ORF » Species Summary » Caenorhabditis elegans » afd-1 cDNA ORF clone

afd-1 cDNA ORF clone, Caenorhabditis elegans

  • Gene

  • Clones

  • gRNAs

The following afd-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the afd-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa336876 NM_001026488.3
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCa336162 NM_001306401.2
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCa336163 NM_001306402.2
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCa336164 NM_001306403.2
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCa336165 NM_001306404.2
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCa336166 NM_001306405.2
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCa336167 NM_001306406.2
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCa336168 NM_001306407.2
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCa336169 NM_001306408.2
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCa337252 NM_001306529.2
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCa529735 NM_001356800.2
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCa335223 NM_001026489.5
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCa335224 NM_001026490.5
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCa336161 NM_001306400.2
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCa336875 NM_058537.5
Latest version!		 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
View Old Accession Versions >>
OCa336169 NM_001306408.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa529735 NM_001356800.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCa336162 NM_001306401.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $797.30
$1139.00
OCa336163 NM_001306402.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa336168 NM_001306407.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $629.30
$899.00
OCa335223 NM_001026489.4 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa337252 NM_001306529.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa336875 NM_058537.4 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $881.30
$1259.00
OCa336161 NM_001306400.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa336165 NM_001306404.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa336167 NM_001306406.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa336876 NM_001026488.2 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCa336166 NM_001306405.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OCa335224 NM_001026490.4 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa336164 NM_001306403.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    afd-1 ( NM_001026488.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021659.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336876
    Clone ID Related Accession (Same CDS sequence) NM_001026488.2 , NM_001026488.3
    Accession Version NM_001026488.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001026488.1. COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGTTCTTA TTGCAGTTTA TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001021659.1
    CDS1..2037
    Translation

    Target ORF information:

    RefSeq Version NM_001026488.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001026488.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGTTCTTA TTGCAGTTTA TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001026488.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021659.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.
    Price & Availability↑
    CloneID OCa336876
    Clone ID Related Accession (Same CDS sequence) NM_001026488.2 , NM_001026488.3
    Accession Version NM_001026488.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001026488.2. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGTTCTTA TTGCAGTTTA TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001021659.1
    CDS10..2046
    Translation

    Target ORF information:

    RefSeq Version NM_001026488.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001026488.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGTTCTTA TTGCAGTTTA TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306401.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293330.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336162
    Clone ID Related Accession (Same CDS sequence) NM_001306401.1 , NM_001306401.2
    Accession Version NM_001306401.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCAGACC TCCGTGAAAT CGACGGACGG CCCATTCCAC CAACAGAGTG CCCGCTTTTC 
    GAGATGACTG CTCGAAGTGG AAACGGCGAG AATGGATTCG ATTCGTTTTT GGCGATCAAG 
    CGGAAACCGC ATGATTATCA AGTCCCCTCC ACCTCAGTTG TTGCTCCATC ATCAGTCACC 
    CTACCAATGT CTCCATTAAC ATCACTTCCA CCACAAATTC ACAATCTTCC AGTGCTTATT 
    GTGATGAATA ATGATGGTCA CTTGTCAACA CACCGAATAA CGTTACACGA GGGAGTAACA 
    GAGGTTGGAA GTGACAGTCA AATGTCCAAT TTCTCACAGC ATAATATTTA TCTCGATGGT 
    CCGGATATTC GTGGACGCCA CGCGGCAATC GCTTTCATGG AAGGCGTCGT CACACTAACA 
    CCATCCACTC GTGATGCCTA CCTAGAGGTG AACGGCCATC AACTGATGCA GACGGAGATC 
    CTCCGGGACG GTGATCACAT TCGCATCGGA GCCGATCATT CGTTCAAGTT TAGCACGTCG 
    TCGTCACAAG CAAATCAGAT TAACAGTACT AATATCAGCA ACTCGAACCA ACGACACTTC 
    CAACAACAGA CATTTTATCC GGAAATGGCT GATTCACGAG CTTCAACTGC CAGGTGTGCA 
    CCATCGGATT ACTGTAGTCT ACCACAACAG AACGATTATT ATCCGCCGAG CATGTCCGGC 
    TCATCAGTCT CATCATCACG GCAGGAGGAT CTTATGATGC ATTCCAATAA TAACATCCTG 
    CCACCGTACT CTACAAAATC CGCGCAGGAA CCCCTCCAAC TGCCGCTCTC AATTCAAATC 
    GGCGACTACA ATGTATGGGA TTTCCTGGCC GAAGTGATGT CGAATCGTGC GGCAACCTCA 
    TCGGAAATGT CCACATTCCG CCTATCGCCT GCCTACTCTC TCTACCTGGC ACTTCGATCA 
    TTCCACACAC ACAATAAACC GTACTCTGTG CAATTCTTCT CACGGATCGA GTCGAATTTC 
    AAGCAAATCG CTCAGGAATG CTCGTCGAAG GATGATCTGA TCTTTTGGCT TGCCAACGCA 
    TCGGAATTCA ATCATCTTGT CGAGCGGGAC ACTGAGCTGA AAGCGTCGAG ATATGGCAAA 
    ATATCTGTGG AAATTGAGAG AATATTCCGG CGATTGGTTG CAGTGCTTCA AAGTGTCATC 
    CGTCCGGCGG CGCGGGCTCT GTTGGACATT CATCTGAATG ATCACGACGC GACCGGCGAT 
    GTGATTCATC TTCTCGACAC CACGATGCGC CAGGCGAGAA TTTGTGGTCT CAACGCGGCG 
    CTCACAATTC AACTGTGCGG GCATCTCCTC CACATGGTCA ATGCCTTTGT GTTCAACAGC 
    TTGGTCTCAG TCAACCTTCC AGCTGCCCAG TTGAATACTC GCCTCGGAAA GTGTCTTCAG 
    TATCGGATAG AGTCGATTCA CAGATTCTGC GAACGAATGG GCGTCGAGAT GGCCGCCGAG 
    TGCCATTTGG ATAGATCCCG TCAGGCCGCT AACCTTCTCG CCACGCAGAA GAATGATGTT 
    GCCGCGTTGG GATCGACGTG CTATAAGCTG AACAGCCTCC AAGTGGTTCA TATCCTGTCG 
    GGATTCGAAC CAGACGACGG CGAGATTCCA TGTGACGATG ACGTCGTTGA TCGGATCTGT 
    AGACTCGCTG AAAAACAGGC GGATAAGCTG ACGTTGGAGG ATGGGCAGCG CGTGACACTG 
    GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC CTGATCCCTC AGGATGGTTA CTTTGTCGAG 
    CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG CAGTACTTGC AGCAACTTCA GCATAGGCGC 
    CTCTGTCATA CGGTGTCGCT TACACCGGCA CCGCCGGCTG CGATGACCCA TTCGATGCAT 
    GCGATGCATA TTGGAGCAAG CGATGCTCAT TATCATCAGC ATCAGCATCA GCAGCAGCAG 
    AACCCGATTG TGAGAAGTGT GTCGCAGACT ACACTGACCA GTATTCCGAC TACACAATTT 
    GGCGACAGTT TTGGAAGTGC CGTGTCGGAT ACGATATCGA AGGTGACACT GCGGAAGAAT 
    GGAGGAGGCA TCGGATTGAG TATTGTCGCT GCTCAGGGTG TTGGTGATCG TCAAATGGGA 
    ATTTATGTGA AGAAGGTTGT CGAAGGAACA CCGGCTGCAC ACGATGGACG ACTCGAGACT 
    GGTGATCAGT TATTGAGTGT TAACGGACAT TCGTTAATTG GAATTTCACA GGAAGACGCC 
    GCCCGTCTCA TGACCCAAAG TGGAAACGAG GTGCATTTCG ACGTCCGGAA AAGAGCCGCT 
    CACCGTAACG GGCTCAGCAA TTGGCTCGGA TCCCAAACAC CGACAACTTC AAATTTCATT 
    GCACCATCTC CATCTACAGT ACCCCCGCCG AGTTATTCAC ATGTTGGACG GACTCCGTCT 
    TATGATGGAC AACCACCACC GAGTTATAAT CACAGAAATG GTGGAGCCAG CGGAGCAGGA 
    GGATCATCTG GAAGAAATAG TGCTTTCGTA GCACCACTTT CGAATTCATC ATCAAATGCG 
    TCGAGTAGTC ATATGCAACA ACACTATGCT CATCATAATA TGATGCATAA TGGCAATTTG 
    AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT CCGAAACATT ATCGAAGCTC GTCGGCTAGT 
    GATTTGCAGC ACGATCCAAA CGCCTCATTC TCTCAATCAT CAATGACTGG AAGTGCGAGA 
    AGTTCAGTGT TTGACAAGCT TCCATCTCAC TACCGTCACT CGACACGGCC TACAGTAATC 
    CAGCCAACAC GTCCAAGTGC TCAATCTCCA TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG 
    CCGGTCAGCA ATCTGTACCG TCCGACGTCG GCGTCGAATC TGTTTGCACC GCCCAGAAAT 
    TCGGGCGGTC TCGTCGGATA TTCGAGTAAC GCGGCACGGG AGTCGACACA GGATATTCAT 
    CGAGCGCTAA ATGACCTGGA ACTCAACTCA CCGCTCGCCA GATCCACGCC GAATGAGGAG 
    TACCGTATCC CGCCGCCTAG AGTACCTACA GTAACCTCCA CCTCCTCCAA CTATGCCCCT 
    CTTCATTCTA TTCGACCGGC AAGCATGTAT GGATATCAGA GCCCGAGCTC TTCTACTACA 
    GTAATCCCGG CTCCAAAAGG AGTGGCTTCT TCCTCTAATA ACACTTCTCC CCGAGTGAAT 
    GTGACGTTGG GTGGAGCATT GAGCTCATCG AGATCTCATA ATAGTCTTTC ACAGGAGCCA 
    GCCATTGTTA TCAGGCCTGT CGACCTGAAT ATGCCGAAAG CGACACCACC GGCGTCGATT 
    CGTGGAGCTG TGCCGACGGC GATTACACAG GCTCTTCAAC TTCAACCAAA CGGCGGTGGA 
    GCAGTACTTC GTCAGGTTCG GCCAGCATCC GGGCTCCGCG AGCGACTTCA GGACGCTGAT 
    GTTCGGAATT TGGAGAAAAC GAACACAGCA CTGATGAGCT ATGAAGCAGT GAATGAGGAG 
    CTCGATCGAC TCGACGCTAA GCAGAATACG ATGACAATTG ACGAGCAACG GAGATATCGA 
    GAGCTACTGA ACGTTGTCGC CGAGCAGAGC CGAGCAAGGA AGCTGCTCTC GGAGATCAGA 
    TCACCACCTA CATCTTATCA AGAACGTGAA ATTCCGATTC ATGTGCAAAA AGAGCAGCAG 
    CAGCACCGGC GGGAAACTGT GATTGATGAT ATTCCACACT CGAGCTCAAG TGTTGAGAAT 
    GTGTCACCTG TGAGCCCGCC GGAAAGTCGA AAAGTTCAAT TTATTGATGA AGTTGTGCAG 
    TATCAGACGC CGACCAGCGG AGCCAATCAC GCTTTCTTTG ATGATGTGCC GGTTTTGGAT 
    TCGCCTGGGA TCGTCGGCAC CAACGAAATC TACCGTGATC CCCGCCAACG TCGGCTCAAC 
    GAGCTCCAAG ACCGCAATCG CAGCTCGGAA AATGGAGCGG CGGACGGCGC GAAGCTCGGC 
    TTCCGCGACA AGCAGCGCCT ATTCGCACGG CAAATCGGCG AGGACACGGC ACCACGCCAA 
    CGAATGAATG AGAGCAGTGC TCAGCGGCTC ATTGAGACGG ACATTGCCGC CACCCCGCAA 
    AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293330.1
    CDS1..4149
    Translation

    Target ORF information:

    RefSeq Version NM_001306401.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306401.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCAGACC TCCGTGAAAT CGACGGACGG CCCATTCCAC CAACAGAGTG CCCGCTTTTC 
    GAGATGACTG CTCGAAGTGG AAACGGCGAG AATGGATTCG ATTCGTTTTT GGCGATCAAG 
    CGGAAACCGC ATGATTATCA AGTCCCCTCC ACCTCAGTTG TTGCTCCATC ATCAGTCACC 
    CTACCAATGT CTCCATTAAC ATCACTTCCA CCACAAATTC ACAATCTTCC AGTGCTTATT 
    GTGATGAATA ATGATGGTCA CTTGTCAACA CACCGAATAA CGTTACACGA GGGAGTAACA 
    GAGGTTGGAA GTGACAGTCA AATGTCCAAT TTCTCACAGC ATAATATTTA TCTCGATGGT 
    CCGGATATTC GTGGACGCCA CGCGGCAATC GCTTTCATGG AAGGCGTCGT CACACTAACA 
    CCATCCACTC GTGATGCCTA CCTAGAGGTG AACGGCCATC AACTGATGCA GACGGAGATC 
    CTCCGGGACG GTGATCACAT TCGCATCGGA GCCGATCATT CGTTCAAGTT TAGCACGTCG 
    TCGTCACAAG CAAATCAGAT TAACAGTACT AATATCAGCA ACTCGAACCA ACGACACTTC 
    CAACAACAGA CATTTTATCC GGAAATGGCT GATTCACGAG CTTCAACTGC CAGGTGTGCA 
    CCATCGGATT ACTGTAGTCT ACCACAACAG AACGATTATT ATCCGCCGAG CATGTCCGGC 
    TCATCAGTCT CATCATCACG GCAGGAGGAT CTTATGATGC ATTCCAATAA TAACATCCTG 
    CCACCGTACT CTACAAAATC CGCGCAGGAA CCCCTCCAAC TGCCGCTCTC AATTCAAATC 
    GGCGACTACA ATGTATGGGA TTTCCTGGCC GAAGTGATGT CGAATCGTGC GGCAACCTCA 
    TCGGAAATGT CCACATTCCG CCTATCGCCT GCCTACTCTC TCTACCTGGC ACTTCGATCA 
    TTCCACACAC ACAATAAACC GTACTCTGTG CAATTCTTCT CACGGATCGA GTCGAATTTC 
    AAGCAAATCG CTCAGGAATG CTCGTCGAAG GATGATCTGA TCTTTTGGCT TGCCAACGCA 
    TCGGAATTCA ATCATCTTGT CGAGCGGGAC ACTGAGCTGA AAGCGTCGAG ATATGGCAAA 
    ATATCTGTGG AAATTGAGAG AATATTCCGG CGATTGGTTG CAGTGCTTCA AAGTGTCATC 
    CGTCCGGCGG CGCGGGCTCT GTTGGACATT CATCTGAATG ATCACGACGC GACCGGCGAT 
    GTGATTCATC TTCTCGACAC CACGATGCGC CAGGCGAGAA TTTGTGGTCT CAACGCGGCG 
    CTCACAATTC AACTGTGCGG GCATCTCCTC CACATGGTCA ATGCCTTTGT GTTCAACAGC 
    TTGGTCTCAG TCAACCTTCC AGCTGCCCAG TTGAATACTC GCCTCGGAAA GTGTCTTCAG 
    TATCGGATAG AGTCGATTCA CAGATTCTGC GAACGAATGG GCGTCGAGAT GGCCGCCGAG 
    TGCCATTTGG ATAGATCCCG TCAGGCCGCT AACCTTCTCG CCACGCAGAA GAATGATGTT 
    GCCGCGTTGG GATCGACGTG CTATAAGCTG AACAGCCTCC AAGTGGTTCA TATCCTGTCG 
    GGATTCGAAC CAGACGACGG CGAGATTCCA TGTGACGATG ACGTCGTTGA TCGGATCTGT 
    AGACTCGCTG AAAAACAGGC GGATAAGCTG ACGTTGGAGG ATGGGCAGCG CGTGACACTG 
    GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC CTGATCCCTC AGGATGGTTA CTTTGTCGAG 
    CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG CAGTACTTGC AGCAACTTCA GCATAGGCGC 
    CTCTGTCATA CGGTGTCGCT TACACCGGCA CCGCCGGCTG CGATGACCCA TTCGATGCAT 
    GCGATGCATA TTGGAGCAAG CGATGCTCAT TATCATCAGC ATCAGCATCA GCAGCAGCAG 
    AACCCGATTG TGAGAAGTGT GTCGCAGACT ACACTGACCA GTATTCCGAC TACACAATTT 
    GGCGACAGTT TTGGAAGTGC CGTGTCGGAT ACGATATCGA AGGTGACACT GCGGAAGAAT 
    GGAGGAGGCA TCGGATTGAG TATTGTCGCT GCTCAGGGTG TTGGTGATCG TCAAATGGGA 
    ATTTATGTGA AGAAGGTTGT CGAAGGAACA CCGGCTGCAC ACGATGGACG ACTCGAGACT 
    GGTGATCAGT TATTGAGTGT TAACGGACAT TCGTTAATTG GAATTTCACA GGAAGACGCC 
    GCCCGTCTCA TGACCCAAAG TGGAAACGAG GTGCATTTCG ACGTCCGGAA AAGAGCCGCT 
    CACCGTAACG GGCTCAGCAA TTGGCTCGGA TCCCAAACAC CGACAACTTC AAATTTCATT 
    GCACCATCTC CATCTACAGT ACCCCCGCCG AGTTATTCAC ATGTTGGACG GACTCCGTCT 
    TATGATGGAC AACCACCACC GAGTTATAAT CACAGAAATG GTGGAGCCAG CGGAGCAGGA 
    GGATCATCTG GAAGAAATAG TGCTTTCGTA GCACCACTTT CGAATTCATC ATCAAATGCG 
    TCGAGTAGTC ATATGCAACA ACACTATGCT CATCATAATA TGATGCATAA TGGCAATTTG 
    AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT CCGAAACATT ATCGAAGCTC GTCGGCTAGT 
    GATTTGCAGC ACGATCCAAA CGCCTCATTC TCTCAATCAT CAATGACTGG AAGTGCGAGA 
    AGTTCAGTGT TTGACAAGCT TCCATCTCAC TACCGTCACT CGACACGGCC TACAGTAATC 
    CAGCCAACAC GTCCAAGTGC TCAATCTCCA TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG 
    CCGGTCAGCA ATCTGTACCG TCCGACGTCG GCGTCGAATC TGTTTGCACC GCCCAGAAAT 
    TCGGGCGGTC TCGTCGGATA TTCGAGTAAC GCGGCACGGG AGTCGACACA GGATATTCAT 
    CGAGCGCTAA ATGACCTGGA ACTCAACTCA CCGCTCGCCA GATCCACGCC GAATGAGGAG 
    TACCGTATCC CGCCGCCTAG AGTACCTACA GTAACCTCCA CCTCCTCCAA CTATGCCCCT 
    CTTCATTCTA TTCGACCGGC AAGCATGTAT GGATATCAGA GCCCGAGCTC TTCTACTACA 
    GTAATCCCGG CTCCAAAAGG AGTGGCTTCT TCCTCTAATA ACACTTCTCC CCGAGTGAAT 
    GTGACGTTGG GTGGAGCATT GAGCTCATCG AGATCTCATA ATAGTCTTTC ACAGGAGCCA 
    GCCATTGTTA TCAGGCCTGT CGACCTGAAT ATGCCGAAAG CGACACCACC GGCGTCGATT 
    CGTGGAGCTG TGCCGACGGC GATTACACAG GCTCTTCAAC TTCAACCAAA CGGCGGTGGA 
    GCAGTACTTC GTCAGGTTCG GCCAGCATCC GGGCTCCGCG AGCGACTTCA GGACGCTGAT 
    GTTCGGAATT TGGAGAAAAC GAACACAGCA CTGATGAGCT ATGAAGCAGT GAATGAGGAG 
    CTCGATCGAC TCGACGCTAA GCAGAATACG ATGACAATTG ACGAGCAACG GAGATATCGA 
    GAGCTACTGA ACGTTGTCGC CGAGCAGAGC CGAGCAAGGA AGCTGCTCTC GGAGATCAGA 
    TCACCACCTA CATCTTATCA AGAACGTGAA ATTCCGATTC ATGTGCAAAA AGAGCAGCAG 
    CAGCACCGGC GGGAAACTGT GATTGATGAT ATTCCACACT CGAGCTCAAG TGTTGAGAAT 
    GTGTCACCTG TGAGCCCGCC GGAAAGTCGA AAAGTTCAAT TTATTGATGA AGTTGTGCAG 
    TATCAGACGC CGACCAGCGG AGCCAATCAC GCTTTCTTTG ATGATGTGCC GGTTTTGGAT 
    TCGCCTGGGA TCGTCGGCAC CAACGAAATC TACCGTGATC CCCGCCAACG TCGGCTCAAC 
    GAGCTCCAAG ACCGCAATCG CAGCTCGGAA AATGGAGCGG CGGACGGCGC GAAGCTCGGC 
    TTCCGCGACA AGCAGCGCCT ATTCGCACGG CAAATCGGCG AGGACACGGC ACCACGCCAA 
    CGAATGAATG AGAGCAGTGC TCAGCGGCTC ATTGAGACGG ACATTGCCGC CACCCCGCAA 
    AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306403.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293332.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336164
    Clone ID Related Accession (Same CDS sequence) NM_001306403.1 , NM_001306403.2
    Accession Version NM_001306403.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    CTAAATGACC TGGAACTCAA CTCACCGCTC GCCAGATCCA CGCCGAATGA GGAGTACCGT 
    ATCCCGCCGC CTAGAGTACC TACAGTAACC TCCACCTCCT CCAACTATGC CCCTCTTCAT 
    TCTATTCGAC CGGCAAGCAT GTATGGATAT CAGAGCCCGA GCTCTTCTAC TACAGTAATC 
    CCGGCTCCAA AAGGAGTGGC TTCTTCCTCT AATAACACTT CTCCCCGAGT GAATGTGACG 
    TTGGGTGGAG CATTGAGCTC ATCGAGATCT CATAATAGTC TTTCACAGGA GCCAGCCATT 
    GTTATCAGGC CTGTCGACCT GAATATGCCG AAAGCGACAC CACCGGCGTC GATTCGTGGA 
    GCTGTGCCGA CGGCGATTAC ACAGGCTCTT CAACTTCAAC CAAACGGCGG TGGAGCAGTA 
    CTTCGTCAGG TTCGGCCAGC ATCCGGGCTC CGCGAGCGAC TTCAGGACGC TGATGTTCGG 
    AATTTGGAGA AAACGAACAC AGCACTGATG AGCTATGAAG CAGTGAATGA GGAGCTCGAT 
    CGACTCGACG CTAAGCAGAA TACGATGACA ATTGACGAGC AACGGAGATA TCGAGAGCTA 
    CTGAACGTTG TCGCCGAGCA GAGCCGAGCA AGGAAGCTGC TCTCGGAGAT CAGATCACCA 
    CCTACATCTT ATCAAGAACG TGAAATTCCG ATTCATGTGC AAAAAGAGCA GCAGCAGCAC 
    CGGCGGGAAA CTGTGATTGA TGATATTCCA CACTCGAGCT CAAGTGTTGA GAATGTGTCA 
    CCTGTGAGCC CGCCGGAAAG TCGAAAAGTT CAATTTATTG ATGAAGTTGT GCAGTATCAG 
    ACGCCGACCA GCGGAGCCAA TCACGCTTTC TTTGATGATG TGCCGGTTTT GGATTCGCCT 
    GGGATCGTCG GCACCAACGA AATCTACCGT GATCCCCGCC AACGTCGGCT CAACGAGCTC 
    CAAGACCGCA ATCGCAGCTC GGAAAATGGA GCGGCGGACG GCGCGAAGCT CGGCTTCCGC 
    GACAAGCAGC GCCTATTCGC ACGGCAAATC GGCGAGGACA CGGCACCACG CCAACGAATG 
    AATGAGAGCA GTGCTCAGCG GCTCATTGAG ACGGACATTG CCGCCACCCC GCAAAAGCCA 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293332.1
    CDS1..3963
    Translation

    Target ORF information:

    RefSeq Version NM_001306403.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306403.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    CTAAATGACC TGGAACTCAA CTCACCGCTC GCCAGATCCA CGCCGAATGA GGAGTACCGT 
    ATCCCGCCGC CTAGAGTACC TACAGTAACC TCCACCTCCT CCAACTATGC CCCTCTTCAT 
    TCTATTCGAC CGGCAAGCAT GTATGGATAT CAGAGCCCGA GCTCTTCTAC TACAGTAATC 
    CCGGCTCCAA AAGGAGTGGC TTCTTCCTCT AATAACACTT CTCCCCGAGT GAATGTGACG 
    TTGGGTGGAG CATTGAGCTC ATCGAGATCT CATAATAGTC TTTCACAGGA GCCAGCCATT 
    GTTATCAGGC CTGTCGACCT GAATATGCCG AAAGCGACAC CACCGGCGTC GATTCGTGGA 
    GCTGTGCCGA CGGCGATTAC ACAGGCTCTT CAACTTCAAC CAAACGGCGG TGGAGCAGTA 
    CTTCGTCAGG TTCGGCCAGC ATCCGGGCTC CGCGAGCGAC TTCAGGACGC TGATGTTCGG 
    AATTTGGAGA AAACGAACAC AGCACTGATG AGCTATGAAG CAGTGAATGA GGAGCTCGAT 
    CGACTCGACG CTAAGCAGAA TACGATGACA ATTGACGAGC AACGGAGATA TCGAGAGCTA 
    CTGAACGTTG TCGCCGAGCA GAGCCGAGCA AGGAAGCTGC TCTCGGAGAT CAGATCACCA 
    CCTACATCTT ATCAAGAACG TGAAATTCCG ATTCATGTGC AAAAAGAGCA GCAGCAGCAC 
    CGGCGGGAAA CTGTGATTGA TGATATTCCA CACTCGAGCT CAAGTGTTGA GAATGTGTCA 
    CCTGTGAGCC CGCCGGAAAG TCGAAAAGTT CAATTTATTG ATGAAGTTGT GCAGTATCAG 
    ACGCCGACCA GCGGAGCCAA TCACGCTTTC TTTGATGATG TGCCGGTTTT GGATTCGCCT 
    GGGATCGTCG GCACCAACGA AATCTACCGT GATCCCCGCC AACGTCGGCT CAACGAGCTC 
    CAAGACCGCA ATCGCAGCTC GGAAAATGGA GCGGCGGACG GCGCGAAGCT CGGCTTCCGC 
    GACAAGCAGC GCCTATTCGC ACGGCAAATC GGCGAGGACA CGGCACCACG CCAACGAATG 
    AATGAGAGCA GTGCTCAGCG GCTCATTGAG ACGGACATTG CCGCCACCCC GCAAAAGCCA 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306405.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293334.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336166
    Clone ID Related Accession (Same CDS sequence) NM_001306405.2 , NM_001306405.1
    Accession Version NM_001306405.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3684bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGACGG AGATCCTCCG GGACGGTGAT CACATTCGCA TCGGAGCCGA TCATTCGTTC 
    AAGTTTAGCA CGTCGTCGTC ACAAGCAAAT CAGATTAACA GTACTAATAT CAGCAACTCG 
    AACCAACGAC ACTTCCAACA ACAGACATTT TATCCGGAAA TGGCTGATTC ACGAGCTTCA 
    ACTGCCAGGT GTGCACCATC GGATTACTGT AGTCTACCAC AACAGAACGA TTATTATCCG 
    CCGAGCATGT CCGGCTCATC AGTCTCATCA TCACGGCAGG AGGATCTTAT GATGCATTCC 
    AATAATAACA TCCTGCCACC GTACTCTACA AAATCCGCGC AGGAACCCCT CCAACTGCCG 
    CTCTCAATTC AAATCGGCGA CTACAATGTA TGGGATTTCC TGGCCGAAGT GATGTCGAAT 
    CGTGCGGCAA CCTCATCGGA AATGTCCACA TTCCGCCTAT CGCCTGCCTA CTCTCTCTAC 
    CTGGCACTTC GATCATTCCA CACACACAAT AAACCGTACT CTGTGCAATT CTTCTCACGG 
    ATCGAGTCGA ATTTCAAGCA AATCGCTCAG GAATGCTCGT CGAAGGATGA TCTGATCTTT 
    TGGCTTGCCA ACGCATCGGA ATTCAATCAT CTTGTCGAGC GGGACACTGA GCTGAAAGCG 
    TCGAGATATG GCAAAATATC TGTGGAAATT GAGAGAATAT TCCGGCGATT GGTTGCAGTG 
    CTTCAAAGTG TCATCCGTCC GGCGGCGCGG GCTCTGTTGG ACATTCATCT GAATGATCAC 
    GACGCGACCG GCGATGTGAT TCATCTTCTC GACACCACGA TGCGCCAGGC GAGAATTTGT 
    GGTCTCAACG CGGCGCTCAC AATTCAACTG TGCGGGCATC TCCTCCACAT GGTCAATGCC 
    TTTGTGTTCA ACAGCTTGGT CTCAGTCAAC CTTCCAGCTG CCCAGTTGAA TACTCGCCTC 
    GGAAAGTGTC TTCAGTATCG GATAGAGTCG ATTCACAGAT TCTGCGAACG AATGGGCGTC 
    GAGATGGCCG CCGAGTGCCA TTTGGATAGA TCCCGTCAGG CCGCTAACCT TCTCGCCACG 
    CAGAAGAATG ATGTTGCCGC GTTGGGATCG ACGTGCTATA AGCTGAACAG CCTCCAAGTG 
    GTTCATATCC TGTCGGGATT CGAACCAGAC GACGGCGAGA TTCCATGTGA CGATGACGTC 
    GTTGATCGGA TCTGTAGACT CGCTGAAAAA CAGGCGGATA AGCTGACGTT GGAGGATGGG 
    CAGCGCGTGA CACTGGAGGA GTCCGACGAG CTGAACCTTC CGTTCCTGAT CCCTCAGGAT 
    GGTTACTTTG TCGAGCAGTT CCGAACGGTG CCAGATGGTC TTTGGCAGTA CTTGCAGCAA 
    CTTCAGCATA GGCGCCTCTG TCATACGGTG TCGCTTACAC CGGCACCGCC GGCTGCGATG 
    ACCCATTCGA TGCATGCGAT GCATATTGGA GCAAGCGATG CTCATTATCA TCAGCATCAG 
    CATCAGCAGC AGCAGAACCC GATTGTGAGA AGTGTGTCGC AGACTACACT GACCAGTATT 
    CCGACTACAC AATTTGGCGA CAGTTTTGGA AGTGCCGTGT CGGATACGAT ATCGAAGGTG 
    ACACTGCGGA AGAATGGAGG AGGCATCGGA TTGAGTATTG TCGCTGCTCA GGGTGTTGGT 
    GATCGTCAAA TGGGAATTTA TGTGAAGAAG GTTGTCGAAG GAACACCGGC TGCACACGAT 
    GGACGACTCG AGACTGGTGA TCAGTTATTG AGTGTTAACG GACATTCGTT AATTGGAATT 
    TCACAGGAAG ACGCCGCCCG TCTCATGACC CAAAGTGGAA ACGAGGTGCA TTTCGACGTC 
    CGGAAAAGAG CCGCTCACCG TAACGGGCTC AGCAATTGGC TCGGATCCCA AACACCGACA 
    ACTTCAAATT TCATTGCACC ATCTCCATCT ACAGTACCCC CGCCGAGTTA TTCACATGTT 
    GGACGGACTC CGTCTTATGA TGGACAACCA CCACCGAGTT ATAATCACAG AAATGGTGGA 
    GCCAGCGGAG CAGGAGGATC ATCTGGAAGA AATAGTGCTT TCGTAGCACC ACTTTCGAAT 
    TCATCATCAA ATGCGTCGAG TAGTCATATG CAACAACACT ATGCTCATCA TAATATGATG 
    CATAATGGCA ATTTGAGTGC TGCTCCGGGA GCTCCCGCGG GGGATCCGAA ACATTATCGA 
    AGCTCGTCGG CTAGTGATTT GCAGCACGAT CCAAACGCCT CATTCTCTCA ATCATCAATG 
    ACTGGAAGTG CGAGAAGTTC AGTGTTTGAC AAGCTTCCAT CTCACTACCG TCACTCGACA 
    CGGCCTACAG TAATCCAGCC AACACGTCCA AGTGCTCAAT CTCCATCGGC TCTTCGAAAA 
    GCGGCCGCCG CCTCGCCGGT CAGCAATCTG TACCGTCCGA CGTCGGCGTC GAATCTGTTT 
    GCACCGCCCA GAAATTCGGG CGGTCTCGTC GGATATTCGA GTAACGCGGC ACGGGAGTCG 
    ACACAGGATA TTCATCGAGC GCTAAATGAC CTGGAACTCA ACTCACCGCT CGCCAGATCC 
    ACGCCGAATG AGGAGTACCG TATCCCGCCG CCTAGAGTAC CTACAGTAAC CTCCACCTCC 
    TCCAACTATG CCCCTCTTCA TTCTATTCGA CCGGCAAGCA TGTATGGATA TCAGAGCCCG 
    AGCTCTTCTA CTACAGTAAT CCCGGCTCCA AAAGGAGTGG CTTCTTCCTC TAATAACACT 
    TCTCCCCGAG TGAATGTGAC GTTGGGTGGA GCATTGAGCT CATCGAGATC TCATAATAGT 
    CTTTCACAGG AGCCAGCCAT TGTTATCAGG CCTGTCGACC TGAATATGCC GAAAGCGACA 
    CCACCGGCGT CGATTCGTGG AGCTGTGCCG ACGGCGATTA CACAGGCTCT TCAACTTCAA 
    CCAAACGGCG GTGGAGCAGT ACTTCGTCAG GTTCGGCCAG CATCCGGGCT CCGCGAGCGA 
    CTTCAGGACG CTGATGTTCG GAATTTGGAG AAAACGAACA CAGCACTGAT GAGCTATGAA 
    GCAGTGAATG AGGAGCTCGA TCGACTCGAC GCTAAGCAGA ATACGATGAC AATTGACGAG 
    CAACGGAGAT ATCGAGAGCT ACTGAACGTT GTCGCCGAGC AGAGCCGAGC AAGGAAGCTG 
    CTCTCGGAGA TCAGATCACC ACCTACATCT TATCAAGAAC GTGAAATTCC GATTCATGTG 
    CAAAAAGAGC AGCAGCAGCA CCGGCGGGAA ACTGTGATTG ATGATATTCC ACACTCGAGC 
    TCAAGTGTTG AGAATGTGTC ACCTGTGAGC CCGCCGGAAA GTCGAAAAGT TCAATTTATT 
    GATGAAGTTG TGCAGTATCA GACGCCGACC AGCGGAGCCA ATCACGCTTT CTTTGATGAT 
    GTGCCGGTTT TGGATTCGCC TGGGATCGTC GGCACCAACG AAATCTACCG TGATCCCCGC 
    CAACGTCGGC TCAACGAGCT CCAAGACCGC AATCGCAGCT CGGAAAATGG AGCGGCGGAC 
    GGCGCGAAGC TCGGCTTCCG CGACAAGCAG CGCCTATTCG CACGGCAAAT CGGCGAGGAC 
    ACGGCACCAC GCCAACGAAT GAATGAGAGC AGTGCTCAGC GGCTCATTGA GACGGACATT 
    GCCGCCACCC CGCAAAAGCC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293334.1
    CDS1..3684
    Translation

    Target ORF information:

    RefSeq Version NM_001306405.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306405.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGACGG AGATCCTCCG GGACGGTGAT CACATTCGCA TCGGAGCCGA TCATTCGTTC 
    AAGTTTAGCA CGTCGTCGTC ACAAGCAAAT CAGATTAACA GTACTAATAT CAGCAACTCG 
    AACCAACGAC ACTTCCAACA ACAGACATTT TATCCGGAAA TGGCTGATTC ACGAGCTTCA 
    ACTGCCAGGT GTGCACCATC GGATTACTGT AGTCTACCAC AACAGAACGA TTATTATCCG 
    CCGAGCATGT CCGGCTCATC AGTCTCATCA TCACGGCAGG AGGATCTTAT GATGCATTCC 
    AATAATAACA TCCTGCCACC GTACTCTACA AAATCCGCGC AGGAACCCCT CCAACTGCCG 
    CTCTCAATTC AAATCGGCGA CTACAATGTA TGGGATTTCC TGGCCGAAGT GATGTCGAAT 
    CGTGCGGCAA CCTCATCGGA AATGTCCACA TTCCGCCTAT CGCCTGCCTA CTCTCTCTAC 
    CTGGCACTTC GATCATTCCA CACACACAAT AAACCGTACT CTGTGCAATT CTTCTCACGG 
    ATCGAGTCGA ATTTCAAGCA AATCGCTCAG GAATGCTCGT CGAAGGATGA TCTGATCTTT 
    TGGCTTGCCA ACGCATCGGA ATTCAATCAT CTTGTCGAGC GGGACACTGA GCTGAAAGCG 
    TCGAGATATG GCAAAATATC TGTGGAAATT GAGAGAATAT TCCGGCGATT GGTTGCAGTG 
    CTTCAAAGTG TCATCCGTCC GGCGGCGCGG GCTCTGTTGG ACATTCATCT GAATGATCAC 
    GACGCGACCG GCGATGTGAT TCATCTTCTC GACACCACGA TGCGCCAGGC GAGAATTTGT 
    GGTCTCAACG CGGCGCTCAC AATTCAACTG TGCGGGCATC TCCTCCACAT GGTCAATGCC 
    TTTGTGTTCA ACAGCTTGGT CTCAGTCAAC CTTCCAGCTG CCCAGTTGAA TACTCGCCTC 
    GGAAAGTGTC TTCAGTATCG GATAGAGTCG ATTCACAGAT TCTGCGAACG AATGGGCGTC 
    GAGATGGCCG CCGAGTGCCA TTTGGATAGA TCCCGTCAGG CCGCTAACCT TCTCGCCACG 
    CAGAAGAATG ATGTTGCCGC GTTGGGATCG ACGTGCTATA AGCTGAACAG CCTCCAAGTG 
    GTTCATATCC TGTCGGGATT CGAACCAGAC GACGGCGAGA TTCCATGTGA CGATGACGTC 
    GTTGATCGGA TCTGTAGACT CGCTGAAAAA CAGGCGGATA AGCTGACGTT GGAGGATGGG 
    CAGCGCGTGA CACTGGAGGA GTCCGACGAG CTGAACCTTC CGTTCCTGAT CCCTCAGGAT 
    GGTTACTTTG TCGAGCAGTT CCGAACGGTG CCAGATGGTC TTTGGCAGTA CTTGCAGCAA 
    CTTCAGCATA GGCGCCTCTG TCATACGGTG TCGCTTACAC CGGCACCGCC GGCTGCGATG 
    ACCCATTCGA TGCATGCGAT GCATATTGGA GCAAGCGATG CTCATTATCA TCAGCATCAG 
    CATCAGCAGC AGCAGAACCC GATTGTGAGA AGTGTGTCGC AGACTACACT GACCAGTATT 
    CCGACTACAC AATTTGGCGA CAGTTTTGGA AGTGCCGTGT CGGATACGAT ATCGAAGGTG 
    ACACTGCGGA AGAATGGAGG AGGCATCGGA TTGAGTATTG TCGCTGCTCA GGGTGTTGGT 
    GATCGTCAAA TGGGAATTTA TGTGAAGAAG GTTGTCGAAG GAACACCGGC TGCACACGAT 
    GGACGACTCG AGACTGGTGA TCAGTTATTG AGTGTTAACG GACATTCGTT AATTGGAATT 
    TCACAGGAAG ACGCCGCCCG TCTCATGACC CAAAGTGGAA ACGAGGTGCA TTTCGACGTC 
    CGGAAAAGAG CCGCTCACCG TAACGGGCTC AGCAATTGGC TCGGATCCCA AACACCGACA 
    ACTTCAAATT TCATTGCACC ATCTCCATCT ACAGTACCCC CGCCGAGTTA TTCACATGTT 
    GGACGGACTC CGTCTTATGA TGGACAACCA CCACCGAGTT ATAATCACAG AAATGGTGGA 
    GCCAGCGGAG CAGGAGGATC ATCTGGAAGA AATAGTGCTT TCGTAGCACC ACTTTCGAAT 
    TCATCATCAA ATGCGTCGAG TAGTCATATG CAACAACACT ATGCTCATCA TAATATGATG 
    CATAATGGCA ATTTGAGTGC TGCTCCGGGA GCTCCCGCGG GGGATCCGAA ACATTATCGA 
    AGCTCGTCGG CTAGTGATTT GCAGCACGAT CCAAACGCCT CATTCTCTCA ATCATCAATG 
    ACTGGAAGTG CGAGAAGTTC AGTGTTTGAC AAGCTTCCAT CTCACTACCG TCACTCGACA 
    CGGCCTACAG TAATCCAGCC AACACGTCCA AGTGCTCAAT CTCCATCGGC TCTTCGAAAA 
    GCGGCCGCCG CCTCGCCGGT CAGCAATCTG TACCGTCCGA CGTCGGCGTC GAATCTGTTT 
    GCACCGCCCA GAAATTCGGG CGGTCTCGTC GGATATTCGA GTAACGCGGC ACGGGAGTCG 
    ACACAGGATA TTCATCGAGC GCTAAATGAC CTGGAACTCA ACTCACCGCT CGCCAGATCC 
    ACGCCGAATG AGGAGTACCG TATCCCGCCG CCTAGAGTAC CTACAGTAAC CTCCACCTCC 
    TCCAACTATG CCCCTCTTCA TTCTATTCGA CCGGCAAGCA TGTATGGATA TCAGAGCCCG 
    AGCTCTTCTA CTACAGTAAT CCCGGCTCCA AAAGGAGTGG CTTCTTCCTC TAATAACACT 
    TCTCCCCGAG TGAATGTGAC GTTGGGTGGA GCATTGAGCT CATCGAGATC TCATAATAGT 
    CTTTCACAGG AGCCAGCCAT TGTTATCAGG CCTGTCGACC TGAATATGCC GAAAGCGACA 
    CCACCGGCGT CGATTCGTGG AGCTGTGCCG ACGGCGATTA CACAGGCTCT TCAACTTCAA 
    CCAAACGGCG GTGGAGCAGT ACTTCGTCAG GTTCGGCCAG CATCCGGGCT CCGCGAGCGA 
    CTTCAGGACG CTGATGTTCG GAATTTGGAG AAAACGAACA CAGCACTGAT GAGCTATGAA 
    GCAGTGAATG AGGAGCTCGA TCGACTCGAC GCTAAGCAGA ATACGATGAC AATTGACGAG 
    CAACGGAGAT ATCGAGAGCT ACTGAACGTT GTCGCCGAGC AGAGCCGAGC AAGGAAGCTG 
    CTCTCGGAGA TCAGATCACC ACCTACATCT TATCAAGAAC GTGAAATTCC GATTCATGTG 
    CAAAAAGAGC AGCAGCAGCA CCGGCGGGAA ACTGTGATTG ATGATATTCC ACACTCGAGC 
    TCAAGTGTTG AGAATGTGTC ACCTGTGAGC CCGCCGGAAA GTCGAAAAGT TCAATTTATT 
    GATGAAGTTG TGCAGTATCA GACGCCGACC AGCGGAGCCA ATCACGCTTT CTTTGATGAT 
    GTGCCGGTTT TGGATTCGCC TGGGATCGTC GGCACCAACG AAATCTACCG TGATCCCCGC 
    CAACGTCGGC TCAACGAGCT CCAAGACCGC AATCGCAGCT CGGAAAATGG AGCGGCGGAC 
    GGCGCGAAGC TCGGCTTCCG CGACAAGCAG CGCCTATTCG CACGGCAAAT CGGCGAGGAC 
    ACGGCACCAC GCCAACGAAT GAATGAGAGC AGTGCTCAGC GGCTCATTGA GACGGACATT 
    GCCGCCACCC CGCAAAAGCC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306407.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293336.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336168
    Clone ID Related Accession (Same CDS sequence) NM_001306407.1 , NM_001306407.2
    Accession Version NM_001306407.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCCGGCT CATCAGTCTC ATCATCACGG CAGGAGGATC TTATGATGCA TTCCAATAAT 
    AACATCCTGC CACCGTACTC TACAAAATCC GCGCAGGAAC CCCTCCAACT GCCGCTCTCA 
    ATTCAAATCG GCGACTACAA TGTATGGGAT TTCCTGGCCG AAGTGATGTC GAATCGTGCG 
    GCAACCTCAT CGGAAATGTC CACATTCCGC CTATCGCCTG CCTACTCTCT CTACCTGGCA 
    CTTCGATCAT TCCACACACA CAATAAACCG TACTCTGTGC AATTCTTCTC ACGGATCGAG 
    TCGAATTTCA AGCAAATCGC TCAGGAATGC TCGTCGAAGG ATGATCTGAT CTTTTGGCTT 
    GCCAACGCAT CGGAATTCAA TCATCTTGTC GAGCGGGACA CTGAGCTGAA AGCGTCGAGA 
    TATGGCAAAA TATCTGTGGA AATTGAGAGA ATATTCCGGC GATTGGTTGC AGTGCTTCAA 
    AGTGTCATCC GTCCGGCGGC GCGGGCTCTG TTGGACATTC ATCTGAATGA TCACGACGCG 
    ACCGGCGATG TGATTCATCT TCTCGACACC ACGATGCGCC AGGCGAGAAT TTGTGGTCTC 
    AACGCGGCGC TCACAATTCA ACTGTGCGGG CATCTCCTCC ACATGGTCAA TGCCTTTGTG 
    TTCAACAGCT TGGTCTCAGT CAACCTTCCA GCTGCCCAGT TGAATACTCG CCTCGGAAAG 
    TGTCTTCAGT ATCGGATAGA GTCGATTCAC AGATTCTGCG AACGAATGGG CGTCGAGATG 
    GCCGCCGAGT GCCATTTGGA TAGATCCCGT CAGGCCGCTA ACCTTCTCGC CACGCAGAAG 
    AATGATGTTG CCGCGTTGGG ATCGACGTGC TATAAGCTGA ACAGCCTCCA AGTGGTTCAT 
    ATCCTGTCGG GATTCGAACC AGACGACGGC GAGATTCCAT GTGACGATGA CGTCGTTGAT 
    CGGATCTGTA GACTCGCTGA AAAACAGGCG GATAAGCTGA CGTTGGAGGA TGGGCAGCGC 
    GTGACACTGG AGGAGTCCGA CGAGCTGAAC CTTCCGTTCC TGATCCCTCA GGATGGTTAC 
    TTTGTCGAGC AGTTCCGAAC GGTGCCAGAT GGTCTTTGGC AGTACTTGCA GCAACTTCAG 
    CATAGGCGCC TCTGTCATAC GGTGTCGCTT ACACCGGCAC CGCCGGCTGC GATGACCCAT 
    TCGATGCATG CGATGCATAT TGGAGCAAGC GATGCTCATT ATCATCAGCA TCAGCATCAG 
    CAGCAGCAGA ACCCGATTGT GAGAAGTGTG TCGCAGACTA CACTGACCAG TATTCCGACT 
    ACACAATTTG GCGACAGTTT TGGAAGTGCC GTGTCGGATA CGATATCGAA GGTGACACTG 
    CGGAAGAATG GAGGAGGCAT CGGATTGAGT ATTGTCGCTG CTCAGGGTGT TGGTGATCGT 
    CAAATGGGAA TTTATGTGAA GAAGGTTGTC GAAGGAACAC CGGCTGCACA CGATGGACGA 
    CTCGAGACTG GTGATCAGTT ATTGAGTGTT AACGGACATT CGTTAATTGG AATTTCACAG 
    GAAGACGCCG CCCGTCTCAT GACCCAAAGT GGAAACGAGG TGCATTTCGA CGTCCGGAAA 
    AGAGCCGCTC ACCGTAACGG GCTCAGCAAT TGGCTCGGAT CCCAAACACC GACAACTTCA 
    AATTTCATTG CACCATCTCC ATCTACAGTA CCCCCGCCGA GTTATTCACA TGTTGGACGG 
    ACTCCGTCTT ATGATGGACA ACCACCACCG AGTTATAATC ACAGAAATGG TGGAGCCAGC 
    GGAGCAGGAG GATCATCTGG AAGAAATAGT GCTTTCGTAG CACCACTTTC GAATTCATCA 
    TCAAATGCGT CGAGTAGTCA TATGCAACAA CACTATGCTC ATCATAATAT GATGCATAAT 
    GGCAATTTGA GTGCTGCTCC GGGAGCTCCC GCGGGGGATC CGAAACATTA TCGAAGCTCG 
    TCGGCTAGTG ATTTGCAGCA CGATCCAAAC GCCTCATTCT CTCAATCATC AATGACTGGA 
    AGTGCGAGAA GTTCAGTGTT TGACAAGCTT CCATCTCACT ACCGTCACTC GACACGGCCT 
    ACAGTAATCC AGCCAACACG TCCAAGTGCT CAATCTCCAT CGGCTCTTCG AAAAGCGGCC 
    GCCGCCTCGC CGGTCAGCAA TCTGTACCGT CCGACGTCGG CGTCGAATCT GTTTGCACCG 
    CCCAGAAATT CGGGCGGTCT CGTCGGATAT TCGAGTAACG CGGCACGGGA GTCGACACAG 
    GATATTCATC GAGCGCTAAA TGACCTGGAA CTCAACTCAC CGCTCGCCAG ATCCACGCCG 
    AATGAGGAGT ACCGTATCCC GCCGCCTAGA GTACCTACAG TAACCTCCAC CTCCTCCAAC 
    TATGCCCCTC TTCATTCTAT TCGACCGGCA AGCATGTATG GATATCAGAG CCCGAGCTCT 
    TCTACTACAG TAATCCCGGC TCCAAAAGGA GTGGCTTCTT CCTCTAATAA CACTTCTCCC 
    CGAGTGAATG TGACGTTGGG TGGAGCATTG AGCTCATCGA GATCTCATAA TAGTCTTTCA 
    CAGGAGCCAG CCATTGTTAT CAGGCCTGTC GACCTGAATA TGCCGAAAGC GACACCACCG 
    GCGTCGATTC GTGGAGCTGT GCCGACGGCG ATTACACAGG CTCTTCAACT TCAACCAAAC 
    GGCGGTGGAG CAGTACTTCG TCAGGTTCGG CCAGCATCCG GGCTCCGCGA GCGACTTCAG 
    GACGCTGATG TTCGGAATTT GGAGAAAACG AACACAGCAC TGATGAGCTA TGAAGCAGTG 
    AATGAGGAGC TCGATCGACT CGACGCTAAG CAGAATACGA TGACAATTGA CGAGCAACGG 
    AGATATCGAG AGCTACTGAA CGTTGTCGCC GAGCAGAGCC GAGCAAGGAA GCTGCTCTCG 
    GAGATCAGAT CACCACCTAC ATCTTATCAA GAACGTGAAA TTCCGATTCA TGTGCAAAAA 
    GAGCAGCAGC AGCACCGGCG GGAAACTGTG ATTGATGATA TTCCACACTC GAGCTCAAGT 
    GTTGAGAATG TGTCACCTGT GAGCCCGCCG GAAAGTCGAA AAGTTCAATT TATTGATGAA 
    GTTGTGCAGT ATCAGACGCC GACCAGCGGA GCCAATCACG CTTTCTTTGA TGATGTGCCG 
    GTTTTGGATT CGCCTGGGAT CGTCGGCACC AACGAAATCT ACCGTGATCC CCGCCAACGT 
    CGGCTCAACG AGCTCCAAGA CCGCAATCGC AGCTCGGAAA ATGGAGCGGC GGACGGCGCG 
    AAGCTCGGCT TCCGCGACAA GCAGCGCCTA TTCGCACGGC AAATCGGCGA GGACACGGCA 
    CCACGCCAAC GAATGAATGA GAGCAGTGCT CAGCGGCTCA TTGAGACGGA CATTGCCGCC 
    ACCCCGCAAA AGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293336.1
    CDS1..3438
    Translation

    Target ORF information:

    RefSeq Version NM_001306407.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306407.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCCGGCT CATCAGTCTC ATCATCACGG CAGGAGGATC TTATGATGCA TTCCAATAAT 
    AACATCCTGC CACCGTACTC TACAAAATCC GCGCAGGAAC CCCTCCAACT GCCGCTCTCA 
    ATTCAAATCG GCGACTACAA TGTATGGGAT TTCCTGGCCG AAGTGATGTC GAATCGTGCG 
    GCAACCTCAT CGGAAATGTC CACATTCCGC CTATCGCCTG CCTACTCTCT CTACCTGGCA 
    CTTCGATCAT TCCACACACA CAATAAACCG TACTCTGTGC AATTCTTCTC ACGGATCGAG 
    TCGAATTTCA AGCAAATCGC TCAGGAATGC TCGTCGAAGG ATGATCTGAT CTTTTGGCTT 
    GCCAACGCAT CGGAATTCAA TCATCTTGTC GAGCGGGACA CTGAGCTGAA AGCGTCGAGA 
    TATGGCAAAA TATCTGTGGA AATTGAGAGA ATATTCCGGC GATTGGTTGC AGTGCTTCAA 
    AGTGTCATCC GTCCGGCGGC GCGGGCTCTG TTGGACATTC ATCTGAATGA TCACGACGCG 
    ACCGGCGATG TGATTCATCT TCTCGACACC ACGATGCGCC AGGCGAGAAT TTGTGGTCTC 
    AACGCGGCGC TCACAATTCA ACTGTGCGGG CATCTCCTCC ACATGGTCAA TGCCTTTGTG 
    TTCAACAGCT TGGTCTCAGT CAACCTTCCA GCTGCCCAGT TGAATACTCG CCTCGGAAAG 
    TGTCTTCAGT ATCGGATAGA GTCGATTCAC AGATTCTGCG AACGAATGGG CGTCGAGATG 
    GCCGCCGAGT GCCATTTGGA TAGATCCCGT CAGGCCGCTA ACCTTCTCGC CACGCAGAAG 
    AATGATGTTG CCGCGTTGGG ATCGACGTGC TATAAGCTGA ACAGCCTCCA AGTGGTTCAT 
    ATCCTGTCGG GATTCGAACC AGACGACGGC GAGATTCCAT GTGACGATGA CGTCGTTGAT 
    CGGATCTGTA GACTCGCTGA AAAACAGGCG GATAAGCTGA CGTTGGAGGA TGGGCAGCGC 
    GTGACACTGG AGGAGTCCGA CGAGCTGAAC CTTCCGTTCC TGATCCCTCA GGATGGTTAC 
    TTTGTCGAGC AGTTCCGAAC GGTGCCAGAT GGTCTTTGGC AGTACTTGCA GCAACTTCAG 
    CATAGGCGCC TCTGTCATAC GGTGTCGCTT ACACCGGCAC CGCCGGCTGC GATGACCCAT 
    TCGATGCATG CGATGCATAT TGGAGCAAGC GATGCTCATT ATCATCAGCA TCAGCATCAG 
    CAGCAGCAGA ACCCGATTGT GAGAAGTGTG TCGCAGACTA CACTGACCAG TATTCCGACT 
    ACACAATTTG GCGACAGTTT TGGAAGTGCC GTGTCGGATA CGATATCGAA GGTGACACTG 
    CGGAAGAATG GAGGAGGCAT CGGATTGAGT ATTGTCGCTG CTCAGGGTGT TGGTGATCGT 
    CAAATGGGAA TTTATGTGAA GAAGGTTGTC GAAGGAACAC CGGCTGCACA CGATGGACGA 
    CTCGAGACTG GTGATCAGTT ATTGAGTGTT AACGGACATT CGTTAATTGG AATTTCACAG 
    GAAGACGCCG CCCGTCTCAT GACCCAAAGT GGAAACGAGG TGCATTTCGA CGTCCGGAAA 
    AGAGCCGCTC ACCGTAACGG GCTCAGCAAT TGGCTCGGAT CCCAAACACC GACAACTTCA 
    AATTTCATTG CACCATCTCC ATCTACAGTA CCCCCGCCGA GTTATTCACA TGTTGGACGG 
    ACTCCGTCTT ATGATGGACA ACCACCACCG AGTTATAATC ACAGAAATGG TGGAGCCAGC 
    GGAGCAGGAG GATCATCTGG AAGAAATAGT GCTTTCGTAG CACCACTTTC GAATTCATCA 
    TCAAATGCGT CGAGTAGTCA TATGCAACAA CACTATGCTC ATCATAATAT GATGCATAAT 
    GGCAATTTGA GTGCTGCTCC GGGAGCTCCC GCGGGGGATC CGAAACATTA TCGAAGCTCG 
    TCGGCTAGTG ATTTGCAGCA CGATCCAAAC GCCTCATTCT CTCAATCATC AATGACTGGA 
    AGTGCGAGAA GTTCAGTGTT TGACAAGCTT CCATCTCACT ACCGTCACTC GACACGGCCT 
    ACAGTAATCC AGCCAACACG TCCAAGTGCT CAATCTCCAT CGGCTCTTCG AAAAGCGGCC 
    GCCGCCTCGC CGGTCAGCAA TCTGTACCGT CCGACGTCGG CGTCGAATCT GTTTGCACCG 
    CCCAGAAATT CGGGCGGTCT CGTCGGATAT TCGAGTAACG CGGCACGGGA GTCGACACAG 
    GATATTCATC GAGCGCTAAA TGACCTGGAA CTCAACTCAC CGCTCGCCAG ATCCACGCCG 
    AATGAGGAGT ACCGTATCCC GCCGCCTAGA GTACCTACAG TAACCTCCAC CTCCTCCAAC 
    TATGCCCCTC TTCATTCTAT TCGACCGGCA AGCATGTATG GATATCAGAG CCCGAGCTCT 
    TCTACTACAG TAATCCCGGC TCCAAAAGGA GTGGCTTCTT CCTCTAATAA CACTTCTCCC 
    CGAGTGAATG TGACGTTGGG TGGAGCATTG AGCTCATCGA GATCTCATAA TAGTCTTTCA 
    CAGGAGCCAG CCATTGTTAT CAGGCCTGTC GACCTGAATA TGCCGAAAGC GACACCACCG 
    GCGTCGATTC GTGGAGCTGT GCCGACGGCG ATTACACAGG CTCTTCAACT TCAACCAAAC 
    GGCGGTGGAG CAGTACTTCG TCAGGTTCGG CCAGCATCCG GGCTCCGCGA GCGACTTCAG 
    GACGCTGATG TTCGGAATTT GGAGAAAACG AACACAGCAC TGATGAGCTA TGAAGCAGTG 
    AATGAGGAGC TCGATCGACT CGACGCTAAG CAGAATACGA TGACAATTGA CGAGCAACGG 
    AGATATCGAG AGCTACTGAA CGTTGTCGCC GAGCAGAGCC GAGCAAGGAA GCTGCTCTCG 
    GAGATCAGAT CACCACCTAC ATCTTATCAA GAACGTGAAA TTCCGATTCA TGTGCAAAAA 
    GAGCAGCAGC AGCACCGGCG GGAAACTGTG ATTGATGATA TTCCACACTC GAGCTCAAGT 
    GTTGAGAATG TGTCACCTGT GAGCCCGCCG GAAAGTCGAA AAGTTCAATT TATTGATGAA 
    GTTGTGCAGT ATCAGACGCC GACCAGCGGA GCCAATCACG CTTTCTTTGA TGATGTGCCG 
    GTTTTGGATT CGCCTGGGAT CGTCGGCACC AACGAAATCT ACCGTGATCC CCGCCAACGT 
    CGGCTCAACG AGCTCCAAGA CCGCAATCGC AGCTCGGAAA ATGGAGCGGC GGACGGCGCG 
    AAGCTCGGCT TCCGCGACAA GCAGCGCCTA TTCGCACGGC AAATCGGCGA GGACACGGCA 
    CCACGCCAAC GAATGAATGA GAGCAGTGCT CAGCGGCTCA TTGAGACGGA CATTGCCGCC 
    ACCCCGCAAA AGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306401.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293330.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336162
    Clone ID Related Accession (Same CDS sequence) NM_001306401.1 , NM_001306401.2
    Accession Version NM_001306401.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306401.1. COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCAGACC TCCGTGAAAT CGACGGACGG CCCATTCCAC CAACAGAGTG CCCGCTTTTC 
    GAGATGACTG CTCGAAGTGG AAACGGCGAG AATGGATTCG ATTCGTTTTT GGCGATCAAG 
    CGGAAACCGC ATGATTATCA AGTCCCCTCC ACCTCAGTTG TTGCTCCATC ATCAGTCACC 
    CTACCAATGT CTCCATTAAC ATCACTTCCA CCACAAATTC ACAATCTTCC AGTGCTTATT 
    GTGATGAATA ATGATGGTCA CTTGTCAACA CACCGAATAA CGTTACACGA GGGAGTAACA 
    GAGGTTGGAA GTGACAGTCA AATGTCCAAT TTCTCACAGC ATAATATTTA TCTCGATGGT 
    CCGGATATTC GTGGACGCCA CGCGGCAATC GCTTTCATGG AAGGCGTCGT CACACTAACA 
    CCATCCACTC GTGATGCCTA CCTAGAGGTG AACGGCCATC AACTGATGCA GACGGAGATC 
    CTCCGGGACG GTGATCACAT TCGCATCGGA GCCGATCATT CGTTCAAGTT TAGCACGTCG 
    TCGTCACAAG CAAATCAGAT TAACAGTACT AATATCAGCA ACTCGAACCA ACGACACTTC 
    CAACAACAGA CATTTTATCC GGAAATGGCT GATTCACGAG CTTCAACTGC CAGGTGTGCA 
    CCATCGGATT ACTGTAGTCT ACCACAACAG AACGATTATT ATCCGCCGAG CATGTCCGGC 
    TCATCAGTCT CATCATCACG GCAGGAGGAT CTTATGATGC ATTCCAATAA TAACATCCTG 
    CCACCGTACT CTACAAAATC CGCGCAGGAA CCCCTCCAAC TGCCGCTCTC AATTCAAATC 
    GGCGACTACA ATGTATGGGA TTTCCTGGCC GAAGTGATGT CGAATCGTGC GGCAACCTCA 
    TCGGAAATGT CCACATTCCG CCTATCGCCT GCCTACTCTC TCTACCTGGC ACTTCGATCA 
    TTCCACACAC ACAATAAACC GTACTCTGTG CAATTCTTCT CACGGATCGA GTCGAATTTC 
    AAGCAAATCG CTCAGGAATG CTCGTCGAAG GATGATCTGA TCTTTTGGCT TGCCAACGCA 
    TCGGAATTCA ATCATCTTGT CGAGCGGGAC ACTGAGCTGA AAGCGTCGAG ATATGGCAAA 
    ATATCTGTGG AAATTGAGAG AATATTCCGG CGATTGGTTG CAGTGCTTCA AAGTGTCATC 
    CGTCCGGCGG CGCGGGCTCT GTTGGACATT CATCTGAATG ATCACGACGC GACCGGCGAT 
    GTGATTCATC TTCTCGACAC CACGATGCGC CAGGCGAGAA TTTGTGGTCT CAACGCGGCG 
    CTCACAATTC AACTGTGCGG GCATCTCCTC CACATGGTCA ATGCCTTTGT GTTCAACAGC 
    TTGGTCTCAG TCAACCTTCC AGCTGCCCAG TTGAATACTC GCCTCGGAAA GTGTCTTCAG 
    TATCGGATAG AGTCGATTCA CAGATTCTGC GAACGAATGG GCGTCGAGAT GGCCGCCGAG 
    TGCCATTTGG ATAGATCCCG TCAGGCCGCT AACCTTCTCG CCACGCAGAA GAATGATGTT 
    GCCGCGTTGG GATCGACGTG CTATAAGCTG AACAGCCTCC AAGTGGTTCA TATCCTGTCG 
    GGATTCGAAC CAGACGACGG CGAGATTCCA TGTGACGATG ACGTCGTTGA TCGGATCTGT 
    AGACTCGCTG AAAAACAGGC GGATAAGCTG ACGTTGGAGG ATGGGCAGCG CGTGACACTG 
    GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC CTGATCCCTC AGGATGGTTA CTTTGTCGAG 
    CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG CAGTACTTGC AGCAACTTCA GCATAGGCGC 
    CTCTGTCATA CGGTGTCGCT TACACCGGCA CCGCCGGCTG CGATGACCCA TTCGATGCAT 
    GCGATGCATA TTGGAGCAAG CGATGCTCAT TATCATCAGC ATCAGCATCA GCAGCAGCAG 
    AACCCGATTG TGAGAAGTGT GTCGCAGACT ACACTGACCA GTATTCCGAC TACACAATTT 
    GGCGACAGTT TTGGAAGTGC CGTGTCGGAT ACGATATCGA AGGTGACACT GCGGAAGAAT 
    GGAGGAGGCA TCGGATTGAG TATTGTCGCT GCTCAGGGTG TTGGTGATCG TCAAATGGGA 
    ATTTATGTGA AGAAGGTTGT CGAAGGAACA CCGGCTGCAC ACGATGGACG ACTCGAGACT 
    GGTGATCAGT TATTGAGTGT TAACGGACAT TCGTTAATTG GAATTTCACA GGAAGACGCC 
    GCCCGTCTCA TGACCCAAAG TGGAAACGAG GTGCATTTCG ACGTCCGGAA AAGAGCCGCT 
    CACCGTAACG GGCTCAGCAA TTGGCTCGGA TCCCAAACAC CGACAACTTC AAATTTCATT 
    GCACCATCTC CATCTACAGT ACCCCCGCCG AGTTATTCAC ATGTTGGACG GACTCCGTCT 
    TATGATGGAC AACCACCACC GAGTTATAAT CACAGAAATG GTGGAGCCAG CGGAGCAGGA 
    GGATCATCTG GAAGAAATAG TGCTTTCGTA GCACCACTTT CGAATTCATC ATCAAATGCG 
    TCGAGTAGTC ATATGCAACA ACACTATGCT CATCATAATA TGATGCATAA TGGCAATTTG 
    AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT CCGAAACATT ATCGAAGCTC GTCGGCTAGT 
    GATTTGCAGC ACGATCCAAA CGCCTCATTC TCTCAATCAT CAATGACTGG AAGTGCGAGA 
    AGTTCAGTGT TTGACAAGCT TCCATCTCAC TACCGTCACT CGACACGGCC TACAGTAATC 
    CAGCCAACAC GTCCAAGTGC TCAATCTCCA TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG 
    CCGGTCAGCA ATCTGTACCG TCCGACGTCG GCGTCGAATC TGTTTGCACC GCCCAGAAAT 
    TCGGGCGGTC TCGTCGGATA TTCGAGTAAC GCGGCACGGG AGTCGACACA GGATATTCAT 
    CGAGCGCTAA ATGACCTGGA ACTCAACTCA CCGCTCGCCA GATCCACGCC GAATGAGGAG 
    TACCGTATCC CGCCGCCTAG AGTACCTACA GTAACCTCCA CCTCCTCCAA CTATGCCCCT 
    CTTCATTCTA TTCGACCGGC AAGCATGTAT GGATATCAGA GCCCGAGCTC TTCTACTACA 
    GTAATCCCGG CTCCAAAAGG AGTGGCTTCT TCCTCTAATA ACACTTCTCC CCGAGTGAAT 
    GTGACGTTGG GTGGAGCATT GAGCTCATCG AGATCTCATA ATAGTCTTTC ACAGGAGCCA 
    GCCATTGTTA TCAGGCCTGT CGACCTGAAT ATGCCGAAAG CGACACCACC GGCGTCGATT 
    CGTGGAGCTG TGCCGACGGC GATTACACAG GCTCTTCAAC TTCAACCAAA CGGCGGTGGA 
    GCAGTACTTC GTCAGGTTCG GCCAGCATCC GGGCTCCGCG AGCGACTTCA GGACGCTGAT 
    GTTCGGAATT TGGAGAAAAC GAACACAGCA CTGATGAGCT ATGAAGCAGT GAATGAGGAG 
    CTCGATCGAC TCGACGCTAA GCAGAATACG ATGACAATTG ACGAGCAACG GAGATATCGA 
    GAGCTACTGA ACGTTGTCGC CGAGCAGAGC CGAGCAAGGA AGCTGCTCTC GGAGATCAGA 
    TCACCACCTA CATCTTATCA AGAACGTGAA ATTCCGATTC ATGTGCAAAA AGAGCAGCAG 
    CAGCACCGGC GGGAAACTGT GATTGATGAT ATTCCACACT CGAGCTCAAG TGTTGAGAAT 
    GTGTCACCTG TGAGCCCGCC GGAAAGTCGA AAAGTTCAAT TTATTGATGA AGTTGTGCAG 
    TATCAGACGC CGACCAGCGG AGCCAATCAC GCTTTCTTTG ATGATGTGCC GGTTTTGGAT 
    TCGCCTGGGA TCGTCGGCAC CAACGAAATC TACCGTGATC CCCGCCAACG TCGGCTCAAC 
    GAGCTCCAAG ACCGCAATCG CAGCTCGGAA AATGGAGCGG CGGACGGCGC GAAGCTCGGC 
    TTCCGCGACA AGCAGCGCCT ATTCGCACGG CAAATCGGCG AGGACACGGC ACCACGCCAA 
    CGAATGAATG AGAGCAGTGC TCAGCGGCTC ATTGAGACGG ACATTGCCGC CACCCCGCAA 
    AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293330.1
    CDS1..4149
    Translation

    Target ORF information:

    RefSeq Version NM_001306401.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306401.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCAGACC TCCGTGAAAT CGACGGACGG CCCATTCCAC CAACAGAGTG CCCGCTTTTC 
    GAGATGACTG CTCGAAGTGG AAACGGCGAG AATGGATTCG ATTCGTTTTT GGCGATCAAG 
    CGGAAACCGC ATGATTATCA AGTCCCCTCC ACCTCAGTTG TTGCTCCATC ATCAGTCACC 
    CTACCAATGT CTCCATTAAC ATCACTTCCA CCACAAATTC ACAATCTTCC AGTGCTTATT 
    GTGATGAATA ATGATGGTCA CTTGTCAACA CACCGAATAA CGTTACACGA GGGAGTAACA 
    GAGGTTGGAA GTGACAGTCA AATGTCCAAT TTCTCACAGC ATAATATTTA TCTCGATGGT 
    CCGGATATTC GTGGACGCCA CGCGGCAATC GCTTTCATGG AAGGCGTCGT CACACTAACA 
    CCATCCACTC GTGATGCCTA CCTAGAGGTG AACGGCCATC AACTGATGCA GACGGAGATC 
    CTCCGGGACG GTGATCACAT TCGCATCGGA GCCGATCATT CGTTCAAGTT TAGCACGTCG 
    TCGTCACAAG CAAATCAGAT TAACAGTACT AATATCAGCA ACTCGAACCA ACGACACTTC 
    CAACAACAGA CATTTTATCC GGAAATGGCT GATTCACGAG CTTCAACTGC CAGGTGTGCA 
    CCATCGGATT ACTGTAGTCT ACCACAACAG AACGATTATT ATCCGCCGAG CATGTCCGGC 
    TCATCAGTCT CATCATCACG GCAGGAGGAT CTTATGATGC ATTCCAATAA TAACATCCTG 
    CCACCGTACT CTACAAAATC CGCGCAGGAA CCCCTCCAAC TGCCGCTCTC AATTCAAATC 
    GGCGACTACA ATGTATGGGA TTTCCTGGCC GAAGTGATGT CGAATCGTGC GGCAACCTCA 
    TCGGAAATGT CCACATTCCG CCTATCGCCT GCCTACTCTC TCTACCTGGC ACTTCGATCA 
    TTCCACACAC ACAATAAACC GTACTCTGTG CAATTCTTCT CACGGATCGA GTCGAATTTC 
    AAGCAAATCG CTCAGGAATG CTCGTCGAAG GATGATCTGA TCTTTTGGCT TGCCAACGCA 
    TCGGAATTCA ATCATCTTGT CGAGCGGGAC ACTGAGCTGA AAGCGTCGAG ATATGGCAAA 
    ATATCTGTGG AAATTGAGAG AATATTCCGG CGATTGGTTG CAGTGCTTCA AAGTGTCATC 
    CGTCCGGCGG CGCGGGCTCT GTTGGACATT CATCTGAATG ATCACGACGC GACCGGCGAT 
    GTGATTCATC TTCTCGACAC CACGATGCGC CAGGCGAGAA TTTGTGGTCT CAACGCGGCG 
    CTCACAATTC AACTGTGCGG GCATCTCCTC CACATGGTCA ATGCCTTTGT GTTCAACAGC 
    TTGGTCTCAG TCAACCTTCC AGCTGCCCAG TTGAATACTC GCCTCGGAAA GTGTCTTCAG 
    TATCGGATAG AGTCGATTCA CAGATTCTGC GAACGAATGG GCGTCGAGAT GGCCGCCGAG 
    TGCCATTTGG ATAGATCCCG TCAGGCCGCT AACCTTCTCG CCACGCAGAA GAATGATGTT 
    GCCGCGTTGG GATCGACGTG CTATAAGCTG AACAGCCTCC AAGTGGTTCA TATCCTGTCG 
    GGATTCGAAC CAGACGACGG CGAGATTCCA TGTGACGATG ACGTCGTTGA TCGGATCTGT 
    AGACTCGCTG AAAAACAGGC GGATAAGCTG ACGTTGGAGG ATGGGCAGCG CGTGACACTG 
    GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC CTGATCCCTC AGGATGGTTA CTTTGTCGAG 
    CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG CAGTACTTGC AGCAACTTCA GCATAGGCGC 
    CTCTGTCATA CGGTGTCGCT TACACCGGCA CCGCCGGCTG CGATGACCCA TTCGATGCAT 
    GCGATGCATA TTGGAGCAAG CGATGCTCAT TATCATCAGC ATCAGCATCA GCAGCAGCAG 
    AACCCGATTG TGAGAAGTGT GTCGCAGACT ACACTGACCA GTATTCCGAC TACACAATTT 
    GGCGACAGTT TTGGAAGTGC CGTGTCGGAT ACGATATCGA AGGTGACACT GCGGAAGAAT 
    GGAGGAGGCA TCGGATTGAG TATTGTCGCT GCTCAGGGTG TTGGTGATCG TCAAATGGGA 
    ATTTATGTGA AGAAGGTTGT CGAAGGAACA CCGGCTGCAC ACGATGGACG ACTCGAGACT 
    GGTGATCAGT TATTGAGTGT TAACGGACAT TCGTTAATTG GAATTTCACA GGAAGACGCC 
    GCCCGTCTCA TGACCCAAAG TGGAAACGAG GTGCATTTCG ACGTCCGGAA AAGAGCCGCT 
    CACCGTAACG GGCTCAGCAA TTGGCTCGGA TCCCAAACAC CGACAACTTC AAATTTCATT 
    GCACCATCTC CATCTACAGT ACCCCCGCCG AGTTATTCAC ATGTTGGACG GACTCCGTCT 
    TATGATGGAC AACCACCACC GAGTTATAAT CACAGAAATG GTGGAGCCAG CGGAGCAGGA 
    GGATCATCTG GAAGAAATAG TGCTTTCGTA GCACCACTTT CGAATTCATC ATCAAATGCG 
    TCGAGTAGTC ATATGCAACA ACACTATGCT CATCATAATA TGATGCATAA TGGCAATTTG 
    AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT CCGAAACATT ATCGAAGCTC GTCGGCTAGT 
    GATTTGCAGC ACGATCCAAA CGCCTCATTC TCTCAATCAT CAATGACTGG AAGTGCGAGA 
    AGTTCAGTGT TTGACAAGCT TCCATCTCAC TACCGTCACT CGACACGGCC TACAGTAATC 
    CAGCCAACAC GTCCAAGTGC TCAATCTCCA TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG 
    CCGGTCAGCA ATCTGTACCG TCCGACGTCG GCGTCGAATC TGTTTGCACC GCCCAGAAAT 
    TCGGGCGGTC TCGTCGGATA TTCGAGTAAC GCGGCACGGG AGTCGACACA GGATATTCAT 
    CGAGCGCTAA ATGACCTGGA ACTCAACTCA CCGCTCGCCA GATCCACGCC GAATGAGGAG 
    TACCGTATCC CGCCGCCTAG AGTACCTACA GTAACCTCCA CCTCCTCCAA CTATGCCCCT 
    CTTCATTCTA TTCGACCGGC AAGCATGTAT GGATATCAGA GCCCGAGCTC TTCTACTACA 
    GTAATCCCGG CTCCAAAAGG AGTGGCTTCT TCCTCTAATA ACACTTCTCC CCGAGTGAAT 
    GTGACGTTGG GTGGAGCATT GAGCTCATCG AGATCTCATA ATAGTCTTTC ACAGGAGCCA 
    GCCATTGTTA TCAGGCCTGT CGACCTGAAT ATGCCGAAAG CGACACCACC GGCGTCGATT 
    CGTGGAGCTG TGCCGACGGC GATTACACAG GCTCTTCAAC TTCAACCAAA CGGCGGTGGA 
    GCAGTACTTC GTCAGGTTCG GCCAGCATCC GGGCTCCGCG AGCGACTTCA GGACGCTGAT 
    GTTCGGAATT TGGAGAAAAC GAACACAGCA CTGATGAGCT ATGAAGCAGT GAATGAGGAG 
    CTCGATCGAC TCGACGCTAA GCAGAATACG ATGACAATTG ACGAGCAACG GAGATATCGA 
    GAGCTACTGA ACGTTGTCGC CGAGCAGAGC CGAGCAAGGA AGCTGCTCTC GGAGATCAGA 
    TCACCACCTA CATCTTATCA AGAACGTGAA ATTCCGATTC ATGTGCAAAA AGAGCAGCAG 
    CAGCACCGGC GGGAAACTGT GATTGATGAT ATTCCACACT CGAGCTCAAG TGTTGAGAAT 
    GTGTCACCTG TGAGCCCGCC GGAAAGTCGA AAAGTTCAAT TTATTGATGA AGTTGTGCAG 
    TATCAGACGC CGACCAGCGG AGCCAATCAC GCTTTCTTTG ATGATGTGCC GGTTTTGGAT 
    TCGCCTGGGA TCGTCGGCAC CAACGAAATC TACCGTGATC CCCGCCAACG TCGGCTCAAC 
    GAGCTCCAAG ACCGCAATCG CAGCTCGGAA AATGGAGCGG CGGACGGCGC GAAGCTCGGC 
    TTCCGCGACA AGCAGCGCCT ATTCGCACGG CAAATCGGCG AGGACACGGC ACCACGCCAA 
    CGAATGAATG AGAGCAGTGC TCAGCGGCTC ATTGAGACGG ACATTGCCGC CACCCCGCAA 
    AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306402.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293331.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336163
    Clone ID Related Accession (Same CDS sequence) NM_001306402.1 , NM_001306402.2
    Accession Version NM_001306402.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCAGACC TCCGTGAAAT CGACGGACGG CCCATTCCAC CAACAGAGTG CCCGCTTTTC 
    GAGATGACTG CTCGAAGTGG AAACGGCGAG AATGGATTCG ATTCGTTTTT GGCGATCAAG 
    CGGAAACCGC ATGATTATCA AGTCCCCTCC ACCTCAGTTG TTGCTCCATC ATCAGTCACC 
    CTACCAATGT CTCCATTAAC ATCACTTCCA CCACAAATTC ACAATCTTCC AGTGCTTATT 
    GTGATGAATA ATGATGGTCA CTTGTCAACA CACCGAATAA CGTTACACGA GGGAGTAACA 
    GAGGTTGGAA GTGACAGTCA AATGTCCAAT TTCTCACAGC ATAATATTTA TCTCGATGGT 
    CCGGATATTC GTGGACGCCA CGCGGCAATC GCTTTCATGG AAGGCGTCGT CACACTAACA 
    CCATCCACTC GTGATGCCTA CCTAGAGGTG AACGGCCATC AACTGATGCA GACGGAGATC 
    CTCCGGGACG GTGATCACAT TCGCATCGGA GCCGATCATT CGTTCAAGTT TAGCACGTCG 
    TCGTCACAAG CAAATCAGAT TAACAGTACT AATATCAGCA ACTCGAACCA ACGACACTTC 
    CAACAACAGA CATTTTATCC GGAAATGGCT GATTCACGAG CTTCAACTGC CAGGTGTGCA 
    CCATCGGATT ACTGTAGTCT ACCACAACAG AACGATTATT ATCCGCCGAG CATGTCCGGC 
    TCATCAGTCT CATCATCACG GCAGGAGGAT CTTATGATGC ATTCCAATAA TAACATCCTG 
    CCACCGTACT CTACAAAATC CGCGCAGGAA CCCCTCCAAC TGCCGCTCTC AATTCAAATC 
    GGCGACTACA ATGTATGGGA TTTCCTGGCC GAAGTGATGT CGAATCGTGC GGCAACCTCA 
    TCGGAAATGT CCACATTCCG CCTATCGCCT GCCTACTCTC TCTACCTGGC ACTTCGATCA 
    TTCCACACAC ACAATAAACC GTACTCTGTG CAATTCTTCT CACGGATCGA GTCGAATTTC 
    AAGCAAATCG CTCAGGAATG CTCGTCGAAG GATGATCTGA TCTTTTGGCT TGCCAACGCA 
    TCGGAATTCA ATCATCTTGT CGAGCGGGAC ACTGAGCTGA AAGCGTCGAG ATATGGCAAA 
    ATATCTGTGG AAATTGAGAG AATATTCCGG CGATTGGTTG CAGTGCTTCA AAGTGTCATC 
    CGTCCGGCGG CGCGGGCTCT GTTGGACATT CATCTGAATG ATCACGACGC GACCGGCGAT 
    GTGATTCATC TTCTCGACAC CACGATGCGC CAGGCGAGAA TTTGTGGTCT CAACGCGGCG 
    CTCACAATTC AACTGTGCGG GCATCTCCTC CACATGGTCA ATGCCTTTGT GTTCAACAGC 
    TTGGTCTCAG TCAACCTTCC AGCTGCCCAG TTGAATACTC GCCTCGGAAA GTGTCTTCAG 
    TATCGGATAG AGTCGATTCA CAGATTCTGC GAACGAATGG GCGTCGAGAT GGCCGCCGAG 
    TGCCATTTGG ATAGATCCCG TCAGGCCGCT AACCTTCTCG CCACGCAGAA GAATGATGTT 
    GCCGCGTTGG GATCGACGTG CTATAAGCTG AACAGCCTCC AAGTGGTTCA TATCCTGTCG 
    GGATTCGAAC CAGACGACGG CGAGATTCCA TGTGACGATG ACGTCGTTGA TCGGATCTGT 
    AGACTCGCTG AAAAACAGGC GGATAAGCTG ACGTTGGAGG ATGGGCAGCG CGTGACACTG 
    GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC CTGATCCCTC AGGATGGTTA CTTTGTCGAG 
    CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG CAGTACTTGC AGCAACTTCA GCATAGGCGC 
    CTCTGTCATA CGGTGTCGCT TACACCGGCA CCGCCGGCTG CGATGACCCA TTCGATGCAT 
    GCGATGCATA TTGGAGCAAG CGATGCTCAT TATCATCAGC ATCAGCATCA GCAGCAGCAG 
    AACCCGATTG TGAGAAGTGT GTCGCAGACT ACACTGACCA GTATTCCGAC TACACAATTT 
    GGCGACAGTT TTGGAAGTGC CGTGTCGGAT ACGATATCGA AGGTGACACT GCGGAAGAAT 
    GGAGGAGGCA TCGGATTGAG TATTGTCGCT GCTCAGGGTG TTGGTGATCG TCAAATGGGA 
    ATTTATGTGA AGAAGGTTGT CGAAGGAACA CCGGCTGCAC ACGATGGACG ACTCGAGACT 
    GGTGATCAGT TATTGAGTGT TAACGGACAT TCGTTAATTG GAATTTCACA GGAAGACGCC 
    GCCCGTCTCA TGACCCAAAG TGGAAACGAG GTGCATTTCG ACGTCCGGAA AAGAGCCGCT 
    CACCGTAACG GGCTCAGCAA TTGGCTCGGA TCCCAAACAC CGACAACTTC AAATTTCATT 
    GCACCATCTC CATCTACAGT ACCCCCGCCG AGTTATTCAC ATGTTGGACG GACTCCGTCT 
    TATGATGGAC AACCACCACC GAGTTATAAT CACAGAAATG GTGGAGCCAG CGGAGCAGGA 
    GGATCATCTG GAAGAAATAG TGCTTTCGTA GCACCACTTT CGAATTCATC ATCAAATGCG 
    TCGAGTAGTC ATATGCAACA ACACTATGCT CATCATAATA TGATGCATAA TGGCAATTTG 
    AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT CCGAAACATT ATCGAAGCTC GTCGGCTAGT 
    GATTTGCAGC ACGATCCAAA CGCCTCATTC TCTCAATCAT CAATGACTGG AAGTGCGAGA 
    AGTTCAGTGT TTGACAAGCT TCCATCTCAC TACCGTCACT CGACACGGCC TACAGTAATC 
    CAGCCAACAC GTCCAAGTGC TCAATCTCCA TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG 
    CCGGTCAGCA ATCTGTACCG TCCGACGTCG GCGTCGAATC TGTTTGCACC GCCCAGAAAT 
    TCGGGCGGTC TCGTCGGATA TTCGAGTAAC GCGGCACGGG AGTCGACACA GGATATTCAT 
    CGAGCGGAGC CAGCCATTGT TATCAGGCCT GTCGACCTGA ATATGCCGAA AGCGACACCA 
    CCGGCGTCGA TTCGTGGAGC TGTGCCGACG GCGATTACAC AGGCTCTTCA ACTTCAACCA 
    AACGGCGGTG GAGCAGTACT TCGTCAGGTT CGGCCAGCAT CCGGGCTCCG CGAGCGACTT 
    CAGGACGCTG ATGTTCGGAA TTTGGAGAAA ACGAACACAG CACTGATGAG CTATGAAGCA 
    GTGAATGAGG AGCTCGATCG ACTCGACGCT AAGCAGAATA CGATGACAAT TGACGAGCAA 
    CGGAGATATC GAGAGCTACT GAACGTTGTC GCCGAGCAGA GCCGAGCAAG GAAGCTGCTC 
    TCGGAGATCA GATCACCACC TACATCTTAT CAAGAACGTG AAATTCCGAT TCATGTGCAA 
    AAAGAGCAGC AGCAGCACCG GCGGGAAACT GTGATTGATG ATATTCCACA CTCGAGCTCA 
    AGTGTTGAGA ATGTGTCACC TGTGAGCCCG CCGGAAAGTC GAAAAGTTCA ATTTATTGAT 
    GAAGTTGTGC AGTATCAGAC GCCGACCAGC GGAGCCAATC ACGCTTTCTT TGATGATGTG 
    CCGGTTTTGG ATTCGCCTGG GATCGTCGGC ACCAACGAAA TCTACCGTGA TCCCCGCCAA 
    CGTCGGCTCA ACGAGCTCCA AGACCGCAAT CGCAGCTCGG AAAATGGAGC GGCGGACGGC 
    GCGAAGCTCG GCTTCCGCGA CAAGCAGCGC CTATTCGCAC GGCAAATCGG CGAGGACACG 
    GCACCACGCC AACGAATGAA TGAGAGCAGT GCTCAGCGGC TCATTGAGAC GGACATTGCC 
    GCCACCCCGC AAAAGCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293331.1
    CDS1..3861
    Translation

    Target ORF information:

    RefSeq Version NM_001306402.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306402.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCAGACC TCCGTGAAAT CGACGGACGG CCCATTCCAC CAACAGAGTG CCCGCTTTTC 
    GAGATGACTG CTCGAAGTGG AAACGGCGAG AATGGATTCG ATTCGTTTTT GGCGATCAAG 
    CGGAAACCGC ATGATTATCA AGTCCCCTCC ACCTCAGTTG TTGCTCCATC ATCAGTCACC 
    CTACCAATGT CTCCATTAAC ATCACTTCCA CCACAAATTC ACAATCTTCC AGTGCTTATT 
    GTGATGAATA ATGATGGTCA CTTGTCAACA CACCGAATAA CGTTACACGA GGGAGTAACA 
    GAGGTTGGAA GTGACAGTCA AATGTCCAAT TTCTCACAGC ATAATATTTA TCTCGATGGT 
    CCGGATATTC GTGGACGCCA CGCGGCAATC GCTTTCATGG AAGGCGTCGT CACACTAACA 
    CCATCCACTC GTGATGCCTA CCTAGAGGTG AACGGCCATC AACTGATGCA GACGGAGATC 
    CTCCGGGACG GTGATCACAT TCGCATCGGA GCCGATCATT CGTTCAAGTT TAGCACGTCG 
    TCGTCACAAG CAAATCAGAT TAACAGTACT AATATCAGCA ACTCGAACCA ACGACACTTC 
    CAACAACAGA CATTTTATCC GGAAATGGCT GATTCACGAG CTTCAACTGC CAGGTGTGCA 
    CCATCGGATT ACTGTAGTCT ACCACAACAG AACGATTATT ATCCGCCGAG CATGTCCGGC 
    TCATCAGTCT CATCATCACG GCAGGAGGAT CTTATGATGC ATTCCAATAA TAACATCCTG 
    CCACCGTACT CTACAAAATC CGCGCAGGAA CCCCTCCAAC TGCCGCTCTC AATTCAAATC 
    GGCGACTACA ATGTATGGGA TTTCCTGGCC GAAGTGATGT CGAATCGTGC GGCAACCTCA 
    TCGGAAATGT CCACATTCCG CCTATCGCCT GCCTACTCTC TCTACCTGGC ACTTCGATCA 
    TTCCACACAC ACAATAAACC GTACTCTGTG CAATTCTTCT CACGGATCGA GTCGAATTTC 
    AAGCAAATCG CTCAGGAATG CTCGTCGAAG GATGATCTGA TCTTTTGGCT TGCCAACGCA 
    TCGGAATTCA ATCATCTTGT CGAGCGGGAC ACTGAGCTGA AAGCGTCGAG ATATGGCAAA 
    ATATCTGTGG AAATTGAGAG AATATTCCGG CGATTGGTTG CAGTGCTTCA AAGTGTCATC 
    CGTCCGGCGG CGCGGGCTCT GTTGGACATT CATCTGAATG ATCACGACGC GACCGGCGAT 
    GTGATTCATC TTCTCGACAC CACGATGCGC CAGGCGAGAA TTTGTGGTCT CAACGCGGCG 
    CTCACAATTC AACTGTGCGG GCATCTCCTC CACATGGTCA ATGCCTTTGT GTTCAACAGC 
    TTGGTCTCAG TCAACCTTCC AGCTGCCCAG TTGAATACTC GCCTCGGAAA GTGTCTTCAG 
    TATCGGATAG AGTCGATTCA CAGATTCTGC GAACGAATGG GCGTCGAGAT GGCCGCCGAG 
    TGCCATTTGG ATAGATCCCG TCAGGCCGCT AACCTTCTCG CCACGCAGAA GAATGATGTT 
    GCCGCGTTGG GATCGACGTG CTATAAGCTG AACAGCCTCC AAGTGGTTCA TATCCTGTCG 
    GGATTCGAAC CAGACGACGG CGAGATTCCA TGTGACGATG ACGTCGTTGA TCGGATCTGT 
    AGACTCGCTG AAAAACAGGC GGATAAGCTG ACGTTGGAGG ATGGGCAGCG CGTGACACTG 
    GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC CTGATCCCTC AGGATGGTTA CTTTGTCGAG 
    CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG CAGTACTTGC AGCAACTTCA GCATAGGCGC 
    CTCTGTCATA CGGTGTCGCT TACACCGGCA CCGCCGGCTG CGATGACCCA TTCGATGCAT 
    GCGATGCATA TTGGAGCAAG CGATGCTCAT TATCATCAGC ATCAGCATCA GCAGCAGCAG 
    AACCCGATTG TGAGAAGTGT GTCGCAGACT ACACTGACCA GTATTCCGAC TACACAATTT 
    GGCGACAGTT TTGGAAGTGC CGTGTCGGAT ACGATATCGA AGGTGACACT GCGGAAGAAT 
    GGAGGAGGCA TCGGATTGAG TATTGTCGCT GCTCAGGGTG TTGGTGATCG TCAAATGGGA 
    ATTTATGTGA AGAAGGTTGT CGAAGGAACA CCGGCTGCAC ACGATGGACG ACTCGAGACT 
    GGTGATCAGT TATTGAGTGT TAACGGACAT TCGTTAATTG GAATTTCACA GGAAGACGCC 
    GCCCGTCTCA TGACCCAAAG TGGAAACGAG GTGCATTTCG ACGTCCGGAA AAGAGCCGCT 
    CACCGTAACG GGCTCAGCAA TTGGCTCGGA TCCCAAACAC CGACAACTTC AAATTTCATT 
    GCACCATCTC CATCTACAGT ACCCCCGCCG AGTTATTCAC ATGTTGGACG GACTCCGTCT 
    TATGATGGAC AACCACCACC GAGTTATAAT CACAGAAATG GTGGAGCCAG CGGAGCAGGA 
    GGATCATCTG GAAGAAATAG TGCTTTCGTA GCACCACTTT CGAATTCATC ATCAAATGCG 
    TCGAGTAGTC ATATGCAACA ACACTATGCT CATCATAATA TGATGCATAA TGGCAATTTG 
    AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT CCGAAACATT ATCGAAGCTC GTCGGCTAGT 
    GATTTGCAGC ACGATCCAAA CGCCTCATTC TCTCAATCAT CAATGACTGG AAGTGCGAGA 
    AGTTCAGTGT TTGACAAGCT TCCATCTCAC TACCGTCACT CGACACGGCC TACAGTAATC 
    CAGCCAACAC GTCCAAGTGC TCAATCTCCA TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG 
    CCGGTCAGCA ATCTGTACCG TCCGACGTCG GCGTCGAATC TGTTTGCACC GCCCAGAAAT 
    TCGGGCGGTC TCGTCGGATA TTCGAGTAAC GCGGCACGGG AGTCGACACA GGATATTCAT 
    CGAGCGGAGC CAGCCATTGT TATCAGGCCT GTCGACCTGA ATATGCCGAA AGCGACACCA 
    CCGGCGTCGA TTCGTGGAGC TGTGCCGACG GCGATTACAC AGGCTCTTCA ACTTCAACCA 
    AACGGCGGTG GAGCAGTACT TCGTCAGGTT CGGCCAGCAT CCGGGCTCCG CGAGCGACTT 
    CAGGACGCTG ATGTTCGGAA TTTGGAGAAA ACGAACACAG CACTGATGAG CTATGAAGCA 
    GTGAATGAGG AGCTCGATCG ACTCGACGCT AAGCAGAATA CGATGACAAT TGACGAGCAA 
    CGGAGATATC GAGAGCTACT GAACGTTGTC GCCGAGCAGA GCCGAGCAAG GAAGCTGCTC 
    TCGGAGATCA GATCACCACC TACATCTTAT CAAGAACGTG AAATTCCGAT TCATGTGCAA 
    AAAGAGCAGC AGCAGCACCG GCGGGAAACT GTGATTGATG ATATTCCACA CTCGAGCTCA 
    AGTGTTGAGA ATGTGTCACC TGTGAGCCCG CCGGAAAGTC GAAAAGTTCA ATTTATTGAT 
    GAAGTTGTGC AGTATCAGAC GCCGACCAGC GGAGCCAATC ACGCTTTCTT TGATGATGTG 
    CCGGTTTTGG ATTCGCCTGG GATCGTCGGC ACCAACGAAA TCTACCGTGA TCCCCGCCAA 
    CGTCGGCTCA ACGAGCTCCA AGACCGCAAT CGCAGCTCGG AAAATGGAGC GGCGGACGGC 
    GCGAAGCTCG GCTTCCGCGA CAAGCAGCGC CTATTCGCAC GGCAAATCGG CGAGGACACG 
    GCACCACGCC AACGAATGAA TGAGAGCAGT GCTCAGCGGC TCATTGAGAC GGACATTGCC 
    GCCACCCCGC AAAAGCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306402.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293331.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336163
    Clone ID Related Accession (Same CDS sequence) NM_001306402.1 , NM_001306402.2
    Accession Version NM_001306402.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306402.1. COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCAGACC TCCGTGAAAT CGACGGACGG CCCATTCCAC CAACAGAGTG CCCGCTTTTC 
    GAGATGACTG CTCGAAGTGG AAACGGCGAG AATGGATTCG ATTCGTTTTT GGCGATCAAG 
    CGGAAACCGC ATGATTATCA AGTCCCCTCC ACCTCAGTTG TTGCTCCATC ATCAGTCACC 
    CTACCAATGT CTCCATTAAC ATCACTTCCA CCACAAATTC ACAATCTTCC AGTGCTTATT 
    GTGATGAATA ATGATGGTCA CTTGTCAACA CACCGAATAA CGTTACACGA GGGAGTAACA 
    GAGGTTGGAA GTGACAGTCA AATGTCCAAT TTCTCACAGC ATAATATTTA TCTCGATGGT 
    CCGGATATTC GTGGACGCCA CGCGGCAATC GCTTTCATGG AAGGCGTCGT CACACTAACA 
    CCATCCACTC GTGATGCCTA CCTAGAGGTG AACGGCCATC AACTGATGCA GACGGAGATC 
    CTCCGGGACG GTGATCACAT TCGCATCGGA GCCGATCATT CGTTCAAGTT TAGCACGTCG 
    TCGTCACAAG CAAATCAGAT TAACAGTACT AATATCAGCA ACTCGAACCA ACGACACTTC 
    CAACAACAGA CATTTTATCC GGAAATGGCT GATTCACGAG CTTCAACTGC CAGGTGTGCA 
    CCATCGGATT ACTGTAGTCT ACCACAACAG AACGATTATT ATCCGCCGAG CATGTCCGGC 
    TCATCAGTCT CATCATCACG GCAGGAGGAT CTTATGATGC ATTCCAATAA TAACATCCTG 
    CCACCGTACT CTACAAAATC CGCGCAGGAA CCCCTCCAAC TGCCGCTCTC AATTCAAATC 
    GGCGACTACA ATGTATGGGA TTTCCTGGCC GAAGTGATGT CGAATCGTGC GGCAACCTCA 
    TCGGAAATGT CCACATTCCG CCTATCGCCT GCCTACTCTC TCTACCTGGC ACTTCGATCA 
    TTCCACACAC ACAATAAACC GTACTCTGTG CAATTCTTCT CACGGATCGA GTCGAATTTC 
    AAGCAAATCG CTCAGGAATG CTCGTCGAAG GATGATCTGA TCTTTTGGCT TGCCAACGCA 
    TCGGAATTCA ATCATCTTGT CGAGCGGGAC ACTGAGCTGA AAGCGTCGAG ATATGGCAAA 
    ATATCTGTGG AAATTGAGAG AATATTCCGG CGATTGGTTG CAGTGCTTCA AAGTGTCATC 
    CGTCCGGCGG CGCGGGCTCT GTTGGACATT CATCTGAATG ATCACGACGC GACCGGCGAT 
    GTGATTCATC TTCTCGACAC CACGATGCGC CAGGCGAGAA TTTGTGGTCT CAACGCGGCG 
    CTCACAATTC AACTGTGCGG GCATCTCCTC CACATGGTCA ATGCCTTTGT GTTCAACAGC 
    TTGGTCTCAG TCAACCTTCC AGCTGCCCAG TTGAATACTC GCCTCGGAAA GTGTCTTCAG 
    TATCGGATAG AGTCGATTCA CAGATTCTGC GAACGAATGG GCGTCGAGAT GGCCGCCGAG 
    TGCCATTTGG ATAGATCCCG TCAGGCCGCT AACCTTCTCG CCACGCAGAA GAATGATGTT 
    GCCGCGTTGG GATCGACGTG CTATAAGCTG AACAGCCTCC AAGTGGTTCA TATCCTGTCG 
    GGATTCGAAC CAGACGACGG CGAGATTCCA TGTGACGATG ACGTCGTTGA TCGGATCTGT 
    AGACTCGCTG AAAAACAGGC GGATAAGCTG ACGTTGGAGG ATGGGCAGCG CGTGACACTG 
    GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC CTGATCCCTC AGGATGGTTA CTTTGTCGAG 
    CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG CAGTACTTGC AGCAACTTCA GCATAGGCGC 
    CTCTGTCATA CGGTGTCGCT TACACCGGCA CCGCCGGCTG CGATGACCCA TTCGATGCAT 
    GCGATGCATA TTGGAGCAAG CGATGCTCAT TATCATCAGC ATCAGCATCA GCAGCAGCAG 
    AACCCGATTG TGAGAAGTGT GTCGCAGACT ACACTGACCA GTATTCCGAC TACACAATTT 
    GGCGACAGTT TTGGAAGTGC CGTGTCGGAT ACGATATCGA AGGTGACACT GCGGAAGAAT 
    GGAGGAGGCA TCGGATTGAG TATTGTCGCT GCTCAGGGTG TTGGTGATCG TCAAATGGGA 
    ATTTATGTGA AGAAGGTTGT CGAAGGAACA CCGGCTGCAC ACGATGGACG ACTCGAGACT 
    GGTGATCAGT TATTGAGTGT TAACGGACAT TCGTTAATTG GAATTTCACA GGAAGACGCC 
    GCCCGTCTCA TGACCCAAAG TGGAAACGAG GTGCATTTCG ACGTCCGGAA AAGAGCCGCT 
    CACCGTAACG GGCTCAGCAA TTGGCTCGGA TCCCAAACAC CGACAACTTC AAATTTCATT 
    GCACCATCTC CATCTACAGT ACCCCCGCCG AGTTATTCAC ATGTTGGACG GACTCCGTCT 
    TATGATGGAC AACCACCACC GAGTTATAAT CACAGAAATG GTGGAGCCAG CGGAGCAGGA 
    GGATCATCTG GAAGAAATAG TGCTTTCGTA GCACCACTTT CGAATTCATC ATCAAATGCG 
    TCGAGTAGTC ATATGCAACA ACACTATGCT CATCATAATA TGATGCATAA TGGCAATTTG 
    AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT CCGAAACATT ATCGAAGCTC GTCGGCTAGT 
    GATTTGCAGC ACGATCCAAA CGCCTCATTC TCTCAATCAT CAATGACTGG AAGTGCGAGA 
    AGTTCAGTGT TTGACAAGCT TCCATCTCAC TACCGTCACT CGACACGGCC TACAGTAATC 
    CAGCCAACAC GTCCAAGTGC TCAATCTCCA TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG 
    CCGGTCAGCA ATCTGTACCG TCCGACGTCG GCGTCGAATC TGTTTGCACC GCCCAGAAAT 
    TCGGGCGGTC TCGTCGGATA TTCGAGTAAC GCGGCACGGG AGTCGACACA GGATATTCAT 
    CGAGCGGAGC CAGCCATTGT TATCAGGCCT GTCGACCTGA ATATGCCGAA AGCGACACCA 
    CCGGCGTCGA TTCGTGGAGC TGTGCCGACG GCGATTACAC AGGCTCTTCA ACTTCAACCA 
    AACGGCGGTG GAGCAGTACT TCGTCAGGTT CGGCCAGCAT CCGGGCTCCG CGAGCGACTT 
    CAGGACGCTG ATGTTCGGAA TTTGGAGAAA ACGAACACAG CACTGATGAG CTATGAAGCA 
    GTGAATGAGG AGCTCGATCG ACTCGACGCT AAGCAGAATA CGATGACAAT TGACGAGCAA 
    CGGAGATATC GAGAGCTACT GAACGTTGTC GCCGAGCAGA GCCGAGCAAG GAAGCTGCTC 
    TCGGAGATCA GATCACCACC TACATCTTAT CAAGAACGTG AAATTCCGAT TCATGTGCAA 
    AAAGAGCAGC AGCAGCACCG GCGGGAAACT GTGATTGATG ATATTCCACA CTCGAGCTCA 
    AGTGTTGAGA ATGTGTCACC TGTGAGCCCG CCGGAAAGTC GAAAAGTTCA ATTTATTGAT 
    GAAGTTGTGC AGTATCAGAC GCCGACCAGC GGAGCCAATC ACGCTTTCTT TGATGATGTG 
    CCGGTTTTGG ATTCGCCTGG GATCGTCGGC ACCAACGAAA TCTACCGTGA TCCCCGCCAA 
    CGTCGGCTCA ACGAGCTCCA AGACCGCAAT CGCAGCTCGG AAAATGGAGC GGCGGACGGC 
    GCGAAGCTCG GCTTCCGCGA CAAGCAGCGC CTATTCGCAC GGCAAATCGG CGAGGACACG 
    GCACCACGCC AACGAATGAA TGAGAGCAGT GCTCAGCGGC TCATTGAGAC GGACATTGCC 
    GCCACCCCGC AAAAGCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293331.1
    CDS1..3861
    Translation

    Target ORF information:

    RefSeq Version NM_001306402.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306402.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCAGACC TCCGTGAAAT CGACGGACGG CCCATTCCAC CAACAGAGTG CCCGCTTTTC 
    GAGATGACTG CTCGAAGTGG AAACGGCGAG AATGGATTCG ATTCGTTTTT GGCGATCAAG 
    CGGAAACCGC ATGATTATCA AGTCCCCTCC ACCTCAGTTG TTGCTCCATC ATCAGTCACC 
    CTACCAATGT CTCCATTAAC ATCACTTCCA CCACAAATTC ACAATCTTCC AGTGCTTATT 
    GTGATGAATA ATGATGGTCA CTTGTCAACA CACCGAATAA CGTTACACGA GGGAGTAACA 
    GAGGTTGGAA GTGACAGTCA AATGTCCAAT TTCTCACAGC ATAATATTTA TCTCGATGGT 
    CCGGATATTC GTGGACGCCA CGCGGCAATC GCTTTCATGG AAGGCGTCGT CACACTAACA 
    CCATCCACTC GTGATGCCTA CCTAGAGGTG AACGGCCATC AACTGATGCA GACGGAGATC 
    CTCCGGGACG GTGATCACAT TCGCATCGGA GCCGATCATT CGTTCAAGTT TAGCACGTCG 
    TCGTCACAAG CAAATCAGAT TAACAGTACT AATATCAGCA ACTCGAACCA ACGACACTTC 
    CAACAACAGA CATTTTATCC GGAAATGGCT GATTCACGAG CTTCAACTGC CAGGTGTGCA 
    CCATCGGATT ACTGTAGTCT ACCACAACAG AACGATTATT ATCCGCCGAG CATGTCCGGC 
    TCATCAGTCT CATCATCACG GCAGGAGGAT CTTATGATGC ATTCCAATAA TAACATCCTG 
    CCACCGTACT CTACAAAATC CGCGCAGGAA CCCCTCCAAC TGCCGCTCTC AATTCAAATC 
    GGCGACTACA ATGTATGGGA TTTCCTGGCC GAAGTGATGT CGAATCGTGC GGCAACCTCA 
    TCGGAAATGT CCACATTCCG CCTATCGCCT GCCTACTCTC TCTACCTGGC ACTTCGATCA 
    TTCCACACAC ACAATAAACC GTACTCTGTG CAATTCTTCT CACGGATCGA GTCGAATTTC 
    AAGCAAATCG CTCAGGAATG CTCGTCGAAG GATGATCTGA TCTTTTGGCT TGCCAACGCA 
    TCGGAATTCA ATCATCTTGT CGAGCGGGAC ACTGAGCTGA AAGCGTCGAG ATATGGCAAA 
    ATATCTGTGG AAATTGAGAG AATATTCCGG CGATTGGTTG CAGTGCTTCA AAGTGTCATC 
    CGTCCGGCGG CGCGGGCTCT GTTGGACATT CATCTGAATG ATCACGACGC GACCGGCGAT 
    GTGATTCATC TTCTCGACAC CACGATGCGC CAGGCGAGAA TTTGTGGTCT CAACGCGGCG 
    CTCACAATTC AACTGTGCGG GCATCTCCTC CACATGGTCA ATGCCTTTGT GTTCAACAGC 
    TTGGTCTCAG TCAACCTTCC AGCTGCCCAG TTGAATACTC GCCTCGGAAA GTGTCTTCAG 
    TATCGGATAG AGTCGATTCA CAGATTCTGC GAACGAATGG GCGTCGAGAT GGCCGCCGAG 
    TGCCATTTGG ATAGATCCCG TCAGGCCGCT AACCTTCTCG CCACGCAGAA GAATGATGTT 
    GCCGCGTTGG GATCGACGTG CTATAAGCTG AACAGCCTCC AAGTGGTTCA TATCCTGTCG 
    GGATTCGAAC CAGACGACGG CGAGATTCCA TGTGACGATG ACGTCGTTGA TCGGATCTGT 
    AGACTCGCTG AAAAACAGGC GGATAAGCTG ACGTTGGAGG ATGGGCAGCG CGTGACACTG 
    GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC CTGATCCCTC AGGATGGTTA CTTTGTCGAG 
    CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG CAGTACTTGC AGCAACTTCA GCATAGGCGC 
    CTCTGTCATA CGGTGTCGCT TACACCGGCA CCGCCGGCTG CGATGACCCA TTCGATGCAT 
    GCGATGCATA TTGGAGCAAG CGATGCTCAT TATCATCAGC ATCAGCATCA GCAGCAGCAG 
    AACCCGATTG TGAGAAGTGT GTCGCAGACT ACACTGACCA GTATTCCGAC TACACAATTT 
    GGCGACAGTT TTGGAAGTGC CGTGTCGGAT ACGATATCGA AGGTGACACT GCGGAAGAAT 
    GGAGGAGGCA TCGGATTGAG TATTGTCGCT GCTCAGGGTG TTGGTGATCG TCAAATGGGA 
    ATTTATGTGA AGAAGGTTGT CGAAGGAACA CCGGCTGCAC ACGATGGACG ACTCGAGACT 
    GGTGATCAGT TATTGAGTGT TAACGGACAT TCGTTAATTG GAATTTCACA GGAAGACGCC 
    GCCCGTCTCA TGACCCAAAG TGGAAACGAG GTGCATTTCG ACGTCCGGAA AAGAGCCGCT 
    CACCGTAACG GGCTCAGCAA TTGGCTCGGA TCCCAAACAC CGACAACTTC AAATTTCATT 
    GCACCATCTC CATCTACAGT ACCCCCGCCG AGTTATTCAC ATGTTGGACG GACTCCGTCT 
    TATGATGGAC AACCACCACC GAGTTATAAT CACAGAAATG GTGGAGCCAG CGGAGCAGGA 
    GGATCATCTG GAAGAAATAG TGCTTTCGTA GCACCACTTT CGAATTCATC ATCAAATGCG 
    TCGAGTAGTC ATATGCAACA ACACTATGCT CATCATAATA TGATGCATAA TGGCAATTTG 
    AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT CCGAAACATT ATCGAAGCTC GTCGGCTAGT 
    GATTTGCAGC ACGATCCAAA CGCCTCATTC TCTCAATCAT CAATGACTGG AAGTGCGAGA 
    AGTTCAGTGT TTGACAAGCT TCCATCTCAC TACCGTCACT CGACACGGCC TACAGTAATC 
    CAGCCAACAC GTCCAAGTGC TCAATCTCCA TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG 
    CCGGTCAGCA ATCTGTACCG TCCGACGTCG GCGTCGAATC TGTTTGCACC GCCCAGAAAT 
    TCGGGCGGTC TCGTCGGATA TTCGAGTAAC GCGGCACGGG AGTCGACACA GGATATTCAT 
    CGAGCGGAGC CAGCCATTGT TATCAGGCCT GTCGACCTGA ATATGCCGAA AGCGACACCA 
    CCGGCGTCGA TTCGTGGAGC TGTGCCGACG GCGATTACAC AGGCTCTTCA ACTTCAACCA 
    AACGGCGGTG GAGCAGTACT TCGTCAGGTT CGGCCAGCAT CCGGGCTCCG CGAGCGACTT 
    CAGGACGCTG ATGTTCGGAA TTTGGAGAAA ACGAACACAG CACTGATGAG CTATGAAGCA 
    GTGAATGAGG AGCTCGATCG ACTCGACGCT AAGCAGAATA CGATGACAAT TGACGAGCAA 
    CGGAGATATC GAGAGCTACT GAACGTTGTC GCCGAGCAGA GCCGAGCAAG GAAGCTGCTC 
    TCGGAGATCA GATCACCACC TACATCTTAT CAAGAACGTG AAATTCCGAT TCATGTGCAA 
    AAAGAGCAGC AGCAGCACCG GCGGGAAACT GTGATTGATG ATATTCCACA CTCGAGCTCA 
    AGTGTTGAGA ATGTGTCACC TGTGAGCCCG CCGGAAAGTC GAAAAGTTCA ATTTATTGAT 
    GAAGTTGTGC AGTATCAGAC GCCGACCAGC GGAGCCAATC ACGCTTTCTT TGATGATGTG 
    CCGGTTTTGG ATTCGCCTGG GATCGTCGGC ACCAACGAAA TCTACCGTGA TCCCCGCCAA 
    CGTCGGCTCA ACGAGCTCCA AGACCGCAAT CGCAGCTCGG AAAATGGAGC GGCGGACGGC 
    GCGAAGCTCG GCTTCCGCGA CAAGCAGCGC CTATTCGCAC GGCAAATCGG CGAGGACACG 
    GCACCACGCC AACGAATGAA TGAGAGCAGT GCTCAGCGGC TCATTGAGAC GGACATTGCC 
    GCCACCCCGC AAAAGCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306403.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293332.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336164
    Clone ID Related Accession (Same CDS sequence) NM_001306403.1 , NM_001306403.2
    Accession Version NM_001306403.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306403.1. COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    CTAAATGACC TGGAACTCAA CTCACCGCTC GCCAGATCCA CGCCGAATGA GGAGTACCGT 
    ATCCCGCCGC CTAGAGTACC TACAGTAACC TCCACCTCCT CCAACTATGC CCCTCTTCAT 
    TCTATTCGAC CGGCAAGCAT GTATGGATAT CAGAGCCCGA GCTCTTCTAC TACAGTAATC 
    CCGGCTCCAA AAGGAGTGGC TTCTTCCTCT AATAACACTT CTCCCCGAGT GAATGTGACG 
    TTGGGTGGAG CATTGAGCTC ATCGAGATCT CATAATAGTC TTTCACAGGA GCCAGCCATT 
    GTTATCAGGC CTGTCGACCT GAATATGCCG AAAGCGACAC CACCGGCGTC GATTCGTGGA 
    GCTGTGCCGA CGGCGATTAC ACAGGCTCTT CAACTTCAAC CAAACGGCGG TGGAGCAGTA 
    CTTCGTCAGG TTCGGCCAGC ATCCGGGCTC CGCGAGCGAC TTCAGGACGC TGATGTTCGG 
    AATTTGGAGA AAACGAACAC AGCACTGATG AGCTATGAAG CAGTGAATGA GGAGCTCGAT 
    CGACTCGACG CTAAGCAGAA TACGATGACA ATTGACGAGC AACGGAGATA TCGAGAGCTA 
    CTGAACGTTG TCGCCGAGCA GAGCCGAGCA AGGAAGCTGC TCTCGGAGAT CAGATCACCA 
    CCTACATCTT ATCAAGAACG TGAAATTCCG ATTCATGTGC AAAAAGAGCA GCAGCAGCAC 
    CGGCGGGAAA CTGTGATTGA TGATATTCCA CACTCGAGCT CAAGTGTTGA GAATGTGTCA 
    CCTGTGAGCC CGCCGGAAAG TCGAAAAGTT CAATTTATTG ATGAAGTTGT GCAGTATCAG 
    ACGCCGACCA GCGGAGCCAA TCACGCTTTC TTTGATGATG TGCCGGTTTT GGATTCGCCT 
    GGGATCGTCG GCACCAACGA AATCTACCGT GATCCCCGCC AACGTCGGCT CAACGAGCTC 
    CAAGACCGCA ATCGCAGCTC GGAAAATGGA GCGGCGGACG GCGCGAAGCT CGGCTTCCGC 
    GACAAGCAGC GCCTATTCGC ACGGCAAATC GGCGAGGACA CGGCACCACG CCAACGAATG 
    AATGAGAGCA GTGCTCAGCG GCTCATTGAG ACGGACATTG CCGCCACCCC GCAAAAGCCA 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293332.1
    CDS1..3963
    Translation

    Target ORF information:

    RefSeq Version NM_001306403.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306403.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    CTAAATGACC TGGAACTCAA CTCACCGCTC GCCAGATCCA CGCCGAATGA GGAGTACCGT 
    ATCCCGCCGC CTAGAGTACC TACAGTAACC TCCACCTCCT CCAACTATGC CCCTCTTCAT 
    TCTATTCGAC CGGCAAGCAT GTATGGATAT CAGAGCCCGA GCTCTTCTAC TACAGTAATC 
    CCGGCTCCAA AAGGAGTGGC TTCTTCCTCT AATAACACTT CTCCCCGAGT GAATGTGACG 
    TTGGGTGGAG CATTGAGCTC ATCGAGATCT CATAATAGTC TTTCACAGGA GCCAGCCATT 
    GTTATCAGGC CTGTCGACCT GAATATGCCG AAAGCGACAC CACCGGCGTC GATTCGTGGA 
    GCTGTGCCGA CGGCGATTAC ACAGGCTCTT CAACTTCAAC CAAACGGCGG TGGAGCAGTA 
    CTTCGTCAGG TTCGGCCAGC ATCCGGGCTC CGCGAGCGAC TTCAGGACGC TGATGTTCGG 
    AATTTGGAGA AAACGAACAC AGCACTGATG AGCTATGAAG CAGTGAATGA GGAGCTCGAT 
    CGACTCGACG CTAAGCAGAA TACGATGACA ATTGACGAGC AACGGAGATA TCGAGAGCTA 
    CTGAACGTTG TCGCCGAGCA GAGCCGAGCA AGGAAGCTGC TCTCGGAGAT CAGATCACCA 
    CCTACATCTT ATCAAGAACG TGAAATTCCG ATTCATGTGC AAAAAGAGCA GCAGCAGCAC 
    CGGCGGGAAA CTGTGATTGA TGATATTCCA CACTCGAGCT CAAGTGTTGA GAATGTGTCA 
    CCTGTGAGCC CGCCGGAAAG TCGAAAAGTT CAATTTATTG ATGAAGTTGT GCAGTATCAG 
    ACGCCGACCA GCGGAGCCAA TCACGCTTTC TTTGATGATG TGCCGGTTTT GGATTCGCCT 
    GGGATCGTCG GCACCAACGA AATCTACCGT GATCCCCGCC AACGTCGGCT CAACGAGCTC 
    CAAGACCGCA ATCGCAGCTC GGAAAATGGA GCGGCGGACG GCGCGAAGCT CGGCTTCCGC 
    GACAAGCAGC GCCTATTCGC ACGGCAAATC GGCGAGGACA CGGCACCACG CCAACGAATG 
    AATGAGAGCA GTGCTCAGCG GCTCATTGAG ACGGACATTG CCGCCACCCC GCAAAAGCCA 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306404.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293333.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336165
    Clone ID Related Accession (Same CDS sequence) NM_001306404.1 , NM_001306404.2
    Accession Version NM_001306404.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    GAGCCAGCCA TTGTTATCAG GCCTGTCGAC CTGAATATGC CGAAAGCGAC ACCACCGGCG 
    TCGATTCGTG GAGCTGTGCC GACGGCGATT ACACAGGCTC TTCAACTTCA ACCAAACGGC 
    GGTGGAGCAG TACTTCGTCA GGTTCGGCCA GCATCCGGGC TCCGCGAGCG ACTTCAGGAC 
    GCTGATGTTC GGAATTTGGA GAAAACGAAC ACAGCACTGA TGAGCTATGA AGCAGTGAAT 
    GAGGAGCTCG ATCGACTCGA CGCTAAGCAG AATACGATGA CAATTGACGA GCAACGGAGA 
    TATCGAGAGC TACTGAACGT TGTCGCCGAG CAGAGCCGAG CAAGGAAGCT GCTCTCGGAG 
    ATCAGATCAC CACCTACATC TTATCAAGAA CGTGAAATTC CGATTCATGT GCAAAAAGAG 
    CAGCAGCAGC ACCGGCGGGA AACTGTGATT GATGATATTC CACACTCGAG CTCAAGTGTT 
    GAGAATGTGT CACCTGTGAG CCCGCCGGAA AGTCGAAAAG TTCAATTTAT TGATGAAGTT 
    GTGCAGTATC AGACGCCGAC CAGCGGAGCC AATCACGCTT TCTTTGATGA TGTGCCGGTT 
    TTGGATTCGC CTGGGATCGT CGGCACCAAC GAAATCTACC GTGATCCCCG CCAACGTCGG 
    CTCAACGAGC TCCAAGACCG CAATCGCAGC TCGGAAAATG GAGCGGCGGA CGGCGCGAAG 
    CTCGGCTTCC GCGACAAGCA GCGCCTATTC GCACGGCAAA TCGGCGAGGA CACGGCACCA 
    CGCCAACGAA TGAATGAGAG CAGTGCTCAG CGGCTCATTG AGACGGACAT TGCCGCCACC 
    CCGCAAAAGC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293333.1
    CDS1..3675
    Translation

    Target ORF information:

    RefSeq Version NM_001306404.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306404.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    GAGCCAGCCA TTGTTATCAG GCCTGTCGAC CTGAATATGC CGAAAGCGAC ACCACCGGCG 
    TCGATTCGTG GAGCTGTGCC GACGGCGATT ACACAGGCTC TTCAACTTCA ACCAAACGGC 
    GGTGGAGCAG TACTTCGTCA GGTTCGGCCA GCATCCGGGC TCCGCGAGCG ACTTCAGGAC 
    GCTGATGTTC GGAATTTGGA GAAAACGAAC ACAGCACTGA TGAGCTATGA AGCAGTGAAT 
    GAGGAGCTCG ATCGACTCGA CGCTAAGCAG AATACGATGA CAATTGACGA GCAACGGAGA 
    TATCGAGAGC TACTGAACGT TGTCGCCGAG CAGAGCCGAG CAAGGAAGCT GCTCTCGGAG 
    ATCAGATCAC CACCTACATC TTATCAAGAA CGTGAAATTC CGATTCATGT GCAAAAAGAG 
    CAGCAGCAGC ACCGGCGGGA AACTGTGATT GATGATATTC CACACTCGAG CTCAAGTGTT 
    GAGAATGTGT CACCTGTGAG CCCGCCGGAA AGTCGAAAAG TTCAATTTAT TGATGAAGTT 
    GTGCAGTATC AGACGCCGAC CAGCGGAGCC AATCACGCTT TCTTTGATGA TGTGCCGGTT 
    TTGGATTCGC CTGGGATCGT CGGCACCAAC GAAATCTACC GTGATCCCCG CCAACGTCGG 
    CTCAACGAGC TCCAAGACCG CAATCGCAGC TCGGAAAATG GAGCGGCGGA CGGCGCGAAG 
    CTCGGCTTCC GCGACAAGCA GCGCCTATTC GCACGGCAAA TCGGCGAGGA CACGGCACCA 
    CGCCAACGAA TGAATGAGAG CAGTGCTCAG CGGCTCATTG AGACGGACAT TGCCGCCACC 
    CCGCAAAAGC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306404.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293333.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336165
    Clone ID Related Accession (Same CDS sequence) NM_001306404.1 , NM_001306404.2
    Accession Version NM_001306404.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306404.1. COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    GAGCCAGCCA TTGTTATCAG GCCTGTCGAC CTGAATATGC CGAAAGCGAC ACCACCGGCG 
    TCGATTCGTG GAGCTGTGCC GACGGCGATT ACACAGGCTC TTCAACTTCA ACCAAACGGC 
    GGTGGAGCAG TACTTCGTCA GGTTCGGCCA GCATCCGGGC TCCGCGAGCG ACTTCAGGAC 
    GCTGATGTTC GGAATTTGGA GAAAACGAAC ACAGCACTGA TGAGCTATGA AGCAGTGAAT 
    GAGGAGCTCG ATCGACTCGA CGCTAAGCAG AATACGATGA CAATTGACGA GCAACGGAGA 
    TATCGAGAGC TACTGAACGT TGTCGCCGAG CAGAGCCGAG CAAGGAAGCT GCTCTCGGAG 
    ATCAGATCAC CACCTACATC TTATCAAGAA CGTGAAATTC CGATTCATGT GCAAAAAGAG 
    CAGCAGCAGC ACCGGCGGGA AACTGTGATT GATGATATTC CACACTCGAG CTCAAGTGTT 
    GAGAATGTGT CACCTGTGAG CCCGCCGGAA AGTCGAAAAG TTCAATTTAT TGATGAAGTT 
    GTGCAGTATC AGACGCCGAC CAGCGGAGCC AATCACGCTT TCTTTGATGA TGTGCCGGTT 
    TTGGATTCGC CTGGGATCGT CGGCACCAAC GAAATCTACC GTGATCCCCG CCAACGTCGG 
    CTCAACGAGC TCCAAGACCG CAATCGCAGC TCGGAAAATG GAGCGGCGGA CGGCGCGAAG 
    CTCGGCTTCC GCGACAAGCA GCGCCTATTC GCACGGCAAA TCGGCGAGGA CACGGCACCA 
    CGCCAACGAA TGAATGAGAG CAGTGCTCAG CGGCTCATTG AGACGGACAT TGCCGCCACC 
    CCGCAAAAGC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293333.1
    CDS1..3675
    Translation

    Target ORF information:

    RefSeq Version NM_001306404.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306404.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    GAGCCAGCCA TTGTTATCAG GCCTGTCGAC CTGAATATGC CGAAAGCGAC ACCACCGGCG 
    TCGATTCGTG GAGCTGTGCC GACGGCGATT ACACAGGCTC TTCAACTTCA ACCAAACGGC 
    GGTGGAGCAG TACTTCGTCA GGTTCGGCCA GCATCCGGGC TCCGCGAGCG ACTTCAGGAC 
    GCTGATGTTC GGAATTTGGA GAAAACGAAC ACAGCACTGA TGAGCTATGA AGCAGTGAAT 
    GAGGAGCTCG ATCGACTCGA CGCTAAGCAG AATACGATGA CAATTGACGA GCAACGGAGA 
    TATCGAGAGC TACTGAACGT TGTCGCCGAG CAGAGCCGAG CAAGGAAGCT GCTCTCGGAG 
    ATCAGATCAC CACCTACATC TTATCAAGAA CGTGAAATTC CGATTCATGT GCAAAAAGAG 
    CAGCAGCAGC ACCGGCGGGA AACTGTGATT GATGATATTC CACACTCGAG CTCAAGTGTT 
    GAGAATGTGT CACCTGTGAG CCCGCCGGAA AGTCGAAAAG TTCAATTTAT TGATGAAGTT 
    GTGCAGTATC AGACGCCGAC CAGCGGAGCC AATCACGCTT TCTTTGATGA TGTGCCGGTT 
    TTGGATTCGC CTGGGATCGT CGGCACCAAC GAAATCTACC GTGATCCCCG CCAACGTCGG 
    CTCAACGAGC TCCAAGACCG CAATCGCAGC TCGGAAAATG GAGCGGCGGA CGGCGCGAAG 
    CTCGGCTTCC GCGACAAGCA GCGCCTATTC GCACGGCAAA TCGGCGAGGA CACGGCACCA 
    CGCCAACGAA TGAATGAGAG CAGTGCTCAG CGGCTCATTG AGACGGACAT TGCCGCCACC 
    CCGCAAAAGC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306405.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293334.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.
    Price & Availability↑
    CloneID OCa336166
    Clone ID Related Accession (Same CDS sequence) NM_001306405.2 , NM_001306405.1
    Accession Version NM_001306405.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3684bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306405.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGACGG AGATCCTCCG GGACGGTGAT CACATTCGCA TCGGAGCCGA TCATTCGTTC 
    AAGTTTAGCA CGTCGTCGTC ACAAGCAAAT CAGATTAACA GTACTAATAT CAGCAACTCG 
    AACCAACGAC ACTTCCAACA ACAGACATTT TATCCGGAAA TGGCTGATTC ACGAGCTTCA 
    ACTGCCAGGT GTGCACCATC GGATTACTGT AGTCTACCAC AACAGAACGA TTATTATCCG 
    CCGAGCATGT CCGGCTCATC AGTCTCATCA TCACGGCAGG AGGATCTTAT GATGCATTCC 
    AATAATAACA TCCTGCCACC GTACTCTACA AAATCCGCGC AGGAACCCCT CCAACTGCCG 
    CTCTCAATTC AAATCGGCGA CTACAATGTA TGGGATTTCC TGGCCGAAGT GATGTCGAAT 
    CGTGCGGCAA CCTCATCGGA AATGTCCACA TTCCGCCTAT CGCCTGCCTA CTCTCTCTAC 
    CTGGCACTTC GATCATTCCA CACACACAAT AAACCGTACT CTGTGCAATT CTTCTCACGG 
    ATCGAGTCGA ATTTCAAGCA AATCGCTCAG GAATGCTCGT CGAAGGATGA TCTGATCTTT 
    TGGCTTGCCA ACGCATCGGA ATTCAATCAT CTTGTCGAGC GGGACACTGA GCTGAAAGCG 
    TCGAGATATG GCAAAATATC TGTGGAAATT GAGAGAATAT TCCGGCGATT GGTTGCAGTG 
    CTTCAAAGTG TCATCCGTCC GGCGGCGCGG GCTCTGTTGG ACATTCATCT GAATGATCAC 
    GACGCGACCG GCGATGTGAT TCATCTTCTC GACACCACGA TGCGCCAGGC GAGAATTTGT 
    GGTCTCAACG CGGCGCTCAC AATTCAACTG TGCGGGCATC TCCTCCACAT GGTCAATGCC 
    TTTGTGTTCA ACAGCTTGGT CTCAGTCAAC CTTCCAGCTG CCCAGTTGAA TACTCGCCTC 
    GGAAAGTGTC TTCAGTATCG GATAGAGTCG ATTCACAGAT TCTGCGAACG AATGGGCGTC 
    GAGATGGCCG CCGAGTGCCA TTTGGATAGA TCCCGTCAGG CCGCTAACCT TCTCGCCACG 
    CAGAAGAATG ATGTTGCCGC GTTGGGATCG ACGTGCTATA AGCTGAACAG CCTCCAAGTG 
    GTTCATATCC TGTCGGGATT CGAACCAGAC GACGGCGAGA TTCCATGTGA CGATGACGTC 
    GTTGATCGGA TCTGTAGACT CGCTGAAAAA CAGGCGGATA AGCTGACGTT GGAGGATGGG 
    CAGCGCGTGA CACTGGAGGA GTCCGACGAG CTGAACCTTC CGTTCCTGAT CCCTCAGGAT 
    GGTTACTTTG TCGAGCAGTT CCGAACGGTG CCAGATGGTC TTTGGCAGTA CTTGCAGCAA 
    CTTCAGCATA GGCGCCTCTG TCATACGGTG TCGCTTACAC CGGCACCGCC GGCTGCGATG 
    ACCCATTCGA TGCATGCGAT GCATATTGGA GCAAGCGATG CTCATTATCA TCAGCATCAG 
    CATCAGCAGC AGCAGAACCC GATTGTGAGA AGTGTGTCGC AGACTACACT GACCAGTATT 
    CCGACTACAC AATTTGGCGA CAGTTTTGGA AGTGCCGTGT CGGATACGAT ATCGAAGGTG 
    ACACTGCGGA AGAATGGAGG AGGCATCGGA TTGAGTATTG TCGCTGCTCA GGGTGTTGGT 
    GATCGTCAAA TGGGAATTTA TGTGAAGAAG GTTGTCGAAG GAACACCGGC TGCACACGAT 
    GGACGACTCG AGACTGGTGA TCAGTTATTG AGTGTTAACG GACATTCGTT AATTGGAATT 
    TCACAGGAAG ACGCCGCCCG TCTCATGACC CAAAGTGGAA ACGAGGTGCA TTTCGACGTC 
    CGGAAAAGAG CCGCTCACCG TAACGGGCTC AGCAATTGGC TCGGATCCCA AACACCGACA 
    ACTTCAAATT TCATTGCACC ATCTCCATCT ACAGTACCCC CGCCGAGTTA TTCACATGTT 
    GGACGGACTC CGTCTTATGA TGGACAACCA CCACCGAGTT ATAATCACAG AAATGGTGGA 
    GCCAGCGGAG CAGGAGGATC ATCTGGAAGA AATAGTGCTT TCGTAGCACC ACTTTCGAAT 
    TCATCATCAA ATGCGTCGAG TAGTCATATG CAACAACACT ATGCTCATCA TAATATGATG 
    CATAATGGCA ATTTGAGTGC TGCTCCGGGA GCTCCCGCGG GGGATCCGAA ACATTATCGA 
    AGCTCGTCGG CTAGTGATTT GCAGCACGAT CCAAACGCCT CATTCTCTCA ATCATCAATG 
    ACTGGAAGTG CGAGAAGTTC AGTGTTTGAC AAGCTTCCAT CTCACTACCG TCACTCGACA 
    CGGCCTACAG TAATCCAGCC AACACGTCCA AGTGCTCAAT CTCCATCGGC TCTTCGAAAA 
    GCGGCCGCCG CCTCGCCGGT CAGCAATCTG TACCGTCCGA CGTCGGCGTC GAATCTGTTT 
    GCACCGCCCA GAAATTCGGG CGGTCTCGTC GGATATTCGA GTAACGCGGC ACGGGAGTCG 
    ACACAGGATA TTCATCGAGC GCTAAATGAC CTGGAACTCA ACTCACCGCT CGCCAGATCC 
    ACGCCGAATG AGGAGTACCG TATCCCGCCG CCTAGAGTAC CTACAGTAAC CTCCACCTCC 
    TCCAACTATG CCCCTCTTCA TTCTATTCGA CCGGCAAGCA TGTATGGATA TCAGAGCCCG 
    AGCTCTTCTA CTACAGTAAT CCCGGCTCCA AAAGGAGTGG CTTCTTCCTC TAATAACACT 
    TCTCCCCGAG TGAATGTGAC GTTGGGTGGA GCATTGAGCT CATCGAGATC TCATAATAGT 
    CTTTCACAGG AGCCAGCCAT TGTTATCAGG CCTGTCGACC TGAATATGCC GAAAGCGACA 
    CCACCGGCGT CGATTCGTGG AGCTGTGCCG ACGGCGATTA CACAGGCTCT TCAACTTCAA 
    CCAAACGGCG GTGGAGCAGT ACTTCGTCAG GTTCGGCCAG CATCCGGGCT CCGCGAGCGA 
    CTTCAGGACG CTGATGTTCG GAATTTGGAG AAAACGAACA CAGCACTGAT GAGCTATGAA 
    GCAGTGAATG AGGAGCTCGA TCGACTCGAC GCTAAGCAGA ATACGATGAC AATTGACGAG 
    CAACGGAGAT ATCGAGAGCT ACTGAACGTT GTCGCCGAGC AGAGCCGAGC AAGGAAGCTG 
    CTCTCGGAGA TCAGATCACC ACCTACATCT TATCAAGAAC GTGAAATTCC GATTCATGTG 
    CAAAAAGAGC AGCAGCAGCA CCGGCGGGAA ACTGTGATTG ATGATATTCC ACACTCGAGC 
    TCAAGTGTTG AGAATGTGTC ACCTGTGAGC CCGCCGGAAA GTCGAAAAGT TCAATTTATT 
    GATGAAGTTG TGCAGTATCA GACGCCGACC AGCGGAGCCA ATCACGCTTT CTTTGATGAT 
    GTGCCGGTTT TGGATTCGCC TGGGATCGTC GGCACCAACG AAATCTACCG TGATCCCCGC 
    CAACGTCGGC TCAACGAGCT CCAAGACCGC AATCGCAGCT CGGAAAATGG AGCGGCGGAC 
    GGCGCGAAGC TCGGCTTCCG CGACAAGCAG CGCCTATTCG CACGGCAAAT CGGCGAGGAC 
    ACGGCACCAC GCCAACGAAT GAATGAGAGC AGTGCTCAGC GGCTCATTGA GACGGACATT 
    GCCGCCACCC CGCAAAAGCC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293334.1
    CDS5..3688
    Translation

    Target ORF information:

    RefSeq Version NM_001306405.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306405.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGACGG AGATCCTCCG GGACGGTGAT CACATTCGCA TCGGAGCCGA TCATTCGTTC 
    AAGTTTAGCA CGTCGTCGTC ACAAGCAAAT CAGATTAACA GTACTAATAT CAGCAACTCG 
    AACCAACGAC ACTTCCAACA ACAGACATTT TATCCGGAAA TGGCTGATTC ACGAGCTTCA 
    ACTGCCAGGT GTGCACCATC GGATTACTGT AGTCTACCAC AACAGAACGA TTATTATCCG 
    CCGAGCATGT CCGGCTCATC AGTCTCATCA TCACGGCAGG AGGATCTTAT GATGCATTCC 
    AATAATAACA TCCTGCCACC GTACTCTACA AAATCCGCGC AGGAACCCCT CCAACTGCCG 
    CTCTCAATTC AAATCGGCGA CTACAATGTA TGGGATTTCC TGGCCGAAGT GATGTCGAAT 
    CGTGCGGCAA CCTCATCGGA AATGTCCACA TTCCGCCTAT CGCCTGCCTA CTCTCTCTAC 
    CTGGCACTTC GATCATTCCA CACACACAAT AAACCGTACT CTGTGCAATT CTTCTCACGG 
    ATCGAGTCGA ATTTCAAGCA AATCGCTCAG GAATGCTCGT CGAAGGATGA TCTGATCTTT 
    TGGCTTGCCA ACGCATCGGA ATTCAATCAT CTTGTCGAGC GGGACACTGA GCTGAAAGCG 
    TCGAGATATG GCAAAATATC TGTGGAAATT GAGAGAATAT TCCGGCGATT GGTTGCAGTG 
    CTTCAAAGTG TCATCCGTCC GGCGGCGCGG GCTCTGTTGG ACATTCATCT GAATGATCAC 
    GACGCGACCG GCGATGTGAT TCATCTTCTC GACACCACGA TGCGCCAGGC GAGAATTTGT 
    GGTCTCAACG CGGCGCTCAC AATTCAACTG TGCGGGCATC TCCTCCACAT GGTCAATGCC 
    TTTGTGTTCA ACAGCTTGGT CTCAGTCAAC CTTCCAGCTG CCCAGTTGAA TACTCGCCTC 
    GGAAAGTGTC TTCAGTATCG GATAGAGTCG ATTCACAGAT TCTGCGAACG AATGGGCGTC 
    GAGATGGCCG CCGAGTGCCA TTTGGATAGA TCCCGTCAGG CCGCTAACCT TCTCGCCACG 
    CAGAAGAATG ATGTTGCCGC GTTGGGATCG ACGTGCTATA AGCTGAACAG CCTCCAAGTG 
    GTTCATATCC TGTCGGGATT CGAACCAGAC GACGGCGAGA TTCCATGTGA CGATGACGTC 
    GTTGATCGGA TCTGTAGACT CGCTGAAAAA CAGGCGGATA AGCTGACGTT GGAGGATGGG 
    CAGCGCGTGA CACTGGAGGA GTCCGACGAG CTGAACCTTC CGTTCCTGAT CCCTCAGGAT 
    GGTTACTTTG TCGAGCAGTT CCGAACGGTG CCAGATGGTC TTTGGCAGTA CTTGCAGCAA 
    CTTCAGCATA GGCGCCTCTG TCATACGGTG TCGCTTACAC CGGCACCGCC GGCTGCGATG 
    ACCCATTCGA TGCATGCGAT GCATATTGGA GCAAGCGATG CTCATTATCA TCAGCATCAG 
    CATCAGCAGC AGCAGAACCC GATTGTGAGA AGTGTGTCGC AGACTACACT GACCAGTATT 
    CCGACTACAC AATTTGGCGA CAGTTTTGGA AGTGCCGTGT CGGATACGAT ATCGAAGGTG 
    ACACTGCGGA AGAATGGAGG AGGCATCGGA TTGAGTATTG TCGCTGCTCA GGGTGTTGGT 
    GATCGTCAAA TGGGAATTTA TGTGAAGAAG GTTGTCGAAG GAACACCGGC TGCACACGAT 
    GGACGACTCG AGACTGGTGA TCAGTTATTG AGTGTTAACG GACATTCGTT AATTGGAATT 
    TCACAGGAAG ACGCCGCCCG TCTCATGACC CAAAGTGGAA ACGAGGTGCA TTTCGACGTC 
    CGGAAAAGAG CCGCTCACCG TAACGGGCTC AGCAATTGGC TCGGATCCCA AACACCGACA 
    ACTTCAAATT TCATTGCACC ATCTCCATCT ACAGTACCCC CGCCGAGTTA TTCACATGTT 
    GGACGGACTC CGTCTTATGA TGGACAACCA CCACCGAGTT ATAATCACAG AAATGGTGGA 
    GCCAGCGGAG CAGGAGGATC ATCTGGAAGA AATAGTGCTT TCGTAGCACC ACTTTCGAAT 
    TCATCATCAA ATGCGTCGAG TAGTCATATG CAACAACACT ATGCTCATCA TAATATGATG 
    CATAATGGCA ATTTGAGTGC TGCTCCGGGA GCTCCCGCGG GGGATCCGAA ACATTATCGA 
    AGCTCGTCGG CTAGTGATTT GCAGCACGAT CCAAACGCCT CATTCTCTCA ATCATCAATG 
    ACTGGAAGTG CGAGAAGTTC AGTGTTTGAC AAGCTTCCAT CTCACTACCG TCACTCGACA 
    CGGCCTACAG TAATCCAGCC AACACGTCCA AGTGCTCAAT CTCCATCGGC TCTTCGAAAA 
    GCGGCCGCCG CCTCGCCGGT CAGCAATCTG TACCGTCCGA CGTCGGCGTC GAATCTGTTT 
    GCACCGCCCA GAAATTCGGG CGGTCTCGTC GGATATTCGA GTAACGCGGC ACGGGAGTCG 
    ACACAGGATA TTCATCGAGC GCTAAATGAC CTGGAACTCA ACTCACCGCT CGCCAGATCC 
    ACGCCGAATG AGGAGTACCG TATCCCGCCG CCTAGAGTAC CTACAGTAAC CTCCACCTCC 
    TCCAACTATG CCCCTCTTCA TTCTATTCGA CCGGCAAGCA TGTATGGATA TCAGAGCCCG 
    AGCTCTTCTA CTACAGTAAT CCCGGCTCCA AAAGGAGTGG CTTCTTCCTC TAATAACACT 
    TCTCCCCGAG TGAATGTGAC GTTGGGTGGA GCATTGAGCT CATCGAGATC TCATAATAGT 
    CTTTCACAGG AGCCAGCCAT TGTTATCAGG CCTGTCGACC TGAATATGCC GAAAGCGACA 
    CCACCGGCGT CGATTCGTGG AGCTGTGCCG ACGGCGATTA CACAGGCTCT TCAACTTCAA 
    CCAAACGGCG GTGGAGCAGT ACTTCGTCAG GTTCGGCCAG CATCCGGGCT CCGCGAGCGA 
    CTTCAGGACG CTGATGTTCG GAATTTGGAG AAAACGAACA CAGCACTGAT GAGCTATGAA 
    GCAGTGAATG AGGAGCTCGA TCGACTCGAC GCTAAGCAGA ATACGATGAC AATTGACGAG 
    CAACGGAGAT ATCGAGAGCT ACTGAACGTT GTCGCCGAGC AGAGCCGAGC AAGGAAGCTG 
    CTCTCGGAGA TCAGATCACC ACCTACATCT TATCAAGAAC GTGAAATTCC GATTCATGTG 
    CAAAAAGAGC AGCAGCAGCA CCGGCGGGAA ACTGTGATTG ATGATATTCC ACACTCGAGC 
    TCAAGTGTTG AGAATGTGTC ACCTGTGAGC CCGCCGGAAA GTCGAAAAGT TCAATTTATT 
    GATGAAGTTG TGCAGTATCA GACGCCGACC AGCGGAGCCA ATCACGCTTT CTTTGATGAT 
    GTGCCGGTTT TGGATTCGCC TGGGATCGTC GGCACCAACG AAATCTACCG TGATCCCCGC 
    CAACGTCGGC TCAACGAGCT CCAAGACCGC AATCGCAGCT CGGAAAATGG AGCGGCGGAC 
    GGCGCGAAGC TCGGCTTCCG CGACAAGCAG CGCCTATTCG CACGGCAAAT CGGCGAGGAC 
    ACGGCACCAC GCCAACGAAT GAATGAGAGC AGTGCTCAGC GGCTCATTGA GACGGACATT 
    GCCGCCACCC CGCAAAAGCC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306406.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293335.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336167
    Clone ID Related Accession (Same CDS sequence) NM_001306406.1 , NM_001306406.2
    Accession Version NM_001306406.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGACGG AGATCCTCCG GGACGGTGAT CACATTCGCA TCGGAGCCGA TCATTCGTTC 
    AAGTTTAGCA CGTCGTCGTC ACAAGCAAAT CAGATTAACA GTACTAATAT CAGCAACTCG 
    AACCAACGAC ACTTCCAACA ACAGACATTT TATCCGGAAA TGGCTGATTC ACGAGCTTCA 
    ACTGCCAGGT GTGCACCATC GGATTACTGT AGTCTACCAC AACAGAACGA TTATTATCCG 
    CCGAGCATGT CCGGCTCATC AGTCTCATCA TCACGGCAGG AGGATCTTAT GATGCATTCC 
    AATAATAACA TCCTGCCACC GTACTCTACA AAATCCGCGC AGGAACCCCT CCAACTGCCG 
    CTCTCAATTC AAATCGGCGA CTACAATGTA TGGGATTTCC TGGCCGAAGT GATGTCGAAT 
    CGTGCGGCAA CCTCATCGGA AATGTCCACA TTCCGCCTAT CGCCTGCCTA CTCTCTCTAC 
    CTGGCACTTC GATCATTCCA CACACACAAT AAACCGTACT CTGTGCAATT CTTCTCACGG 
    ATCGAGTCGA ATTTCAAGCA AATCGCTCAG GAATGCTCGT CGAAGGATGA TCTGATCTTT 
    TGGCTTGCCA ACGCATCGGA ATTCAATCAT CTTGTCGAGC GGGACACTGA GCTGAAAGCG 
    TCGAGATATG GCAAAATATC TGTGGAAATT GAGAGAATAT TCCGGCGATT GGTTGCAGTG 
    CTTCAAAGTG TCATCCGTCC GGCGGCGCGG GCTCTGTTGG ACATTCATCT GAATGATCAC 
    GACGCGACCG GCGATGTGAT TCATCTTCTC GACACCACGA TGCGCCAGGC GAGAATTTGT 
    GGTCTCAACG CGGCGCTCAC AATTCAACTG TGCGGGCATC TCCTCCACAT GGTCAATGCC 
    TTTGTGTTCA ACAGCTTGGT CTCAGTCAAC CTTCCAGCTG CCCAGTTGAA TACTCGCCTC 
    GGAAAGTGTC TTCAGTATCG GATAGAGTCG ATTCACAGAT TCTGCGAACG AATGGGCGTC 
    GAGATGGCCG CCGAGTGCCA TTTGGATAGA TCCCGTCAGG CCGCTAACCT TCTCGCCACG 
    CAGAAGAATG ATGTTGCCGC GTTGGGATCG ACGTGCTATA AGCTGAACAG CCTCCAAGTG 
    GTTCATATCC TGTCGGGATT CGAACCAGAC GACGGCGAGA TTCCATGTGA CGATGACGTC 
    GTTGATCGGA TCTGTAGACT CGCTGAAAAA CAGGCGGATA AGCTGACGTT GGAGGATGGG 
    CAGCGCGTGA CACTGGAGGA GTCCGACGAG CTGAACCTTC CGTTCCTGAT CCCTCAGGAT 
    GGTTACTTTG TCGAGCAGTT CCGAACGGTG CCAGATGGTC TTTGGCAGTA CTTGCAGCAA 
    CTTCAGCATA GGCGCCTCTG TCATACGGTG TCGCTTACAC CGGCACCGCC GGCTGCGATG 
    ACCCATTCGA TGCATGCGAT GCATATTGGA GCAAGCGATG CTCATTATCA TCAGCATCAG 
    CATCAGCAGC AGCAGAACCC GATTGTGAGA AGTGTGTCGC AGACTACACT GACCAGTATT 
    CCGACTACAC AATTTGGCGA CAGTTTTGGA AGTGCCGTGT CGGATACGAT ATCGAAGGTG 
    ACACTGCGGA AGAATGGAGG AGGCATCGGA TTGAGTATTG TCGCTGCTCA GGGTGTTGGT 
    GATCGTCAAA TGGGAATTTA TGTGAAGAAG GTTGTCGAAG GAACACCGGC TGCACACGAT 
    GGACGACTCG AGACTGGTGA TCAGTTATTG AGTGTTAACG GACATTCGTT AATTGGAATT 
    TCACAGGAAG ACGCCGCCCG TCTCATGACC CAAAGTGGAA ACGAGGTGCA TTTCGACGTC 
    CGGAAAAGAG CCGCTCACCG TAACGGGCTC AGCAATTGGC TCGGATCCCA AACACCGACA 
    ACTTCAAATT TCATTGCACC ATCTCCATCT ACAGTACCCC CGCCGAGTTA TTCACATGTT 
    GGACGGACTC CGTCTTATGA TGGACAACCA CCACCGAGTT ATAATCACAG AAATGGTGGA 
    GCCAGCGGAG CAGGAGGATC ATCTGGAAGA AATAGTGCTT TCGTAGCACC ACTTTCGAAT 
    TCATCATCAA ATGCGTCGAG TAGTCATATG CAACAACACT ATGCTCATCA TAATATGATG 
    CATAATGGCA ATTTGAGTGC TGCTCCGGGA GCTCCCGCGG GGGATCCGAA ACATTATCGA 
    AGCTCGTCGG CTAGTGATTT GCAGCACGAT CCAAACGCCT CATTCTCTCA ATCATCAATG 
    ACTGGAAGTG CGAGAAGTTC AGTGTTTGAC AAGCTTCCAT CTCACTACCG TCACTCGACA 
    CGGCCTACAG TAATCCAGCC AACACGTCCA AGTGCTCAAT CTCCATCGGC TCTTCGAAAA 
    GCGGCCGCCG CCTCGCCGGT CAGCAATCTG TACCGTCCGA CGTCGGCGTC GAATCTGTTT 
    GCACCGCCCA GAAATTCGGG CGGTCTCGTC GGATATTCGA GTAACGCGGC ACGGGAGTCG 
    ACACAGGATA TTCATCGAGC GGAGCCAGCC ATTGTTATCA GGCCTGTCGA CCTGAATATG 
    CCGAAAGCGA CACCACCGGC GTCGATTCGT GGAGCTGTGC CGACGGCGAT TACACAGGCT 
    CTTCAACTTC AACCAAACGG CGGTGGAGCA GTACTTCGTC AGGTTCGGCC AGCATCCGGG 
    CTCCGCGAGC GACTTCAGGA CGCTGATGTT CGGAATTTGG AGAAAACGAA CACAGCACTG 
    ATGAGCTATG AAGCAGTGAA TGAGGAGCTC GATCGACTCG ACGCTAAGCA GAATACGATG 
    ACAATTGACG AGCAACGGAG ATATCGAGAG CTACTGAACG TTGTCGCCGA GCAGAGCCGA 
    GCAAGGAAGC TGCTCTCGGA GATCAGATCA CCACCTACAT CTTATCAAGA ACGTGAAATT 
    CCGATTCATG TGCAAAAAGA GCAGCAGCAG CACCGGCGGG AAACTGTGAT TGATGATATT 
    CCACACTCGA GCTCAAGTGT TGAGAATGTG TCACCTGTGA GCCCGCCGGA AAGTCGAAAA 
    GTTCAATTTA TTGATGAAGT TGTGCAGTAT CAGACGCCGA CCAGCGGAGC CAATCACGCT 
    TTCTTTGATG ATGTGCCGGT TTTGGATTCG CCTGGGATCG TCGGCACCAA CGAAATCTAC 
    CGTGATCCCC GCCAACGTCG GCTCAACGAG CTCCAAGACC GCAATCGCAG CTCGGAAAAT 
    GGAGCGGCGG ACGGCGCGAA GCTCGGCTTC CGCGACAAGC AGCGCCTATT CGCACGGCAA 
    ATCGGCGAGG ACACGGCACC ACGCCAACGA ATGAATGAGA GCAGTGCTCA GCGGCTCATT 
    GAGACGGACA TTGCCGCCAC CCCGCAAAAG CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293335.1
    CDS1..3396
    Translation

    Target ORF information:

    RefSeq Version NM_001306406.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306406.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGACGG AGATCCTCCG GGACGGTGAT CACATTCGCA TCGGAGCCGA TCATTCGTTC 
    AAGTTTAGCA CGTCGTCGTC ACAAGCAAAT CAGATTAACA GTACTAATAT CAGCAACTCG 
    AACCAACGAC ACTTCCAACA ACAGACATTT TATCCGGAAA TGGCTGATTC ACGAGCTTCA 
    ACTGCCAGGT GTGCACCATC GGATTACTGT AGTCTACCAC AACAGAACGA TTATTATCCG 
    CCGAGCATGT CCGGCTCATC AGTCTCATCA TCACGGCAGG AGGATCTTAT GATGCATTCC 
    AATAATAACA TCCTGCCACC GTACTCTACA AAATCCGCGC AGGAACCCCT CCAACTGCCG 
    CTCTCAATTC AAATCGGCGA CTACAATGTA TGGGATTTCC TGGCCGAAGT GATGTCGAAT 
    CGTGCGGCAA CCTCATCGGA AATGTCCACA TTCCGCCTAT CGCCTGCCTA CTCTCTCTAC 
    CTGGCACTTC GATCATTCCA CACACACAAT AAACCGTACT CTGTGCAATT CTTCTCACGG 
    ATCGAGTCGA ATTTCAAGCA AATCGCTCAG GAATGCTCGT CGAAGGATGA TCTGATCTTT 
    TGGCTTGCCA ACGCATCGGA ATTCAATCAT CTTGTCGAGC GGGACACTGA GCTGAAAGCG 
    TCGAGATATG GCAAAATATC TGTGGAAATT GAGAGAATAT TCCGGCGATT GGTTGCAGTG 
    CTTCAAAGTG TCATCCGTCC GGCGGCGCGG GCTCTGTTGG ACATTCATCT GAATGATCAC 
    GACGCGACCG GCGATGTGAT TCATCTTCTC GACACCACGA TGCGCCAGGC GAGAATTTGT 
    GGTCTCAACG CGGCGCTCAC AATTCAACTG TGCGGGCATC TCCTCCACAT GGTCAATGCC 
    TTTGTGTTCA ACAGCTTGGT CTCAGTCAAC CTTCCAGCTG CCCAGTTGAA TACTCGCCTC 
    GGAAAGTGTC TTCAGTATCG GATAGAGTCG ATTCACAGAT TCTGCGAACG AATGGGCGTC 
    GAGATGGCCG CCGAGTGCCA TTTGGATAGA TCCCGTCAGG CCGCTAACCT TCTCGCCACG 
    CAGAAGAATG ATGTTGCCGC GTTGGGATCG ACGTGCTATA AGCTGAACAG CCTCCAAGTG 
    GTTCATATCC TGTCGGGATT CGAACCAGAC GACGGCGAGA TTCCATGTGA CGATGACGTC 
    GTTGATCGGA TCTGTAGACT CGCTGAAAAA CAGGCGGATA AGCTGACGTT GGAGGATGGG 
    CAGCGCGTGA CACTGGAGGA GTCCGACGAG CTGAACCTTC CGTTCCTGAT CCCTCAGGAT 
    GGTTACTTTG TCGAGCAGTT CCGAACGGTG CCAGATGGTC TTTGGCAGTA CTTGCAGCAA 
    CTTCAGCATA GGCGCCTCTG TCATACGGTG TCGCTTACAC CGGCACCGCC GGCTGCGATG 
    ACCCATTCGA TGCATGCGAT GCATATTGGA GCAAGCGATG CTCATTATCA TCAGCATCAG 
    CATCAGCAGC AGCAGAACCC GATTGTGAGA AGTGTGTCGC AGACTACACT GACCAGTATT 
    CCGACTACAC AATTTGGCGA CAGTTTTGGA AGTGCCGTGT CGGATACGAT ATCGAAGGTG 
    ACACTGCGGA AGAATGGAGG AGGCATCGGA TTGAGTATTG TCGCTGCTCA GGGTGTTGGT 
    GATCGTCAAA TGGGAATTTA TGTGAAGAAG GTTGTCGAAG GAACACCGGC TGCACACGAT 
    GGACGACTCG AGACTGGTGA TCAGTTATTG AGTGTTAACG GACATTCGTT AATTGGAATT 
    TCACAGGAAG ACGCCGCCCG TCTCATGACC CAAAGTGGAA ACGAGGTGCA TTTCGACGTC 
    CGGAAAAGAG CCGCTCACCG TAACGGGCTC AGCAATTGGC TCGGATCCCA AACACCGACA 
    ACTTCAAATT TCATTGCACC ATCTCCATCT ACAGTACCCC CGCCGAGTTA TTCACATGTT 
    GGACGGACTC CGTCTTATGA TGGACAACCA CCACCGAGTT ATAATCACAG AAATGGTGGA 
    GCCAGCGGAG CAGGAGGATC ATCTGGAAGA AATAGTGCTT TCGTAGCACC ACTTTCGAAT 
    TCATCATCAA ATGCGTCGAG TAGTCATATG CAACAACACT ATGCTCATCA TAATATGATG 
    CATAATGGCA ATTTGAGTGC TGCTCCGGGA GCTCCCGCGG GGGATCCGAA ACATTATCGA 
    AGCTCGTCGG CTAGTGATTT GCAGCACGAT CCAAACGCCT CATTCTCTCA ATCATCAATG 
    ACTGGAAGTG CGAGAAGTTC AGTGTTTGAC AAGCTTCCAT CTCACTACCG TCACTCGACA 
    CGGCCTACAG TAATCCAGCC AACACGTCCA AGTGCTCAAT CTCCATCGGC TCTTCGAAAA 
    GCGGCCGCCG CCTCGCCGGT CAGCAATCTG TACCGTCCGA CGTCGGCGTC GAATCTGTTT 
    GCACCGCCCA GAAATTCGGG CGGTCTCGTC GGATATTCGA GTAACGCGGC ACGGGAGTCG 
    ACACAGGATA TTCATCGAGC GGAGCCAGCC ATTGTTATCA GGCCTGTCGA CCTGAATATG 
    CCGAAAGCGA CACCACCGGC GTCGATTCGT GGAGCTGTGC CGACGGCGAT TACACAGGCT 
    CTTCAACTTC AACCAAACGG CGGTGGAGCA GTACTTCGTC AGGTTCGGCC AGCATCCGGG 
    CTCCGCGAGC GACTTCAGGA CGCTGATGTT CGGAATTTGG AGAAAACGAA CACAGCACTG 
    ATGAGCTATG AAGCAGTGAA TGAGGAGCTC GATCGACTCG ACGCTAAGCA GAATACGATG 
    ACAATTGACG AGCAACGGAG ATATCGAGAG CTACTGAACG TTGTCGCCGA GCAGAGCCGA 
    GCAAGGAAGC TGCTCTCGGA GATCAGATCA CCACCTACAT CTTATCAAGA ACGTGAAATT 
    CCGATTCATG TGCAAAAAGA GCAGCAGCAG CACCGGCGGG AAACTGTGAT TGATGATATT 
    CCACACTCGA GCTCAAGTGT TGAGAATGTG TCACCTGTGA GCCCGCCGGA AAGTCGAAAA 
    GTTCAATTTA TTGATGAAGT TGTGCAGTAT CAGACGCCGA CCAGCGGAGC CAATCACGCT 
    TTCTTTGATG ATGTGCCGGT TTTGGATTCG CCTGGGATCG TCGGCACCAA CGAAATCTAC 
    CGTGATCCCC GCCAACGTCG GCTCAACGAG CTCCAAGACC GCAATCGCAG CTCGGAAAAT 
    GGAGCGGCGG ACGGCGCGAA GCTCGGCTTC CGCGACAAGC AGCGCCTATT CGCACGGCAA 
    ATCGGCGAGG ACACGGCACC ACGCCAACGA ATGAATGAGA GCAGTGCTCA GCGGCTCATT 
    GAGACGGACA TTGCCGCCAC CCCGCAAAAG CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306406.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293335.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336167
    Clone ID Related Accession (Same CDS sequence) NM_001306406.1 , NM_001306406.2
    Accession Version NM_001306406.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306406.1. COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGACGG AGATCCTCCG GGACGGTGAT CACATTCGCA TCGGAGCCGA TCATTCGTTC 
    AAGTTTAGCA CGTCGTCGTC ACAAGCAAAT CAGATTAACA GTACTAATAT CAGCAACTCG 
    AACCAACGAC ACTTCCAACA ACAGACATTT TATCCGGAAA TGGCTGATTC ACGAGCTTCA 
    ACTGCCAGGT GTGCACCATC GGATTACTGT AGTCTACCAC AACAGAACGA TTATTATCCG 
    CCGAGCATGT CCGGCTCATC AGTCTCATCA TCACGGCAGG AGGATCTTAT GATGCATTCC 
    AATAATAACA TCCTGCCACC GTACTCTACA AAATCCGCGC AGGAACCCCT CCAACTGCCG 
    CTCTCAATTC AAATCGGCGA CTACAATGTA TGGGATTTCC TGGCCGAAGT GATGTCGAAT 
    CGTGCGGCAA CCTCATCGGA AATGTCCACA TTCCGCCTAT CGCCTGCCTA CTCTCTCTAC 
    CTGGCACTTC GATCATTCCA CACACACAAT AAACCGTACT CTGTGCAATT CTTCTCACGG 
    ATCGAGTCGA ATTTCAAGCA AATCGCTCAG GAATGCTCGT CGAAGGATGA TCTGATCTTT 
    TGGCTTGCCA ACGCATCGGA ATTCAATCAT CTTGTCGAGC GGGACACTGA GCTGAAAGCG 
    TCGAGATATG GCAAAATATC TGTGGAAATT GAGAGAATAT TCCGGCGATT GGTTGCAGTG 
    CTTCAAAGTG TCATCCGTCC GGCGGCGCGG GCTCTGTTGG ACATTCATCT GAATGATCAC 
    GACGCGACCG GCGATGTGAT TCATCTTCTC GACACCACGA TGCGCCAGGC GAGAATTTGT 
    GGTCTCAACG CGGCGCTCAC AATTCAACTG TGCGGGCATC TCCTCCACAT GGTCAATGCC 
    TTTGTGTTCA ACAGCTTGGT CTCAGTCAAC CTTCCAGCTG CCCAGTTGAA TACTCGCCTC 
    GGAAAGTGTC TTCAGTATCG GATAGAGTCG ATTCACAGAT TCTGCGAACG AATGGGCGTC 
    GAGATGGCCG CCGAGTGCCA TTTGGATAGA TCCCGTCAGG CCGCTAACCT TCTCGCCACG 
    CAGAAGAATG ATGTTGCCGC GTTGGGATCG ACGTGCTATA AGCTGAACAG CCTCCAAGTG 
    GTTCATATCC TGTCGGGATT CGAACCAGAC GACGGCGAGA TTCCATGTGA CGATGACGTC 
    GTTGATCGGA TCTGTAGACT CGCTGAAAAA CAGGCGGATA AGCTGACGTT GGAGGATGGG 
    CAGCGCGTGA CACTGGAGGA GTCCGACGAG CTGAACCTTC CGTTCCTGAT CCCTCAGGAT 
    GGTTACTTTG TCGAGCAGTT CCGAACGGTG CCAGATGGTC TTTGGCAGTA CTTGCAGCAA 
    CTTCAGCATA GGCGCCTCTG TCATACGGTG TCGCTTACAC CGGCACCGCC GGCTGCGATG 
    ACCCATTCGA TGCATGCGAT GCATATTGGA GCAAGCGATG CTCATTATCA TCAGCATCAG 
    CATCAGCAGC AGCAGAACCC GATTGTGAGA AGTGTGTCGC AGACTACACT GACCAGTATT 
    CCGACTACAC AATTTGGCGA CAGTTTTGGA AGTGCCGTGT CGGATACGAT ATCGAAGGTG 
    ACACTGCGGA AGAATGGAGG AGGCATCGGA TTGAGTATTG TCGCTGCTCA GGGTGTTGGT 
    GATCGTCAAA TGGGAATTTA TGTGAAGAAG GTTGTCGAAG GAACACCGGC TGCACACGAT 
    GGACGACTCG AGACTGGTGA TCAGTTATTG AGTGTTAACG GACATTCGTT AATTGGAATT 
    TCACAGGAAG ACGCCGCCCG TCTCATGACC CAAAGTGGAA ACGAGGTGCA TTTCGACGTC 
    CGGAAAAGAG CCGCTCACCG TAACGGGCTC AGCAATTGGC TCGGATCCCA AACACCGACA 
    ACTTCAAATT TCATTGCACC ATCTCCATCT ACAGTACCCC CGCCGAGTTA TTCACATGTT 
    GGACGGACTC CGTCTTATGA TGGACAACCA CCACCGAGTT ATAATCACAG AAATGGTGGA 
    GCCAGCGGAG CAGGAGGATC ATCTGGAAGA AATAGTGCTT TCGTAGCACC ACTTTCGAAT 
    TCATCATCAA ATGCGTCGAG TAGTCATATG CAACAACACT ATGCTCATCA TAATATGATG 
    CATAATGGCA ATTTGAGTGC TGCTCCGGGA GCTCCCGCGG GGGATCCGAA ACATTATCGA 
    AGCTCGTCGG CTAGTGATTT GCAGCACGAT CCAAACGCCT CATTCTCTCA ATCATCAATG 
    ACTGGAAGTG CGAGAAGTTC AGTGTTTGAC AAGCTTCCAT CTCACTACCG TCACTCGACA 
    CGGCCTACAG TAATCCAGCC AACACGTCCA AGTGCTCAAT CTCCATCGGC TCTTCGAAAA 
    GCGGCCGCCG CCTCGCCGGT CAGCAATCTG TACCGTCCGA CGTCGGCGTC GAATCTGTTT 
    GCACCGCCCA GAAATTCGGG CGGTCTCGTC GGATATTCGA GTAACGCGGC ACGGGAGTCG 
    ACACAGGATA TTCATCGAGC GGAGCCAGCC ATTGTTATCA GGCCTGTCGA CCTGAATATG 
    CCGAAAGCGA CACCACCGGC GTCGATTCGT GGAGCTGTGC CGACGGCGAT TACACAGGCT 
    CTTCAACTTC AACCAAACGG CGGTGGAGCA GTACTTCGTC AGGTTCGGCC AGCATCCGGG 
    CTCCGCGAGC GACTTCAGGA CGCTGATGTT CGGAATTTGG AGAAAACGAA CACAGCACTG 
    ATGAGCTATG AAGCAGTGAA TGAGGAGCTC GATCGACTCG ACGCTAAGCA GAATACGATG 
    ACAATTGACG AGCAACGGAG ATATCGAGAG CTACTGAACG TTGTCGCCGA GCAGAGCCGA 
    GCAAGGAAGC TGCTCTCGGA GATCAGATCA CCACCTACAT CTTATCAAGA ACGTGAAATT 
    CCGATTCATG TGCAAAAAGA GCAGCAGCAG CACCGGCGGG AAACTGTGAT TGATGATATT 
    CCACACTCGA GCTCAAGTGT TGAGAATGTG TCACCTGTGA GCCCGCCGGA AAGTCGAAAA 
    GTTCAATTTA TTGATGAAGT TGTGCAGTAT CAGACGCCGA CCAGCGGAGC CAATCACGCT 
    TTCTTTGATG ATGTGCCGGT TTTGGATTCG CCTGGGATCG TCGGCACCAA CGAAATCTAC 
    CGTGATCCCC GCCAACGTCG GCTCAACGAG CTCCAAGACC GCAATCGCAG CTCGGAAAAT 
    GGAGCGGCGG ACGGCGCGAA GCTCGGCTTC CGCGACAAGC AGCGCCTATT CGCACGGCAA 
    ATCGGCGAGG ACACGGCACC ACGCCAACGA ATGAATGAGA GCAGTGCTCA GCGGCTCATT 
    GAGACGGACA TTGCCGCCAC CCCGCAAAAG CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293335.1
    CDS1..3396
    Translation

    Target ORF information:

    RefSeq Version NM_001306406.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306406.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGACGG AGATCCTCCG GGACGGTGAT CACATTCGCA TCGGAGCCGA TCATTCGTTC 
    AAGTTTAGCA CGTCGTCGTC ACAAGCAAAT CAGATTAACA GTACTAATAT CAGCAACTCG 
    AACCAACGAC ACTTCCAACA ACAGACATTT TATCCGGAAA TGGCTGATTC ACGAGCTTCA 
    ACTGCCAGGT GTGCACCATC GGATTACTGT AGTCTACCAC AACAGAACGA TTATTATCCG 
    CCGAGCATGT CCGGCTCATC AGTCTCATCA TCACGGCAGG AGGATCTTAT GATGCATTCC 
    AATAATAACA TCCTGCCACC GTACTCTACA AAATCCGCGC AGGAACCCCT CCAACTGCCG 
    CTCTCAATTC AAATCGGCGA CTACAATGTA TGGGATTTCC TGGCCGAAGT GATGTCGAAT 
    CGTGCGGCAA CCTCATCGGA AATGTCCACA TTCCGCCTAT CGCCTGCCTA CTCTCTCTAC 
    CTGGCACTTC GATCATTCCA CACACACAAT AAACCGTACT CTGTGCAATT CTTCTCACGG 
    ATCGAGTCGA ATTTCAAGCA AATCGCTCAG GAATGCTCGT CGAAGGATGA TCTGATCTTT 
    TGGCTTGCCA ACGCATCGGA ATTCAATCAT CTTGTCGAGC GGGACACTGA GCTGAAAGCG 
    TCGAGATATG GCAAAATATC TGTGGAAATT GAGAGAATAT TCCGGCGATT GGTTGCAGTG 
    CTTCAAAGTG TCATCCGTCC GGCGGCGCGG GCTCTGTTGG ACATTCATCT GAATGATCAC 
    GACGCGACCG GCGATGTGAT TCATCTTCTC GACACCACGA TGCGCCAGGC GAGAATTTGT 
    GGTCTCAACG CGGCGCTCAC AATTCAACTG TGCGGGCATC TCCTCCACAT GGTCAATGCC 
    TTTGTGTTCA ACAGCTTGGT CTCAGTCAAC CTTCCAGCTG CCCAGTTGAA TACTCGCCTC 
    GGAAAGTGTC TTCAGTATCG GATAGAGTCG ATTCACAGAT TCTGCGAACG AATGGGCGTC 
    GAGATGGCCG CCGAGTGCCA TTTGGATAGA TCCCGTCAGG CCGCTAACCT TCTCGCCACG 
    CAGAAGAATG ATGTTGCCGC GTTGGGATCG ACGTGCTATA AGCTGAACAG CCTCCAAGTG 
    GTTCATATCC TGTCGGGATT CGAACCAGAC GACGGCGAGA TTCCATGTGA CGATGACGTC 
    GTTGATCGGA TCTGTAGACT CGCTGAAAAA CAGGCGGATA AGCTGACGTT GGAGGATGGG 
    CAGCGCGTGA CACTGGAGGA GTCCGACGAG CTGAACCTTC CGTTCCTGAT CCCTCAGGAT 
    GGTTACTTTG TCGAGCAGTT CCGAACGGTG CCAGATGGTC TTTGGCAGTA CTTGCAGCAA 
    CTTCAGCATA GGCGCCTCTG TCATACGGTG TCGCTTACAC CGGCACCGCC GGCTGCGATG 
    ACCCATTCGA TGCATGCGAT GCATATTGGA GCAAGCGATG CTCATTATCA TCAGCATCAG 
    CATCAGCAGC AGCAGAACCC GATTGTGAGA AGTGTGTCGC AGACTACACT GACCAGTATT 
    CCGACTACAC AATTTGGCGA CAGTTTTGGA AGTGCCGTGT CGGATACGAT ATCGAAGGTG 
    ACACTGCGGA AGAATGGAGG AGGCATCGGA TTGAGTATTG TCGCTGCTCA GGGTGTTGGT 
    GATCGTCAAA TGGGAATTTA TGTGAAGAAG GTTGTCGAAG GAACACCGGC TGCACACGAT 
    GGACGACTCG AGACTGGTGA TCAGTTATTG AGTGTTAACG GACATTCGTT AATTGGAATT 
    TCACAGGAAG ACGCCGCCCG TCTCATGACC CAAAGTGGAA ACGAGGTGCA TTTCGACGTC 
    CGGAAAAGAG CCGCTCACCG TAACGGGCTC AGCAATTGGC TCGGATCCCA AACACCGACA 
    ACTTCAAATT TCATTGCACC ATCTCCATCT ACAGTACCCC CGCCGAGTTA TTCACATGTT 
    GGACGGACTC CGTCTTATGA TGGACAACCA CCACCGAGTT ATAATCACAG AAATGGTGGA 
    GCCAGCGGAG CAGGAGGATC ATCTGGAAGA AATAGTGCTT TCGTAGCACC ACTTTCGAAT 
    TCATCATCAA ATGCGTCGAG TAGTCATATG CAACAACACT ATGCTCATCA TAATATGATG 
    CATAATGGCA ATTTGAGTGC TGCTCCGGGA GCTCCCGCGG GGGATCCGAA ACATTATCGA 
    AGCTCGTCGG CTAGTGATTT GCAGCACGAT CCAAACGCCT CATTCTCTCA ATCATCAATG 
    ACTGGAAGTG CGAGAAGTTC AGTGTTTGAC AAGCTTCCAT CTCACTACCG TCACTCGACA 
    CGGCCTACAG TAATCCAGCC AACACGTCCA AGTGCTCAAT CTCCATCGGC TCTTCGAAAA 
    GCGGCCGCCG CCTCGCCGGT CAGCAATCTG TACCGTCCGA CGTCGGCGTC GAATCTGTTT 
    GCACCGCCCA GAAATTCGGG CGGTCTCGTC GGATATTCGA GTAACGCGGC ACGGGAGTCG 
    ACACAGGATA TTCATCGAGC GGAGCCAGCC ATTGTTATCA GGCCTGTCGA CCTGAATATG 
    CCGAAAGCGA CACCACCGGC GTCGATTCGT GGAGCTGTGC CGACGGCGAT TACACAGGCT 
    CTTCAACTTC AACCAAACGG CGGTGGAGCA GTACTTCGTC AGGTTCGGCC AGCATCCGGG 
    CTCCGCGAGC GACTTCAGGA CGCTGATGTT CGGAATTTGG AGAAAACGAA CACAGCACTG 
    ATGAGCTATG AAGCAGTGAA TGAGGAGCTC GATCGACTCG ACGCTAAGCA GAATACGATG 
    ACAATTGACG AGCAACGGAG ATATCGAGAG CTACTGAACG TTGTCGCCGA GCAGAGCCGA 
    GCAAGGAAGC TGCTCTCGGA GATCAGATCA CCACCTACAT CTTATCAAGA ACGTGAAATT 
    CCGATTCATG TGCAAAAAGA GCAGCAGCAG CACCGGCGGG AAACTGTGAT TGATGATATT 
    CCACACTCGA GCTCAAGTGT TGAGAATGTG TCACCTGTGA GCCCGCCGGA AAGTCGAAAA 
    GTTCAATTTA TTGATGAAGT TGTGCAGTAT CAGACGCCGA CCAGCGGAGC CAATCACGCT 
    TTCTTTGATG ATGTGCCGGT TTTGGATTCG CCTGGGATCG TCGGCACCAA CGAAATCTAC 
    CGTGATCCCC GCCAACGTCG GCTCAACGAG CTCCAAGACC GCAATCGCAG CTCGGAAAAT 
    GGAGCGGCGG ACGGCGCGAA GCTCGGCTTC CGCGACAAGC AGCGCCTATT CGCACGGCAA 
    ATCGGCGAGG ACACGGCACC ACGCCAACGA ATGAATGAGA GCAGTGCTCA GCGGCTCATT 
    GAGACGGACA TTGCCGCCAC CCCGCAAAAG CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306407.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293336.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.
    Price & Availability↑
    CloneID OCa336168
    Clone ID Related Accession (Same CDS sequence) NM_001306407.1 , NM_001306407.2
    Accession Version NM_001306407.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306407.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCCGGCT CATCAGTCTC ATCATCACGG CAGGAGGATC TTATGATGCA TTCCAATAAT 
    AACATCCTGC CACCGTACTC TACAAAATCC GCGCAGGAAC CCCTCCAACT GCCGCTCTCA 
    ATTCAAATCG GCGACTACAA TGTATGGGAT TTCCTGGCCG AAGTGATGTC GAATCGTGCG 
    GCAACCTCAT CGGAAATGTC CACATTCCGC CTATCGCCTG CCTACTCTCT CTACCTGGCA 
    CTTCGATCAT TCCACACACA CAATAAACCG TACTCTGTGC AATTCTTCTC ACGGATCGAG 
    TCGAATTTCA AGCAAATCGC TCAGGAATGC TCGTCGAAGG ATGATCTGAT CTTTTGGCTT 
    GCCAACGCAT CGGAATTCAA TCATCTTGTC GAGCGGGACA CTGAGCTGAA AGCGTCGAGA 
    TATGGCAAAA TATCTGTGGA AATTGAGAGA ATATTCCGGC GATTGGTTGC AGTGCTTCAA 
    AGTGTCATCC GTCCGGCGGC GCGGGCTCTG TTGGACATTC ATCTGAATGA TCACGACGCG 
    ACCGGCGATG TGATTCATCT TCTCGACACC ACGATGCGCC AGGCGAGAAT TTGTGGTCTC 
    AACGCGGCGC TCACAATTCA ACTGTGCGGG CATCTCCTCC ACATGGTCAA TGCCTTTGTG 
    TTCAACAGCT TGGTCTCAGT CAACCTTCCA GCTGCCCAGT TGAATACTCG CCTCGGAAAG 
    TGTCTTCAGT ATCGGATAGA GTCGATTCAC AGATTCTGCG AACGAATGGG CGTCGAGATG 
    GCCGCCGAGT GCCATTTGGA TAGATCCCGT CAGGCCGCTA ACCTTCTCGC CACGCAGAAG 
    AATGATGTTG CCGCGTTGGG ATCGACGTGC TATAAGCTGA ACAGCCTCCA AGTGGTTCAT 
    ATCCTGTCGG GATTCGAACC AGACGACGGC GAGATTCCAT GTGACGATGA CGTCGTTGAT 
    CGGATCTGTA GACTCGCTGA AAAACAGGCG GATAAGCTGA CGTTGGAGGA TGGGCAGCGC 
    GTGACACTGG AGGAGTCCGA CGAGCTGAAC CTTCCGTTCC TGATCCCTCA GGATGGTTAC 
    TTTGTCGAGC AGTTCCGAAC GGTGCCAGAT GGTCTTTGGC AGTACTTGCA GCAACTTCAG 
    CATAGGCGCC TCTGTCATAC GGTGTCGCTT ACACCGGCAC CGCCGGCTGC GATGACCCAT 
    TCGATGCATG CGATGCATAT TGGAGCAAGC GATGCTCATT ATCATCAGCA TCAGCATCAG 
    CAGCAGCAGA ACCCGATTGT GAGAAGTGTG TCGCAGACTA CACTGACCAG TATTCCGACT 
    ACACAATTTG GCGACAGTTT TGGAAGTGCC GTGTCGGATA CGATATCGAA GGTGACACTG 
    CGGAAGAATG GAGGAGGCAT CGGATTGAGT ATTGTCGCTG CTCAGGGTGT TGGTGATCGT 
    CAAATGGGAA TTTATGTGAA GAAGGTTGTC GAAGGAACAC CGGCTGCACA CGATGGACGA 
    CTCGAGACTG GTGATCAGTT ATTGAGTGTT AACGGACATT CGTTAATTGG AATTTCACAG 
    GAAGACGCCG CCCGTCTCAT GACCCAAAGT GGAAACGAGG TGCATTTCGA CGTCCGGAAA 
    AGAGCCGCTC ACCGTAACGG GCTCAGCAAT TGGCTCGGAT CCCAAACACC GACAACTTCA 
    AATTTCATTG CACCATCTCC ATCTACAGTA CCCCCGCCGA GTTATTCACA TGTTGGACGG 
    ACTCCGTCTT ATGATGGACA ACCACCACCG AGTTATAATC ACAGAAATGG TGGAGCCAGC 
    GGAGCAGGAG GATCATCTGG AAGAAATAGT GCTTTCGTAG CACCACTTTC GAATTCATCA 
    TCAAATGCGT CGAGTAGTCA TATGCAACAA CACTATGCTC ATCATAATAT GATGCATAAT 
    GGCAATTTGA GTGCTGCTCC GGGAGCTCCC GCGGGGGATC CGAAACATTA TCGAAGCTCG 
    TCGGCTAGTG ATTTGCAGCA CGATCCAAAC GCCTCATTCT CTCAATCATC AATGACTGGA 
    AGTGCGAGAA GTTCAGTGTT TGACAAGCTT CCATCTCACT ACCGTCACTC GACACGGCCT 
    ACAGTAATCC AGCCAACACG TCCAAGTGCT CAATCTCCAT CGGCTCTTCG AAAAGCGGCC 
    GCCGCCTCGC CGGTCAGCAA TCTGTACCGT CCGACGTCGG CGTCGAATCT GTTTGCACCG 
    CCCAGAAATT CGGGCGGTCT CGTCGGATAT TCGAGTAACG CGGCACGGGA GTCGACACAG 
    GATATTCATC GAGCGCTAAA TGACCTGGAA CTCAACTCAC CGCTCGCCAG ATCCACGCCG 
    AATGAGGAGT ACCGTATCCC GCCGCCTAGA GTACCTACAG TAACCTCCAC CTCCTCCAAC 
    TATGCCCCTC TTCATTCTAT TCGACCGGCA AGCATGTATG GATATCAGAG CCCGAGCTCT 
    TCTACTACAG TAATCCCGGC TCCAAAAGGA GTGGCTTCTT CCTCTAATAA CACTTCTCCC 
    CGAGTGAATG TGACGTTGGG TGGAGCATTG AGCTCATCGA GATCTCATAA TAGTCTTTCA 
    CAGGAGCCAG CCATTGTTAT CAGGCCTGTC GACCTGAATA TGCCGAAAGC GACACCACCG 
    GCGTCGATTC GTGGAGCTGT GCCGACGGCG ATTACACAGG CTCTTCAACT TCAACCAAAC 
    GGCGGTGGAG CAGTACTTCG TCAGGTTCGG CCAGCATCCG GGCTCCGCGA GCGACTTCAG 
    GACGCTGATG TTCGGAATTT GGAGAAAACG AACACAGCAC TGATGAGCTA TGAAGCAGTG 
    AATGAGGAGC TCGATCGACT CGACGCTAAG CAGAATACGA TGACAATTGA CGAGCAACGG 
    AGATATCGAG AGCTACTGAA CGTTGTCGCC GAGCAGAGCC GAGCAAGGAA GCTGCTCTCG 
    GAGATCAGAT CACCACCTAC ATCTTATCAA GAACGTGAAA TTCCGATTCA TGTGCAAAAA 
    GAGCAGCAGC AGCACCGGCG GGAAACTGTG ATTGATGATA TTCCACACTC GAGCTCAAGT 
    GTTGAGAATG TGTCACCTGT GAGCCCGCCG GAAAGTCGAA AAGTTCAATT TATTGATGAA 
    GTTGTGCAGT ATCAGACGCC GACCAGCGGA GCCAATCACG CTTTCTTTGA TGATGTGCCG 
    GTTTTGGATT CGCCTGGGAT CGTCGGCACC AACGAAATCT ACCGTGATCC CCGCCAACGT 
    CGGCTCAACG AGCTCCAAGA CCGCAATCGC AGCTCGGAAA ATGGAGCGGC GGACGGCGCG 
    AAGCTCGGCT TCCGCGACAA GCAGCGCCTA TTCGCACGGC AAATCGGCGA GGACACGGCA 
    CCACGCCAAC GAATGAATGA GAGCAGTGCT CAGCGGCTCA TTGAGACGGA CATTGCCGCC 
    ACCCCGCAAA AGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293336.1
    CDS2..3439
    Translation

    Target ORF information:

    RefSeq Version NM_001306407.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306407.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCCGGCT CATCAGTCTC ATCATCACGG CAGGAGGATC TTATGATGCA TTCCAATAAT 
    AACATCCTGC CACCGTACTC TACAAAATCC GCGCAGGAAC CCCTCCAACT GCCGCTCTCA 
    ATTCAAATCG GCGACTACAA TGTATGGGAT TTCCTGGCCG AAGTGATGTC GAATCGTGCG 
    GCAACCTCAT CGGAAATGTC CACATTCCGC CTATCGCCTG CCTACTCTCT CTACCTGGCA 
    CTTCGATCAT TCCACACACA CAATAAACCG TACTCTGTGC AATTCTTCTC ACGGATCGAG 
    TCGAATTTCA AGCAAATCGC TCAGGAATGC TCGTCGAAGG ATGATCTGAT CTTTTGGCTT 
    GCCAACGCAT CGGAATTCAA TCATCTTGTC GAGCGGGACA CTGAGCTGAA AGCGTCGAGA 
    TATGGCAAAA TATCTGTGGA AATTGAGAGA ATATTCCGGC GATTGGTTGC AGTGCTTCAA 
    AGTGTCATCC GTCCGGCGGC GCGGGCTCTG TTGGACATTC ATCTGAATGA TCACGACGCG 
    ACCGGCGATG TGATTCATCT TCTCGACACC ACGATGCGCC AGGCGAGAAT TTGTGGTCTC 
    AACGCGGCGC TCACAATTCA ACTGTGCGGG CATCTCCTCC ACATGGTCAA TGCCTTTGTG 
    TTCAACAGCT TGGTCTCAGT CAACCTTCCA GCTGCCCAGT TGAATACTCG CCTCGGAAAG 
    TGTCTTCAGT ATCGGATAGA GTCGATTCAC AGATTCTGCG AACGAATGGG CGTCGAGATG 
    GCCGCCGAGT GCCATTTGGA TAGATCCCGT CAGGCCGCTA ACCTTCTCGC CACGCAGAAG 
    AATGATGTTG CCGCGTTGGG ATCGACGTGC TATAAGCTGA ACAGCCTCCA AGTGGTTCAT 
    ATCCTGTCGG GATTCGAACC AGACGACGGC GAGATTCCAT GTGACGATGA CGTCGTTGAT 
    CGGATCTGTA GACTCGCTGA AAAACAGGCG GATAAGCTGA CGTTGGAGGA TGGGCAGCGC 
    GTGACACTGG AGGAGTCCGA CGAGCTGAAC CTTCCGTTCC TGATCCCTCA GGATGGTTAC 
    TTTGTCGAGC AGTTCCGAAC GGTGCCAGAT GGTCTTTGGC AGTACTTGCA GCAACTTCAG 
    CATAGGCGCC TCTGTCATAC GGTGTCGCTT ACACCGGCAC CGCCGGCTGC GATGACCCAT 
    TCGATGCATG CGATGCATAT TGGAGCAAGC GATGCTCATT ATCATCAGCA TCAGCATCAG 
    CAGCAGCAGA ACCCGATTGT GAGAAGTGTG TCGCAGACTA CACTGACCAG TATTCCGACT 
    ACACAATTTG GCGACAGTTT TGGAAGTGCC GTGTCGGATA CGATATCGAA GGTGACACTG 
    CGGAAGAATG GAGGAGGCAT CGGATTGAGT ATTGTCGCTG CTCAGGGTGT TGGTGATCGT 
    CAAATGGGAA TTTATGTGAA GAAGGTTGTC GAAGGAACAC CGGCTGCACA CGATGGACGA 
    CTCGAGACTG GTGATCAGTT ATTGAGTGTT AACGGACATT CGTTAATTGG AATTTCACAG 
    GAAGACGCCG CCCGTCTCAT GACCCAAAGT GGAAACGAGG TGCATTTCGA CGTCCGGAAA 
    AGAGCCGCTC ACCGTAACGG GCTCAGCAAT TGGCTCGGAT CCCAAACACC GACAACTTCA 
    AATTTCATTG CACCATCTCC ATCTACAGTA CCCCCGCCGA GTTATTCACA TGTTGGACGG 
    ACTCCGTCTT ATGATGGACA ACCACCACCG AGTTATAATC ACAGAAATGG TGGAGCCAGC 
    GGAGCAGGAG GATCATCTGG AAGAAATAGT GCTTTCGTAG CACCACTTTC GAATTCATCA 
    TCAAATGCGT CGAGTAGTCA TATGCAACAA CACTATGCTC ATCATAATAT GATGCATAAT 
    GGCAATTTGA GTGCTGCTCC GGGAGCTCCC GCGGGGGATC CGAAACATTA TCGAAGCTCG 
    TCGGCTAGTG ATTTGCAGCA CGATCCAAAC GCCTCATTCT CTCAATCATC AATGACTGGA 
    AGTGCGAGAA GTTCAGTGTT TGACAAGCTT CCATCTCACT ACCGTCACTC GACACGGCCT 
    ACAGTAATCC AGCCAACACG TCCAAGTGCT CAATCTCCAT CGGCTCTTCG AAAAGCGGCC 
    GCCGCCTCGC CGGTCAGCAA TCTGTACCGT CCGACGTCGG CGTCGAATCT GTTTGCACCG 
    CCCAGAAATT CGGGCGGTCT CGTCGGATAT TCGAGTAACG CGGCACGGGA GTCGACACAG 
    GATATTCATC GAGCGCTAAA TGACCTGGAA CTCAACTCAC CGCTCGCCAG ATCCACGCCG 
    AATGAGGAGT ACCGTATCCC GCCGCCTAGA GTACCTACAG TAACCTCCAC CTCCTCCAAC 
    TATGCCCCTC TTCATTCTAT TCGACCGGCA AGCATGTATG GATATCAGAG CCCGAGCTCT 
    TCTACTACAG TAATCCCGGC TCCAAAAGGA GTGGCTTCTT CCTCTAATAA CACTTCTCCC 
    CGAGTGAATG TGACGTTGGG TGGAGCATTG AGCTCATCGA GATCTCATAA TAGTCTTTCA 
    CAGGAGCCAG CCATTGTTAT CAGGCCTGTC GACCTGAATA TGCCGAAAGC GACACCACCG 
    GCGTCGATTC GTGGAGCTGT GCCGACGGCG ATTACACAGG CTCTTCAACT TCAACCAAAC 
    GGCGGTGGAG CAGTACTTCG TCAGGTTCGG CCAGCATCCG GGCTCCGCGA GCGACTTCAG 
    GACGCTGATG TTCGGAATTT GGAGAAAACG AACACAGCAC TGATGAGCTA TGAAGCAGTG 
    AATGAGGAGC TCGATCGACT CGACGCTAAG CAGAATACGA TGACAATTGA CGAGCAACGG 
    AGATATCGAG AGCTACTGAA CGTTGTCGCC GAGCAGAGCC GAGCAAGGAA GCTGCTCTCG 
    GAGATCAGAT CACCACCTAC ATCTTATCAA GAACGTGAAA TTCCGATTCA TGTGCAAAAA 
    GAGCAGCAGC AGCACCGGCG GGAAACTGTG ATTGATGATA TTCCACACTC GAGCTCAAGT 
    GTTGAGAATG TGTCACCTGT GAGCCCGCCG GAAAGTCGAA AAGTTCAATT TATTGATGAA 
    GTTGTGCAGT ATCAGACGCC GACCAGCGGA GCCAATCACG CTTTCTTTGA TGATGTGCCG 
    GTTTTGGATT CGCCTGGGAT CGTCGGCACC AACGAAATCT ACCGTGATCC CCGCCAACGT 
    CGGCTCAACG AGCTCCAAGA CCGCAATCGC AGCTCGGAAA ATGGAGCGGC GGACGGCGCG 
    AAGCTCGGCT TCCGCGACAA GCAGCGCCTA TTCGCACGGC AAATCGGCGA GGACACGGCA 
    CCACGCCAAC GAATGAATGA GAGCAGTGCT CAGCGGCTCA TTGAGACGGA CATTGCCGCC 
    ACCCCGCAAA AGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306408.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293337.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.
    Price & Availability↑
    CloneID OCa336169
    Clone ID Related Accession (Same CDS sequence) NM_001306408.2 , NM_001306408.1
    Accession Version NM_001306408.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306408.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTCCGGCT CATCAGTCTC ATCATCACGG CAGGAGGATC TTATGATGCA TTCCAATAAT 
    AACATCCTGC CACCGTACTC TACAAAATCC GCGCAGGAAC CCCTCCAACT GCCGCTCTCA 
    ATTCAAATCG GCGACTACAA TGTATGGGAT TTCCTGGCCG AAGTGATGTC GAATCGTGCG 
    GCAACCTCAT CGGAAATGTC CACATTCCGC CTATCGCCTG CCTACTCTCT CTACCTGGCA 
    CTTCGATCAT TCCACACACA CAATAAACCG TACTCTGTGC AATTCTTCTC ACGGATCGAG 
    TCGAATTTCA AGCAAATCGC TCAGGAATGC TCGTCGAAGG ATGATCTGAT CTTTTGGCTT 
    GCCAACGCAT CGGAATTCAA TCATCTTGTC GAGCGGGACA CTGAGCTGAA AGCGTCGAGA 
    TATGGCAAAA TATCTGTGGA AATTGAGAGA ATATTCCGGC GATTGGTTGC AGTGCTTCAA 
    AGTGTCATCC GTCCGGCGGC GCGGGCTCTG TTGGACATTC ATCTGAATGA TCACGACGCG 
    ACCGGCGATG TGATTCATCT TCTCGACACC ACGATGCGCC AGGCGAGAAT TTGTGGTCTC 
    AACGCGGCGC TCACAATTCA ACTGTGCGGG CATCTCCTCC ACATGGTCAA TGCCTTTGTG 
    TTCAACAGCT TGGTCTCAGT CAACCTTCCA GCTGCCCAGT TGAATACTCG CCTCGGAAAG 
    TGTCTTCAGT ATCGGATAGA GTCGATTCAC AGATTCTGCG AACGAATGGG CGTCGAGATG 
    GCCGCCGAGT GCCATTTGGA TAGATCCCGT CAGGCCGCTA ACCTTCTCGC CACGCAGAAG 
    AATGATGTTG CCGCGTTGGG ATCGACGTGC TATAAGCTGA ACAGCCTCCA AGTGGTTCAT 
    ATCCTGTCGG GATTCGAACC AGACGACGGC GAGATTCCAT GTGACGATGA CGTCGTTGAT 
    CGGATCTGTA GACTCGCTGA AAAACAGGCG GATAAGCTGA CGTTGGAGGA TGGGCAGCGC 
    GTGACACTGG AGGAGTCCGA CGAGCTGAAC CTTCCGTTCC TGATCCCTCA GGATGGTTAC 
    TTTGTCGAGC AGTTCCGAAC GGTGCCAGAT GGTCTTTGGC AGTACTTGCA GCAACTTCAG 
    CATAGGCGCC TCTGTCATAC GGTGTCGCTT ACACCGGCAC CGCCGGCTGC GATGACCCAT 
    TCGATGCATG CGATGCATAT TGGAGCAAGC GATGCTCATT ATCATCAGCA TCAGCATCAG 
    CAGCAGCAGA ACCCGATTGT GAGAAGTGTG TCGCAGACTA CACTGACCAG TATTCCGACT 
    ACACAATTTG GCGACAGTTT TGGAAGTGCC GTGTCGGATA CGATATCGAA GGTGACACTG 
    CGGAAGAATG GAGGAGGCAT CGGATTGAGT ATTGTCGCTG CTCAGGGTGT TGGTGATCGT 
    CAAATGGGAA TTTATGTGAA GAAGGTTGTC GAAGGAACAC CGGCTGCACA CGATGGACGA 
    CTCGAGACTG GTGATCAGTT ATTGAGTGTT AACGGACATT CGTTAATTGG AATTTCACAG 
    GAAGACGCCG CCCGTCTCAT GACCCAAAGT GGAAACGAGG TGCATTTCGA CGTCCGGAAA 
    AGAGCCGCTC ACCGTAACGG GCTCAGCAAT TGGCTCGGAT CCCAAACACC GACAACTTCA 
    AATTTCATTG CACCATCTCC ATCTACAGTA CCCCCGCCGA GTTATTCACA TGTTGGACGG 
    ACTCCGTCTT ATGATGGACA ACCACCACCG AGTTATAATC ACAGAAATGG TGGAGCCAGC 
    GGAGCAGGAG GATCATCTGG AAGAAATAGT GCTTTCGTAG CACCACTTTC GAATTCATCA 
    TCAAATGCGT CGAGTAGTCA TATGCAACAA CACTATGCTC ATCATAATAT GATGCATAAT 
    GGCAATTTGA GTGCTGCTCC GGGAGCTCCC GCGGGGGATC CGAAACATTA TCGAAGCTCG 
    TCGGCTAGTG ATTTGCAGCA CGATCCAAAC GCCTCATTCT CTCAATCATC AATGACTGGA 
    AGTGCGAGAA GTTCAGTGTT TGACAAGCTT CCATCTCACT ACCGTCACTC GACACGGCCT 
    ACAGTAATCC AGCCAACACG TCCAAGTGCT CAATCTCCAT CGGCTCTTCG AAAAGCGGCC 
    GCCGCCTCGC CGGTCAGCAA TCTGTACCGT CCGACGTCGG CGTCGAATCT GTTTGCACCG 
    CCCAGAAATT CGGGCGGTCT CGTCGGATAT TCGAGTAACG CGGCACGGGA GTCGACACAG 
    GATATTCATC GAGCGGAGCC AGCCATTGTT ATCAGGCCTG TCGACCTGAA TATGCCGAAA 
    GCGACACCAC CGGCGTCGAT TCGTGGAGCT GTGCCGACGG CGATTACACA GGCTCTTCAA 
    CTTCAACCAA ACGGCGGTGG AGCAGTACTT CGTCAGGTTC GGCCAGCATC CGGGCTCCGC 
    GAGCGACTTC AGGACGCTGA TGTTCGGAAT TTGGAGAAAA CGAACACAGC ACTGATGAGC 
    TATGAAGCAG TGAATGAGGA GCTCGATCGA CTCGACGCTA AGCAGAATAC GATGACAATT 
    GACGAGCAAC GGAGATATCG AGAGCTACTG AACGTTGTCG CCGAGCAGAG CCGAGCAAGG 
    AAGCTGCTCT CGGAGATCAG ATCACCACCT ACATCTTATC AAGAACGTGA AATTCCGATT 
    CATGTGCAAA AAGAGCAGCA GCAGCACCGG CGGGAAACTG TGATTGATGA TATTCCACAC 
    TCGAGCTCAA GTGTTGAGAA TGTGTCACCT GTGAGCCCGC CGGAAAGTCG AAAAGTTCAA 
    TTTATTGATG AAGTTGTGCA GTATCAGACG CCGACCAGCG GAGCCAATCA CGCTTTCTTT 
    GATGATGTGC CGGTTTTGGA TTCGCCTGGG ATCGTCGGCA CCAACGAAAT CTACCGTGAT 
    CCCCGCCAAC GTCGGCTCAA CGAGCTCCAA GACCGCAATC GCAGCTCGGA AAATGGAGCG 
    GCGGACGGCG CGAAGCTCGG CTTCCGCGAC AAGCAGCGCC TATTCGCACG GCAAATCGGC 
    GAGGACACGG CACCACGCCA ACGAATGAAT GAGAGCAGTG CTCAGCGGCT CATTGAGACG 
    GACATTGCCG CCACCCCGCA AAAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293337.1
    CDS2..3151
    Translation

    Target ORF information:

    RefSeq Version NM_001306408.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306408.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTCCGGCT CATCAGTCTC ATCATCACGG CAGGAGGATC TTATGATGCA TTCCAATAAT 
    AACATCCTGC CACCGTACTC TACAAAATCC GCGCAGGAAC CCCTCCAACT GCCGCTCTCA 
    ATTCAAATCG GCGACTACAA TGTATGGGAT TTCCTGGCCG AAGTGATGTC GAATCGTGCG 
    GCAACCTCAT CGGAAATGTC CACATTCCGC CTATCGCCTG CCTACTCTCT CTACCTGGCA 
    CTTCGATCAT TCCACACACA CAATAAACCG TACTCTGTGC AATTCTTCTC ACGGATCGAG 
    TCGAATTTCA AGCAAATCGC TCAGGAATGC TCGTCGAAGG ATGATCTGAT CTTTTGGCTT 
    GCCAACGCAT CGGAATTCAA TCATCTTGTC GAGCGGGACA CTGAGCTGAA AGCGTCGAGA 
    TATGGCAAAA TATCTGTGGA AATTGAGAGA ATATTCCGGC GATTGGTTGC AGTGCTTCAA 
    AGTGTCATCC GTCCGGCGGC GCGGGCTCTG TTGGACATTC ATCTGAATGA TCACGACGCG 
    ACCGGCGATG TGATTCATCT TCTCGACACC ACGATGCGCC AGGCGAGAAT TTGTGGTCTC 
    AACGCGGCGC TCACAATTCA ACTGTGCGGG CATCTCCTCC ACATGGTCAA TGCCTTTGTG 
    TTCAACAGCT TGGTCTCAGT CAACCTTCCA GCTGCCCAGT TGAATACTCG CCTCGGAAAG 
    TGTCTTCAGT ATCGGATAGA GTCGATTCAC AGATTCTGCG AACGAATGGG CGTCGAGATG 
    GCCGCCGAGT GCCATTTGGA TAGATCCCGT CAGGCCGCTA ACCTTCTCGC CACGCAGAAG 
    AATGATGTTG CCGCGTTGGG ATCGACGTGC TATAAGCTGA ACAGCCTCCA AGTGGTTCAT 
    ATCCTGTCGG GATTCGAACC AGACGACGGC GAGATTCCAT GTGACGATGA CGTCGTTGAT 
    CGGATCTGTA GACTCGCTGA AAAACAGGCG GATAAGCTGA CGTTGGAGGA TGGGCAGCGC 
    GTGACACTGG AGGAGTCCGA CGAGCTGAAC CTTCCGTTCC TGATCCCTCA GGATGGTTAC 
    TTTGTCGAGC AGTTCCGAAC GGTGCCAGAT GGTCTTTGGC AGTACTTGCA GCAACTTCAG 
    CATAGGCGCC TCTGTCATAC GGTGTCGCTT ACACCGGCAC CGCCGGCTGC GATGACCCAT 
    TCGATGCATG CGATGCATAT TGGAGCAAGC GATGCTCATT ATCATCAGCA TCAGCATCAG 
    CAGCAGCAGA ACCCGATTGT GAGAAGTGTG TCGCAGACTA CACTGACCAG TATTCCGACT 
    ACACAATTTG GCGACAGTTT TGGAAGTGCC GTGTCGGATA CGATATCGAA GGTGACACTG 
    CGGAAGAATG GAGGAGGCAT CGGATTGAGT ATTGTCGCTG CTCAGGGTGT TGGTGATCGT 
    CAAATGGGAA TTTATGTGAA GAAGGTTGTC GAAGGAACAC CGGCTGCACA CGATGGACGA 
    CTCGAGACTG GTGATCAGTT ATTGAGTGTT AACGGACATT CGTTAATTGG AATTTCACAG 
    GAAGACGCCG CCCGTCTCAT GACCCAAAGT GGAAACGAGG TGCATTTCGA CGTCCGGAAA 
    AGAGCCGCTC ACCGTAACGG GCTCAGCAAT TGGCTCGGAT CCCAAACACC GACAACTTCA 
    AATTTCATTG CACCATCTCC ATCTACAGTA CCCCCGCCGA GTTATTCACA TGTTGGACGG 
    ACTCCGTCTT ATGATGGACA ACCACCACCG AGTTATAATC ACAGAAATGG TGGAGCCAGC 
    GGAGCAGGAG GATCATCTGG AAGAAATAGT GCTTTCGTAG CACCACTTTC GAATTCATCA 
    TCAAATGCGT CGAGTAGTCA TATGCAACAA CACTATGCTC ATCATAATAT GATGCATAAT 
    GGCAATTTGA GTGCTGCTCC GGGAGCTCCC GCGGGGGATC CGAAACATTA TCGAAGCTCG 
    TCGGCTAGTG ATTTGCAGCA CGATCCAAAC GCCTCATTCT CTCAATCATC AATGACTGGA 
    AGTGCGAGAA GTTCAGTGTT TGACAAGCTT CCATCTCACT ACCGTCACTC GACACGGCCT 
    ACAGTAATCC AGCCAACACG TCCAAGTGCT CAATCTCCAT CGGCTCTTCG AAAAGCGGCC 
    GCCGCCTCGC CGGTCAGCAA TCTGTACCGT CCGACGTCGG CGTCGAATCT GTTTGCACCG 
    CCCAGAAATT CGGGCGGTCT CGTCGGATAT TCGAGTAACG CGGCACGGGA GTCGACACAG 
    GATATTCATC GAGCGGAGCC AGCCATTGTT ATCAGGCCTG TCGACCTGAA TATGCCGAAA 
    GCGACACCAC CGGCGTCGAT TCGTGGAGCT GTGCCGACGG CGATTACACA GGCTCTTCAA 
    CTTCAACCAA ACGGCGGTGG AGCAGTACTT CGTCAGGTTC GGCCAGCATC CGGGCTCCGC 
    GAGCGACTTC AGGACGCTGA TGTTCGGAAT TTGGAGAAAA CGAACACAGC ACTGATGAGC 
    TATGAAGCAG TGAATGAGGA GCTCGATCGA CTCGACGCTA AGCAGAATAC GATGACAATT 
    GACGAGCAAC GGAGATATCG AGAGCTACTG AACGTTGTCG CCGAGCAGAG CCGAGCAAGG 
    AAGCTGCTCT CGGAGATCAG ATCACCACCT ACATCTTATC AAGAACGTGA AATTCCGATT 
    CATGTGCAAA AAGAGCAGCA GCAGCACCGG CGGGAAACTG TGATTGATGA TATTCCACAC 
    TCGAGCTCAA GTGTTGAGAA TGTGTCACCT GTGAGCCCGC CGGAAAGTCG AAAAGTTCAA 
    TTTATTGATG AAGTTGTGCA GTATCAGACG CCGACCAGCG GAGCCAATCA CGCTTTCTTT 
    GATGATGTGC CGGTTTTGGA TTCGCCTGGG ATCGTCGGCA CCAACGAAAT CTACCGTGAT 
    CCCCGCCAAC GTCGGCTCAA CGAGCTCCAA GACCGCAATC GCAGCTCGGA AAATGGAGCG 
    GCGGACGGCG CGAAGCTCGG CTTCCGCGAC AAGCAGCGCC TATTCGCACG GCAAATCGGC 
    GAGGACACGG CACCACGCCA ACGAATGAAT GAGAGCAGTG CTCAGCGGCT CATTGAGACG 
    GACATTGCCG CCACCCCGCA AAAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306408.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293337.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336169
    Clone ID Related Accession (Same CDS sequence) NM_001306408.2 , NM_001306408.1
    Accession Version NM_001306408.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTCCGGCT CATCAGTCTC ATCATCACGG CAGGAGGATC TTATGATGCA TTCCAATAAT 
    AACATCCTGC CACCGTACTC TACAAAATCC GCGCAGGAAC CCCTCCAACT GCCGCTCTCA 
    ATTCAAATCG GCGACTACAA TGTATGGGAT TTCCTGGCCG AAGTGATGTC GAATCGTGCG 
    GCAACCTCAT CGGAAATGTC CACATTCCGC CTATCGCCTG CCTACTCTCT CTACCTGGCA 
    CTTCGATCAT TCCACACACA CAATAAACCG TACTCTGTGC AATTCTTCTC ACGGATCGAG 
    TCGAATTTCA AGCAAATCGC TCAGGAATGC TCGTCGAAGG ATGATCTGAT CTTTTGGCTT 
    GCCAACGCAT CGGAATTCAA TCATCTTGTC GAGCGGGACA CTGAGCTGAA AGCGTCGAGA 
    TATGGCAAAA TATCTGTGGA AATTGAGAGA ATATTCCGGC GATTGGTTGC AGTGCTTCAA 
    AGTGTCATCC GTCCGGCGGC GCGGGCTCTG TTGGACATTC ATCTGAATGA TCACGACGCG 
    ACCGGCGATG TGATTCATCT TCTCGACACC ACGATGCGCC AGGCGAGAAT TTGTGGTCTC 
    AACGCGGCGC TCACAATTCA ACTGTGCGGG CATCTCCTCC ACATGGTCAA TGCCTTTGTG 
    TTCAACAGCT TGGTCTCAGT CAACCTTCCA GCTGCCCAGT TGAATACTCG CCTCGGAAAG 
    TGTCTTCAGT ATCGGATAGA GTCGATTCAC AGATTCTGCG AACGAATGGG CGTCGAGATG 
    GCCGCCGAGT GCCATTTGGA TAGATCCCGT CAGGCCGCTA ACCTTCTCGC CACGCAGAAG 
    AATGATGTTG CCGCGTTGGG ATCGACGTGC TATAAGCTGA ACAGCCTCCA AGTGGTTCAT 
    ATCCTGTCGG GATTCGAACC AGACGACGGC GAGATTCCAT GTGACGATGA CGTCGTTGAT 
    CGGATCTGTA GACTCGCTGA AAAACAGGCG GATAAGCTGA CGTTGGAGGA TGGGCAGCGC 
    GTGACACTGG AGGAGTCCGA CGAGCTGAAC CTTCCGTTCC TGATCCCTCA GGATGGTTAC 
    TTTGTCGAGC AGTTCCGAAC GGTGCCAGAT GGTCTTTGGC AGTACTTGCA GCAACTTCAG 
    CATAGGCGCC TCTGTCATAC GGTGTCGCTT ACACCGGCAC CGCCGGCTGC GATGACCCAT 
    TCGATGCATG CGATGCATAT TGGAGCAAGC GATGCTCATT ATCATCAGCA TCAGCATCAG 
    CAGCAGCAGA ACCCGATTGT GAGAAGTGTG TCGCAGACTA CACTGACCAG TATTCCGACT 
    ACACAATTTG GCGACAGTTT TGGAAGTGCC GTGTCGGATA CGATATCGAA GGTGACACTG 
    CGGAAGAATG GAGGAGGCAT CGGATTGAGT ATTGTCGCTG CTCAGGGTGT TGGTGATCGT 
    CAAATGGGAA TTTATGTGAA GAAGGTTGTC GAAGGAACAC CGGCTGCACA CGATGGACGA 
    CTCGAGACTG GTGATCAGTT ATTGAGTGTT AACGGACATT CGTTAATTGG AATTTCACAG 
    GAAGACGCCG CCCGTCTCAT GACCCAAAGT GGAAACGAGG TGCATTTCGA CGTCCGGAAA 
    AGAGCCGCTC ACCGTAACGG GCTCAGCAAT TGGCTCGGAT CCCAAACACC GACAACTTCA 
    AATTTCATTG CACCATCTCC ATCTACAGTA CCCCCGCCGA GTTATTCACA TGTTGGACGG 
    ACTCCGTCTT ATGATGGACA ACCACCACCG AGTTATAATC ACAGAAATGG TGGAGCCAGC 
    GGAGCAGGAG GATCATCTGG AAGAAATAGT GCTTTCGTAG CACCACTTTC GAATTCATCA 
    TCAAATGCGT CGAGTAGTCA TATGCAACAA CACTATGCTC ATCATAATAT GATGCATAAT 
    GGCAATTTGA GTGCTGCTCC GGGAGCTCCC GCGGGGGATC CGAAACATTA TCGAAGCTCG 
    TCGGCTAGTG ATTTGCAGCA CGATCCAAAC GCCTCATTCT CTCAATCATC AATGACTGGA 
    AGTGCGAGAA GTTCAGTGTT TGACAAGCTT CCATCTCACT ACCGTCACTC GACACGGCCT 
    ACAGTAATCC AGCCAACACG TCCAAGTGCT CAATCTCCAT CGGCTCTTCG AAAAGCGGCC 
    GCCGCCTCGC CGGTCAGCAA TCTGTACCGT CCGACGTCGG CGTCGAATCT GTTTGCACCG 
    CCCAGAAATT CGGGCGGTCT CGTCGGATAT TCGAGTAACG CGGCACGGGA GTCGACACAG 
    GATATTCATC GAGCGGAGCC AGCCATTGTT ATCAGGCCTG TCGACCTGAA TATGCCGAAA 
    GCGACACCAC CGGCGTCGAT TCGTGGAGCT GTGCCGACGG CGATTACACA GGCTCTTCAA 
    CTTCAACCAA ACGGCGGTGG AGCAGTACTT CGTCAGGTTC GGCCAGCATC CGGGCTCCGC 
    GAGCGACTTC AGGACGCTGA TGTTCGGAAT TTGGAGAAAA CGAACACAGC ACTGATGAGC 
    TATGAAGCAG TGAATGAGGA GCTCGATCGA CTCGACGCTA AGCAGAATAC GATGACAATT 
    GACGAGCAAC GGAGATATCG AGAGCTACTG AACGTTGTCG CCGAGCAGAG CCGAGCAAGG 
    AAGCTGCTCT CGGAGATCAG ATCACCACCT ACATCTTATC AAGAACGTGA AATTCCGATT 
    CATGTGCAAA AAGAGCAGCA GCAGCACCGG CGGGAAACTG TGATTGATGA TATTCCACAC 
    TCGAGCTCAA GTGTTGAGAA TGTGTCACCT GTGAGCCCGC CGGAAAGTCG AAAAGTTCAA 
    TTTATTGATG AAGTTGTGCA GTATCAGACG CCGACCAGCG GAGCCAATCA CGCTTTCTTT 
    GATGATGTGC CGGTTTTGGA TTCGCCTGGG ATCGTCGGCA CCAACGAAAT CTACCGTGAT 
    CCCCGCCAAC GTCGGCTCAA CGAGCTCCAA GACCGCAATC GCAGCTCGGA AAATGGAGCG 
    GCGGACGGCG CGAAGCTCGG CTTCCGCGAC AAGCAGCGCC TATTCGCACG GCAAATCGGC 
    GAGGACACGG CACCACGCCA ACGAATGAAT GAGAGCAGTG CTCAGCGGCT CATTGAGACG 
    GACATTGCCG CCACCCCGCA AAAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293337.1
    CDS1..3150
    Translation

    Target ORF information:

    RefSeq Version NM_001306408.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTCCGGCT CATCAGTCTC ATCATCACGG CAGGAGGATC TTATGATGCA TTCCAATAAT 
    AACATCCTGC CACCGTACTC TACAAAATCC GCGCAGGAAC CCCTCCAACT GCCGCTCTCA 
    ATTCAAATCG GCGACTACAA TGTATGGGAT TTCCTGGCCG AAGTGATGTC GAATCGTGCG 
    GCAACCTCAT CGGAAATGTC CACATTCCGC CTATCGCCTG CCTACTCTCT CTACCTGGCA 
    CTTCGATCAT TCCACACACA CAATAAACCG TACTCTGTGC AATTCTTCTC ACGGATCGAG 
    TCGAATTTCA AGCAAATCGC TCAGGAATGC TCGTCGAAGG ATGATCTGAT CTTTTGGCTT 
    GCCAACGCAT CGGAATTCAA TCATCTTGTC GAGCGGGACA CTGAGCTGAA AGCGTCGAGA 
    TATGGCAAAA TATCTGTGGA AATTGAGAGA ATATTCCGGC GATTGGTTGC AGTGCTTCAA 
    AGTGTCATCC GTCCGGCGGC GCGGGCTCTG TTGGACATTC ATCTGAATGA TCACGACGCG 
    ACCGGCGATG TGATTCATCT TCTCGACACC ACGATGCGCC AGGCGAGAAT TTGTGGTCTC 
    AACGCGGCGC TCACAATTCA ACTGTGCGGG CATCTCCTCC ACATGGTCAA TGCCTTTGTG 
    TTCAACAGCT TGGTCTCAGT CAACCTTCCA GCTGCCCAGT TGAATACTCG CCTCGGAAAG 
    TGTCTTCAGT ATCGGATAGA GTCGATTCAC AGATTCTGCG AACGAATGGG CGTCGAGATG 
    GCCGCCGAGT GCCATTTGGA TAGATCCCGT CAGGCCGCTA ACCTTCTCGC CACGCAGAAG 
    AATGATGTTG CCGCGTTGGG ATCGACGTGC TATAAGCTGA ACAGCCTCCA AGTGGTTCAT 
    ATCCTGTCGG GATTCGAACC AGACGACGGC GAGATTCCAT GTGACGATGA CGTCGTTGAT 
    CGGATCTGTA GACTCGCTGA AAAACAGGCG GATAAGCTGA CGTTGGAGGA TGGGCAGCGC 
    GTGACACTGG AGGAGTCCGA CGAGCTGAAC CTTCCGTTCC TGATCCCTCA GGATGGTTAC 
    TTTGTCGAGC AGTTCCGAAC GGTGCCAGAT GGTCTTTGGC AGTACTTGCA GCAACTTCAG 
    CATAGGCGCC TCTGTCATAC GGTGTCGCTT ACACCGGCAC CGCCGGCTGC GATGACCCAT 
    TCGATGCATG CGATGCATAT TGGAGCAAGC GATGCTCATT ATCATCAGCA TCAGCATCAG 
    CAGCAGCAGA ACCCGATTGT GAGAAGTGTG TCGCAGACTA CACTGACCAG TATTCCGACT 
    ACACAATTTG GCGACAGTTT TGGAAGTGCC GTGTCGGATA CGATATCGAA GGTGACACTG 
    CGGAAGAATG GAGGAGGCAT CGGATTGAGT ATTGTCGCTG CTCAGGGTGT TGGTGATCGT 
    CAAATGGGAA TTTATGTGAA GAAGGTTGTC GAAGGAACAC CGGCTGCACA CGATGGACGA 
    CTCGAGACTG GTGATCAGTT ATTGAGTGTT AACGGACATT CGTTAATTGG AATTTCACAG 
    GAAGACGCCG CCCGTCTCAT GACCCAAAGT GGAAACGAGG TGCATTTCGA CGTCCGGAAA 
    AGAGCCGCTC ACCGTAACGG GCTCAGCAAT TGGCTCGGAT CCCAAACACC GACAACTTCA 
    AATTTCATTG CACCATCTCC ATCTACAGTA CCCCCGCCGA GTTATTCACA TGTTGGACGG 
    ACTCCGTCTT ATGATGGACA ACCACCACCG AGTTATAATC ACAGAAATGG TGGAGCCAGC 
    GGAGCAGGAG GATCATCTGG AAGAAATAGT GCTTTCGTAG CACCACTTTC GAATTCATCA 
    TCAAATGCGT CGAGTAGTCA TATGCAACAA CACTATGCTC ATCATAATAT GATGCATAAT 
    GGCAATTTGA GTGCTGCTCC GGGAGCTCCC GCGGGGGATC CGAAACATTA TCGAAGCTCG 
    TCGGCTAGTG ATTTGCAGCA CGATCCAAAC GCCTCATTCT CTCAATCATC AATGACTGGA 
    AGTGCGAGAA GTTCAGTGTT TGACAAGCTT CCATCTCACT ACCGTCACTC GACACGGCCT 
    ACAGTAATCC AGCCAACACG TCCAAGTGCT CAATCTCCAT CGGCTCTTCG AAAAGCGGCC 
    GCCGCCTCGC CGGTCAGCAA TCTGTACCGT CCGACGTCGG CGTCGAATCT GTTTGCACCG 
    CCCAGAAATT CGGGCGGTCT CGTCGGATAT TCGAGTAACG CGGCACGGGA GTCGACACAG 
    GATATTCATC GAGCGGAGCC AGCCATTGTT ATCAGGCCTG TCGACCTGAA TATGCCGAAA 
    GCGACACCAC CGGCGTCGAT TCGTGGAGCT GTGCCGACGG CGATTACACA GGCTCTTCAA 
    CTTCAACCAA ACGGCGGTGG AGCAGTACTT CGTCAGGTTC GGCCAGCATC CGGGCTCCGC 
    GAGCGACTTC AGGACGCTGA TGTTCGGAAT TTGGAGAAAA CGAACACAGC ACTGATGAGC 
    TATGAAGCAG TGAATGAGGA GCTCGATCGA CTCGACGCTA AGCAGAATAC GATGACAATT 
    GACGAGCAAC GGAGATATCG AGAGCTACTG AACGTTGTCG CCGAGCAGAG CCGAGCAAGG 
    AAGCTGCTCT CGGAGATCAG ATCACCACCT ACATCTTATC AAGAACGTGA AATTCCGATT 
    CATGTGCAAA AAGAGCAGCA GCAGCACCGG CGGGAAACTG TGATTGATGA TATTCCACAC 
    TCGAGCTCAA GTGTTGAGAA TGTGTCACCT GTGAGCCCGC CGGAAAGTCG AAAAGTTCAA 
    TTTATTGATG AAGTTGTGCA GTATCAGACG CCGACCAGCG GAGCCAATCA CGCTTTCTTT 
    GATGATGTGC CGGTTTTGGA TTCGCCTGGG ATCGTCGGCA CCAACGAAAT CTACCGTGAT 
    CCCCGCCAAC GTCGGCTCAA CGAGCTCCAA GACCGCAATC GCAGCTCGGA AAATGGAGCG 
    GCGGACGGCG CGAAGCTCGG CTTCCGCGAC AAGCAGCGCC TATTCGCACG GCAAATCGGC 
    GAGGACACGG CACCACGCCA ACGAATGAAT GAGAGCAGTG CTCAGCGGCT CATTGAGACG 
    GACATTGCCG CCACCCCGCA AAAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_058537.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_490938.3 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336875
    Clone ID Related Accession (Same CDS sequence) NM_058537.4 , NM_058537.5
    Accession Version NM_058537.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_058537.3. COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTAC AAGTAAACGG AGGACGAGAA GAAGCGCAAT CTCAAGATGA TATCTATAAA 
    CAAGTACCAT TTAATACATT CACCCGAACT ATATCTAATC CAGAAGCTGT TATGAGAAAA 
    CGGAGGGAAA AGAAATTGGA GACGAAATTG AAGGAAATGA TGTTTGGCGG AAGTCTCAAA 
    GTATACGGTG GAGAAATTGT GCCAACTCGT CCATATGTCT CAATATTGGC TGAAATCAAC 
    GAAAATGCTG ATAGAATTTT GGGTGCTGCA CTGGAAAAGT ATGGATTGGA ACATTCGAAA 
    GATGATTTTA TTCTTGTTGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_490938.3
    CDS1..4977
    Translation

    Target ORF information:

    RefSeq Version NM_058537.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_058537.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTAC AAGTAAACGG AGGACGAGAA GAAGCGCAAT CTCAAGATGA TATCTATAAA 
    CAAGTACCAT TTAATACATT CACCCGAACT ATATCTAATC CAGAAGCTGT TATGAGAAAA 
    CGGAGGGAAA AGAAATTGGA GACGAAATTG AAGGAAATGA TGTTTGGCGG AAGTCTCAAA 
    GTATACGGTG GAGAAATTGT GCCAACTCGT CCATATGTCT CAATATTGGC TGAAATCAAC 
    GAAAATGCTG ATAGAATTTT GGGTGCTGCA CTGGAAAAGT ATGGATTGGA ACATTCGAAA 
    GATGATTTTA TTCTTGTTGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306529.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293458.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.
    Price & Availability↑
    CloneID OCa337252
    Clone ID Related Accession (Same CDS sequence) NM_001306529.2 , NM_001306529.1
    Accession Version NM_001306529.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306529.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGTCGAG TCAACTCGGA ATCTCGTCTC CACAAAACAG AAACCACATT TGGAGTCGGT 
    TCTTCTTCTC CAACCATCCC ATCATCTTAT GCACTTCCCA GACACCAACA AACTTCTCGT 
    TATATCTATC ATCGCCGAGC AGCATCCACA GATGCTGAGC CCCTCCTCGC ATCGTTTGAT 
    GCACCTGTGC CCCGACCCCG TCGGGTCACT TTTTCGCAGA CATCTGCGGC AAAGCCACCG 
    CCGCCAGTTC CGATACCCGT CACGAGGTTT CAGACGGCTG CACCTGCTGC GAATTTCAGT 
    GCTAGACAGC TTAAACATCT CATGTCCGGC TCATCAGTCT CATCATCACG GCAGGAGGAT 
    CTTATGATGC ATTCCAATAA TAACATCCTG CCACCGTACT CTACAAAATC CGCGCAGGAA 
    CCCCTCCAAC TGCCGCTCTC AATTCAAATC GGCGACTACA ATGTATGGGA TTTCCTGGCC 
    GAAGTGATGT CGAATCGTGC GGCAACCTCA TCGGAAATGT CCACATTCCG CCTATCGCCT 
    GCCTACTCTC TCTACCTGGC ACTTCGATCA TTCCACACAC ACAATAAACC GTACTCTGTG 
    CAATTCTTCT CACGGATCGA GTCGAATTTC AAGCAAATCG CTCAGGAATG CTCGTCGAAG 
    GATGATCTGA TCTTTTGGCT TGCCAACGCA TCGGAATTCA ATCATCTTGT CGAGCGGGAC 
    ACTGAGCTGA AAGCGTCGAG ATATGGCAAA ATATCTGTGG AAATTGAGAG AATATTCCGG 
    CGATTGGTTG CAGTGCTTCA AAGTGTCATC CGTCCGGCGG CGCGGGCTCT GTTGGACATT 
    CATCTGAATG ATCACGACGC GACCGGCGAT GTGATTCATC TTCTCGACAC CACGATGCGC 
    CAGGCGAGAA TTTGTGGTCT CAACGCGGCG CTCACAATTC AACTGTGCGG GCATCTCCTC 
    CACATGGTCA ATGCCTTTGT GTTCAACAGC TTGGTCTCAG TCAACCTTCC AGCTGCCCAG 
    TTGAATACTC GCCTCGGAAA GTGTCTTCAG TATCGGATAG AGTCGATTCA CAGATTCTGC 
    GAACGAATGG GCGTCGAGAT GGCCGCCGAG TGCCATTTGG ATAGATCCCG TCAGGCCGCT 
    AACCTTCTCG CCACGCAGAA GAATGATGTT GCCGCGTTGG GATCGACGTG CTATAAGCTG 
    AACAGCCTCC AAGTGGTTCA TATCCTGTCG GGATTCGAAC CAGACGACGG CGAGATTCCA 
    TGTGACGATG ACGTCGTTGA TCGGATCTGT AGACTCGCTG AAAAACAGGC GGATAAGCTG 
    ACGTTGGAGG ATGGGCAGCG CGTGACACTG GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC 
    CTGATCCCTC AGGATGGTTA CTTTGTCGAG CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG 
    CAGTACTTGC AGCAACTTCA GCATAGGCGC CTCTGTCATA CGGTGTCGCT TACACCGGCA 
    CCGCCGGCTG CGATGACCCA TTCGATGCAT GCGATGCATA TTGGAGCAAG CGATGCTCAT 
    TATCATCAGC ATCAGCATCA GCAGCAGCAG AACCCGATTG TGAGAAGTGT GTCGCAGACT 
    ACACTGACCA GTATTCCGAC TACACAATTT GGCGACAGTT TTGGAAGTGC CGTGTCGGAT 
    ACGATATCGA AGGTGACACT GCGGAAGAAT GGAGGAGGCA TCGGATTGAG TATTGTCGCT 
    GCTCAGGGTG TTGGTGATCG TCAAATGGGA ATTTATGTGA AGAAGGTTGT CGAAGGAACA 
    CCGGCTGCAC ACGATGGACG ACTCGAGACT GGTGATCAGT TATTGAGTGT TAACGGACAT 
    TCGTTAATTG GAATTTCACA GGAAGACGCC GCCCGTCTCA TGACCCAAAG TGGAAACGAG 
    GTGCATTTCG ACGTCCGGAA AAGAGCCGCT CACCGTAACG GGCTCAGCAA TTGGCTCGGA 
    TCCCAAACAC CGACAACTTC AAATTTCATT GCACCATCTC CATCTACAGT ACCCCCGCCG 
    AGTTATTCAC ATGTTGGACG GACTCCGTCT TATGATGGAC AACCACCACC GAGTTATAAT 
    CACAGAAATG GTGGAGCCAG CGGAGCAGGA GGATCATCTG GAAGAAATAG TGCTTTCGTA 
    GCACCACTTT CGAATTCATC ATCAAATGCG TCGAGTAGTC ATATGCAACA ACACTATGCT 
    CATCATAATA TGATGCATAA TGGCAATTTG AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT 
    CCGAAACATT ATCGAAGCTC GTCGGCTAGT GATTTGCAGC ACGATCCAAA CGCCTCATTC 
    TCTCAATCAT CAATGACTGG AAGTGCGAGA AGTTCAGTGT TTGACAAGCT TCCATCTCAC 
    TACCGTCACT CGACACGGCC TACAGTAATC CAGCCAACAC GTCCAAGTGC TCAATCTCCA 
    TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG CCGGTCAGCA ATCTGTACCG TCCGACGTCG 
    GCGTCGAATC TGTTTGCACC GCCCAGAAAT TCGGGCGGTC TCGTCGGATA TTCGAGTAAC 
    GCGGCACGGG AGTCGACACA GGATATTCAT CGAGCGCTAA ATGACCTGGA ACTCAACTCA 
    CCGCTCGCCA GATCCACGCC GAATGAGGAG TACCGTATCC CGCCGCCTAG AGTACCTACA 
    GTAACCTCCA CCTCCTCCAA CTATGCCCCT CTTCATTCTA TTCGACCGGC AAGCATGTAT 
    GGATATCAGA GCCCGAGCTC TTCTACTACA GTAATCCCGG CTCCAAAAGG AGTGGCTTCT 
    TCCTCTAATA ACACTTCTCC CCGAGTGAAT GTGACGTTGG GTGGAGCATT GAGCTCATCG 
    AGATCTCATA ATAGTCTTTC ACAGGAGCCA GCCATTGTTA TCAGGCCTGT CGACCTGAAT 
    ATGCCGAAAG CGACACCACC GGCGTCGATT CGTGGAGCTG TGCCGACGGC GATTACACAG 
    GCTCTTCAAC TTCAACCAAA CGGCGGTGGA GCAGTACTTC GTCAGGTTCG GCCAGCATCC 
    GGGCTCCGCG AGCGACTTCA GGACGCTGAT GTTCGGAATT TGGAGAAAAC GAACACAGCA 
    CTGATGAGCT ATGAAGCAGT GAATGAGGAG CTCGATCGAC TCGACGCTAA GCAGAATACG 
    ATGACAATTG ACGAGCAACG GAGATATCGA GAGCTACTGA ACGTTGTCGC CGAGCAGAGC 
    CGAGCAAGGA AGCTGCTCTC GGAGATCAGA TCACCACCTA CATCTTATCA AGAACGTGAA 
    ATTCCGATTC ATGTGCAAAA AGAGCAGCAG CAGCACCGGC GGGAAACTGT GATTGATGAT 
    ATTCCACACT CGAGCTCAAG TGTTGAGAAT GTGTCACCTG TGAGCCCGCC GGAAAGTCGA 
    AAAGTTCAAT TTATTGATGA AGTTGTGCAG TATCAGACGC CGACCAGCGG AGCCAATCAC 
    GCTTTCTTTG ATGATGTGCC GGTTTTGGAT TCGCCTGGGA TCGTCGGCAC CAACGAAATC 
    TACCGTGATC CCCGCCAACG TCGGCTCAAC GAGCTCCAAG ACCGCAATCG CAGCTCGGAA 
    AATGGAGCGG CGGACGGCGC GAAGCTCGGC TTCCGCGACA AGCAGCGCCT ATTCGCACGG 
    CAAATCGGCG AGGACACGGC ACCACGCCAA CGAATGAATG AGAGCAGTGC TCAGCGGCTC 
    ATTGAGACGG ACATTGCCGC CACCCCGCAA AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293458.1
    CDS7..3765
    Translation

    Target ORF information:

    RefSeq Version NM_001306529.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306529.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGTCGAG TCAACTCGGA ATCTCGTCTC CACAAAACAG AAACCACATT TGGAGTCGGT 
    TCTTCTTCTC CAACCATCCC ATCATCTTAT GCACTTCCCA GACACCAACA AACTTCTCGT 
    TATATCTATC ATCGCCGAGC AGCATCCACA GATGCTGAGC CCCTCCTCGC ATCGTTTGAT 
    GCACCTGTGC CCCGACCCCG TCGGGTCACT TTTTCGCAGA CATCTGCGGC AAAGCCACCG 
    CCGCCAGTTC CGATACCCGT CACGAGGTTT CAGACGGCTG CACCTGCTGC GAATTTCAGT 
    GCTAGACAGC TTAAACATCT CATGTCCGGC TCATCAGTCT CATCATCACG GCAGGAGGAT 
    CTTATGATGC ATTCCAATAA TAACATCCTG CCACCGTACT CTACAAAATC CGCGCAGGAA 
    CCCCTCCAAC TGCCGCTCTC AATTCAAATC GGCGACTACA ATGTATGGGA TTTCCTGGCC 
    GAAGTGATGT CGAATCGTGC GGCAACCTCA TCGGAAATGT CCACATTCCG CCTATCGCCT 
    GCCTACTCTC TCTACCTGGC ACTTCGATCA TTCCACACAC ACAATAAACC GTACTCTGTG 
    CAATTCTTCT CACGGATCGA GTCGAATTTC AAGCAAATCG CTCAGGAATG CTCGTCGAAG 
    GATGATCTGA TCTTTTGGCT TGCCAACGCA TCGGAATTCA ATCATCTTGT CGAGCGGGAC 
    ACTGAGCTGA AAGCGTCGAG ATATGGCAAA ATATCTGTGG AAATTGAGAG AATATTCCGG 
    CGATTGGTTG CAGTGCTTCA AAGTGTCATC CGTCCGGCGG CGCGGGCTCT GTTGGACATT 
    CATCTGAATG ATCACGACGC GACCGGCGAT GTGATTCATC TTCTCGACAC CACGATGCGC 
    CAGGCGAGAA TTTGTGGTCT CAACGCGGCG CTCACAATTC AACTGTGCGG GCATCTCCTC 
    CACATGGTCA ATGCCTTTGT GTTCAACAGC TTGGTCTCAG TCAACCTTCC AGCTGCCCAG 
    TTGAATACTC GCCTCGGAAA GTGTCTTCAG TATCGGATAG AGTCGATTCA CAGATTCTGC 
    GAACGAATGG GCGTCGAGAT GGCCGCCGAG TGCCATTTGG ATAGATCCCG TCAGGCCGCT 
    AACCTTCTCG CCACGCAGAA GAATGATGTT GCCGCGTTGG GATCGACGTG CTATAAGCTG 
    AACAGCCTCC AAGTGGTTCA TATCCTGTCG GGATTCGAAC CAGACGACGG CGAGATTCCA 
    TGTGACGATG ACGTCGTTGA TCGGATCTGT AGACTCGCTG AAAAACAGGC GGATAAGCTG 
    ACGTTGGAGG ATGGGCAGCG CGTGACACTG GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC 
    CTGATCCCTC AGGATGGTTA CTTTGTCGAG CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG 
    CAGTACTTGC AGCAACTTCA GCATAGGCGC CTCTGTCATA CGGTGTCGCT TACACCGGCA 
    CCGCCGGCTG CGATGACCCA TTCGATGCAT GCGATGCATA TTGGAGCAAG CGATGCTCAT 
    TATCATCAGC ATCAGCATCA GCAGCAGCAG AACCCGATTG TGAGAAGTGT GTCGCAGACT 
    ACACTGACCA GTATTCCGAC TACACAATTT GGCGACAGTT TTGGAAGTGC CGTGTCGGAT 
    ACGATATCGA AGGTGACACT GCGGAAGAAT GGAGGAGGCA TCGGATTGAG TATTGTCGCT 
    GCTCAGGGTG TTGGTGATCG TCAAATGGGA ATTTATGTGA AGAAGGTTGT CGAAGGAACA 
    CCGGCTGCAC ACGATGGACG ACTCGAGACT GGTGATCAGT TATTGAGTGT TAACGGACAT 
    TCGTTAATTG GAATTTCACA GGAAGACGCC GCCCGTCTCA TGACCCAAAG TGGAAACGAG 
    GTGCATTTCG ACGTCCGGAA AAGAGCCGCT CACCGTAACG GGCTCAGCAA TTGGCTCGGA 
    TCCCAAACAC CGACAACTTC AAATTTCATT GCACCATCTC CATCTACAGT ACCCCCGCCG 
    AGTTATTCAC ATGTTGGACG GACTCCGTCT TATGATGGAC AACCACCACC GAGTTATAAT 
    CACAGAAATG GTGGAGCCAG CGGAGCAGGA GGATCATCTG GAAGAAATAG TGCTTTCGTA 
    GCACCACTTT CGAATTCATC ATCAAATGCG TCGAGTAGTC ATATGCAACA ACACTATGCT 
    CATCATAATA TGATGCATAA TGGCAATTTG AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT 
    CCGAAACATT ATCGAAGCTC GTCGGCTAGT GATTTGCAGC ACGATCCAAA CGCCTCATTC 
    TCTCAATCAT CAATGACTGG AAGTGCGAGA AGTTCAGTGT TTGACAAGCT TCCATCTCAC 
    TACCGTCACT CGACACGGCC TACAGTAATC CAGCCAACAC GTCCAAGTGC TCAATCTCCA 
    TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG CCGGTCAGCA ATCTGTACCG TCCGACGTCG 
    GCGTCGAATC TGTTTGCACC GCCCAGAAAT TCGGGCGGTC TCGTCGGATA TTCGAGTAAC 
    GCGGCACGGG AGTCGACACA GGATATTCAT CGAGCGCTAA ATGACCTGGA ACTCAACTCA 
    CCGCTCGCCA GATCCACGCC GAATGAGGAG TACCGTATCC CGCCGCCTAG AGTACCTACA 
    GTAACCTCCA CCTCCTCCAA CTATGCCCCT CTTCATTCTA TTCGACCGGC AAGCATGTAT 
    GGATATCAGA GCCCGAGCTC TTCTACTACA GTAATCCCGG CTCCAAAAGG AGTGGCTTCT 
    TCCTCTAATA ACACTTCTCC CCGAGTGAAT GTGACGTTGG GTGGAGCATT GAGCTCATCG 
    AGATCTCATA ATAGTCTTTC ACAGGAGCCA GCCATTGTTA TCAGGCCTGT CGACCTGAAT 
    ATGCCGAAAG CGACACCACC GGCGTCGATT CGTGGAGCTG TGCCGACGGC GATTACACAG 
    GCTCTTCAAC TTCAACCAAA CGGCGGTGGA GCAGTACTTC GTCAGGTTCG GCCAGCATCC 
    GGGCTCCGCG AGCGACTTCA GGACGCTGAT GTTCGGAATT TGGAGAAAAC GAACACAGCA 
    CTGATGAGCT ATGAAGCAGT GAATGAGGAG CTCGATCGAC TCGACGCTAA GCAGAATACG 
    ATGACAATTG ACGAGCAACG GAGATATCGA GAGCTACTGA ACGTTGTCGC CGAGCAGAGC 
    CGAGCAAGGA AGCTGCTCTC GGAGATCAGA TCACCACCTA CATCTTATCA AGAACGTGAA 
    ATTCCGATTC ATGTGCAAAA AGAGCAGCAG CAGCACCGGC GGGAAACTGT GATTGATGAT 
    ATTCCACACT CGAGCTCAAG TGTTGAGAAT GTGTCACCTG TGAGCCCGCC GGAAAGTCGA 
    AAAGTTCAAT TTATTGATGA AGTTGTGCAG TATCAGACGC CGACCAGCGG AGCCAATCAC 
    GCTTTCTTTG ATGATGTGCC GGTTTTGGAT TCGCCTGGGA TCGTCGGCAC CAACGAAATC 
    TACCGTGATC CCCGCCAACG TCGGCTCAAC GAGCTCCAAG ACCGCAATCG CAGCTCGGAA 
    AATGGAGCGG CGGACGGCGC GAAGCTCGGC TTCCGCGACA AGCAGCGCCT ATTCGCACGG 
    CAAATCGGCG AGGACACGGC ACCACGCCAA CGAATGAATG AGAGCAGTGC TCAGCGGCTC 
    ATTGAGACGG ACATTGCCGC CACCCCGCAA AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306529.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293458.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa337252
    Clone ID Related Accession (Same CDS sequence) NM_001306529.2 , NM_001306529.1
    Accession Version NM_001306529.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGTCGAG TCAACTCGGA ATCTCGTCTC CACAAAACAG AAACCACATT TGGAGTCGGT 
    TCTTCTTCTC CAACCATCCC ATCATCTTAT GCACTTCCCA GACACCAACA AACTTCTCGT 
    TATATCTATC ATCGCCGAGC AGCATCCACA GATGCTGAGC CCCTCCTCGC ATCGTTTGAT 
    GCACCTGTGC CCCGACCCCG TCGGGTCACT TTTTCGCAGA CATCTGCGGC AAAGCCACCG 
    CCGCCAGTTC CGATACCCGT CACGAGGTTT CAGACGGCTG CACCTGCTGC GAATTTCAGT 
    GCTAGACAGC TTAAACATCT CATGTCCGGC TCATCAGTCT CATCATCACG GCAGGAGGAT 
    CTTATGATGC ATTCCAATAA TAACATCCTG CCACCGTACT CTACAAAATC CGCGCAGGAA 
    CCCCTCCAAC TGCCGCTCTC AATTCAAATC GGCGACTACA ATGTATGGGA TTTCCTGGCC 
    GAAGTGATGT CGAATCGTGC GGCAACCTCA TCGGAAATGT CCACATTCCG CCTATCGCCT 
    GCCTACTCTC TCTACCTGGC ACTTCGATCA TTCCACACAC ACAATAAACC GTACTCTGTG 
    CAATTCTTCT CACGGATCGA GTCGAATTTC AAGCAAATCG CTCAGGAATG CTCGTCGAAG 
    GATGATCTGA TCTTTTGGCT TGCCAACGCA TCGGAATTCA ATCATCTTGT CGAGCGGGAC 
    ACTGAGCTGA AAGCGTCGAG ATATGGCAAA ATATCTGTGG AAATTGAGAG AATATTCCGG 
    CGATTGGTTG CAGTGCTTCA AAGTGTCATC CGTCCGGCGG CGCGGGCTCT GTTGGACATT 
    CATCTGAATG ATCACGACGC GACCGGCGAT GTGATTCATC TTCTCGACAC CACGATGCGC 
    CAGGCGAGAA TTTGTGGTCT CAACGCGGCG CTCACAATTC AACTGTGCGG GCATCTCCTC 
    CACATGGTCA ATGCCTTTGT GTTCAACAGC TTGGTCTCAG TCAACCTTCC AGCTGCCCAG 
    TTGAATACTC GCCTCGGAAA GTGTCTTCAG TATCGGATAG AGTCGATTCA CAGATTCTGC 
    GAACGAATGG GCGTCGAGAT GGCCGCCGAG TGCCATTTGG ATAGATCCCG TCAGGCCGCT 
    AACCTTCTCG CCACGCAGAA GAATGATGTT GCCGCGTTGG GATCGACGTG CTATAAGCTG 
    AACAGCCTCC AAGTGGTTCA TATCCTGTCG GGATTCGAAC CAGACGACGG CGAGATTCCA 
    TGTGACGATG ACGTCGTTGA TCGGATCTGT AGACTCGCTG AAAAACAGGC GGATAAGCTG 
    ACGTTGGAGG ATGGGCAGCG CGTGACACTG GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC 
    CTGATCCCTC AGGATGGTTA CTTTGTCGAG CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG 
    CAGTACTTGC AGCAACTTCA GCATAGGCGC CTCTGTCATA CGGTGTCGCT TACACCGGCA 
    CCGCCGGCTG CGATGACCCA TTCGATGCAT GCGATGCATA TTGGAGCAAG CGATGCTCAT 
    TATCATCAGC ATCAGCATCA GCAGCAGCAG AACCCGATTG TGAGAAGTGT GTCGCAGACT 
    ACACTGACCA GTATTCCGAC TACACAATTT GGCGACAGTT TTGGAAGTGC CGTGTCGGAT 
    ACGATATCGA AGGTGACACT GCGGAAGAAT GGAGGAGGCA TCGGATTGAG TATTGTCGCT 
    GCTCAGGGTG TTGGTGATCG TCAAATGGGA ATTTATGTGA AGAAGGTTGT CGAAGGAACA 
    CCGGCTGCAC ACGATGGACG ACTCGAGACT GGTGATCAGT TATTGAGTGT TAACGGACAT 
    TCGTTAATTG GAATTTCACA GGAAGACGCC GCCCGTCTCA TGACCCAAAG TGGAAACGAG 
    GTGCATTTCG ACGTCCGGAA AAGAGCCGCT CACCGTAACG GGCTCAGCAA TTGGCTCGGA 
    TCCCAAACAC CGACAACTTC AAATTTCATT GCACCATCTC CATCTACAGT ACCCCCGCCG 
    AGTTATTCAC ATGTTGGACG GACTCCGTCT TATGATGGAC AACCACCACC GAGTTATAAT 
    CACAGAAATG GTGGAGCCAG CGGAGCAGGA GGATCATCTG GAAGAAATAG TGCTTTCGTA 
    GCACCACTTT CGAATTCATC ATCAAATGCG TCGAGTAGTC ATATGCAACA ACACTATGCT 
    CATCATAATA TGATGCATAA TGGCAATTTG AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT 
    CCGAAACATT ATCGAAGCTC GTCGGCTAGT GATTTGCAGC ACGATCCAAA CGCCTCATTC 
    TCTCAATCAT CAATGACTGG AAGTGCGAGA AGTTCAGTGT TTGACAAGCT TCCATCTCAC 
    TACCGTCACT CGACACGGCC TACAGTAATC CAGCCAACAC GTCCAAGTGC TCAATCTCCA 
    TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG CCGGTCAGCA ATCTGTACCG TCCGACGTCG 
    GCGTCGAATC TGTTTGCACC GCCCAGAAAT TCGGGCGGTC TCGTCGGATA TTCGAGTAAC 
    GCGGCACGGG AGTCGACACA GGATATTCAT CGAGCGCTAA ATGACCTGGA ACTCAACTCA 
    CCGCTCGCCA GATCCACGCC GAATGAGGAG TACCGTATCC CGCCGCCTAG AGTACCTACA 
    GTAACCTCCA CCTCCTCCAA CTATGCCCCT CTTCATTCTA TTCGACCGGC AAGCATGTAT 
    GGATATCAGA GCCCGAGCTC TTCTACTACA GTAATCCCGG CTCCAAAAGG AGTGGCTTCT 
    TCCTCTAATA ACACTTCTCC CCGAGTGAAT GTGACGTTGG GTGGAGCATT GAGCTCATCG 
    AGATCTCATA ATAGTCTTTC ACAGGAGCCA GCCATTGTTA TCAGGCCTGT CGACCTGAAT 
    ATGCCGAAAG CGACACCACC GGCGTCGATT CGTGGAGCTG TGCCGACGGC GATTACACAG 
    GCTCTTCAAC TTCAACCAAA CGGCGGTGGA GCAGTACTTC GTCAGGTTCG GCCAGCATCC 
    GGGCTCCGCG AGCGACTTCA GGACGCTGAT GTTCGGAATT TGGAGAAAAC GAACACAGCA 
    CTGATGAGCT ATGAAGCAGT GAATGAGGAG CTCGATCGAC TCGACGCTAA GCAGAATACG 
    ATGACAATTG ACGAGCAACG GAGATATCGA GAGCTACTGA ACGTTGTCGC CGAGCAGAGC 
    CGAGCAAGGA AGCTGCTCTC GGAGATCAGA TCACCACCTA CATCTTATCA AGAACGTGAA 
    ATTCCGATTC ATGTGCAAAA AGAGCAGCAG CAGCACCGGC GGGAAACTGT GATTGATGAT 
    ATTCCACACT CGAGCTCAAG TGTTGAGAAT GTGTCACCTG TGAGCCCGCC GGAAAGTCGA 
    AAAGTTCAAT TTATTGATGA AGTTGTGCAG TATCAGACGC CGACCAGCGG AGCCAATCAC 
    GCTTTCTTTG ATGATGTGCC GGTTTTGGAT TCGCCTGGGA TCGTCGGCAC CAACGAAATC 
    TACCGTGATC CCCGCCAACG TCGGCTCAAC GAGCTCCAAG ACCGCAATCG CAGCTCGGAA 
    AATGGAGCGG CGGACGGCGC GAAGCTCGGC TTCCGCGACA AGCAGCGCCT ATTCGCACGG 
    CAAATCGGCG AGGACACGGC ACCACGCCAA CGAATGAATG AGAGCAGTGC TCAGCGGCTC 
    ATTGAGACGG ACATTGCCGC CACCCCGCAA AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293458.1
    CDS1..3759
    Translation

    Target ORF information:

    RefSeq Version NM_001306529.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGTCGAG TCAACTCGGA ATCTCGTCTC CACAAAACAG AAACCACATT TGGAGTCGGT 
    TCTTCTTCTC CAACCATCCC ATCATCTTAT GCACTTCCCA GACACCAACA AACTTCTCGT 
    TATATCTATC ATCGCCGAGC AGCATCCACA GATGCTGAGC CCCTCCTCGC ATCGTTTGAT 
    GCACCTGTGC CCCGACCCCG TCGGGTCACT TTTTCGCAGA CATCTGCGGC AAAGCCACCG 
    CCGCCAGTTC CGATACCCGT CACGAGGTTT CAGACGGCTG CACCTGCTGC GAATTTCAGT 
    GCTAGACAGC TTAAACATCT CATGTCCGGC TCATCAGTCT CATCATCACG GCAGGAGGAT 
    CTTATGATGC ATTCCAATAA TAACATCCTG CCACCGTACT CTACAAAATC CGCGCAGGAA 
    CCCCTCCAAC TGCCGCTCTC AATTCAAATC GGCGACTACA ATGTATGGGA TTTCCTGGCC 
    GAAGTGATGT CGAATCGTGC GGCAACCTCA TCGGAAATGT CCACATTCCG CCTATCGCCT 
    GCCTACTCTC TCTACCTGGC ACTTCGATCA TTCCACACAC ACAATAAACC GTACTCTGTG 
    CAATTCTTCT CACGGATCGA GTCGAATTTC AAGCAAATCG CTCAGGAATG CTCGTCGAAG 
    GATGATCTGA TCTTTTGGCT TGCCAACGCA TCGGAATTCA ATCATCTTGT CGAGCGGGAC 
    ACTGAGCTGA AAGCGTCGAG ATATGGCAAA ATATCTGTGG AAATTGAGAG AATATTCCGG 
    CGATTGGTTG CAGTGCTTCA AAGTGTCATC CGTCCGGCGG CGCGGGCTCT GTTGGACATT 
    CATCTGAATG ATCACGACGC GACCGGCGAT GTGATTCATC TTCTCGACAC CACGATGCGC 
    CAGGCGAGAA TTTGTGGTCT CAACGCGGCG CTCACAATTC AACTGTGCGG GCATCTCCTC 
    CACATGGTCA ATGCCTTTGT GTTCAACAGC TTGGTCTCAG TCAACCTTCC AGCTGCCCAG 
    TTGAATACTC GCCTCGGAAA GTGTCTTCAG TATCGGATAG AGTCGATTCA CAGATTCTGC 
    GAACGAATGG GCGTCGAGAT GGCCGCCGAG TGCCATTTGG ATAGATCCCG TCAGGCCGCT 
    AACCTTCTCG CCACGCAGAA GAATGATGTT GCCGCGTTGG GATCGACGTG CTATAAGCTG 
    AACAGCCTCC AAGTGGTTCA TATCCTGTCG GGATTCGAAC CAGACGACGG CGAGATTCCA 
    TGTGACGATG ACGTCGTTGA TCGGATCTGT AGACTCGCTG AAAAACAGGC GGATAAGCTG 
    ACGTTGGAGG ATGGGCAGCG CGTGACACTG GAGGAGTCCG ACGAGCTGAA CCTTCCGTTC 
    CTGATCCCTC AGGATGGTTA CTTTGTCGAG CAGTTCCGAA CGGTGCCAGA TGGTCTTTGG 
    CAGTACTTGC AGCAACTTCA GCATAGGCGC CTCTGTCATA CGGTGTCGCT TACACCGGCA 
    CCGCCGGCTG CGATGACCCA TTCGATGCAT GCGATGCATA TTGGAGCAAG CGATGCTCAT 
    TATCATCAGC ATCAGCATCA GCAGCAGCAG AACCCGATTG TGAGAAGTGT GTCGCAGACT 
    ACACTGACCA GTATTCCGAC TACACAATTT GGCGACAGTT TTGGAAGTGC CGTGTCGGAT 
    ACGATATCGA AGGTGACACT GCGGAAGAAT GGAGGAGGCA TCGGATTGAG TATTGTCGCT 
    GCTCAGGGTG TTGGTGATCG TCAAATGGGA ATTTATGTGA AGAAGGTTGT CGAAGGAACA 
    CCGGCTGCAC ACGATGGACG ACTCGAGACT GGTGATCAGT TATTGAGTGT TAACGGACAT 
    TCGTTAATTG GAATTTCACA GGAAGACGCC GCCCGTCTCA TGACCCAAAG TGGAAACGAG 
    GTGCATTTCG ACGTCCGGAA AAGAGCCGCT CACCGTAACG GGCTCAGCAA TTGGCTCGGA 
    TCCCAAACAC CGACAACTTC AAATTTCATT GCACCATCTC CATCTACAGT ACCCCCGCCG 
    AGTTATTCAC ATGTTGGACG GACTCCGTCT TATGATGGAC AACCACCACC GAGTTATAAT 
    CACAGAAATG GTGGAGCCAG CGGAGCAGGA GGATCATCTG GAAGAAATAG TGCTTTCGTA 
    GCACCACTTT CGAATTCATC ATCAAATGCG TCGAGTAGTC ATATGCAACA ACACTATGCT 
    CATCATAATA TGATGCATAA TGGCAATTTG AGTGCTGCTC CGGGAGCTCC CGCGGGGGAT 
    CCGAAACATT ATCGAAGCTC GTCGGCTAGT GATTTGCAGC ACGATCCAAA CGCCTCATTC 
    TCTCAATCAT CAATGACTGG AAGTGCGAGA AGTTCAGTGT TTGACAAGCT TCCATCTCAC 
    TACCGTCACT CGACACGGCC TACAGTAATC CAGCCAACAC GTCCAAGTGC TCAATCTCCA 
    TCGGCTCTTC GAAAAGCGGC CGCCGCCTCG CCGGTCAGCA ATCTGTACCG TCCGACGTCG 
    GCGTCGAATC TGTTTGCACC GCCCAGAAAT TCGGGCGGTC TCGTCGGATA TTCGAGTAAC 
    GCGGCACGGG AGTCGACACA GGATATTCAT CGAGCGCTAA ATGACCTGGA ACTCAACTCA 
    CCGCTCGCCA GATCCACGCC GAATGAGGAG TACCGTATCC CGCCGCCTAG AGTACCTACA 
    GTAACCTCCA CCTCCTCCAA CTATGCCCCT CTTCATTCTA TTCGACCGGC AAGCATGTAT 
    GGATATCAGA GCCCGAGCTC TTCTACTACA GTAATCCCGG CTCCAAAAGG AGTGGCTTCT 
    TCCTCTAATA ACACTTCTCC CCGAGTGAAT GTGACGTTGG GTGGAGCATT GAGCTCATCG 
    AGATCTCATA ATAGTCTTTC ACAGGAGCCA GCCATTGTTA TCAGGCCTGT CGACCTGAAT 
    ATGCCGAAAG CGACACCACC GGCGTCGATT CGTGGAGCTG TGCCGACGGC GATTACACAG 
    GCTCTTCAAC TTCAACCAAA CGGCGGTGGA GCAGTACTTC GTCAGGTTCG GCCAGCATCC 
    GGGCTCCGCG AGCGACTTCA GGACGCTGAT GTTCGGAATT TGGAGAAAAC GAACACAGCA 
    CTGATGAGCT ATGAAGCAGT GAATGAGGAG CTCGATCGAC TCGACGCTAA GCAGAATACG 
    ATGACAATTG ACGAGCAACG GAGATATCGA GAGCTACTGA ACGTTGTCGC CGAGCAGAGC 
    CGAGCAAGGA AGCTGCTCTC GGAGATCAGA TCACCACCTA CATCTTATCA AGAACGTGAA 
    ATTCCGATTC ATGTGCAAAA AGAGCAGCAG CAGCACCGGC GGGAAACTGT GATTGATGAT 
    ATTCCACACT CGAGCTCAAG TGTTGAGAAT GTGTCACCTG TGAGCCCGCC GGAAAGTCGA 
    AAAGTTCAAT TTATTGATGA AGTTGTGCAG TATCAGACGC CGACCAGCGG AGCCAATCAC 
    GCTTTCTTTG ATGATGTGCC GGTTTTGGAT TCGCCTGGGA TCGTCGGCAC CAACGAAATC 
    TACCGTGATC CCCGCCAACG TCGGCTCAAC GAGCTCCAAG ACCGCAATCG CAGCTCGGAA 
    AATGGAGCGG CGGACGGCGC GAAGCTCGGC TTCCGCGACA AGCAGCGCCT ATTCGCACGG 
    CAAATCGGCG AGGACACGGC ACCACGCCAA CGAATGAATG AGAGCAGTGC TCAGCGGCTC 
    ATTGAGACGG ACATTGCCGC CACCCCGCAA AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001356800.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001343732.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa529735
    Clone ID Related Accession (Same CDS sequence) NM_001356800.1 , NM_001356800.2
    Accession Version NM_001356800.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCCCTTAC ACCGTCACGA CGACGACTCG CGGAGCAGCA GTTGCTCGAT CGACACGATC 
    GACGAGCTCT GCGAGCTACA ATACGAGTCG GAGCTGCAGC AGCTTCAGAA GCAGAAGGCT 
    CGAGCCGAAG AACTGCAGAG GAAGCGGAGC AACTCGTTTG GCAAGCGGCT CACGCGGCTT 
    CTCTCGTTTG AGCCAAGTTT TGTCGTCGAG AAGCGCAAGA GCCTGCCGGC GGCGACGGCG 
    GAGGATGAGA GATATCCTCC CCGCGCGACG AGGATTCTTA TGAATTCGTT GAGGAAACTC 
    ACGCGGAGCA GGTCTTCGGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001343732.1
    CDS1..4497
    Translation

    Target ORF information:

    RefSeq Version NM_001356800.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001356800.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCCCTTAC ACCGTCACGA CGACGACTCG CGGAGCAGCA GTTGCTCGAT CGACACGATC 
    GACGAGCTCT GCGAGCTACA ATACGAGTCG GAGCTGCAGC AGCTTCAGAA GCAGAAGGCT 
    CGAGCCGAAG AACTGCAGAG GAAGCGGAGC AACTCGTTTG GCAAGCGGCT CACGCGGCTT 
    CTCTCGTTTG AGCCAAGTTT TGTCGTCGAG AAGCGCAAGA GCCTGCCGGC GGCGACGGCG 
    GAGGATGAGA GATATCCTCC CCGCGCGACG AGGATTCTTA TGAATTCGTT GAGGAAACTC 
    ACGCGGAGCA GGTCTTCGGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001356800.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001343732.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa529735
    Clone ID Related Accession (Same CDS sequence) NM_001356800.1 , NM_001356800.2
    Accession Version NM_001356800.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001356800.1. COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCCCTTAC ACCGTCACGA CGACGACTCG CGGAGCAGCA GTTGCTCGAT CGACACGATC 
    GACGAGCTCT GCGAGCTACA ATACGAGTCG GAGCTGCAGC AGCTTCAGAA GCAGAAGGCT 
    CGAGCCGAAG AACTGCAGAG GAAGCGGAGC AACTCGTTTG GCAAGCGGCT CACGCGGCTT 
    CTCTCGTTTG AGCCAAGTTT TGTCGTCGAG AAGCGCAAGA GCCTGCCGGC GGCGACGGCG 
    GAGGATGAGA GATATCCTCC CCGCGCGACG AGGATTCTTA TGAATTCGTT GAGGAAACTC 
    ACGCGGAGCA GGTCTTCGGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001343732.1
    CDS1..4497
    Translation

    Target ORF information:

    RefSeq Version NM_001356800.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001356800.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCCCTTAC ACCGTCACGA CGACGACTCG CGGAGCAGCA GTTGCTCGAT CGACACGATC 
    GACGAGCTCT GCGAGCTACA ATACGAGTCG GAGCTGCAGC AGCTTCAGAA GCAGAAGGCT 
    CGAGCCGAAG AACTGCAGAG GAAGCGGAGC AACTCGTTTG GCAAGCGGCT CACGCGGCTT 
    CTCTCGTTTG AGCCAAGTTT TGTCGTCGAG AAGCGCAAGA GCCTGCCGGC GGCGACGGCG 
    GAGGATGAGA GATATCCTCC CCGCGCGACG AGGATTCTTA TGAATTCGTT GAGGAAACTC 
    ACGCGGAGCA GGTCTTCGGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001026489.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021660.2 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.
    Price & Availability↑
    CloneID OCa335223
    Clone ID Related Accession (Same CDS sequence) NM_001026489.5 , NM_001026489.4
    Accession Version NM_001026489.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001026489.4. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTTT CAGTACAAGT AAACGGAGGA CGAGAAGAAG CGCAATCTCA AGATGATATC 
    TATAAACAAG TACCATTTAA TACATTCACC CGAACTATAT CTAATCCAGA AGCTGTTATG 
    AGAAAACGGA GGGAAAAGAA ATTGGAGACG AAATTGAAGG AAATGATGTT TGGCGGAAGT 
    CTCAAAGTAT ACGGTGGAGA AATTGTGCCA ACTCGTCCAT ATGTCTCAAT ATTGGCTGAA 
    ATCAACGAAA ATGCTGATAG AATTTTGGGT GCTGCACTGG AAAAGTATGG ATTGGAACAT 
    TCGAAAGATG ATTTTATTCT TGTTGAGGTA TCCAACGACG ATGACCGCAA AAGTATGTCA 
    GACCTCCGTG AAATCGACGG ACGGCCCATT CCACCAACAG AGTGCCCGCT TTTCGAGATG 
    ACTGCTCGAA GTGGAAACGG CGAGAATGGA TTCGATTCGT TTTTGGCGAT CAAGCGGAAA 
    CCGCATGATT ATCAAGTCCC CTCCACCTCA GTTGTTGCTC CATCATCAGT CACCCTACCA 
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    CTAAATGACC TGGAACTCAA CTCACCGCTC GCCAGATCCA CGCCGAATGA GGAGTACCGT 
    ATCCCGCCGC CTAGAGTACC TACAGTAACC TCCACCTCCT CCAACTATGC CCCTCTTCAT 
    TCTATTCGAC CGGCAAGCAT GTATGGATAT CAGAGCCCGA GCTCTTCTAC TACAGTAATC 
    CCGGCTCCAA AAGGAGTGGC TTCTTCCTCT AATAACACTT CTCCCCGAGT GAATGTGACG 
    TTGGGTGGAG CATTGAGCTC ATCGAGATCT CATAATAGTC TTTCACAGGA GCCAGCCATT 
    GTTATCAGGC CTGTCGACCT GAATATGCCG AAAGCGACAC CACCGGCGTC GATTCGTGGA 
    GCTGTGCCGA CGGCGATTAC ACAGGCTCTT CAACTTCAAC CAAACGGCGG TGGAGCAGTA 
    CTTCGTCAGG TTCGGCCAGC ATCCGGGCTC CGCGAGCGAC TTCAGGACGC TGATGTTCGG 
    AATTTGGAGA AAACGAACAC AGCACTGATG AGCTATGAAG CAGTGAATGA GGAGCTCGAT 
    CGACTCGACG CTAAGCAGAA TACGATGACA ATTGACGAGC AACGGAGATA TCGAGAGCTA 
    CTGAACGTTG TCGCCGAGCA GAGCCGAGCA AGGAAGCTGC TCTCGGAGAT CAGATCACCA 
    CCTACATCTT ATCAAGAACG TGAAATTCCG ATTCATGTGC AAAAAGAGCA GCAGCAGCAC 
    CGGCGGGAAA CTGTGATTGA TGATATTCCA CACTCGAGCT CAAGTGTTGA GAATGTGTCA 
    CCTGTGAGCC CGCCGGAAAG TCGAAAAGTT CAATTTATTG ATGAAGTTGT GCAGTATCAG 
    ACGCCGACCA GCGGAGCCAA TCACGCTTTC TTTGATGATG TGCCGGTTTT GGATTCGCCT 
    GGGATCGTCG GCACCAACGA AATCTACCGT GATCCCCGCC AACGTCGGCT CAACGAGCTC 
    CAAGACCGCA ATCGCAGCTC GGAAAATGGA GCGGCGGACG GCGCGAAGCT CGGCTTCCGC 
    GACAAGCAGC GCCTATTCGC ACGGCAAATC GGCGAGGACA CGGCACCACG CCAACGAATG 
    AATGAGAGCA GTGCTCAGCG GCTCATTGAG ACGGACATTG CCGCCACCCC GCAAAAGCCA 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001021660.2
    CDS30..5012
    Translation

    Target ORF information:

    RefSeq Version NM_001026489.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001026489.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTTT CAGTACAAGT AAACGGAGGA CGAGAAGAAG CGCAATCTCA AGATGATATC 
    TATAAACAAG TACCATTTAA TACATTCACC CGAACTATAT CTAATCCAGA AGCTGTTATG 
    AGAAAACGGA GGGAAAAGAA ATTGGAGACG AAATTGAAGG AAATGATGTT TGGCGGAAGT 
    CTCAAAGTAT ACGGTGGAGA AATTGTGCCA ACTCGTCCAT ATGTCTCAAT ATTGGCTGAA 
    ATCAACGAAA ATGCTGATAG AATTTTGGGT GCTGCACTGG AAAAGTATGG ATTGGAACAT 
    TCGAAAGATG ATTTTATTCT TGTTGAGGTA TCCAACGACG ATGACCGCAA AAGTATGTCA 
    GACCTCCGTG AAATCGACGG ACGGCCCATT CCACCAACAG AGTGCCCGCT TTTCGAGATG 
    ACTGCTCGAA GTGGAAACGG CGAGAATGGA TTCGATTCGT TTTTGGCGAT CAAGCGGAAA 
    CCGCATGATT ATCAAGTCCC CTCCACCTCA GTTGTTGCTC CATCATCAGT CACCCTACCA 
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    CTAAATGACC TGGAACTCAA CTCACCGCTC GCCAGATCCA CGCCGAATGA GGAGTACCGT 
    ATCCCGCCGC CTAGAGTACC TACAGTAACC TCCACCTCCT CCAACTATGC CCCTCTTCAT 
    TCTATTCGAC CGGCAAGCAT GTATGGATAT CAGAGCCCGA GCTCTTCTAC TACAGTAATC 
    CCGGCTCCAA AAGGAGTGGC TTCTTCCTCT AATAACACTT CTCCCCGAGT GAATGTGACG 
    TTGGGTGGAG CATTGAGCTC ATCGAGATCT CATAATAGTC TTTCACAGGA GCCAGCCATT 
    GTTATCAGGC CTGTCGACCT GAATATGCCG AAAGCGACAC CACCGGCGTC GATTCGTGGA 
    GCTGTGCCGA CGGCGATTAC ACAGGCTCTT CAACTTCAAC CAAACGGCGG TGGAGCAGTA 
    CTTCGTCAGG TTCGGCCAGC ATCCGGGCTC CGCGAGCGAC TTCAGGACGC TGATGTTCGG 
    AATTTGGAGA AAACGAACAC AGCACTGATG AGCTATGAAG CAGTGAATGA GGAGCTCGAT 
    CGACTCGACG CTAAGCAGAA TACGATGACA ATTGACGAGC AACGGAGATA TCGAGAGCTA 
    CTGAACGTTG TCGCCGAGCA GAGCCGAGCA AGGAAGCTGC TCTCGGAGAT CAGATCACCA 
    CCTACATCTT ATCAAGAACG TGAAATTCCG ATTCATGTGC AAAAAGAGCA GCAGCAGCAC 
    CGGCGGGAAA CTGTGATTGA TGATATTCCA CACTCGAGCT CAAGTGTTGA GAATGTGTCA 
    CCTGTGAGCC CGCCGGAAAG TCGAAAAGTT CAATTTATTG ATGAAGTTGT GCAGTATCAG 
    ACGCCGACCA GCGGAGCCAA TCACGCTTTC TTTGATGATG TGCCGGTTTT GGATTCGCCT 
    GGGATCGTCG GCACCAACGA AATCTACCGT GATCCCCGCC AACGTCGGCT CAACGAGCTC 
    CAAGACCGCA ATCGCAGCTC GGAAAATGGA GCGGCGGACG GCGCGAAGCT CGGCTTCCGC 
    GACAAGCAGC GCCTATTCGC ACGGCAAATC GGCGAGGACA CGGCACCACG CCAACGAATG 
    AATGAGAGCA GTGCTCAGCG GCTCATTGAG ACGGACATTG CCGCCACCCC GCAAAAGCCA 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001026489.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021660.2 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa335223
    Clone ID Related Accession (Same CDS sequence) NM_001026489.5 , NM_001026489.4
    Accession Version NM_001026489.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Apr 20, 2015 this sequence version replaced NM_001026489.3. COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTTT CAGTACAAGT AAACGGAGGA CGAGAAGAAG CGCAATCTCA AGATGATATC 
    TATAAACAAG TACCATTTAA TACATTCACC CGAACTATAT CTAATCCAGA AGCTGTTATG 
    AGAAAACGGA GGGAAAAGAA ATTGGAGACG AAATTGAAGG AAATGATGTT TGGCGGAAGT 
    CTCAAAGTAT ACGGTGGAGA AATTGTGCCA ACTCGTCCAT ATGTCTCAAT ATTGGCTGAA 
    ATCAACGAAA ATGCTGATAG AATTTTGGGT GCTGCACTGG AAAAGTATGG ATTGGAACAT 
    TCGAAAGATG ATTTTATTCT TGTTGAGGTA TCCAACGACG ATGACCGCAA AAGTATGTCA 
    GACCTCCGTG AAATCGACGG ACGGCCCATT CCACCAACAG AGTGCCCGCT TTTCGAGATG 
    ACTGCTCGAA GTGGAAACGG CGAGAATGGA TTCGATTCGT TTTTGGCGAT CAAGCGGAAA 
    CCGCATGATT ATCAAGTCCC CTCCACCTCA GTTGTTGCTC CATCATCAGT CACCCTACCA 
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    CTAAATGACC TGGAACTCAA CTCACCGCTC GCCAGATCCA CGCCGAATGA GGAGTACCGT 
    ATCCCGCCGC CTAGAGTACC TACAGTAACC TCCACCTCCT CCAACTATGC CCCTCTTCAT 
    TCTATTCGAC CGGCAAGCAT GTATGGATAT CAGAGCCCGA GCTCTTCTAC TACAGTAATC 
    CCGGCTCCAA AAGGAGTGGC TTCTTCCTCT AATAACACTT CTCCCCGAGT GAATGTGACG 
    TTGGGTGGAG CATTGAGCTC ATCGAGATCT CATAATAGTC TTTCACAGGA GCCAGCCATT 
    GTTATCAGGC CTGTCGACCT GAATATGCCG AAAGCGACAC CACCGGCGTC GATTCGTGGA 
    GCTGTGCCGA CGGCGATTAC ACAGGCTCTT CAACTTCAAC CAAACGGCGG TGGAGCAGTA 
    CTTCGTCAGG TTCGGCCAGC ATCCGGGCTC CGCGAGCGAC TTCAGGACGC TGATGTTCGG 
    AATTTGGAGA AAACGAACAC AGCACTGATG AGCTATGAAG CAGTGAATGA GGAGCTCGAT 
    CGACTCGACG CTAAGCAGAA TACGATGACA ATTGACGAGC AACGGAGATA TCGAGAGCTA 
    CTGAACGTTG TCGCCGAGCA GAGCCGAGCA AGGAAGCTGC TCTCGGAGAT CAGATCACCA 
    CCTACATCTT ATCAAGAACG TGAAATTCCG ATTCATGTGC AAAAAGAGCA GCAGCAGCAC 
    CGGCGGGAAA CTGTGATTGA TGATATTCCA CACTCGAGCT CAAGTGTTGA GAATGTGTCA 
    CCTGTGAGCC CGCCGGAAAG TCGAAAAGTT CAATTTATTG ATGAAGTTGT GCAGTATCAG 
    ACGCCGACCA GCGGAGCCAA TCACGCTTTC TTTGATGATG TGCCGGTTTT GGATTCGCCT 
    GGGATCGTCG GCACCAACGA AATCTACCGT GATCCCCGCC AACGTCGGCT CAACGAGCTC 
    CAAGACCGCA ATCGCAGCTC GGAAAATGGA GCGGCGGACG GCGCGAAGCT CGGCTTCCGC 
    GACAAGCAGC GCCTATTCGC ACGGCAAATC GGCGAGGACA CGGCACCACG CCAACGAATG 
    AATGAGAGCA GTGCTCAGCG GCTCATTGAG ACGGACATTG CCGCCACCCC GCAAAAGCCA 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001021660.2
    CDS1..4983
    Translation

    Target ORF information:

    RefSeq Version NM_001026489.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001026489.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTTT CAGTACAAGT AAACGGAGGA CGAGAAGAAG CGCAATCTCA AGATGATATC 
    TATAAACAAG TACCATTTAA TACATTCACC CGAACTATAT CTAATCCAGA AGCTGTTATG 
    AGAAAACGGA GGGAAAAGAA ATTGGAGACG AAATTGAAGG AAATGATGTT TGGCGGAAGT 
    CTCAAAGTAT ACGGTGGAGA AATTGTGCCA ACTCGTCCAT ATGTCTCAAT ATTGGCTGAA 
    ATCAACGAAA ATGCTGATAG AATTTTGGGT GCTGCACTGG AAAAGTATGG ATTGGAACAT 
    TCGAAAGATG ATTTTATTCT TGTTGAGGTA TCCAACGACG ATGACCGCAA AAGTATGTCA 
    GACCTCCGTG AAATCGACGG ACGGCCCATT CCACCAACAG AGTGCCCGCT TTTCGAGATG 
    ACTGCTCGAA GTGGAAACGG CGAGAATGGA TTCGATTCGT TTTTGGCGAT CAAGCGGAAA 
    CCGCATGATT ATCAAGTCCC CTCCACCTCA GTTGTTGCTC CATCATCAGT CACCCTACCA 
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    CTAAATGACC TGGAACTCAA CTCACCGCTC GCCAGATCCA CGCCGAATGA GGAGTACCGT 
    ATCCCGCCGC CTAGAGTACC TACAGTAACC TCCACCTCCT CCAACTATGC CCCTCTTCAT 
    TCTATTCGAC CGGCAAGCAT GTATGGATAT CAGAGCCCGA GCTCTTCTAC TACAGTAATC 
    CCGGCTCCAA AAGGAGTGGC TTCTTCCTCT AATAACACTT CTCCCCGAGT GAATGTGACG 
    TTGGGTGGAG CATTGAGCTC ATCGAGATCT CATAATAGTC TTTCACAGGA GCCAGCCATT 
    GTTATCAGGC CTGTCGACCT GAATATGCCG AAAGCGACAC CACCGGCGTC GATTCGTGGA 
    GCTGTGCCGA CGGCGATTAC ACAGGCTCTT CAACTTCAAC CAAACGGCGG TGGAGCAGTA 
    CTTCGTCAGG TTCGGCCAGC ATCCGGGCTC CGCGAGCGAC TTCAGGACGC TGATGTTCGG 
    AATTTGGAGA AAACGAACAC AGCACTGATG AGCTATGAAG CAGTGAATGA GGAGCTCGAT 
    CGACTCGACG CTAAGCAGAA TACGATGACA ATTGACGAGC AACGGAGATA TCGAGAGCTA 
    CTGAACGTTG TCGCCGAGCA GAGCCGAGCA AGGAAGCTGC TCTCGGAGAT CAGATCACCA 
    CCTACATCTT ATCAAGAACG TGAAATTCCG ATTCATGTGC AAAAAGAGCA GCAGCAGCAC 
    CGGCGGGAAA CTGTGATTGA TGATATTCCA CACTCGAGCT CAAGTGTTGA GAATGTGTCA 
    CCTGTGAGCC CGCCGGAAAG TCGAAAAGTT CAATTTATTG ATGAAGTTGT GCAGTATCAG 
    ACGCCGACCA GCGGAGCCAA TCACGCTTTC TTTGATGATG TGCCGGTTTT GGATTCGCCT 
    GGGATCGTCG GCACCAACGA AATCTACCGT GATCCCCGCC AACGTCGGCT CAACGAGCTC 
    CAAGACCGCA ATCGCAGCTC GGAAAATGGA GCGGCGGACG GCGCGAAGCT CGGCTTCCGC 
    GACAAGCAGC GCCTATTCGC ACGGCAAATC GGCGAGGACA CGGCACCACG CCAACGAATG 
    AATGAGAGCA GTGCTCAGCG GCTCATTGAG ACGGACATTG CCGCCACCCC GCAAAAGCCA 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001026490.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021661.2 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa335224
    Clone ID Related Accession (Same CDS sequence) NM_001026490.4 , NM_001026490.5
    Accession Version NM_001026490.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4689bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Apr 20, 2015 this sequence version replaced NM_001026490.3. COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTAC AAGTAAACGG AGGACGAGAA GAAGCGCAAT CTCAAGATGA TATCTATAAA 
    CAAGTACCAT TTAATACATT CACCCGAACT ATATCTAATC CAGAAGCTGT TATGAGAAAA 
    CGGAGGGAAA AGAAATTGGA GACGAAATTG AAGGAAATGA TGTTTGGCGG AAGTCTCAAA 
    GTATACGGTG GAGAAATTGT GCCAACTCGT CCATATGTCT CAATATTGGC TGAAATCAAC 
    GAAAATGCTG ATAGAATTTT GGGTGCTGCA CTGGAAAAGT ATGGATTGGA ACATTCGAAA 
    GATGATTTTA TTCTTGTTGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGGAGCCA 
    GCCATTGTTA TCAGGCCTGT CGACCTGAAT ATGCCGAAAG CGACACCACC GGCGTCGATT 
    CGTGGAGCTG TGCCGACGGC GATTACACAG GCTCTTCAAC TTCAACCAAA CGGCGGTGGA 
    GCAGTACTTC GTCAGGTTCG GCCAGCATCC GGGCTCCGCG AGCGACTTCA GGACGCTGAT 
    GTTCGGAATT TGGAGAAAAC GAACACAGCA CTGATGAGCT ATGAAGCAGT GAATGAGGAG 
    CTCGATCGAC TCGACGCTAA GCAGAATACG ATGACAATTG ACGAGCAACG GAGATATCGA 
    GAGCTACTGA ACGTTGTCGC CGAGCAGAGC CGAGCAAGGA AGCTGCTCTC GGAGATCAGA 
    TCACCACCTA CATCTTATCA AGAACGTGAA ATTCCGATTC ATGTGCAAAA AGAGCAGCAG 
    CAGCACCGGC GGGAAACTGT GATTGATGAT ATTCCACACT CGAGCTCAAG TGTTGAGAAT 
    GTGTCACCTG TGAGCCCGCC GGAAAGTCGA AAAGTTCAAT TTATTGATGA AGTTGTGCAG 
    TATCAGACGC CGACCAGCGG AGCCAATCAC GCTTTCTTTG ATGATGTGCC GGTTTTGGAT 
    TCGCCTGGGA TCGTCGGCAC CAACGAAATC TACCGTGATC CCCGCCAACG TCGGCTCAAC 
    GAGCTCCAAG ACCGCAATCG CAGCTCGGAA AATGGAGCGG CGGACGGCGC GAAGCTCGGC 
    TTCCGCGACA AGCAGCGCCT ATTCGCACGG CAAATCGGCG AGGACACGGC ACCACGCCAA 
    CGAATGAATG AGAGCAGTGC TCAGCGGCTC ATTGAGACGG ACATTGCCGC CACCCCGCAA 
    AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001021661.2
    CDS1..4689
    Translation

    Target ORF information:

    RefSeq Version NM_001026490.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001026490.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTAC AAGTAAACGG AGGACGAGAA GAAGCGCAAT CTCAAGATGA TATCTATAAA 
    CAAGTACCAT TTAATACATT CACCCGAACT ATATCTAATC CAGAAGCTGT TATGAGAAAA 
    CGGAGGGAAA AGAAATTGGA GACGAAATTG AAGGAAATGA TGTTTGGCGG AAGTCTCAAA 
    GTATACGGTG GAGAAATTGT GCCAACTCGT CCATATGTCT CAATATTGGC TGAAATCAAC 
    GAAAATGCTG ATAGAATTTT GGGTGCTGCA CTGGAAAAGT ATGGATTGGA ACATTCGAAA 
    GATGATTTTA TTCTTGTTGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGGAGCCA 
    GCCATTGTTA TCAGGCCTGT CGACCTGAAT ATGCCGAAAG CGACACCACC GGCGTCGATT 
    CGTGGAGCTG TGCCGACGGC GATTACACAG GCTCTTCAAC TTCAACCAAA CGGCGGTGGA 
    GCAGTACTTC GTCAGGTTCG GCCAGCATCC GGGCTCCGCG AGCGACTTCA GGACGCTGAT 
    GTTCGGAATT TGGAGAAAAC GAACACAGCA CTGATGAGCT ATGAAGCAGT GAATGAGGAG 
    CTCGATCGAC TCGACGCTAA GCAGAATACG ATGACAATTG ACGAGCAACG GAGATATCGA 
    GAGCTACTGA ACGTTGTCGC CGAGCAGAGC CGAGCAAGGA AGCTGCTCTC GGAGATCAGA 
    TCACCACCTA CATCTTATCA AGAACGTGAA ATTCCGATTC ATGTGCAAAA AGAGCAGCAG 
    CAGCACCGGC GGGAAACTGT GATTGATGAT ATTCCACACT CGAGCTCAAG TGTTGAGAAT 
    GTGTCACCTG TGAGCCCGCC GGAAAGTCGA AAAGTTCAAT TTATTGATGA AGTTGTGCAG 
    TATCAGACGC CGACCAGCGG AGCCAATCAC GCTTTCTTTG ATGATGTGCC GGTTTTGGAT 
    TCGCCTGGGA TCGTCGGCAC CAACGAAATC TACCGTGATC CCCGCCAACG TCGGCTCAAC 
    GAGCTCCAAG ACCGCAATCG CAGCTCGGAA AATGGAGCGG CGGACGGCGC GAAGCTCGGC 
    TTCCGCGACA AGCAGCGCCT ATTCGCACGG CAAATCGGCG AGGACACGGC ACCACGCCAA 
    CGAATGAATG AGAGCAGTGC TCAGCGGCTC ATTGAGACGG ACATTGCCGC CACCCCGCAA 
    AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001026490.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021661.2 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.
    Price & Availability↑
    CloneID OCa335224
    Clone ID Related Accession (Same CDS sequence) NM_001026490.4 , NM_001026490.5
    Accession Version NM_001026490.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4689bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001026490.4. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTAC AAGTAAACGG AGGACGAGAA GAAGCGCAAT CTCAAGATGA TATCTATAAA 
    CAAGTACCAT TTAATACATT CACCCGAACT ATATCTAATC CAGAAGCTGT TATGAGAAAA 
    CGGAGGGAAA AGAAATTGGA GACGAAATTG AAGGAAATGA TGTTTGGCGG AAGTCTCAAA 
    GTATACGGTG GAGAAATTGT GCCAACTCGT CCATATGTCT CAATATTGGC TGAAATCAAC 
    GAAAATGCTG ATAGAATTTT GGGTGCTGCA CTGGAAAAGT ATGGATTGGA ACATTCGAAA 
    GATGATTTTA TTCTTGTTGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGGAGCCA 
    GCCATTGTTA TCAGGCCTGT CGACCTGAAT ATGCCGAAAG CGACACCACC GGCGTCGATT 
    CGTGGAGCTG TGCCGACGGC GATTACACAG GCTCTTCAAC TTCAACCAAA CGGCGGTGGA 
    GCAGTACTTC GTCAGGTTCG GCCAGCATCC GGGCTCCGCG AGCGACTTCA GGACGCTGAT 
    GTTCGGAATT TGGAGAAAAC GAACACAGCA CTGATGAGCT ATGAAGCAGT GAATGAGGAG 
    CTCGATCGAC TCGACGCTAA GCAGAATACG ATGACAATTG ACGAGCAACG GAGATATCGA 
    GAGCTACTGA ACGTTGTCGC CGAGCAGAGC CGAGCAAGGA AGCTGCTCTC GGAGATCAGA 
    TCACCACCTA CATCTTATCA AGAACGTGAA ATTCCGATTC ATGTGCAAAA AGAGCAGCAG 
    CAGCACCGGC GGGAAACTGT GATTGATGAT ATTCCACACT CGAGCTCAAG TGTTGAGAAT 
    GTGTCACCTG TGAGCCCGCC GGAAAGTCGA AAAGTTCAAT TTATTGATGA AGTTGTGCAG 
    TATCAGACGC CGACCAGCGG AGCCAATCAC GCTTTCTTTG ATGATGTGCC GGTTTTGGAT 
    TCGCCTGGGA TCGTCGGCAC CAACGAAATC TACCGTGATC CCCGCCAACG TCGGCTCAAC 
    GAGCTCCAAG ACCGCAATCG CAGCTCGGAA AATGGAGCGG CGGACGGCGC GAAGCTCGGC 
    TTCCGCGACA AGCAGCGCCT ATTCGCACGG CAAATCGGCG AGGACACGGC ACCACGCCAA 
    CGAATGAATG AGAGCAGTGC TCAGCGGCTC ATTGAGACGG ACATTGCCGC CACCCCGCAA 
    AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001021661.2
    CDS30..4718
    Translation

    Target ORF information:

    RefSeq Version NM_001026490.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001026490.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTAC AAGTAAACGG AGGACGAGAA GAAGCGCAAT CTCAAGATGA TATCTATAAA 
    CAAGTACCAT TTAATACATT CACCCGAACT ATATCTAATC CAGAAGCTGT TATGAGAAAA 
    CGGAGGGAAA AGAAATTGGA GACGAAATTG AAGGAAATGA TGTTTGGCGG AAGTCTCAAA 
    GTATACGGTG GAGAAATTGT GCCAACTCGT CCATATGTCT CAATATTGGC TGAAATCAAC 
    GAAAATGCTG ATAGAATTTT GGGTGCTGCA CTGGAAAAGT ATGGATTGGA ACATTCGAAA 
    GATGATTTTA TTCTTGTTGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGGAGCCA 
    GCCATTGTTA TCAGGCCTGT CGACCTGAAT ATGCCGAAAG CGACACCACC GGCGTCGATT 
    CGTGGAGCTG TGCCGACGGC GATTACACAG GCTCTTCAAC TTCAACCAAA CGGCGGTGGA 
    GCAGTACTTC GTCAGGTTCG GCCAGCATCC GGGCTCCGCG AGCGACTTCA GGACGCTGAT 
    GTTCGGAATT TGGAGAAAAC GAACACAGCA CTGATGAGCT ATGAAGCAGT GAATGAGGAG 
    CTCGATCGAC TCGACGCTAA GCAGAATACG ATGACAATTG ACGAGCAACG GAGATATCGA 
    GAGCTACTGA ACGTTGTCGC CGAGCAGAGC CGAGCAAGGA AGCTGCTCTC GGAGATCAGA 
    TCACCACCTA CATCTTATCA AGAACGTGAA ATTCCGATTC ATGTGCAAAA AGAGCAGCAG 
    CAGCACCGGC GGGAAACTGT GATTGATGAT ATTCCACACT CGAGCTCAAG TGTTGAGAAT 
    GTGTCACCTG TGAGCCCGCC GGAAAGTCGA AAAGTTCAAT TTATTGATGA AGTTGTGCAG 
    TATCAGACGC CGACCAGCGG AGCCAATCAC GCTTTCTTTG ATGATGTGCC GGTTTTGGAT 
    TCGCCTGGGA TCGTCGGCAC CAACGAAATC TACCGTGATC CCCGCCAACG TCGGCTCAAC 
    GAGCTCCAAG ACCGCAATCG CAGCTCGGAA AATGGAGCGG CGGACGGCGC GAAGCTCGGC 
    TTCCGCGACA AGCAGCGCCT ATTCGCACGG CAAATCGGCG AGGACACGGC ACCACGCCAA 
    CGAATGAATG AGAGCAGTGC TCAGCGGCTC ATTGAGACGG ACATTGCCGC CACCCCGCAA 
    AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306400.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293329.1 Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa336161
    Clone ID Related Accession (Same CDS sequence) NM_001306400.1 , NM_001306400.2
    Accession Version NM_001306400.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product AFaDin (actin filament binding protein) homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTTT CAGTACAAGT AAACGGAGGA CGAGAAGAAG CGCAATCTCA AGATGATATC 
    TATAAACAAG TACCATTTAA TACATTCACC CGAACTATAT CTAATCCAGA AGCTGTTATG 
    AGAAAACGGA GGGAAAAGAA ATTGGAGACG AAATTGAAGG AAATGATGTT TGGCGGAAGT 
    CTCAAAGTAT ACGGTGGAGA AATTGTGCCA ACTCGTCCAT ATGTCTCAAT ATTGGCTGAA 
    ATCAACGAAA ATGCTGATAG AATTTTGGGT GCTGCACTGG AAAAGTATGG ATTGGAACAT 
    TCGAAAGATG ATTTTATTCT TGTTGAGGTA TCCAACGACG ATGACCGCAA AAGTATGTCA 
    GACCTCCGTG AAATCGACGG ACGGCCCATT CCACCAACAG AGTGCCCGCT TTTCGAGATG 
    ACTGCTCGAA GTGGAAACGG CGAGAATGGA TTCGATTCGT TTTTGGCGAT CAAGCGGAAA 
    CCGCATGATT ATCAAGTCCC CTCCACCTCA GTTGTTGCTC CATCATCAGT CACCCTACCA 
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    GAGCCAGCCA TTGTTATCAG GCCTGTCGAC CTGAATATGC CGAAAGCGAC ACCACCGGCG 
    TCGATTCGTG GAGCTGTGCC GACGGCGATT ACACAGGCTC TTCAACTTCA ACCAAACGGC 
    GGTGGAGCAG TACTTCGTCA GGTTCGGCCA GCATCCGGGC TCCGCGAGCG ACTTCAGGAC 
    GCTGATGTTC GGAATTTGGA GAAAACGAAC ACAGCACTGA TGAGCTATGA AGCAGTGAAT 
    GAGGAGCTCG ATCGACTCGA CGCTAAGCAG AATACGATGA CAATTGACGA GCAACGGAGA 
    TATCGAGAGC TACTGAACGT TGTCGCCGAG CAGAGCCGAG CAAGGAAGCT GCTCTCGGAG 
    ATCAGATCAC CACCTACATC TTATCAAGAA CGTGAAATTC CGATTCATGT GCAAAAAGAG 
    CAGCAGCAGC ACCGGCGGGA AACTGTGATT GATGATATTC CACACTCGAG CTCAAGTGTT 
    GAGAATGTGT CACCTGTGAG CCCGCCGGAA AGTCGAAAAG TTCAATTTAT TGATGAAGTT 
    GTGCAGTATC AGACGCCGAC CAGCGGAGCC AATCACGCTT TCTTTGATGA TGTGCCGGTT 
    TTGGATTCGC CTGGGATCGT CGGCACCAAC GAAATCTACC GTGATCCCCG CCAACGTCGG 
    CTCAACGAGC TCCAAGACCG CAATCGCAGC TCGGAAAATG GAGCGGCGGA CGGCGCGAAG 
    CTCGGCTTCC GCGACAAGCA GCGCCTATTC GCACGGCAAA TCGGCGAGGA CACGGCACCA 
    CGCCAACGAA TGAATGAGAG CAGTGCTCAG CGGCTCATTG AGACGGACAT TGCCGCCACC 
    CCGCAAAAGC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293329.1
    CDS1..4695
    Translation

    Target ORF information:

    RefSeq Version NM_001306400.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans AFaDin (actin filament binding protein) homolog (afd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306400.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTTT CAGTACAAGT AAACGGAGGA CGAGAAGAAG CGCAATCTCA AGATGATATC 
    TATAAACAAG TACCATTTAA TACATTCACC CGAACTATAT CTAATCCAGA AGCTGTTATG 
    AGAAAACGGA GGGAAAAGAA ATTGGAGACG AAATTGAAGG AAATGATGTT TGGCGGAAGT 
    CTCAAAGTAT ACGGTGGAGA AATTGTGCCA ACTCGTCCAT ATGTCTCAAT ATTGGCTGAA 
    ATCAACGAAA ATGCTGATAG AATTTTGGGT GCTGCACTGG AAAAGTATGG ATTGGAACAT 
    TCGAAAGATG ATTTTATTCT TGTTGAGGTA TCCAACGACG ATGACCGCAA AAGTATGTCA 
    GACCTCCGTG AAATCGACGG ACGGCCCATT CCACCAACAG AGTGCCCGCT TTTCGAGATG 
    ACTGCTCGAA GTGGAAACGG CGAGAATGGA TTCGATTCGT TTTTGGCGAT CAAGCGGAAA 
    CCGCATGATT ATCAAGTCCC CTCCACCTCA GTTGTTGCTC CATCATCAGT CACCCTACCA 
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    GAGCCAGCCA TTGTTATCAG GCCTGTCGAC CTGAATATGC CGAAAGCGAC ACCACCGGCG 
    TCGATTCGTG GAGCTGTGCC GACGGCGATT ACACAGGCTC TTCAACTTCA ACCAAACGGC 
    GGTGGAGCAG TACTTCGTCA GGTTCGGCCA GCATCCGGGC TCCGCGAGCG ACTTCAGGAC 
    GCTGATGTTC GGAATTTGGA GAAAACGAAC ACAGCACTGA TGAGCTATGA AGCAGTGAAT 
    GAGGAGCTCG ATCGACTCGA CGCTAAGCAG AATACGATGA CAATTGACGA GCAACGGAGA 
    TATCGAGAGC TACTGAACGT TGTCGCCGAG CAGAGCCGAG CAAGGAAGCT GCTCTCGGAG 
    ATCAGATCAC CACCTACATC TTATCAAGAA CGTGAAATTC CGATTCATGT GCAAAAAGAG 
    CAGCAGCAGC ACCGGCGGGA AACTGTGATT GATGATATTC CACACTCGAG CTCAAGTGTT 
    GAGAATGTGT CACCTGTGAG CCCGCCGGAA AGTCGAAAAG TTCAATTTAT TGATGAAGTT 
    GTGCAGTATC AGACGCCGAC CAGCGGAGCC AATCACGCTT TCTTTGATGA TGTGCCGGTT 
    TTGGATTCGC CTGGGATCGT CGGCACCAAC GAAATCTACC GTGATCCCCG CCAACGTCGG 
    CTCAACGAGC TCCAAGACCG CAATCGCAGC TCGGAAAATG GAGCGGCGGA CGGCGCGAAG 
    CTCGGCTTCC GCGACAAGCA GCGCCTATTC GCACGGCAAA TCGGCGAGGA CACGGCACCA 
    CGCCAACGAA TGAATGAGAG CAGTGCTCAG CGGCTCATTG AGACGGACAT TGCCGCCACC 
    CCGCAAAAGC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_001306400.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293329.1 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.
    Price & Availability↑
    CloneID OCa336161
    Clone ID Related Accession (Same CDS sequence) NM_001306400.1 , NM_001306400.2
    Accession Version NM_001306400.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306400.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTTT CAGTACAAGT AAACGGAGGA CGAGAAGAAG CGCAATCTCA AGATGATATC 
    TATAAACAAG TACCATTTAA TACATTCACC CGAACTATAT CTAATCCAGA AGCTGTTATG 
    AGAAAACGGA GGGAAAAGAA ATTGGAGACG AAATTGAAGG AAATGATGTT TGGCGGAAGT 
    CTCAAAGTAT ACGGTGGAGA AATTGTGCCA ACTCGTCCAT ATGTCTCAAT ATTGGCTGAA 
    ATCAACGAAA ATGCTGATAG AATTTTGGGT GCTGCACTGG AAAAGTATGG ATTGGAACAT 
    TCGAAAGATG ATTTTATTCT TGTTGAGGTA TCCAACGACG ATGACCGCAA AAGTATGTCA 
    GACCTCCGTG AAATCGACGG ACGGCCCATT CCACCAACAG AGTGCCCGCT TTTCGAGATG 
    ACTGCTCGAA GTGGAAACGG CGAGAATGGA TTCGATTCGT TTTTGGCGAT CAAGCGGAAA 
    CCGCATGATT ATCAAGTCCC CTCCACCTCA GTTGTTGCTC CATCATCAGT CACCCTACCA 
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    GAGCCAGCCA TTGTTATCAG GCCTGTCGAC CTGAATATGC CGAAAGCGAC ACCACCGGCG 
    TCGATTCGTG GAGCTGTGCC GACGGCGATT ACACAGGCTC TTCAACTTCA ACCAAACGGC 
    GGTGGAGCAG TACTTCGTCA GGTTCGGCCA GCATCCGGGC TCCGCGAGCG ACTTCAGGAC 
    GCTGATGTTC GGAATTTGGA GAAAACGAAC ACAGCACTGA TGAGCTATGA AGCAGTGAAT 
    GAGGAGCTCG ATCGACTCGA CGCTAAGCAG AATACGATGA CAATTGACGA GCAACGGAGA 
    TATCGAGAGC TACTGAACGT TGTCGCCGAG CAGAGCCGAG CAAGGAAGCT GCTCTCGGAG 
    ATCAGATCAC CACCTACATC TTATCAAGAA CGTGAAATTC CGATTCATGT GCAAAAAGAG 
    CAGCAGCAGC ACCGGCGGGA AACTGTGATT GATGATATTC CACACTCGAG CTCAAGTGTT 
    GAGAATGTGT CACCTGTGAG CCCGCCGGAA AGTCGAAAAG TTCAATTTAT TGATGAAGTT 
    GTGCAGTATC AGACGCCGAC CAGCGGAGCC AATCACGCTT TCTTTGATGA TGTGCCGGTT 
    TTGGATTCGC CTGGGATCGT CGGCACCAAC GAAATCTACC GTGATCCCCG CCAACGTCGG 
    CTCAACGAGC TCCAAGACCG CAATCGCAGC TCGGAAAATG GAGCGGCGGA CGGCGCGAAG 
    CTCGGCTTCC GCGACAAGCA GCGCCTATTC GCACGGCAAA TCGGCGAGGA CACGGCACCA 
    CGCCAACGAA TGAATGAGAG CAGTGCTCAG CGGCTCATTG AGACGGACAT TGCCGCCACC 
    CCGCAAAAGC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293329.1
    CDS30..4724
    Translation

    Target ORF information:

    RefSeq Version NM_001306400.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306400.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTTT CAGTACAAGT AAACGGAGGA CGAGAAGAAG CGCAATCTCA AGATGATATC 
    TATAAACAAG TACCATTTAA TACATTCACC CGAACTATAT CTAATCCAGA AGCTGTTATG 
    AGAAAACGGA GGGAAAAGAA ATTGGAGACG AAATTGAAGG AAATGATGTT TGGCGGAAGT 
    CTCAAAGTAT ACGGTGGAGA AATTGTGCCA ACTCGTCCAT ATGTCTCAAT ATTGGCTGAA 
    ATCAACGAAA ATGCTGATAG AATTTTGGGT GCTGCACTGG AAAAGTATGG ATTGGAACAT 
    TCGAAAGATG ATTTTATTCT TGTTGAGGTA TCCAACGACG ATGACCGCAA AAGTATGTCA 
    GACCTCCGTG AAATCGACGG ACGGCCCATT CCACCAACAG AGTGCCCGCT TTTCGAGATG 
    ACTGCTCGAA GTGGAAACGG CGAGAATGGA TTCGATTCGT TTTTGGCGAT CAAGCGGAAA 
    CCGCATGATT ATCAAGTCCC CTCCACCTCA GTTGTTGCTC CATCATCAGT CACCCTACCA 
    ATGTCTCCAT TAACATCACT TCCACCACAA ATTCACAATC TTCCAGTGCT TATTGTGATG 
    AATAATGATG GTCACTTGTC AACACACCGA ATAACGTTAC ACGAGGGAGT AACAGAGGTT 
    GGAAGTGACA GTCAAATGTC CAATTTCTCA CAGCATAATA TTTATCTCGA TGGTCCGGAT 
    ATTCGTGGAC GCCACGCGGC AATCGCTTTC ATGGAAGGCG TCGTCACACT AACACCATCC 
    ACTCGTGATG CCTACCTAGA GGTGAACGGC CATCAACTGA TGCAGACGGA GATCCTCCGG 
    GACGGTGATC ACATTCGCAT CGGAGCCGAT CATTCGTTCA AGTTTAGCAC GTCGTCGTCA 
    CAAGCAAATC AGATTAACAG TACTAATATC AGCAACTCGA ACCAACGACA CTTCCAACAA 
    CAGACATTTT ATCCGGAAAT GGCTGATTCA CGAGCTTCAA CTGCCAGGTG TGCACCATCG 
    GATTACTGTA GTCTACCACA ACAGAACGAT TATTATCCGC CGAGCATGTC CGGCTCATCA 
    GTCTCATCAT CACGGCAGGA GGATCTTATG ATGCATTCCA ATAATAACAT CCTGCCACCG 
    TACTCTACAA AATCCGCGCA GGAACCCCTC CAACTGCCGC TCTCAATTCA AATCGGCGAC 
    TACAATGTAT GGGATTTCCT GGCCGAAGTG ATGTCGAATC GTGCGGCAAC CTCATCGGAA 
    ATGTCCACAT TCCGCCTATC GCCTGCCTAC TCTCTCTACC TGGCACTTCG ATCATTCCAC 
    ACACACAATA AACCGTACTC TGTGCAATTC TTCTCACGGA TCGAGTCGAA TTTCAAGCAA 
    ATCGCTCAGG AATGCTCGTC GAAGGATGAT CTGATCTTTT GGCTTGCCAA CGCATCGGAA 
    TTCAATCATC TTGTCGAGCG GGACACTGAG CTGAAAGCGT CGAGATATGG CAAAATATCT 
    GTGGAAATTG AGAGAATATT CCGGCGATTG GTTGCAGTGC TTCAAAGTGT CATCCGTCCG 
    GCGGCGCGGG CTCTGTTGGA CATTCATCTG AATGATCACG ACGCGACCGG CGATGTGATT 
    CATCTTCTCG ACACCACGAT GCGCCAGGCG AGAATTTGTG GTCTCAACGC GGCGCTCACA 
    ATTCAACTGT GCGGGCATCT CCTCCACATG GTCAATGCCT TTGTGTTCAA CAGCTTGGTC 
    TCAGTCAACC TTCCAGCTGC CCAGTTGAAT ACTCGCCTCG GAAAGTGTCT TCAGTATCGG 
    ATAGAGTCGA TTCACAGATT CTGCGAACGA ATGGGCGTCG AGATGGCCGC CGAGTGCCAT 
    TTGGATAGAT CCCGTCAGGC CGCTAACCTT CTCGCCACGC AGAAGAATGA TGTTGCCGCG 
    TTGGGATCGA CGTGCTATAA GCTGAACAGC CTCCAAGTGG TTCATATCCT GTCGGGATTC 
    GAACCAGACG ACGGCGAGAT TCCATGTGAC GATGACGTCG TTGATCGGAT CTGTAGACTC 
    GCTGAAAAAC AGGCGGATAA GCTGACGTTG GAGGATGGGC AGCGCGTGAC ACTGGAGGAG 
    TCCGACGAGC TGAACCTTCC GTTCCTGATC CCTCAGGATG GTTACTTTGT CGAGCAGTTC 
    CGAACGGTGC CAGATGGTCT TTGGCAGTAC TTGCAGCAAC TTCAGCATAG GCGCCTCTGT 
    CATACGGTGT CGCTTACACC GGCACCGCCG GCTGCGATGA CCCATTCGAT GCATGCGATG 
    CATATTGGAG CAAGCGATGC TCATTATCAT CAGCATCAGC ATCAGCAGCA GCAGAACCCG 
    ATTGTGAGAA GTGTGTCGCA GACTACACTG ACCAGTATTC CGACTACACA ATTTGGCGAC 
    AGTTTTGGAA GTGCCGTGTC GGATACGATA TCGAAGGTGA CACTGCGGAA GAATGGAGGA 
    GGCATCGGAT TGAGTATTGT CGCTGCTCAG GGTGTTGGTG ATCGTCAAAT GGGAATTTAT 
    GTGAAGAAGG TTGTCGAAGG AACACCGGCT GCACACGATG GACGACTCGA GACTGGTGAT 
    CAGTTATTGA GTGTTAACGG ACATTCGTTA ATTGGAATTT CACAGGAAGA CGCCGCCCGT 
    CTCATGACCC AAAGTGGAAA CGAGGTGCAT TTCGACGTCC GGAAAAGAGC CGCTCACCGT 
    AACGGGCTCA GCAATTGGCT CGGATCCCAA ACACCGACAA CTTCAAATTT CATTGCACCA 
    TCTCCATCTA CAGTACCCCC GCCGAGTTAT TCACATGTTG GACGGACTCC GTCTTATGAT 
    GGACAACCAC CACCGAGTTA TAATCACAGA AATGGTGGAG CCAGCGGAGC AGGAGGATCA 
    TCTGGAAGAA ATAGTGCTTT CGTAGCACCA CTTTCGAATT CATCATCAAA TGCGTCGAGT 
    AGTCATATGC AACAACACTA TGCTCATCAT AATATGATGC ATAATGGCAA TTTGAGTGCT 
    GCTCCGGGAG CTCCCGCGGG GGATCCGAAA CATTATCGAA GCTCGTCGGC TAGTGATTTG 
    CAGCACGATC CAAACGCCTC ATTCTCTCAA TCATCAATGA CTGGAAGTGC GAGAAGTTCA 
    GTGTTTGACA AGCTTCCATC TCACTACCGT CACTCGACAC GGCCTACAGT AATCCAGCCA 
    ACACGTCCAA GTGCTCAATC TCCATCGGCT CTTCGAAAAG CGGCCGCCGC CTCGCCGGTC 
    AGCAATCTGT ACCGTCCGAC GTCGGCGTCG AATCTGTTTG CACCGCCCAG AAATTCGGGC 
    GGTCTCGTCG GATATTCGAG TAACGCGGCA CGGGAGTCGA CACAGGATAT TCATCGAGCG 
    GAGCCAGCCA TTGTTATCAG GCCTGTCGAC CTGAATATGC CGAAAGCGAC ACCACCGGCG 
    TCGATTCGTG GAGCTGTGCC GACGGCGATT ACACAGGCTC TTCAACTTCA ACCAAACGGC 
    GGTGGAGCAG TACTTCGTCA GGTTCGGCCA GCATCCGGGC TCCGCGAGCG ACTTCAGGAC 
    GCTGATGTTC GGAATTTGGA GAAAACGAAC ACAGCACTGA TGAGCTATGA AGCAGTGAAT 
    GAGGAGCTCG ATCGACTCGA CGCTAAGCAG AATACGATGA CAATTGACGA GCAACGGAGA 
    TATCGAGAGC TACTGAACGT TGTCGCCGAG CAGAGCCGAG CAAGGAAGCT GCTCTCGGAG 
    ATCAGATCAC CACCTACATC TTATCAAGAA CGTGAAATTC CGATTCATGT GCAAAAAGAG 
    CAGCAGCAGC ACCGGCGGGA AACTGTGATT GATGATATTC CACACTCGAG CTCAAGTGTT 
    GAGAATGTGT CACCTGTGAG CCCGCCGGAA AGTCGAAAAG TTCAATTTAT TGATGAAGTT 
    GTGCAGTATC AGACGCCGAC CAGCGGAGCC AATCACGCTT TCTTTGATGA TGTGCCGGTT 
    TTGGATTCGC CTGGGATCGT CGGCACCAAC GAAATCTACC GTGATCCCCG CCAACGTCGG 
    CTCAACGAGC TCCAAGACCG CAATCGCAGC TCGGAAAATG GAGCGGCGGA CGGCGCGAAG 
    CTCGGCTTCC GCGACAAGCA GCGCCTATTC GCACGGCAAA TCGGCGAGGA CACGGCACCA 
    CGCCAACGAA TGAATGAGAG CAGTGCTCAG CGGCTCATTG AGACGGACAT TGCCGCCACC 
    CCGCAAAAGC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    afd-1 ( NM_058537.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_490938.3 Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.
    Price & Availability↑
    CloneID OCa336875
    Clone ID Related Accession (Same CDS sequence) NM_058537.4 , NM_058537.5
    Accession Version NM_058537.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product PDZ domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_058537.4. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTAC AAGTAAACGG AGGACGAGAA GAAGCGCAAT CTCAAGATGA TATCTATAAA 
    CAAGTACCAT TTAATACATT CACCCGAACT ATATCTAATC CAGAAGCTGT TATGAGAAAA 
    CGGAGGGAAA AGAAATTGGA GACGAAATTG AAGGAAATGA TGTTTGGCGG AAGTCTCAAA 
    GTATACGGTG GAGAAATTGT GCCAACTCGT CCATATGTCT CAATATTGGC TGAAATCAAC 
    GAAAATGCTG ATAGAATTTT GGGTGCTGCA CTGGAAAAGT ATGGATTGGA ACATTCGAAA 
    GATGATTTTA TTCTTGTTGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_490938.3
    CDS30..5006
    Translation

    Target ORF information:

    RefSeq Version NM_058537.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans PDZ domain-containing protein (afd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_058537.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTATCAG AACGTGAACA GCTCGCCTGT CTTGTACAAC AGTGGAATGA GAATCGTCTT 
    GATTTATTTC ATCTCTCCTA TCCAACAGAA GATCTTGAGG TCGAAGGTGT CATGCGGTTC 
    TATTTTCAGG ACGGCGGTGA AAAAGTGCTA ACAAAATGCG TCCGTGTCTC GTCAACAGCT 
    ACAACACGTG CAGTAGTTGA TGCACTTTCA GAGAAATTTC TGCCAGATCT TAAAATGTTA 
    TCAAATGATA CATATTCACT GTGGGAAGTA CATGAAAATG GAGAAGAACG GAAATTATTG 
    GATGAGGAAA AGCCATTAGT TGTACAATTA CAATGGCATA AAGATGATCG AGAAGGACGC 
    TTTTTGCTGA AAAAAGATGC GGCGCCACCG GTTTCTCTTT TGAATCTCCA AGAAGCCGAT 
    CAGAAAAATA CCCGATTCGC AAAAAAGAAG GATAAAGAGC CGAAAAAGAA AAATCAAAAA 
    AATTTTGTAC AAGTAAACGG AGGACGAGAA GAAGCGCAAT CTCAAGATGA TATCTATAAA 
    CAAGTACCAT TTAATACATT CACCCGAACT ATATCTAATC CAGAAGCTGT TATGAGAAAA 
    CGGAGGGAAA AGAAATTGGA GACGAAATTG AAGGAAATGA TGTTTGGCGG AAGTCTCAAA 
    GTATACGGTG GAGAAATTGT GCCAACTCGT CCATATGTCT CAATATTGGC TGAAATCAAC 
    GAAAATGCTG ATAGAATTTT GGGTGCTGCA CTGGAAAAGT ATGGATTGGA ACATTCGAAA 
    GATGATTTTA TTCTTGTTGA GGTATCCAAC GACGATGACC GCAAAAGTAT GTCAGACCTC 
    CGTGAAATCG ACGGACGGCC CATTCCACCA ACAGAGTGCC CGCTTTTCGA GATGACTGCT 
    CGAAGTGGAA ACGGCGAGAA TGGATTCGAT TCGTTTTTGG CGATCAAGCG GAAACCGCAT 
    GATTATCAAG TCCCCTCCAC CTCAGTTGTT GCTCCATCAT CAGTCACCCT ACCAATGTCT 
    CCATTAACAT CACTTCCACC ACAAATTCAC AATCTTCCAG TGCTTATTGT GATGAATAAT 
    GATGGTCACT TGTCAACACA CCGAATAACG TTACACGAGG GAGTAACAGA GGTTGGAAGT 
    GACAGTCAAA TGTCCAATTT CTCACAGCAT AATATTTATC TCGATGGTCC GGATATTCGT 
    GGACGCCACG CGGCAATCGC TTTCATGGAA GGCGTCGTCA CACTAACACC ATCCACTCGT 
    GATGCCTACC TAGAGGTGAA CGGCCATCAA CTGATGCAGA CGGAGATCCT CCGGGACGGT 
    GATCACATTC GCATCGGAGC CGATCATTCG TTCAAGTTTA GCACGTCGTC GTCACAAGCA 
    AATCAGATTA ACAGTACTAA TATCAGCAAC TCGAACCAAC GACACTTCCA ACAACAGACA 
    TTTTATCCGG AAATGGCTGA TTCACGAGCT TCAACTGCCA GGTGTGCACC ATCGGATTAC 
    TGTAGTCTAC CACAACAGAA CGATTATTAT CCGCCGAGCA TGTCCGGCTC ATCAGTCTCA 
    TCATCACGGC AGGAGGATCT TATGATGCAT TCCAATAATA ACATCCTGCC ACCGTACTCT 
    ACAAAATCCG CGCAGGAACC CCTCCAACTG CCGCTCTCAA TTCAAATCGG CGACTACAAT 
    GTATGGGATT TCCTGGCCGA AGTGATGTCG AATCGTGCGG CAACCTCATC GGAAATGTCC 
    ACATTCCGCC TATCGCCTGC CTACTCTCTC TACCTGGCAC TTCGATCATT CCACACACAC 
    AATAAACCGT ACTCTGTGCA ATTCTTCTCA CGGATCGAGT CGAATTTCAA GCAAATCGCT 
    CAGGAATGCT CGTCGAAGGA TGATCTGATC TTTTGGCTTG CCAACGCATC GGAATTCAAT 
    CATCTTGTCG AGCGGGACAC TGAGCTGAAA GCGTCGAGAT ATGGCAAAAT ATCTGTGGAA 
    ATTGAGAGAA TATTCCGGCG ATTGGTTGCA GTGCTTCAAA GTGTCATCCG TCCGGCGGCG 
    CGGGCTCTGT TGGACATTCA TCTGAATGAT CACGACGCGA CCGGCGATGT GATTCATCTT 
    CTCGACACCA CGATGCGCCA GGCGAGAATT TGTGGTCTCA ACGCGGCGCT CACAATTCAA 
    CTGTGCGGGC ATCTCCTCCA CATGGTCAAT GCCTTTGTGT TCAACAGCTT GGTCTCAGTC 
    AACCTTCCAG CTGCCCAGTT GAATACTCGC CTCGGAAAGT GTCTTCAGTA TCGGATAGAG 
    TCGATTCACA GATTCTGCGA ACGAATGGGC GTCGAGATGG CCGCCGAGTG CCATTTGGAT 
    AGATCCCGTC AGGCCGCTAA CCTTCTCGCC ACGCAGAAGA ATGATGTTGC CGCGTTGGGA 
    TCGACGTGCT ATAAGCTGAA CAGCCTCCAA GTGGTTCATA TCCTGTCGGG ATTCGAACCA 
    GACGACGGCG AGATTCCATG TGACGATGAC GTCGTTGATC GGATCTGTAG ACTCGCTGAA 
    AAACAGGCGG ATAAGCTGAC GTTGGAGGAT GGGCAGCGCG TGACACTGGA GGAGTCCGAC 
    GAGCTGAACC TTCCGTTCCT GATCCCTCAG GATGGTTACT TTGTCGAGCA GTTCCGAACG 
    GTGCCAGATG GTCTTTGGCA GTACTTGCAG CAACTTCAGC ATAGGCGCCT CTGTCATACG 
    GTGTCGCTTA CACCGGCACC GCCGGCTGCG ATGACCCATT CGATGCATGC GATGCATATT 
    GGAGCAAGCG ATGCTCATTA TCATCAGCAT CAGCATCAGC AGCAGCAGAA CCCGATTGTG 
    AGAAGTGTGT CGCAGACTAC ACTGACCAGT ATTCCGACTA CACAATTTGG CGACAGTTTT 
    GGAAGTGCCG TGTCGGATAC GATATCGAAG GTGACACTGC GGAAGAATGG AGGAGGCATC 
    GGATTGAGTA TTGTCGCTGC TCAGGGTGTT GGTGATCGTC AAATGGGAAT TTATGTGAAG 
    AAGGTTGTCG AAGGAACACC GGCTGCACAC GATGGACGAC TCGAGACTGG TGATCAGTTA 
    TTGAGTGTTA ACGGACATTC GTTAATTGGA ATTTCACAGG AAGACGCCGC CCGTCTCATG 
    ACCCAAAGTG GAAACGAGGT GCATTTCGAC GTCCGGAAAA GAGCCGCTCA CCGTAACGGG 
    CTCAGCAATT GGCTCGGATC CCAAACACCG ACAACTTCAA ATTTCATTGC ACCATCTCCA 
    TCTACAGTAC CCCCGCCGAG TTATTCACAT GTTGGACGGA CTCCGTCTTA TGATGGACAA 
    CCACCACCGA GTTATAATCA CAGAAATGGT GGAGCCAGCG GAGCAGGAGG ATCATCTGGA 
    AGAAATAGTG CTTTCGTAGC ACCACTTTCG AATTCATCAT CAAATGCGTC GAGTAGTCAT 
    ATGCAACAAC ACTATGCTCA TCATAATATG ATGCATAATG GCAATTTGAG TGCTGCTCCG 
    GGAGCTCCCG CGGGGGATCC GAAACATTAT CGAAGCTCGT CGGCTAGTGA TTTGCAGCAC 
    GATCCAAACG CCTCATTCTC TCAATCATCA ATGACTGGAA GTGCGAGAAG TTCAGTGTTT 
    GACAAGCTTC CATCTCACTA CCGTCACTCG ACACGGCCTA CAGTAATCCA GCCAACACGT 
    CCAAGTGCTC AATCTCCATC GGCTCTTCGA AAAGCGGCCG CCGCCTCGCC GGTCAGCAAT 
    CTGTACCGTC CGACGTCGGC GTCGAATCTG TTTGCACCGC CCAGAAATTC GGGCGGTCTC 
    GTCGGATATT CGAGTAACGC GGCACGGGAG TCGACACAGG ATATTCATCG AGCGCTAAAT 
    GACCTGGAAC TCAACTCACC GCTCGCCAGA TCCACGCCGA ATGAGGAGTA CCGTATCCCG 
    CCGCCTAGAG TACCTACAGT AACCTCCACC TCCTCCAACT ATGCCCCTCT TCATTCTATT 
    CGACCGGCAA GCATGTATGG ATATCAGAGC CCGAGCTCTT CTACTACAGT AATCCCGGCT 
    CCAAAAGGAG TGGCTTCTTC CTCTAATAAC ACTTCTCCCC GAGTGAATGT GACGTTGGGT 
    GGAGCATTGA GCTCATCGAG ATCTCATAAT AGTCTTTCAC AGGAGCCAGC CATTGTTATC 
    AGGCCTGTCG ACCTGAATAT GCCGAAAGCG ACACCACCGG CGTCGATTCG TGGAGCTGTG 
    CCGACGGCGA TTACACAGGC TCTTCAACTT CAACCAAACG GCGGTGGAGC AGTACTTCGT 
    CAGGTTCGGC CAGCATCCGG GCTCCGCGAG CGACTTCAGG ACGCTGATGT TCGGAATTTG 
    GAGAAAACGA ACACAGCACT GATGAGCTAT GAAGCAGTGA ATGAGGAGCT CGATCGACTC 
    GACGCTAAGC AGAATACGAT GACAATTGAC GAGCAACGGA GATATCGAGA GCTACTGAAC 
    GTTGTCGCCG AGCAGAGCCG AGCAAGGAAG CTGCTCTCGG AGATCAGATC ACCACCTACA 
    TCTTATCAAG AACGTGAAAT TCCGATTCAT GTGCAAAAAG AGCAGCAGCA GCACCGGCGG 
    GAAACTGTGA TTGATGATAT TCCACACTCG AGCTCAAGTG TTGAGAATGT GTCACCTGTG 
    AGCCCGCCGG AAAGTCGAAA AGTTCAATTT ATTGATGAAG TTGTGCAGTA TCAGACGCCG 
    ACCAGCGGAG CCAATCACGC TTTCTTTGAT GATGTGCCGG TTTTGGATTC GCCTGGGATC 
    GTCGGCACCA ACGAAATCTA CCGTGATCCC CGCCAACGTC GGCTCAACGA GCTCCAAGAC 
    CGCAATCGCA GCTCGGAAAA TGGAGCGGCG GACGGCGCGA AGCTCGGCTT CCGCGACAAG 
    CAGCGCCTAT TCGCACGGCA AATCGGCGAG GACACGGCAC CACGCCAACG AATGAATGAG 
    AGCAGTGCTC AGCGGCTCAT TGAGACGGAC ATTGCCGCCA CCCCGCAAAA GCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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