCTGACGAAC CCTACATTCC TCCTGAGCTG 
ATTGATCACG TGGTGGCCGT GGCCGAAAAC ACTGCAGACG AACTGGCCCG AAGCGACGGG 
CGGGAGGTTC AGCTGGAAGA GGACCCCGAC CTTCAGCTGC CTTTCTTGCT GCCTGAGGAC 
GGCTACTCGT GCGATGTGGT GCGCAACATT CCCAACGGAT TCCAAGAGTT TCTAGAGCCA 
CTTTGCCAGA GAGGTTTCTG TAGATTGACT CCTCATCCAC GCTCGCCGGG AACTTGGACC 
ATTTATTTTG AAGGAGCCAA CTGTGACAGT CACTTCTCAG CCGACACCTC AGAACTGCCA 
ATGCGGAAGG AACCGGAAAT TGTGACCGTG ACTTTGAAGA AACATAATGG AATGGGCCTC 
AGTATTGTGG CGGCCAAGGG TGCCGGGCAG GACAAACTTG GTATTTACAT CAAATCTGTT 
GTCAAAGGAG GTGCAGCAGA CATGGATGGG CGTCTGGCTG CTGGAGACCA GTTGCTTAGC 
GTGGATGGAC GTAGTCTTGT CGGCCTCTCG CAGGAGAGAG CGGCAGAATT GATGACACGA 
ACTGGGTCTG TTGTAACGCT TGAGGTGGCC AAACAAGGAG CCATTTATCA CGGTCTGGCC 
ACACTTCTTA ACCAGCCATC CCCCATGATG CCTCGCGCGT CAGACCGTGG ACGTGGCGAC 
AAAGGAAAGC TGAGACCAAA GAGTGAGGGC TTTGAGCTCT ACAACAACTC TGTACAGAAC 
GGCTCACCTG AGAGCCCACA GGGCACGTGG GACACCTATC CTGAACCAAA GAAAGAGGAC 
AGGTTGTTGA AGAACCGAGC AGACCATCGA TCCAGCCCAA ATGTGGCCAA TCAGGCTCAG 
AGTCCAGGCG GGAAGCCTGT TTATCCGGGA GCTCCAGGAA AGATCACTTC GGTCTCGACG 
GGTAACCTCT GCCCTGATGA GGAGCCCTCT CCACCCCGTC CAGAGGCCTA TCCCATCCCC 
ACTCAGACTT ACCCTAGGGA ATACTTCACC TTCCCAGCCT CCAAATCTCA GGACAGGATG 
GGCCCTGGCC AGCCCTGGCA AAACAACGAA CAAGAGCCTC TTCCCCCTAT GGATAACCAC 
TCCAACAACA GCATGGTTAT GCAGCGGGTG GCGCGATCTC AGGAGGAGCT GTGTGACATG 
CCGGGCGGGT ACCCAGGTGA ACGTCTGCGC CAGGAAGATC TAATGCTCCT CCAGCAGCAG 
CAGCAGCGTG AAGCTGAGTA CCAGCGAGGT GTTGGAGAAC ACTGGAACCA CCAGGTGTCT 
TCCTCAGTGG AGTCCAGCAC CTCCAGCCAA GAGCACCTCA ACTTCTCCTC ATCCTCTGGC 
TCCAGGACCC AGGGCAACCA CAAGAGTGGC CCAGGCCGCT GGAAGACCCC ACTTGCACCT 
CATTCCGGCC CTGTCTCCCA ACCCTCCCGT TCAGACCTTC CTCCACCTCC ACCTCCTCCA 
CCAGCACACT ACGATTTTGA GCCGCAAACC GACCTGCCGC TTCCACCGCC ACCTTCAGCT 
ACACAACAAC AGCAGCAGGC TGCAGCCGAC CGCAAGAAAC GTGAAGACCA ACAGCGCTGG 
TATGAGAAGG AAAAAGCTCG TCTTGAAGAG GAGCGAGAGA GGAAGAGACG AGATCAGGAA 
AGGAAATTGG TGCAAATCCG AAACCCTTCG GTTTCTGGTC CAGTTATGCA CAATAACCAG 
CATGGCCCGG TGCCACCATC TCCACAAATG CCTCCCATGA CACTTCCCCA ATCATACCTT 
CCTCCAGTCC AACAACCTCC GCCACCACAA CCGGCTCGAC CCGATAAACT CTCCAGTCTT 
CCTCGCCAGC CTCCGCAAGA CACCGTAATC CGGGACCTCC TGCCGCAGCA GCAGCCGCGC 
ACCATCGAGA GACGCGACCT GCAGTACATC ACCATCAGCA AGGAGGAGTT ATCGGCCAGC 
GACAGCCTGT CACCCGACCC CTGGAAGAGG GACGCCCGGG AAAAACTGGA GAAGCAGCAG 
CAGCTTCACA TCGTTGACCT GCTGGACAAG GAGATCCAGG AGCTGCAGGC TAAACCCGAG 
CGCACGGCCG AGGACAACGA CCGCCTGCGC AAACTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTGCAGG AGTCCAAGCA GAACGAGGAG GACGAGGAGG AGGAAGACGA TGAAGACGTG 
GACACCACGC TCATCATGCA GAGACTGGAG GCCGAGAAGA GAGCACGGCA GACTGCTGTT 
CCAGCAATCT CTGTACTAGA CTTGCTGCAG GACGAGGAGC GACGACGGAA GCAGCAGCTG 
GAGGAAATCC GCAAGCGCGA AGCAGAAGAG CGAGCCAAAC AGGAGGAGGA GAGACGCTGG 
AGAGAGGAGG AGAGAGCCAG GAGAGAGGCT GACGAGAAGA GGAGACAGGA GGAAGAGTAC 
TACACCCGCC TGGAGGCAGA GCGGCGCCGG CAGCACGAGG AAGCTGAGCG CAAGCTGCCG 
ACGCCAGATG AGACGGGTCT GTACAGACCC CCTTTACCCC GGGACTACCA GCCCCCTTCT 
CCCTCAAGCG CACCCGCCAC TAACCACACC ACCAGCGCCC CCCCGCCTCC GCCTCAGAGA 
AACACCTCCT ACCTCAAAAC CCAGGTCGCC TCCCCAGACA CCATTTACAC TGCCAAGTTT 
GTCTCGTACA ATGACGAGGA GGAGGAGGAG GATGAGGAAG ATGCGGGCCT AACAGGTCAG 
GTTAAGCTCT CGGCCACCCG CAAGTCCTAC GGCGACCTTC CGCCTGCCCC AAAGCCCCAG 
CCGCCGCCAA CCGCGCGCAA GCCCCGTCCC GTCTCAGATG GCATCTTTCT GTCCAACTCA 
TTCCAGCTGC CCGCTGGGCT CAATGCCAAC AGTACTGCTC TCAAGACAGG GCAGCCCCCT 
CTCCCCTTCA CCAAACCCAG CTTCATCCCA TCAGCCAACT CCAAAGGATT TAATTCATAC 
ACTGGAAACA CAACAGGACT AGTATCAGGA GAGGTGTATA AAGACCCGCG AGACAAGTGG 
ACCAAAGGCA GCCCGGATCA AGAGAACACA ATAGGAGGAG CTCCAGAAAA CCTGACCTTC 
AAGGAGCGCC AGAGACTCTT CTCGCAGGGG AAAGAGGTCT CCAATAAAGT CAAAGCGTCC 
CGAAAGCTGA TGGAGCTGGA GAACGAACTC AACATAAAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352611.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208100.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X3, mRNA.

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CloneID	ODa42822
Clone ID Related Accession (Same CDS sequence)	XM_017352611.1
Accession Version	XM_017352611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCAGACTGCT 
GTTCCAGCAA TCTCTGTACT AGACTTGCTG CAGGACGAGG AGCGACGACG GAAGCAGCAG 
CTGGAGGAAA TCCGCAAGCG CGAAGCAGAA GAGCGAGCCA AACAGGAGGA GGAGAGACGC 
TGGAGAGAGG AGGAGAGAGC CAGGAGAGAG GCTGACGAGA AGAGGAGACA GGAGGAAGAG 
TACTACACCC GCCTGGAGGC AGAGCGGCGC CGGCAGCACG AGGAAGCTGA GCGCAAGCTG 
CCGACGCCAG ATGAGACGGG TCTGTACAGA CCCCCTTTAC CCCGGGACTA CCAGCCCCCT 
TCTCCCTCAA GCGCACCCGC CACTAACCAC ACCACCAGCG CCCCCCCGCC TCCGCCTCAG 
AGAAACACCT CCTACCTCAA AACCCAGGTC GCCTCCCCAG ACACCATTTA CACTGCCAAG 
TTTGTCTCGT ACAATGACGA GGAGGAGGAG GAGGATGAGG AAGATGCGGG CCTAACAGGT 
CAGGTTAAGC TCTCGGCCAC CCGCAAGTCC TACGGCGACC TTCCGCCTGC CCCAAAGCCC 
CAGCCGCCGC CAACCGCGCG CAAGCCCCGT CCCGTCTCAG ATGGCATCTT TCTGTCCAAC 
TCATTCCAGC TGCCCGCTGG GCTCAATGCC AACAGTACTG CTCTCAAGAC AGGGCAGCCC 
CCTCTCCCCT TCACCAAACC CAGCTTCATC CCATCAGCCA ACTCCAAAGG ATTTAATTCA 
TACACTGGAA ACACAACAGG ACTAGTATCA GGAGAGGTGT ATAAAGACCC GCGAGACAAG 
TGGACCAAAG GCCCGGATCA AGAGAACACA ATAGGAGGAG CTCCAGAAAA CCTGACCTTC 
AAGGAGCGCC AGAGACTCTT CTCGCAGGGG AAAGAGGTCT CCAATAAAGT CAAAGCGTCC 
CGAAAGCTGA TGGAGCTGGA GAACGAACTC AACATAAAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208100.1
CDS	36..6020
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352611.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352611.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCAGACTGCT 
GTTCCAGCAA TCTCTGTACT AGACTTGCTG CAGGACGAGG AGCGACGACG GAAGCAGCAG 
CTGGAGGAAA TCCGCAAGCG CGAAGCAGAA GAGCGAGCCA AACAGGAGGA GGAGAGACGC 
TGGAGAGAGG AGGAGAGAGC CAGGAGAGAG GCTGACGAGA AGAGGAGACA GGAGGAAGAG 
TACTACACCC GCCTGGAGGC AGAGCGGCGC CGGCAGCACG AGGAAGCTGA GCGCAAGCTG 
CCGACGCCAG ATGAGACGGG TCTGTACAGA CCCCCTTTAC CCCGGGACTA CCAGCCCCCT 
TCTCCCTCAA GCGCACCCGC CACTAACCAC ACCACCAGCG CCCCCCCGCC TCCGCCTCAG 
AGAAACACCT CCTACCTCAA AACCCAGGTC GCCTCCCCAG ACACCATTTA CACTGCCAAG 
TTTGTCTCGT ACAATGACGA GGAGGAGGAG GAGGATGAGG AAGATGCGGG CCTAACAGGT 
CAGGTTAAGC TCTCGGCCAC CCGCAAGTCC TACGGCGACC TTCCGCCTGC CCCAAAGCCC 
CAGCCGCCGC CAACCGCGCG CAAGCCCCGT CCCGTCTCAG ATGGCATCTT TCTGTCCAAC 
TCATTCCAGC TGCCCGCTGG GCTCAATGCC AACAGTACTG CTCTCAAGAC AGGGCAGCCC 
CCTCTCCCCT TCACCAAACC CAGCTTCATC CCATCAGCCA ACTCCAAAGG ATTTAATTCA 
TACACTGGAA ACACAACAGG ACTAGTATCA GGAGAGGTGT ATAAAGACCC GCGAGACAAG 
TGGACCAAAG GCCCGGATCA AGAGAACACA ATAGGAGGAG CTCCAGAAAA CCTGACCTTC 
AAGGAGCGCC AGAGACTCTT CTCGCAGGGG AAAGAGGTCT CCAATAAAGT CAAAGCGTCC 
CGAAAGCTGA TGGAGCTGGA GAACGAACTC AACATAAAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352612.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208101.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X4, mRNA.

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CloneID	ODa42823
Clone ID Related Accession (Same CDS sequence)	XM_017352612.1
Accession Version	XM_017352612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGAAACA GAAGAACATC 
GCTGGAGCTC TGGCGTTTTG GATGGCGAAC GCTTCTGAGC TGCTGAACTT CATCAAACAG 
GACAAGGATC TGAGTCGCAT CACGCTGGAT GCTCAGGATG TGCTCGCTCA TCTTGTTCAG 
ATGGCCTTCA AGTACCTGGT GCACTGTCTG CAGTCAGATC TCAATAACTA CATGCCAGCT 
TTCCTGGAAG ATCCCGACGA GCAAAACCCA CAGAGGCCTA AGATAGAGGA TGTTCTGCAC 
ACGCTGACGG GTGCCATGTC TCTGCTGCGC CGATGCCGTG TGAACGCGGC GCTCACCATC 
CAACTGTTTT CGCAGCTCTT CCACTTCGTC AACATGTGGC TCTTTAACAA GCTGGTGACG 
GAGGCCGATT CTGGCCTGTG CTCACACTAC TGGGGCGCCA TCCTTCGCCA GCAGCTCAGT 
CATATTGAGG CCTGGGCTGA GAAACAAGGC TTGGAGCTGG CTGCAGACTG CCACCTCAGC 
CGCATAGTCC AGGCGACGAC CCTCCTTACT ATGGACAAGT ACTCCATGCA AGATGTCCAG 
AACATCAACA ACACCTGCTT CAAGCTCAAC TCTCTTCAGC TCAACGCTCT GCTGAGCAAC 
TACCACTGCG CTCCTGACGA ACCCTACATT CCTCCTGAGC TGATTGATCA CGTGGTGGCC 
GTGGCCGAAA ACACTGCAGA CGAACTGGCC CGAAGCGACG GGCGGGAGGT TCAGCTGGAA 
GAGGACCCCG ACCTTCAGCT GCCTTTCTTG CTGCCTGAGG ACGGCTACTC GTGCGATGTG 
GTGCGCAACA TTCCCAACGG ATTCCAAGAG TTTCTAGAGC CACTTTGCCA GAGAGGTTTC 
TGTAGATTGA CTCCTCATCC ACGCTCGCCG GGAACTTGGA CCATTTATTT TGAAGGAGCC 
AACTGTGACA GTCACTTCTC AGCCGACACC TCAGAACTGC CAATGCGGAA GGAACCGGAA 
ATTGTGACCG TGACTTTGAA GAAACATAAT GGAATGGGCC TCAGTATTGT GGCGGCCAAG 
GGTGCCGGGC AGGACAAACT TGGTATTTAC ATCAAATCTG TTGTCAAAGG AGGTGCAGCA 
GACATGGATG GGCGTCTGGC TGCTGGAGAC CAGTTGCTTA GCGTGGATGG ACGTAGTCTT 
GTCGGCCTCT CGCAGGAGAG AGCGGCAGAA TTGATGACAC GAACTGGGTC TGTTGTAACG 
CTTGAGGTGG CCAAACAAGG AGCCATTTAT CACGGTCTGG CCACACTTCT TAACCAGCCA 
TCCCCCATGA TGCCTCGCGC GTCAGACCGT GGACGTGGCG ACAAAGGAAA GCTGAGACCA 
AAGAGTGAGG GCTTTGAGCT CTACAACAAC TCTGTACAGA ACGGCTCACC TGAGAGCCCA 
CAGGGCACGT GGGACACCTA TCCTGAACCA AAGAAAGAGG ACAGGTTGTT GAAGAACCGA 
GCAGACCATC GATCCAGCCC AAATGTGGCC AATCAGGCTC AGAGTCCAGG CGGGAAGCCT 
GTTTATCCGG GAGCTCCAGG AAAGATCACT TCGGTCTCGA CGGGTAACCT CTGCCCTGAT 
GAGGAGCCCT CTCCACCCCG TCCAGAGGCC TATCCCATCC CCACTCAGAC TTACCCTAGG 
GAATACTTCA CCTTCCCAGC CTCCAAATCT CAGGACAGGA TGGGCCCTGG CCAGCCCTGG 
CAAAACAACG AACAAGAGCC TCTTCCCCCT ATGGATAACC ACTCCAACAA CAGCATGGTT 
ATGCAGCGGG TGGCGCGATC TCAGGAGGAG CTGTGTGACA TGCCGGGCGG GTACCCAGGT 
GAACGTCTGC GCCAGGAAGA TCTAATGCTC CTCCAGCAGC AGCAGCAGCG TGAAGCTGAG 
TACCAGCGAG GTGTTGGAGA ACACTGGAAC CACCAGGTGT CTTCCTCAGT GGAGTCCAGC 
ACCTCCAGCC AAGAGCACCT CAACTTCTCC TCATCCTCTG GCTCCAGGAC CCAGGGCAAC 
CACAAGAGTG GCCCAGGCCG CTGGAAGACC CCACTTGCAC CTCATTCCGG CCCTGTCTCC 
CAACCCTCCC GTTCAGACCT TCCTCCACCT CCACCTCCTC CACCAGCACA CTACGATTTT 
GAGCCGCAAA CCGACCTGCC GCTTCCACCG CCACCTTCAG CTACACAACA ACAGCAGCAG 
GCTGCAGCCG ACCGCAAGAA ACGTGAAGAC CAACAGCGCT GGTATGAGAA GGAAAAAGCT 
CGTCTTGAAG AGGAGCGAGA GAGGAAGAGA CGAGATCAGG AAAGGAAATT GGTGCAAATC 
CGAAACCCTT CGGTTTCTGG TCCAGTTATG CACAATAACC AGCATGGCCC GGTGCCACCA 
TCTCCACAAA TGCCTCCCAT GACACTTCCC CAATCATACC TTCCTCCAGT CCAACAACCT 
CCGCCACCAC AACCGGCTCG ACCCGATAAA CTCTCCAGTC TTCCTCGCCA GCCTCCGCAA 
GACACCGTAA TCCGGGACCT CCTGCCGCAG CAGCAGCCGC GCACCATCGA GAGACGCGAC 
CTGCAGTACA TCACCATCAG CAAGGAGGAG TTATCGGCCA GCGACAGCCT GTCACCCGAC 
CCCTGGAAGA GGGACGCCCG GGAAAAACTG GAGAAGCAGC AGCAGCTTCA CATCGTTGAC 
CTGCTGGACA AGGAGATCCA GGAGCTGCAG GCTAAACCCG AGCGCACGGC CGAGGACAAC 
GACCGCCTGC GCAAACTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTGCA GGAGTCCAAG 
CAGAACGAGG AGGACGAGGA GGAGGAAGAC GATGAAGACG TGGACACCAC GCTCATCATG 
CAGAGACTGG AGGCCGAGAA GAGAGCACGG CAGACTGCTG TTCCAGCAAT CTCTGTACTA 
GACTTGCTGC AGGACGAGGA GCGACGACGG AAGCAGCAGC TGGAGGAAAT CCGCAAGCGC 
GAAGCAGAAG AGCGAGCCAA ACAGGAGGAG GAGAGACGCT GGAGAGAGGA GGAGAGAGCC 
AGGAGAGAGG CTGACGAGAA GAGGAGACAG GAGGAAGAGT ACTACACCCG CCTGGAGGCA 
GAGCGGCGCC GGCAGCACGA GGAAGCTGAG CGCAAGCTGC CGACGCCAGA TGAGACGGGT 
CTGTACAGAC CCCCTTTACC CCGGGACTAC CAGCCCCCTT CTCCCTCAAG CGCACCCGCC 
ACTAACCACA CCACCAGCGC CCCCCCGCCT CCGCCTCAGA GAAACACCTC CTACCTCAAA 
ACCCAGGTCG CCTCCCCAGA CACCATTTAC ACTGCCAAGT TTGTCTCGTA CAATGACGAG 
GAGGAGGAGG AGGATGAGGA AGATGCGGGC CTAACAGGTC AGGTTAAGCT CTCGGCCACC 
CGCAAGTCCT ACGGCGACCT TCCGCCTGCC CCAAAGCCCC AGCCGCCGCC AACCGCGCGC 
AAGCCCCGTC CCGTCTCAGA TGGCATCTTT CTGTCCAACT CATTCCAGCT GCCCGCTGGG 
CTCAATGCCA ACAGTACTGC TCTCAAGACA GGGCAGCCCC CTCTCCCCTT CACCAAACCC 
AGCTTCATCC CATCAGCCAA CTCCAAAGGA TTTAATTCAT ACACTGGAAA CACAACAGGA 
CTAGTATCAG GAGAGGTGTA TAAAGACCCG CGAGACAAGT GGACCAAAGG CAGCCCGGAT 
CAAGAGAACA CAATAGGAGG AGCTCCAGAA AACCTGACCT TCAAGGAGCG CCAGAGACTC 
TTCTCGCAGG GGAAAGAGGT CTCCAATAAA GTCAAAGCGT CCCGAAAGCT GATGGAGCTG 
GAGAACGAAC TCAACATAAA ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208101.1
CDS	32..5998
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352612.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352612.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGAAACA GAAGAACATC 
GCTGGAGCTC TGGCGTTTTG GATGGCGAAC GCTTCTGAGC TGCTGAACTT CATCAAACAG 
GACAAGGATC TGAGTCGCAT CACGCTGGAT GCTCAGGATG TGCTCGCTCA TCTTGTTCAG 
ATGGCCTTCA AGTACCTGGT GCACTGTCTG CAGTCAGATC TCAATAACTA CATGCCAGCT 
TTCCTGGAAG ATCCCGACGA GCAAAACCCA CAGAGGCCTA AGATAGAGGA TGTTCTGCAC 
ACGCTGACGG GTGCCATGTC TCTGCTGCGC CGATGCCGTG TGAACGCGGC GCTCACCATC 
CAACTGTTTT CGCAGCTCTT CCACTTCGTC AACATGTGGC TCTTTAACAA GCTGGTGACG 
GAGGCCGATT CTGGCCTGTG CTCACACTAC TGGGGCGCCA TCCTTCGCCA GCAGCTCAGT 
CATATTGAGG CCTGGGCTGA GAAACAAGGC TTGGAGCTGG CTGCAGACTG CCACCTCAGC 
CGCATAGTCC AGGCGACGAC CCTCCTTACT ATGGACAAGT ACTCCATGCA AGATGTCCAG 
AACATCAACA ACACCTGCTT CAAGCTCAAC TCTCTTCAGC TCAACGCTCT GCTGAGCAAC 
TACCACTGCG CTCCTGACGA ACCCTACATT CCTCCTGAGC TGATTGATCA CGTGGTGGCC 
GTGGCCGAAA ACACTGCAGA CGAACTGGCC CGAAGCGACG GGCGGGAGGT TCAGCTGGAA 
GAGGACCCCG ACCTTCAGCT GCCTTTCTTG CTGCCTGAGG ACGGCTACTC GTGCGATGTG 
GTGCGCAACA TTCCCAACGG ATTCCAAGAG TTTCTAGAGC CACTTTGCCA GAGAGGTTTC 
TGTAGATTGA CTCCTCATCC ACGCTCGCCG GGAACTTGGA CCATTTATTT TGAAGGAGCC 
AACTGTGACA GTCACTTCTC AGCCGACACC TCAGAACTGC CAATGCGGAA GGAACCGGAA 
ATTGTGACCG TGACTTTGAA GAAACATAAT GGAATGGGCC TCAGTATTGT GGCGGCCAAG 
GGTGCCGGGC AGGACAAACT TGGTATTTAC ATCAAATCTG TTGTCAAAGG AGGTGCAGCA 
GACATGGATG GGCGTCTGGC TGCTGGAGAC CAGTTGCTTA GCGTGGATGG ACGTAGTCTT 
GTCGGCCTCT CGCAGGAGAG AGCGGCAGAA TTGATGACAC GAACTGGGTC TGTTGTAACG 
CTTGAGGTGG CCAAACAAGG AGCCATTTAT CACGGTCTGG CCACACTTCT TAACCAGCCA 
TCCCCCATGA TGCCTCGCGC GTCAGACCGT GGACGTGGCG ACAAAGGAAA GCTGAGACCA 
AAGAGTGAGG GCTTTGAGCT CTACAACAAC TCTGTACAGA ACGGCTCACC TGAGAGCCCA 
CAGGGCACGT GGGACACCTA TCCTGAACCA AAGAAAGAGG ACAGGTTGTT GAAGAACCGA 
GCAGACCATC GATCCAGCCC AAATGTGGCC AATCAGGCTC AGAGTCCAGG CGGGAAGCCT 
GTTTATCCGG GAGCTCCAGG AAAGATCACT TCGGTCTCGA CGGGTAACCT CTGCCCTGAT 
GAGGAGCCCT CTCCACCCCG TCCAGAGGCC TATCCCATCC CCACTCAGAC TTACCCTAGG 
GAATACTTCA CCTTCCCAGC CTCCAAATCT CAGGACAGGA TGGGCCCTGG CCAGCCCTGG 
CAAAACAACG AACAAGAGCC TCTTCCCCCT ATGGATAACC ACTCCAACAA CAGCATGGTT 
ATGCAGCGGG TGGCGCGATC TCAGGAGGAG CTGTGTGACA TGCCGGGCGG GTACCCAGGT 
GAACGTCTGC GCCAGGAAGA TCTAATGCTC CTCCAGCAGC AGCAGCAGCG TGAAGCTGAG 
TACCAGCGAG GTGTTGGAGA ACACTGGAAC CACCAGGTGT CTTCCTCAGT GGAGTCCAGC 
ACCTCCAGCC AAGAGCACCT CAACTTCTCC TCATCCTCTG GCTCCAGGAC CCAGGGCAAC 
CACAAGAGTG GCCCAGGCCG CTGGAAGACC CCACTTGCAC CTCATTCCGG CCCTGTCTCC 
CAACCCTCCC GTTCAGACCT TCCTCCACCT CCACCTCCTC CACCAGCACA CTACGATTTT 
GAGCCGCAAA CCGACCTGCC GCTTCCACCG CCACCTTCAG CTACACAACA ACAGCAGCAG 
GCTGCAGCCG ACCGCAAGAA ACGTGAAGAC CAACAGCGCT GGTATGAGAA GGAAAAAGCT 
CGTCTTGAAG AGGAGCGAGA GAGGAAGAGA CGAGATCAGG AAAGGAAATT GGTGCAAATC 
CGAAACCCTT CGGTTTCTGG TCCAGTTATG CACAATAACC AGCATGGCCC GGTGCCACCA 
TCTCCACAAA TGCCTCCCAT GACACTTCCC CAATCATACC TTCCTCCAGT CCAACAACCT 
CCGCCACCAC AACCGGCTCG ACCCGATAAA CTCTCCAGTC TTCCTCGCCA GCCTCCGCAA 
GACACCGTAA TCCGGGACCT CCTGCCGCAG CAGCAGCCGC GCACCATCGA GAGACGCGAC 
CTGCAGTACA TCACCATCAG CAAGGAGGAG TTATCGGCCA GCGACAGCCT GTCACCCGAC 
CCCTGGAAGA GGGACGCCCG GGAAAAACTG GAGAAGCAGC AGCAGCTTCA CATCGTTGAC 
CTGCTGGACA AGGAGATCCA GGAGCTGCAG GCTAAACCCG AGCGCACGGC CGAGGACAAC 
GACCGCCTGC GCAAACTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTGCA GGAGTCCAAG 
CAGAACGAGG AGGACGAGGA GGAGGAAGAC GATGAAGACG TGGACACCAC GCTCATCATG 
CAGAGACTGG AGGCCGAGAA GAGAGCACGG CAGACTGCTG TTCCAGCAAT CTCTGTACTA 
GACTTGCTGC AGGACGAGGA GCGACGACGG AAGCAGCAGC TGGAGGAAAT CCGCAAGCGC 
GAAGCAGAAG AGCGAGCCAA ACAGGAGGAG GAGAGACGCT GGAGAGAGGA GGAGAGAGCC 
AGGAGAGAGG CTGACGAGAA GAGGAGACAG GAGGAAGAGT ACTACACCCG CCTGGAGGCA 
GAGCGGCGCC GGCAGCACGA GGAAGCTGAG CGCAAGCTGC CGACGCCAGA TGAGACGGGT 
CTGTACAGAC CCCCTTTACC CCGGGACTAC CAGCCCCCTT CTCCCTCAAG CGCACCCGCC 
ACTAACCACA CCACCAGCGC CCCCCCGCCT CCGCCTCAGA GAAACACCTC CTACCTCAAA 
ACCCAGGTCG CCTCCCCAGA CACCATTTAC ACTGCCAAGT TTGTCTCGTA CAATGACGAG 
GAGGAGGAGG AGGATGAGGA AGATGCGGGC CTAACAGGTC AGGTTAAGCT CTCGGCCACC 
CGCAAGTCCT ACGGCGACCT TCCGCCTGCC CCAAAGCCCC AGCCGCCGCC AACCGCGCGC 
AAGCCCCGTC CCGTCTCAGA TGGCATCTTT CTGTCCAACT CATTCCAGCT GCCCGCTGGG 
CTCAATGCCA ACAGTACTGC TCTCAAGACA GGGCAGCCCC CTCTCCCCTT CACCAAACCC 
AGCTTCATCC CATCAGCCAA CTCCAAAGGA TTTAATTCAT ACACTGGAAA CACAACAGGA 
CTAGTATCAG GAGAGGTGTA TAAAGACCCG CGAGACAAGT GGACCAAAGG CAGCCCGGAT 
CAAGAGAACA CAATAGGAGG AGCTCCAGAA AACCTGACCT TCAAGGAGCG CCAGAGACTC 
TTCTCGCAGG GGAAAGAGGT CTCCAATAAA GTCAAAGCGT CCCGAAAGCT GATGGAGCTG 
GAGAACGAAC TCAACATAAA ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352613.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208102.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X5, mRNA.

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CloneID	ODa42824
Clone ID Related Accession (Same CDS sequence)	XM_017352613.1
Accession Version	XM_017352613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCTGCAGGAC 
GAGGAGCGAC GACGGAAGCA GCAGCTGGAG GAAATCCGCA AGCGCGAAGC AGAAGAGCGA 
GCCAAACAGG AGGAGGAGAG ACGCTGGAGA GAGGAGGAGA GAGCCAGGAG AGAGGCTGAC 
GAGAAGAGGA GACAGGAGGA AGAGTACTAC ACCCGCCTGG AGGCAGAGCG GCGCCGGCAG 
CACGAGGAAG CTGAGCGCAA GCTGCCGACG CCAGATGAGA CGGGTCTGTA CAGACCCCCT 
TTACCCCGGG ACTACCAGCC CCCTTCTCCC TCAAGCGCAC CCGCCACTAA CCACACCACC 
AGCGCCCCCC CGCCTCCGCC TCAGAGAAAC ACCTCCTACC TCAAAACCCA GGTCGCCTCC 
CCAGACACCA TTTACACTGC CAAGTTTGTC TCGTACAATG ACGAGGAGGA GGAGGAGGAT 
GAGGAAGATG CGGGCCTAAC AGGTCAGGTT AAGCTCTCGG CCACCCGCAA GTCCTACGGC 
GACCTTCCGC CTGCCCCAAA GCCCCAGCCG CCGCCAACCG CGCGCAAGCC CCGTCCCGTC 
TCAGATGGCA TCTTTCTGTC CAACTCATTC CAGCTGCCCG CTGGGCTCAA TGCCAACAGT 
ACTGCTCTCA AGACAGGGCA GCCCCCTCTC CCCTTCACCA AACCCAGCTT CATCCCATCA 
GCCAACTCCA AAGGATTTAA TTCATACACT GGAAACACAA CAGGACTAGT ATCAGGAGAG 
GTGTATAAAG ACCCGCGAGA CAAGTGGACC AAAGGCAGCC CGGATCAAGA GAACACAATA 
GGAGGAGCTC CAGAAAACCT GACCTTCAAG GAGCGCCAGA GACTCTTCTC GCAGGGGAAA 
GAGGTCTCCA ATAAAGTCAA AGCGTCCCGA AAGCTGATGG AGCTGGAGAA CGAACTCAAC 
ATAAAACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208102.1
CDS	30..5981
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352613.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352613.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCTGCAGGAC 
GAGGAGCGAC GACGGAAGCA GCAGCTGGAG GAAATCCGCA AGCGCGAAGC AGAAGAGCGA 
GCCAAACAGG AGGAGGAGAG ACGCTGGAGA GAGGAGGAGA GAGCCAGGAG AGAGGCTGAC 
GAGAAGAGGA GACAGGAGGA AGAGTACTAC ACCCGCCTGG AGGCAGAGCG GCGCCGGCAG 
CACGAGGAAG CTGAGCGCAA GCTGCCGACG CCAGATGAGA CGGGTCTGTA CAGACCCCCT 
TTACCCCGGG ACTACCAGCC CCCTTCTCCC TCAAGCGCAC CCGCCACTAA CCACACCACC 
AGCGCCCCCC CGCCTCCGCC TCAGAGAAAC ACCTCCTACC TCAAAACCCA GGTCGCCTCC 
CCAGACACCA TTTACACTGC CAAGTTTGTC TCGTACAATG ACGAGGAGGA GGAGGAGGAT 
GAGGAAGATG CGGGCCTAAC AGGTCAGGTT AAGCTCTCGG CCACCCGCAA GTCCTACGGC 
GACCTTCCGC CTGCCCCAAA GCCCCAGCCG CCGCCAACCG CGCGCAAGCC CCGTCCCGTC 
TCAGATGGCA TCTTTCTGTC CAACTCATTC CAGCTGCCCG CTGGGCTCAA TGCCAACAGT 
ACTGCTCTCA AGACAGGGCA GCCCCCTCTC CCCTTCACCA AACCCAGCTT CATCCCATCA 
GCCAACTCCA AAGGATTTAA TTCATACACT GGAAACACAA CAGGACTAGT ATCAGGAGAG 
GTGTATAAAG ACCCGCGAGA CAAGTGGACC AAAGGCAGCC CGGATCAAGA GAACACAATA 
GGAGGAGCTC CAGAAAACCT GACCTTCAAG GAGCGCCAGA GACTCTTCTC GCAGGGGAAA 
GAGGTCTCCA ATAAAGTCAA AGCGTCCCGA AAGCTGATGG AGCTGGAGAA CGAACTCAAC 
ATAAAACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352614.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208103.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X6, mRNA.

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CloneID	ODa42825
Clone ID Related Accession (Same CDS sequence)	XM_017352614.1
Accession Version	XM_017352614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGATGGC 
TCCGACTCCA GGGACAAACC CAAACTCTAC AGACTACAGC ACAGCGTTAC TGAGGTCGGA 
TCTGACCGGA GTGAAGATGG AAACATCCAG CTGTTTGGGC CTGGGATTCT CCCTCATCAC 
TGTGACCTTA TGCATGCGGA CGGCTTGGTG ACCATAACCC CAGCCAATAT GGACGCAGAG 
ACATTTGTGG ATGGACAGCG GATCGCTGAC ACTACAGTTC TGCGCAGTGG GGTGACCGTC 
CAGTTCGGAG CAACTCACGT CTTCAAATTC GTAGACCCCA GTTATGACCA GAGCATGTCC 
AAAAGAGACC CCGGGCCCAT GATGAAAGGC AGGCACAAAT CAGGGAGTGT CCCAGAGACG 
ACCTTTGACC TCCACGGGGA CATTCACAGT GGCTCTGCTC TCCCTACAAG CAAGAGCTCA 
GGGAAGCTGG AGATGGAGCG GTCAGGAAAC AGCAGTGAAA GAGGCCTGGT GAAGCCCATG 
ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG CATGATGGAA GGTCTCAGGA CGGCCCGGAG 
CTCACTCTGC CCGCCAGCAT CGAGTTCAGG GAAAACTCCG AGGATGCCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCGACAGTG CACTTCAAGC TTTCTCCCAC ATACGTGCTG 
TACATGACCT GCCGTTATGT CATGTCCAGC CAATACCGGC CCGACATCAG TCCCTCCGAA 
CGCACACACA AAGTCATCGC CATTGTCAAC AAGATGGTGA GCATGATGGA AGGAGTCATC 
CAGGAGATTG CTGAAGGTGA TCAGAAACAG AAGAACATCG CTGGAGCTCT GGCGTTTTGG 
ATGGCGAACG CTTCTGAGCT GCTGAACTTC ATCAAACAGG ACAAGGATCT GAGTCGCATC 
ACGCTGGATG CTCAGGATGT GCTCGCTCAT CTTGTTCAGA TGGCCTTCAA GTACCTGGTG 
CACTGTCTGC AGTCAGATCT CAATAACTAC ATGCCAGCTT TCCTGGAAGA TCCCGACGAG 
CAAAACCCAC AGAGGCCTAA GATAGAGGAT GTTCTGCACA CGCTGACGGG TGCCATGTCT 
CTGCTGCGCC GATGCCGTGT GAACGCGGCG CTCACCATCC AACTGTTTTC GCAGCTCTTC 
CACTTCGTCA ACATGTGGCT CTTTAACAAG CTGGTGACGG AGGCCGATTC TGGCCTGTGC 
TCACACTACT GGGGCGCCAT CCTTCGCCAG CAGCTCAGTC ATATTGAGGC CTGGGCTGAG 
AAACAAGGCT TGGAGCTGGC TGCAGACTGC CACCTCAGCC GCATAGTCCA GGCGACGACC 
CTCCTTACTA TGGACAAGTA CTCCATGCAA GATGTCCAGA ACATCAACAA CACCTGCTTC 
AAGCTCAACT CTCTTCAGCT CAACGCTCTG CTGAGCAACT ACCACTGCGC TCCTGACGAA 
CCCTACATTC CTCCTGAGCT GATTGATCAC GTGGTGGCCG TGGCCGAAAA CACTGCAGAC 
GAACTGGCCC GAAGCGACGG GCGGGAGGTT CAGCTGGAAG AGGACCCCGA CCTTCAGCTG 
CCTTTCTTGC TGCCTGAGGA CGGCTACTCG TGCGATGTGG TGCGCAACAT TCCCAACGGA 
TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG AGAGGTTTCT GTAGATTGAC TCCTCATCCA 
CGCTCGCCGG GAACTTGGAC CATTTATTTT GAAGGAGCCA ACTGTGACAG TCACTTCTCA 
GCCGACACCT CAGAACTGCC AATGCGGAAG GAACCGGAAA TTGTGACCGT GACTTTGAAG 
AAACATAATG GAATGGGCCT CAGTATTGTG GCGGCCAAGG GTGCCGGGCA GGACAAACTT 
GGTATTTACA TCAAATCTGT TGTCAAAGGA GGTGCAGCAG ACATGGATGG GCGTCTGGCT 
GCTGGAGACC AGTTGCTTAG CGTGGATGGA CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA 
GCGGCAGAAT TGATGACACG AACTGGGTCT GTTGTAACGC TTGAGGTGGC CAAACAAGGA 
GCCATTTATC ACGGTCTGGC CACACTTCTT AACCAGCCAT CCCCCATGAT GCCTCGCGCG 
TCAGACCGTG GACGTGGCGA CAAAGGAAAG CTGAGACCAA AGAGTGAGGG CTTTGAGCTC 
TACAACAACT CTGTACAGAA CGGCTCACCT GAGAGCCCAC AGGGCACGTG GGACACCTAT 
CCTGAACCAA AGAAAGAGGA CAGGTTGTTG AAGAACCGAG CAGACCATCG ATCCAGCCCA 
AATGTGGCCA ATCAGGCTCA GAGTCCAGGC GGGAAGCCTG TTTATCCGGG AGCTCCAGGA 
AAGATCACTT CGGTCTCGAC GGGTAACCTC TGCCCTGATG AGGAGCCCTC TCCACCCCGT 
CCAGAGGCCT ATCCCATCCC CACTCAGACT TACCCTAGGG AATACTTCAC CTTCCCAGCC 
TCCAAATCTC AGGACAGGAT GGGCCCTGGC CAGCCCTGGC AAAACAACGA ACAAGAGCCT 
CTTCCCCCTA TGGATAACCA CTCCAACAAC AGCATGGTTA TGCAGCGGGT GGCGCGATCT 
CAGGAGGAGC TGTGTGACAT GCCGGGCGGG TACCCAGGTG AACGTCTGCG CCAGGAAGAT 
CTAATGCTCC TCCAGCAGCA GCAGCAGCGT GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA 
CACTGGAACC ACCAGGTGTC TTCCTCAGTG GAGTCCAGCA CCTCCAGCCA AGAGCACCTC 
AACTTCTCCT CATCCTCTGG CTCCAGGACC CAGGGCAACC ACAAGAGTGG CCCAGGCCGC 
TGGAAGACCC CACTTGCACC TCATTCCGGC CCTGTCTCCC AACCCTCCCG TTCAGACCTT 
CCTCCACCTC CACCTCCTCC ACCAGCACAC TACGATTTTG AGCCGCAAAC CGACCTGCCG 
CTTCCACCGC CACCTTCAGC TACACAACAA CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA 
CGTGAAGACC AACAGCGCTG GTATGAGAAG GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG 
AGGAAGAGAC GAGATCAGGA AAGGAAATTG GTGCAAATCC GAAACCCTTC GGTTTCTGGT 
CCAGTTATGC ACAATAACCA GCATGGCCCG GTGCCACCAT CTCCACAAAT GCCTCCCATG 
ACACTTCCCC AATCATACCT TCCTCCAGTC CAACAACCTC CGCCACCACA ACCGGCTCGA 
CCCGATAAAC TCTCCAGTCT TCCTCGCCAG CCTCCGCAAG ACACCGTAAT CCGGGACCTC 
CTGCCGCAGC AGCAGCCGCG CACCATCGAG AGACGCGACC TGCAGTACAT CACCATCAGC 
AAGGAGGAGT TATCGGCCAG CGACAGCCTG TCACCCGACC CCTGGAAGAG GGACGCCCGG 
GAAAAACTGG AGAAGCAGCA GCAGCTTCAC ATCGTTGACC TGCTGGACAA GGAGATCCAG 
GAGCTGCAGG CTAAACCCGA GCGCACGGCC GAGGACAACG ACCGCCTGCG CAAACTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAACGAGGA GGACGAGGAG 
GAGGAAGACG ATGAAGACGT GGACACCACG CTCATCATGC AGAGACTGGA GGCCGAGAAG 
AGAGCACGGC AGACTGCTGT TCCAGCAATC TCTGTACTAG ACTTGCTGCA GGACGAGGAG 
CGACGACGGA AGCAGCAGCT GGAGGAAATC CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA 
CAGGAGGAGG AGAGACGCTG GAGAGAGGAG GAGAGAGCCA GGAGAGAGGC TGACGAGAAG 
AGGAGACAGG AGGAAGAGTA CTACACCCGC CTGGAGGCAG AGCGGCGCCG GCAGCACGAG 
GAAGCTGAGC GCAAGCTGCC GACGCCAGAT GAGACGGGTC TGTACAGACC CCCTTTACCC 
CGGGACTACC AGCCCCCTTC TCCCTCAAGC GCACCCGCCA CTAACCACAC CACCAGCGCC 
CCCCCGCCTC CGCCTCAGAG AAACACCTCC TACCTCAAAA CCCAGGTCGC CTCCCCAGAC 
ACCATTTACA CTGCCAAGTT TGTCTCGTAC AATGACGAGG AGGAGGAGGA GGATGAGGAA 
GATGCGGGCC TAACAGGTCA GGTTAAGCTC TCGGCCACCC GCAAGTCCTA CGGCGACCTT 
CCGCCTGCCC CAAAGCCCCA GCCGCCGCCA ACCGCGCGCA AGCCCCGTCC CGTCTCAGAT 
GGCATCTTTC TGTCCAACTC ATTCCAGCTG CCCGCTGGGC TCAATGCCAA CAGTACTGCT 
CTCAAGACAG GGCAGCCCCC TCTCCCCTTC ACCAAACCCA GCTTCATCCC ATCAGCCAAC 
TCCAAAGGAT TTAATTCATA CACTGGAAAC ACAACAGGAC TAGTATCAGG AGAGGTGTAT 
AAAGACCCGC GAGACAAGTG GACCAAAGGC AGCCCGGATC AAGAGAACAC AATAGGAGGA 
GCTCCAGAAA ACCTGACCTT CAAGGAGCGC CAGAGACTCT TCTCGCAGGG GAAAGAGGTC 
TCCAATAAAG TCAAAGCGTC CCGAAAGCTG ATGGAGCTGG AGAACGAACT CAACATAAAA 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208103.1
CDS	32..5977
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352614.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352614.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGATGGC 
TCCGACTCCA GGGACAAACC CAAACTCTAC AGACTACAGC ACAGCGTTAC TGAGGTCGGA 
TCTGACCGGA GTGAAGATGG AAACATCCAG CTGTTTGGGC CTGGGATTCT CCCTCATCAC 
TGTGACCTTA TGCATGCGGA CGGCTTGGTG ACCATAACCC CAGCCAATAT GGACGCAGAG 
ACATTTGTGG ATGGACAGCG GATCGCTGAC ACTACAGTTC TGCGCAGTGG GGTGACCGTC 
CAGTTCGGAG CAACTCACGT CTTCAAATTC GTAGACCCCA GTTATGACCA GAGCATGTCC 
AAAAGAGACC CCGGGCCCAT GATGAAAGGC AGGCACAAAT CAGGGAGTGT CCCAGAGACG 
ACCTTTGACC TCCACGGGGA CATTCACAGT GGCTCTGCTC TCCCTACAAG CAAGAGCTCA 
GGGAAGCTGG AGATGGAGCG GTCAGGAAAC AGCAGTGAAA GAGGCCTGGT GAAGCCCATG 
ATTCGTGGAG AGCAGCAGGA CTTTAGCAAG CATGATGGAA GGTCTCAGGA CGGCCCGGAG 
CTCACTCTGC CCGCCAGCAT CGAGTTCAGG GAAAACTCCG AGGATGCCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCGACAGTG CACTTCAAGC TTTCTCCCAC ATACGTGCTG 
TACATGACCT GCCGTTATGT CATGTCCAGC CAATACCGGC CCGACATCAG TCCCTCCGAA 
CGCACACACA AAGTCATCGC CATTGTCAAC AAGATGGTGA GCATGATGGA AGGAGTCATC 
CAGGAGATTG CTGAAGGTGA TCAGAAACAG AAGAACATCG CTGGAGCTCT GGCGTTTTGG 
ATGGCGAACG CTTCTGAGCT GCTGAACTTC ATCAAACAGG ACAAGGATCT GAGTCGCATC 
ACGCTGGATG CTCAGGATGT GCTCGCTCAT CTTGTTCAGA TGGCCTTCAA GTACCTGGTG 
CACTGTCTGC AGTCAGATCT CAATAACTAC ATGCCAGCTT TCCTGGAAGA TCCCGACGAG 
CAAAACCCAC AGAGGCCTAA GATAGAGGAT GTTCTGCACA CGCTGACGGG TGCCATGTCT 
CTGCTGCGCC GATGCCGTGT GAACGCGGCG CTCACCATCC AACTGTTTTC GCAGCTCTTC 
CACTTCGTCA ACATGTGGCT CTTTAACAAG CTGGTGACGG AGGCCGATTC TGGCCTGTGC 
TCACACTACT GGGGCGCCAT CCTTCGCCAG CAGCTCAGTC ATATTGAGGC CTGGGCTGAG 
AAACAAGGCT TGGAGCTGGC TGCAGACTGC CACCTCAGCC GCATAGTCCA GGCGACGACC 
CTCCTTACTA TGGACAAGTA CTCCATGCAA GATGTCCAGA ACATCAACAA CACCTGCTTC 
AAGCTCAACT CTCTTCAGCT CAACGCTCTG CTGAGCAACT ACCACTGCGC TCCTGACGAA 
CCCTACATTC CTCCTGAGCT GATTGATCAC GTGGTGGCCG TGGCCGAAAA CACTGCAGAC 
GAACTGGCCC GAAGCGACGG GCGGGAGGTT CAGCTGGAAG AGGACCCCGA CCTTCAGCTG 
CCTTTCTTGC TGCCTGAGGA CGGCTACTCG TGCGATGTGG TGCGCAACAT TCCCAACGGA 
TTCCAAGAGT TTCTAGAGCC ACTTTGCCAG AGAGGTTTCT GTAGATTGAC TCCTCATCCA 
CGCTCGCCGG GAACTTGGAC CATTTATTTT GAAGGAGCCA ACTGTGACAG TCACTTCTCA 
GCCGACACCT CAGAACTGCC AATGCGGAAG GAACCGGAAA TTGTGACCGT GACTTTGAAG 
AAACATAATG GAATGGGCCT CAGTATTGTG GCGGCCAAGG GTGCCGGGCA GGACAAACTT 
GGTATTTACA TCAAATCTGT TGTCAAAGGA GGTGCAGCAG ACATGGATGG GCGTCTGGCT 
GCTGGAGACC AGTTGCTTAG CGTGGATGGA CGTAGTCTTG TCGGCCTCTC GCAGGAGAGA 
GCGGCAGAAT TGATGACACG AACTGGGTCT GTTGTAACGC TTGAGGTGGC CAAACAAGGA 
GCCATTTATC ACGGTCTGGC CACACTTCTT AACCAGCCAT CCCCCATGAT GCCTCGCGCG 
TCAGACCGTG GACGTGGCGA CAAAGGAAAG CTGAGACCAA AGAGTGAGGG CTTTGAGCTC 
TACAACAACT CTGTACAGAA CGGCTCACCT GAGAGCCCAC AGGGCACGTG GGACACCTAT 
CCTGAACCAA AGAAAGAGGA CAGGTTGTTG AAGAACCGAG CAGACCATCG ATCCAGCCCA 
AATGTGGCCA ATCAGGCTCA GAGTCCAGGC GGGAAGCCTG TTTATCCGGG AGCTCCAGGA 
AAGATCACTT CGGTCTCGAC GGGTAACCTC TGCCCTGATG AGGAGCCCTC TCCACCCCGT 
CCAGAGGCCT ATCCCATCCC CACTCAGACT TACCCTAGGG AATACTTCAC CTTCCCAGCC 
TCCAAATCTC AGGACAGGAT GGGCCCTGGC CAGCCCTGGC AAAACAACGA ACAAGAGCCT 
CTTCCCCCTA TGGATAACCA CTCCAACAAC AGCATGGTTA TGCAGCGGGT GGCGCGATCT 
CAGGAGGAGC TGTGTGACAT GCCGGGCGGG TACCCAGGTG AACGTCTGCG CCAGGAAGAT 
CTAATGCTCC TCCAGCAGCA GCAGCAGCGT GAAGCTGAGT ACCAGCGAGG TGTTGGAGAA 
CACTGGAACC ACCAGGTGTC TTCCTCAGTG GAGTCCAGCA CCTCCAGCCA AGAGCACCTC 
AACTTCTCCT CATCCTCTGG CTCCAGGACC CAGGGCAACC ACAAGAGTGG CCCAGGCCGC 
TGGAAGACCC CACTTGCACC TCATTCCGGC CCTGTCTCCC AACCCTCCCG TTCAGACCTT 
CCTCCACCTC CACCTCCTCC ACCAGCACAC TACGATTTTG AGCCGCAAAC CGACCTGCCG 
CTTCCACCGC CACCTTCAGC TACACAACAA CAGCAGCAGG CTGCAGCCGA CCGCAAGAAA 
CGTGAAGACC AACAGCGCTG GTATGAGAAG GAAAAAGCTC GTCTTGAAGA GGAGCGAGAG 
AGGAAGAGAC GAGATCAGGA AAGGAAATTG GTGCAAATCC GAAACCCTTC GGTTTCTGGT 
CCAGTTATGC ACAATAACCA GCATGGCCCG GTGCCACCAT CTCCACAAAT GCCTCCCATG 
ACACTTCCCC AATCATACCT TCCTCCAGTC CAACAACCTC CGCCACCACA ACCGGCTCGA 
CCCGATAAAC TCTCCAGTCT TCCTCGCCAG CCTCCGCAAG ACACCGTAAT CCGGGACCTC 
CTGCCGCAGC AGCAGCCGCG CACCATCGAG AGACGCGACC TGCAGTACAT CACCATCAGC 
AAGGAGGAGT TATCGGCCAG CGACAGCCTG TCACCCGACC CCTGGAAGAG GGACGCCCGG 
GAAAAACTGG AGAAGCAGCA GCAGCTTCAC ATCGTTGACC TGCTGGACAA GGAGATCCAG 
GAGCTGCAGG CTAAACCCGA GCGCACGGCC GAGGACAACG ACCGCCTGCG CAAACTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAACGAGGA GGACGAGGAG 
GAGGAAGACG ATGAAGACGT GGACACCACG CTCATCATGC AGAGACTGGA GGCCGAGAAG 
AGAGCACGGC AGACTGCTGT TCCAGCAATC TCTGTACTAG ACTTGCTGCA GGACGAGGAG 
CGACGACGGA AGCAGCAGCT GGAGGAAATC CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA 
CAGGAGGAGG AGAGACGCTG GAGAGAGGAG GAGAGAGCCA GGAGAGAGGC TGACGAGAAG 
AGGAGACAGG AGGAAGAGTA CTACACCCGC CTGGAGGCAG AGCGGCGCCG GCAGCACGAG 
GAAGCTGAGC GCAAGCTGCC GACGCCAGAT GAGACGGGTC TGTACAGACC CCCTTTACCC 
CGGGACTACC AGCCCCCTTC TCCCTCAAGC GCACCCGCCA CTAACCACAC CACCAGCGCC 
CCCCCGCCTC CGCCTCAGAG AAACACCTCC TACCTCAAAA CCCAGGTCGC CTCCCCAGAC 
ACCATTTACA CTGCCAAGTT TGTCTCGTAC AATGACGAGG AGGAGGAGGA GGATGAGGAA 
GATGCGGGCC TAACAGGTCA GGTTAAGCTC TCGGCCACCC GCAAGTCCTA CGGCGACCTT 
CCGCCTGCCC CAAAGCCCCA GCCGCCGCCA ACCGCGCGCA AGCCCCGTCC CGTCTCAGAT 
GGCATCTTTC TGTCCAACTC ATTCCAGCTG CCCGCTGGGC TCAATGCCAA CAGTACTGCT 
CTCAAGACAG GGCAGCCCCC TCTCCCCTTC ACCAAACCCA GCTTCATCCC ATCAGCCAAC 
TCCAAAGGAT TTAATTCATA CACTGGAAAC ACAACAGGAC TAGTATCAGG AGAGGTGTAT 
AAAGACCCGC GAGACAAGTG GACCAAAGGC AGCCCGGATC AAGAGAACAC AATAGGAGGA 
GCTCCAGAAA ACCTGACCTT CAAGGAGCGC CAGAGACTCT TCTCGCAGGG GAAAGAGGTC 
TCCAATAAAG TCAAAGCGTC CCGAAAGCTG ATGGAGCTGG AGAACGAACT CAACATAAAA 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352615.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208104.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X7, mRNA.

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CloneID	ODa42827
Clone ID Related Accession (Same CDS sequence)	XM_017352615.1
Accession Version	XM_017352615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCAGACTGCT 
GTTCCAGCAA TCTCTGTACT AGACTTGCTG CAGGACGAGG AGCGACGACG GAAGCAGCAG 
CTGGAGGAAA TCCGCAAGCG CGAAGCAGAA GAGCGAGCCA AACAGGAGGA GGAGAGACGC 
TGGAGAGAGG AGGAGAGAGC CAGGAGAGAG GCTGACGAGA AGAGGAGACA GGAGGAAGAG 
TACTACACCC GCCTGGAGGC AGAGCGGCGC CGGCAGCACG AGGAAGCTGA GCGCAAGCTG 
CCGACGCCAG ATGAGACGGG TCTGTACAGA CCCCCTTTAC CCCGGGACTA CCAGCCCCCT 
TCTCCCTCAA GCGCACCCGC CACTAACCAC ACCACCAGCG CCCCCCCGCC TCCGCCTCAG 
AGAAACACCT CCTACCTCAA AACCCAGGTC GCCTCCCCAG ACACCATTTA CACTGCCAAG 
TTTGTCTCGT ACAATGACGA GGAGGAGGAG GAGGATGAGG AAGATGCGGG CCTAACAGGA 
TTTAATTCAT ACACTGGAAA CACAACAGGA CTAGTATCAG GAGAGGTGTA TAAAGACCCG 
CGAGACAAGT GGACCAAAGG CAGCCCGGAT CAAGAGAACA CAATAGGAGG AGCTCCAGAA 
AACCTGACCT TCAAGGAGCG CCAGAGACTC TTCTCGCAGG GGAAAGAGGT CTCCAATAAA 
GTCAAAGCGT CCCGAAAGCT GATGGAGCTG GAGAACGAAC TCAACATAAA ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208104.1
CDS	32..5788
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352615.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352615.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCAGACTGCT 
GTTCCAGCAA TCTCTGTACT AGACTTGCTG CAGGACGAGG AGCGACGACG GAAGCAGCAG 
CTGGAGGAAA TCCGCAAGCG CGAAGCAGAA GAGCGAGCCA AACAGGAGGA GGAGAGACGC 
TGGAGAGAGG AGGAGAGAGC CAGGAGAGAG GCTGACGAGA AGAGGAGACA GGAGGAAGAG 
TACTACACCC GCCTGGAGGC AGAGCGGCGC CGGCAGCACG AGGAAGCTGA GCGCAAGCTG 
CCGACGCCAG ATGAGACGGG TCTGTACAGA CCCCCTTTAC CCCGGGACTA CCAGCCCCCT 
TCTCCCTCAA GCGCACCCGC CACTAACCAC ACCACCAGCG CCCCCCCGCC TCCGCCTCAG 
AGAAACACCT CCTACCTCAA AACCCAGGTC GCCTCCCCAG ACACCATTTA CACTGCCAAG 
TTTGTCTCGT ACAATGACGA GGAGGAGGAG GAGGATGAGG AAGATGCGGG CCTAACAGGA 
TTTAATTCAT ACACTGGAAA CACAACAGGA CTAGTATCAG GAGAGGTGTA TAAAGACCCG 
CGAGACAAGT GGACCAAAGG CAGCCCGGAT CAAGAGAACA CAATAGGAGG AGCTCCAGAA 
AACCTGACCT TCAAGGAGCG CCAGAGACTC TTCTCGCAGG GGAAAGAGGT CTCCAATAAA 
GTCAAAGCGT CCCGAAAGCT GATGGAGCTG GAGAACGAAC TCAACATAAA ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352616.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208105.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X8, mRNA.

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CloneID	ODa42828
Clone ID Related Accession (Same CDS sequence)	XM_017352616.1
Accession Version	XM_017352616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCAGACTGCT 
GTTCCAGCAA TCTCTGTACT AGACTTGCTG CAGGACGAGG AGCGACGACG GAAGCAGCAG 
CTGGAGGAAA TCCGCAAGCG CGAAGCAGAA GAGCGAGCCA AACAGGAGGA GGAGAGACGC 
TGGAGAGAGG AGGAGAGAGC CAGGAGAGAG GCTGACGAGA AGAGGAGACA GGAGGAAGAG 
TACTACACCC GCCTGGAGGC AGAGCGGCGC CGGCAGCACG AGGAAGCTGA GCGCAAGCTG 
CCGACGCCAG ATGAGACGGG TCTGTACAGA CCCCCTTTAC CCCGGGACTA CCAGCCCCCT 
TCTCCCTCAA GCGCACCCGC CACTAACCAC ACCACCAGCG CCCCCCCGCC TCCGCCTCAG 
AGAAACACCT CCTACCTCAA AACCCAGGTC GCCTCCCCAG ACACCATTTA CACTGCCAAG 
TTTGTCTCGT ACAATGACGA GGAGGAGGAG GAGGATGAGG AAGATGCGGG CCTAACAGGA 
TTTAATTCAT ACACTGGAAA CACAACAGGA CTAGTATCAG GAGAGGTGTA TAAAGACCCG 
CGAGACAAGT GGACCAAAGG CCCGGATCAA GAGAACACAA TAGGAGGAGC TCCAGAAAAC 
CTGACCTTCA AGGAGCGCCA GAGACTCTTC TCGCAGGGGA AAGAGGTCTC CAATAAAGTC 
AAAGCGTCCC GAAAGCTGAT GGAGCTGGAG AACGAACTCA ACATAAAACA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208105.1
CDS	35..5788
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352616.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352616.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCAGACTGCT 
GTTCCAGCAA TCTCTGTACT AGACTTGCTG CAGGACGAGG AGCGACGACG GAAGCAGCAG 
CTGGAGGAAA TCCGCAAGCG CGAAGCAGAA GAGCGAGCCA AACAGGAGGA GGAGAGACGC 
TGGAGAGAGG AGGAGAGAGC CAGGAGAGAG GCTGACGAGA AGAGGAGACA GGAGGAAGAG 
TACTACACCC GCCTGGAGGC AGAGCGGCGC CGGCAGCACG AGGAAGCTGA GCGCAAGCTG 
CCGACGCCAG ATGAGACGGG TCTGTACAGA CCCCCTTTAC CCCGGGACTA CCAGCCCCCT 
TCTCCCTCAA GCGCACCCGC CACTAACCAC ACCACCAGCG CCCCCCCGCC TCCGCCTCAG 
AGAAACACCT CCTACCTCAA AACCCAGGTC GCCTCCCCAG ACACCATTTA CACTGCCAAG 
TTTGTCTCGT ACAATGACGA GGAGGAGGAG GAGGATGAGG AAGATGCGGG CCTAACAGGA 
TTTAATTCAT ACACTGGAAA CACAACAGGA CTAGTATCAG GAGAGGTGTA TAAAGACCCG 
CGAGACAAGT GGACCAAAGG CCCGGATCAA GAGAACACAA TAGGAGGAGC TCCAGAAAAC 
CTGACCTTCA AGGAGCGCCA GAGACTCTTC TCGCAGGGGA AAGAGGTCTC CAATAAAGTC 
AAAGCGTCCC GAAAGCTGAT GGAGCTGGAG AACGAACTCA ACATAAAACA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352617.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208106.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X9, mRNA.

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CloneID	ODa42829
Clone ID Related Accession (Same CDS sequence)	XM_017352617.1
Accession Version	XM_017352617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCTGCAGGAC 
GAGGAGCGAC GACGGAAGCA GCAGCTGGAG GAAATCCGCA AGCGCGAAGC AGAAGAGCGA 
GCCAAACAGG AGGAGGAGAG ACGCTGGAGA GAGGAGGAGA GAGCCAGGAG AGAGGCTGAC 
GAGAAGAGGA GACAGGAGGA AGAGTACTAC ACCCGCCTGG AGGCAGAGCG GCGCCGGCAG 
CACGAGGAAG CTGAGCGCAA GCTGCCGACG CCAGATGAGA CGGGTCTGTA CAGACCCCCT 
TTACCCCGGG ACTACCAGCC CCCTTCTCCC TCAAGCGCAC CCGCCACTAA CCACACCACC 
AGCGCCCCCC CGCCTCCGCC TCAGAGAAAC ACCTCCTACC TCAAAACCCA GGTCGCCTCC 
CCAGACACCA TTTACACTGC CAAGTTTGTC TCGTACAATG ACGAGGAGGA GGAGGAGGAT 
GAGGAAGATG CGGGCCTAAC AGGATTTAAT TCATACACTG GAAACACAAC AGGACTAGTA 
TCAGGAGAGG TGTATAAAGA CCCGCGAGAC AAGTGGACCA AAGGCCCGGA TCAAGAGAAC 
ACAATAGGAG GAGCTCCAGA AAACCTGACC TTCAAGGAGC GCCAGAGACT CTTCTCGCAG 
GGGAAAGAGG TCTCCAATAA AGTCAAAGCG TCCCGAAAGC TGATGGAGCT GGAGAACGAA 
CTCAACATAA AACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208106.1
CDS	32..5749
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352617.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352617.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGGAGAT TGCTGAAGGT 
GATCAGAAAC AGAAGAACAT CGCTGGAGCT CTGGCGTTTT GGATGGCGAA CGCTTCTGAG 
CTGCTGAACT TCATCAAACA GGACAAGGAT CTGAGTCGCA TCACGCTGGA TGCTCAGGAT 
GTGCTCGCTC ATCTTGTTCA GATGGCCTTC AAGTACCTGG TGCACTGTCT GCAGTCAGAT 
CTCAATAACT ACATGCCAGC TTTCCTGGAA GATCCCGACG AGCAAAACCC ACAGAGGCCT 
AAGATAGAGG ATGTTCTGCA CACGCTGACG GGTGCCATGT CTCTGCTGCG CCGATGCCGT 
GTGAACGCGG CGCTCACCAT CCAACTGTTT TCGCAGCTCT TCCACTTCGT CAACATGTGG 
CTCTTTAACA AGCTGGTGAC GGAGGCCGAT TCTGGCCTGT GCTCACACTA CTGGGGCGCC 
ATCCTTCGCC AGCAGCTCAG TCATATTGAG GCCTGGGCTG AGAAACAAGG CTTGGAGCTG 
GCTGCAGACT GCCACCTCAG CCGCATAGTC CAGGCGACGA CCCTCCTTAC TATGGACAAG 
TACTCCATGC AAGATGTCCA GAACATCAAC AACACCTGCT TCAAGCTCAA CTCTCTTCAG 
CTCAACGCTC TGCTGAGCAA CTACCACTGC GCTCCTGACG AACCCTACAT TCCTCCTGAG 
CTGATTGATC ACGTGGTGGC CGTGGCCGAA AACACTGCAG ACGAACTGGC CCGAAGCGAC 
GGGCGGGAGG TTCAGCTGGA AGAGGACCCC GACCTTCAGC TGCCTTTCTT GCTGCCTGAG 
GACGGCTACT CGTGCGATGT GGTGCGCAAC ATTCCCAACG GATTCCAAGA GTTTCTAGAG 
CCACTTTGCC AGAGAGGTTT CTGTAGATTG ACTCCTCATC CACGCTCGCC GGGAACTTGG 
ACCATTTATT TTGAAGGAGC CAACTGTGAC AGTCACTTCT CAGCCGACAC CTCAGAACTG 
CCAATGCGGA AGGAACCGGA AATTGTGACC GTGACTTTGA AGAAACATAA TGGAATGGGC 
CTCAGTATTG TGGCGGCCAA GGGTGCCGGG CAGGACAAAC TTGGTATTTA CATCAAATCT 
GTTGTCAAAG GAGGTGCAGC AGACATGGAT GGGCGTCTGG CTGCTGGAGA CCAGTTGCTT 
AGCGTGGATG GACGTAGTCT TGTCGGCCTC TCGCAGGAGA GAGCGGCAGA ATTGATGACA 
CGAACTGGGT CTGTTGTAAC GCTTGAGGTG GCCAAACAAG GAGCCATTTA TCACGGTCTG 
GCCACACTTC TTAACCAGCC ATCCCCCATG ATGCCTCGCG CGTCAGACCG TGGACGTGGC 
GACAAAGGAA AGCTGAGACC AAAGAGTGAG GGCTTTGAGC TCTACAACAA CTCTGTACAG 
AACGGCTCAC CTGAGAGCCC ACAGGGCACG TGGGACACCT ATCCTGAACC AAAGAAAGAG 
GACAGGTTGT TGAAGAACCG AGCAGACCAT CGATCCAGCC CAAATGTGGC CAATCAGGCT 
CAGAGTCCAG GCGGGAAGCC TGTTTATCCG GGAGCTCCAG GAAAGATCAC TTCGGTCTCG 
ACGGGTAACC TCTGCCCTGA TGAGGAGCCC TCTCCACCCC GTCCAGAGGC CTATCCCATC 
CCCACTCAGA CTTACCCTAG GGAATACTTC ACCTTCCCAG CCTCCAAATC TCAGGACAGG 
ATGGGCCCTG GCCAGCCCTG GCAAAACAAC GAACAAGAGC CTCTTCCCCC TATGGATAAC 
CACTCCAACA ACAGCATGGT TATGCAGCGG GTGGCGCGAT CTCAGGAGGA GCTGTGTGAC 
ATGCCGGGCG GGTACCCAGG TGAACGTCTG CGCCAGGAAG ATCTAATGCT CCTCCAGCAG 
CAGCAGCAGC GTGAAGCTGA GTACCAGCGA GGTGTTGGAG AACACTGGAA CCACCAGGTG 
TCTTCCTCAG TGGAGTCCAG CACCTCCAGC CAAGAGCACC TCAACTTCTC CTCATCCTCT 
GGCTCCAGGA CCCAGGGCAA CCACAAGAGT GGCCCAGGCC GCTGGAAGAC CCCACTTGCA 
CCTCATTCCG GCCCTGTCTC CCAACCCTCC CGTTCAGACC TTCCTCCACC TCCACCTCCT 
CCACCAGCAC ACTACGATTT TGAGCCGCAA ACCGACCTGC CGCTTCCACC GCCACCTTCA 
GCTACACAAC AACAGCAGCA GGCTGCAGCC GACCGCAAGA AACGTGAAGA CCAACAGCGC 
TGGTATGAGA AGGAAAAAGC TCGTCTTGAA GAGGAGCGAG AGAGGAAGAG ACGAGATCAG 
GAAAGGAAAT TGGTGCAAAT CCGAAACCCT TCGGTTTCTG GTCCAGTTAT GCACAATAAC 
CAGCATGGCC CGGTGCCACC ATCTCCACAA ATGCCTCCCA TGACACTTCC CCAATCATAC 
CTTCCTCCAG TCCAACAACC TCCGCCACCA CAACCGGCTC GACCCGATAA ACTCTCCAGT 
CTTCCTCGCC AGCCTCCGCA AGACACCGTA ATCCGGGACC TCCTGCCGCA GCAGCAGCCG 
CGCACCATCG AGAGACGCGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GTTATCGGCC 
AGCGACAGCC TGTCACCCGA CCCCTGGAAG AGGGACGCCC GGGAAAAACT GGAGAAGCAG 
CAGCAGCTTC ACATCGTTGA CCTGCTGGAC AAGGAGATCC AGGAGCTGCA GGCTAAACCC 
GAGCGCACGG CCGAGGACAA CGACCGCCTG CGCAAACTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAACGAG GAGGACGAGG AGGAGGAAGA CGATGAAGAC 
GTGGACACCA CGCTCATCAT GCAGAGACTG GAGGCCGAGA AGAGAGCACG GCTGCAGGAC 
GAGGAGCGAC GACGGAAGCA GCAGCTGGAG GAAATCCGCA AGCGCGAAGC AGAAGAGCGA 
GCCAAACAGG AGGAGGAGAG ACGCTGGAGA GAGGAGGAGA GAGCCAGGAG AGAGGCTGAC 
GAGAAGAGGA GACAGGAGGA AGAGTACTAC ACCCGCCTGG AGGCAGAGCG GCGCCGGCAG 
CACGAGGAAG CTGAGCGCAA GCTGCCGACG CCAGATGAGA CGGGTCTGTA CAGACCCCCT 
TTACCCCGGG ACTACCAGCC CCCTTCTCCC TCAAGCGCAC CCGCCACTAA CCACACCACC 
AGCGCCCCCC CGCCTCCGCC TCAGAGAAAC ACCTCCTACC TCAAAACCCA GGTCGCCTCC 
CCAGACACCA TTTACACTGC CAAGTTTGTC TCGTACAATG ACGAGGAGGA GGAGGAGGAT 
GAGGAAGATG CGGGCCTAAC AGGATTTAAT TCATACACTG GAAACACAAC AGGACTAGTA 
TCAGGAGAGG TGTATAAAGA CCCGCGAGAC AAGTGGACCA AAGGCCCGGA TCAAGAGAAC 
ACAATAGGAG GAGCTCCAGA AAACCTGACC TTCAAGGAGC GCCAGAGACT CTTCTCGCAG 
GGGAAAGAGG TCTCCAATAA AGTCAAAGCG TCCCGAAAGC TGATGGAGCT GGAGAACGAA 
CTCAACATAA AACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352618.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208107.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X10, mRNA.

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CloneID	ODa42831
Clone ID Related Accession (Same CDS sequence)	XM_017352618.1
Accession Version	XM_017352618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AGAGGAAACT GGACTTGGAT GAGAAACCAC TGGTCGTGCA GCTGAACTGG 
AATAAAGACG ACAGAGAGGG CCGCTTCGTT CTCAAGAACG AGAATGACAT TATGCCCAAG 
AGAACACTGA CAAACGGTCC TGAAAAGGAG AAAGAGGGGG TGATCCAGAA CTTCAAAAGA 
ACACTGTCCA AGAAGGAGAA GAAGAAAGAG AAGAAGAGGG AGAAGGAGGC TTTTTCCAGA 
ATCCCAGATG GAGACGATCA TGTGTTGAAC CGCGAGGATG GAGAGAACTC CAGATTAGCA 
GCTGAGGTTT ATAAAGACAT GCCGGAGACC AGTTTCACAA GGACCATTTC TAACCCCGAG 
GTGGTGATGA AGAGAAGACG ACAGCAGAAG CTGGAGAAGA GGATGCAGGA GTTTCGGAGT 
GAAGACGGTC GACCCGACTC AGGAGGCACT CTGAGGATCT ACGCTGATAG TCTGAAGCCC 
AACATTCCCT ACAAGACCAT CCTGCTCTCA ACTACTGACA TGGCTGACTT TGCTGTGGCC 
GAGGCTCTGG AGAAATATGG CCTGGAGAAG GAAAACCCTA GAGACTACTG CATCGCCCAG 
GTGATTCATG CAAATGATAA AGCAGTGAAG GAGACCATAT TAGATGACAC AGACTGTCCT 
CTGCAGATCC TCAGAGACTG GACGAGTGAT AAAGAGGCCT TAGTTTTCCA GCTGAAGAAG 
CGTCCTCCAG ATTACGCTCC AAGGAAAGGA CGTAAGCCAG ACAACCGGAT GGGGCCGCAG 
CACGGATCCA TTCCTCCTGA GAAACTGCCT TACCTGGTGG AGCTGAGCCC AGGGCGAGGG 
AGTCACTATG CCTACTACTA CCGCCATCAT GAAGATGGCT CCGACTCCAG GGACAAACCC 
AAACTCTACA GACTACAGCA CAGCGTTACT GAGGTCGGAT CTGACCGGAG TGAAGATGGA 
AACATCCAGC TGTTTGGGCC TGGGATTCTC CCTCATCACT GTGACCTTAT GCATGCGGAC 
GGCTTGGTGA CCATAACCCC AGCCAATATG GACGCAGAGA CATTTGTGGA TGGACAGCGG 
ATCGCTGACA CTACAGTTCT GCGCAGTGGG GTGACCGTCC AGTTCGGAGC AACTCACGTC 
TTCAAATTCG TAGACCCCAG TTATGACCAG AGCATGTCCA AAAGAGACCC CGGGCCCATG 
ATGAAAGGCA GGCACAAATC AGGGAGTGTC CCAGAGACGA CCTTTGACCT CCACGGGGAC 
ATTCACAGTG GCTCTGCTCT CCCTACAAGC AAGAGCTCAG GGAAGCTGGA GATGGAGCGG 
TCAGGAAACA GCAGTGAAAG AGGCCTGGTG AAGCCCATGA TTCGTGGAGA GCAGCAGGAC 
TTTAGCAAGC ATGATGGAAG GTCTCAGGAC GGCCCGGAGC TCACTCTGCC CGCCAGCATC 
GAGTTCAGGG AAAACTCCGA GGATGCCTTC CTGTCGGCCA TCATCAACTA CACCAACAGC 
TCGACAGTGC ACTTCAAGCT TTCTCCCACA TACGTGCTGT ACATGACCTG CCGTTATGTC 
ATGTCCAGCC AATACCGGCC CGACATCAGT CCCTCCGAAC GCACACACAA AGTCATCGCC 
ATTGTCAACA AGATGGTGAG CATGATGGAA GGAGTCATCC AGAAACAGAA GAACATCGCT 
GGAGCTCTGG CGTTTTGGAT GGCGAACGCT TCTGAGCTGC TGAACTTCAT CAAACAGGAC 
AAGGATCTGA GTCGCATCAC GCTGGATGCT CAGGATGTGC TCGCTCATCT TGTTCAGATG 
GCCTTCAAGT ACCTGGTGCA CTGTCTGCAG TCAGATCTCA ATAACTACAT GCCAGCTTTC 
CTGGAAGATC CCGACGAGCA AAACCCACAG AGGCCTAAGA TAGAGGATGT TCTGCACACG 
CTGACGGGTG CCATGTCTCT GCTGCGCCGA TGCCGTGTGA ACGCGGCGCT CACCATCCAA 
CTGTTTTCGC AGCTCTTCCA CTTCGTCAAC ATGTGGCTCT TTAACAAGCT GGTGACGGAG 
GCCGATTCTG GCCTGTGCTC ACACTACTGG GGCGCCATCC TTCGCCAGCA GCTCAGTCAT 
ATTGAGGCCT GGGCTGAGAA ACAAGGCTTG GAGCTGGCTG CAGACTGCCA CCTCAGCCGC 
ATAGTCCAGG CGACGACCCT CCTTACTATG GACAAGTACT CCATGCAAGA TGTCCAGAAC 
ATCAACAACA CCTGCTTCAA GCTCAACTCT CTTCAGCTCA ACGCTCTGCT GAGCAACTAC 
CACTGCGCTC CTGACGAACC CTACATTCCT CCTGAGCTGA TTGATCACGT GGTGGCCGTG 
GCCGAAAACA CTGCAGACGA ACTGGCCCGA AGCGACGGGC GGGAGGTTCA GCTGGAAGAG 
GACCCCGACC TTCAGCTGCC TTTCTTGCTG CCTGAGGACG GCTACTCGTG CGATGTGGTG 
CGCAACATTC CCAACGGATT CCAAGAGTTT CTAGAGCCAC TTTGCCAGAG AGGTTTCTGT 
AGATTGACTC CTCATCCACG CTCGCCGGGA ACTTGGACCA TTTATTTTGA AGGAGCCAAC 
TGTGACAGTC ACTTCTCAGC CGACACCTCA GAACTGCCAA TGCGGAAGGA ACCGGAAATT 
GTGACCGTGA CTTTGAAGAA ACATAATGGA ATGGGCCTCA GTATTGTGGC GGCCAAGGGT 
GCCGGGCAGG ACAAACTTGG TATTTACATC AAATCTGTTG TCAAAGGAGG TGCAGCAGAC 
ATGGATGGGC GTCTGGCTGC TGGAGACCAG TTGCTTAGCG TGGATGGACG TAGTCTTGTC 
GGCCTCTCGC AGGAGAGAGC GGCAGAATTG ATGACACGAA CTGGGTCTGT TGTAACGCTT 
GAGGTGGCCA AACAAGGAGC CATTTATCAC GGTCTGGCCA CACTTCTTAA CCAGCCATCC 
CCCATGATGC CTCGCGCGTC AGACCGTGGA CGTGGCGACA AAGGAAAGCT GAGACCAAAG 
AGTGAGGGCT TTGAGCTCTA CAACAACTCT GTACAGAACG GCTCACCTGA GAGCCCACAG 
GGCACGTGGG ACACCTATCC TGAACCAAAG AAAGAGGACA GGTTGTTGAA GAACCGAGCA 
GACCATCGAT CCAGCCCAAA TGTGGCCAAT CAGGCTCAGA GTCCAGGCGG GAAGCCTGTT 
TATCCGGGAG CTCCAGGAAA GATCACTTCG GTCTCGACGG GTAACCTCTG CCCTGATGAG 
GAGCCCTCTC CACCCCGTCC AGAGGCCTAT CCCATCCCCA CTCAGACTTA CCCTAGGGAA 
TACTTCACCT TCCCAGCCTC CAAATCTCAG GACAGGATGG GCCCTGGCCA GCCCTGGCAA 
AACAACGAAC AAGAGCCTCT TCCCCCTATG GATAACCACT CCAACAACAG CATGGTTATG 
CAGCGGGTGG CGCGATCTCA GGAGGAGCTG TGTGACATGC CGGGCGGGTA CCCAGGTGAA 
CGTCTGCGCC AGGAAGATCT AATGCTCCTC CAGCAGCAGC AGCAGCGTGA AGCTGAGTAC 
CAGCGAGGTG TTGGAGAACA CTGGAACCAC CAGGTGTCTT CCTCAGTGGA GTCCAGCACC 
TCCAGCCAAG AGCACCTCAA CTTCTCCTCA TCCTCTGGCT CCAGGACCCA GGGCAACCAC 
AAGAGTGGCC CAGGCCGCTG GAAGACCCCA CTTGCACCTC ATTCCGGCCC TGTCTCCCAA 
CCCTCCCGTT CAGACCTTCC TCCACCTCCA CCTCCTCCAC CAGCACACTA CGATTTTGAG 
CCGCAAACCG ACCTGCCGCT TCCACCGCCA CCTTCAGCTA CACAACAACA GCAGCAGGCT 
GCAGCCGACC GCAAGAAACG TGAAGACCAA CAGCGCTGGT ATGAGAAGGA AAAAGCTCGT 
CTTGAAGAGG AGCGAGAGAG GAAGAGACGA GATCAGGAAA GGAAATTGGT GCAAATCCGA 
AACCCTTCGG TTTCTGGTCC AGTTATGCAC AATAACCAGC ATGGCCCGGT GCCACCATCT 
CCACAAATGC CTCCCATGAC ACTTCCCCAA TCATACCTTC CTCCAGTCCA ACAACCTCCG 
CCACCACAAC CGGCTCGACC CGATAAACTC TCCAGTCTTC CTCGCCAGCC TCCGCAAGAC 
ACCGTAATCC GGGACCTCCT GCCGCAGCAG CAGCCGCGCA CCATCGAGAG ACGCGACCTG 
CAGTACATCA CCATCAGCAA GGAGGAGTTA TCGGCCAGCG ACAGCCTGTC ACCCGACCCC 
TGGAAGAGGG ACGCCCGGGA AAAACTGGAG AAGCAGCAGC AGCTTCACAT CGTTGACCTG 
CTGGACAAGG AGATCCAGGA GCTGCAGGCT AAACCCGAGC GCACGGCCGA GGACAACGAC 
CGCCTGCGCA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GACTGCAGGA GTCCAAGCAG 
AACGAGGAGG ACGAGGAGGA GGAAGACGAT GAAGACGTGG ACACCACGCT CATCATGCAG 
AGACTGGAGG CCGAGAAGAG AGCACGGCTG CAGGACGAGG AGCGACGACG GAAGCAGCAG 
CTGGAGGAAA TCCGCAAGCG CGAAGCAGAA GAGCGAGCCA AACAGGAGGA GGAGAGACGC 
TGGAGAGAGG AGGAGAGAGC CAGGAGAGAG GCTGACGAGA AGAGGAGACA GGAGGAAGAG 
TACTACACCC GCCTGGAGGC AGAGCGGCGC CGGCAGCACG AGGAAGCTGA GCGCAAGCTG 
CCGACGCCAG ATGAGACGGG TCTGTACAGA CCCCCTTTAC CCCGGGACTA CCAGCCCCCT 
TCTCCCTCAA GCGCACCCGC CACTAACCAC ACCACCAGCG CCCCCCCGCC TCCGCCTCAG 
AGAAACACCT CCTACCTCAA AACCCAGGTC GCCTCCCCAG ACACCATTTA CACTGCCAAG 
TTTGTCTCGT ACAATGACGA GGAGGAGGAG GAGGATGAGG AAGATGCGGG CCTAACAGGA 
TTTAATTCAT ACACTGGAAA CACAACAGGA CTAGTATCAG GAGAGGTGTA TAAAGACCCG 
CGAGACAAGT GGACCAAAGG CCCGGATCAA GAGAACACAA TAGGAGGAGC TCCAGAAAAC 
CTGACCTTCA AGGAGCGCCA GAGACTCTTC TCGCAGGGGA AAGAGGTCTC CAATAAAGTC 
AAAGCGTCCC GAAAGCTGAT GGAGCTGGAG AACGAACTCA ACATAAAACA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208107.1
CDS	32..5725
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352618.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352618.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AGAGGAAACT GGACTTGGAT GAGAAACCAC TGGTCGTGCA GCTGAACTGG 
AATAAAGACG ACAGAGAGGG CCGCTTCGTT CTCAAGAACG AGAATGACAT TATGCCCAAG 
AGAACACTGA CAAACGGTCC TGAAAAGGAG AAAGAGGGGG TGATCCAGAA CTTCAAAAGA 
ACACTGTCCA AGAAGGAGAA GAAGAAAGAG AAGAAGAGGG AGAAGGAGGC TTTTTCCAGA 
ATCCCAGATG GAGACGATCA TGTGTTGAAC CGCGAGGATG GAGAGAACTC CAGATTAGCA 
GCTGAGGTTT ATAAAGACAT GCCGGAGACC AGTTTCACAA GGACCATTTC TAACCCCGAG 
GTGGTGATGA AGAGAAGACG ACAGCAGAAG CTGGAGAAGA GGATGCAGGA GTTTCGGAGT 
GAAGACGGTC GACCCGACTC AGGAGGCACT CTGAGGATCT ACGCTGATAG TCTGAAGCCC 
AACATTCCCT ACAAGACCAT CCTGCTCTCA ACTACTGACA TGGCTGACTT TGCTGTGGCC 
GAGGCTCTGG AGAAATATGG CCTGGAGAAG GAAAACCCTA GAGACTACTG CATCGCCCAG 
GTGATTCATG CAAATGATAA AGCAGTGAAG GAGACCATAT TAGATGACAC AGACTGTCCT 
CTGCAGATCC TCAGAGACTG GACGAGTGAT AAAGAGGCCT TAGTTTTCCA GCTGAAGAAG 
CGTCCTCCAG ATTACGCTCC AAGGAAAGGA CGTAAGCCAG ACAACCGGAT GGGGCCGCAG 
CACGGATCCA TTCCTCCTGA GAAACTGCCT TACCTGGTGG AGCTGAGCCC AGGGCGAGGG 
AGTCACTATG CCTACTACTA CCGCCATCAT GAAGATGGCT CCGACTCCAG GGACAAACCC 
AAACTCTACA GACTACAGCA CAGCGTTACT GAGGTCGGAT CTGACCGGAG TGAAGATGGA 
AACATCCAGC TGTTTGGGCC TGGGATTCTC CCTCATCACT GTGACCTTAT GCATGCGGAC 
GGCTTGGTGA CCATAACCCC AGCCAATATG GACGCAGAGA CATTTGTGGA TGGACAGCGG 
ATCGCTGACA CTACAGTTCT GCGCAGTGGG GTGACCGTCC AGTTCGGAGC AACTCACGTC 
TTCAAATTCG TAGACCCCAG TTATGACCAG AGCATGTCCA AAAGAGACCC CGGGCCCATG 
ATGAAAGGCA GGCACAAATC AGGGAGTGTC CCAGAGACGA CCTTTGACCT CCACGGGGAC 
ATTCACAGTG GCTCTGCTCT CCCTACAAGC AAGAGCTCAG GGAAGCTGGA GATGGAGCGG 
TCAGGAAACA GCAGTGAAAG AGGCCTGGTG AAGCCCATGA TTCGTGGAGA GCAGCAGGAC 
TTTAGCAAGC ATGATGGAAG GTCTCAGGAC GGCCCGGAGC TCACTCTGCC CGCCAGCATC 
GAGTTCAGGG AAAACTCCGA GGATGCCTTC CTGTCGGCCA TCATCAACTA CACCAACAGC 
TCGACAGTGC ACTTCAAGCT TTCTCCCACA TACGTGCTGT ACATGACCTG CCGTTATGTC 
ATGTCCAGCC AATACCGGCC CGACATCAGT CCCTCCGAAC GCACACACAA AGTCATCGCC 
ATTGTCAACA AGATGGTGAG CATGATGGAA GGAGTCATCC AGAAACAGAA GAACATCGCT 
GGAGCTCTGG CGTTTTGGAT GGCGAACGCT TCTGAGCTGC TGAACTTCAT CAAACAGGAC 
AAGGATCTGA GTCGCATCAC GCTGGATGCT CAGGATGTGC TCGCTCATCT TGTTCAGATG 
GCCTTCAAGT ACCTGGTGCA CTGTCTGCAG TCAGATCTCA ATAACTACAT GCCAGCTTTC 
CTGGAAGATC CCGACGAGCA AAACCCACAG AGGCCTAAGA TAGAGGATGT TCTGCACACG 
CTGACGGGTG CCATGTCTCT GCTGCGCCGA TGCCGTGTGA ACGCGGCGCT CACCATCCAA 
CTGTTTTCGC AGCTCTTCCA CTTCGTCAAC ATGTGGCTCT TTAACAAGCT GGTGACGGAG 
GCCGATTCTG GCCTGTGCTC ACACTACTGG GGCGCCATCC TTCGCCAGCA GCTCAGTCAT 
ATTGAGGCCT GGGCTGAGAA ACAAGGCTTG GAGCTGGCTG CAGACTGCCA CCTCAGCCGC 
ATAGTCCAGG CGACGACCCT CCTTACTATG GACAAGTACT CCATGCAAGA TGTCCAGAAC 
ATCAACAACA CCTGCTTCAA GCTCAACTCT CTTCAGCTCA ACGCTCTGCT GAGCAACTAC 
CACTGCGCTC CTGACGAACC CTACATTCCT CCTGAGCTGA TTGATCACGT GGTGGCCGTG 
GCCGAAAACA CTGCAGACGA ACTGGCCCGA AGCGACGGGC GGGAGGTTCA GCTGGAAGAG 
GACCCCGACC TTCAGCTGCC TTTCTTGCTG CCTGAGGACG GCTACTCGTG CGATGTGGTG 
CGCAACATTC CCAACGGATT CCAAGAGTTT CTAGAGCCAC TTTGCCAGAG AGGTTTCTGT 
AGATTGACTC CTCATCCACG CTCGCCGGGA ACTTGGACCA TTTATTTTGA AGGAGCCAAC 
TGTGACAGTC ACTTCTCAGC CGACACCTCA GAACTGCCAA TGCGGAAGGA ACCGGAAATT 
GTGACCGTGA CTTTGAAGAA ACATAATGGA ATGGGCCTCA GTATTGTGGC GGCCAAGGGT 
GCCGGGCAGG ACAAACTTGG TATTTACATC AAATCTGTTG TCAAAGGAGG TGCAGCAGAC 
ATGGATGGGC GTCTGGCTGC TGGAGACCAG TTGCTTAGCG TGGATGGACG TAGTCTTGTC 
GGCCTCTCGC AGGAGAGAGC GGCAGAATTG ATGACACGAA CTGGGTCTGT TGTAACGCTT 
GAGGTGGCCA AACAAGGAGC CATTTATCAC GGTCTGGCCA CACTTCTTAA CCAGCCATCC 
CCCATGATGC CTCGCGCGTC AGACCGTGGA CGTGGCGACA AAGGAAAGCT GAGACCAAAG 
AGTGAGGGCT TTGAGCTCTA CAACAACTCT GTACAGAACG GCTCACCTGA GAGCCCACAG 
GGCACGTGGG ACACCTATCC TGAACCAAAG AAAGAGGACA GGTTGTTGAA GAACCGAGCA 
GACCATCGAT CCAGCCCAAA TGTGGCCAAT CAGGCTCAGA GTCCAGGCGG GAAGCCTGTT 
TATCCGGGAG CTCCAGGAAA GATCACTTCG GTCTCGACGG GTAACCTCTG CCCTGATGAG 
GAGCCCTCTC CACCCCGTCC AGAGGCCTAT CCCATCCCCA CTCAGACTTA CCCTAGGGAA 
TACTTCACCT TCCCAGCCTC CAAATCTCAG GACAGGATGG GCCCTGGCCA GCCCTGGCAA 
AACAACGAAC AAGAGCCTCT TCCCCCTATG GATAACCACT CCAACAACAG CATGGTTATG 
CAGCGGGTGG CGCGATCTCA GGAGGAGCTG TGTGACATGC CGGGCGGGTA CCCAGGTGAA 
CGTCTGCGCC AGGAAGATCT AATGCTCCTC CAGCAGCAGC AGCAGCGTGA AGCTGAGTAC 
CAGCGAGGTG TTGGAGAACA CTGGAACCAC CAGGTGTCTT CCTCAGTGGA GTCCAGCACC 
TCCAGCCAAG AGCACCTCAA CTTCTCCTCA TCCTCTGGCT CCAGGACCCA GGGCAACCAC 
AAGAGTGGCC CAGGCCGCTG GAAGACCCCA CTTGCACCTC ATTCCGGCCC TGTCTCCCAA 
CCCTCCCGTT CAGACCTTCC TCCACCTCCA CCTCCTCCAC CAGCACACTA CGATTTTGAG 
CCGCAAACCG ACCTGCCGCT TCCACCGCCA CCTTCAGCTA CACAACAACA GCAGCAGGCT 
GCAGCCGACC GCAAGAAACG TGAAGACCAA CAGCGCTGGT ATGAGAAGGA AAAAGCTCGT 
CTTGAAGAGG AGCGAGAGAG GAAGAGACGA GATCAGGAAA GGAAATTGGT GCAAATCCGA 
AACCCTTCGG TTTCTGGTCC AGTTATGCAC AATAACCAGC ATGGCCCGGT GCCACCATCT 
CCACAAATGC CTCCCATGAC ACTTCCCCAA TCATACCTTC CTCCAGTCCA ACAACCTCCG 
CCACCACAAC CGGCTCGACC CGATAAACTC TCCAGTCTTC CTCGCCAGCC TCCGCAAGAC 
ACCGTAATCC GGGACCTCCT GCCGCAGCAG CAGCCGCGCA CCATCGAGAG ACGCGACCTG 
CAGTACATCA CCATCAGCAA GGAGGAGTTA TCGGCCAGCG ACAGCCTGTC ACCCGACCCC 
TGGAAGAGGG ACGCCCGGGA AAAACTGGAG AAGCAGCAGC AGCTTCACAT CGTTGACCTG 
CTGGACAAGG AGATCCAGGA GCTGCAGGCT AAACCCGAGC GCACGGCCGA GGACAACGAC 
CGCCTGCGCA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GACTGCAGGA GTCCAAGCAG 
AACGAGGAGG ACGAGGAGGA GGAAGACGAT GAAGACGTGG ACACCACGCT CATCATGCAG 
AGACTGGAGG CCGAGAAGAG AGCACGGCTG CAGGACGAGG AGCGACGACG GAAGCAGCAG 
CTGGAGGAAA TCCGCAAGCG CGAAGCAGAA GAGCGAGCCA AACAGGAGGA GGAGAGACGC 
TGGAGAGAGG AGGAGAGAGC CAGGAGAGAG GCTGACGAGA AGAGGAGACA GGAGGAAGAG 
TACTACACCC GCCTGGAGGC AGAGCGGCGC CGGCAGCACG AGGAAGCTGA GCGCAAGCTG 
CCGACGCCAG ATGAGACGGG TCTGTACAGA CCCCCTTTAC CCCGGGACTA CCAGCCCCCT 
TCTCCCTCAA GCGCACCCGC CACTAACCAC ACCACCAGCG CCCCCCCGCC TCCGCCTCAG 
AGAAACACCT CCTACCTCAA AACCCAGGTC GCCTCCCCAG ACACCATTTA CACTGCCAAG 
TTTGTCTCGT ACAATGACGA GGAGGAGGAG GAGGATGAGG AAGATGCGGG CCTAACAGGA 
TTTAATTCAT ACACTGGAAA CACAACAGGA CTAGTATCAG GAGAGGTGTA TAAAGACCCG 
CGAGACAAGT GGACCAAAGG CCCGGATCAA GAGAACACAA TAGGAGGAGC TCCAGAAAAC 
CTGACCTTCA AGGAGCGCCA GAGACTCTTC TCGCAGGGGA AAGAGGTCTC CAATAAAGTC 
AAAGCGTCCC GAAAGCTGAT GGAGCTGGAG AACGAACTCA ACATAAAACA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352619.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208108.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X11, mRNA.

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CloneID	ODa42832
Clone ID Related Accession (Same CDS sequence)	XM_017352619.1
Accession Version	XM_017352619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AGAGGAAACT GGACTTGGAT GAGAAACCAC TGGTCGTGCA GCTGAACTGG 
AATAAAGACG ACAGAGAGGG CCGCTTCGTT CTCAAGAACG AGAATGACAT TATGCCCAAG 
AGAACACTGA CAAACGGTCC TGAAAAGGAG AAAGAGGGGG TGATCCAGAA CTTCAAAAGA 
ACACTGTCCA AGAAGGAGAA GAAGAAAGAG AAGAAGAGGG AGAAGGAGGC TTTTTCCAGA 
ATCCCAGATG GAGACGATCA TGTGTTGAAC CGCGAGGATG GAGAGAACTC CAGATTAGCA 
GCTGAGGTTT ATAAAGACAT GCCGGAGACC AGTTTCACAA GGACCATTTC TAACCCCGAG 
GTGGTGATGA AGAGAAGACG ACAGCAGAAG CTGGAGAAGA GGATGCAGGA GTTTCGGAGT 
GAAGACGGTC GACCCGACTC AGGAGGCACT CTGAGGATCT ACGCTGATAG TCTGAAGCCC 
AACATTCCCT ACAAGACCAT CCTGCTCTCA ACTACTGACA TGGCTGACTT TGCTGTGGCC 
GAGGCTCTGG AGAAATATGG CCTGGAGAAG GAAAACCCTA GAGACTACTG CATCGCCCAG 
GTGATTCATG CAAATGATAA AGCAGTGAAG GAGACCATAT TAGATGACAC AGACTGTCCT 
CTGCAGATCC TCAGAGACTG GACGAGTGAT AAAGAGGCCT TAGTTTTCCA GCTGAAGAAG 
CGTCCTCCAG ATTACGCTCC AAGGAAAGGA CGTAAGCCAG ACAACCGGAT GGGGCCGCAG 
CACGGATCCA TTCCTCCTGA GAAACTGCCT TACCTGGTGG AGCTGAGCCC AGGGCGAGGG 
AGTCACTATG CCTACTACTA CCGCCATCAT GAAGATGGCT CCGACTCCAG GGACAAACCC 
AAACTCTACA GACTACAGCA CAGCGTTACT GAGGTCGGAT CTGACCGGAG TGAAGATGGA 
AACATCCAGC TGTTTGGGCC TGGGATTCTC CCTCATCACT GTGACCTTAT GCATGCGGAC 
GGCTTGGTGA CCATAACCCC AGCCAATATG GACGCAGAGA CATTTGTGGA TGGACAGCGG 
ATCGCTGACA CTACAGTTCT GCGCAGTGGG GTGACCGTCC AGTTCGGAGC AACTCACGTC 
TTCAAATTCG TAGACCCCAG TTATGACCAG AGCATGTCCA AAAGAGACCC CGGGCCCATG 
ATGAAAGGCA GGCACAAATC AGGGAGTGTC CCAGAGACGA CCTTTGACCT CCACGGGGAC 
ATTCACAGTG GCTCTGCTCT CCCTACAAGC AAGAGCTCAG GGAAGCTGGA GATGGAGCGG 
TCAGGAAACA GCAGTGAAAG AGGCCTGGTG AAGCCCATGA TTCGTGGAGA GCAGCAGGAC 
TTTAGCAAGC ATGATGGAAG GTCTCAGGAC GGCCCGGAGC TCACTCTGCC CGCCAGCATC 
GAGTTCAGGG AAAACTCCGA GGATGCCTTC CTGTCGGCCA TCATCAACTA CACCAACAGC 
TCGACAGTGC ACTTCAAGCT TTCTCCCACA TACGTGCTGT ACATGACCTG CCGTTATGTC 
ATGTCCAGCC AATACCGGCC CGACATCAGT CCCTCCGAAC GCACACACAA AGTCATCGCC 
ATTGTCAACA AGATGGTGAG CATGATGGAA GGAGTCATCC AGGAGATTGC TGAAGGTGAT 
CAGAAACAGA AGAACATCGC TGGAGCTCTG GCGTTTTGGA TGGCGAACGC TTCTGAGCTG 
CTGAACTTCA TCAAACAGGA CAAGGATCTG AGTCGCATCA CGCTGGATGC TCAGGATGTG 
CTCGCTCATC TTGTTCAGAT GGCCTTCAAG TACCTGGTGC ACTGTCTGCA GTCAGATCTC 
AATAACTACA TGCCAGCTTT CCTGGAAGAT CCCGACGAGC AAAACCCACA GAGGCCTAAG 
ATAGAGGATG TTCTGCACAC GCTGACGGGT GCCATGTCTC TGCTGCGCCG ATGCCGTGTG 
AACGCGGCGC TCACCATCCA ACTGTTTTCG CAGCTCTTCC ACTTCGTCAA CATGTGGCTC 
TTTAACAAGC TGGTGACGGA GGCCGATTCT GGCCTGTGCT CACACTACTG GGGCGCCATC 
CTTCGCCAGC AGCTCAGTCA TATTGAGGCC TGGGCTGAGA AACAAGGCTT GGAGCTGGCT 
GCAGACTGCC ACCTCAGCCG CATAGTCCAG GCGACGACCC TCCTTACTAT GGACAAGTAC 
TCCATGCAAG ATGTCCAGAA CATCAACAAC ACCTGCTTCA AGCTCAACTC TCTTCAGCTC 
AACGCTCTGC TGAGCAACTA CCACTGCGCT CCTGACGAAC CCTACATTCC TCCTGAGCTG 
ATTGATCACG TGGTGGCCGT GGCCGAAAAC ACTGCAGACG AACTGGCCCG AAGCGACGGG 
CGGGAGGTTC AGCTGGAAGA GGACCCCGAC CTTCAGCTGC CTTTCTTGCT GCCTGAGGAC 
GGCTACTCGT GCGATGTGGT GCGCAACATT CCCAACGGAT TCCAAGAGTT TCTAGAGCCA 
CTTTGCCAGA GAGGTTTCTG TAGATTGACT CCTCATCCAC GCTCGCCGGG AACTTGGACC 
ATTTATTTTG AAGGAGCCAA CTGTGACAGT CACTTCTCAG CCGACACCTC AGAACTGCCA 
ATGCGGAAGG AACCGGAAAT TGTGACCGTG ACTTTGAAGA AACATAATGG AATGGGCCTC 
AGTATTGTGG CGGCCAAGGG TGCCGGGCAG GACAAACTTG GTATTTACAT CAAATCTGTT 
GTCAAAGGAG GTGCAGCAGA CATGGATGGG CGTCTGGCTG CTGGAGACCA GTTGCTTAGC 
GTGGATGGAC GTAGTCTTGT CGGCCTCTCG CAGGAGAGAG CGGCAGAATT GATGACACGA 
ACTGGGTCTG TTGTAACGCT TGAGGTGGCC AAACAAGGAG CCATTTATCA CGGTCTGGCC 
ACACTTCTTA ACCAGCCATC CCCCATGATG CCTCGCGCGT CAGACCGTGG ACGTGGCGAC 
AAAGGAAAGC TGAGACCAAA GAGTGAGGGC TTTGAGCTCT ACAACAACTC TGTACAGAAC 
GGCTCACCTG AGAGCCCACA GGGCACGTGG GACACCTATC CTGAACCAAA GAAAGAGGAC 
AGGTTGTTGA AGAACCGAGC AGACCATCGA TCCAGCCCAA ATGTGGCCAA TCAGGCTCAG 
AGTCCAGGCG GGAAGCCTGT TTATCCGGGA GCTCCAGGAA AGATCACTTC GGTCTCGACG 
GGTAACCTCT GCCCTGATGA GGAGCCCTCT CCACCCCGTC CAGAGGCCTA TCCCATCCCC 
ACTCAGACTT ACCCTAGGGA ATACTTCACC TTCCCAGCCT CCAAATCTCA GGACAGGATG 
GGCCCTGGCC AGCCCTGGCA AAACAACGAA CAAGAGCCTC TTCCCCCTAT GGATAACCAC 
TCCAACAACA GCATGGTTAT GCAGCGGGTG GCGCGATCTC AGGAGGAGCT GTGTGACATG 
CCGGGCGGGT ACCCAGGTGA ACGTCTGCGC CAGGAAGATC TAATGCTCCT CCAGCAGCAG 
CAGCAGCGTG AAGCTGAGTA CCAGCGAGGT GTTGGAGAAC ACTGGAACCA CCAGGTGTCT 
TCCTCAGTGG AGTCCAGCAC CTCCAGCCAA GAGCACCTCA ACTTCTCCTC ATCCTCTGGC 
TCCAGGACCC AGGGCAACCA CAAGAGTGGC CCAGGCCGCT GGAAGACCCC ACTTGCACCT 
CATTCCGGCC CTGTCTCCCA ACCCTCCCGT TCAGACCTTC CTCCACCTCC ACCTCCTCCA 
CCAGCACACT ACGATTTTGA GCCGCAAACC GACCTGCCGC TTCCACCGCC ACCTTCAGCT 
ACACAACAAC AGCAGCAGGC TGCAGCCGAC CGCAAGAAAC GTGAAGACCA ACAGCGCTGG 
TATGAGAAGG AAAAAGCTCG TCTTGAAGAG GAGCGAGAGA GGAAGAGACG AGATCAGGAA 
AGGAAATTGG TGCAAATCCG AAACCCTTCG GTTTCTGGTC CAGTTATGCA CAATAACCAG 
CATGGCCCGG TGCCACCATC TCCACAAATG CCTCCCATGA CACTTCCCCA ATCATACCTT 
CCTCCAGTCC AACAACCTCC GCCACCACAA CCGGCTCGAC CCGATAAACT CTCCAGTCTT 
CCTCGCCAGC CTCCGCAAGA CACCGTAATC CGGGACCTCC TGCCGCAGCA GCAGCCGCGC 
ACCATCGAGA GACGCGACCT GCAGTACATC ACCATCAGCA AGGAGGAGTT ATCGGCCAGC 
GACAGCCTGT CACCCGACCC CTGGAAGAGG GACGCCCGGG AAAAACTGGA GAAGCAGCAG 
CAGCTTCACA TCGTTGACCT GCTGGACAAG GAGATCCAGG AGCTGCAGGC TAAACCCGAG 
CGCACGGCCG AGGACAACGA CCGCCTGCGC AAACTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTGCAGG AGTCCAAGCA GAACGAGGAG GACGAGGAGG AGGAAGACGA TGAAGACGTG 
GACACCACGC TCATCATGCA GAGACTGGAG GCCGAGAAGA GAGCACGGCT GCAGGACGAG 
GAGCGACGAC GGAAGCAGCA GCTGGAGGAA ATCCGCAAGC GCGAAGCAGA AGAGCGAGCC 
AAACAGGAGG AGGAGAGACG CTGGAGAGAG GAGGAGAGAG CCAGGAGAGA GGCTGACGAG 
AAGAGGAGAC AGGAGGAAGA GTACTACACC CGCCTGGAGG CAGAGCGGCG CCGGCAGCAC 
GAGGAAGCTG AGCGCAAGCT GCCGACGCCA GATGAGACGG GTCTGTACAG ACCCCCTTTA 
CCCCGGGACT ACCAGCCCCC TTCTCCCTCA AGCGCACCCG CCACTAACCA CACCACCAGC 
GCCCCCCCGC CTCCGCCTCA GAGAAACACC TCCTACCTCA AAACCCAGGT CGCCTCCCCA 
GACACCATTT ACACTGCCAA GTTTGTCTCG TACAATGACG AGGAGGAGGA GGAGGATGAG 
GAAGATGCGG GCCTAACAGG TCAGGTTAAG CTCTCGGCCA CCCGCAAGTC CTACGGCGAC 
CTTCCGCCTG CCCCAAAGCC CCAGCCGCCG CCAACCGCGC GCAAGCCCCG TCCCGTCTCA 
GATGGCATCT TTCTGTCCAA CTCATTCCAG CTGCCCGCTG GGCTCAATGC CAACAGTACT 
GCTCTCAAGA CAGGGCAGCC CCCTCTCCCC TTCACCAAAC CCAGCTTCAT CCCATCAGCC 
AACTCCAAAG GATTTAATTC ATACACTGGA AACACAACAG GACTAGTATC AGGAGAGGTG 
TATAAAGACC CGCGAGACAA GTGGACCAAA GGCAGCCCGG ATCAAGAGAA CACAATAGGA 
GGAGCTCCAG AAAACCTGAC CTTCAAGGAG CGCCAGAGAC TCTTCTCGCA GGGGAAAGAG 
GTCTCCAATA AAGTCAAAGC GTCCCGAAAG CTGATGGAGC TGGAGAACGA ACTCAACATA 
AAACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208108.1
CDS	31..5979
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQEIAEGDQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGSPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352619.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352619.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AGAGGAAACT GGACTTGGAT GAGAAACCAC TGGTCGTGCA GCTGAACTGG 
AATAAAGACG ACAGAGAGGG CCGCTTCGTT CTCAAGAACG AGAATGACAT TATGCCCAAG 
AGAACACTGA CAAACGGTCC TGAAAAGGAG AAAGAGGGGG TGATCCAGAA CTTCAAAAGA 
ACACTGTCCA AGAAGGAGAA GAAGAAAGAG AAGAAGAGGG AGAAGGAGGC TTTTTCCAGA 
ATCCCAGATG GAGACGATCA TGTGTTGAAC CGCGAGGATG GAGAGAACTC CAGATTAGCA 
GCTGAGGTTT ATAAAGACAT GCCGGAGACC AGTTTCACAA GGACCATTTC TAACCCCGAG 
GTGGTGATGA AGAGAAGACG ACAGCAGAAG CTGGAGAAGA GGATGCAGGA GTTTCGGAGT 
GAAGACGGTC GACCCGACTC AGGAGGCACT CTGAGGATCT ACGCTGATAG TCTGAAGCCC 
AACATTCCCT ACAAGACCAT CCTGCTCTCA ACTACTGACA TGGCTGACTT TGCTGTGGCC 
GAGGCTCTGG AGAAATATGG CCTGGAGAAG GAAAACCCTA GAGACTACTG CATCGCCCAG 
GTGATTCATG CAAATGATAA AGCAGTGAAG GAGACCATAT TAGATGACAC AGACTGTCCT 
CTGCAGATCC TCAGAGACTG GACGAGTGAT AAAGAGGCCT TAGTTTTCCA GCTGAAGAAG 
CGTCCTCCAG ATTACGCTCC AAGGAAAGGA CGTAAGCCAG ACAACCGGAT GGGGCCGCAG 
CACGGATCCA TTCCTCCTGA GAAACTGCCT TACCTGGTGG AGCTGAGCCC AGGGCGAGGG 
AGTCACTATG CCTACTACTA CCGCCATCAT GAAGATGGCT CCGACTCCAG GGACAAACCC 
AAACTCTACA GACTACAGCA CAGCGTTACT GAGGTCGGAT CTGACCGGAG TGAAGATGGA 
AACATCCAGC TGTTTGGGCC TGGGATTCTC CCTCATCACT GTGACCTTAT GCATGCGGAC 
GGCTTGGTGA CCATAACCCC AGCCAATATG GACGCAGAGA CATTTGTGGA TGGACAGCGG 
ATCGCTGACA CTACAGTTCT GCGCAGTGGG GTGACCGTCC AGTTCGGAGC AACTCACGTC 
TTCAAATTCG TAGACCCCAG TTATGACCAG AGCATGTCCA AAAGAGACCC CGGGCCCATG 
ATGAAAGGCA GGCACAAATC AGGGAGTGTC CCAGAGACGA CCTTTGACCT CCACGGGGAC 
ATTCACAGTG GCTCTGCTCT CCCTACAAGC AAGAGCTCAG GGAAGCTGGA GATGGAGCGG 
TCAGGAAACA GCAGTGAAAG AGGCCTGGTG AAGCCCATGA TTCGTGGAGA GCAGCAGGAC 
TTTAGCAAGC ATGATGGAAG GTCTCAGGAC GGCCCGGAGC TCACTCTGCC CGCCAGCATC 
GAGTTCAGGG AAAACTCCGA GGATGCCTTC CTGTCGGCCA TCATCAACTA CACCAACAGC 
TCGACAGTGC ACTTCAAGCT TTCTCCCACA TACGTGCTGT ACATGACCTG CCGTTATGTC 
ATGTCCAGCC AATACCGGCC CGACATCAGT CCCTCCGAAC GCACACACAA AGTCATCGCC 
ATTGTCAACA AGATGGTGAG CATGATGGAA GGAGTCATCC AGGAGATTGC TGAAGGTGAT 
CAGAAACAGA AGAACATCGC TGGAGCTCTG GCGTTTTGGA TGGCGAACGC TTCTGAGCTG 
CTGAACTTCA TCAAACAGGA CAAGGATCTG AGTCGCATCA CGCTGGATGC TCAGGATGTG 
CTCGCTCATC TTGTTCAGAT GGCCTTCAAG TACCTGGTGC ACTGTCTGCA GTCAGATCTC 
AATAACTACA TGCCAGCTTT CCTGGAAGAT CCCGACGAGC AAAACCCACA GAGGCCTAAG 
ATAGAGGATG TTCTGCACAC GCTGACGGGT GCCATGTCTC TGCTGCGCCG ATGCCGTGTG 
AACGCGGCGC TCACCATCCA ACTGTTTTCG CAGCTCTTCC ACTTCGTCAA CATGTGGCTC 
TTTAACAAGC TGGTGACGGA GGCCGATTCT GGCCTGTGCT CACACTACTG GGGCGCCATC 
CTTCGCCAGC AGCTCAGTCA TATTGAGGCC TGGGCTGAGA AACAAGGCTT GGAGCTGGCT 
GCAGACTGCC ACCTCAGCCG CATAGTCCAG GCGACGACCC TCCTTACTAT GGACAAGTAC 
TCCATGCAAG ATGTCCAGAA CATCAACAAC ACCTGCTTCA AGCTCAACTC TCTTCAGCTC 
AACGCTCTGC TGAGCAACTA CCACTGCGCT CCTGACGAAC CCTACATTCC TCCTGAGCTG 
ATTGATCACG TGGTGGCCGT GGCCGAAAAC ACTGCAGACG AACTGGCCCG AAGCGACGGG 
CGGGAGGTTC AGCTGGAAGA GGACCCCGAC CTTCAGCTGC CTTTCTTGCT GCCTGAGGAC 
GGCTACTCGT GCGATGTGGT GCGCAACATT CCCAACGGAT TCCAAGAGTT TCTAGAGCCA 
CTTTGCCAGA GAGGTTTCTG TAGATTGACT CCTCATCCAC GCTCGCCGGG AACTTGGACC 
ATTTATTTTG AAGGAGCCAA CTGTGACAGT CACTTCTCAG CCGACACCTC AGAACTGCCA 
ATGCGGAAGG AACCGGAAAT TGTGACCGTG ACTTTGAAGA AACATAATGG AATGGGCCTC 
AGTATTGTGG CGGCCAAGGG TGCCGGGCAG GACAAACTTG GTATTTACAT CAAATCTGTT 
GTCAAAGGAG GTGCAGCAGA CATGGATGGG CGTCTGGCTG CTGGAGACCA GTTGCTTAGC 
GTGGATGGAC GTAGTCTTGT CGGCCTCTCG CAGGAGAGAG CGGCAGAATT GATGACACGA 
ACTGGGTCTG TTGTAACGCT TGAGGTGGCC AAACAAGGAG CCATTTATCA CGGTCTGGCC 
ACACTTCTTA ACCAGCCATC CCCCATGATG CCTCGCGCGT CAGACCGTGG ACGTGGCGAC 
AAAGGAAAGC TGAGACCAAA GAGTGAGGGC TTTGAGCTCT ACAACAACTC TGTACAGAAC 
GGCTCACCTG AGAGCCCACA GGGCACGTGG GACACCTATC CTGAACCAAA GAAAGAGGAC 
AGGTTGTTGA AGAACCGAGC AGACCATCGA TCCAGCCCAA ATGTGGCCAA TCAGGCTCAG 
AGTCCAGGCG GGAAGCCTGT TTATCCGGGA GCTCCAGGAA AGATCACTTC GGTCTCGACG 
GGTAACCTCT GCCCTGATGA GGAGCCCTCT CCACCCCGTC CAGAGGCCTA TCCCATCCCC 
ACTCAGACTT ACCCTAGGGA ATACTTCACC TTCCCAGCCT CCAAATCTCA GGACAGGATG 
GGCCCTGGCC AGCCCTGGCA AAACAACGAA CAAGAGCCTC TTCCCCCTAT GGATAACCAC 
TCCAACAACA GCATGGTTAT GCAGCGGGTG GCGCGATCTC AGGAGGAGCT GTGTGACATG 
CCGGGCGGGT ACCCAGGTGA ACGTCTGCGC CAGGAAGATC TAATGCTCCT CCAGCAGCAG 
CAGCAGCGTG AAGCTGAGTA CCAGCGAGGT GTTGGAGAAC ACTGGAACCA CCAGGTGTCT 
TCCTCAGTGG AGTCCAGCAC CTCCAGCCAA GAGCACCTCA ACTTCTCCTC ATCCTCTGGC 
TCCAGGACCC AGGGCAACCA CAAGAGTGGC CCAGGCCGCT GGAAGACCCC ACTTGCACCT 
CATTCCGGCC CTGTCTCCCA ACCCTCCCGT TCAGACCTTC CTCCACCTCC ACCTCCTCCA 
CCAGCACACT ACGATTTTGA GCCGCAAACC GACCTGCCGC TTCCACCGCC ACCTTCAGCT 
ACACAACAAC AGCAGCAGGC TGCAGCCGAC CGCAAGAAAC GTGAAGACCA ACAGCGCTGG 
TATGAGAAGG AAAAAGCTCG TCTTGAAGAG GAGCGAGAGA GGAAGAGACG AGATCAGGAA 
AGGAAATTGG TGCAAATCCG AAACCCTTCG GTTTCTGGTC CAGTTATGCA CAATAACCAG 
CATGGCCCGG TGCCACCATC TCCACAAATG CCTCCCATGA CACTTCCCCA ATCATACCTT 
CCTCCAGTCC AACAACCTCC GCCACCACAA CCGGCTCGAC CCGATAAACT CTCCAGTCTT 
CCTCGCCAGC CTCCGCAAGA CACCGTAATC CGGGACCTCC TGCCGCAGCA GCAGCCGCGC 
ACCATCGAGA GACGCGACCT GCAGTACATC ACCATCAGCA AGGAGGAGTT ATCGGCCAGC 
GACAGCCTGT CACCCGACCC CTGGAAGAGG GACGCCCGGG AAAAACTGGA GAAGCAGCAG 
CAGCTTCACA TCGTTGACCT GCTGGACAAG GAGATCCAGG AGCTGCAGGC TAAACCCGAG 
CGCACGGCCG AGGACAACGA CCGCCTGCGC AAACTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTGCAGG AGTCCAAGCA GAACGAGGAG GACGAGGAGG AGGAAGACGA TGAAGACGTG 
GACACCACGC TCATCATGCA GAGACTGGAG GCCGAGAAGA GAGCACGGCT GCAGGACGAG 
GAGCGACGAC GGAAGCAGCA GCTGGAGGAA ATCCGCAAGC GCGAAGCAGA AGAGCGAGCC 
AAACAGGAGG AGGAGAGACG CTGGAGAGAG GAGGAGAGAG CCAGGAGAGA GGCTGACGAG 
AAGAGGAGAC AGGAGGAAGA GTACTACACC CGCCTGGAGG CAGAGCGGCG CCGGCAGCAC 
GAGGAAGCTG AGCGCAAGCT GCCGACGCCA GATGAGACGG GTCTGTACAG ACCCCCTTTA 
CCCCGGGACT ACCAGCCCCC TTCTCCCTCA AGCGCACCCG CCACTAACCA CACCACCAGC 
GCCCCCCCGC CTCCGCCTCA GAGAAACACC TCCTACCTCA AAACCCAGGT CGCCTCCCCA 
GACACCATTT ACACTGCCAA GTTTGTCTCG TACAATGACG AGGAGGAGGA GGAGGATGAG 
GAAGATGCGG GCCTAACAGG TCAGGTTAAG CTCTCGGCCA CCCGCAAGTC CTACGGCGAC 
CTTCCGCCTG CCCCAAAGCC CCAGCCGCCG CCAACCGCGC GCAAGCCCCG TCCCGTCTCA 
GATGGCATCT TTCTGTCCAA CTCATTCCAG CTGCCCGCTG GGCTCAATGC CAACAGTACT 
GCTCTCAAGA CAGGGCAGCC CCCTCTCCCC TTCACCAAAC CCAGCTTCAT CCCATCAGCC 
AACTCCAAAG GATTTAATTC ATACACTGGA AACACAACAG GACTAGTATC AGGAGAGGTG 
TATAAAGACC CGCGAGACAA GTGGACCAAA GGCAGCCCGG ATCAAGAGAA CACAATAGGA 
GGAGCTCCAG AAAACCTGAC CTTCAAGGAG CGCCAGAGAC TCTTCTCGCA GGGGAAAGAG 
GTCTCCAATA AAGTCAAAGC GTCCCGAAAG CTGATGGAGC TGGAGAACGA ACTCAACATA 
AAACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352620.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208109.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X12, mRNA.

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CloneID	ODa42833
Clone ID Related Accession (Same CDS sequence)	XM_017352620.1
Accession Version	XM_017352620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGAAACA GAAGAACATC 
GCTGGAGCTC TGGCGTTTTG GATGGCGAAC GCTTCTGAGC TGCTGAACTT CATCAAACAG 
GACAAGGATC TGAGTCGCAT CACGCTGGAT GCTCAGGATG TGCTCGCTCA TCTTGTTCAG 
ATGGCCTTCA AGTACCTGGT GCACTGTCTG CAGTCAGATC TCAATAACTA CATGCCAGCT 
TTCCTGGAAG ATCCCGACGA GCAAAACCCA CAGAGGCCTA AGATAGAGGA TGTTCTGCAC 
ACGCTGACGG GTGCCATGTC TCTGCTGCGC CGATGCCGTG TGAACGCGGC GCTCACCATC 
CAACTGTTTT CGCAGCTCTT CCACTTCGTC AACATGTGGC TCTTTAACAA GCTGGTGACG 
GAGGCCGATT CTGGCCTGTG CTCACACTAC TGGGGCGCCA TCCTTCGCCA GCAGCTCAGT 
CATATTGAGG CCTGGGCTGA GAAACAAGGC TTGGAGCTGG CTGCAGACTG CCACCTCAGC 
CGCATAGTCC AGGCGACGAC CCTCCTTACT ATGGACAAGT ACTCCATGCA AGATGTCCAG 
AACATCAACA ACACCTGCTT CAAGCTCAAC TCTCTTCAGC TCAACGCTCT GCTGAGCAAC 
TACCACTGCG CTCCTGACGA ACCCTACATT CCTCCTGAGC TGATTGATCA CGTGGTGGCC 
GTGGCCGAAA ACACTGCAGA CGAACTGGCC CGAAGCGACG GGCGGGAGGT TCAGCTGGAA 
GAGGACCCCG ACCTTCAGCT GCCTTTCTTG CTGCCTGAGG ACGGCTACTC GTGCGATGTG 
GTGCGCAACA TTCCCAACGG ATTCCAAGAG TTTCTAGAGC CACTTTGCCA GAGAGGTTTC 
TGTAGATTGA CTCCTCATCC ACGCTCGCCG GGAACTTGGA CCATTTATTT TGAAGGAGCC 
AACTGTGACA GTCACTTCTC AGCCGACACC TCAGAACTGC CAATGCGGAA GGAACCGGAA 
ATTGTGACCG TGACTTTGAA GAAACATAAT GGAATGGGCC TCAGTATTGT GGCGGCCAAG 
GGTGCCGGGC AGGACAAACT TGGTATTTAC ATCAAATCTG TTGTCAAAGG AGGTGCAGCA 
GACATGGATG GGCGTCTGGC TGCTGGAGAC CAGTTGCTTA GCGTGGATGG ACGTAGTCTT 
GTCGGCCTCT CGCAGGAGAG AGCGGCAGAA TTGATGACAC GAACTGGGTC TGTTGTAACG 
CTTGAGGTGG CCAAACAAGG AGCCATTTAT CACGGTCTGG CCACACTTCT TAACCAGCCA 
TCCCCCATGA TGCCTCGCGC GTCAGACCGT GGACGTGGCG ACAAAGGAAA GCTGAGACCA 
AAGAGTGAGG GCTTTGAGCT CTACAACAAC TCTGTACAGA ACGGCTCACC TGAGAGCCCA 
CAGGGCACGT GGGACACCTA TCCTGAACCA AAGAAAGAGG ACAGGTTGTT GAAGAACCGA 
GCAGACCATC GATCCAGCCC AAATGTGGCC AATCAGGCTC AGAGTCCAGG CGGGAAGCCT 
GTTTATCCGG GAGCTCCAGG AAAGATCACT TCGGTCTCGA CGGGTAACCT CTGCCCTGAT 
GAGGAGCCCT CTCCACCCCG TCCAGAGGCC TATCCCATCC CCACTCAGAC TTACCCTAGG 
GAATACTTCA CCTTCCCAGC CTCCAAATCT CAGGACAGGA TGGGCCCTGG CCAGCCCTGG 
CAAAACAACG AACAAGAGCC TCTTCCCCCT ATGGATAACC ACTCCAACAA CAGCATGGTT 
ATGCAGCGGG TGGCGCGATC TCAGGAGGAG CTGTGTGACA TGCCGGGCGG GTACCCAGGT 
GAACGTCTGC GCCAGGAAGA TCTAATGCTC CTCCAGCAGC AGCAGCAGCG TGAAGCTGAG 
TACCAGCGAG GTGTTGGAGA ACACTGGAAC CACCAGGTGT CTTCCTCAGT GGAGTCCAGC 
ACCTCCAGCC AAGAGCACCT CAACTTCTCC TCATCCTCTG GCTCCAGGAC CCAGGGCAAC 
CACAAGAGTG GCCCAGGCCG CTGGAAGACC CCACTTGCAC CTCATTCCGG CCCTGTCTCC 
CAACCCTCCC GTTCAGACCT TCCTCCACCT CCACCTCCTC CACCAGCACA CTACGATTTT 
GAGCCGCAAA CCGACCTGCC GCTTCCACCG CCACCTTCAG CTACACAACA ACAGCAGCAG 
GCTGCAGCCG ACCGCAAGAA ACGTGAAGAC CAACAGCGCT GGTATGAGAA GGAAAAAGCT 
CGTCTTGAAG AGGAGCGAGA GAGGAAGAGA CGAGATCAGG AAAGGAAATT GGTGCAAATC 
CGAAACCCTT CGGTTTCTGG TCCAGTTATG CACAATAACC AGCATGGCCC GGTGCCACCA 
TCTCCACAAA TGCCTCCCAT GACACTTCCC CAATCATACC TTCCTCCAGT CCAACAACCT 
CCGCCACCAC AACCGGCTCG ACCCGATAAA CTCTCCAGTC TTCCTCGCCA GCCTCCGCAA 
GACACCGTAA TCCGGGACCT CCTGCCGCAG CAGCAGCCGC GCACCATCGA GAGACGCGAC 
CTGCAGTACA TCACCATCAG CAAGGAGGAG TTATCGGCCA GCGACAGCCT GTCACCCGAC 
CCCTGGAAGA GGGACGCCCG GGAAAAACTG GAGAAGCAGC AGCAGCTTCA CATCGTTGAC 
CTGCTGGACA AGGAGATCCA GGAGCTGCAG GCTAAACCCG AGCGCACGGC CGAGGACAAC 
GACCGCCTGC GCAAACTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTGCA GGAGTCCAAG 
CAGAACGAGG AGGACGAGGA GGAGGAAGAC GATGAAGACG TGGACACCAC GCTCATCATG 
CAGAGACTGG AGGCCGAGAA GAGAGCACGG CAGACTGCTG TTCCAGCAAT CTCTGTACTA 
GACTTGCTGC AGGACGAGGA GCGACGACGG AAGCAGCAGC TGGAGGAAAT CCGCAAGCGC 
GAAGCAGAAG AGCGAGCCAA ACAGGAGGAG GAGAGACGCT GGAGAGAGGA GGAGAGAGCC 
AGGAGAGAGG CTGACGAGAA GAGGAGACAG GAGGAAGAGT ACTACACCCG CCTGGAGGCA 
GAGCGGCGCC GGCAGCACGA GGAAGCTGAG CGCAAGCTGC CGACGCCAGA TGAGACGGGT 
CTGTACAGAC CCCCTTTACC CCGGGACTAC CAGCCCCCTT CTCCCTCAAG CGCACCCGCC 
ACTAACCACA CCACCAGCGC CCCCCCGCCT CCGCCTCAGA GAAACACCTC CTACCTCAAA 
ACCCAGGTCG CCTCCCCAGA CACCATTTAC ACTGCCAAGT TTGTCTCGTA CAATGACGAG 
GAGGAGGAGG AGGATGAGGA AGATGCGGGC CTAACAGGAT TTAATTCATA CACTGGAAAC 
ACAACAGGAC TAGTATCAGG AGAGGTGTAT AAAGACCCGC GAGACAAGTG GACCAAAGGC 
CCGGATCAAG AGAACACAAT AGGAGGAGCT CCAGAAAACC TGACCTTCAA GGAGCGCCAG 
AGACTCTTCT CGCAGGGGAA AGAGGTCTCC AATAAAGTCA AAGCGTCCCG AAAGCTGATG 
GAGCTGGAGA ACGAACTCAA CATAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208109.1
CDS	34..5766
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPGRGSHYAYYYRHHEDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352620.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352620.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AAGAGAGGAA ACTGGACTTG GATGAGAAAC CACTGGTCGT GCAGCTGAAC 
TGGAATAAAG ACGACAGAGA GGGCCGCTTC GTTCTCAAGA ACGAGAATGA CATTATGCCC 
AAGAGAACAC TGACAAACGG TCCTGAAAAG GAGAAAGAGG GGGTGATCCA GAACTTCAAA 
AGAACACTGT CCAAGAAGGA GAAGAAGAAA GAGAAGAAGA GGGAGAAGGA GGCTTTTTCC 
AGAATCCCAG ATGGAGACGA TCATGTGTTG AACCGCGAGG ATGGAGAGAA CTCCAGATTA 
GCAGCTGAGG TTTATAAAGA CATGCCGGAG ACCAGTTTCA CAAGGACCAT TTCTAACCCC 
GAGGTGGTGA TGAAGAGAAG ACGACAGCAG AAGCTGGAGA AGAGGATGCA GGAGTTTCGG 
AGTGAAGACG GTCGACCCGA CTCAGGAGGC ACTCTGAGGA TCTACGCTGA TAGTCTGAAG 
CCCAACATTC CCTACAAGAC CATCCTGCTC TCAACTACTG ACATGGCTGA CTTTGCTGTG 
GCCGAGGCTC TGGAGAAATA TGGCCTGGAG AAGGAAAACC CTAGAGACTA CTGCATCGCC 
CAGGTGATTC ATGCAAATGA TAAAGCAGTG AAGGAGACCA TATTAGATGA CACAGACTGT 
CCTCTGCAGA TCCTCAGAGA CTGGACGAGT GATAAAGAGG CCTTAGTTTT CCAGCTGAAG 
AAGCGTCCTC CAGATTACGC TCCAAGGAAA GGACGTAAGC CAGACAACCG GATGGGGCCG 
CAGCACGGAT CCATTCCTCC TGAGAAACTG CCTTACCTGG TGGAGCTGAG CCCAGGGCGA 
GGGAGTCACT ATGCCTACTA CTACCGCCAT CATGAAGATG GCTCCGACTC CAGGGACAAA 
CCCAAACTCT ACAGACTACA GCACAGCGTT ACTGAGGTCG GATCTGACCG GAGTGAAGAT 
GGAAACATCC AGCTGTTTGG GCCTGGGATT CTCCCTCATC ACTGTGACCT TATGCATGCG 
GACGGCTTGG TGACCATAAC CCCAGCCAAT ATGGACGCAG AGACATTTGT GGATGGACAG 
CGGATCGCTG ACACTACAGT TCTGCGCAGT GGGGTGACCG TCCAGTTCGG AGCAACTCAC 
GTCTTCAAAT TCGTAGACCC CAGTTATGAC CAGAGCATGT CCAAAAGAGA CCCCGGGCCC 
ATGATGAAAG GCAGGCACAA ATCAGGGAGT GTCCCAGAGA CGACCTTTGA CCTCCACGGG 
GACATTCACA GTGGCTCTGC TCTCCCTACA AGCAAGAGCT CAGGGAAGCT GGAGATGGAG 
CGGTCAGGAA ACAGCAGTGA AAGAGGCCTG GTGAAGCCCA TGATTCGTGG AGAGCAGCAG 
GACTTTAGCA AGCATGATGG AAGGTCTCAG GACGGCCCGG AGCTCACTCT GCCCGCCAGC 
ATCGAGTTCA GGGAAAACTC CGAGGATGCC TTCCTGTCGG CCATCATCAA CTACACCAAC 
AGCTCGACAG TGCACTTCAA GCTTTCTCCC ACATACGTGC TGTACATGAC CTGCCGTTAT 
GTCATGTCCA GCCAATACCG GCCCGACATC AGTCCCTCCG AACGCACACA CAAAGTCATC 
GCCATTGTCA ACAAGATGGT GAGCATGATG GAAGGAGTCA TCCAGAAACA GAAGAACATC 
GCTGGAGCTC TGGCGTTTTG GATGGCGAAC GCTTCTGAGC TGCTGAACTT CATCAAACAG 
GACAAGGATC TGAGTCGCAT CACGCTGGAT GCTCAGGATG TGCTCGCTCA TCTTGTTCAG 
ATGGCCTTCA AGTACCTGGT GCACTGTCTG CAGTCAGATC TCAATAACTA CATGCCAGCT 
TTCCTGGAAG ATCCCGACGA GCAAAACCCA CAGAGGCCTA AGATAGAGGA TGTTCTGCAC 
ACGCTGACGG GTGCCATGTC TCTGCTGCGC CGATGCCGTG TGAACGCGGC GCTCACCATC 
CAACTGTTTT CGCAGCTCTT CCACTTCGTC AACATGTGGC TCTTTAACAA GCTGGTGACG 
GAGGCCGATT CTGGCCTGTG CTCACACTAC TGGGGCGCCA TCCTTCGCCA GCAGCTCAGT 
CATATTGAGG CCTGGGCTGA GAAACAAGGC TTGGAGCTGG CTGCAGACTG CCACCTCAGC 
CGCATAGTCC AGGCGACGAC CCTCCTTACT ATGGACAAGT ACTCCATGCA AGATGTCCAG 
AACATCAACA ACACCTGCTT CAAGCTCAAC TCTCTTCAGC TCAACGCTCT GCTGAGCAAC 
TACCACTGCG CTCCTGACGA ACCCTACATT CCTCCTGAGC TGATTGATCA CGTGGTGGCC 
GTGGCCGAAA ACACTGCAGA CGAACTGGCC CGAAGCGACG GGCGGGAGGT TCAGCTGGAA 
GAGGACCCCG ACCTTCAGCT GCCTTTCTTG CTGCCTGAGG ACGGCTACTC GTGCGATGTG 
GTGCGCAACA TTCCCAACGG ATTCCAAGAG TTTCTAGAGC CACTTTGCCA GAGAGGTTTC 
TGTAGATTGA CTCCTCATCC ACGCTCGCCG GGAACTTGGA CCATTTATTT TGAAGGAGCC 
AACTGTGACA GTCACTTCTC AGCCGACACC TCAGAACTGC CAATGCGGAA GGAACCGGAA 
ATTGTGACCG TGACTTTGAA GAAACATAAT GGAATGGGCC TCAGTATTGT GGCGGCCAAG 
GGTGCCGGGC AGGACAAACT TGGTATTTAC ATCAAATCTG TTGTCAAAGG AGGTGCAGCA 
GACATGGATG GGCGTCTGGC TGCTGGAGAC CAGTTGCTTA GCGTGGATGG ACGTAGTCTT 
GTCGGCCTCT CGCAGGAGAG AGCGGCAGAA TTGATGACAC GAACTGGGTC TGTTGTAACG 
CTTGAGGTGG CCAAACAAGG AGCCATTTAT CACGGTCTGG CCACACTTCT TAACCAGCCA 
TCCCCCATGA TGCCTCGCGC GTCAGACCGT GGACGTGGCG ACAAAGGAAA GCTGAGACCA 
AAGAGTGAGG GCTTTGAGCT CTACAACAAC TCTGTACAGA ACGGCTCACC TGAGAGCCCA 
CAGGGCACGT GGGACACCTA TCCTGAACCA AAGAAAGAGG ACAGGTTGTT GAAGAACCGA 
GCAGACCATC GATCCAGCCC AAATGTGGCC AATCAGGCTC AGAGTCCAGG CGGGAAGCCT 
GTTTATCCGG GAGCTCCAGG AAAGATCACT TCGGTCTCGA CGGGTAACCT CTGCCCTGAT 
GAGGAGCCCT CTCCACCCCG TCCAGAGGCC TATCCCATCC CCACTCAGAC TTACCCTAGG 
GAATACTTCA CCTTCCCAGC CTCCAAATCT CAGGACAGGA TGGGCCCTGG CCAGCCCTGG 
CAAAACAACG AACAAGAGCC TCTTCCCCCT ATGGATAACC ACTCCAACAA CAGCATGGTT 
ATGCAGCGGG TGGCGCGATC TCAGGAGGAG CTGTGTGACA TGCCGGGCGG GTACCCAGGT 
GAACGTCTGC GCCAGGAAGA TCTAATGCTC CTCCAGCAGC AGCAGCAGCG TGAAGCTGAG 
TACCAGCGAG GTGTTGGAGA ACACTGGAAC CACCAGGTGT CTTCCTCAGT GGAGTCCAGC 
ACCTCCAGCC AAGAGCACCT CAACTTCTCC TCATCCTCTG GCTCCAGGAC CCAGGGCAAC 
CACAAGAGTG GCCCAGGCCG CTGGAAGACC CCACTTGCAC CTCATTCCGG CCCTGTCTCC 
CAACCCTCCC GTTCAGACCT TCCTCCACCT CCACCTCCTC CACCAGCACA CTACGATTTT 
GAGCCGCAAA CCGACCTGCC GCTTCCACCG CCACCTTCAG CTACACAACA ACAGCAGCAG 
GCTGCAGCCG ACCGCAAGAA ACGTGAAGAC CAACAGCGCT GGTATGAGAA GGAAAAAGCT 
CGTCTTGAAG AGGAGCGAGA GAGGAAGAGA CGAGATCAGG AAAGGAAATT GGTGCAAATC 
CGAAACCCTT CGGTTTCTGG TCCAGTTATG CACAATAACC AGCATGGCCC GGTGCCACCA 
TCTCCACAAA TGCCTCCCAT GACACTTCCC CAATCATACC TTCCTCCAGT CCAACAACCT 
CCGCCACCAC AACCGGCTCG ACCCGATAAA CTCTCCAGTC TTCCTCGCCA GCCTCCGCAA 
GACACCGTAA TCCGGGACCT CCTGCCGCAG CAGCAGCCGC GCACCATCGA GAGACGCGAC 
CTGCAGTACA TCACCATCAG CAAGGAGGAG TTATCGGCCA GCGACAGCCT GTCACCCGAC 
CCCTGGAAGA GGGACGCCCG GGAAAAACTG GAGAAGCAGC AGCAGCTTCA CATCGTTGAC 
CTGCTGGACA AGGAGATCCA GGAGCTGCAG GCTAAACCCG AGCGCACGGC CGAGGACAAC 
GACCGCCTGC GCAAACTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTGCA GGAGTCCAAG 
CAGAACGAGG AGGACGAGGA GGAGGAAGAC GATGAAGACG TGGACACCAC GCTCATCATG 
CAGAGACTGG AGGCCGAGAA GAGAGCACGG CAGACTGCTG TTCCAGCAAT CTCTGTACTA 
GACTTGCTGC AGGACGAGGA GCGACGACGG AAGCAGCAGC TGGAGGAAAT CCGCAAGCGC 
GAAGCAGAAG AGCGAGCCAA ACAGGAGGAG GAGAGACGCT GGAGAGAGGA GGAGAGAGCC 
AGGAGAGAGG CTGACGAGAA GAGGAGACAG GAGGAAGAGT ACTACACCCG CCTGGAGGCA 
GAGCGGCGCC GGCAGCACGA GGAAGCTGAG CGCAAGCTGC CGACGCCAGA TGAGACGGGT 
CTGTACAGAC CCCCTTTACC CCGGGACTAC CAGCCCCCTT CTCCCTCAAG CGCACCCGCC 
ACTAACCACA CCACCAGCGC CCCCCCGCCT CCGCCTCAGA GAAACACCTC CTACCTCAAA 
ACCCAGGTCG CCTCCCCAGA CACCATTTAC ACTGCCAAGT TTGTCTCGTA CAATGACGAG 
GAGGAGGAGG AGGATGAGGA AGATGCGGGC CTAACAGGAT TTAATTCATA CACTGGAAAC 
ACAACAGGAC TAGTATCAGG AGAGGTGTAT AAAGACCCGC GAGACAAGTG GACCAAAGGC 
CCGGATCAAG AGAACACAAT AGGAGGAGCT CCAGAAAACC TGACCTTCAA GGAGCGCCAG 
AGACTCTTCT CGCAGGGGAA AGAGGTCTCC AATAAAGTCA AAGCGTCCCG AAAGCTGATG 
GAGCTGGAGA ACGAACTCAA CATAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mllt4a ( XM_017352621.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208110.1 Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X13, mRNA.

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CloneID	ODa42834
Clone ID Related Accession (Same CDS sequence)	XM_017352621.1
Accession Version	XM_017352621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	afadin isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AGAGGAAACT GGACTTGGAT GAGAAACCAC TGGTCGTGCA GCTGAACTGG 
AATAAAGACG ACAGAGAGGG CCGCTTCGTT CTCAAGAACG AGAATGACAT TATGCCCAAG 
AGAACACTGA CAAACGGTCC TGAAAAGGAG AAAGAGGGGG TGATCCAGAA CTTCAAAAGA 
ACACTGTCCA AGAAGGAGAA GAAGAAAGAG AAGAAGAGGG AGAAGGAGGC TTTTTCCAGA 
ATCCCAGATG GAGACGATCA TGTGTTGAAC CGCGAGGATG GAGAGAACTC CAGATTAGCA 
GCTGAGGTTT ATAAAGACAT GCCGGAGACC AGTTTCACAA GGACCATTTC TAACCCCGAG 
GTGGTGATGA AGAGAAGACG ACAGCAGAAG CTGGAGAAGA GGATGCAGGA GTTTCGGAGT 
GAAGACGGTC GACCCGACTC AGGAGGCACT CTGAGGATCT ACGCTGATAG TCTGAAGCCC 
AACATTCCCT ACAAGACCAT CCTGCTCTCA ACTACTGACA TGGCTGACTT TGCTGTGGCC 
GAGGCTCTGG AGAAATATGG CCTGGAGAAG GAAAACCCTA GAGACTACTG CATCGCCCAG 
GTGATTCATG CAAATGATAA AGCAGTGAAG GAGACCATAT TAGATGACAC AGACTGTCCT 
CTGCAGATCC TCAGAGACTG GACGAGTGAT AAAGAGGCCT TAGTTTTCCA GCTGAAGAAG 
CGTCCTCCAG ATTACGCTCC AAGGAAAGGA CGTAAGCCAG ACAACCGGAT GGGGCCGCAG 
CACGGATCCA TTCCTCCTGA GAAACTGCCT TACCTGGTGG AGCTGAGCCC AGATGGCTCC 
GACTCCAGGG ACAAACCCAA ACTCTACAGA CTACAGCACA GCGTTACTGA GGTCGGATCT 
GACCGGAGTG AAGATGGAAA CATCCAGCTG TTTGGGCCTG GGATTCTCCC TCATCACTGT 
GACCTTATGC ATGCGGACGG CTTGGTGACC ATAACCCCAG CCAATATGGA CGCAGAGACA 
TTTGTGGATG GACAGCGGAT CGCTGACACT ACAGTTCTGC GCAGTGGGGT GACCGTCCAG 
TTCGGAGCAA CTCACGTCTT CAAATTCGTA GACCCCAGTT ATGACCAGAG CATGTCCAAA 
AGAGACCCCG GGCCCATGAT GAAAGGCAGG CACAAATCAG GGAGTGTCCC AGAGACGACC 
TTTGACCTCC ACGGGGACAT TCACAGTGGC TCTGCTCTCC CTACAAGCAA GAGCTCAGGG 
AAGCTGGAGA TGGAGCGGTC AGGAAACAGC AGTGAAAGAG GCCTGGTGAA GCCCATGATT 
CGTGGAGAGC AGCAGGACTT TAGCAAGCAT GATGGAAGGT CTCAGGACGG CCCGGAGCTC 
ACTCTGCCCG CCAGCATCGA GTTCAGGGAA AACTCCGAGG ATGCCTTCCT GTCGGCCATC 
ATCAACTACA CCAACAGCTC GACAGTGCAC TTCAAGCTTT CTCCCACATA CGTGCTGTAC 
ATGACCTGCC GTTATGTCAT GTCCAGCCAA TACCGGCCCG ACATCAGTCC CTCCGAACGC 
ACACACAAAG TCATCGCCAT TGTCAACAAG ATGGTGAGCA TGATGGAAGG AGTCATCCAG 
AAACAGAAGA ACATCGCTGG AGCTCTGGCG TTTTGGATGG CGAACGCTTC TGAGCTGCTG 
AACTTCATCA AACAGGACAA GGATCTGAGT CGCATCACGC TGGATGCTCA GGATGTGCTC 
GCTCATCTTG TTCAGATGGC CTTCAAGTAC CTGGTGCACT GTCTGCAGTC AGATCTCAAT 
AACTACATGC CAGCTTTCCT GGAAGATCCC GACGAGCAAA ACCCACAGAG GCCTAAGATA 
GAGGATGTTC TGCACACGCT GACGGGTGCC ATGTCTCTGC TGCGCCGATG CCGTGTGAAC 
GCGGCGCTCA CCATCCAACT GTTTTCGCAG CTCTTCCACT TCGTCAACAT GTGGCTCTTT 
AACAAGCTGG TGACGGAGGC CGATTCTGGC CTGTGCTCAC ACTACTGGGG CGCCATCCTT 
CGCCAGCAGC TCAGTCATAT TGAGGCCTGG GCTGAGAAAC AAGGCTTGGA GCTGGCTGCA 
GACTGCCACC TCAGCCGCAT AGTCCAGGCG ACGACCCTCC TTACTATGGA CAAGTACTCC 
ATGCAAGATG TCCAGAACAT CAACAACACC TGCTTCAAGC TCAACTCTCT TCAGCTCAAC 
GCTCTGCTGA GCAACTACCA CTGCGCTCCT GACGAACCCT ACATTCCTCC TGAGCTGATT 
GATCACGTGG TGGCCGTGGC CGAAAACACT GCAGACGAAC TGGCCCGAAG CGACGGGCGG 
GAGGTTCAGC TGGAAGAGGA CCCCGACCTT CAGCTGCCTT TCTTGCTGCC TGAGGACGGC 
TACTCGTGCG ATGTGGTGCG CAACATTCCC AACGGATTCC AAGAGTTTCT AGAGCCACTT 
TGCCAGAGAG GTTTCTGTAG ATTGACTCCT CATCCACGCT CGCCGGGAAC TTGGACCATT 
TATTTTGAAG GAGCCAACTG TGACAGTCAC TTCTCAGCCG ACACCTCAGA ACTGCCAATG 
CGGAAGGAAC CGGAAATTGT GACCGTGACT TTGAAGAAAC ATAATGGAAT GGGCCTCAGT 
ATTGTGGCGG CCAAGGGTGC CGGGCAGGAC AAACTTGGTA TTTACATCAA ATCTGTTGTC 
AAAGGAGGTG CAGCAGACAT GGATGGGCGT CTGGCTGCTG GAGACCAGTT GCTTAGCGTG 
GATGGACGTA GTCTTGTCGG CCTCTCGCAG GAGAGAGCGG CAGAATTGAT GACACGAACT 
GGGTCTGTTG TAACGCTTGA GGTGGCCAAA CAAGGAGCCA TTTATCACGG TCTGGCCACA 
CTTCTTAACC AGCCATCCCC CATGATGCCT CGCGCGTCAG ACCGTGGACG TGGCGACAAA 
GGAAAGCTGA GACCAAAGAG TGAGGGCTTT GAGCTCTACA ACAACTCTGT ACAGAACGGC 
TCACCTGAGA GCCCACAGGG CACGTGGGAC ACCTATCCTG AACCAAAGAA AGAGGACAGG 
TTGTTGAAGA ACCGAGCAGA CCATCGATCC AGCCCAAATG TGGCCAATCA GGCTCAGAGT 
CCAGGCGGGA AGCCTGTTTA TCCGGGAGCT CCAGGAAAGA TCACTTCGGT CTCGACGGGT 
AACCTCTGCC CTGATGAGGA GCCCTCTCCA CCCCGTCCAG AGGCCTATCC CATCCCCACT 
CAGACTTACC CTAGGGAATA CTTCACCTTC CCAGCCTCCA AATCTCAGGA CAGGATGGGC 
CCTGGCCAGC CCTGGCAAAA CAACGAACAA GAGCCTCTTC CCCCTATGGA TAACCACTCC 
AACAACAGCA TGGTTATGCA GCGGGTGGCG CGATCTCAGG AGGAGCTGTG TGACATGCCG 
GGCGGGTACC CAGGTGAACG TCTGCGCCAG GAAGATCTAA TGCTCCTCCA GCAGCAGCAG 
CAGCGTGAAG CTGAGTACCA GCGAGGTGTT GGAGAACACT GGAACCACCA GGTGTCTTCC 
TCAGTGGAGT CCAGCACCTC CAGCCAAGAG CACCTCAACT TCTCCTCATC CTCTGGCTCC 
AGGACCCAGG GCAACCACAA GAGTGGCCCA GGCCGCTGGA AGACCCCACT TGCACCTCAT 
TCCGGCCCTG TCTCCCAACC CTCCCGTTCA GACCTTCCTC CACCTCCACC TCCTCCACCA 
GCACACTACG ATTTTGAGCC GCAAACCGAC CTGCCGCTTC CACCGCCACC TTCAGCTACA 
CAACAACAGC AGCAGGCTGC AGCCGACCGC AAGAAACGTG AAGACCAACA GCGCTGGTAT 
GAGAAGGAAA AAGCTCGTCT TGAAGAGGAG CGAGAGAGGA AGAGACGAGA TCAGGAAAGG 
AAATTGGTGC AAATCCGAAA CCCTTCGGTT TCTGGTCCAG TTATGCACAA TAACCAGCAT 
GGCCCGGTGC CACCATCTCC ACAAATGCCT CCCATGACAC TTCCCCAATC ATACCTTCCT 
CCAGTCCAAC AACCTCCGCC ACCACAACCG GCTCGACCCG ATAAACTCTC CAGTCTTCCT 
CGCCAGCCTC CGCAAGACAC CGTAATCCGG GACCTCCTGC CGCAGCAGCA GCCGCGCACC 
ATCGAGAGAC GCGACCTGCA GTACATCACC ATCAGCAAGG AGGAGTTATC GGCCAGCGAC 
AGCCTGTCAC CCGACCCCTG GAAGAGGGAC GCCCGGGAAA AACTGGAGAA GCAGCAGCAG 
CTTCACATCG TTGACCTGCT GGACAAGGAG ATCCAGGAGC TGCAGGCTAA ACCCGAGCGC 
ACGGCCGAGG ACAACGACCG CCTGCGCAAA CTCATGCTGG AGTGGCAGTT CCAGAAGAGA 
CTGCAGGAGT CCAAGCAGAA CGAGGAGGAC GAGGAGGAGG AAGACGATGA AGACGTGGAC 
ACCACGCTCA TCATGCAGAG ACTGGAGGCC GAGAAGAGAG CACGGCTGCA GGACGAGGAG 
CGACGACGGA AGCAGCAGCT GGAGGAAATC CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA 
CAGGAGGAGG AGAGACGCTG GAGAGAGGAG GAGAGAGCCA GGAGAGAGGC TGACGAGAAG 
AGGAGACAGG AGGAAGAGTA CTACACCCGC CTGGAGGCAG AGCGGCGCCG GCAGCACGAG 
GAAGCTGAGC GCAAGCTGCC GACGCCAGAT GAGACGGGTC TGTACAGACC CCCTTTACCC 
CGGGACTACC AGCCCCCTTC TCCCTCAAGC GCACCCGCCA CTAACCACAC CACCAGCGCC 
CCCCCGCCTC CGCCTCAGAG AAACACCTCC TACCTCAAAA CCCAGGTCGC CTCCCCAGAC 
ACCATTTACA CTGCCAAGTT TGTCTCGTAC AATGACGAGG AGGAGGAGGA GGATGAGGAA 
GATGCGGGCC TAACAGGTCA GGTTAAGCTC TCGGCCACCC GCAAGTCCTA CGGCGACCTT 
CCGCCTGCCC CAAAGCCCCA GCCGCCGCCA ACCGCGCGCA AGCCCCGTCC CGTCTCAGAT 
GGCATCTTTC TGTCCAACTC ATTCCAGCTG CCCGCTGGGC TCAATGCCAA CAGTACTGCT 
CTCAAGACAG GGCAGCCCCC TCTCCCCTTC ACCAAACCCA GCTTCATCCC ATCAGCCAAC 
TCCAAAGGAT TTAATTCATA CACTGGAAAC ACAACAGGAC TAGTATCAGG AGAGGTGTAT 
AAAGACCCGC GAGACAAGTG GACCAAAGGC CCGGATCAAG AGAACACAAT AGGAGGAGCT 
CCAGAAAACC TGACCTTCAA GGAGCGCCAG AGACTCTTCT CGCAGGGGAA AGAGGTCTCC 
AATAAAGTCA AAGCGTCCCG AAAGCTGATG GAGCTGGAGA ACGAACTCAA CATAAAACAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208110.1
CDS	32..5914
Translation	MKTGHVHTTRREPTLGERRRHCGYTWNRQGDRIRNPFEWITPNQRRKRRIVSPSDLASISLDLRMSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVKETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRKPDNRMGPQHGSIPPEKLPYLVELSPDGSDSRDKPKLYRLQHSVTEVGSDRSEDGNIQLFGPGILPHHCDLMHADGLVTITPANMDAETFVDGQRIADTTVLRSGVTVQFGATHVFKFVDPSYDQSMSKRDPGPMMKGRHKSGSVPETTFDLHGDIHSGSALPTSKSSGKLEMERSGNSSERGLVKPMIRGEQQDFSKHDGRSQDGPELTLPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFLEDPDEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLEPLCQRGFCRLTPHPRSPGTWTIYFEGANCDSHFSADTSELPMRKEPEIVTVTLKKHNGMGLSIVAAKGAGQDKLGIYIKSVVKGGAADMDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRGDKGKLRPKSEGFELYNNSVQNGSPESPQGTWDTYPEPKKEDRLLKNRADHRSSPNVANQAQSPGGKPVYPGAPGKITSVSTGNLCPDEEPSPPRPEAYPIPTQTYPREYFTFPASKSQDRMGPGQPWQNNEQEPLPPMDNHSNNSMVMQRVARSQEELCDMPGGYPGERLRQEDLMLLQQQQQREAEYQRGVGEHWNHQVSSSVESSTSSQEHLNFSSSSGSRTQGNHKSGPGRWKTPLAPHSGPVSQPSRSDLPPPPPPPPAHYDFEPQTDLPLPPPPSATQQQQQAAADRKKREDQQRWYEKEKARLEEERERKRRDQERKLVQIRNPSVSGPVMHNNQHGPVPPSPQMPPMTLPQSYLPPVQQPPPPQPARPDKLSSLPRQPPQDTVIRDLLPQQQPRTIERRDLQYITISKEELSASDSLSPDPWKRDAREKLEKQQQLHIVDLLDKEIQELQAKPERTAEDNDRLRKLMLEWQFQKRLQESKQNEEDEEEEDDEDVDTTLIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERARREADEKRRQEEEYYTRLEAERRRQHEEAERKLPTPDETGLYRPPLPRDYQPPSPSSAPATNHTTSAPPPPPQRNTSYLKTQVASPDTIYTAKFVSYNDEEEEEDEEDAGLTGQVKLSATRKSYGDLPPAPKPQPPPTARKPRPVSDGIFLSNSFQLPAGLNANSTALKTGQPPLPFTKPSFIPSANSKGFNSYTGNTTGLVSGEVYKDPRDKWTKGPDQENTIGGAPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNIKQ

Target ORF information:

RefSeq Version	XM_017352621.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a (mllt4a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352621.1

ORF Insert Sequence:

ATGAAAACGG GACACGTACA CACAACCAGG AGGGAACCAA CACTAGGAGA GAGACGAAGA 
CACTGTGGGT ATACTTGGAA CAGACAAGGT GATCGGATAA GAAACCCGTT TGAGTGGATA 
ACACCGAACC AGCGAAGGAA AAGGCGGATT GTTTCTCCCT CCGACTTGGC TAGTATTAGT 
CTGGATTTGA GAATGTCGGG GAGCCGAGAG GAGGAGCGGC GGAAACTCGC CGACATCATC 
AACCACTGGA ACGCAAACCG GCTCGACCTG TTCGAGATCA GCCAACCCAC AGAGGATTTG 
GAGTTCCATG GGGTGATGCG GTTTTACTTC CAGGACCGTG TGGCAGGGAA TTTTGCCACA 
AAGTGCATCA GAGTGTCCAG TACAGCCACC ACACAAGATG TGATCGAGAC GCTGGCGGAG 
AAGTTCAGAC CCGACATGAG GATGCTGTCT TCTCCAAAAT ACTCCCTCTA TGAGGTCCAC 
GTCAGTGGCG AGAGGAAACT GGACTTGGAT GAGAAACCAC TGGTCGTGCA GCTGAACTGG 
AATAAAGACG ACAGAGAGGG CCGCTTCGTT CTCAAGAACG AGAATGACAT TATGCCCAAG 
AGAACACTGA CAAACGGTCC TGAAAAGGAG AAAGAGGGGG TGATCCAGAA CTTCAAAAGA 
ACACTGTCCA AGAAGGAGAA GAAGAAAGAG AAGAAGAGGG AGAAGGAGGC TTTTTCCAGA 
ATCCCAGATG GAGACGATCA TGTGTTGAAC CGCGAGGATG GAGAGAACTC CAGATTAGCA 
GCTGAGGTTT ATAAAGACAT GCCGGAGACC AGTTTCACAA GGACCATTTC TAACCCCGAG 
GTGGTGATGA AGAGAAGACG ACAGCAGAAG CTGGAGAAGA GGATGCAGGA GTTTCGGAGT 
GAAGACGGTC GACCCGACTC AGGAGGCACT CTGAGGATCT ACGCTGATAG TCTGAAGCCC 
AACATTCCCT ACAAGACCAT CCTGCTCTCA ACTACTGACA TGGCTGACTT TGCTGTGGCC 
GAGGCTCTGG AGAAATATGG CCTGGAGAAG GAAAACCCTA GAGACTACTG CATCGCCCAG 
GTGATTCATG CAAATGATAA AGCAGTGAAG GAGACCATAT TAGATGACAC AGACTGTCCT 
CTGCAGATCC TCAGAGACTG GACGAGTGAT AAAGAGGCCT TAGTTTTCCA GCTGAAGAAG 
CGTCCTCCAG ATTACGCTCC AAGGAAAGGA CGTAAGCCAG ACAACCGGAT GGGGCCGCAG 
CACGGATCCA TTCCTCCTGA GAAACTGCCT TACCTGGTGG AGCTGAGCCC AGATGGCTCC 
GACTCCAGGG ACAAACCCAA ACTCTACAGA CTACAGCACA GCGTTACTGA GGTCGGATCT 
GACCGGAGTG AAGATGGAAA CATCCAGCTG TTTGGGCCTG GGATTCTCCC TCATCACTGT 
GACCTTATGC ATGCGGACGG CTTGGTGACC ATAACCCCAG CCAATATGGA CGCAGAGACA 
TTTGTGGATG GACAGCGGAT CGCTGACACT ACAGTTCTGC GCAGTGGGGT GACCGTCCAG 
TTCGGAGCAA CTCACGTCTT CAAATTCGTA GACCCCAGTT ATGACCAGAG CATGTCCAAA 
AGAGACCCCG GGCCCATGAT GAAAGGCAGG CACAAATCAG GGAGTGTCCC AGAGACGACC 
TTTGACCTCC ACGGGGACAT TCACAGTGGC TCTGCTCTCC CTACAAGCAA GAGCTCAGGG 
AAGCTGGAGA TGGAGCGGTC AGGAAACAGC AGTGAAAGAG GCCTGGTGAA GCCCATGATT 
CGTGGAGAGC AGCAGGACTT TAGCAAGCAT GATGGAAGGT CTCAGGACGG CCCGGAGCTC 
ACTCTGCCCG CCAGCATCGA GTTCAGGGAA AACTCCGAGG ATGCCTTCCT GTCGGCCATC 
ATCAACTACA CCAACAGCTC GACAGTGCAC TTCAAGCTTT CTCCCACATA CGTGCTGTAC 
ATGACCTGCC GTTATGTCAT GTCCAGCCAA TACCGGCCCG ACATCAGTCC CTCCGAACGC 
ACACACAAAG TCATCGCCAT TGTCAACAAG ATGGTGAGCA TGATGGAAGG AGTCATCCAG 
AAACAGAAGA ACATCGCTGG AGCTCTGGCG TTTTGGATGG CGAACGCTTC TGAGCTGCTG 
AACTTCATCA AACAGGACAA GGATCTGAGT CGCATCACGC TGGATGCTCA GGATGTGCTC 
GCTCATCTTG TTCAGATGGC CTTCAAGTAC CTGGTGCACT GTCTGCAGTC AGATCTCAAT 
AACTACATGC CAGCTTTCCT GGAAGATCCC GACGAGCAAA ACCCACAGAG GCCTAAGATA 
GAGGATGTTC TGCACACGCT GACGGGTGCC ATGTCTCTGC TGCGCCGATG CCGTGTGAAC 
GCGGCGCTCA CCATCCAACT GTTTTCGCAG CTCTTCCACT TCGTCAACAT GTGGCTCTTT 
AACAAGCTGG TGACGGAGGC CGATTCTGGC CTGTGCTCAC ACTACTGGGG CGCCATCCTT 
CGCCAGCAGC TCAGTCATAT TGAGGCCTGG GCTGAGAAAC AAGGCTTGGA GCTGGCTGCA 
GACTGCCACC TCAGCCGCAT AGTCCAGGCG ACGACCCTCC TTACTATGGA CAAGTACTCC 
ATGCAAGATG TCCAGAACAT CAACAACACC TGCTTCAAGC TCAACTCTCT TCAGCTCAAC 
GCTCTGCTGA GCAACTACCA CTGCGCTCCT GACGAACCCT ACATTCCTCC TGAGCTGATT 
GATCACGTGG TGGCCGTGGC CGAAAACACT GCAGACGAAC TGGCCCGAAG CGACGGGCGG 
GAGGTTCAGC TGGAAGAGGA CCCCGACCTT CAGCTGCCTT TCTTGCTGCC TGAGGACGGC 
TACTCGTGCG ATGTGGTGCG CAACATTCCC AACGGATTCC AAGAGTTTCT AGAGCCACTT 
TGCCAGAGAG GTTTCTGTAG ATTGACTCCT CATCCACGCT CGCCGGGAAC TTGGACCATT 
TATTTTGAAG GAGCCAACTG TGACAGTCAC TTCTCAGCCG ACACCTCAGA ACTGCCAATG 
CGGAAGGAAC CGGAAATTGT GACCGTGACT TTGAAGAAAC ATAATGGAAT GGGCCTCAGT 
ATTGTGGCGG CCAAGGGTGC CGGGCAGGAC AAACTTGGTA TTTACATCAA ATCTGTTGTC 
AAAGGAGGTG CAGCAGACAT GGATGGGCGT CTGGCTGCTG GAGACCAGTT GCTTAGCGTG 
GATGGACGTA GTCTTGTCGG CCTCTCGCAG GAGAGAGCGG CAGAATTGAT GACACGAACT 
GGGTCTGTTG TAACGCTTGA GGTGGCCAAA CAAGGAGCCA TTTATCACGG TCTGGCCACA 
CTTCTTAACC AGCCATCCCC CATGATGCCT CGCGCGTCAG ACCGTGGACG TGGCGACAAA 
GGAAAGCTGA GACCAAAGAG TGAGGGCTTT GAGCTCTACA ACAACTCTGT ACAGAACGGC 
TCACCTGAGA GCCCACAGGG CACGTGGGAC ACCTATCCTG AACCAAAGAA AGAGGACAGG 
TTGTTGAAGA ACCGAGCAGA CCATCGATCC AGCCCAAATG TGGCCAATCA GGCTCAGAGT 
CCAGGCGGGA AGCCTGTTTA TCCGGGAGCT CCAGGAAAGA TCACTTCGGT CTCGACGGGT 
AACCTCTGCC CTGATGAGGA GCCCTCTCCA CCCCGTCCAG AGGCCTATCC CATCCCCACT 
CAGACTTACC CTAGGGAATA CTTCACCTTC CCAGCCTCCA AATCTCAGGA CAGGATGGGC 
CCTGGCCAGC CCTGGCAAAA CAACGAACAA GAGCCTCTTC CCCCTATGGA TAACCACTCC 
AACAACAGCA TGGTTATGCA GCGGGTGGCG CGATCTCAGG AGGAGCTGTG TGACATGCCG 
GGCGGGTACC CAGGTGAACG TCTGCGCCAG GAAGATCTAA TGCTCCTCCA GCAGCAGCAG 
CAGCGTGAAG CTGAGTACCA GCGAGGTGTT GGAGAACACT GGAACCACCA GGTGTCTTCC 
TCAGTGGAGT CCAGCACCTC CAGCCAAGAG CACCTCAACT TCTCCTCATC CTCTGGCTCC 
AGGACCCAGG GCAACCACAA GAGTGGCCCA GGCCGCTGGA AGACCCCACT TGCACCTCAT 
TCCGGCCCTG TCTCCCAACC CTCCCGTTCA GACCTTCCTC CACCTCCACC TCCTCCACCA 
GCACACTACG ATTTTGAGCC GCAAACCGAC CTGCCGCTTC CACCGCCACC TTCAGCTACA 
CAACAACAGC AGCAGGCTGC AGCCGACCGC AAGAAACGTG AAGACCAACA GCGCTGGTAT 
GAGAAGGAAA AAGCTCGTCT TGAAGAGGAG CGAGAGAGGA AGAGACGAGA TCAGGAAAGG 
AAATTGGTGC AAATCCGAAA CCCTTCGGTT TCTGGTCCAG TTATGCACAA TAACCAGCAT 
GGCCCGGTGC CACCATCTCC ACAAATGCCT CCCATGACAC TTCCCCAATC ATACCTTCCT 
CCAGTCCAAC AACCTCCGCC ACCACAACCG GCTCGACCCG ATAAACTCTC CAGTCTTCCT 
CGCCAGCCTC CGCAAGACAC CGTAATCCGG GACCTCCTGC CGCAGCAGCA GCCGCGCACC 
ATCGAGAGAC GCGACCTGCA GTACATCACC ATCAGCAAGG AGGAGTTATC GGCCAGCGAC 
AGCCTGTCAC CCGACCCCTG GAAGAGGGAC GCCCGGGAAA AACTGGAGAA GCAGCAGCAG 
CTTCACATCG TTGACCTGCT GGACAAGGAG ATCCAGGAGC TGCAGGCTAA ACCCGAGCGC 
ACGGCCGAGG ACAACGACCG CCTGCGCAAA CTCATGCTGG AGTGGCAGTT CCAGAAGAGA 
CTGCAGGAGT CCAAGCAGAA CGAGGAGGAC GAGGAGGAGG AAGACGATGA AGACGTGGAC 
ACCACGCTCA TCATGCAGAG ACTGGAGGCC GAGAAGAGAG CACGGCTGCA GGACGAGGAG 
CGACGACGGA AGCAGCAGCT GGAGGAAATC CGCAAGCGCG AAGCAGAAGA GCGAGCCAAA 
CAGGAGGAGG AGAGACGCTG GAGAGAGGAG GAGAGAGCCA GGAGAGAGGC TGACGAGAAG 
AGGAGACAGG AGGAAGAGTA CTACACCCGC CTGGAGGCAG AGCGGCGCCG GCAGCACGAG 
GAAGCTGAGC GCAAGCTGCC GACGCCAGAT GAGACGGGTC TGTACAGACC CCCTTTACCC 
CGGGACTACC AGCCCCCTTC TCCCTCAAGC GCACCCGCCA CTAACCACAC CACCAGCGCC 
CCCCCGCCTC CGCCTCAGAG AAACACCTCC TACCTCAAAA CCCAGGTCGC CTCCCCAGAC 
ACCATTTACA CTGCCAAGTT TGTCTCGTAC AATGACGAGG AGGAGGAGGA GGATGAGGAA 
GATGCGGGCC TAACAGGTCA GGTTAAGCTC TCGGCCACCC GCAAGTCCTA CGGCGACCTT 
CCGCCTGCCC CAAAGCCCCA GCCGCCGCCA ACCGCGCGCA AGCCCCGTCC CGTCTCAGAT 
GGCATCTTTC TGTCCAACTC ATTCCAGCTG CCCGCTGGGC TCAATGCCAA CAGTACTGCT 
CTCAAGACAG GGCAGCCCCC TCTCCCCTTC ACCAAACCCA GCTTCATCCC ATCAGCCAAC 
TCCAAAGGAT TTAATTCATA CACTGGAAAC ACAACAGGAC TAGTATCAGG AGAGGTGTAT 
AAAGACCCGC GAGACAAGTG GACCAAAGGC CCGGATCAAG AGAACACAAT AGGAGGAGCT 
CCAGAAAACC TGACCTTCAA GGAGCGCCAG AGACTCTTCT CGCAGGGGAA AGAGGTCTCC 
AATAAAGTCA AAGCGTCCCG AAAGCTGATG GAGCTGGAGA ACGAACTCAA CATAAAACAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	mllt4a
Entrez Gene ID	558359
Full Name	afadin, adherens junction formation factor a
Synonyms	mllt4,mllt4a,si:ch211-278j12.1
General protein information	Preferred Names afadin, adherens junction formation factor a Names afadin MLLT4, super elongation complex subunit a myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4a myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Caenorhabditis elegans (roundworm)	afd-1	NP_001021660.1
Gallus gallus (chicken)	MLLT4	XP_004935593.1
Danio rerio (zebrafish)	mllt4	XP_686655.5
Homo sapiens (human)	MLLT4	NP_001193937.1
Pan troglodytes (chimpanzee)	MLLT4	XP_518857.4
	MLLT4	XP_001083153.1
Canis lupus familiaris (dog)	MLLT4	XP_005615632.1
Bos taurus (cattle)	MLLT4	XP_005211198.1
Mus musculus (house mouse)	Mllt4	NP_034936.1
Rattus norvegicus (Norway rat)	Mllt4	NP_037349.1
Xenopus tropicalis (tropical clawed frog)	mllt4	XP_002931548.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

mllt4a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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