AFDN gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005266996.3	XP_005267053.1	afadin isoform X6
XM_017010885.1	XP_016866374.1	afadin isoform X11
XM_006715491.2	XP_006715554.1	afadin isoform X13
XM_017010887.1	XP_016866376.1	afadin isoform X14
NM_001366319.1	NP_001353248.1	afadin isoform 5
NM_001040000.2	NP_001035089.1	afadin isoform 2
NM_001366321.2	NP_001353250.1	afadin isoform 7
NM_001207008.1	NP_001193937.1	afadin isoform 1
XM_017010883.1	XP_016866372.1	afadin isoform X8
XM_017010878.1	XP_016866367.1	afadin isoform X1
XM_017010881.1	XP_016866370.1	afadin isoform X4
XM_005266997.2	XP_005267054.1	afadin isoform X7
XM_017010884.1	XP_016866373.1	afadin isoform X10
XM_017010886.1	XP_016866375.1	afadin isoform X12
XM_006715494.3	XP_006715557.1	afadin isoform X15
NM_001291964.1	NP_001278893.1	afadin isoform 4
NM_001040000.3	NP_001035089.1	afadin isoform 2
NM_001366320.1	NP_001353249.1	afadin isoform 6
XM_011535842.2	XP_011534144.1	afadin isoform X13
XM_017010880.1	XP_016866369.1	afadin isoform X3
NM_001366321.1	NP_001353250.1	afadin isoform 7
XM_017010879.1	XP_016866368.1	afadin isoform X2
XM_017010882.1	XP_016866371.1	afadin isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24568	NM_001291964.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu26785	NM_001040000.3 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu107064	NM_001366319.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu96620	NM_001366321.2 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu96623	XM_017010886.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu24535	NM_001207008.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu40829	XM_006715491.2 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu40832	XM_006715494.3 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu59006	XM_011535842.2 Latest version!	Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu40825	NM_001366320.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu40825	XM_005266996.3 Latest version!	Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu40826	XM_005266997.2 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu96615	XM_017010878.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu96616	XM_017010879.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu96617	XM_017010880.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu96618	XM_017010881.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu96619	XM_017010882.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu96620	XM_017010883.1 Latest version!	Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu96621	XM_017010884.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu96622	XM_017010885.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu96624	XM_017010887.1 Latest version!	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OHu26785	NM_001040000.2	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu96620	NM_001366321.1	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

AFDN ( NM_001291964.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278893.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu24568
Clone ID Related Accession (Same CDS sequence)	NM_001291964.1
Accession Version	NM_001291964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	afadin isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB621809.1, AL009178.4, AL049698.3 and AL161973.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB621809.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTAATC GAAACGCTCG CGGAGAAATT TCGACCTGAT 
ATGCGAATGC TGTCCTCTCC CAAGTATTCA CTCTATGAAG TGCATGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCTCTA GTTGTACAAC TGAATTGGAA CAAAGATGAT 
CGGGAAGGCA GATTTGTTCT TAAGAATGAG AATGACGCCA TTCCTCCTAA GAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT 
CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT 
GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG 
TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG 
CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGCTT CTGGGCCTGA GCTGATACTA 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT 
AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGTGTCAT CCAGAAACAG 
AAGAATATTG CAGGGGCACT TGCCTTCTGG ATGGCAAATG CATCTGAACT TCTCAACTTC 
ATTAAGCAAG ACCGAGACCT TAGTCGGATC ACACTGGATG CTCAAGATGT TTTAGCACAT 
TTGGTTCAAA TGGCATTTAA ATACTTGGTT CACTGTCTTC AATCAGAACT TAATAATTAC 
ATGCCAGCCT TTCTAGATGA CCCTGAAGAG AACAGTCTGC AACGACCAAA AATAGATGAT 
GTGCTGCACA CGCTCACAGG AGCCATGTCC TTGCTACGAC GCTGCAGAGT CAATGCCGCC 
CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTCATCA ATATGTGGCT GTTCAATAGA 
TTGGTGACCG ACCCAGATTC GGGGCTGTGC TCCCATTACT GGGGTGCGAT TATCCGTCAG 
CAGTTGGGCC ATATTGAAGC CTGGGCTGAG AAGCAGGGGC TGGAACTGGC TGCGGACTGT 
CATCTGAGCA GGATCGTGCA GGCAACGACT TTGCTTACCA TGGATAAGTA TGCACCTGAT 
GACATTCCAA ATATAAACAG CACCTGCTTT AAGTTAAATT CATTACAACT TCAAGCCTTA 
TTACAGAACT ATCACTGTGC ACCTGATGAG CCTTTTATCC CAACGGATCT TATAGAAAAT 
GTAGTGACTG TGGCTGAAAA CACTGCCGAT GAGCTGGCCC GCAGTGATGG AAGGGAAGTG 
CAGTTGGAGG AGGATCCTGA TCTGCAGCTG CCGTTTCTTT TGCCAGAAGA TGGTTATTCT 
TGTGATGTTG TCAGAAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TCTGTGCCAG 
AGAGGATTTT GCAGGTTAAT TCCTCACACA CGTTCACCAG GTACTTGGAC AATATATTTT 
GAAGGTGCAG ATTATGAAAG TCACCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CTGAAATAAT CACTGTGACC CTAAAAAAGC AGAATGGAAT GGGCCTTAGC 
ATTGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTAGGAA TCTATGTGAA GTCGGTTGTG 
AAAGGAGGTG CTGCAGATGT GGATGGACGT CTAGCTGCAG GTGATCAGCT CCTCAGTGTG 
GATGGACGAA GTCTGGTTGG ACTCTCTCAG GAAAGGGCGG CAGAACTCAT GACAAGAACA 
AGCTCTGTGG TGACACTGGA AGTAGCAAAG CAGGGTGCCA TCTACCACGG TCTGGCCACC 
CTTCTCAATC AGCCATCCCC CATGATGCAG AGAATTTCAG ATCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGTGAAGG CTTTGAGCTC TATAATAATT CAACTCAAAA TGGGTCTCCT 
GAGAGTCCTC AGCTGCCTTG GGCAGAATAT AGTGAACCAA AGAAATTGCC TGGTGATGAC 
AGACTGATGA AAAATAGAGC TGATCACCGT TCCAGCCCCA ACGTAGCAAA TCAGCCTCCT 
AGTCCTGGAG GGAAAAGTGC ATATGCCTCT GGAACAACAG CGAAGATAAC ATCTGTCTCT 
ACTGGAAACC TCTGCACTGA GGAGCAGACG CCTCCGCCTA GACCTGAAGC CTACCCCATC 
CCCACTCAGA CGTACACCAG AGAGTATTTT ACCTTCCCAG CTTCCAAATC CCAGGATCGG 
ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TATGAGGAAA AGCCACATAT GCACACAGAT 
AGTAATCATT CCAGTATTGC AATTCAGCGT GTTACACGTT CCCAAGAAGA ACTTCGAGAA 
GATAAAGCTT ACCAACTTGA GCGGCATCGA ATAGAGGCAG CTATGGACCG AAAGTCTGAT 
AGTGATATGT GGATAAATCA GAGCTCCTCA CTGGACTCCA GTACCTCTAG CCAGGAGCAT 
CTGAACCATT CCTCTAAGTC GGTCACCCCT GCTTCCACAC TGACCAAAAG TGGCCCTGGC 
CGTTGGAAAA CACCAGCAGC CATACCGGCC ACCCCTGTGG CCGTCTCCCA GCCAATCCGA 
ACAGACCTGC CTCCGCCACC CCCGCCACCT CCAGTCCACT ATGCCGGTGA TTTCGATGGA 
ATGTCCATGG ATTTGCCTCT CCCACCACCC CCTTCCGCCA ACCAGATAGG GCTGCCGTCT 
GCGCAGGTGG CTGCTGCTGA ACGGAGAAAG AGAGAAGAAC ATCAGCGTTG GTATGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAG AGGAAGCGGA GAGAGCAGGA GAGGAAGTTG 
GGCCAGATGC GCACTCAGTC CTTAAACCCT GCTCCGTTTT CTCCCCTGAC TGCACAGCAG 
ATGAAGCCCG AAAAGCCTTC CACACTCCAG CGGCCACAGG AAACAGTCAT TCGGGAGCTG 
CAGCCTCAGC AGCAGCCCCG CACGATCGAG CGCAGAGACT TGCAGTACAT TACAGTCAGC 
AAAGAGGAGC TTTCCTCGGG GGACAGTCTG TCCCCCGACC CGTGGAAGCG GGACGCCAAG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC ATCGTGGACA TGCTGAGCAA GGAGATCCAG 
GAGCTCCAGA GCAAACCGGA CCGCAGCGCC GAGGAGAGCG ACCGGCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTCCAG GAGTCGAAGC AGAAGGACGA AGATGACGAG 
GAGGAGGAGG ACGATGATGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCTGAACGA 
AGAGCGAGGG ACGAGGAGCG GAGGCGGCAG CAGCAGTTAG AAGAGATGCG CAAGCGGGAA 
GCGGAAGACC GAGCGAGGCA AGAGGAAGAG CGCCGGCGGC AGGAGGAGGA GCGAACAAAA 
CGAGACGCTG AAGAAAAGAG GCGACAGGAA GAAGGGTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGACGA GGCGGCGCGC AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG 
TGCCGCCCTC CGCTTCCCCG GGACTACGAG CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC 
CCTCCTCCCC CGCCTCAGCG AAACGCCTCC TACCTCAAAA CACAGGTCCT CTCCCCCGAC 
TCGCTGTTCA CTGCCAAGTT TGTTGCATAC AATGAGGAGG AGGAGGAGGA GGACTGCAGC 
CTAGCAGGAC CAAACTCTTA CCCAGGATCT ACTGGAGCAG CTGTTGGAGC CCATGACGCC 
TGTCGGGATG CAAAAGAGAA GCGCTCTAAA AGCCAAGATG CAGATTCACC TGGAAGTTCT 
GGGGCCCCTG AAAACTTGAC ATTCAAGGAA CGCCAGCGTC TTTTTTCACA AGGTCAAGAT 
GTATCCAATA AAGTGAAAGC TTCTCGTAAA TTAACAGAAC TGGAGAATGA ACTGAACACA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278893.1
CDS	320..5665
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	NM_001291964.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291964.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTAATC GAAACGCTCG CGGAGAAATT TCGACCTGAT 
ATGCGAATGC TGTCCTCTCC CAAGTATTCA CTCTATGAAG TGCATGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCTCTA GTTGTACAAC TGAATTGGAA CAAAGATGAT 
CGGGAAGGCA GATTTGTTCT TAAGAATGAG AATGACGCCA TTCCTCCTAA GAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT 
CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT 
GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG 
TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG 
CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGCTT CTGGGCCTGA GCTGATACTA 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT 
AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGTGTCAT CCAGAAACAG 
AAGAATATTG CAGGGGCACT TGCCTTCTGG ATGGCAAATG CATCTGAACT TCTCAACTTC 
ATTAAGCAAG ACCGAGACCT TAGTCGGATC ACACTGGATG CTCAAGATGT TTTAGCACAT 
TTGGTTCAAA TGGCATTTAA ATACTTGGTT CACTGTCTTC AATCAGAACT TAATAATTAC 
ATGCCAGCCT TTCTAGATGA CCCTGAAGAG AACAGTCTGC AACGACCAAA AATAGATGAT 
GTGCTGCACA CGCTCACAGG AGCCATGTCC TTGCTACGAC GCTGCAGAGT CAATGCCGCC 
CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTCATCA ATATGTGGCT GTTCAATAGA 
TTGGTGACCG ACCCAGATTC GGGGCTGTGC TCCCATTACT GGGGTGCGAT TATCCGTCAG 
CAGTTGGGCC ATATTGAAGC CTGGGCTGAG AAGCAGGGGC TGGAACTGGC TGCGGACTGT 
CATCTGAGCA GGATCGTGCA GGCAACGACT TTGCTTACCA TGGATAAGTA TGCACCTGAT 
GACATTCCAA ATATAAACAG CACCTGCTTT AAGTTAAATT CATTACAACT TCAAGCCTTA 
TTACAGAACT ATCACTGTGC ACCTGATGAG CCTTTTATCC CAACGGATCT TATAGAAAAT 
GTAGTGACTG TGGCTGAAAA CACTGCCGAT GAGCTGGCCC GCAGTGATGG AAGGGAAGTG 
CAGTTGGAGG AGGATCCTGA TCTGCAGCTG CCGTTTCTTT TGCCAGAAGA TGGTTATTCT 
TGTGATGTTG TCAGAAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TCTGTGCCAG 
AGAGGATTTT GCAGGTTAAT TCCTCACACA CGTTCACCAG GTACTTGGAC AATATATTTT 
GAAGGTGCAG ATTATGAAAG TCACCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CTGAAATAAT CACTGTGACC CTAAAAAAGC AGAATGGAAT GGGCCTTAGC 
ATTGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTAGGAA TCTATGTGAA GTCGGTTGTG 
AAAGGAGGTG CTGCAGATGT GGATGGACGT CTAGCTGCAG GTGATCAGCT CCTCAGTGTG 
GATGGACGAA GTCTGGTTGG ACTCTCTCAG GAAAGGGCGG CAGAACTCAT GACAAGAACA 
AGCTCTGTGG TGACACTGGA AGTAGCAAAG CAGGGTGCCA TCTACCACGG TCTGGCCACC 
CTTCTCAATC AGCCATCCCC CATGATGCAG AGAATTTCAG ATCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGTGAAGG CTTTGAGCTC TATAATAATT CAACTCAAAA TGGGTCTCCT 
GAGAGTCCTC AGCTGCCTTG GGCAGAATAT AGTGAACCAA AGAAATTGCC TGGTGATGAC 
AGACTGATGA AAAATAGAGC TGATCACCGT TCCAGCCCCA ACGTAGCAAA TCAGCCTCCT 
AGTCCTGGAG GGAAAAGTGC ATATGCCTCT GGAACAACAG CGAAGATAAC ATCTGTCTCT 
ACTGGAAACC TCTGCACTGA GGAGCAGACG CCTCCGCCTA GACCTGAAGC CTACCCCATC 
CCCACTCAGA CGTACACCAG AGAGTATTTT ACCTTCCCAG CTTCCAAATC CCAGGATCGG 
ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TATGAGGAAA AGCCACATAT GCACACAGAT 
AGTAATCATT CCAGTATTGC AATTCAGCGT GTTACACGTT CCCAAGAAGA ACTTCGAGAA 
GATAAAGCTT ACCAACTTGA GCGGCATCGA ATAGAGGCAG CTATGGACCG AAAGTCTGAT 
AGTGATATGT GGATAAATCA GAGCTCCTCA CTGGACTCCA GTACCTCTAG CCAGGAGCAT 
CTGAACCATT CCTCTAAGTC GGTCACCCCT GCTTCCACAC TGACCAAAAG TGGCCCTGGC 
CGTTGGAAAA CACCAGCAGC CATACCGGCC ACCCCTGTGG CCGTCTCCCA GCCAATCCGA 
ACAGACCTGC CTCCGCCACC CCCGCCACCT CCAGTCCACT ATGCCGGTGA TTTCGATGGA 
ATGTCCATGG ATTTGCCTCT CCCACCACCC CCTTCCGCCA ACCAGATAGG GCTGCCGTCT 
GCGCAGGTGG CTGCTGCTGA ACGGAGAAAG AGAGAAGAAC ATCAGCGTTG GTATGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAG AGGAAGCGGA GAGAGCAGGA GAGGAAGTTG 
GGCCAGATGC GCACTCAGTC CTTAAACCCT GCTCCGTTTT CTCCCCTGAC TGCACAGCAG 
ATGAAGCCCG AAAAGCCTTC CACACTCCAG CGGCCACAGG AAACAGTCAT TCGGGAGCTG 
CAGCCTCAGC AGCAGCCCCG CACGATCGAG CGCAGAGACT TGCAGTACAT TACAGTCAGC 
AAAGAGGAGC TTTCCTCGGG GGACAGTCTG TCCCCCGACC CGTGGAAGCG GGACGCCAAG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC ATCGTGGACA TGCTGAGCAA GGAGATCCAG 
GAGCTCCAGA GCAAACCGGA CCGCAGCGCC GAGGAGAGCG ACCGGCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTCCAG GAGTCGAAGC AGAAGGACGA AGATGACGAG 
GAGGAGGAGG ACGATGATGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCTGAACGA 
AGAGCGAGGG ACGAGGAGCG GAGGCGGCAG CAGCAGTTAG AAGAGATGCG CAAGCGGGAA 
GCGGAAGACC GAGCGAGGCA AGAGGAAGAG CGCCGGCGGC AGGAGGAGGA GCGAACAAAA 
CGAGACGCTG AAGAAAAGAG GCGACAGGAA GAAGGGTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGACGA GGCGGCGCGC AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG 
TGCCGCCCTC CGCTTCCCCG GGACTACGAG CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC 
CCTCCTCCCC CGCCTCAGCG AAACGCCTCC TACCTCAAAA CACAGGTCCT CTCCCCCGAC 
TCGCTGTTCA CTGCCAAGTT TGTTGCATAC AATGAGGAGG AGGAGGAGGA GGACTGCAGC 
CTAGCAGGAC CAAACTCTTA CCCAGGATCT ACTGGAGCAG CTGTTGGAGC CCATGACGCC 
TGTCGGGATG CAAAAGAGAA GCGCTCTAAA AGCCAAGATG CAGATTCACC TGGAAGTTCT 
GGGGCCCCTG AAAACTTGAC ATTCAAGGAA CGCCAGCGTC TTTTTTCACA AGGTCAAGAT 
GTATCCAATA AAGTGAAAGC TTCTCGTAAA TTAACAGAAC TGGAGAATGA ACTGAACACA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( NM_001040000.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035089.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 2, mRNA.

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CloneID	OHu26785
Clone ID Related Accession (Same CDS sequence)	NM_001040000.2 , NM_001040000.3
Accession Version	NM_001040000.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	afadin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI271860.1, AB209420.1, AK055689.1, D60168.1 and BE503462.1. On Jun 12, 2010 this sequence version replaced NM_001040000.1. Transcript Variant: This variant (2) has several differences compared to variant 4. The resulting isoform (2) is longer at the N-terminus but has a shorter and distinct C-terminus compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GGTTATGGTC CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035089.1
CDS	143..5098
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKVMVL

Target ORF information:

RefSeq Version	NM_001040000.2
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040000.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GGTTATGGTC CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( NM_001040000.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035089.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26785
Clone ID Related Accession (Same CDS sequence)	NM_001040000.2 , NM_001040000.3
Accession Version	NM_001040000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	afadin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL009178.4, AI271860.1, AB209420.1, AK055689.1, D60168.1 and BE503462.1. On Jun 2, 2019 this sequence version replaced NM_001040000.2. Transcript Variant: This variant (2) has several differences compared to variant 4. The resulting isoform (2) is longer at the N-terminus but has a shorter and distinct C-terminus compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GGTTATGGTC CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035089.1
CDS	223..5178
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKVMVL

Target ORF information:

RefSeq Version	NM_001040000.3
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040000.3

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GGTTATGGTC CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( NM_001366319.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353248.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu107064
Clone ID Related Accession (Same CDS sequence)	NM_001366319.1
Accession Version	NM_001366319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	afadin isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL009178.4, KT258903.1 and AL049698.3. On Sep 29, 2018 this sequence version replaced XM_024446446.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KT258903.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAGCCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GACCAAACTC TTACCCAGGA TCTACTGGAG CAGCTGTTGG AGCCCATGAC 
GCCTGTCGGG ATGCAAAAGA GAAGCGCTCT AAAAGCCAAG ATGCAGATTC ACCTGGAAGT 
TCTGGGGCCC CTGAAAACTT GACATTCAAG GAACGCCAGC GTCTTTTTTC ACAAGGTCAA 
GATGTATCCA ATAAAGTGAA AGCTTCTCGT AAATTAACAG AACTGGAGAA TGAACTGAAC 
ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353248.1
CDS	570..6038
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	NM_001366319.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366319.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAGCCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GACCAAACTC TTACCCAGGA TCTACTGGAG CAGCTGTTGG AGCCCATGAC 
GCCTGTCGGG ATGCAAAAGA GAAGCGCTCT AAAAGCCAAG ATGCAGATTC ACCTGGAAGT 
TCTGGGGCCC CTGAAAACTT GACATTCAAG GAACGCCAGC GTCTTTTTTC ACAAGGTCAA 
GATGTATCCA ATAAAGTGAA AGCTTCTCGT AAATTAACAG AACTGGAGAA TGAACTGAAC 
ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( NM_001366321.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353250.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 7, mRNA.

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CloneID	OHu96620
Clone ID Related Accession (Same CDS sequence)	NM_001366321.2 , XM_017010883.1 , NM_001366321.1
Accession Version	NM_001366321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	afadin isoform 7
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AL009178.4, AL731868.5 and AL049698.3. On Nov 30, 2019 this sequence version replaced NM_001366321.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT 
TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT 
GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353250.1
CDS	223..5676
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	NM_001366321.2
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366321.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT 
TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT 
GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017010886.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866375.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

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CloneID	OHu96623
Clone ID Related Accession (Same CDS sequence)	XM_017010886.1
Accession Version	XM_017010886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG 
CCCGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGGGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866375.1
CDS	257..5599
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017010886.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010886.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG 
CCCGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGGGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( NM_001207008.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001193937.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 1, mRNA.

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CloneID	OHu24535
Clone ID Related Accession (Same CDS sequence)	NM_001207008.1
Accession Version	NM_001207008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	afadin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE645141.1, AB209420.1, DA200453.1, DB079271.1, AL161973.1 and AL049698.3. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) has multiple differences compared to variant 4. The resulting isoform (1, also known as AF6i3) contains a C-terminal F-actin-binding site and has been shown to be involved in the regulation of epithelial wound closure and intercellular adhesion (PMID:16882694). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG 
CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGATGGAC GTCTAGCTGC AGGTGATCAG 
CTCCTCAGTG TGGATGGACG AAGTCTGGTT GGACTCTCTC AGGAAAGGGC GGCAGAACTC 
ATGACAAGAA CAAGCTCTGT GGTGACACTG GAAGTAGCAA AGCAGGGTGC CATCTACCAC 
GGTCTGGCCA CCCTTCTCAA TCAGCCATCC CCCATGATGC AGAGAATTTC AGATCGTCGT 
GGCTCAGGTA AACCCCGACC AAAGAGTGAA GGCTTTGAGC TCTATAATAA TTCAACTCAA 
AATGGGTCTC CTGAGAGTCC TCAGCTGCCT TGGGCAGAAT ATAGTGAACC AAAGAAATTG 
CCTGGTGATG ACAGACTGAT GAAAAATAGA GCTGATCACC GTTCCAGCCC CAACGTAGCA 
AATCAGCCTC CTAGTCCTGG AGGGAAAAGT GCATATGCCT CTGGAACAAC AGCGAAGATA 
ACATCTGTCT CTACTGGAAA CCTCTGCACT GAGGAGCAGA CGCCTCCGCC TAGACCTGAA 
GCCTACCCCA TCCCCACTCA GACGTACACC AGAGAGTATT TTACCTTCCC AGCTTCCAAA 
TCCCAGGATC GGATGGCTCC TCCTCAGAAC CAGTGGCCAA ATTATGAGGA AAAGCCACAT 
ATGCACACAG ATAGTAATCA TTCCAGTATT GCAATTCAGC GTGTTACACG TTCCCAAGAA 
GAACTTCGAG AAGATAAAGC TTACCAACTT GAGCGGCATC GAATAGAGGC AGCTATGGAC 
CGAAAGTCTG ATAGTGATAT GTGGATAAAT CAGAGCTCCT CACTGGACTC CAGTACCTCT 
AGCCAGGAGC ATCTGAACCA TTCCTCTAAG TCGGTCACCC CTGCTTCCAC ACTGACCAAA 
AGTGGCCCTG GCCGTTGGAA AACACCAGCA GCCATACCGG CCACCCCTGT GGCCGTCTCC 
CAGCCAATCC GAACAGACCT GCCTCCGCCA CCCCCGCCAC CTCCAGTCCA CTATGCCGGT 
GATTTCGATG GAATGTCCAT GGATTTGCCT CTCCCACCAC CCCCTTCCGC CAACCAGATA 
GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT GAACGGAGAA AGAGAGAAGA ACATCAGCGT 
TGGTATGAGA AGGAGAAGGC CCGCCTGGAG GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG 
GAGAGGAAGT TGGGCCAGAT GCGCACTCAG TCCTTAAACC CTGCTCCGTT TTCTCCCCTG 
ACTGCACAGC AGATGAAGCC CGAAAAGCCT TCCACACTCC AGCGGCCACA GGAAACAGTC 
ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC CGCACGATCG AGCGCAGAGA CTTGCAGTAC 
ATTACAGTCA GCAAAGAGGA GCTTTCCTCG GGGGACAGTC TGTCCCCCGA CCCGTGGAAG 
CGGGACGCCA AGGAGAAGCT GGAGAAGCAG CAGCAGATGC ACATCGTGGA CATGCTGAGC 
AAGGAGATCC AGGAGCTCCA GAGCAAACCG GACCGCAGCG CCGAGGAGAG CGACCGGCTG 
CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG AAGAGACTCC AGGAGTCGAA GCAGAAGGAC 
GAAGATGACG AGGAGGAGGA GGACGATGAT GTGGACACCA TGCTGATCAT GCAGCGCCTG 
GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA 
GAGATGCGCA AGCGGGAAGC GGAAGACCGA GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG 
GAGGAGGAGC GAACAAAACG AGACGCTGAA GAAAAGAGGC GACAGGAAGA AGGGTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGACGAGG CGGCGCGCAG GTTGCTGGAG 
CCCGAGGCGC CCGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT 
GACGCCTGTC GGGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA 
AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT 
CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG 
AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193937.1
CDS	143..5374
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	NM_001207008.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001207008.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG 
CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGATGGAC GTCTAGCTGC AGGTGATCAG 
CTCCTCAGTG TGGATGGACG AAGTCTGGTT GGACTCTCTC AGGAAAGGGC GGCAGAACTC 
ATGACAAGAA CAAGCTCTGT GGTGACACTG GAAGTAGCAA AGCAGGGTGC CATCTACCAC 
GGTCTGGCCA CCCTTCTCAA TCAGCCATCC CCCATGATGC AGAGAATTTC AGATCGTCGT 
GGCTCAGGTA AACCCCGACC AAAGAGTGAA GGCTTTGAGC TCTATAATAA TTCAACTCAA 
AATGGGTCTC CTGAGAGTCC TCAGCTGCCT TGGGCAGAAT ATAGTGAACC AAAGAAATTG 
CCTGGTGATG ACAGACTGAT GAAAAATAGA GCTGATCACC GTTCCAGCCC CAACGTAGCA 
AATCAGCCTC CTAGTCCTGG AGGGAAAAGT GCATATGCCT CTGGAACAAC AGCGAAGATA 
ACATCTGTCT CTACTGGAAA CCTCTGCACT GAGGAGCAGA CGCCTCCGCC TAGACCTGAA 
GCCTACCCCA TCCCCACTCA GACGTACACC AGAGAGTATT TTACCTTCCC AGCTTCCAAA 
TCCCAGGATC GGATGGCTCC TCCTCAGAAC CAGTGGCCAA ATTATGAGGA AAAGCCACAT 
ATGCACACAG ATAGTAATCA TTCCAGTATT GCAATTCAGC GTGTTACACG TTCCCAAGAA 
GAACTTCGAG AAGATAAAGC TTACCAACTT GAGCGGCATC GAATAGAGGC AGCTATGGAC 
CGAAAGTCTG ATAGTGATAT GTGGATAAAT CAGAGCTCCT CACTGGACTC CAGTACCTCT 
AGCCAGGAGC ATCTGAACCA TTCCTCTAAG TCGGTCACCC CTGCTTCCAC ACTGACCAAA 
AGTGGCCCTG GCCGTTGGAA AACACCAGCA GCCATACCGG CCACCCCTGT GGCCGTCTCC 
CAGCCAATCC GAACAGACCT GCCTCCGCCA CCCCCGCCAC CTCCAGTCCA CTATGCCGGT 
GATTTCGATG GAATGTCCAT GGATTTGCCT CTCCCACCAC CCCCTTCCGC CAACCAGATA 
GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT GAACGGAGAA AGAGAGAAGA ACATCAGCGT 
TGGTATGAGA AGGAGAAGGC CCGCCTGGAG GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG 
GAGAGGAAGT TGGGCCAGAT GCGCACTCAG TCCTTAAACC CTGCTCCGTT TTCTCCCCTG 
ACTGCACAGC AGATGAAGCC CGAAAAGCCT TCCACACTCC AGCGGCCACA GGAAACAGTC 
ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC CGCACGATCG AGCGCAGAGA CTTGCAGTAC 
ATTACAGTCA GCAAAGAGGA GCTTTCCTCG GGGGACAGTC TGTCCCCCGA CCCGTGGAAG 
CGGGACGCCA AGGAGAAGCT GGAGAAGCAG CAGCAGATGC ACATCGTGGA CATGCTGAGC 
AAGGAGATCC AGGAGCTCCA GAGCAAACCG GACCGCAGCG CCGAGGAGAG CGACCGGCTG 
CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG AAGAGACTCC AGGAGTCGAA GCAGAAGGAC 
GAAGATGACG AGGAGGAGGA GGACGATGAT GTGGACACCA TGCTGATCAT GCAGCGCCTG 
GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA 
GAGATGCGCA AGCGGGAAGC GGAAGACCGA GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG 
GAGGAGGAGC GAACAAAACG AGACGCTGAA GAAAAGAGGC GACAGGAAGA AGGGTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGACGAGG CGGCGCGCAG GTTGCTGGAG 
CCCGAGGCGC CCGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT 
GACGCCTGTC GGGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA 
AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT 
CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG 
AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_006715491.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715554.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

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CloneID	OHu40829
Clone ID Related Accession (Same CDS sequence)	XM_006715491.2
Accession Version	XM_006715491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006715491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAGCCCGA GGCGCCCGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA 
GCTGTTGGAG CCCATGACGC CTGTCGGGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715554.1
CDS	257..5563
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_006715491.2
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715491.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAGCCCGA GGCGCCCGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA 
GCTGTTGGAG CCCATGACGC CTGTCGGGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_006715494.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715557.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

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CloneID	OHu40832
Clone ID Related Accession (Same CDS sequence)	XM_006715494.3
Accession Version	XM_006715494.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006715494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG 
CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCGGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCA 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAGG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC CACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CGCCAACCAG 
ATAGGGCTGC CGTCTGCGCA GGTGGCTGCT GCTGAACGGA GAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATTACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGACGAT GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA 
GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG 
GAGCCCGAGG CGCCCGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715557.1
CDS	245..5479
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_006715494.3
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715494.3

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG 
CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCGGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCA 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAGG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC CACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CGCCAACCAG 
ATAGGGCTGC CGTCTGCGCA GGTGGCTGCT GCTGAACGGA GAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATTACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGACGAT GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA 
GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG 
GAGCCCGAGG CGCCCGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_011535842.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534144.1 Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.

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CloneID	OHu59006
Clone ID Related Accession (Same CDS sequence)	XM_011535842.2
Accession Version	XM_011535842.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAGCCCGA GGCGCCCGGA 
CCAAACTCTT ACCCAGGATC TACTGGAGCA GCTGTTGGAG CCCATGACGC CTGTCGGGAT 
GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT GCAGATTCAC CTGGAAGTTC TGGGGCCCCT 
GAAAACTTGA CATTCAAGGA ACGCCAGCGT CTTTTTTCAC AAGGTCAAGA TGTATCCAAT 
AAAGTGAAAG CTTCTCGTAA ATTAACAGAA CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534144.1
CDS	262..5538
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_011535842.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535842.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAGCCCGA GGCGCCCGGA 
CCAAACTCTT ACCCAGGATC TACTGGAGCA GCTGTTGGAG CCCATGACGC CTGTCGGGAT 
GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT GCAGATTCAC CTGGAAGTTC TGGGGCCCCT 
GAAAACTTGA CATTCAAGGA ACGCCAGCGT CTTTTTTCAC AAGGTCAAGA TGTATCCAAT 
AAAGTGAAAG CTTCTCGTAA ATTAACAGAA CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( NM_001366320.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353249.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 6, mRNA.

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CloneID	OHu40825
Clone ID Related Accession (Same CDS sequence)	XM_005266996.3 , NM_001366320.1
Accession Version	NM_001366320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	afadin isoform 6
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AL009178.4, AL731868.5 and AL049698.3. On Sep 29, 2018 this sequence version replaced XM_005266996.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353249.1
CDS	570..6044
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	NM_001366320.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366320.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( NM_001366321.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353250.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 7, mRNA.

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CloneID	OHu96620
Clone ID Related Accession (Same CDS sequence)	NM_001366321.2 , XM_017010883.1 , NM_001366321.1
Accession Version	NM_001366321.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	afadin isoform 7
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AL009178.4, AL731868.5 and AL049698.3. On Sep 29, 2018 this sequence version replaced XM_017010883.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT 
TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT 
GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353250.1
CDS	570..6023
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	NM_001366321.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366321.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT 
TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT 
GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_005266996.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267053.1 Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.

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CloneID	OHu40825
Clone ID Related Accession (Same CDS sequence)	XM_005266996.3 , NM_001366320.1
Accession Version	XM_005266996.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266996.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267053.1
CDS	262..5736
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_005266996.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005266996.3

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG 
TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_005266997.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267054.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

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CloneID	OHu40826
Clone ID Related Accession (Same CDS sequence)	XM_005266997.2
Accession Version	XM_005266997.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAACG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCGATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCATT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCGGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC ACATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAG 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CCACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCGT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCGCCAACCA GATAGGGCTG 
CCGTCTGCGC AGGTGGCTGC TGCTGAACGG AGAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATTACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGACGA TGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAGCC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT 
GACGCCTGTC GGGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA 
AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT 
CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG 
AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267054.1
CDS	257..5728
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_005266997.2
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005266997.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAACG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCGATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCATT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCGGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC ACATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAG 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CCACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCGT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCGCCAACCA GATAGGGCTG 
CCGTCTGCGC AGGTGGCTGC TGCTGAACGG AGAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATTACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGACGA TGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAGCC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT 
GACGCCTGTC GGGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA 
AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT 
CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG 
AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017010878.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866367.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

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CloneID	OHu96615
Clone ID Related Accession (Same CDS sequence)	XM_017010878.1
Accession Version	XM_017010878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAGCCCGA GGCGCCCGGT CTGTGCCGCC CTCCGCTTCC CCGGGACTAC 
GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC GCCCCTCCTC CCCCGCCTCA GCGAAACGCC 
TCCTACCTCA AAACACAGGT CCTCTCCCCC GACTCGCTGT TCACTGCCAA GTTTGTTGCA 
TACAATGAGG AGGAGGAGGA GGAGGACTGC AGCCTAGCAG GACCAAACTC TTACCCAGGA 
TCTACTGGAG CAGCTGTTGG AGCCCATGAC GCCTGTCGGG ATGCAAAAGA GAAGCGCTCT 
AAAAGCCAAG ATGCAGATTC ACCTGGAAGT TCTGGGGCCC CTGAAAACTT GACATTCAAG 
GAACGCCAGC GTCTTTTTTC ACAAGGTCAA GATGTATCCA ATAAAGTGAA AGCTTCTCGT 
AAATTAACAG AACTGGAGAA TGAACTGAAC ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866367.1
CDS	257..5755
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017010878.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010878.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAGCCCGA GGCGCCCGGT CTGTGCCGCC CTCCGCTTCC CCGGGACTAC 
GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC GCCCCTCCTC CCCCGCCTCA GCGAAACGCC 
TCCTACCTCA AAACACAGGT CCTCTCCCCC GACTCGCTGT TCACTGCCAA GTTTGTTGCA 
TACAATGAGG AGGAGGAGGA GGAGGACTGC AGCCTAGCAG GACCAAACTC TTACCCAGGA 
TCTACTGGAG CAGCTGTTGG AGCCCATGAC GCCTGTCGGG ATGCAAAAGA GAAGCGCTCT 
AAAAGCCAAG ATGCAGATTC ACCTGGAAGT TCTGGGGCCC CTGAAAACTT GACATTCAAG 
GAACGCCAGC GTCTTTTTTC ACAAGGTCAA GATGTATCCA ATAAAGTGAA AGCTTCTCGT 
AAATTAACAG AACTGGAGAA TGAACTGAAC ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017010879.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866368.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

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CloneID	OHu96616
Clone ID Related Accession (Same CDS sequence)	XM_017010879.1
Accession Version	XM_017010879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAACGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CGATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCATTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCGGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCAC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAGGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCC 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCGTCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
GCCAACCAGA TAGGGCTGCC GTCTGCGCAG GTGGCTGCTG CTGAACGGAG AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATTACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGACGATG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGTTGCAGG ACGAGGAGCG GAGGCGGCAG 
CAGCAGTTAG AAGAGATGCG CAAGCGGGAA GCGGAAGACC GAGCGAGGCA AGAGGAAGAG 
CGCCGGCGGC AGGAGGAGGA GCGAACAAAA CGAGACGCTG AAGAAAAGAG GCGACAGGAA 
GAAGGGTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCACGACGA GGCGGCGCGC 
AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG TGCCGCCCTC CGCTTCCCCG GGACTACGAG 
CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC CCTCCTCCCC CGCCTCAGCG AAACGCCTCC 
TACCTCAAAA CACAGGTCCT CTCCCCCGAC TCGCTGTTCA CTGCCAAGTT TGTTGCATAC 
AATGAGGAGG AGGAGGAGGA GGACTGCAGC CTAGCAGGAC CAAACTCTTA CCCAGGATCT 
ACTGGAGCAG CTGTTGGAGC CCATGACGCC TGTCGGGATG CAAAAGAGAA GCGCTCTAAA 
AGCCAAGATG CAGATTCACC TGGAAGTTCT GGGGCCCCTG AAAACTTGAC ATTCAAGGAA 
CGCCAGCGTC TTTTTTCACA AGGTCAAGAT GTATCCAATA AAGTGAAAGC TTCTCGTAAA 
TTAACAGAAC TGGAGAATGA ACTGAACACA AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866368.1
CDS	262..5757
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017010879.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010879.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAACGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CGATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCATTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCGGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCAC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAGGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCC 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCGTCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
GCCAACCAGA TAGGGCTGCC GTCTGCGCAG GTGGCTGCTG CTGAACGGAG AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATTACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGACGATG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGTTGCAGG ACGAGGAGCG GAGGCGGCAG 
CAGCAGTTAG AAGAGATGCG CAAGCGGGAA GCGGAAGACC GAGCGAGGCA AGAGGAAGAG 
CGCCGGCGGC AGGAGGAGGA GCGAACAAAA CGAGACGCTG AAGAAAAGAG GCGACAGGAA 
GAAGGGTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCACGACGA GGCGGCGCGC 
AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG TGCCGCCCTC CGCTTCCCCG GGACTACGAG 
CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC CCTCCTCCCC CGCCTCAGCG AAACGCCTCC 
TACCTCAAAA CACAGGTCCT CTCCCCCGAC TCGCTGTTCA CTGCCAAGTT TGTTGCATAC 
AATGAGGAGG AGGAGGAGGA GGACTGCAGC CTAGCAGGAC CAAACTCTTA CCCAGGATCT 
ACTGGAGCAG CTGTTGGAGC CCATGACGCC TGTCGGGATG CAAAAGAGAA GCGCTCTAAA 
AGCCAAGATG CAGATTCACC TGGAAGTTCT GGGGCCCCTG AAAACTTGAC ATTCAAGGAA 
CGCCAGCGTC TTTTTTCACA AGGTCAAGAT GTATCCAATA AAGTGAAAGC TTCTCGTAAA 
TTAACAGAAC TGGAGAATGA ACTGAACACA AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017010880.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866369.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

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CloneID	OHu96617
Clone ID Related Accession (Same CDS sequence)	XM_017010880.1
Accession Version	XM_017010880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAACGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CGATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCATTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCGGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCAC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAGGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCC 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCGTCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
GCCAACCAGA TAGGGCTGCC GTCTGCGCAG GTGGCTGCTG CTGAACGGAG AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATTACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGACGATG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGTTGCAGG ACGAGGAGCG GAGGCGGCAG 
CAGCAGTTAG AAGAGATGCG CAAGCGGGAA GCGGAAGACC GAGCGAGGCA AGAGGAAGAG 
CGCCGGCGGC AGGAGGAGGA GCGAACAAAA CGAGACGCTG AAGAAAAGAG GCGACAGGAA 
GAAGGGTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCACGACGA GGCGGCGCGC 
AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG TGCCGCCCTC CGCTTCCCCG GGACTACGAG 
CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC CCTCCTCCCC CGCCTCAGCG AAACGCCTCC 
TACCTCAAAA CACAGGTCCT CTCCCCCGAC TCGCTGTTCA CTGCCAAGTT TGTTGCATAC 
AATGAGGAGG AGGAGGAGGA GGACTGCAGC CTAGCAGGAC CAAACTCTTA CCCAGGATCT 
ACTGGAGCAG CTGTTGGAGC CCATGACGCC TGTCGGGATG CAAAAGAGAA GCGCTCTAAA 
AGCCAAGATG CAGATTCACC TGGAAGTTCT GGGGCCCCTG AAAACTTGAC ATTCAAGGAA 
CGCCAGCGTC TTTTTTCACA AGGTCAAGAT GTATCCAATA AAGTGAAAGC TTCTCGTAAA 
TTAACAGAAC TGGAGAATGA ACTGAACACA AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866369.1
CDS	241..5736
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017010880.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010880.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAACGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CGATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCATTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCGGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCAC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAGGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCC 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCGTCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
GCCAACCAGA TAGGGCTGCC GTCTGCGCAG GTGGCTGCTG CTGAACGGAG AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATTACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGACGATG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGTTGCAGG ACGAGGAGCG GAGGCGGCAG 
CAGCAGTTAG AAGAGATGCG CAAGCGGGAA GCGGAAGACC GAGCGAGGCA AGAGGAAGAG 
CGCCGGCGGC AGGAGGAGGA GCGAACAAAA CGAGACGCTG AAGAAAAGAG GCGACAGGAA 
GAAGGGTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCACGACGA GGCGGCGCGC 
AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG TGCCGCCCTC CGCTTCCCCG GGACTACGAG 
CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC CCTCCTCCCC CGCCTCAGCG AAACGCCTCC 
TACCTCAAAA CACAGGTCCT CTCCCCCGAC TCGCTGTTCA CTGCCAAGTT TGTTGCATAC 
AATGAGGAGG AGGAGGAGGA GGACTGCAGC CTAGCAGGAC CAAACTCTTA CCCAGGATCT 
ACTGGAGCAG CTGTTGGAGC CCATGACGCC TGTCGGGATG CAAAAGAGAA GCGCTCTAAA 
AGCCAAGATG CAGATTCACC TGGAAGTTCT GGGGCCCCTG AAAACTTGAC ATTCAAGGAA 
CGCCAGCGTC TTTTTTCACA AGGTCAAGAT GTATCCAATA AAGTGAAAGC TTCTCGTAAA 
TTAACAGAAC TGGAGAATGA ACTGAACACA AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017010881.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866370.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

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CloneID	OHu96618
Clone ID Related Accession (Same CDS sequence)	XM_017010881.1
Accession Version	XM_017010881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT 
CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT 
GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG 
TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG 
CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGCTT CTGGGCCTGA GCTGATACTA 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT 
AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGTGTCAT CCAGGAAGTA 
GACCAGGTTG ACCAGAAACA GAAGAATATT GCAGGGGCAC TTGCCTTCTG GATGGCAAAT 
GCATCTGAAC TTCTCAACTT CATTAAGCAA GACCGAGACC TTAGTCGGAT CACACTGGAT 
GCTCAAGATG TTTTAGCACA TTTGGTTCAA ATGGCATTTA AATACTTGGT TCACTGTCTT 
CAATCAGAAC TTAATAATTA CATGCCAGCC TTTCTAGATG ACCCTGAAGA GAACAGTCTG 
CAACGACCAA AAATAGATGA TGTGCTGCAC ACGCTCACAG GAGCCATGTC CTTGCTACGA 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTCATC 
AATATGTGGC TGTTCAATAG ATTGGTGACC GACCCAGATT CGGGGCTGTG CTCCCATTAC 
TGGGGTGCGA TTATCCGTCA GCAGTTGGGC CATATTGAAG CCTGGGCTGA GAAGCAGGGG 
CTGGAACTGG CTGCGGACTG TCATCTGAGC AGGATCGTGC AGGCAACGAC TTTGCTTACC 
ATGGATAAGT ATGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT TAAGTTAAAT 
TCATTACAAC TTCAAGCCTT ATTACAGAAC TATCACTGTG CACCTGATGA GCCTTTTATC 
CCAACGGATC TTATAGAAAA TGTAGTGACT GTGGCTGAAA ACACTGCCGA TGAGCTGGCC 
CGCAGTGATG GAAGGGAAGT GCAGTTGGAG GAGGATCCTG ATCTGCAGCT GCCGTTTCTT 
TTGCCAGAAG ATGGTTATTC TTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA 
TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGCAGGTTAA TTCCTCACAC ACGTTCACCA 
GGTACTTGGA CAATATATTT TGAAGGTGCA GATTATGAAA GTCACCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC CCTAAAAAAG 
CAGAATGGAA TGGGCCTTAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTAGGA 
ATCTATGTGA AGTCGGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA 
GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA 
AAGCCACATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA 
GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC 
AGTACCTCTA GCCAGGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCCACA 
CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCGTCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCGCC 
AACCAGATAG GGCTGCCGTC TGCGCAGGTG GCTGCTGCTG AACGGAGAAA GAGAGAAGAA 
CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT 
TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG 
GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC 
TTGCAGTACA TTACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC 
CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGATGACGA GGAGGAGGAG GACGATGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG TTGCAGGACG AGGAGCGGAG GCGGCAGCAG 
CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC 
CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG 
TTGCTGGAGC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC 
CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT 
GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGACCAA ACTCTTACCC AGGATCTACT 
GGAGCAGCTG TTGGAGCCCA TGACGCCTGT CGGGATGCAA AAGAGAAGCG CTCTAAAAGC 
CAAGATGCAG ATTCACCTGG AAGTTCTGGG GCCCCTGAAA ACTTGACATT CAAGGAACGC 
CAGCGTCTTT TTTCACAAGG TCAAGATGTA TCCAATAAAG TGAAAGCTTC TCGTAAATTA 
ACAGAACTGG AGAATGAACT GAACACAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866370.1
CDS	245..5737
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017010881.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010881.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT 
CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT 
GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG 
TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG 
CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGCTT CTGGGCCTGA GCTGATACTA 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT 
AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGTGTCAT CCAGGAAGTA 
GACCAGGTTG ACCAGAAACA GAAGAATATT GCAGGGGCAC TTGCCTTCTG GATGGCAAAT 
GCATCTGAAC TTCTCAACTT CATTAAGCAA GACCGAGACC TTAGTCGGAT CACACTGGAT 
GCTCAAGATG TTTTAGCACA TTTGGTTCAA ATGGCATTTA AATACTTGGT TCACTGTCTT 
CAATCAGAAC TTAATAATTA CATGCCAGCC TTTCTAGATG ACCCTGAAGA GAACAGTCTG 
CAACGACCAA AAATAGATGA TGTGCTGCAC ACGCTCACAG GAGCCATGTC CTTGCTACGA 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTCATC 
AATATGTGGC TGTTCAATAG ATTGGTGACC GACCCAGATT CGGGGCTGTG CTCCCATTAC 
TGGGGTGCGA TTATCCGTCA GCAGTTGGGC CATATTGAAG CCTGGGCTGA GAAGCAGGGG 
CTGGAACTGG CTGCGGACTG TCATCTGAGC AGGATCGTGC AGGCAACGAC TTTGCTTACC 
ATGGATAAGT ATGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT TAAGTTAAAT 
TCATTACAAC TTCAAGCCTT ATTACAGAAC TATCACTGTG CACCTGATGA GCCTTTTATC 
CCAACGGATC TTATAGAAAA TGTAGTGACT GTGGCTGAAA ACACTGCCGA TGAGCTGGCC 
CGCAGTGATG GAAGGGAAGT GCAGTTGGAG GAGGATCCTG ATCTGCAGCT GCCGTTTCTT 
TTGCCAGAAG ATGGTTATTC TTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA 
TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGCAGGTTAA TTCCTCACAC ACGTTCACCA 
GGTACTTGGA CAATATATTT TGAAGGTGCA GATTATGAAA GTCACCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC CCTAAAAAAG 
CAGAATGGAA TGGGCCTTAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTAGGA 
ATCTATGTGA AGTCGGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA 
GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA 
AAGCCACATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA 
GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC 
AGTACCTCTA GCCAGGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCCACA 
CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCGTCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCGCC 
AACCAGATAG GGCTGCCGTC TGCGCAGGTG GCTGCTGCTG AACGGAGAAA GAGAGAAGAA 
CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT 
TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG 
GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC 
TTGCAGTACA TTACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC 
CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGATGACGA GGAGGAGGAG GACGATGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG TTGCAGGACG AGGAGCGGAG GCGGCAGCAG 
CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC 
CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG 
TTGCTGGAGC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC 
CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT 
GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGACCAA ACTCTTACCC AGGATCTACT 
GGAGCAGCTG TTGGAGCCCA TGACGCCTGT CGGGATGCAA AAGAGAAGCG CTCTAAAAGC 
CAAGATGCAG ATTCACCTGG AAGTTCTGGG GCCCCTGAAA ACTTGACATT CAAGGAACGC 
CAGCGTCTTT TTTCACAAGG TCAAGATGTA TCCAATAAAG TGAAAGCTTC TCGTAAATTA 
ACAGAACTGG AGAATGAACT GAACACAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017010882.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866371.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

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CloneID	OHu96619
Clone ID Related Accession (Same CDS sequence)	XM_017010882.1
Accession Version	XM_017010882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAACG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCGATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCATT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCGGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC ACATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAG 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CCACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCGT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCGCCAACCA GATAGGGCTG 
CCGTCTGCGC AGGTGGCTGC TGCTGAACGG AGAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATTACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGACGA TGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGTTGCA GGACGAGGAG CGGAGGCGGC AGCAGCAGTT AGAAGAGATG 
CGCAAGCGGG AAGCGGAAGA CCGAGCGAGG CAAGAGGAAG AGCGCCGGCG GCAGGAGGAG 
GAGCGAACAA AACGAGACGC TGAAGAAAAG AGGCGACAGG AAGAAGGGTA TTACAGCCGC 
CTGGAAGCCG AGAGGCGCAG ACAGCACGAC GAGGCGGCGC GCAGGTTGCT GGAGCCCGAG 
GCGCCCGGTC TGTGCCGCCC TCCGCTTCCC CGGGACTACG AGCCCCCGTC CCCGTCCCCC 
GCGCCCGGCG CCCCTCCTCC CCCGCCTCAG CGAAACGCCT CCTACCTCAA AACACAGGTC 
CTCTCCCCCG ACTCGCTGTT CACTGCCAAG TTTGTTGCAT ACAATGAGGA GGAGGAGGAG 
GAGGACTGCA GCCTAGCAGG ACCAAACTCT TACCCAGGAT CTACTGGAGC AGCTGTTGGA 
GCCCATGACG CCTGTCGGGA TGCAAAAGAG AAGCGCTCTA AAAGCCAAGA TGCAGATTCA 
CCTGGAAGTT CTGGGGCCCC TGAAAACTTG ACATTCAAGG AACGCCAGCG TCTTTTTTCA 
CAAGGTCAAG ATGTATCCAA TAAAGTGAAA GCTTCTCGTA AATTAACAGA ACTGGAGAAT 
GAACTGAACA CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866371.1
CDS	257..5734
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017010882.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010882.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAACG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCGATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCATT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCGGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC ACATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAG 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CCACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCGT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCGCCAACCA GATAGGGCTG 
CCGTCTGCGC AGGTGGCTGC TGCTGAACGG AGAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATTACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGACGA TGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGTTGCA GGACGAGGAG CGGAGGCGGC AGCAGCAGTT AGAAGAGATG 
CGCAAGCGGG AAGCGGAAGA CCGAGCGAGG CAAGAGGAAG AGCGCCGGCG GCAGGAGGAG 
GAGCGAACAA AACGAGACGC TGAAGAAAAG AGGCGACAGG AAGAAGGGTA TTACAGCCGC 
CTGGAAGCCG AGAGGCGCAG ACAGCACGAC GAGGCGGCGC GCAGGTTGCT GGAGCCCGAG 
GCGCCCGGTC TGTGCCGCCC TCCGCTTCCC CGGGACTACG AGCCCCCGTC CCCGTCCCCC 
GCGCCCGGCG CCCCTCCTCC CCCGCCTCAG CGAAACGCCT CCTACCTCAA AACACAGGTC 
CTCTCCCCCG ACTCGCTGTT CACTGCCAAG TTTGTTGCAT ACAATGAGGA GGAGGAGGAG 
GAGGACTGCA GCCTAGCAGG ACCAAACTCT TACCCAGGAT CTACTGGAGC AGCTGTTGGA 
GCCCATGACG CCTGTCGGGA TGCAAAAGAG AAGCGCTCTA AAAGCCAAGA TGCAGATTCA 
CCTGGAAGTT CTGGGGCCCC TGAAAACTTG ACATTCAAGG AACGCCAGCG TCTTTTTTCA 
CAAGGTCAAG ATGTATCCAA TAAAGTGAAA GCTTCTCGTA AATTAACAGA ACTGGAGAAT 
GAACTGAACA CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017010883.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866372.1 Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.

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CloneID	OHu96620
Clone ID Related Accession (Same CDS sequence)	NM_001366321.2 , XM_017010883.1 , NM_001366321.1
Accession Version	XM_017010883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT 
TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT 
GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866372.1
CDS	257..5710
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017010883.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010883.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT 
TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT 
GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017010884.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866373.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

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CloneID	OHu96621
Clone ID Related Accession (Same CDS sequence)	XM_017010884.1
Accession Version	XM_017010884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGATG GTTCTGACTC TAGAGATAAG 
CCAAAGCTTT ACCGCCTTCA GTTAAGTGTT ACTGAAGTTG GGACAGAAAA GTTGGATGAC 
AACTCTATCC AGTTGTTTGG CCCAGGAATT CAGCCCCATC ACTGTGACCT TACCAACATG 
GATGGAGTGG TCACTGTGAC GCCCAGAAGT ATGGACGCAG AAACCTACGT GGAAGGCCAG 
CGCATCTCAG AAACCACCAT GCTGCAGAGT GGCATGAAAG TGCAGTTTGG GGCGTCCCAT 
GTATTTAAGT TTGTGGACCC CAGTCAGGAT CATGCTCTTG CAAAAAGATC TGTGGATGGA 
GGCCTGATGG TTAAGGGCCC AAGACATAAA CCTGGAATTG TTCAGGAGAC AACTTTTGAT 
TTGGGAGGAG ATATTCATAG TGGGACAGCA TTACCGACAA GCAAGAGCAC CACTAGGCTG 
GACAGCGACA GAGTGTCGTC TGCCTCTAGC ACAGCCGAGC GGGGAATGGT GAAGCCGATG 
ATCAGAGTAG AACAGCAGCC AGATTATCGC AGGCAAGAAA GCAGAACACA GGATGCTTCT 
GGGCCTGAGC TGATACTACC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCATTT 
TTGTCTGCCA TTATAAATTA TACTAATAGC TCTACAGTCC ACTTTAAGTT GTCCCCTACA 
TATGTATTAT ATATGGCATG CCGGTATGTA TTGTCCAACC AGTACAGACC TGACATCAGC 
CCTACAGAGC GCACACATAA AGTCATTGCA GTCGTCAACA AGATGGTGAG CATGATGGAG 
GGTGTCATCC AGGAAGTAGA CCAGGTTGAC CAGAAACAGA AGAATATTGC AGGGGCACTT 
GCCTTCTGGA TGGCAAATGC ATCTGAACTT CTCAACTTCA TTAAGCAAGA CCGAGACCTT 
AGTCGGATCA CACTGGATGC TCAAGATGTT TTAGCACATT TGGTTCAAAT GGCATTTAAA 
TACTTGGTTC ACTGTCTTCA ATCAGAACTT AATAATTACA TGCCAGCCTT TCTAGATGAC 
CCTGAAGAGA ACAGTCTGCA ACGACCAAAA ATAGATGATG TGCTGCACAC GCTCACAGGA 
GCCATGTCCT TGCTACGACG CTGCAGAGTC AATGCCGCCC TGACCATCCA GCTCTTCTCT 
CAGCTCTTCC ACTTCATCAA TATGTGGCTG TTCAATAGAT TGGTGACCGA CCCAGATTCG 
GGGCTGTGCT CCCATTACTG GGGTGCGATT ATCCGTCAGC AGTTGGGCCA TATTGAAGCC 
TGGGCTGAGA AGCAGGGGCT GGAACTGGCT GCGGACTGTC ATCTGAGCAG GATCGTGCAG 
GCAACGACTT TGCTTACCAT GGATAAGTAT GCACCTGATG ACATTCCAAA TATAAACAGC 
ACCTGCTTTA AGTTAAATTC ATTACAACTT CAAGCCTTAT TACAGAACTA TCACTGTGCA 
CCTGATGAGC CTTTTATCCC AACGGATCTT ATAGAAAATG TAGTGACTGT GGCTGAAAAC 
ACTGCCGATG AGCTGGCCCG CAGTGATGGA AGGGAAGTGC AGTTGGAGGA GGATCCTGAT 
CTGCAGCTGC CGTTTCTTTT GCCAGAAGAT GGTTATTCTT GTGATGTTGT CAGAAACATT 
CCAAATGGTT TACAAGAATT TTTAGACCCT CTGTGCCAGA GAGGATTTTG CAGGTTAATT 
CCTCACACAC GTTCACCAGG TACTTGGACA ATATATTTTG AAGGTGCAGA TTATGAAAGT 
CACCTTCTGC GTGAGAACAC AGAGCTGGCT CAGCCTCTGA GGAAAGAACC TGAAATAATC 
ACTGTGACCC TAAAAAAGCA GAATGGAATG GGCCTTAGCA TTGTTGCAGC AAAGGGTGCT 
GGTCAAGATA AACTAGGAAT CTATGTGAAG TCGGTTGTGA AAGGAGGTGC TGCAGATGTG 
GATGGACGTC TAGCTGCAGG TGATCAGCTC CTCAGTGTGG ATGGACGAAG TCTGGTTGGA 
CTCTCTCAGG AAAGGGCGGC AGAACTCATG ACAAGAACAA GCTCTGTGGT GACACTGGAA 
GTAGCAAAGC AGGGTGCCAT CTACCACGGT CTGGCCACCC TTCTCAATCA GCCATCCCCC 
ATGATGCAGA GAATTTCAGA TCGTCGTGGC TCAGGTAAAC CCCGACCAAA GAGTGAAGGC 
TTTGAGCTCT ATAATAATTC AACTCAAAAT GGGTCTCCTG AGAGTCCTCA GCTGCCTTGG 
GCAGAATATA GTGAACCAAA GAAATTGCCT GGTGATGACA GACTGATGAA AAATAGAGCT 
GATCACCGTT CCAGCCCCAA CGTAGCAAAT CAGCCTCCTA GTCCTGGAGG GAAAAGTGCA 
TATGCCTCTG GAACAACAGC GAAGATAACA TCTGTCTCTA CTGGAAACCT CTGCACTGAG 
GAGCAGACGC CTCCGCCTAG ACCTGAAGCC TACCCCATCC CCACTCAGAC GTACACCAGA 
GAGTATTTTA CCTTCCCAGC TTCCAAATCC CAGGATCGGA TGGCTCCTCC TCAGAACCAG 
TGGCCAAATT ATGAGGAAAA GCCACATATG CACACAGATA GTAATCATTC CAGTATTGCA 
ATTCAGCGTG TTACACGTTC CCAAGAAGAA CTTCGAGAAG ATAAAGCTTA CCAACTTGAG 
CGGCATCGAA TAGAGGCAGC TATGGACCGA AAGTCTGATA GTGATATGTG GATAAATCAG 
AGCTCCTCAC TGGACTCCAG TACCTCTAGC CAGGAGCATC TGAACCATTC CTCTAAGTCG 
GTCACCCCTG CTTCCACACT GACCAAAAGT GGCCCTGGCC GTTGGAAAAC ACCAGCAGCC 
ATACCGGCCA CCCCTGTGGC CGTCTCCCAG CCAATCCGAA CAGACCTGCC TCCGCCACCC 
CCGCCACCTC CAGTCCACTA TGCCGGTGAT TTCGATGGAA TGTCCATGGA TTTGCCTCTC 
CCACCACCCC CTTCCGCCAA CCAGATAGGG CTGCCGTCTG CGCAGGTGGC TGCTGCTGAA 
CGGAGAAAGA GAGAAGAACA TCAGCGTTGG TATGAGAAGG AGAAGGCCCG CCTGGAGGAG 
GAGCGGGAGA GGAAGCGGAG AGAGCAGGAG AGGAAGTTGG GCCAGATGCG CACTCAGTCC 
TTAAACCCTG CTCCGTTTTC TCCCCTGACT GCACAGCAGA TGAAGCCCGA AAAGCCTTCC 
ACACTCCAGC GGCCACAGGA AACAGTCATT CGGGAGCTGC AGCCTCAGCA GCAGCCCCGC 
ACGATCGAGC GCAGAGACTT GCAGTACATT ACAGTCAGCA AAGAGGAGCT TTCCTCGGGG 
GACAGTCTGT CCCCCGACCC GTGGAAGCGG GACGCCAAGG AGAAGCTGGA GAAGCAGCAG 
CAGATGCACA TCGTGGACAT GCTGAGCAAG GAGATCCAGG AGCTCCAGAG CAAACCGGAC 
CGCAGCGCCG AGGAGAGCGA CCGGCTGCGC AAGCTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTCCAGG AGTCGAAGCA GAAGGACGAA GATGACGAGG AGGAGGAGGA CGATGATGTG 
GACACCATGC TGATCATGCA GCGCCTGGAG GCTGAACGAA GAGCGAGGTT GCAGGACGAG 
GAGCGGAGGC GGCAGCAGCA GTTAGAAGAG ATGCGCAAGC GGGAAGCGGA AGACCGAGCG 
AGGCAAGAGG AAGAGCGCCG GCGGCAGGAG GAGGAGCGAA CAAAACGAGA CGCTGAAGAA 
AAGAGGCGAC AGGAAGAAGG GTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC 
GACGAGGCGG CGCGCAGGTT GCTGGAGCCC GAGGCGCCCG GTCTGTGCCG CCCTCCGCTT 
CCCCGGGACT ACGAGCCCCC GTCCCCGTCC CCCGCGCCCG GCGCCCCTCC TCCCCCGCCT 
CAGCGAAACG CCTCCTACCT CAAAACACAG GTCCTCTCCC CCGACTCGCT GTTCACTGCC 
AAGTTTGTTG CATACAATGA GGAGGAGGAG GAGGAGGACT GCAGCCTAGC AGGACCAAAC 
TCTTACCCAG GATCTACTGG AGCAGCTGTT GGAGCCCATG ACGCCTGTCG GGATGCAAAA 
GAGAAGCGCT CTAAAAGCCA AGATGCAGAT TCACCTGGAA GTTCTGGGGC CCCTGAAAAC 
TTGACATTCA AGGAACGCCA GCGTCTTTTT TCACAAGGTC AAGATGTATC CAATAAAGTG 
AAAGCTTCTC GTAAATTAAC AGAACTGGAG AATGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866373.1
CDS	262..5712
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017010884.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010884.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGATG GTTCTGACTC TAGAGATAAG 
CCAAAGCTTT ACCGCCTTCA GTTAAGTGTT ACTGAAGTTG GGACAGAAAA GTTGGATGAC 
AACTCTATCC AGTTGTTTGG CCCAGGAATT CAGCCCCATC ACTGTGACCT TACCAACATG 
GATGGAGTGG TCACTGTGAC GCCCAGAAGT ATGGACGCAG AAACCTACGT GGAAGGCCAG 
CGCATCTCAG AAACCACCAT GCTGCAGAGT GGCATGAAAG TGCAGTTTGG GGCGTCCCAT 
GTATTTAAGT TTGTGGACCC CAGTCAGGAT CATGCTCTTG CAAAAAGATC TGTGGATGGA 
GGCCTGATGG TTAAGGGCCC AAGACATAAA CCTGGAATTG TTCAGGAGAC AACTTTTGAT 
TTGGGAGGAG ATATTCATAG TGGGACAGCA TTACCGACAA GCAAGAGCAC CACTAGGCTG 
GACAGCGACA GAGTGTCGTC TGCCTCTAGC ACAGCCGAGC GGGGAATGGT GAAGCCGATG 
ATCAGAGTAG AACAGCAGCC AGATTATCGC AGGCAAGAAA GCAGAACACA GGATGCTTCT 
GGGCCTGAGC TGATACTACC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCATTT 
TTGTCTGCCA TTATAAATTA TACTAATAGC TCTACAGTCC ACTTTAAGTT GTCCCCTACA 
TATGTATTAT ATATGGCATG CCGGTATGTA TTGTCCAACC AGTACAGACC TGACATCAGC 
CCTACAGAGC GCACACATAA AGTCATTGCA GTCGTCAACA AGATGGTGAG CATGATGGAG 
GGTGTCATCC AGGAAGTAGA CCAGGTTGAC CAGAAACAGA AGAATATTGC AGGGGCACTT 
GCCTTCTGGA TGGCAAATGC ATCTGAACTT CTCAACTTCA TTAAGCAAGA CCGAGACCTT 
AGTCGGATCA CACTGGATGC TCAAGATGTT TTAGCACATT TGGTTCAAAT GGCATTTAAA 
TACTTGGTTC ACTGTCTTCA ATCAGAACTT AATAATTACA TGCCAGCCTT TCTAGATGAC 
CCTGAAGAGA ACAGTCTGCA ACGACCAAAA ATAGATGATG TGCTGCACAC GCTCACAGGA 
GCCATGTCCT TGCTACGACG CTGCAGAGTC AATGCCGCCC TGACCATCCA GCTCTTCTCT 
CAGCTCTTCC ACTTCATCAA TATGTGGCTG TTCAATAGAT TGGTGACCGA CCCAGATTCG 
GGGCTGTGCT CCCATTACTG GGGTGCGATT ATCCGTCAGC AGTTGGGCCA TATTGAAGCC 
TGGGCTGAGA AGCAGGGGCT GGAACTGGCT GCGGACTGTC ATCTGAGCAG GATCGTGCAG 
GCAACGACTT TGCTTACCAT GGATAAGTAT GCACCTGATG ACATTCCAAA TATAAACAGC 
ACCTGCTTTA AGTTAAATTC ATTACAACTT CAAGCCTTAT TACAGAACTA TCACTGTGCA 
CCTGATGAGC CTTTTATCCC AACGGATCTT ATAGAAAATG TAGTGACTGT GGCTGAAAAC 
ACTGCCGATG AGCTGGCCCG CAGTGATGGA AGGGAAGTGC AGTTGGAGGA GGATCCTGAT 
CTGCAGCTGC CGTTTCTTTT GCCAGAAGAT GGTTATTCTT GTGATGTTGT CAGAAACATT 
CCAAATGGTT TACAAGAATT TTTAGACCCT CTGTGCCAGA GAGGATTTTG CAGGTTAATT 
CCTCACACAC GTTCACCAGG TACTTGGACA ATATATTTTG AAGGTGCAGA TTATGAAAGT 
CACCTTCTGC GTGAGAACAC AGAGCTGGCT CAGCCTCTGA GGAAAGAACC TGAAATAATC 
ACTGTGACCC TAAAAAAGCA GAATGGAATG GGCCTTAGCA TTGTTGCAGC AAAGGGTGCT 
GGTCAAGATA AACTAGGAAT CTATGTGAAG TCGGTTGTGA AAGGAGGTGC TGCAGATGTG 
GATGGACGTC TAGCTGCAGG TGATCAGCTC CTCAGTGTGG ATGGACGAAG TCTGGTTGGA 
CTCTCTCAGG AAAGGGCGGC AGAACTCATG ACAAGAACAA GCTCTGTGGT GACACTGGAA 
GTAGCAAAGC AGGGTGCCAT CTACCACGGT CTGGCCACCC TTCTCAATCA GCCATCCCCC 
ATGATGCAGA GAATTTCAGA TCGTCGTGGC TCAGGTAAAC CCCGACCAAA GAGTGAAGGC 
TTTGAGCTCT ATAATAATTC AACTCAAAAT GGGTCTCCTG AGAGTCCTCA GCTGCCTTGG 
GCAGAATATA GTGAACCAAA GAAATTGCCT GGTGATGACA GACTGATGAA AAATAGAGCT 
GATCACCGTT CCAGCCCCAA CGTAGCAAAT CAGCCTCCTA GTCCTGGAGG GAAAAGTGCA 
TATGCCTCTG GAACAACAGC GAAGATAACA TCTGTCTCTA CTGGAAACCT CTGCACTGAG 
GAGCAGACGC CTCCGCCTAG ACCTGAAGCC TACCCCATCC CCACTCAGAC GTACACCAGA 
GAGTATTTTA CCTTCCCAGC TTCCAAATCC CAGGATCGGA TGGCTCCTCC TCAGAACCAG 
TGGCCAAATT ATGAGGAAAA GCCACATATG CACACAGATA GTAATCATTC CAGTATTGCA 
ATTCAGCGTG TTACACGTTC CCAAGAAGAA CTTCGAGAAG ATAAAGCTTA CCAACTTGAG 
CGGCATCGAA TAGAGGCAGC TATGGACCGA AAGTCTGATA GTGATATGTG GATAAATCAG 
AGCTCCTCAC TGGACTCCAG TACCTCTAGC CAGGAGCATC TGAACCATTC CTCTAAGTCG 
GTCACCCCTG CTTCCACACT GACCAAAAGT GGCCCTGGCC GTTGGAAAAC ACCAGCAGCC 
ATACCGGCCA CCCCTGTGGC CGTCTCCCAG CCAATCCGAA CAGACCTGCC TCCGCCACCC 
CCGCCACCTC CAGTCCACTA TGCCGGTGAT TTCGATGGAA TGTCCATGGA TTTGCCTCTC 
CCACCACCCC CTTCCGCCAA CCAGATAGGG CTGCCGTCTG CGCAGGTGGC TGCTGCTGAA 
CGGAGAAAGA GAGAAGAACA TCAGCGTTGG TATGAGAAGG AGAAGGCCCG CCTGGAGGAG 
GAGCGGGAGA GGAAGCGGAG AGAGCAGGAG AGGAAGTTGG GCCAGATGCG CACTCAGTCC 
TTAAACCCTG CTCCGTTTTC TCCCCTGACT GCACAGCAGA TGAAGCCCGA AAAGCCTTCC 
ACACTCCAGC GGCCACAGGA AACAGTCATT CGGGAGCTGC AGCCTCAGCA GCAGCCCCGC 
ACGATCGAGC GCAGAGACTT GCAGTACATT ACAGTCAGCA AAGAGGAGCT TTCCTCGGGG 
GACAGTCTGT CCCCCGACCC GTGGAAGCGG GACGCCAAGG AGAAGCTGGA GAAGCAGCAG 
CAGATGCACA TCGTGGACAT GCTGAGCAAG GAGATCCAGG AGCTCCAGAG CAAACCGGAC 
CGCAGCGCCG AGGAGAGCGA CCGGCTGCGC AAGCTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTCCAGG AGTCGAAGCA GAAGGACGAA GATGACGAGG AGGAGGAGGA CGATGATGTG 
GACACCATGC TGATCATGCA GCGCCTGGAG GCTGAACGAA GAGCGAGGTT GCAGGACGAG 
GAGCGGAGGC GGCAGCAGCA GTTAGAAGAG ATGCGCAAGC GGGAAGCGGA AGACCGAGCG 
AGGCAAGAGG AAGAGCGCCG GCGGCAGGAG GAGGAGCGAA CAAAACGAGA CGCTGAAGAA 
AAGAGGCGAC AGGAAGAAGG GTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC 
GACGAGGCGG CGCGCAGGTT GCTGGAGCCC GAGGCGCCCG GTCTGTGCCG CCCTCCGCTT 
CCCCGGGACT ACGAGCCCCC GTCCCCGTCC CCCGCGCCCG GCGCCCCTCC TCCCCCGCCT 
CAGCGAAACG CCTCCTACCT CAAAACACAG GTCCTCTCCC CCGACTCGCT GTTCACTGCC 
AAGTTTGTTG CATACAATGA GGAGGAGGAG GAGGAGGACT GCAGCCTAGC AGGACCAAAC 
TCTTACCCAG GATCTACTGG AGCAGCTGTT GGAGCCCATG ACGCCTGTCG GGATGCAAAA 
GAGAAGCGCT CTAAAAGCCA AGATGCAGAT TCACCTGGAA GTTCTGGGGC CCCTGAAAAC 
TTGACATTCA AGGAACGCCA GCGTCTTTTT TCACAAGGTC AAGATGTATC CAATAAAGTG 
AAAGCTTCTC GTAAATTAAC AGAACTGGAG AATGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017010885.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866374.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

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CloneID	OHu96622
Clone ID Related Accession (Same CDS sequence)	XM_017010885.1
Accession Version	XM_017010885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG 
CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCGGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCA 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAGG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC CACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CGCCAACCAG 
ATAGGGCTGC CGTCTGCGCA GGTGGCTGCT GCTGAACGGA GAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATTACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGACGAT GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA 
GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG 
GAGCCCGAGG CGCCCGGTCT GTGCCGCCCT CCGCTTCCCC GGGACTACGA GCCCCCGTCC 
CCGTCCCCCG CGCCCGGCGC CCCTCCTCCC CCGCCTCAGC GAAACGCCTC CTACCTCAAA 
ACACAGGTCC TCTCCCCCGA CTCGCTGTTC ACTGCCAAGT TTGTTGCATA CAATGAGGAG 
GAGGAGGAGG AGGACTGCAG CCTAGCAGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA 
GCTGTTGGAG CCCATGACGC CTGTCGGGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866374.1
CDS	245..5671
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017010885.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010885.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG 
CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCGGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCA 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAGG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC CACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CGCCAACCAG 
ATAGGGCTGC CGTCTGCGCA GGTGGCTGCT GCTGAACGGA GAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATTACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGACGAT GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA 
GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG 
GAGCCCGAGG CGCCCGGTCT GTGCCGCCCT CCGCTTCCCC GGGACTACGA GCCCCCGTCC 
CCGTCCCCCG CGCCCGGCGC CCCTCCTCCC CCGCCTCAGC GAAACGCCTC CTACCTCAAA 
ACACAGGTCC TCTCCCCCGA CTCGCTGTTC ACTGCCAAGT TTGTTGCATA CAATGAGGAG 
GAGGAGGAGG AGGACTGCAG CCTAGCAGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA 
GCTGTTGGAG CCCATGACGC CTGTCGGGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017010887.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866376.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

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CloneID	OHu96624
Clone ID Related Accession (Same CDS sequence)	XM_017010887.1
Accession Version	XM_017010887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGGA AGGCAGATTT GTTCTTAAGA ATGAGAATGA CGCCATTCCT CCTAAGTGAA 
AATTCTCGAC TGGCTGCTGA GGTTTACAAA GACATGCCGG AAACCAGCTT TACTCGAACC 
ATTTCTAATC CTGAGGTGGT TATGAAACGA CGGAGGCAGC AAAAATTGGA AAAGAGAATG 
CAGGAATTTC GGAGCTCAGA TGGGCGGCCT GATTCAGGTG GAACATTGAG AATTTATGCA 
GATAGTTTAA AACCAAATAT TCCCTACAAG ACAATCCTGC TGTCTACTAC AGATCCTGCA 
GACTTTGCTG TGGCTGAAGC TTTAGAGAAG TATGGTCTGG AAAAAGAAAA CCCTAAGGAT 
TACTGCATCG CCCGGGTTAT GCTTCCTCCT GGAGCCCAGC ATTCTGATGA AAAGGGTGCT 
AAAGAAATTA TTCTTGATGA TGATGAGTGT CCTTTACAAA TCTTCAGGGA ATGGCCAAGT 
GACAAAGGGA TTTTAGTCTT TCAGTTGAAG AGGAGGCCAC CAGACCACAT CCCAAAGAAA 
ACCAAGAAAC ACTTGGAAGG CAAGACACCC AAGGGAAAGG AGAGAGCTGA CGGGTCTGGC 
TATGGCTCCA CCCTTCCTCC GGAGAAGCTG CCCTATTTAG TAGAGTTAAG CCCAGGGAGA 
AGGAATCACT TTGCCTACTA CAACTATCAC ACTTACGAAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT 
TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT 
GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866376.1
CDS	21..4994
Translation	MIGKADLFLRMRMTPFLLSENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017010887.1
Organism	Homo sapiens(human)
Definition	Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010887.1

ORF Insert Sequence:

ATGATCGGGA AGGCAGATTT GTTCTTAAGA ATGAGAATGA CGCCATTCCT CCTAAGTGAA 
AATTCTCGAC TGGCTGCTGA GGTTTACAAA GACATGCCGG AAACCAGCTT TACTCGAACC 
ATTTCTAATC CTGAGGTGGT TATGAAACGA CGGAGGCAGC AAAAATTGGA AAAGAGAATG 
CAGGAATTTC GGAGCTCAGA TGGGCGGCCT GATTCAGGTG GAACATTGAG AATTTATGCA 
GATAGTTTAA AACCAAATAT TCCCTACAAG ACAATCCTGC TGTCTACTAC AGATCCTGCA 
GACTTTGCTG TGGCTGAAGC TTTAGAGAAG TATGGTCTGG AAAAAGAAAA CCCTAAGGAT 
TACTGCATCG CCCGGGTTAT GCTTCCTCCT GGAGCCCAGC ATTCTGATGA AAAGGGTGCT 
AAAGAAATTA TTCTTGATGA TGATGAGTGT CCTTTACAAA TCTTCAGGGA ATGGCCAAGT 
GACAAAGGGA TTTTAGTCTT TCAGTTGAAG AGGAGGCCAC CAGACCACAT CCCAAAGAAA 
ACCAAGAAAC ACTTGGAAGG CAAGACACCC AAGGGAAAGG AGAGAGCTGA CGGGTCTGGC 
TATGGCTCCA CCCTTCCTCC GGAGAAGCTG CCCTATTTAG TAGAGTTAAG CCCAGGGAGA 
AGGAATCACT TTGCCTACTA CAACTATCAC ACTTACGAAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT 
TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT 
GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AFDN
Entrez Gene ID	4301
Full Name	afadin, adherens junction formation factor
Synonyms	AF6,MLL-AF6,MLLT4,l-afadin
General protein information	Preferred Names afadin, adherens junction formation factor Names afadin ALL1-fused gene from chromosome 6 protein myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 protein AF-6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q27
Summary	This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.[provided by RefSeq, May 2011].
Disorder MIM:	159559

Caenorhabditis elegans (roundworm)	afd-1	NP_001021660.1
Gallus gallus (chicken)	MLLT4	XP_004935593.1
Danio rerio (zebrafish)	mllt4	XP_686655.5
Homo sapiens (human)	MLLT4	NP_001193937.1
Pan troglodytes (chimpanzee)	MLLT4	XP_518857.4
	MLLT4	XP_001083153.1
Canis lupus familiaris (dog)	MLLT4	XP_005615632.1
Bos taurus (cattle)	MLLT4	XP_005211198.1
Mus musculus (house mouse)	Mllt4	NP_034936.1
Rattus norvegicus (Norway rat)	Mllt4	NP_037349.1
Xenopus tropicalis (tropical clawed frog)	mllt4	XP_002931548.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AFDN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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