afdn gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_031902298.1	XP_031758158.1	afadin isoform X7
XM_031902301.1	XP_031758161.1	afadin isoform X10
XM_031902296.1	XP_031758156.1	afadin isoform X5
XM_031902299.1	XP_031758159.1	afadin isoform X8
XM_031902300.1	XP_031758160.1	afadin isoform X9
XM_031902302.1	XP_031758162.1	afadin isoform X11
XM_012963753.2	XP_012819207.1	afadin
XM_031902293.1	XP_031758153.1	afadin isoform X2
XM_031902294.1	XP_031758154.1	afadin isoform X3
XM_031902295.1	XP_031758155.1	afadin isoform X4
XM_031902297.1	XP_031758157.1	afadin isoform X6
XM_031902292.1	XP_031758152.1	afadin isoform X1
XM_031902303.1	XP_031758163.1	afadin isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa58611	XM_031902292.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58612	XM_031902293.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58613	XM_031902294.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58614	XM_031902295.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58615	XM_031902296.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58616	XM_031902297.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58617	XM_031902298.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58618	XM_031902299.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58619	XM_031902300.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58620	XM_031902301.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58621	XM_031902302.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58622	XM_031902303.1 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa35546	XM_012963753.2 Latest version!	Xenopus tropicalis afadin, adherens junction formation factor (afdn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

afdn ( XM_031902292.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758152.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa58611
Clone ID Related Accession (Same CDS sequence)	XM_031902292.1
Accession Version	XM_031902292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGCAGACC 
GCTATGCCAG CAATCTCTAT TCTAGATTTG TTACAGGATG AAGAACGGAG ACGGCAACAA 
CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT 
CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA 
GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA 
TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT 
TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC 
CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT 
GATGATGAGG AAGACACAAA CCTATCAGGT CAGGATAAGT ACTCCAGCAT GAGAAAATCT 
TATGGTGACC TCCCTCCAGC TGCTCATAAA CCACAGCTGC CTGCTGCACG GCTGCCTCGT 
CCTGTATCCG ATGGCATCTT TCTTTCCGGC TCATTTCAGC CCCCTTCAGC CAATGCCAAC 
AGTACTGCTT CCAAAACGGG TCCACCTATA GCAAATAAAC CAAGTTTCCT CCATTACAGC 
AGCTCCAAAG GACCAAATTC TTATTCTGGA TCATCTGGTG CAGCAGTTCC TGGTAATGAA 
GTTTATAAAG ATCCGAGGGA TGTGCGACTA AAAAGCCAAG ATGGAGAAGC TTATAGCACT 
GGTGGAGCCC CAGAGAGCCT AACCTTTAAG GAGCGCCAGC GGCTCTTCTC TCAGGGCCAG 
GATGTGTCTA ACAAAGTGAA AGCTTCCAGA AAATTAATGG AACTGGAAAA TGAGTTAAAC 
ACAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758152.1
CDS	999..6707
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQEIDKGNQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARQTAMPAISILDLLQDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGQDKYSSMRKSYGDLPPAAHKPQLPAARLPRPVSDGIFLSGSFQPPSANANSTASKTGPPIANKPSFLHYSSSKGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902292.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902292.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGCAGACC 
GCTATGCCAG CAATCTCTAT TCTAGATTTG TTACAGGATG AAGAACGGAG ACGGCAACAA 
CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT 
CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA 
GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA 
TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT 
TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC 
CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT 
GATGATGAGG AAGACACAAA CCTATCAGGT CAGGATAAGT ACTCCAGCAT GAGAAAATCT 
TATGGTGACC TCCCTCCAGC TGCTCATAAA CCACAGCTGC CTGCTGCACG GCTGCCTCGT 
CCTGTATCCG ATGGCATCTT TCTTTCCGGC TCATTTCAGC CCCCTTCAGC CAATGCCAAC 
AGTACTGCTT CCAAAACGGG TCCACCTATA GCAAATAAAC CAAGTTTCCT CCATTACAGC 
AGCTCCAAAG GACCAAATTC TTATTCTGGA TCATCTGGTG CAGCAGTTCC TGGTAATGAA 
GTTTATAAAG ATCCGAGGGA TGTGCGACTA AAAAGCCAAG ATGGAGAAGC TTATAGCACT 
GGTGGAGCCC CAGAGAGCCT AACCTTTAAG GAGCGCCAGC GGCTCTTCTC TCAGGGCCAG 
GATGTGTCTA ACAAAGTGAA AGCTTCCAGA AAATTAATGG AACTGGAAAA TGAGTTAAAC 
ACAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_031902293.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758153.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X2, mRNA.

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CloneID	OXa58612
Clone ID Related Accession (Same CDS sequence)	XM_031902293.1
Accession Version	XM_031902293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT 
CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA 
AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG 
TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA 
GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG 
GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT 
ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT 
GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA 
CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT 
TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA 
TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA 
GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG 
TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG 
GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT 
TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT 
TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG 
TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA 
GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT 
ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT 
GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA 
AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT 
GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA 
GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA 
CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT 
ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC 
AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT 
GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT 
GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGGAAAT AGACAAAGGT 
AACCAGAAGC AAAAGAATAT TGCTGGAGCT CTTGCTTTCT GGATGGCAAA CGCTTCAGAG 
CTTCTCAATT TCATAAAGCA AGACCGAGAT CTCAATCGTA TCACATTGGA TGCACAAGAT 
GTTCTTGCAC ATTTAGTTCA GATGGCATTC AAATACCTGG TTCACTGTCT TCAATCTGAA 
TTGACCAGCT CTATGCCTGC TTTTATAGAT GATCCAGAAG AAAATGATTC CCAAAGGCCT 
AAAATAGAAG ACGTTTTGCA TACTCTGACA GGTGCCATGT CACTTCTAAG ACGTTGCCGG 
GTCAACGCAG CACTAACTAT TCAGCTGTTC TCGCAGCTAT TCCATTTCAT CAACATGTGG 
CTGTTCAACA GGCTTGTAAC AGATCCAGAC TCTGGTTTAT GCTCTCACTA TTGGGGGGCA 
ATAATTCGGC AGCAGCTTGC ACACATTGAG GCATGGGCTG AAAAACAAGG CCTCGAATTA 
GCTGCAGATT GTCATCTTAG CAGAATAGTT CAGGCTACTA CTCTTCTCAC TATGGACAAG 
TACTCGCATC AGGATATTCC AAATATTAAT AATACATGTT TTAAGCTGAA TTCCTTACAA 
CTGCAAGCCC TATTGCAAAA TTACCACTGT GCTCCTGATG AACCGTACAT CCCAACGGAG 
TTGATAGACA GTGTGGTGTC TGTAGCTGAA AACACTGCAG ATGAATTGGC TCGAAGTGAT 
GGCAGAGAAG TTCAGCTTGA GGAGGATCCT GATCTGCAAC TCCCTTTCCT TTTGCCTGAA 
GATGGTTATT CATGTGATGT GGTTAGAAAT ATTCCTAATG GCCTTCAAGA GTTTTTAGAA 
CCGCTCTGCC AGAGAGGTTT TTGCAGACTT GTCCCTCACT CACGGTCACC AGGCATGTGG 
ACAATTTACT TTGAAGGAGC TGAATTTGAA AATAATTTGT TGACCGACAA TACAGACCTG 
CCATTAAGAA AAGAACCTGA AATAATCACT GTGACACTTA AAAAGCAGAA TGGAATGGGT 
TTGAGTATTG TTGCAGCAAA GGGTGCTGGA CAAGAAAAAC TTGGGATTTA CATTAAGTCT 
GTGGTGAAAG GCGGTGCTGC AGAAGTGGAT GGTCGTTTAG CTGCAGGGGA CCAACTTATA 
AGTGTGGATG GGCAAAGTTT AGTTGGTTTG TCCCAAGAAA GAGCTGCAGA GCTTATGACT 
AGAACTGGGG CCGTAGTAAC CCTTGAGGTC GCAAAGCAAG GCGCAATATA CCATGGACTT 
GCCACACTAC TAAACCAACC ATCTCCTCTC ATGCAAAGAG ATCGACGTGG CTCAGGAAAA 
CCACGACCAA AGAGTGAAGG TTTTGAGCTT TACAATAATG CTTCCCAAAA TGGGTCTCCT 
GATAGCCCCC AGACACAATG GCCAGAATAC ACCGAACCCA AAAAGTTACC AGGCGATGAT 
AAACTAATGA AAAACCGTGC AGATCATCGA TCCAGTCCTA ATGTGGCCAA TCAACCCCAG 
AGTCCAGGTG GAAAAACTAC TTATTCAAGC AGCTCAACAG TGAAGATAAC ATCTGTCTCC 
ACTGGAAACC TATGCACTGA GGAACAGACT GCACCAGCAA GACCAGAAGC TTATCCTATC 
CCTACACAAA CATATGCAAG AGAGTATTTT ACTTTTCCAA CTCCAAAATC TCAAGATAGA 
ATAGGACCAG CTCAGAACCA ATGGCAAGGC TATGAGGATA ATTTCCAAGT TCAGATGGGT 
AACAACCATG CCAGAAATAC TTCGGCTCAA CAAGTTGCTT ATTCTGAGGA AGAGCTAAGG 
GATAATTTAA CATTCCAGTC AGAAAGAGGG CGCGTTGAAC CCCAAGTGCG GAAAGATGTG 
GAACATCTGG ATGGTAATGA TACTTGGTTC AATCGAGGCT CGTCTATGGA ATCAAGTGCA 
TCTTGTCAAG ACCTTAATAA TTCAAAGACA CATTCTGTAG GTTCTTTGAG CAAAACAGGA 
CCTGGTCGCT GGAAAACACC AATTTCTTCT CAGTCAGCTA CTGTTCTTTT GCCGCAGCCT 
GTTAGAACAG ACCTTCCTCC TCCACCACCT CCACCAGTTC ATTATGGAGC AGAATTTGAG 
GGAGTCCCAA TAGATTTACC TCTACCACCC CCACCATCTG AGAGCCAACT AGGGCTTTCA 
ACAAACTCAC AGGCAGCTGC ACTAGAAAGA AAGAAGCGGG AAGAACATCA GAGGTGGTAT 
GAGAAAGAGA AGGCACGTTT GGAAGAAGAA AGAGAAAGGA AAAGGCGAGA GCAGGAAAGA 
AAGCTAGGCC AGGTTCGTCC TCAGCCACCA ACTATACCTC CACAACCAGT AAATCCACAA 
TCTCCTCCGT TGATTCCTCC TCCAAATGTT GCACAAGCCA GAACAGAGAA ACCTGCCACT 
CTTCCTCGGC AACAAGAAAC TGTTATCAGG GAGCTACAAC CTCAGCAGCA GCCACGAACC 
ATAGAAAGAA GAGACCTCCA GTACATTACA GTCAGTAAAG AGGAGCTTTC TTCCAGTGAC 
AGCCTTTCCC CAGATCCCTG GAAAAGGGAT GCCAAGGAGA AGCTAGAAAA ACAGCAGCAG 
ATGCATATAG TGGATATGCT GAGCAAAGAG ATCAATGAAC TGCAAAACAA GCCTGACCGA 
ACTTCTGAAG AAAATGACCG ACTCCGCAAA TTAATGCTTG AATGGCAGTT TCAGAAGAGA 
CTACAAGAGT CCCAGCAGAA GGATGACGAT GACGACGATG ACGATGATGA TGATGATGAT 
GTGGATACCA TGCTCATCAT GCAGCGTCTG GAAGCTGAAA GGAAAGCAAG GCAGACCGCT 
ATGCCAGCAA TCTCTATTCT AGATTTGTTA CAGGATGAAG AACGGAGACG GCAACAACAT 
TTGGAAGAGG CGCGCAAACG GGAGGCAGAA GATCGCCTTA GGCAGGAAGA AGAGCATCGT 
CATCAAGAGG AGGAGCGGGC AAGGAGAGAG GCTGAAGAAA AGAGGAGACA GGAAGAAGAG 
TACTACAACC GCTTGGAAGC TGAGAGGCGC AGAATTCATG AAGAAGCAGA GCGGCGATTG 
TTAGACCCTG AAGAGCCTGG TCTGTACAGA CCTCCACTCC CACGGGAATA TGAGCTTTCC 
TCCCCAACCC TCCCACCTAA TCCTCCGCCT CCCCCTCCTC AGCGTAACAC CTCTTACCTC 
AAAACACAAG CCCTCTCCCC TGACACAGTT TACACTGCCA AGTTTGTTAC ATACACTGAT 
GATGAGGAAG ACACAAACCT ATCAGGTCAG GATAAGTACT CCAGCATGAG AAAATCTTAT 
GGTGACCTCC CTCCAGCTGC TCATAAACCA CAGCTGCCTG CTGCACGGCT GCCTCGTCCT 
GTATCCGATG GCATCTTTCT TTCCGGCTCA TTTCAGCCCC CTTCAGCCAA TGCCAACAGT 
ACTGCTTCCA AAACGGGTCC ACCTATAGCA AATAAACCAA GTTTCCTCCA TTACAGCAGC 
TCCAAAGGAC CAAATTCTTA TTCTGGATCA TCTGGTGCAG CAGTTCCTGG TAATGAAGTT 
TATAAAGATC CGAGGGATGT GCGACTAAAA AGCCAAGATG GAGAAGCTTA TAGCACTGGT 
GGAGCCCCAG AGAGCCTAAC CTTTAAGGAG CGCCAGCGGC TCTTCTCTCA GGGCCAGGAT 
GTGTCTAACA AAGTGAAAGC TTCCAGAAAA TTAATGGAAC TGGAAAATGA GTTAAACACA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758153.1
CDS	1000..6705
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQEIDKGNQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARQTAMPAISILDLLQDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGQDKYSSMRKSYGDLPPAAHKPQLPAARLPRPVSDGIFLSGSFQPPSANANSTASKTGPPIANKPSFLHYSSSKGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902293.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902293.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT 
CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA 
AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG 
TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA 
GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG 
GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT 
ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT 
GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA 
CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT 
TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA 
TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA 
GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG 
TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG 
GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT 
TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT 
TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG 
TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA 
GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT 
ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT 
GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA 
AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT 
GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA 
GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA 
CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT 
ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC 
AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT 
GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT 
GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGGAAAT AGACAAAGGT 
AACCAGAAGC AAAAGAATAT TGCTGGAGCT CTTGCTTTCT GGATGGCAAA CGCTTCAGAG 
CTTCTCAATT TCATAAAGCA AGACCGAGAT CTCAATCGTA TCACATTGGA TGCACAAGAT 
GTTCTTGCAC ATTTAGTTCA GATGGCATTC AAATACCTGG TTCACTGTCT TCAATCTGAA 
TTGACCAGCT CTATGCCTGC TTTTATAGAT GATCCAGAAG AAAATGATTC CCAAAGGCCT 
AAAATAGAAG ACGTTTTGCA TACTCTGACA GGTGCCATGT CACTTCTAAG ACGTTGCCGG 
GTCAACGCAG CACTAACTAT TCAGCTGTTC TCGCAGCTAT TCCATTTCAT CAACATGTGG 
CTGTTCAACA GGCTTGTAAC AGATCCAGAC TCTGGTTTAT GCTCTCACTA TTGGGGGGCA 
ATAATTCGGC AGCAGCTTGC ACACATTGAG GCATGGGCTG AAAAACAAGG CCTCGAATTA 
GCTGCAGATT GTCATCTTAG CAGAATAGTT CAGGCTACTA CTCTTCTCAC TATGGACAAG 
TACTCGCATC AGGATATTCC AAATATTAAT AATACATGTT TTAAGCTGAA TTCCTTACAA 
CTGCAAGCCC TATTGCAAAA TTACCACTGT GCTCCTGATG AACCGTACAT CCCAACGGAG 
TTGATAGACA GTGTGGTGTC TGTAGCTGAA AACACTGCAG ATGAATTGGC TCGAAGTGAT 
GGCAGAGAAG TTCAGCTTGA GGAGGATCCT GATCTGCAAC TCCCTTTCCT TTTGCCTGAA 
GATGGTTATT CATGTGATGT GGTTAGAAAT ATTCCTAATG GCCTTCAAGA GTTTTTAGAA 
CCGCTCTGCC AGAGAGGTTT TTGCAGACTT GTCCCTCACT CACGGTCACC AGGCATGTGG 
ACAATTTACT TTGAAGGAGC TGAATTTGAA AATAATTTGT TGACCGACAA TACAGACCTG 
CCATTAAGAA AAGAACCTGA AATAATCACT GTGACACTTA AAAAGCAGAA TGGAATGGGT 
TTGAGTATTG TTGCAGCAAA GGGTGCTGGA CAAGAAAAAC TTGGGATTTA CATTAAGTCT 
GTGGTGAAAG GCGGTGCTGC AGAAGTGGAT GGTCGTTTAG CTGCAGGGGA CCAACTTATA 
AGTGTGGATG GGCAAAGTTT AGTTGGTTTG TCCCAAGAAA GAGCTGCAGA GCTTATGACT 
AGAACTGGGG CCGTAGTAAC CCTTGAGGTC GCAAAGCAAG GCGCAATATA CCATGGACTT 
GCCACACTAC TAAACCAACC ATCTCCTCTC ATGCAAAGAG ATCGACGTGG CTCAGGAAAA 
CCACGACCAA AGAGTGAAGG TTTTGAGCTT TACAATAATG CTTCCCAAAA TGGGTCTCCT 
GATAGCCCCC AGACACAATG GCCAGAATAC ACCGAACCCA AAAAGTTACC AGGCGATGAT 
AAACTAATGA AAAACCGTGC AGATCATCGA TCCAGTCCTA ATGTGGCCAA TCAACCCCAG 
AGTCCAGGTG GAAAAACTAC TTATTCAAGC AGCTCAACAG TGAAGATAAC ATCTGTCTCC 
ACTGGAAACC TATGCACTGA GGAACAGACT GCACCAGCAA GACCAGAAGC TTATCCTATC 
CCTACACAAA CATATGCAAG AGAGTATTTT ACTTTTCCAA CTCCAAAATC TCAAGATAGA 
ATAGGACCAG CTCAGAACCA ATGGCAAGGC TATGAGGATA ATTTCCAAGT TCAGATGGGT 
AACAACCATG CCAGAAATAC TTCGGCTCAA CAAGTTGCTT ATTCTGAGGA AGAGCTAAGG 
GATAATTTAA CATTCCAGTC AGAAAGAGGG CGCGTTGAAC CCCAAGTGCG GAAAGATGTG 
GAACATCTGG ATGGTAATGA TACTTGGTTC AATCGAGGCT CGTCTATGGA ATCAAGTGCA 
TCTTGTCAAG ACCTTAATAA TTCAAAGACA CATTCTGTAG GTTCTTTGAG CAAAACAGGA 
CCTGGTCGCT GGAAAACACC AATTTCTTCT CAGTCAGCTA CTGTTCTTTT GCCGCAGCCT 
GTTAGAACAG ACCTTCCTCC TCCACCACCT CCACCAGTTC ATTATGGAGC AGAATTTGAG 
GGAGTCCCAA TAGATTTACC TCTACCACCC CCACCATCTG AGAGCCAACT AGGGCTTTCA 
ACAAACTCAC AGGCAGCTGC ACTAGAAAGA AAGAAGCGGG AAGAACATCA GAGGTGGTAT 
GAGAAAGAGA AGGCACGTTT GGAAGAAGAA AGAGAAAGGA AAAGGCGAGA GCAGGAAAGA 
AAGCTAGGCC AGGTTCGTCC TCAGCCACCA ACTATACCTC CACAACCAGT AAATCCACAA 
TCTCCTCCGT TGATTCCTCC TCCAAATGTT GCACAAGCCA GAACAGAGAA ACCTGCCACT 
CTTCCTCGGC AACAAGAAAC TGTTATCAGG GAGCTACAAC CTCAGCAGCA GCCACGAACC 
ATAGAAAGAA GAGACCTCCA GTACATTACA GTCAGTAAAG AGGAGCTTTC TTCCAGTGAC 
AGCCTTTCCC CAGATCCCTG GAAAAGGGAT GCCAAGGAGA AGCTAGAAAA ACAGCAGCAG 
ATGCATATAG TGGATATGCT GAGCAAAGAG ATCAATGAAC TGCAAAACAA GCCTGACCGA 
ACTTCTGAAG AAAATGACCG ACTCCGCAAA TTAATGCTTG AATGGCAGTT TCAGAAGAGA 
CTACAAGAGT CCCAGCAGAA GGATGACGAT GACGACGATG ACGATGATGA TGATGATGAT 
GTGGATACCA TGCTCATCAT GCAGCGTCTG GAAGCTGAAA GGAAAGCAAG GCAGACCGCT 
ATGCCAGCAA TCTCTATTCT AGATTTGTTA CAGGATGAAG AACGGAGACG GCAACAACAT 
TTGGAAGAGG CGCGCAAACG GGAGGCAGAA GATCGCCTTA GGCAGGAAGA AGAGCATCGT 
CATCAAGAGG AGGAGCGGGC AAGGAGAGAG GCTGAAGAAA AGAGGAGACA GGAAGAAGAG 
TACTACAACC GCTTGGAAGC TGAGAGGCGC AGAATTCATG AAGAAGCAGA GCGGCGATTG 
TTAGACCCTG AAGAGCCTGG TCTGTACAGA CCTCCACTCC CACGGGAATA TGAGCTTTCC 
TCCCCAACCC TCCCACCTAA TCCTCCGCCT CCCCCTCCTC AGCGTAACAC CTCTTACCTC 
AAAACACAAG CCCTCTCCCC TGACACAGTT TACACTGCCA AGTTTGTTAC ATACACTGAT 
GATGAGGAAG ACACAAACCT ATCAGGTCAG GATAAGTACT CCAGCATGAG AAAATCTTAT 
GGTGACCTCC CTCCAGCTGC TCATAAACCA CAGCTGCCTG CTGCACGGCT GCCTCGTCCT 
GTATCCGATG GCATCTTTCT TTCCGGCTCA TTTCAGCCCC CTTCAGCCAA TGCCAACAGT 
ACTGCTTCCA AAACGGGTCC ACCTATAGCA AATAAACCAA GTTTCCTCCA TTACAGCAGC 
TCCAAAGGAC CAAATTCTTA TTCTGGATCA TCTGGTGCAG CAGTTCCTGG TAATGAAGTT 
TATAAAGATC CGAGGGATGT GCGACTAAAA AGCCAAGATG GAGAAGCTTA TAGCACTGGT 
GGAGCCCCAG AGAGCCTAAC CTTTAAGGAG CGCCAGCGGC TCTTCTCTCA GGGCCAGGAT 
GTGTCTAACA AAGTGAAAGC TTCCAGAAAA TTAATGGAAC TGGAAAATGA GTTAAACACA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_031902294.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758154.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa58613
Clone ID Related Accession (Same CDS sequence)	XM_031902294.1
Accession Version	XM_031902294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT 
ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG 
CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT 
CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT 
GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG 
CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT 
ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA 
ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT 
GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT 
AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT 
CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA 
AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG 
TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT 
GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT 
GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT 
TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA 
GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT 
GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA 
AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT 
GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT 
TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA 
ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA 
CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA 
GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA 
TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT 
GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT 
ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT 
GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA 
AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC 
CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT 
ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG 
TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT 
GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT 
AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA 
CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT 
CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA 
CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT 
GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT 
TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT 
CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT 
CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA 
ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC 
CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC 
TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG 
CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC 
CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG 
AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC 
ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGCAGACCG CTATGCCAGC AATCTCTATT 
CTAGATTTGT TACAGGATGA AGAACGGAGA CGGCAACAAC ATTTGGAAGA GGCGCGCAAA 
CGGGAGGCAG AAGATCGCCT TAGGCAGGAA GAAGAGCATC GTCATCAAGA GGAGGAGCGG 
GCAAGGAGAG AGGCTGAAGA AAAGAGGAGA CAGGAAGAAG AGTACTACAA CCGCTTGGAA 
GCTGAGAGGC GCAGAATTCA TGAAGAAGCA GAGCGGCGAT TGTTAGACCC TGAAGAGCCT 
GGTCTGTACA GACCTCCACT CCCACGGGAA TATGAGCTTT CCTCCCCAAC CCTCCCACCT 
AATCCTCCGC CTCCCCCTCC TCAGCGTAAC ACCTCTTACC TCAAAACACA AGCCCTCTCC 
CCTGACACAG TTTACACTGC CAAGTTTGTT ACATACACTG ATGATGAGGA AGACACAAAC 
CTATCAGGTC AGGATAAGTA CTCCAGCATG AGAAAATCTT ATGGTGACCT CCCTCCAGCT 
GCTCATAAAC CACAGCTGCC TGCTGCACGG CTGCCTCGTC CTGTATCCGA TGGCATCTTT 
CTTTCCGGCT CATTTCAGCC CCCTTCAGCC AATGCCAACA GTACTGCTTC CAAAACGGGT 
CCACCTATAG CAAATAAACC AAGTTTCCTC CATTACAGCA GCTCCAAAGG ACCAAATTCT 
TATTCTGGAT CATCTGGTGC AGCAGTTCCT GGTAATGAAG TTTATAAAGA TCCGAGGGAT 
GTGCGACTAA AAAGCCAAGA TGGAGAAGCT TATAGCACTG GTGGAGCCCC AGAGAGCCTA 
ACCTTTAAGG AGCGCCAGCG GCTCTTCTCT CAGGGCCAGG ATGTGTCTAA CAAAGTGAAA 
GCTTCCAGAA AATTAATGGA ACTGGAAAAT GAGTTAAACA CAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758154.1
CDS	998..6685
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARQTAMPAISILDLLQDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGQDKYSSMRKSYGDLPPAAHKPQLPAARLPRPVSDGIFLSGSFQPPSANANSTASKTGPPIANKPSFLHYSSSKGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902294.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902294.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT 
ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG 
CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT 
CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT 
GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG 
CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT 
ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA 
ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT 
GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT 
AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT 
CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA 
AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG 
TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT 
GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT 
GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT 
TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA 
GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT 
GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA 
AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT 
GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT 
TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA 
ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA 
CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA 
GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA 
TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT 
GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT 
ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT 
GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA 
AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC 
CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT 
ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG 
TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT 
GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT 
AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA 
CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT 
CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA 
CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT 
GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT 
TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT 
CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT 
CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA 
ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC 
CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC 
TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG 
CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC 
CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG 
AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC 
ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGCAGACCG CTATGCCAGC AATCTCTATT 
CTAGATTTGT TACAGGATGA AGAACGGAGA CGGCAACAAC ATTTGGAAGA GGCGCGCAAA 
CGGGAGGCAG AAGATCGCCT TAGGCAGGAA GAAGAGCATC GTCATCAAGA GGAGGAGCGG 
GCAAGGAGAG AGGCTGAAGA AAAGAGGAGA CAGGAAGAAG AGTACTACAA CCGCTTGGAA 
GCTGAGAGGC GCAGAATTCA TGAAGAAGCA GAGCGGCGAT TGTTAGACCC TGAAGAGCCT 
GGTCTGTACA GACCTCCACT CCCACGGGAA TATGAGCTTT CCTCCCCAAC CCTCCCACCT 
AATCCTCCGC CTCCCCCTCC TCAGCGTAAC ACCTCTTACC TCAAAACACA AGCCCTCTCC 
CCTGACACAG TTTACACTGC CAAGTTTGTT ACATACACTG ATGATGAGGA AGACACAAAC 
CTATCAGGTC AGGATAAGTA CTCCAGCATG AGAAAATCTT ATGGTGACCT CCCTCCAGCT 
GCTCATAAAC CACAGCTGCC TGCTGCACGG CTGCCTCGTC CTGTATCCGA TGGCATCTTT 
CTTTCCGGCT CATTTCAGCC CCCTTCAGCC AATGCCAACA GTACTGCTTC CAAAACGGGT 
CCACCTATAG CAAATAAACC AAGTTTCCTC CATTACAGCA GCTCCAAAGG ACCAAATTCT 
TATTCTGGAT CATCTGGTGC AGCAGTTCCT GGTAATGAAG TTTATAAAGA TCCGAGGGAT 
GTGCGACTAA AAAGCCAAGA TGGAGAAGCT TATAGCACTG GTGGAGCCCC AGAGAGCCTA 
ACCTTTAAGG AGCGCCAGCG GCTCTTCTCT CAGGGCCAGG ATGTGTCTAA CAAAGTGAAA 
GCTTCCAGAA AATTAATGGA ACTGGAAAAT GAGTTAAACA CAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_031902295.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758155.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa58614
Clone ID Related Accession (Same CDS sequence)	XM_031902295.1
Accession Version	XM_031902295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGTTACAG 
GATGAAGAAC GGAGACGGCA ACAACATTTG GAAGAGGCGC GCAAACGGGA GGCAGAAGAT 
CGCCTTAGGC AGGAAGAAGA GCATCGTCAT CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT 
GAAGAAAAGA GGAGACAGGA AGAAGAGTAC TACAACCGCT TGGAAGCTGA GAGGCGCAGA 
ATTCATGAAG AAGCAGAGCG GCGATTGTTA GACCCTGAAG AGCCTGGTCT GTACAGACCT 
CCACTCCCAC GGGAATATGA GCTTTCCTCC CCAACCCTCC CACCTAATCC TCCGCCTCCC 
CCTCCTCAGC GTAACACCTC TTACCTCAAA ACACAAGCCC TCTCCCCTGA CACAGTTTAC 
ACTGCCAAGT TTGTTACATA CACTGATGAT GAGGAAGACA CAAACCTATC AGGTCAGGAT 
AAGTACTCCA GCATGAGAAA ATCTTATGGT GACCTCCCTC CAGCTGCTCA TAAACCACAG 
CTGCCTGCTG CACGGCTGCC TCGTCCTGTA TCCGATGGCA TCTTTCTTTC CGGCTCATTT 
CAGCCCCCTT CAGCCAATGC CAACAGTACT GCTTCCAAAA CGGGTCCACC TATAGCAAAT 
AAACCAAGTT TCCTCCATTA CAGCAGCTCC AAAGGACCAA ATTCTTATTC TGGATCATCT 
GGTGCAGCAG TTCCTGGTAA TGAAGTTTAT AAAGATCCGA GGGATGTGCG ACTAAAAAGC 
CAAGATGGAG AAGCTTATAG CACTGGTGGA GCCCCAGAGA GCCTAACCTT TAAGGAGCGC 
CAGCGGCTCT TCTCTCAGGG CCAGGATGTG TCTAACAAAG TGAAAGCTTC CAGAAAATTA 
ATGGAACTGG AAAATGAGTT AAACACAAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758155.1
CDS	997..6669
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQEIDKGNQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARLQDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGQDKYSSMRKSYGDLPPAAHKPQLPAARLPRPVSDGIFLSGSFQPPSANANSTASKTGPPIANKPSFLHYSSSKGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902295.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902295.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGTTACAG 
GATGAAGAAC GGAGACGGCA ACAACATTTG GAAGAGGCGC GCAAACGGGA GGCAGAAGAT 
CGCCTTAGGC AGGAAGAAGA GCATCGTCAT CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT 
GAAGAAAAGA GGAGACAGGA AGAAGAGTAC TACAACCGCT TGGAAGCTGA GAGGCGCAGA 
ATTCATGAAG AAGCAGAGCG GCGATTGTTA GACCCTGAAG AGCCTGGTCT GTACAGACCT 
CCACTCCCAC GGGAATATGA GCTTTCCTCC CCAACCCTCC CACCTAATCC TCCGCCTCCC 
CCTCCTCAGC GTAACACCTC TTACCTCAAA ACACAAGCCC TCTCCCCTGA CACAGTTTAC 
ACTGCCAAGT TTGTTACATA CACTGATGAT GAGGAAGACA CAAACCTATC AGGTCAGGAT 
AAGTACTCCA GCATGAGAAA ATCTTATGGT GACCTCCCTC CAGCTGCTCA TAAACCACAG 
CTGCCTGCTG CACGGCTGCC TCGTCCTGTA TCCGATGGCA TCTTTCTTTC CGGCTCATTT 
CAGCCCCCTT CAGCCAATGC CAACAGTACT GCTTCCAAAA CGGGTCCACC TATAGCAAAT 
AAACCAAGTT TCCTCCATTA CAGCAGCTCC AAAGGACCAA ATTCTTATTC TGGATCATCT 
GGTGCAGCAG TTCCTGGTAA TGAAGTTTAT AAAGATCCGA GGGATGTGCG ACTAAAAAGC 
CAAGATGGAG AAGCTTATAG CACTGGTGGA GCCCCAGAGA GCCTAACCTT TAAGGAGCGC 
CAGCGGCTCT TCTCTCAGGG CCAGGATGTG TCTAACAAAG TGAAAGCTTC CAGAAAATTA 
ATGGAACTGG AAAATGAGTT AAACACAAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_031902296.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758156.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X5, mRNA.

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CloneID	OXa58615
Clone ID Related Accession (Same CDS sequence)	XM_031902296.1
Accession Version	XM_031902296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGGATGAA 
GAACGGAGAC GGCAACAACA TTTGGAAGAG GCGCGCAAAC GGGAGGCAGA AGATCGCCTT 
AGGCAGGAAG AAGAGCATCG TCATCAAGAG GAGGAGCGGG CAAGGAGAGA GGCTGAAGAA 
AAGAGGAGAC AGGAAGAAGA GTACTACAAC CGCTTGGAAG CTGAGAGGCG CAGAATTCAT 
GAAGAAGCAG AGCGGCGATT GTTAGACCCT GAAGAGCCTG GTCTGTACAG ACCTCCACTC 
CCACGGGAAT ATGAGCTTTC CTCCCCAACC CTCCCACCTA ATCCTCCGCC TCCCCCTCCT 
CAGCGTAACA CCTCTTACCT CAAAACACAA GCCCTCTCCC CTGACACAGT TTACACTGCC 
AAGTTTGTTA CATACACTGA TGATGAGGAA GACACAAACC TATCAGGTCA GGATAAGTAC 
TCCAGCATGA GAAAATCTTA TGGTGACCTC CCTCCAGCTG CTCATAAACC ACAGCTGCCT 
GCTGCACGGC TGCCTCGTCC TGTATCCGAT GGCATCTTTC TTTCCGGCTC ATTTCAGCCC 
CCTTCAGCCA ATGCCAACAG TACTGCTTCC AAAACGGGTC CACCTATAGC AAATAAACCA 
AGTTTCCTCC ATTACAGCAG CTCCAAAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA 
GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT 
GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG 
CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA 
CTGGAAAATG AGTTAAACAC AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758156.1
CDS	998..6664
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQEIDKGNQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGQDKYSSMRKSYGDLPPAAHKPQLPAARLPRPVSDGIFLSGSFQPPSANANSTASKTGPPIANKPSFLHYSSSKGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902296.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902296.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGGATGAA 
GAACGGAGAC GGCAACAACA TTTGGAAGAG GCGCGCAAAC GGGAGGCAGA AGATCGCCTT 
AGGCAGGAAG AAGAGCATCG TCATCAAGAG GAGGAGCGGG CAAGGAGAGA GGCTGAAGAA 
AAGAGGAGAC AGGAAGAAGA GTACTACAAC CGCTTGGAAG CTGAGAGGCG CAGAATTCAT 
GAAGAAGCAG AGCGGCGATT GTTAGACCCT GAAGAGCCTG GTCTGTACAG ACCTCCACTC 
CCACGGGAAT ATGAGCTTTC CTCCCCAACC CTCCCACCTA ATCCTCCGCC TCCCCCTCCT 
CAGCGTAACA CCTCTTACCT CAAAACACAA GCCCTCTCCC CTGACACAGT TTACACTGCC 
AAGTTTGTTA CATACACTGA TGATGAGGAA GACACAAACC TATCAGGTCA GGATAAGTAC 
TCCAGCATGA GAAAATCTTA TGGTGACCTC CCTCCAGCTG CTCATAAACC ACAGCTGCCT 
GCTGCACGGC TGCCTCGTCC TGTATCCGAT GGCATCTTTC TTTCCGGCTC ATTTCAGCCC 
CCTTCAGCCA ATGCCAACAG TACTGCTTCC AAAACGGGTC CACCTATAGC AAATAAACCA 
AGTTTCCTCC ATTACAGCAG CTCCAAAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA 
GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT 
GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG 
CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA 
CTGGAAAATG AGTTAAACAC AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_031902297.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758157.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X6, mRNA.

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CloneID	OXa58616
Clone ID Related Accession (Same CDS sequence)	XM_031902297.1
Accession Version	XM_031902297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGCAGACC 
GCTATGCCAG CAATCTCTAT TCTAGATTTG TTACAGGATG AAGAACGGAG ACGGCAACAA 
CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT 
CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA 
GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA 
TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT 
TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC 
CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT 
GATGATGAGG AAGACACAAA CCTATCAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA 
GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT 
GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG 
CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA 
CTGGAAAATG AGTTAAACAC AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758157.1
CDS	1000..6486
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQEIDKGNQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARQTAMPAISILDLLQDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902297.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902297.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGCAGACC 
GCTATGCCAG CAATCTCTAT TCTAGATTTG TTACAGGATG AAGAACGGAG ACGGCAACAA 
CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT 
CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA 
GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA 
TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT 
TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC 
CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT 
GATGATGAGG AAGACACAAA CCTATCAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA 
GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT 
GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG 
CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA 
CTGGAAAATG AGTTAAACAC AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_031902298.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758158.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X7, mRNA.

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CloneID	OXa58617
Clone ID Related Accession (Same CDS sequence)	XM_031902298.1
Accession Version	XM_031902298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGTTACAG 
GATGAAGAAC GGAGACGGCA ACAACATTTG GAAGAGGCGC GCAAACGGGA GGCAGAAGAT 
CGCCTTAGGC AGGAAGAAGA GCATCGTCAT CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT 
GAAGAAAAGA GGAGACAGGA AGAAGAGTAC TACAACCGCT TGGAAGCTGA GAGGCGCAGA 
ATTCATGAAG AAGCAGAGCG GCGATTGTTA GACCCTGAAG AGCCTGGTCT GTACAGACCT 
CCACTCCCAC GGGAATATGA GCTTTCCTCC CCAACCCTCC CACCTAATCC TCCGCCTCCC 
CCTCCTCAGC GTAACACCTC TTACCTCAAA ACACAAGCCC TCTCCCCTGA CACAGTTTAC 
ACTGCCAAGT TTGTTACATA CACTGATGAT GAGGAAGACA CAAACCTATC AGGACCAAAT 
TCTTATTCTG GATCATCTGG TGCAGCAGTT CCTGGTAATG AAGTTTATAA AGATCCGAGG 
GATGTGCGAC TAAAAAGCCA AGATGGAGAA GCTTATAGCA CTGGTGGAGC CCCAGAGAGC 
CTAACCTTTA AGGAGCGCCA GCGGCTCTTC TCTCAGGGCC AGGATGTGTC TAACAAAGTG 
AAAGCTTCCA GAAAATTAAT GGAACTGGAA AATGAGTTAA ACACAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758158.1
CDS	998..6448
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQEIDKGNQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARLQDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902298.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902298.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGTTACAG 
GATGAAGAAC GGAGACGGCA ACAACATTTG GAAGAGGCGC GCAAACGGGA GGCAGAAGAT 
CGCCTTAGGC AGGAAGAAGA GCATCGTCAT CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT 
GAAGAAAAGA GGAGACAGGA AGAAGAGTAC TACAACCGCT TGGAAGCTGA GAGGCGCAGA 
ATTCATGAAG AAGCAGAGCG GCGATTGTTA GACCCTGAAG AGCCTGGTCT GTACAGACCT 
CCACTCCCAC GGGAATATGA GCTTTCCTCC CCAACCCTCC CACCTAATCC TCCGCCTCCC 
CCTCCTCAGC GTAACACCTC TTACCTCAAA ACACAAGCCC TCTCCCCTGA CACAGTTTAC 
ACTGCCAAGT TTGTTACATA CACTGATGAT GAGGAAGACA CAAACCTATC AGGACCAAAT 
TCTTATTCTG GATCATCTGG TGCAGCAGTT CCTGGTAATG AAGTTTATAA AGATCCGAGG 
GATGTGCGAC TAAAAAGCCA AGATGGAGAA GCTTATAGCA CTGGTGGAGC CCCAGAGAGC 
CTAACCTTTA AGGAGCGCCA GCGGCTCTTC TCTCAGGGCC AGGATGTGTC TAACAAAGTG 
AAAGCTTCCA GAAAATTAAT GGAACTGGAA AATGAGTTAA ACACAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_031902299.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758159.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X8, mRNA.

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CloneID	OXa58618
Clone ID Related Accession (Same CDS sequence)	XM_031902299.1
Accession Version	XM_031902299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGGATGAA 
GAACGGAGAC GGCAACAACA TTTGGAAGAG GCGCGCAAAC GGGAGGCAGA AGATCGCCTT 
AGGCAGGAAG AAGAGCATCG TCATCAAGAG GAGGAGCGGG CAAGGAGAGA GGCTGAAGAA 
AAGAGGAGAC AGGAAGAAGA GTACTACAAC CGCTTGGAAG CTGAGAGGCG CAGAATTCAT 
GAAGAAGCAG AGCGGCGATT GTTAGACCCT GAAGAGCCTG GTCTGTACAG ACCTCCACTC 
CCACGGGAAT ATGAGCTTTC CTCCCCAACC CTCCCACCTA ATCCTCCGCC TCCCCCTCCT 
CAGCGTAACA CCTCTTACCT CAAAACACAA GCCCTCTCCC CTGACACAGT TTACACTGCC 
AAGTTTGTTA CATACACTGA TGATGAGGAA GACACAAACC TATCAGGACC AAATTCTTAT 
TCTGGATCAT CTGGTGCAGC AGTTCCTGGT AATGAAGTTT ATAAAGATCC GAGGGATGTG 
CGACTAAAAA GCCAAGATGG AGAAGCTTAT AGCACTGGTG GAGCCCCAGA GAGCCTAACC 
TTTAAGGAGC GCCAGCGGCT CTTCTCTCAG GGCCAGGATG TGTCTAACAA AGTGAAAGCT 
TCCAGAAAAT TAATGGAACT GGAAAATGAG TTAAACACAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758159.1
CDS	999..6443
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQEIDKGNQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902299.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902299.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGGA AATAGACAAA 
GGTAACCAGA AGCAAAAGAA TATTGCTGGA GCTCTTGCTT TCTGGATGGC AAACGCTTCA 
GAGCTTCTCA ATTTCATAAA GCAAGACCGA GATCTCAATC GTATCACATT GGATGCACAA 
GATGTTCTTG CACATTTAGT TCAGATGGCA TTCAAATACC TGGTTCACTG TCTTCAATCT 
GAATTGACCA GCTCTATGCC TGCTTTTATA GATGATCCAG AAGAAAATGA TTCCCAAAGG 
CCTAAAATAG AAGACGTTTT GCATACTCTG ACAGGTGCCA TGTCACTTCT AAGACGTTGC 
CGGGTCAACG CAGCACTAAC TATTCAGCTG TTCTCGCAGC TATTCCATTT CATCAACATG 
TGGCTGTTCA ACAGGCTTGT AACAGATCCA GACTCTGGTT TATGCTCTCA CTATTGGGGG 
GCAATAATTC GGCAGCAGCT TGCACACATT GAGGCATGGG CTGAAAAACA AGGCCTCGAA 
TTAGCTGCAG ATTGTCATCT TAGCAGAATA GTTCAGGCTA CTACTCTTCT CACTATGGAC 
AAGTACTCGC ATCAGGATAT TCCAAATATT AATAATACAT GTTTTAAGCT GAATTCCTTA 
CAACTGCAAG CCCTATTGCA AAATTACCAC TGTGCTCCTG ATGAACCGTA CATCCCAACG 
GAGTTGATAG ACAGTGTGGT GTCTGTAGCT GAAAACACTG CAGATGAATT GGCTCGAAGT 
GATGGCAGAG AAGTTCAGCT TGAGGAGGAT CCTGATCTGC AACTCCCTTT CCTTTTGCCT 
GAAGATGGTT ATTCATGTGA TGTGGTTAGA AATATTCCTA ATGGCCTTCA AGAGTTTTTA 
GAACCGCTCT GCCAGAGAGG TTTTTGCAGA CTTGTCCCTC ACTCACGGTC ACCAGGCATG 
TGGACAATTT ACTTTGAAGG AGCTGAATTT GAAAATAATT TGTTGACCGA CAATACAGAC 
CTGCCATTAA GAAAAGAACC TGAAATAATC ACTGTGACAC TTAAAAAGCA GAATGGAATG 
GGTTTGAGTA TTGTTGCAGC AAAGGGTGCT GGACAAGAAA AACTTGGGAT TTACATTAAG 
TCTGTGGTGA AAGGCGGTGC TGCAGAAGTG GATGGTCGTT TAGCTGCAGG GGACCAACTT 
ATAAGTGTGG ATGGGCAAAG TTTAGTTGGT TTGTCCCAAG AAAGAGCTGC AGAGCTTATG 
ACTAGAACTG GGGCCGTAGT AACCCTTGAG GTCGCAAAGC AAGGCGCAAT ATACCATGGA 
CTTGCCACAC TACTAAACCA ACCATCTCCT CTCATGCAAA GAGATCGACG TGGCTCAGGA 
AAACCACGAC CAAAGAGTGA AGGTTTTGAG CTTTACAATA ATGCTTCCCA AAATGGGTCT 
CCTGATAGCC CCCAGACACA ATGGCCAGAA TACACCGAAC CCAAAAAGTT ACCAGGCGAT 
GATAAACTAA TGAAAAACCG TGCAGATCAT CGATCCAGTC CTAATGTGGC CAATCAACCC 
CAGAGTCCAG GTGGAAAAAC TACTTATTCA AGCAGCTCAA CAGTGAAGAT AACATCTGTC 
TCCACTGGAA ACCTATGCAC TGAGGAACAG ACTGCACCAG CAAGACCAGA AGCTTATCCT 
ATCCCTACAC AAACATATGC AAGAGAGTAT TTTACTTTTC CAACTCCAAA ATCTCAAGAT 
AGAATAGGAC CAGCTCAGAA CCAATGGCAA GGCTATGAGG ATAATTTCCA AGTTCAGATG 
GGTAACAACC ATGCCAGAAA TACTTCGGCT CAACAAGTTG CTTATTCTGA GGAAGAGCTA 
AGGGATAATT TAACATTCCA GTCAGAAAGA GGGCGCGTTG AACCCCAAGT GCGGAAAGAT 
GTGGAACATC TGGATGGTAA TGATACTTGG TTCAATCGAG GCTCGTCTAT GGAATCAAGT 
GCATCTTGTC AAGACCTTAA TAATTCAAAG ACACATTCTG TAGGTTCTTT GAGCAAAACA 
GGACCTGGTC GCTGGAAAAC ACCAATTTCT TCTCAGTCAG CTACTGTTCT TTTGCCGCAG 
CCTGTTAGAA CAGACCTTCC TCCTCCACCA CCTCCACCAG TTCATTATGG AGCAGAATTT 
GAGGGAGTCC CAATAGATTT ACCTCTACCA CCCCCACCAT CTGAGAGCCA ACTAGGGCTT 
TCAACAAACT CACAGGCAGC TGCACTAGAA AGAAAGAAGC GGGAAGAACA TCAGAGGTGG 
TATGAGAAAG AGAAGGCACG TTTGGAAGAA GAAAGAGAAA GGAAAAGGCG AGAGCAGGAA 
AGAAAGCTAG GCCAGGTTCG TCCTCAGCCA CCAACTATAC CTCCACAACC AGTAAATCCA 
CAATCTCCTC CGTTGATTCC TCCTCCAAAT GTTGCACAAG CCAGAACAGA GAAACCTGCC 
ACTCTTCCTC GGCAACAAGA AACTGTTATC AGGGAGCTAC AACCTCAGCA GCAGCCACGA 
ACCATAGAAA GAAGAGACCT CCAGTACATT ACAGTCAGTA AAGAGGAGCT TTCTTCCAGT 
GACAGCCTTT CCCCAGATCC CTGGAAAAGG GATGCCAAGG AGAAGCTAGA AAAACAGCAG 
CAGATGCATA TAGTGGATAT GCTGAGCAAA GAGATCAATG AACTGCAAAA CAAGCCTGAC 
CGAACTTCTG AAGAAAATGA CCGACTCCGC AAATTAATGC TTGAATGGCA GTTTCAGAAG 
AGACTACAAG AGTCCCAGCA GAAGGATGAC GATGACGACG ATGACGATGA TGATGATGAT 
GATGTGGATA CCATGCTCAT CATGCAGCGT CTGGAAGCTG AAAGGAAAGC AAGGGATGAA 
GAACGGAGAC GGCAACAACA TTTGGAAGAG GCGCGCAAAC GGGAGGCAGA AGATCGCCTT 
AGGCAGGAAG AAGAGCATCG TCATCAAGAG GAGGAGCGGG CAAGGAGAGA GGCTGAAGAA 
AAGAGGAGAC AGGAAGAAGA GTACTACAAC CGCTTGGAAG CTGAGAGGCG CAGAATTCAT 
GAAGAAGCAG AGCGGCGATT GTTAGACCCT GAAGAGCCTG GTCTGTACAG ACCTCCACTC 
CCACGGGAAT ATGAGCTTTC CTCCCCAACC CTCCCACCTA ATCCTCCGCC TCCCCCTCCT 
CAGCGTAACA CCTCTTACCT CAAAACACAA GCCCTCTCCC CTGACACAGT TTACACTGCC 
AAGTTTGTTA CATACACTGA TGATGAGGAA GACACAAACC TATCAGGACC AAATTCTTAT 
TCTGGATCAT CTGGTGCAGC AGTTCCTGGT AATGAAGTTT ATAAAGATCC GAGGGATGTG 
CGACTAAAAA GCCAAGATGG AGAAGCTTAT AGCACTGGTG GAGCCCCAGA GAGCCTAACC 
TTTAAGGAGC GCCAGCGGCT CTTCTCTCAG GGCCAGGATG TGTCTAACAA AGTGAAAGCT 
TCCAGAAAAT TAATGGAACT GGAAAATGAG TTAAACACAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_031902300.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758160.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X9, mRNA.

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CloneID	OXa58619
Clone ID Related Accession (Same CDS sequence)	XM_031902300.1
Accession Version	XM_031902300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT 
ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG 
CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT 
CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT 
GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG 
CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT 
ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA 
ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT 
GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT 
AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT 
CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA 
AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG 
TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT 
GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT 
GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT 
TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA 
GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT 
GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA 
AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT 
GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT 
TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA 
ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA 
CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA 
GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA 
TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT 
GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT 
ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT 
GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA 
AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC 
CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT 
ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG 
TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT 
GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT 
AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA 
CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT 
CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA 
CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT 
GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT 
TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT 
CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT 
CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA 
ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC 
CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC 
TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG 
CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC 
CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG 
AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC 
ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGTTACAGG ATGAAGAACG GAGACGGCAA 
CAACATTTGG AAGAGGCGCG CAAACGGGAG GCAGAAGATC GCCTTAGGCA GGAAGAAGAG 
CATCGTCATC AAGAGGAGGA GCGGGCAAGG AGAGAGGCTG AAGAAAAGAG GAGACAGGAA 
GAAGAGTACT ACAACCGCTT GGAAGCTGAG AGGCGCAGAA TTCATGAAGA AGCAGAGCGG 
CGATTGTTAG ACCCTGAAGA GCCTGGTCTG TACAGACCTC CACTCCCACG GGAATATGAG 
CTTTCCTCCC CAACCCTCCC ACCTAATCCT CCGCCTCCCC CTCCTCAGCG TAACACCTCT 
TACCTCAAAA CACAAGCCCT CTCCCCTGAC ACAGTTTACA CTGCCAAGTT TGTTACATAC 
ACTGATGATG AGGAAGACAC AAACCTATCA GGACCAAATT CTTATTCTGG ATCATCTGGT 
GCAGCAGTTC CTGGTAATGA AGTTTATAAA GATCCGAGGG ATGTGCGACT AAAAAGCCAA 
GATGGAGAAG CTTATAGCAC TGGTGGAGCC CCAGAGAGCC TAACCTTTAA GGAGCGCCAG 
CGGCTCTTCT CTCAGGGCCA GGATGTGTCT AACAAAGTGA AAGCTTCCAG AAAATTAATG 
GAACTGGAAA ATGAGTTAAA CACAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758160.1
CDS	997..6426
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARLQDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902300.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902300.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT 
ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG 
CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT 
CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT 
GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG 
CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT 
ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA 
ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT 
GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT 
AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT 
CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA 
AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG 
TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT 
GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT 
GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT 
TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA 
GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT 
GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA 
AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT 
GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT 
TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA 
ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA 
CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA 
GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA 
TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT 
GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT 
ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT 
GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA 
AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC 
CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT 
ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG 
TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT 
GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT 
AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA 
CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT 
CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA 
CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT 
GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT 
TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT 
CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT 
CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA 
ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC 
CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC 
TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG 
CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC 
CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG 
AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC 
ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGTTACAGG ATGAAGAACG GAGACGGCAA 
CAACATTTGG AAGAGGCGCG CAAACGGGAG GCAGAAGATC GCCTTAGGCA GGAAGAAGAG 
CATCGTCATC AAGAGGAGGA GCGGGCAAGG AGAGAGGCTG AAGAAAAGAG GAGACAGGAA 
GAAGAGTACT ACAACCGCTT GGAAGCTGAG AGGCGCAGAA TTCATGAAGA AGCAGAGCGG 
CGATTGTTAG ACCCTGAAGA GCCTGGTCTG TACAGACCTC CACTCCCACG GGAATATGAG 
CTTTCCTCCC CAACCCTCCC ACCTAATCCT CCGCCTCCCC CTCCTCAGCG TAACACCTCT 
TACCTCAAAA CACAAGCCCT CTCCCCTGAC ACAGTTTACA CTGCCAAGTT TGTTACATAC 
ACTGATGATG AGGAAGACAC AAACCTATCA GGACCAAATT CTTATTCTGG ATCATCTGGT 
GCAGCAGTTC CTGGTAATGA AGTTTATAAA GATCCGAGGG ATGTGCGACT AAAAAGCCAA 
GATGGAGAAG CTTATAGCAC TGGTGGAGCC CCAGAGAGCC TAACCTTTAA GGAGCGCCAG 
CGGCTCTTCT CTCAGGGCCA GGATGTGTCT AACAAAGTGA AAGCTTCCAG AAAATTAATG 
GAACTGGAAA ATGAGTTAAA CACAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_031902301.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758161.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X10, mRNA.

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CloneID	OXa58620
Clone ID Related Accession (Same CDS sequence)	XM_031902301.1
Accession Version	XM_031902301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT 
CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA 
AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG 
TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA 
GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG 
GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT 
ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT 
GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA 
CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT 
TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA 
TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA 
GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG 
TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG 
GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT 
TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT 
TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG 
TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA 
GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT 
ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT 
GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA 
AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT 
GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA 
GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA 
CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT 
ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC 
AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT 
GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT 
GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGAAGCA AAAGAATATT 
GCTGGAGCTC TTGCTTTCTG GATGGCAAAC GCTTCAGAGC TTCTCAATTT CATAAAGCAA 
GACCGAGATC TCAATCGTAT CACATTGGAT GCACAAGATG TTCTTGCACA TTTAGTTCAG 
ATGGCATTCA AATACCTGGT TCACTGTCTT CAATCTGAAT TGACCAGCTC TATGCCTGCT 
TTTATAGATG ATCCAGAAGA AAATGATTCC CAAAGGCCTA AAATAGAAGA CGTTTTGCAT 
ACTCTGACAG GTGCCATGTC ACTTCTAAGA CGTTGCCGGG TCAACGCAGC ACTAACTATT 
CAGCTGTTCT CGCAGCTATT CCATTTCATC AACATGTGGC TGTTCAACAG GCTTGTAACA 
GATCCAGACT CTGGTTTATG CTCTCACTAT TGGGGGGCAA TAATTCGGCA GCAGCTTGCA 
CACATTGAGG CATGGGCTGA AAAACAAGGC CTCGAATTAG CTGCAGATTG TCATCTTAGC 
AGAATAGTTC AGGCTACTAC TCTTCTCACT ATGGACAAGT ACTCGCATCA GGATATTCCA 
AATATTAATA ATACATGTTT TAAGCTGAAT TCCTTACAAC TGCAAGCCCT ATTGCAAAAT 
TACCACTGTG CTCCTGATGA ACCGTACATC CCAACGGAGT TGATAGACAG TGTGGTGTCT 
GTAGCTGAAA ACACTGCAGA TGAATTGGCT CGAAGTGATG GCAGAGAAGT TCAGCTTGAG 
GAGGATCCTG ATCTGCAACT CCCTTTCCTT TTGCCTGAAG ATGGTTATTC ATGTGATGTG 
GTTAGAAATA TTCCTAATGG CCTTCAAGAG TTTTTAGAAC CGCTCTGCCA GAGAGGTTTT 
TGCAGACTTG TCCCTCACTC ACGGTCACCA GGCATGTGGA CAATTTACTT TGAAGGAGCT 
GAATTTGAAA ATAATTTGTT GACCGACAAT ACAGACCTGC CATTAAGAAA AGAACCTGAA 
ATAATCACTG TGACACTTAA AAAGCAGAAT GGAATGGGTT TGAGTATTGT TGCAGCAAAG 
GGTGCTGGAC AAGAAAAACT TGGGATTTAC ATTAAGTCTG TGGTGAAAGG CGGTGCTGCA 
GAAGTGGATG GTCGTTTAGC TGCAGGGGAC CAACTTATAA GTGTGGATGG GCAAAGTTTA 
GTTGGTTTGT CCCAAGAAAG AGCTGCAGAG CTTATGACTA GAACTGGGGC CGTAGTAACC 
CTTGAGGTCG CAAAGCAAGG CGCAATATAC CATGGACTTG CCACACTACT AAACCAACCA 
TCTCCTCTCA TGCAAAGAGA TCGACGTGGC TCAGGAAAAC CACGACCAAA GAGTGAAGGT 
TTTGAGCTTT ACAATAATGC TTCCCAAAAT GGGTCTCCTG ATAGCCCCCA GACACAATGG 
CCAGAATACA CCGAACCCAA AAAGTTACCA GGCGATGATA AACTAATGAA AAACCGTGCA 
GATCATCGAT CCAGTCCTAA TGTGGCCAAT CAACCCCAGA GTCCAGGTGG AAAAACTACT 
TATTCAAGCA GCTCAACAGT GAAGATAACA TCTGTCTCCA CTGGAAACCT ATGCACTGAG 
GAACAGACTG CACCAGCAAG ACCAGAAGCT TATCCTATCC CTACACAAAC ATATGCAAGA 
GAGTATTTTA CTTTTCCAAC TCCAAAATCT CAAGATAGAA TAGGACCAGC TCAGAACCAA 
TGGCAAGGCT ATGAGGATAA TTTCCAAGTT CAGATGGGTA ACAACCATGC CAGAAATACT 
TCGGCTCAAC AAGTTGCTTA TTCTGAGGAA GAGCTAAGGG ATAATTTAAC ATTCCAGTCA 
GAAAGAGGGC GCGTTGAACC CCAAGTGCGG AAAGATGTGG AACATCTGGA TGGTAATGAT 
ACTTGGTTCA ATCGAGGCTC GTCTATGGAA TCAAGTGCAT CTTGTCAAGA CCTTAATAAT 
TCAAAGACAC ATTCTGTAGG TTCTTTGAGC AAAACAGGAC CTGGTCGCTG GAAAACACCA 
ATTTCTTCTC AGTCAGCTAC TGTTCTTTTG CCGCAGCCTG TTAGAACAGA CCTTCCTCCT 
CCACCACCTC CACCAGTTCA TTATGGAGCA GAATTTGAGG GAGTCCCAAT AGATTTACCT 
CTACCACCCC CACCATCTGA GAGCCAACTA GGGCTTTCAA CAAACTCACA GGCAGCTGCA 
CTAGAAAGAA AGAAGCGGGA AGAACATCAG AGGTGGTATG AGAAAGAGAA GGCACGTTTG 
GAAGAAGAAA GAGAAAGGAA AAGGCGAGAG CAGGAAAGAA AGCTAGGCCA GGTTCGTCCT 
CAGCCACCAA CTATACCTCC ACAACCAGTA AATCCACAAT CTCCTCCGTT GATTCCTCCT 
CCAAATGTTG CACAAGCCAG AACAGAGAAA CCTGCCACTC TTCCTCGGCA ACAAGAAACT 
GTTATCAGGG AGCTACAACC TCAGCAGCAG CCACGAACCA TAGAAAGAAG AGACCTCCAG 
TACATTACAG TCAGTAAAGA GGAGCTTTCT TCCAGTGACA GCCTTTCCCC AGATCCCTGG 
AAAAGGGATG CCAAGGAGAA GCTAGAAAAA CAGCAGCAGA TGCATATAGT GGATATGCTG 
AGCAAAGAGA TCAATGAACT GCAAAACAAG CCTGACCGAA CTTCTGAAGA AAATGACCGA 
CTCCGCAAAT TAATGCTTGA ATGGCAGTTT CAGAAGAGAC TACAAGAGTC CCAGCAGAAG 
GATGACGATG ACGACGATGA CGATGATGAT GATGATGATG TGGATACCAT GCTCATCATG 
CAGCGTCTGG AAGCTGAAAG GAAAGCAAGG TTACAGGATG AAGAACGGAG ACGGCAACAA 
CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT 
CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA 
GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA 
TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT 
TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC 
CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT 
GATGATGAGG AAGACACAAA CCTATCAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA 
GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT 
GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG 
CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA 
CTGGAAAATG AGTTAAACAC AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758161.1
CDS	997..6423
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARLQDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902301.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902301.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT 
CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA 
AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG 
TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA 
GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG 
GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT 
ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT 
GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA 
CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT 
TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA 
TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA 
GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG 
TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG 
GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT 
TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT 
TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG 
TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA 
GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT 
ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT 
GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA 
AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT 
GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA 
GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA 
CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT 
ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC 
AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT 
GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT 
GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGAAGCA AAAGAATATT 
GCTGGAGCTC TTGCTTTCTG GATGGCAAAC GCTTCAGAGC TTCTCAATTT CATAAAGCAA 
GACCGAGATC TCAATCGTAT CACATTGGAT GCACAAGATG TTCTTGCACA TTTAGTTCAG 
ATGGCATTCA AATACCTGGT TCACTGTCTT CAATCTGAAT TGACCAGCTC TATGCCTGCT 
TTTATAGATG ATCCAGAAGA AAATGATTCC CAAAGGCCTA AAATAGAAGA CGTTTTGCAT 
ACTCTGACAG GTGCCATGTC ACTTCTAAGA CGTTGCCGGG TCAACGCAGC ACTAACTATT 
CAGCTGTTCT CGCAGCTATT CCATTTCATC AACATGTGGC TGTTCAACAG GCTTGTAACA 
GATCCAGACT CTGGTTTATG CTCTCACTAT TGGGGGGCAA TAATTCGGCA GCAGCTTGCA 
CACATTGAGG CATGGGCTGA AAAACAAGGC CTCGAATTAG CTGCAGATTG TCATCTTAGC 
AGAATAGTTC AGGCTACTAC TCTTCTCACT ATGGACAAGT ACTCGCATCA GGATATTCCA 
AATATTAATA ATACATGTTT TAAGCTGAAT TCCTTACAAC TGCAAGCCCT ATTGCAAAAT 
TACCACTGTG CTCCTGATGA ACCGTACATC CCAACGGAGT TGATAGACAG TGTGGTGTCT 
GTAGCTGAAA ACACTGCAGA TGAATTGGCT CGAAGTGATG GCAGAGAAGT TCAGCTTGAG 
GAGGATCCTG ATCTGCAACT CCCTTTCCTT TTGCCTGAAG ATGGTTATTC ATGTGATGTG 
GTTAGAAATA TTCCTAATGG CCTTCAAGAG TTTTTAGAAC CGCTCTGCCA GAGAGGTTTT 
TGCAGACTTG TCCCTCACTC ACGGTCACCA GGCATGTGGA CAATTTACTT TGAAGGAGCT 
GAATTTGAAA ATAATTTGTT GACCGACAAT ACAGACCTGC CATTAAGAAA AGAACCTGAA 
ATAATCACTG TGACACTTAA AAAGCAGAAT GGAATGGGTT TGAGTATTGT TGCAGCAAAG 
GGTGCTGGAC AAGAAAAACT TGGGATTTAC ATTAAGTCTG TGGTGAAAGG CGGTGCTGCA 
GAAGTGGATG GTCGTTTAGC TGCAGGGGAC CAACTTATAA GTGTGGATGG GCAAAGTTTA 
GTTGGTTTGT CCCAAGAAAG AGCTGCAGAG CTTATGACTA GAACTGGGGC CGTAGTAACC 
CTTGAGGTCG CAAAGCAAGG CGCAATATAC CATGGACTTG CCACACTACT AAACCAACCA 
TCTCCTCTCA TGCAAAGAGA TCGACGTGGC TCAGGAAAAC CACGACCAAA GAGTGAAGGT 
TTTGAGCTTT ACAATAATGC TTCCCAAAAT GGGTCTCCTG ATAGCCCCCA GACACAATGG 
CCAGAATACA CCGAACCCAA AAAGTTACCA GGCGATGATA AACTAATGAA AAACCGTGCA 
GATCATCGAT CCAGTCCTAA TGTGGCCAAT CAACCCCAGA GTCCAGGTGG AAAAACTACT 
TATTCAAGCA GCTCAACAGT GAAGATAACA TCTGTCTCCA CTGGAAACCT ATGCACTGAG 
GAACAGACTG CACCAGCAAG ACCAGAAGCT TATCCTATCC CTACACAAAC ATATGCAAGA 
GAGTATTTTA CTTTTCCAAC TCCAAAATCT CAAGATAGAA TAGGACCAGC TCAGAACCAA 
TGGCAAGGCT ATGAGGATAA TTTCCAAGTT CAGATGGGTA ACAACCATGC CAGAAATACT 
TCGGCTCAAC AAGTTGCTTA TTCTGAGGAA GAGCTAAGGG ATAATTTAAC ATTCCAGTCA 
GAAAGAGGGC GCGTTGAACC CCAAGTGCGG AAAGATGTGG AACATCTGGA TGGTAATGAT 
ACTTGGTTCA ATCGAGGCTC GTCTATGGAA TCAAGTGCAT CTTGTCAAGA CCTTAATAAT 
TCAAAGACAC ATTCTGTAGG TTCTTTGAGC AAAACAGGAC CTGGTCGCTG GAAAACACCA 
ATTTCTTCTC AGTCAGCTAC TGTTCTTTTG CCGCAGCCTG TTAGAACAGA CCTTCCTCCT 
CCACCACCTC CACCAGTTCA TTATGGAGCA GAATTTGAGG GAGTCCCAAT AGATTTACCT 
CTACCACCCC CACCATCTGA GAGCCAACTA GGGCTTTCAA CAAACTCACA GGCAGCTGCA 
CTAGAAAGAA AGAAGCGGGA AGAACATCAG AGGTGGTATG AGAAAGAGAA GGCACGTTTG 
GAAGAAGAAA GAGAAAGGAA AAGGCGAGAG CAGGAAAGAA AGCTAGGCCA GGTTCGTCCT 
CAGCCACCAA CTATACCTCC ACAACCAGTA AATCCACAAT CTCCTCCGTT GATTCCTCCT 
CCAAATGTTG CACAAGCCAG AACAGAGAAA CCTGCCACTC TTCCTCGGCA ACAAGAAACT 
GTTATCAGGG AGCTACAACC TCAGCAGCAG CCACGAACCA TAGAAAGAAG AGACCTCCAG 
TACATTACAG TCAGTAAAGA GGAGCTTTCT TCCAGTGACA GCCTTTCCCC AGATCCCTGG 
AAAAGGGATG CCAAGGAGAA GCTAGAAAAA CAGCAGCAGA TGCATATAGT GGATATGCTG 
AGCAAAGAGA TCAATGAACT GCAAAACAAG CCTGACCGAA CTTCTGAAGA AAATGACCGA 
CTCCGCAAAT TAATGCTTGA ATGGCAGTTT CAGAAGAGAC TACAAGAGTC CCAGCAGAAG 
GATGACGATG ACGACGATGA CGATGATGAT GATGATGATG TGGATACCAT GCTCATCATG 
CAGCGTCTGG AAGCTGAAAG GAAAGCAAGG TTACAGGATG AAGAACGGAG ACGGCAACAA 
CATTTGGAAG AGGCGCGCAA ACGGGAGGCA GAAGATCGCC TTAGGCAGGA AGAAGAGCAT 
CGTCATCAAG AGGAGGAGCG GGCAAGGAGA GAGGCTGAAG AAAAGAGGAG ACAGGAAGAA 
GAGTACTACA ACCGCTTGGA AGCTGAGAGG CGCAGAATTC ATGAAGAAGC AGAGCGGCGA 
TTGTTAGACC CTGAAGAGCC TGGTCTGTAC AGACCTCCAC TCCCACGGGA ATATGAGCTT 
TCCTCCCCAA CCCTCCCACC TAATCCTCCG CCTCCCCCTC CTCAGCGTAA CACCTCTTAC 
CTCAAAACAC AAGCCCTCTC CCCTGACACA GTTTACACTG CCAAGTTTGT TACATACACT 
GATGATGAGG AAGACACAAA CCTATCAGGA CCAAATTCTT ATTCTGGATC ATCTGGTGCA 
GCAGTTCCTG GTAATGAAGT TTATAAAGAT CCGAGGGATG TGCGACTAAA AAGCCAAGAT 
GGAGAAGCTT ATAGCACTGG TGGAGCCCCA GAGAGCCTAA CCTTTAAGGA GCGCCAGCGG 
CTCTTCTCTC AGGGCCAGGA TGTGTCTAAC AAAGTGAAAG CTTCCAGAAA ATTAATGGAA 
CTGGAAAATG AGTTAAACAC AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_031902302.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758162.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X11, mRNA.

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CloneID	OXa58621
Clone ID Related Accession (Same CDS sequence)	XM_031902302.1
Accession Version	XM_031902302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT 
CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA 
AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG 
TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA 
GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG 
GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT 
ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT 
GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA 
CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT 
TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA 
TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA 
GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG 
TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG 
GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT 
TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT 
TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG 
TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA 
GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT 
ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT 
GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA 
AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT 
GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA 
GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA 
CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT 
ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC 
AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT 
GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT 
GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGAAGCA AAAGAATATT 
GCTGGAGCTC TTGCTTTCTG GATGGCAAAC GCTTCAGAGC TTCTCAATTT CATAAAGCAA 
GACCGAGATC TCAATCGTAT CACATTGGAT GCACAAGATG TTCTTGCACA TTTAGTTCAG 
ATGGCATTCA AATACCTGGT TCACTGTCTT CAATCTGAAT TGACCAGCTC TATGCCTGCT 
TTTATAGATG ATCCAGAAGA AAATGATTCC CAAAGGCCTA AAATAGAAGA CGTTTTGCAT 
ACTCTGACAG GTGCCATGTC ACTTCTAAGA CGTTGCCGGG TCAACGCAGC ACTAACTATT 
CAGCTGTTCT CGCAGCTATT CCATTTCATC AACATGTGGC TGTTCAACAG GCTTGTAACA 
GATCCAGACT CTGGTTTATG CTCTCACTAT TGGGGGGCAA TAATTCGGCA GCAGCTTGCA 
CACATTGAGG CATGGGCTGA AAAACAAGGC CTCGAATTAG CTGCAGATTG TCATCTTAGC 
AGAATAGTTC AGGCTACTAC TCTTCTCACT ATGGACAAGT ACTCGCATCA GGATATTCCA 
AATATTAATA ATACATGTTT TAAGCTGAAT TCCTTACAAC TGCAAGCCCT ATTGCAAAAT 
TACCACTGTG CTCCTGATGA ACCGTACATC CCAACGGAGT TGATAGACAG TGTGGTGTCT 
GTAGCTGAAA ACACTGCAGA TGAATTGGCT CGAAGTGATG GCAGAGAAGT TCAGCTTGAG 
GAGGATCCTG ATCTGCAACT CCCTTTCCTT TTGCCTGAAG ATGGTTATTC ATGTGATGTG 
GTTAGAAATA TTCCTAATGG CCTTCAAGAG TTTTTAGAAC CGCTCTGCCA GAGAGGTTTT 
TGCAGACTTG TCCCTCACTC ACGGTCACCA GGCATGTGGA CAATTTACTT TGAAGGAGCT 
GAATTTGAAA ATAATTTGTT GACCGACAAT ACAGACCTGC CATTAAGAAA AGAACCTGAA 
ATAATCACTG TGACACTTAA AAAGCAGAAT GGAATGGGTT TGAGTATTGT TGCAGCAAAG 
GGTGCTGGAC AAGAAAAACT TGGGATTTAC ATTAAGTCTG TGGTGAAAGG CGGTGCTGCA 
GAAGTGGATG GTCGTTTAGC TGCAGGGGAC CAACTTATAA GTGTGGATGG GCAAAGTTTA 
GTTGGTTTGT CCCAAGAAAG AGCTGCAGAG CTTATGACTA GAACTGGGGC CGTAGTAACC 
CTTGAGGTCG CAAAGCAAGG CGCAATATAC CATGGACTTG CCACACTACT AAACCAACCA 
TCTCCTCTCA TGCAAAGAGA TCGACGTGGC TCAGGAAAAC CACGACCAAA GAGTGAAGGT 
TTTGAGCTTT ACAATAATGC TTCCCAAAAT GGGTCTCCTG ATAGCCCCCA GACACAATGG 
CCAGAATACA CCGAACCCAA AAAGTTACCA GGCGATGATA AACTAATGAA AAACCGTGCA 
GATCATCGAT CCAGTCCTAA TGTGGCCAAT CAACCCCAGA GTCCAGGTGG AAAAACTACT 
TATTCAAGCA GCTCAACAGT GAAGATAACA TCTGTCTCCA CTGGAAACCT ATGCACTGAG 
GAACAGACTG CACCAGCAAG ACCAGAAGCT TATCCTATCC CTACACAAAC ATATGCAAGA 
GAGTATTTTA CTTTTCCAAC TCCAAAATCT CAAGATAGAA TAGGACCAGC TCAGAACCAA 
TGGCAAGGCT ATGAGGATAA TTTCCAAGTT CAGATGGGTA ACAACCATGC CAGAAATACT 
TCGGCTCAAC AAGTTGCTTA TTCTGAGGAA GAGCTAAGGG ATAATTTAAC ATTCCAGTCA 
GAAAGAGGGC GCGTTGAACC CCAAGTGCGG AAAGATGTGG AACATCTGGA TGGTAATGAT 
ACTTGGTTCA ATCGAGGCTC GTCTATGGAA TCAAGTGCAT CTTGTCAAGA CCTTAATAAT 
TCAAAGACAC ATTCTGTAGG TTCTTTGAGC AAAACAGGAC CTGGTCGCTG GAAAACACCA 
ATTTCTTCTC AGTCAGCTAC TGTTCTTTTG CCGCAGCCTG TTAGAACAGA CCTTCCTCCT 
CCACCACCTC CACCAGTTCA TTATGGAGCA GAATTTGAGG GAGTCCCAAT AGATTTACCT 
CTACCACCCC CACCATCTGA GAGCCAACTA GGGCTTTCAA CAAACTCACA GGCAGCTGCA 
CTAGAAAGAA AGAAGCGGGA AGAACATCAG AGGTGGTATG AGAAAGAGAA GGCACGTTTG 
GAAGAAGAAA GAGAAAGGAA AAGGCGAGAG CAGGAAAGAA AGCTAGGCCA GGTTCGTCCT 
CAGCCACCAA CTATACCTCC ACAACCAGTA AATCCACAAT CTCCTCCGTT GATTCCTCCT 
CCAAATGTTG CACAAGCCAG AACAGAGAAA CCTGCCACTC TTCCTCGGCA ACAAGAAACT 
GTTATCAGGG AGCTACAACC TCAGCAGCAG CCACGAACCA TAGAAAGAAG AGACCTCCAG 
TACATTACAG TCAGTAAAGA GGAGCTTTCT TCCAGTGACA GCCTTTCCCC AGATCCCTGG 
AAAAGGGATG CCAAGGAGAA GCTAGAAAAA CAGCAGCAGA TGCATATAGT GGATATGCTG 
AGCAAAGAGA TCAATGAACT GCAAAACAAG CCTGACCGAA CTTCTGAAGA AAATGACCGA 
CTCCGCAAAT TAATGCTTGA ATGGCAGTTT CAGAAGAGAC TACAAGAGTC CCAGCAGAAG 
GATGACGATG ACGACGATGA CGATGATGAT GATGATGATG TGGATACCAT GCTCATCATG 
CAGCGTCTGG AAGCTGAAAG GAAAGCAAGG GATGAAGAAC GGAGACGGCA ACAACATTTG 
GAAGAGGCGC GCAAACGGGA GGCAGAAGAT CGCCTTAGGC AGGAAGAAGA GCATCGTCAT 
CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT GAAGAAAAGA GGAGACAGGA AGAAGAGTAC 
TACAACCGCT TGGAAGCTGA GAGGCGCAGA ATTCATGAAG AAGCAGAGCG GCGATTGTTA 
GACCCTGAAG AGCCTGGTCT GTACAGACCT CCACTCCCAC GGGAATATGA GCTTTCCTCC 
CCAACCCTCC CACCTAATCC TCCGCCTCCC CCTCCTCAGC GTAACACCTC TTACCTCAAA 
ACACAAGCCC TCTCCCCTGA CACAGTTTAC ACTGCCAAGT TTGTTACATA CACTGATGAT 
GAGGAAGACA CAAACCTATC AGGACCAAAT TCTTATTCTG GATCATCTGG TGCAGCAGTT 
CCTGGTAATG AAGTTTATAA AGATCCGAGG GATGTGCGAC TAAAAAGCCA AGATGGAGAA 
GCTTATAGCA CTGGTGGAGC CCCAGAGAGC CTAACCTTTA AGGAGCGCCA GCGGCTCTTC 
TCTCAGGGCC AGGATGTGTC TAACAAAGTG AAAGCTTCCA GAAAATTAAT GGAACTGGAA 
AATGAGTTAA ACACAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758162.1
CDS	999..6419
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902302.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902302.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
AGAAAACTTG ACTTTGAAGA GAAGCCTCTT GTGGTTCAAC TTAATTGGAA TAAGGACGAT 
CGGGAAGGAA GATTTGTTCT AAAGAATGAA AATGACATCT TTCCAGCTAA AAAGGCTCAA 
AGCAATGGAC CAGAGAAACA GGAGAAAGAG GGTGTAATCC AGAATTTTAA GAGGACCTTG 
TCAAAAAAGG AAAAAAAAGA GAAAAAAAGA CGTGAGAGGG ATATTTTGAA GCAGTCAACA 
GATAATGAAG ATAGGCCTCA AGTGAGAGAT GATAGTGAGA ACTTACAGTT AGCTATGGAG 
GTATACAAAG ATATGCCAGA GACCAGTTTT ACTCGCACAA TATCTAACCC AGAAGTGGTT 
ATGAAAAGAA GACGGCAGCA AAAATTGGAA AAAAGAATGC AAGAATTTCG CAGCTCTGAT 
GGACGACCAG ACTCGGGCGG GACATTGAGG ATTTATGCTG ACAGTTTAAA ACCAAATATA 
CCATACAAAA CAATCTTGTT GTCCACTAAT GATACCGCAG ATTTTGCTGT TATAGAAGCT 
TTAGAAAAAT ATGGAATGGA GAAAGAAAAC CCCAAGGAAT ATTGCATTGC TAAGGTTGTA 
TTGCCACCTG GAGCTCAGCA CTCAGACAAA GGCATGAAAG AATTTATTCT AGATGATGAA 
GAATGCCCTC TTCACATATT CAGGGATTGG CCAAGTGAGA AAGGTGAACT TGTGTTCCAG 
TTAAAAAGAC GGCCGACAGA TAGTCTTCTA AAGCAAAACC GAAAACATGA AAGCAAATCG 
GGAAAGAGCA AGGAGAGGGG CGACCACTAT TCTTTATCGC TTTCTCCTGA TAAACTGCCT 
TATTTGGTTG AGTTAAGTGC AGATGGATCA GACTCTAGAG ATAAGCCCAA GCTTTACCGT 
TTGCAACCGA GTGTCACAGA AGTTGGAACT GAAAAATTTC AGGATAATTC CATCCAGTTG 
TTTGGCCCAG GAATACAACA TCACCATTGT GACCTAAATA ACATGGATGG GGTAGTCACA 
GTTACTCCAA GAAGCATTGA TGCTGATACC TATGTAGATG GTCATCGTAT CTCTGAGACT 
ACTATGCTGC ACAGTGGAGT TACGGTTCAG TTTGGTTCTT CACATGTGTT CAAATTTGTT 
GATCCCAACC TTGATCACTT ACTGGGTAAA AGGCTTTCTG ATGCTGGGCA AATTGGTAAA 
AACCAAAGGA GCAAACAAGG GTTGCAAGAA ACTACTTTTG GGCTAGAAGG TGAAGTACTT 
GGAGATGTCA CATTGCCAGT AAATAAGATG GTCAGCAAAC TGGACAATGA CAGAACTCCA 
GGTATGACAG AGAGAGGAAT GGTGAAACCT ATAATAAGAA TGGACAAGCA ACAAGATCAA 
CGTGTGCAAA ATGGCAGATC TCAAGATTCC CAAGGACCAG AAAACGTGTT GCCTGCAAAT 
ATTGAATTTA GAGAAAATTC TGAAGATGCC TTTTTGTCTG CCATTATTAA CTATACAAAC 
AGTTCTACTG TGCATTTCAA ACTTTCACCA ACATATGTGC TATATATGAC CTGTCGGTAT 
GTATTGTCCA ACAAATACAG ACCAGATACA AGCCCTGCTG AGCGAACACA CAAGGTTATT 
GCTGTTGTGA ACAAGATGGT TGGAATGATG GAAAGTGTAA TCCAGAAGCA AAAGAATATT 
GCTGGAGCTC TTGCTTTCTG GATGGCAAAC GCTTCAGAGC TTCTCAATTT CATAAAGCAA 
GACCGAGATC TCAATCGTAT CACATTGGAT GCACAAGATG TTCTTGCACA TTTAGTTCAG 
ATGGCATTCA AATACCTGGT TCACTGTCTT CAATCTGAAT TGACCAGCTC TATGCCTGCT 
TTTATAGATG ATCCAGAAGA AAATGATTCC CAAAGGCCTA AAATAGAAGA CGTTTTGCAT 
ACTCTGACAG GTGCCATGTC ACTTCTAAGA CGTTGCCGGG TCAACGCAGC ACTAACTATT 
CAGCTGTTCT CGCAGCTATT CCATTTCATC AACATGTGGC TGTTCAACAG GCTTGTAACA 
GATCCAGACT CTGGTTTATG CTCTCACTAT TGGGGGGCAA TAATTCGGCA GCAGCTTGCA 
CACATTGAGG CATGGGCTGA AAAACAAGGC CTCGAATTAG CTGCAGATTG TCATCTTAGC 
AGAATAGTTC AGGCTACTAC TCTTCTCACT ATGGACAAGT ACTCGCATCA GGATATTCCA 
AATATTAATA ATACATGTTT TAAGCTGAAT TCCTTACAAC TGCAAGCCCT ATTGCAAAAT 
TACCACTGTG CTCCTGATGA ACCGTACATC CCAACGGAGT TGATAGACAG TGTGGTGTCT 
GTAGCTGAAA ACACTGCAGA TGAATTGGCT CGAAGTGATG GCAGAGAAGT TCAGCTTGAG 
GAGGATCCTG ATCTGCAACT CCCTTTCCTT TTGCCTGAAG ATGGTTATTC ATGTGATGTG 
GTTAGAAATA TTCCTAATGG CCTTCAAGAG TTTTTAGAAC CGCTCTGCCA GAGAGGTTTT 
TGCAGACTTG TCCCTCACTC ACGGTCACCA GGCATGTGGA CAATTTACTT TGAAGGAGCT 
GAATTTGAAA ATAATTTGTT GACCGACAAT ACAGACCTGC CATTAAGAAA AGAACCTGAA 
ATAATCACTG TGACACTTAA AAAGCAGAAT GGAATGGGTT TGAGTATTGT TGCAGCAAAG 
GGTGCTGGAC AAGAAAAACT TGGGATTTAC ATTAAGTCTG TGGTGAAAGG CGGTGCTGCA 
GAAGTGGATG GTCGTTTAGC TGCAGGGGAC CAACTTATAA GTGTGGATGG GCAAAGTTTA 
GTTGGTTTGT CCCAAGAAAG AGCTGCAGAG CTTATGACTA GAACTGGGGC CGTAGTAACC 
CTTGAGGTCG CAAAGCAAGG CGCAATATAC CATGGACTTG CCACACTACT AAACCAACCA 
TCTCCTCTCA TGCAAAGAGA TCGACGTGGC TCAGGAAAAC CACGACCAAA GAGTGAAGGT 
TTTGAGCTTT ACAATAATGC TTCCCAAAAT GGGTCTCCTG ATAGCCCCCA GACACAATGG 
CCAGAATACA CCGAACCCAA AAAGTTACCA GGCGATGATA AACTAATGAA AAACCGTGCA 
GATCATCGAT CCAGTCCTAA TGTGGCCAAT CAACCCCAGA GTCCAGGTGG AAAAACTACT 
TATTCAAGCA GCTCAACAGT GAAGATAACA TCTGTCTCCA CTGGAAACCT ATGCACTGAG 
GAACAGACTG CACCAGCAAG ACCAGAAGCT TATCCTATCC CTACACAAAC ATATGCAAGA 
GAGTATTTTA CTTTTCCAAC TCCAAAATCT CAAGATAGAA TAGGACCAGC TCAGAACCAA 
TGGCAAGGCT ATGAGGATAA TTTCCAAGTT CAGATGGGTA ACAACCATGC CAGAAATACT 
TCGGCTCAAC AAGTTGCTTA TTCTGAGGAA GAGCTAAGGG ATAATTTAAC ATTCCAGTCA 
GAAAGAGGGC GCGTTGAACC CCAAGTGCGG AAAGATGTGG AACATCTGGA TGGTAATGAT 
ACTTGGTTCA ATCGAGGCTC GTCTATGGAA TCAAGTGCAT CTTGTCAAGA CCTTAATAAT 
TCAAAGACAC ATTCTGTAGG TTCTTTGAGC AAAACAGGAC CTGGTCGCTG GAAAACACCA 
ATTTCTTCTC AGTCAGCTAC TGTTCTTTTG CCGCAGCCTG TTAGAACAGA CCTTCCTCCT 
CCACCACCTC CACCAGTTCA TTATGGAGCA GAATTTGAGG GAGTCCCAAT AGATTTACCT 
CTACCACCCC CACCATCTGA GAGCCAACTA GGGCTTTCAA CAAACTCACA GGCAGCTGCA 
CTAGAAAGAA AGAAGCGGGA AGAACATCAG AGGTGGTATG AGAAAGAGAA GGCACGTTTG 
GAAGAAGAAA GAGAAAGGAA AAGGCGAGAG CAGGAAAGAA AGCTAGGCCA GGTTCGTCCT 
CAGCCACCAA CTATACCTCC ACAACCAGTA AATCCACAAT CTCCTCCGTT GATTCCTCCT 
CCAAATGTTG CACAAGCCAG AACAGAGAAA CCTGCCACTC TTCCTCGGCA ACAAGAAACT 
GTTATCAGGG AGCTACAACC TCAGCAGCAG CCACGAACCA TAGAAAGAAG AGACCTCCAG 
TACATTACAG TCAGTAAAGA GGAGCTTTCT TCCAGTGACA GCCTTTCCCC AGATCCCTGG 
AAAAGGGATG CCAAGGAGAA GCTAGAAAAA CAGCAGCAGA TGCATATAGT GGATATGCTG 
AGCAAAGAGA TCAATGAACT GCAAAACAAG CCTGACCGAA CTTCTGAAGA AAATGACCGA 
CTCCGCAAAT TAATGCTTGA ATGGCAGTTT CAGAAGAGAC TACAAGAGTC CCAGCAGAAG 
GATGACGATG ACGACGATGA CGATGATGAT GATGATGATG TGGATACCAT GCTCATCATG 
CAGCGTCTGG AAGCTGAAAG GAAAGCAAGG GATGAAGAAC GGAGACGGCA ACAACATTTG 
GAAGAGGCGC GCAAACGGGA GGCAGAAGAT CGCCTTAGGC AGGAAGAAGA GCATCGTCAT 
CAAGAGGAGG AGCGGGCAAG GAGAGAGGCT GAAGAAAAGA GGAGACAGGA AGAAGAGTAC 
TACAACCGCT TGGAAGCTGA GAGGCGCAGA ATTCATGAAG AAGCAGAGCG GCGATTGTTA 
GACCCTGAAG AGCCTGGTCT GTACAGACCT CCACTCCCAC GGGAATATGA GCTTTCCTCC 
CCAACCCTCC CACCTAATCC TCCGCCTCCC CCTCCTCAGC GTAACACCTC TTACCTCAAA 
ACACAAGCCC TCTCCCCTGA CACAGTTTAC ACTGCCAAGT TTGTTACATA CACTGATGAT 
GAGGAAGACA CAAACCTATC AGGACCAAAT TCTTATTCTG GATCATCTGG TGCAGCAGTT 
CCTGGTAATG AAGTTTATAA AGATCCGAGG GATGTGCGAC TAAAAAGCCA AGATGGAGAA 
GCTTATAGCA CTGGTGGAGC CCCAGAGAGC CTAACCTTTA AGGAGCGCCA GCGGCTCTTC 
TCTCAGGGCC AGGATGTGTC TAACAAAGTG AAAGCTTCCA GAAAATTAAT GGAACTGGAA 
AATGAGTTAA ACACAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_031902303.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758163.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X12, mRNA.

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CloneID	OXa58622
Clone ID Related Accession (Same CDS sequence)	XM_031902303.1
Accession Version	XM_031902303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT 
ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG 
CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT 
CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT 
GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG 
CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT 
ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA 
ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT 
GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT 
AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT 
CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA 
AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG 
TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT 
GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT 
GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT 
TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA 
GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT 
GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA 
AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT 
GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT 
TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA 
ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA 
CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA 
GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA 
TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT 
GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT 
ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT 
GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA 
AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC 
CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT 
ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG 
TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT 
GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT 
AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA 
CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT 
CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA 
CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT 
GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT 
TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT 
CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT 
CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA 
ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC 
CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC 
TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG 
CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC 
CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG 
AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC 
ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGGATGAAG AACGGAGACG GCAACAACAT 
TTGGAAGAGG CGCGCAAACG GGAGGCAGAA GATCGCCTTA GGCAGGAAGA AGAGCATCGT 
CATCAAGAGG AGGAGCGGGC AAGGAGAGAG GCTGAAGAAA AGAGGAGACA GGAAGAAGAG 
TACTACAACC GCTTGGAAGC TGAGAGGCGC AGAATTCATG AAGAAGCAGA GCGGCGATTG 
TTAGACCCTG AAGAGCCTGG TCTGTACAGA CCTCCACTCC CACGGGAATA TGAGCTTTCC 
TCCCCAACCC TCCCACCTAA TCCTCCGCCT CCCCCTCCTC AGCGTAACAC CTCTTACCTC 
AAAACACAAG CCCTCTCCCC TGACACAGTT TACACTGCCA AGTTTGTTAC ATACACTGAT 
GATGAGGAAG ACACAAACCT ATCAGGACCA AATTCTTATT CTGGATCATC TGGTGCAGCA 
GTTCCTGGTA ATGAAGTTTA TAAAGATCCG AGGGATGTGC GACTAAAAAG CCAAGATGGA 
GAAGCTTATA GCACTGGTGG AGCCCCAGAG AGCCTAACCT TTAAGGAGCG CCAGCGGCTC 
TTCTCTCAGG GCCAGGATGT GTCTAACAAA GTGAAAGCTT CCAGAAAATT AATGGAACTG 
GAAAATGAGT TAAACACAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758163.1
CDS	998..6421
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_031902303.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902303.1

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACATGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAACGTGTGC AAAATGGCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT 
ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG 
CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT 
CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT 
GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG 
CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT 
ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA 
ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT 
GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT 
AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT 
CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA 
AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG 
TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT 
GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT 
GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT 
TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA 
GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT 
GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA 
AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT 
GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT 
TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA 
ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA 
CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA 
GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA 
TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT 
GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT 
ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT 
GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA 
AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC 
CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT 
ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG 
TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT 
GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT 
AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA 
CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT 
CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA 
CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT 
GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT 
TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT 
CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT 
CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA 
ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC 
CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC 
TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG 
CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC 
CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG 
AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC 
ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGGATGAAG AACGGAGACG GCAACAACAT 
TTGGAAGAGG CGCGCAAACG GGAGGCAGAA GATCGCCTTA GGCAGGAAGA AGAGCATCGT 
CATCAAGAGG AGGAGCGGGC AAGGAGAGAG GCTGAAGAAA AGAGGAGACA GGAAGAAGAG 
TACTACAACC GCTTGGAAGC TGAGAGGCGC AGAATTCATG AAGAAGCAGA GCGGCGATTG 
TTAGACCCTG AAGAGCCTGG TCTGTACAGA CCTCCACTCC CACGGGAATA TGAGCTTTCC 
TCCCCAACCC TCCCACCTAA TCCTCCGCCT CCCCCTCCTC AGCGTAACAC CTCTTACCTC 
AAAACACAAG CCCTCTCCCC TGACACAGTT TACACTGCCA AGTTTGTTAC ATACACTGAT 
GATGAGGAAG ACACAAACCT ATCAGGACCA AATTCTTATT CTGGATCATC TGGTGCAGCA 
GTTCCTGGTA ATGAAGTTTA TAAAGATCCG AGGGATGTGC GACTAAAAAG CCAAGATGGA 
GAAGCTTATA GCACTGGTGG AGCCCCAGAG AGCCTAACCT TTAAGGAGCG CCAGCGGCTC 
TTCTCTCAGG GCCAGGATGT GTCTAACAAA GTGAAAGCTT CCAGAAAATT AATGGAACTG 
GAAAATGAGT TAAACACAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_012963753.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819207.1 Xenopus tropicalis afadin, adherens junction formation factor (afdn), mRNA.

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CloneID	OXa35546
Clone ID Related Accession (Same CDS sequence)	XM_012963753.2
Accession Version	XM_012963753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	afadin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACACGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAATGGGAGT CAAACTTCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT 
ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG 
CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT 
CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT 
GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG 
CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT 
ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA 
ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT 
GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT 
AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT 
CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA 
AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG 
TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT 
GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT 
GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT 
TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA 
GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT 
GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA 
AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT 
GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT 
TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA 
ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA 
CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA 
GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA 
TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT 
GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT 
ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT 
GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA 
AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC 
CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT 
ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG 
TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT 
GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT 
AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA 
CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT 
CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA 
CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT 
GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT 
TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT 
CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT 
CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA 
ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC 
CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC 
TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG 
CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC 
CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG 
AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC 
ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGTTACAGG ATGAAGAACG GAGACGGCAA 
CAACATTTGG AAGAGGCGCG CAAACGGGAG GCAGAAGATC GCCTTAGGCA GGAAGAAGAG 
CATCGTCATC AAGAGGAGGA GCGGGCAAGG AGAGAGGCTG AAGAAAAGAG GAGACAGGAA 
GAAGAGTACT ACAACCGCTT GGAAGCTGAG AGGCGCAGAA TTCATGAAGA AGCAGAGCGG 
CGATTGTTAG ACCCTGAAGA GCCTGGTCTG TACAGACCTC CACTCCCACG GGAATATGAG 
CTTTCCTCCC CAACCCTCCC ACCTAATCCT CCGCCTCCCC CTCCTCAGCG TAACACCTCT 
TACCTCAAAA CACAAGCCCT CTCCCCTGAC ACAGTTTACA CTGCCAAGTT TGTTACATAC 
ACTGATGATG AGGAAGACAC AAACCTATCA GGACCAAATT CTTATTCTGG ATCATCTGGT 
GCAGCAGTTC CTGGTAATGA AGTTTATAAA GATCCGAGGG ATGTGCGACT AAAAAGCCAA 
GATGGAGAAG CTTATAGCAC TGGTGGAGCC CCAGAGAGCC TAACCTTTAA GGAGCGCCAG 
CGGCTCTTCT CTCAGGGCCA GGATGTGTCT AACAAAGTGA AAGCTTCCAG AAAATTAATG 
GAACTGGAAA ATGAGTTAAA CACAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819207.1
CDS	1316..6745
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSLSLSPDKLPYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMDGVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDPNLDHLLGKRLSDAGQIGKNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKLDNDRTPGMTERGMVKPIIRMDKQQDQWESNFRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFCRLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIYHGLATLLNQPSPLMQRDRRGSGKPRPKSEGFELYNNASQNGSPDSPQTQWPEYTEPKKLPGDDKLMKNRADHRSSPNVANQPQSPGGKTTYSSSSTVKITSVSTGNLCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQDRIGPAQNQWQGYEDNFQVQMGNNHARNTSAQQVAYSEEELRDNLTFQSERGRVEPQVRKDVEHLDGNDTWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWKTPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQAAALERKKREEHQRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNPQSPPLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQPRTIERRDLQYITVSKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRLQESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKARLQDEERRRQQHLEEARKREAEDRLRQEEEHRHQEEERARREAEEKRRQEEEYYNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPPNPPPPPPQRNTSYLKTQALSPDTVYTAKFVTYTDDEEDTNLSGPNSYSGSSGAAVPGNEVYKDPRDVRLKSQDGEAYSTGGAPESLTFKERQRLFSQGQDVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_012963753.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis afadin, adherens junction formation factor (afdn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963753.2

ORF Insert Sequence:

ATGTCGGGGA GTCGGGAAGA GGAGCGCCGC AAACTGGCAG ACATCATTAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CAGCCCACCG AGGATCTGGA GTTTCATGGA 
GTCATGCGCT TTTACTTTCA AGATAAAGCT GCTGGAAATT TTGCCACAAA ATGTATTAGA 
GTTTCCAGCA CTGCCACAAC ACAAGATGTC ATTGAAACTC TGGCAGAGAA ATTTCGTCCA 
GATATGAGAA TGCTGTCTTC CCCTAGATAC TCTTTGTATG AAGTTCATGT CAGTGGAGAA 
GAAAGAAAAC TTGACTTTGA AGAGAAGCCT CTTGTGGTTC AACTTAATTG GAATAAGGAC 
GATCGGGAAG GAAGATTTGT TCTAAAGAAT GAAAATGACA TCTTTCCAGC TAAAAAGGCT 
CAAAGCAATG GACCAGAGAA ACAGGAGAAA GAGGGTGTAA TCCAGAATTT TAAGAGGACC 
TTGTCAAAAA AGGAAAAAAA AGAGAAAAAA AGACGTGAGA GGGATATTTT GAAGCAGTCA 
ACAGATAATG AAGATAGGCC TCAAGTGAGA GATGATAGTG AGAACTTACA GTTAGCTATG 
GAGGTATACA AAGATATGCC AGAGACCAGT TTTACTCGCA CAATATCTAA CCCAGAAGTG 
GTTATGAAAA GAAGACGGCA GCAAAAATTG GAAAAAAGAA TGCAAGAATT TCGCAGCTCT 
GATGGACGAC CAGACTCGGG CGGGACATTG AGGATTTATG CTGACAGTTT AAAACCAAAT 
ATACCATACA AAACAATCTT GTTGTCCACT AATGATACCG CAGATTTTGC TGTTATAGAA 
GCTTTAGAAA AATATGGAAT GGAGAAAGAA AACCCCAAGG AATATTGCAT TGCTAAGGTT 
GTATTGCCAC CTGGAGCTCA GCACTCAGAC AAAGGCATGA AAGAATTTAT TCTAGATGAT 
GAAGAATGCC CTCTTCACAT ATTCAGGGAT TGGCCAAGTG AGAAAGGTGA ACTTGTGTTC 
CAGTTAAAAA GACGGCCGAC AGATAGTCTT CTAAAGCAAA ACCGAAAACA TGAAAGCAAA 
TCGGGAAAGA GCAAGGAGAG GGGCGACCAC TATTCTTTAT CGCTTTCTCC TGATAAACTG 
CCTTATTTGG TTGAGTTAAG TGCAGATGGA TCAGACTCTA GAGATAAGCC CAAGCTTTAC 
CGTTTGCAAC CGAGTGTCAC AGAAGTTGGA ACTGAAAAAT TTCAGGATAA TTCCATCCAG 
TTGTTTGGCC CAGGAATACA ACATCACCAT TGTGACCTAA ATAACATGGA TGGGGTAGTC 
ACAGTTACTC CAAGAAGCAT TGATGCTGAT ACCTATGTAG ATGGTCATCG TATCTCTGAG 
ACTACTATGC TGCACAGTGG AGTTACGGTT CAGTTTGGTT CTTCACACGT GTTCAAATTT 
GTTGATCCCA ACCTTGATCA CTTACTGGGT AAAAGGCTTT CTGATGCTGG GCAAATTGGT 
AAAAACCAAA GGAGCAAACA AGGGTTGCAA GAAACTACTT TTGGGCTAGA AGGTGAAGTA 
CTTGGAGATG TCACATTGCC AGTAAATAAG ATGGTCAGCA AACTGGACAA TGACAGAACT 
CCAGGTATGA CAGAGAGAGG AATGGTGAAA CCTATAATAA GAATGGACAA GCAACAAGAT 
CAATGGGAGT CAAACTTCAG ATCTCAAGAT TCCCAAGGAC CAGAAAACGT GTTGCCTGCA 
AATATTGAAT TTAGAGAAAA TTCTGAAGAT GCCTTTTTGT CTGCCATTAT TAACTATACA 
AACAGTTCTA CTGTGCATTT CAAACTTTCA CCAACATATG TGCTATATAT GACCTGTCGG 
TATGTATTGT CCAACAAATA CAGACCAGAT ACAAGCCCTG CTGAGCGAAC ACACAAGGTT 
ATTGCTGTTG TGAACAAGAT GGTTGGAATG ATGGAAAGTG TAATCCAGAA GCAAAAGAAT 
ATTGCTGGAG CTCTTGCTTT CTGGATGGCA AACGCTTCAG AGCTTCTCAA TTTCATAAAG 
CAAGACCGAG ATCTCAATCG TATCACATTG GATGCACAAG ATGTTCTTGC ACATTTAGTT 
CAGATGGCAT TCAAATACCT GGTTCACTGT CTTCAATCTG AATTGACCAG CTCTATGCCT 
GCTTTTATAG ATGATCCAGA AGAAAATGAT TCCCAAAGGC CTAAAATAGA AGACGTTTTG 
CATACTCTGA CAGGTGCCAT GTCACTTCTA AGACGTTGCC GGGTCAACGC AGCACTAACT 
ATTCAGCTGT TCTCGCAGCT ATTCCATTTC ATCAACATGT GGCTGTTCAA CAGGCTTGTA 
ACAGATCCAG ACTCTGGTTT ATGCTCTCAC TATTGGGGGG CAATAATTCG GCAGCAGCTT 
GCACACATTG AGGCATGGGC TGAAAAACAA GGCCTCGAAT TAGCTGCAGA TTGTCATCTT 
AGCAGAATAG TTCAGGCTAC TACTCTTCTC ACTATGGACA AGTACTCGCA TCAGGATATT 
CCAAATATTA ATAATACATG TTTTAAGCTG AATTCCTTAC AACTGCAAGC CCTATTGCAA 
AATTACCACT GTGCTCCTGA TGAACCGTAC ATCCCAACGG AGTTGATAGA CAGTGTGGTG 
TCTGTAGCTG AAAACACTGC AGATGAATTG GCTCGAAGTG ATGGCAGAGA AGTTCAGCTT 
GAGGAGGATC CTGATCTGCA ACTCCCTTTC CTTTTGCCTG AAGATGGTTA TTCATGTGAT 
GTGGTTAGAA ATATTCCTAA TGGCCTTCAA GAGTTTTTAG AACCGCTCTG CCAGAGAGGT 
TTTTGCAGAC TTGTCCCTCA CTCACGGTCA CCAGGCATGT GGACAATTTA CTTTGAAGGA 
GCTGAATTTG AAAATAATTT GTTGACCGAC AATACAGACC TGCCATTAAG AAAAGAACCT 
GAAATAATCA CTGTGACACT TAAAAAGCAG AATGGAATGG GTTTGAGTAT TGTTGCAGCA 
AAGGGTGCTG GACAAGAAAA ACTTGGGATT TACATTAAGT CTGTGGTGAA AGGCGGTGCT 
GCAGAAGTGG ATGGTCGTTT AGCTGCAGGG GACCAACTTA TAAGTGTGGA TGGGCAAAGT 
TTAGTTGGTT TGTCCCAAGA AAGAGCTGCA GAGCTTATGA CTAGAACTGG GGCCGTAGTA 
ACCCTTGAGG TCGCAAAGCA AGGCGCAATA TACCATGGAC TTGCCACACT ACTAAACCAA 
CCATCTCCTC TCATGCAAAG AGATCGACGT GGCTCAGGAA AACCACGACC AAAGAGTGAA 
GGTTTTGAGC TTTACAATAA TGCTTCCCAA AATGGGTCTC CTGATAGCCC CCAGACACAA 
TGGCCAGAAT ACACCGAACC CAAAAAGTTA CCAGGCGATG ATAAACTAAT GAAAAACCGT 
GCAGATCATC GATCCAGTCC TAATGTGGCC AATCAACCCC AGAGTCCAGG TGGAAAAACT 
ACTTATTCAA GCAGCTCAAC AGTGAAGATA ACATCTGTCT CCACTGGAAA CCTATGCACT 
GAGGAACAGA CTGCACCAGC AAGACCAGAA GCTTATCCTA TCCCTACACA AACATATGCA 
AGAGAGTATT TTACTTTTCC AACTCCAAAA TCTCAAGATA GAATAGGACC AGCTCAGAAC 
CAATGGCAAG GCTATGAGGA TAATTTCCAA GTTCAGATGG GTAACAACCA TGCCAGAAAT 
ACTTCGGCTC AACAAGTTGC TTATTCTGAG GAAGAGCTAA GGGATAATTT AACATTCCAG 
TCAGAAAGAG GGCGCGTTGA ACCCCAAGTG CGGAAAGATG TGGAACATCT GGATGGTAAT 
GATACTTGGT TCAATCGAGG CTCGTCTATG GAATCAAGTG CATCTTGTCA AGACCTTAAT 
AATTCAAAGA CACATTCTGT AGGTTCTTTG AGCAAAACAG GACCTGGTCG CTGGAAAACA 
CCAATTTCTT CTCAGTCAGC TACTGTTCTT TTGCCGCAGC CTGTTAGAAC AGACCTTCCT 
CCTCCACCAC CTCCACCAGT TCATTATGGA GCAGAATTTG AGGGAGTCCC AATAGATTTA 
CCTCTACCAC CCCCACCATC TGAGAGCCAA CTAGGGCTTT CAACAAACTC ACAGGCAGCT 
GCACTAGAAA GAAAGAAGCG GGAAGAACAT CAGAGGTGGT ATGAGAAAGA GAAGGCACGT 
TTGGAAGAAG AAAGAGAAAG GAAAAGGCGA GAGCAGGAAA GAAAGCTAGG CCAGGTTCGT 
CCTCAGCCAC CAACTATACC TCCACAACCA GTAAATCCAC AATCTCCTCC GTTGATTCCT 
CCTCCAAATG TTGCACAAGC CAGAACAGAG AAACCTGCCA CTCTTCCTCG GCAACAAGAA 
ACTGTTATCA GGGAGCTACA ACCTCAGCAG CAGCCACGAA CCATAGAAAG AAGAGACCTC 
CAGTACATTA CAGTCAGTAA AGAGGAGCTT TCTTCCAGTG ACAGCCTTTC CCCAGATCCC 
TGGAAAAGGG ATGCCAAGGA GAAGCTAGAA AAACAGCAGC AGATGCATAT AGTGGATATG 
CTGAGCAAAG AGATCAATGA ACTGCAAAAC AAGCCTGACC GAACTTCTGA AGAAAATGAC 
CGACTCCGCA AATTAATGCT TGAATGGCAG TTTCAGAAGA GACTACAAGA GTCCCAGCAG 
AAGGATGACG ATGACGACGA TGACGATGAT GATGATGATG ATGTGGATAC CATGCTCATC 
ATGCAGCGTC TGGAAGCTGA AAGGAAAGCA AGGTTACAGG ATGAAGAACG GAGACGGCAA 
CAACATTTGG AAGAGGCGCG CAAACGGGAG GCAGAAGATC GCCTTAGGCA GGAAGAAGAG 
CATCGTCATC AAGAGGAGGA GCGGGCAAGG AGAGAGGCTG AAGAAAAGAG GAGACAGGAA 
GAAGAGTACT ACAACCGCTT GGAAGCTGAG AGGCGCAGAA TTCATGAAGA AGCAGAGCGG 
CGATTGTTAG ACCCTGAAGA GCCTGGTCTG TACAGACCTC CACTCCCACG GGAATATGAG 
CTTTCCTCCC CAACCCTCCC ACCTAATCCT CCGCCTCCCC CTCCTCAGCG TAACACCTCT 
TACCTCAAAA CACAAGCCCT CTCCCCTGAC ACAGTTTACA CTGCCAAGTT TGTTACATAC 
ACTGATGATG AGGAAGACAC AAACCTATCA GGACCAAATT CTTATTCTGG ATCATCTGGT 
GCAGCAGTTC CTGGTAATGA AGTTTATAAA GATCCGAGGG ATGTGCGACT AAAAAGCCAA 
GATGGAGAAG CTTATAGCAC TGGTGGAGCC CCAGAGAGCC TAACCTTTAA GGAGCGCCAG 
CGGCTCTTCT CTCAGGGCCA GGATGTGTCT AACAAAGTGA AAGCTTCCAG AAAATTAATG 
GAACTGGAAA ATGAGTTAAA CACAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	afdn
Entrez Gene ID	100493083
Full Name	afadin, adherens junction formation factor
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Caenorhabditis elegans (roundworm)	afd-1	NP_001021660.1
Gallus gallus (chicken)	MLLT4	XP_004935593.1
Danio rerio (zebrafish)	mllt4	XP_686655.5
Homo sapiens (human)	MLLT4	NP_001193937.1
Pan troglodytes (chimpanzee)	MLLT4	XP_518857.4
	MLLT4	XP_001083153.1
Canis lupus familiaris (dog)	MLLT4	XP_005615632.1
Bos taurus (cattle)	MLLT4	XP_005211198.1
Mus musculus (house mouse)	Mllt4	NP_034936.1
Rattus norvegicus (Norway rat)	Mllt4	NP_037349.1
Xenopus tropicalis (tropical clawed frog)	mllt4	XP_002931548.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

afdn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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