sptb cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following sptb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sptb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa64305 XM_002936896.4
Latest version!
Xenopus tropicalis spectrin beta, erythrocytic (sptb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa64305 XM_004917264.4
Latest version!
Xenopus tropicalis spectrin beta, erythrocytic (sptb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa15023 XM_004917264.3
Latest version!
Xenopus tropicalis spectrin, beta, erythrocytic (sptb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa15023 XM_002936896.3
Latest version!
Xenopus tropicalis spectrin, beta, erythrocytic (sptb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa64305
    Clone ID Related Accession (Same CDS sequence) XM_002936896.4 , XM_004917264.4
    Accession Version XM_002936896.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin beta chain, erythrocytic
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936896.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGACCA CTGACTTTGA TAACCTGGAA ATTGAGCAGC AGTACTGCAC TATCAACTCC 
    CGCTGGGAAA CGGTAGAGGA TGACCTTGAC AATGATAACA GTTCCGCTCG AATGTTTGAA
    AGATCTAGAA TTAAAGCCCT TGCAGATGAG CGTGAAGCCG TGCAAAAGAA AACATTTACC
    AAATGGGTCA ACTCCCATCT TGCTTTTGTA AGTTGTCGCA TTTCTGATCT CTACTTGGAT
    CTGAGAGATG GAAGGATGTT AATAAAACTT TTGGAGGTGC TATCAGGAGA ACAGCTGCCA
    AGAAGAACAA AAGGGAGAAT GCGTATCCAC TGCTTAGAAA ATGTGGACAA AGCTCTTCAT
    TTTCTTAAGG AGCAAAGAGT CCATCTTGAA AATATTGGTC CTCATGATAT TGTCAACGGC
    AATCATCGAT TGGTATTGGG CCTTATATGG ACCATCATAT TACGTTTCCA GATCCAAGAT
    ATTATAATTG AAATGAAAGG CAGTGAAACT CGCTCTGCAA AGGATGCTTT GCTATTATGG
    TGCCAGATGA AGACAGCAGG GTATCCAAAT GTTAATGTGA CTAATTTCAC ATCAAGCTGG
    AGGGATGGAC TGGCATTCAA TGCGTTAATC CATAAACACA GGCCTGACCT GTTTGATTTT
    GAAAGAATGA AAAAATCCAA TGCAAAGCAC AACCTTGAGC ATGCCTTTGA TACAGCTGAT
    CACAAATTGG GAATAACTCA GCTCCTTGAT CCAGAAGATG TCCTTACCGA AAACCCAGAT
    GAAAAATCCA TAATCACCTA CGTAGTAGCA TTTTATCATT ATTTTTCAAA GATGAAGGCC
    TTGGCTGTAG AAGGAAAAAG AATTGGCAAG GTGTTGGATA ATGCGATTGA AACAGATAAA
    ATGATCTATG AATATGAAAG CCTGACATCG GATTTGTTGA CATGGATTGA ACAAACCATC
    TTAGCCCTTA TAAATCGAAC ATTTGCAAAC TCTTTAACTG GGGTCCAACA GCAGCTCCAG
    GCATTCAGTG CATACAGAAC TACAGAAAAA CCACCTAAAT TTCAAGAAAA GGGTTATTTG
    GAGGTGCTCT TATTCACCAT TCAATCTAGA ATGAGAGAAA ACAAACAGAA AGTATATGTC
    CCTCAAGATG GGAAGCTTGT TTCTGACATC AACCGGGCAT GGGGTCGCCT TGAAAAAGCT
    GAGCATGACC GTGAGACAGC ACTGAGAAAT GAACTTATTC GACAAGAAAA GCTTGAGCAA
    CTGGCAAAGC GGTTCGATCG CAAGGCTGCA ATGCGAGAAA CTTGGATTTC GGAAAACCAG
    CACCTCATTG CACAGGATAA TTTTGGTTTT GATTTGCCAT CAGTGGAAGC CGCCAAGAAG
    AAACATGAAG CTATTGAGAC GGACATCTAT GCATATGAAG AACGTATACA AGCACTAGTA
    AATGTAGCTA AGGAACTGGA GAATGAGAGA TACCATGATA TCAGGCGTAT TAGTGCCAGA
    AAGGACAACA TTGTGCGCCT GTGGCAGTAT TTACTGGAGA TACTGAAGTC TAGGCGCAAT
    AGACTTAACA TGACTGTTGA ACTGCAAACC TTGTTTCAAG AGATGCTGCA CATTGTAAAC
    TGGATGGATG AAATAAAGGG AGGCCTTTTA TCCCAAGATT ATGGTAAACA TCTCCTCGAG
    GTGGAATATT TACTGCAGAA ACACAGCTTG ACAGAAGCTG ATATTGCTGC TCAGGCGGAT
    AAAGTAAAAT CATTTAGCCA GGCTGCTCTG AAGTTTGTAG GTGGAGATGG GTATCAGCCA
    TGCGATCCAC AGGTGATTAA AGATCGAGTT CAGCACCTTG AACTTTGTTA TCAGGAACTA
    GTATCACTGG CAGCACAGAG GAGGCATAAT TTGCAGCAAT CACGTTGTTT GTGGAAGTTT
    TTTGGAGACA TTGAAGAGGT GGCAAGATGG ATAAGGGAGA AAGAACAAAT CTATACTTCA
    ATGGATTTTG GAAAAGACCT AACCAGCATC TCCATTCTCC AAAGAAAACA CAGGGCTTTT
    GAGGATGAAC TCAAAGGTCT TGAAACTAAT TTGCAGCACA CTCTTAAAGA TGGAGAACAA
    ATGATTGCCA ATAAACACTA TGCGTCACCG AAAATCAAAG AACAAATTGA AAATATCAAA
    GTTCAGTGGG CTCAGCTAAA GGATTTGGCA GCCTTCCGTT TGAAAATTCT TTGTGACACT
    GAGAACTTCT TTCAGTTCCA GGTTGATGCT GATGATCTGG CTGCAAGGAT GCAGGACACC
    TATCGTATGA TGCAAAGTGA CGATGTTGGT CATGATGAAT ATTCAACCAA AATATTAGTG
    AAAAAGCAAA AGGATCTCTT AGATGACATA ATGATCAATA GAAAGGTTCT GGAGGGTCTC
    AGTGAGGTGG TTCAAAGATT CCCAGAGGAG TTCAAGACTT CACCTGAGAT TGATAGTCGA
    CTACAAAAAT TAAGAGCTCT CTTTTCTGAT TTAGCATCCC TAGCAGACTT GCGTTCCCAA
    AAACTTCAAG ATGCTCTCTC ATTTTATACC GTTCTTGGTG ACATAGATGC ATGCGAGATG
    TGGATGAATG AAAAGGAGAA ATGGCTGGAA AAGATGGAGA TTCCAGAATC ACTAGAGGAC
    ATGGGAGTTG TACAGCATAG ATTTAATACA TTAGACCAAG AAATGGGTGC TCTGGGCTCC
    CAAATTCAGG ATGTGAACAC AGCAGCCAAT GCTCTTATTG AAAGTGGACA TCCCAGTAGG
    AAAAAAGTAA AGGAAGATCT GGATCACATG AATAGCAGAT GGACAGTGTT TCAAGAGATG
    GTTTCCCAAA AGCGAAGAGC AGTTGATTCT GCCCTTGGCC TTCACAATTA TGGTGTGGAA
    TGTGATGAGA CCAAGAACTG GATAATCGAA AAAATCAAGG TTGTAGAATC TATTCAGGAT
    CTTGGAAGTG ATCTTGCGTC TGTGATGTCA ATTCAAAGGA AACTCTATGG CATTGAGAGA
    GATCTAGCCG CTATTGAGGC CAAACTCATA AATCTACAAG GAGATGCACA AAAACTTGCA
    AATGATCACC CTGATCAAGC TCAAAACATC CTTGATCGAC TGATTGATAT AAATAAGAAA
    TGGGAAGAAT TGCAGAAGGC ACTGCAAAAT CAAGAGGAGT CCTTAGGAGA AAGTAGCAAA
    GTACAAAAAT TCTTGGTGGA CTTGTTTGAT TTTGAGACAT GGCTTTACAG AGCCCAACAG
    GCAACAGCTT CAGAAGATAT ACCCACTTCA CTGCCAGAGG CCGAAAAGCT ATATGCTAAC
    CACCTTTCTC TTAAAGATGA CATTGAACGC CATGAACCCG ATTTCTGTGA TGTTGTAGAT
    ACAGGTAACA AGGTAACCTC AGGACAAACA GACCCCCAAT ATGAACAACT TGATCAAAGA
    GGCAACGATA TAAAAGAAGG CTGGAATGAT CTCCACAAGA TGTGGGACAA TCGAGAGAAA
    TCCCTTTCTC AGTGCTTGGA ATTCCAGAAG TTCCTGAAGG ATGCAAAGCA GTCTGAAGCT
    ATTTTGAGTA ATCAGGAATA CACATTTGCT CATATTGAAG TTCCCACCAC ATTACAAGCT
    TCACAAACTA GACTGAAAAA ACATGAGGAT TTCCTAGCTA CTATGGAGAA TAATGACGAG
    AAGATTGTTG GAACCATCAA TCATGGAAAG AAATTAGATG AGGATAAAAA TGTTTTTGCT
    GATAAAGCTT TGGGCAAGAT TTCCTTTATT GAAGAAAGAT ATGCCAAGAA CTTAAACAAG
    GCCAAAGAAG TTTCTATTCT GCTGAAGGAT AACTGCGACC TCCAAAATTT CCTTGAAAAC
    TGCGAAGAGC TCAATATATG GATCAATGAA AAAATGTTGA CTGCTCAAGA CACATCTTAT
    GATGGAGCTC GAAACCTGCA CAGCAAATGG CTAAAGCACC AGGCTTTTAA GGCAGAACTT
    GATTCCAACA AAGAGAGACT TGAGAATCTT GACAAGGAAG GGAAACAACT GGCACAGGAA
    AAAGAACAGT TTGCACCAAT TATTTCTGAG AAACTGAGTG AGTTGCACAG ACTCTGGGAT
    GAACTGGAAA CTACCACCCT CCAAAAAGAA CAGTCTCTTT TTGATGCCCA CCGCACAGAG
    CTTTATGAGC AAAGATATGC AGATCTAAAT AAATGGATAA AAGATATGGA GCAGCAAGTA
    AAATCTGATG ACTGTGGAAC AGACCTTACA AGTGTTAATA TTCTACTAAA TAAGCACAAA
    AGAATTGAAG ATCAGATTCA ACAAAAGACA ATAGAAATGG ATCAAGTTCT ACCACAAGCT
    TCAGCTCTGG GCAAAGAACC TGATGAGAAG GAGCCAGAAA TTAACGTACG TTTTCTGCAA
    CTTGAGCCGC TAATGCAGGA GAGAAAGAGG AAACTTCTTT CTTCAAAGCA AGTACATCAA
    CTGCACCGGG ACTTGGAAGA TGAAATTCTT TGGATTCAGG AGAGATTGCC TCTTGCAGAG
    TCACAGGATT ATGGAAATAA CCTTCAGACG GTACAAATGC TAATGAAGAA AAATCAGACT
    TTACAGAAAG AGATTGCAGG TCACAGTCCC CGGATAGAGG ATGTTCTAAT GAGAGCAAAG
    CATATAGAAC ATGATGCAGA TATTGATGAT CAGCCTATTG AAAAGAAACG GAAGTCTTTG
    GAAGAGAGCT GGGATAACTT ACAGAAACAA ATAGCAGAAA GAGAAAAACA CTTGCTGCAA
    GCTCTTGAAG CACACCATTA TTATATGGAT GCAGGCGAGG CAGAGGCTTG GATTGATGAA
    CAACAGCTTT ACCTGATTGG AGATGAACAA CCAAAGGATG AAGAAAGTGT TGTTGCAATG
    TTGAAGAAAC ATCTCAATTT ACAACATGCC GTGGAACATT ATGGGAAGAA TATTAAGGAC
    TTGGCAGACC AGGCACAAAA ACTCCTTGCA TTAGAGCATC CTGATAGTGA ACGTATAACT
    CGGCACCAAG GCAATATAGA AAAGCAGTAC GCAGCACTGA GGGACATGGC TGATGAGAAA
    AGGCAAAAGC TGGACAATAT TTATCACATG TTTCAGCTGA GGCGTGAAGT TGAAGATCTG
    GAGCAATGGA TTGCAGAAAG AGATGTGAAG GCCTCATCCC AGGAAATGGG ACAAGATTTG
    GATCATGTGT CAATCCTGAG AGACAAATTC CGTGACTTTG CGAGAGAGAC AGGAGTCATT
    GGGCAGGAGC GTATTGATAA CGTCAATATT TTAATTGAAC AACTGATTGA TGCTGGCCAT
    GCAGATGCAG CAATGATTGC AGAATGGAAA GATAACTTAA ATGAGAGCTG GGCAGACTTG
    CTGGAACTTA TAGATACCCG TGTTCAGCTG CTGTCTGCAT CCTATGAACT CCATAAATTC
    TTCTATGATG GTACAGAAAT CCTAAGCATG ATTGAGGAGA AGCACAAGGA ATTGCCAGAA
    GATTTGGGGG GAGATGTACA CACAGCACAA TCACTTCACC GAGTGCATAC TGCATTTGAA
    AGAGATATTC ATTTTATTGG GAGCCAGGTA CAACACTTCC AAGACACAGC AGCACGTTTA
    TATGCTGCCT ATGCAGGAAC ACAGGCTGTG GATATACAGA AAAAGGAGCA AGAAGTTGTT
    GAAGCCTGGA AAGGCCTTCT GGATGCCTGT GATGGGCGTC GAACAGAGTT GATTAGTGCA
    GCAGAGAAAT TTCGGTTTTT CAGCATGGTG CGTGATTTGA TGCTTTGGAT GGAGAACATG
    ATTAGACAGA TTGAGACACA GGAAAAACCA AGAGATATAT CATCTGTTGA GTACCTAATA
    AAATACCACC AAGAACTAAA GGCAGAAATC GAAGCTAGAA ATGTCACATA TACAGCATGT
    GTGGATTTGG GAAACACTCT CTTGGCTCGT AATCACCCAG CTTCTGAGGA GATAAAAGAA
    AAGTTGGTGC AGCTAGAAGA ACGGAGAGAG GAGATGATGG AGAAGTGGCA CAAGCGCTGG
    GACTGGCTTA GTATTTTACT AGAGGTTTGC CAGTTTGCAA GAGACGCCTC CATGGCAGAA
    TCATGGTTAA TATCAAAGGA GCCATACCTG TACAGTACTT ATGTGGGAAA CAGCGTTGAT
    GATGTGGAAA AGTTAATCAG AAGGCATGAA TCATTTGAGA AATCCACTTT AACATGGGAA
    GAGAGATTTT CTGCGCTAGA AAGGCTAACA ACACTGGAGC TATTAGAAGT AAGGAAATAC
    CAGGAAGCTA AAAGACATGA AATGTTAATG GCTGCAGAAT CAGAAATGAA AGAGACAGCC
    CAGAAGGAAC AACAATTCCA AACACATGAA GAAAAAGAAC ATGAAGTGAA AGCCATTATC
    CATGCTGCCC CTGACTCTCC AGTGTTAGAG GAGCTAAAAC CAAGATTGCC CAGTATAACA
    GAAGTAACTG AAGAAACTAA GGAGAAAGAA GAGGAGAAGA TCACTCAGGA AGAGTATACA
    CATACCGAAT CCATGGAACA GACTGAATAT AGCATTTTAG ATCCAGAAAC CAGACAAATC
    GAAGCAACAA CTTTACCAAC AGATTCTGGT AGCTCTAGCA GTATTCAGCA TCAGGGTTAC
    CTAAACAGGA AACATGACCT TGATGGAACA CTTAAAAAAG CAGCAAACAG GTCCTGGAAC
    AATGTCTACT GTGTGCTTAA GAACCATGAT TTGTACTTTT ACAAAGATGC CAAATGCTTT
    GCTTTGAATG AAGCTTTCCA AGGTGAAGGC CCTCTGCCCC TGCGTAATGC AAGCTGTGAA
    ATAGTGGCCG ACTATAAAAA GAAAAAACAC GTCTTTAGGC TTCGACTCGG TGATGGAAAT
    GAATGGCTAT TTCAAGCGAA AGATGAGGCT GATCTTCAAA CCTGGGTGCC GATTCTTGCA
    ACAACCATTA CAGAGGCTAC AGATCATAAA CCTAAGTCCA AAAGTCTTCC TCTGCCCTCC
    ACATCTGCAT CTGAAGGAAA GCCAGAAAAG AAAGATAAAC GATTTACATT TTTCTCCTCA
    AAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002936942.2
    CDS166..7194
    Translation

    Target ORF information:

    RefSeq Version XM_002936896.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin beta, erythrocytic (sptb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002936896.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGACCA CTGACTTTGA TAACCTGGAA ATTGAGCAGC AGTACTGCAC TATCAACTCC 
    CGCTGGGAAA CGGTAGAGGA TGACCTTGAC AATGATAACA GTTCCGCTCG AATGTTTGAA
    AGATCTAGAA TTAAAGCCCT TGCAGATGAG CGTGAAGCCG TGCAAAAGAA AACATTTACC
    AAATGGGTCA ACTCCCATCT TGCTTTTGTA AGTTGTCGCA TTTCTGATCT CTACTTGGAT
    CTGAGAGATG GAAGGATGTT AATAAAACTT TTGGAGGTGC TATCAGGAGA ACAGCTGCCA
    AGAAGAACAA AAGGGAGAAT GCGTATCCAC TGCTTAGAAA ATGTGGACAA AGCTCTTCAT
    TTTCTTAAGG AGCAAAGAGT CCATCTTGAA AATATTGGTC CTCATGATAT TGTCAACGGC
    AATCATCGAT TGGTATTGGG CCTTATATGG ACCATCATAT TACGTTTCCA GATCCAAGAT
    ATTATAATTG AAATGAAAGG CAGTGAAACT CGCTCTGCAA AGGATGCTTT GCTATTATGG
    TGCCAGATGA AGACAGCAGG GTATCCAAAT GTTAATGTGA CTAATTTCAC ATCAAGCTGG
    AGGGATGGAC TGGCATTCAA TGCGTTAATC CATAAACACA GGCCTGACCT GTTTGATTTT
    GAAAGAATGA AAAAATCCAA TGCAAAGCAC AACCTTGAGC ATGCCTTTGA TACAGCTGAT
    CACAAATTGG GAATAACTCA GCTCCTTGAT CCAGAAGATG TCCTTACCGA AAACCCAGAT
    GAAAAATCCA TAATCACCTA CGTAGTAGCA TTTTATCATT ATTTTTCAAA GATGAAGGCC
    TTGGCTGTAG AAGGAAAAAG AATTGGCAAG GTGTTGGATA ATGCGATTGA AACAGATAAA
    ATGATCTATG AATATGAAAG CCTGACATCG GATTTGTTGA CATGGATTGA ACAAACCATC
    TTAGCCCTTA TAAATCGAAC ATTTGCAAAC TCTTTAACTG GGGTCCAACA GCAGCTCCAG
    GCATTCAGTG CATACAGAAC TACAGAAAAA CCACCTAAAT TTCAAGAAAA GGGTTATTTG
    GAGGTGCTCT TATTCACCAT TCAATCTAGA ATGAGAGAAA ACAAACAGAA AGTATATGTC
    CCTCAAGATG GGAAGCTTGT TTCTGACATC AACCGGGCAT GGGGTCGCCT TGAAAAAGCT
    GAGCATGACC GTGAGACAGC ACTGAGAAAT GAACTTATTC GACAAGAAAA GCTTGAGCAA
    CTGGCAAAGC GGTTCGATCG CAAGGCTGCA ATGCGAGAAA CTTGGATTTC GGAAAACCAG
    CACCTCATTG CACAGGATAA TTTTGGTTTT GATTTGCCAT CAGTGGAAGC CGCCAAGAAG
    AAACATGAAG CTATTGAGAC GGACATCTAT GCATATGAAG AACGTATACA AGCACTAGTA
    AATGTAGCTA AGGAACTGGA GAATGAGAGA TACCATGATA TCAGGCGTAT TAGTGCCAGA
    AAGGACAACA TTGTGCGCCT GTGGCAGTAT TTACTGGAGA TACTGAAGTC TAGGCGCAAT
    AGACTTAACA TGACTGTTGA ACTGCAAACC TTGTTTCAAG AGATGCTGCA CATTGTAAAC
    TGGATGGATG AAATAAAGGG AGGCCTTTTA TCCCAAGATT ATGGTAAACA TCTCCTCGAG
    GTGGAATATT TACTGCAGAA ACACAGCTTG ACAGAAGCTG ATATTGCTGC TCAGGCGGAT
    AAAGTAAAAT CATTTAGCCA GGCTGCTCTG AAGTTTGTAG GTGGAGATGG GTATCAGCCA
    TGCGATCCAC AGGTGATTAA AGATCGAGTT CAGCACCTTG AACTTTGTTA TCAGGAACTA
    GTATCACTGG CAGCACAGAG GAGGCATAAT TTGCAGCAAT CACGTTGTTT GTGGAAGTTT
    TTTGGAGACA TTGAAGAGGT GGCAAGATGG ATAAGGGAGA AAGAACAAAT CTATACTTCA
    ATGGATTTTG GAAAAGACCT AACCAGCATC TCCATTCTCC AAAGAAAACA CAGGGCTTTT
    GAGGATGAAC TCAAAGGTCT TGAAACTAAT TTGCAGCACA CTCTTAAAGA TGGAGAACAA
    ATGATTGCCA ATAAACACTA TGCGTCACCG AAAATCAAAG AACAAATTGA AAATATCAAA
    GTTCAGTGGG CTCAGCTAAA GGATTTGGCA GCCTTCCGTT TGAAAATTCT TTGTGACACT
    GAGAACTTCT TTCAGTTCCA GGTTGATGCT GATGATCTGG CTGCAAGGAT GCAGGACACC
    TATCGTATGA TGCAAAGTGA CGATGTTGGT CATGATGAAT ATTCAACCAA AATATTAGTG
    AAAAAGCAAA AGGATCTCTT AGATGACATA ATGATCAATA GAAAGGTTCT GGAGGGTCTC
    AGTGAGGTGG TTCAAAGATT CCCAGAGGAG TTCAAGACTT CACCTGAGAT TGATAGTCGA
    CTACAAAAAT TAAGAGCTCT CTTTTCTGAT TTAGCATCCC TAGCAGACTT GCGTTCCCAA
    AAACTTCAAG ATGCTCTCTC ATTTTATACC GTTCTTGGTG ACATAGATGC ATGCGAGATG
    TGGATGAATG AAAAGGAGAA ATGGCTGGAA AAGATGGAGA TTCCAGAATC ACTAGAGGAC
    ATGGGAGTTG TACAGCATAG ATTTAATACA TTAGACCAAG AAATGGGTGC TCTGGGCTCC
    CAAATTCAGG ATGTGAACAC AGCAGCCAAT GCTCTTATTG AAAGTGGACA TCCCAGTAGG
    AAAAAAGTAA AGGAAGATCT GGATCACATG AATAGCAGAT GGACAGTGTT TCAAGAGATG
    GTTTCCCAAA AGCGAAGAGC AGTTGATTCT GCCCTTGGCC TTCACAATTA TGGTGTGGAA
    TGTGATGAGA CCAAGAACTG GATAATCGAA AAAATCAAGG TTGTAGAATC TATTCAGGAT
    CTTGGAAGTG ATCTTGCGTC TGTGATGTCA ATTCAAAGGA AACTCTATGG CATTGAGAGA
    GATCTAGCCG CTATTGAGGC CAAACTCATA AATCTACAAG GAGATGCACA AAAACTTGCA
    AATGATCACC CTGATCAAGC TCAAAACATC CTTGATCGAC TGATTGATAT AAATAAGAAA
    TGGGAAGAAT TGCAGAAGGC ACTGCAAAAT CAAGAGGAGT CCTTAGGAGA AAGTAGCAAA
    GTACAAAAAT TCTTGGTGGA CTTGTTTGAT TTTGAGACAT GGCTTTACAG AGCCCAACAG
    GCAACAGCTT CAGAAGATAT ACCCACTTCA CTGCCAGAGG CCGAAAAGCT ATATGCTAAC
    CACCTTTCTC TTAAAGATGA CATTGAACGC CATGAACCCG ATTTCTGTGA TGTTGTAGAT
    ACAGGTAACA AGGTAACCTC AGGACAAACA GACCCCCAAT ATGAACAACT TGATCAAAGA
    GGCAACGATA TAAAAGAAGG CTGGAATGAT CTCCACAAGA TGTGGGACAA TCGAGAGAAA
    TCCCTTTCTC AGTGCTTGGA ATTCCAGAAG TTCCTGAAGG ATGCAAAGCA GTCTGAAGCT
    ATTTTGAGTA ATCAGGAATA CACATTTGCT CATATTGAAG TTCCCACCAC ATTACAAGCT
    TCACAAACTA GACTGAAAAA ACATGAGGAT TTCCTAGCTA CTATGGAGAA TAATGACGAG
    AAGATTGTTG GAACCATCAA TCATGGAAAG AAATTAGATG AGGATAAAAA TGTTTTTGCT
    GATAAAGCTT TGGGCAAGAT TTCCTTTATT GAAGAAAGAT ATGCCAAGAA CTTAAACAAG
    GCCAAAGAAG TTTCTATTCT GCTGAAGGAT AACTGCGACC TCCAAAATTT CCTTGAAAAC
    TGCGAAGAGC TCAATATATG GATCAATGAA AAAATGTTGA CTGCTCAAGA CACATCTTAT
    GATGGAGCTC GAAACCTGCA CAGCAAATGG CTAAAGCACC AGGCTTTTAA GGCAGAACTT
    GATTCCAACA AAGAGAGACT TGAGAATCTT GACAAGGAAG GGAAACAACT GGCACAGGAA
    AAAGAACAGT TTGCACCAAT TATTTCTGAG AAACTGAGTG AGTTGCACAG ACTCTGGGAT
    GAACTGGAAA CTACCACCCT CCAAAAAGAA CAGTCTCTTT TTGATGCCCA CCGCACAGAG
    CTTTATGAGC AAAGATATGC AGATCTAAAT AAATGGATAA AAGATATGGA GCAGCAAGTA
    AAATCTGATG ACTGTGGAAC AGACCTTACA AGTGTTAATA TTCTACTAAA TAAGCACAAA
    AGAATTGAAG ATCAGATTCA ACAAAAGACA ATAGAAATGG ATCAAGTTCT ACCACAAGCT
    TCAGCTCTGG GCAAAGAACC TGATGAGAAG GAGCCAGAAA TTAACGTACG TTTTCTGCAA
    CTTGAGCCGC TAATGCAGGA GAGAAAGAGG AAACTTCTTT CTTCAAAGCA AGTACATCAA
    CTGCACCGGG ACTTGGAAGA TGAAATTCTT TGGATTCAGG AGAGATTGCC TCTTGCAGAG
    TCACAGGATT ATGGAAATAA CCTTCAGACG GTACAAATGC TAATGAAGAA AAATCAGACT
    TTACAGAAAG AGATTGCAGG TCACAGTCCC CGGATAGAGG ATGTTCTAAT GAGAGCAAAG
    CATATAGAAC ATGATGCAGA TATTGATGAT CAGCCTATTG AAAAGAAACG GAAGTCTTTG
    GAAGAGAGCT GGGATAACTT ACAGAAACAA ATAGCAGAAA GAGAAAAACA CTTGCTGCAA
    GCTCTTGAAG CACACCATTA TTATATGGAT GCAGGCGAGG CAGAGGCTTG GATTGATGAA
    CAACAGCTTT ACCTGATTGG AGATGAACAA CCAAAGGATG AAGAAAGTGT TGTTGCAATG
    TTGAAGAAAC ATCTCAATTT ACAACATGCC GTGGAACATT ATGGGAAGAA TATTAAGGAC
    TTGGCAGACC AGGCACAAAA ACTCCTTGCA TTAGAGCATC CTGATAGTGA ACGTATAACT
    CGGCACCAAG GCAATATAGA AAAGCAGTAC GCAGCACTGA GGGACATGGC TGATGAGAAA
    AGGCAAAAGC TGGACAATAT TTATCACATG TTTCAGCTGA GGCGTGAAGT TGAAGATCTG
    GAGCAATGGA TTGCAGAAAG AGATGTGAAG GCCTCATCCC AGGAAATGGG ACAAGATTTG
    GATCATGTGT CAATCCTGAG AGACAAATTC CGTGACTTTG CGAGAGAGAC AGGAGTCATT
    GGGCAGGAGC GTATTGATAA CGTCAATATT TTAATTGAAC AACTGATTGA TGCTGGCCAT
    GCAGATGCAG CAATGATTGC AGAATGGAAA GATAACTTAA ATGAGAGCTG GGCAGACTTG
    CTGGAACTTA TAGATACCCG TGTTCAGCTG CTGTCTGCAT CCTATGAACT CCATAAATTC
    TTCTATGATG GTACAGAAAT CCTAAGCATG ATTGAGGAGA AGCACAAGGA ATTGCCAGAA
    GATTTGGGGG GAGATGTACA CACAGCACAA TCACTTCACC GAGTGCATAC TGCATTTGAA
    AGAGATATTC ATTTTATTGG GAGCCAGGTA CAACACTTCC AAGACACAGC AGCACGTTTA
    TATGCTGCCT ATGCAGGAAC ACAGGCTGTG GATATACAGA AAAAGGAGCA AGAAGTTGTT
    GAAGCCTGGA AAGGCCTTCT GGATGCCTGT GATGGGCGTC GAACAGAGTT GATTAGTGCA
    GCAGAGAAAT TTCGGTTTTT CAGCATGGTG CGTGATTTGA TGCTTTGGAT GGAGAACATG
    ATTAGACAGA TTGAGACACA GGAAAAACCA AGAGATATAT CATCTGTTGA GTACCTAATA
    AAATACCACC AAGAACTAAA GGCAGAAATC GAAGCTAGAA ATGTCACATA TACAGCATGT
    GTGGATTTGG GAAACACTCT CTTGGCTCGT AATCACCCAG CTTCTGAGGA GATAAAAGAA
    AAGTTGGTGC AGCTAGAAGA ACGGAGAGAG GAGATGATGG AGAAGTGGCA CAAGCGCTGG
    GACTGGCTTA GTATTTTACT AGAGGTTTGC CAGTTTGCAA GAGACGCCTC CATGGCAGAA
    TCATGGTTAA TATCAAAGGA GCCATACCTG TACAGTACTT ATGTGGGAAA CAGCGTTGAT
    GATGTGGAAA AGTTAATCAG AAGGCATGAA TCATTTGAGA AATCCACTTT AACATGGGAA
    GAGAGATTTT CTGCGCTAGA AAGGCTAACA ACACTGGAGC TATTAGAAGT AAGGAAATAC
    CAGGAAGCTA AAAGACATGA AATGTTAATG GCTGCAGAAT CAGAAATGAA AGAGACAGCC
    CAGAAGGAAC AACAATTCCA AACACATGAA GAAAAAGAAC ATGAAGTGAA AGCCATTATC
    CATGCTGCCC CTGACTCTCC AGTGTTAGAG GAGCTAAAAC CAAGATTGCC CAGTATAACA
    GAAGTAACTG AAGAAACTAA GGAGAAAGAA GAGGAGAAGA TCACTCAGGA AGAGTATACA
    CATACCGAAT CCATGGAACA GACTGAATAT AGCATTTTAG ATCCAGAAAC CAGACAAATC
    GAAGCAACAA CTTTACCAAC AGATTCTGGT AGCTCTAGCA GTATTCAGCA TCAGGGTTAC
    CTAAACAGGA AACATGACCT TGATGGAACA CTTAAAAAAG CAGCAAACAG GTCCTGGAAC
    AATGTCTACT GTGTGCTTAA GAACCATGAT TTGTACTTTT ACAAAGATGC CAAATGCTTT
    GCTTTGAATG AAGCTTTCCA AGGTGAAGGC CCTCTGCCCC TGCGTAATGC AAGCTGTGAA
    ATAGTGGCCG ACTATAAAAA GAAAAAACAC GTCTTTAGGC TTCGACTCGG TGATGGAAAT
    GAATGGCTAT TTCAAGCGAA AGATGAGGCT GATCTTCAAA CCTGGGTGCC GATTCTTGCA
    ACAACCATTA CAGAGGCTAC AGATCATAAA CCTAAGTCCA AAAGTCTTCC TCTGCCCTCC
    ACATCTGCAT CTGAAGGAAA GCCAGAAAAG AAAGATAAAC GATTTACATT TTTCTCCTCA
    AAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64305
    Clone ID Related Accession (Same CDS sequence) XM_002936896.4 , XM_004917264.4
    Accession Version XM_004917264.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin beta chain, erythrocytic
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917264.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGACCA CTGACTTTGA TAACCTGGAA ATTGAGCAGC AGTACTGCAC TATCAACTCC 
    CGCTGGGAAA CGGTAGAGGA TGACCTTGAC AATGATAACA GTTCCGCTCG AATGTTTGAA
    AGATCTAGAA TTAAAGCCCT TGCAGATGAG CGTGAAGCCG TGCAAAAGAA AACATTTACC
    AAATGGGTCA ACTCCCATCT TGCTTTTGTA AGTTGTCGCA TTTCTGATCT CTACTTGGAT
    CTGAGAGATG GAAGGATGTT AATAAAACTT TTGGAGGTGC TATCAGGAGA ACAGCTGCCA
    AGAAGAACAA AAGGGAGAAT GCGTATCCAC TGCTTAGAAA ATGTGGACAA AGCTCTTCAT
    TTTCTTAAGG AGCAAAGAGT CCATCTTGAA AATATTGGTC CTCATGATAT TGTCAACGGC
    AATCATCGAT TGGTATTGGG CCTTATATGG ACCATCATAT TACGTTTCCA GATCCAAGAT
    ATTATAATTG AAATGAAAGG CAGTGAAACT CGCTCTGCAA AGGATGCTTT GCTATTATGG
    TGCCAGATGA AGACAGCAGG GTATCCAAAT GTTAATGTGA CTAATTTCAC ATCAAGCTGG
    AGGGATGGAC TGGCATTCAA TGCGTTAATC CATAAACACA GGCCTGACCT GTTTGATTTT
    GAAAGAATGA AAAAATCCAA TGCAAAGCAC AACCTTGAGC ATGCCTTTGA TACAGCTGAT
    CACAAATTGG GAATAACTCA GCTCCTTGAT CCAGAAGATG TCCTTACCGA AAACCCAGAT
    GAAAAATCCA TAATCACCTA CGTAGTAGCA TTTTATCATT ATTTTTCAAA GATGAAGGCC
    TTGGCTGTAG AAGGAAAAAG AATTGGCAAG GTGTTGGATA ATGCGATTGA AACAGATAAA
    ATGATCTATG AATATGAAAG CCTGACATCG GATTTGTTGA CATGGATTGA ACAAACCATC
    TTAGCCCTTA TAAATCGAAC ATTTGCAAAC TCTTTAACTG GGGTCCAACA GCAGCTCCAG
    GCATTCAGTG CATACAGAAC TACAGAAAAA CCACCTAAAT TTCAAGAAAA GGGTTATTTG
    GAGGTGCTCT TATTCACCAT TCAATCTAGA ATGAGAGAAA ACAAACAGAA AGTATATGTC
    CCTCAAGATG GGAAGCTTGT TTCTGACATC AACCGGGCAT GGGGTCGCCT TGAAAAAGCT
    GAGCATGACC GTGAGACAGC ACTGAGAAAT GAACTTATTC GACAAGAAAA GCTTGAGCAA
    CTGGCAAAGC GGTTCGATCG CAAGGCTGCA ATGCGAGAAA CTTGGATTTC GGAAAACCAG
    CACCTCATTG CACAGGATAA TTTTGGTTTT GATTTGCCAT CAGTGGAAGC CGCCAAGAAG
    AAACATGAAG CTATTGAGAC GGACATCTAT GCATATGAAG AACGTATACA AGCACTAGTA
    AATGTAGCTA AGGAACTGGA GAATGAGAGA TACCATGATA TCAGGCGTAT TAGTGCCAGA
    AAGGACAACA TTGTGCGCCT GTGGCAGTAT TTACTGGAGA TACTGAAGTC TAGGCGCAAT
    AGACTTAACA TGACTGTTGA ACTGCAAACC TTGTTTCAAG AGATGCTGCA CATTGTAAAC
    TGGATGGATG AAATAAAGGG AGGCCTTTTA TCCCAAGATT ATGGTAAACA TCTCCTCGAG
    GTGGAATATT TACTGCAGAA ACACAGCTTG ACAGAAGCTG ATATTGCTGC TCAGGCGGAT
    AAAGTAAAAT CATTTAGCCA GGCTGCTCTG AAGTTTGTAG GTGGAGATGG GTATCAGCCA
    TGCGATCCAC AGGTGATTAA AGATCGAGTT CAGCACCTTG AACTTTGTTA TCAGGAACTA
    GTATCACTGG CAGCACAGAG GAGGCATAAT TTGCAGCAAT CACGTTGTTT GTGGAAGTTT
    TTTGGAGACA TTGAAGAGGT GGCAAGATGG ATAAGGGAGA AAGAACAAAT CTATACTTCA
    ATGGATTTTG GAAAAGACCT AACCAGCATC TCCATTCTCC AAAGAAAACA CAGGGCTTTT
    GAGGATGAAC TCAAAGGTCT TGAAACTAAT TTGCAGCACA CTCTTAAAGA TGGAGAACAA
    ATGATTGCCA ATAAACACTA TGCGTCACCG AAAATCAAAG AACAAATTGA AAATATCAAA
    GTTCAGTGGG CTCAGCTAAA GGATTTGGCA GCCTTCCGTT TGAAAATTCT TTGTGACACT
    GAGAACTTCT TTCAGTTCCA GGTTGATGCT GATGATCTGG CTGCAAGGAT GCAGGACACC
    TATCGTATGA TGCAAAGTGA CGATGTTGGT CATGATGAAT ATTCAACCAA AATATTAGTG
    AAAAAGCAAA AGGATCTCTT AGATGACATA ATGATCAATA GAAAGGTTCT GGAGGGTCTC
    AGTGAGGTGG TTCAAAGATT CCCAGAGGAG TTCAAGACTT CACCTGAGAT TGATAGTCGA
    CTACAAAAAT TAAGAGCTCT CTTTTCTGAT TTAGCATCCC TAGCAGACTT GCGTTCCCAA
    AAACTTCAAG ATGCTCTCTC ATTTTATACC GTTCTTGGTG ACATAGATGC ATGCGAGATG
    TGGATGAATG AAAAGGAGAA ATGGCTGGAA AAGATGGAGA TTCCAGAATC ACTAGAGGAC
    ATGGGAGTTG TACAGCATAG ATTTAATACA TTAGACCAAG AAATGGGTGC TCTGGGCTCC
    CAAATTCAGG ATGTGAACAC AGCAGCCAAT GCTCTTATTG AAAGTGGACA TCCCAGTAGG
    AAAAAAGTAA AGGAAGATCT GGATCACATG AATAGCAGAT GGACAGTGTT TCAAGAGATG
    GTTTCCCAAA AGCGAAGAGC AGTTGATTCT GCCCTTGGCC TTCACAATTA TGGTGTGGAA
    TGTGATGAGA CCAAGAACTG GATAATCGAA AAAATCAAGG TTGTAGAATC TATTCAGGAT
    CTTGGAAGTG ATCTTGCGTC TGTGATGTCA ATTCAAAGGA AACTCTATGG CATTGAGAGA
    GATCTAGCCG CTATTGAGGC CAAACTCATA AATCTACAAG GAGATGCACA AAAACTTGCA
    AATGATCACC CTGATCAAGC TCAAAACATC CTTGATCGAC TGATTGATAT AAATAAGAAA
    TGGGAAGAAT TGCAGAAGGC ACTGCAAAAT CAAGAGGAGT CCTTAGGAGA AAGTAGCAAA
    GTACAAAAAT TCTTGGTGGA CTTGTTTGAT TTTGAGACAT GGCTTTACAG AGCCCAACAG
    GCAACAGCTT CAGAAGATAT ACCCACTTCA CTGCCAGAGG CCGAAAAGCT ATATGCTAAC
    CACCTTTCTC TTAAAGATGA CATTGAACGC CATGAACCCG ATTTCTGTGA TGTTGTAGAT
    ACAGGTAACA AGGTAACCTC AGGACAAACA GACCCCCAAT ATGAACAACT TGATCAAAGA
    GGCAACGATA TAAAAGAAGG CTGGAATGAT CTCCACAAGA TGTGGGACAA TCGAGAGAAA
    TCCCTTTCTC AGTGCTTGGA ATTCCAGAAG TTCCTGAAGG ATGCAAAGCA GTCTGAAGCT
    ATTTTGAGTA ATCAGGAATA CACATTTGCT CATATTGAAG TTCCCACCAC ATTACAAGCT
    TCACAAACTA GACTGAAAAA ACATGAGGAT TTCCTAGCTA CTATGGAGAA TAATGACGAG
    AAGATTGTTG GAACCATCAA TCATGGAAAG AAATTAGATG AGGATAAAAA TGTTTTTGCT
    GATAAAGCTT TGGGCAAGAT TTCCTTTATT GAAGAAAGAT ATGCCAAGAA CTTAAACAAG
    GCCAAAGAAG TTTCTATTCT GCTGAAGGAT AACTGCGACC TCCAAAATTT CCTTGAAAAC
    TGCGAAGAGC TCAATATATG GATCAATGAA AAAATGTTGA CTGCTCAAGA CACATCTTAT
    GATGGAGCTC GAAACCTGCA CAGCAAATGG CTAAAGCACC AGGCTTTTAA GGCAGAACTT
    GATTCCAACA AAGAGAGACT TGAGAATCTT GACAAGGAAG GGAAACAACT GGCACAGGAA
    AAAGAACAGT TTGCACCAAT TATTTCTGAG AAACTGAGTG AGTTGCACAG ACTCTGGGAT
    GAACTGGAAA CTACCACCCT CCAAAAAGAA CAGTCTCTTT TTGATGCCCA CCGCACAGAG
    CTTTATGAGC AAAGATATGC AGATCTAAAT AAATGGATAA AAGATATGGA GCAGCAAGTA
    AAATCTGATG ACTGTGGAAC AGACCTTACA AGTGTTAATA TTCTACTAAA TAAGCACAAA
    AGAATTGAAG ATCAGATTCA ACAAAAGACA ATAGAAATGG ATCAAGTTCT ACCACAAGCT
    TCAGCTCTGG GCAAAGAACC TGATGAGAAG GAGCCAGAAA TTAACGTACG TTTTCTGCAA
    CTTGAGCCGC TAATGCAGGA GAGAAAGAGG AAACTTCTTT CTTCAAAGCA AGTACATCAA
    CTGCACCGGG ACTTGGAAGA TGAAATTCTT TGGATTCAGG AGAGATTGCC TCTTGCAGAG
    TCACAGGATT ATGGAAATAA CCTTCAGACG GTACAAATGC TAATGAAGAA AAATCAGACT
    TTACAGAAAG AGATTGCAGG TCACAGTCCC CGGATAGAGG ATGTTCTAAT GAGAGCAAAG
    CATATAGAAC ATGATGCAGA TATTGATGAT CAGCCTATTG AAAAGAAACG GAAGTCTTTG
    GAAGAGAGCT GGGATAACTT ACAGAAACAA ATAGCAGAAA GAGAAAAACA CTTGCTGCAA
    GCTCTTGAAG CACACCATTA TTATATGGAT GCAGGCGAGG CAGAGGCTTG GATTGATGAA
    CAACAGCTTT ACCTGATTGG AGATGAACAA CCAAAGGATG AAGAAAGTGT TGTTGCAATG
    TTGAAGAAAC ATCTCAATTT ACAACATGCC GTGGAACATT ATGGGAAGAA TATTAAGGAC
    TTGGCAGACC AGGCACAAAA ACTCCTTGCA TTAGAGCATC CTGATAGTGA ACGTATAACT
    CGGCACCAAG GCAATATAGA AAAGCAGTAC GCAGCACTGA GGGACATGGC TGATGAGAAA
    AGGCAAAAGC TGGACAATAT TTATCACATG TTTCAGCTGA GGCGTGAAGT TGAAGATCTG
    GAGCAATGGA TTGCAGAAAG AGATGTGAAG GCCTCATCCC AGGAAATGGG ACAAGATTTG
    GATCATGTGT CAATCCTGAG AGACAAATTC CGTGACTTTG CGAGAGAGAC AGGAGTCATT
    GGGCAGGAGC GTATTGATAA CGTCAATATT TTAATTGAAC AACTGATTGA TGCTGGCCAT
    GCAGATGCAG CAATGATTGC AGAATGGAAA GATAACTTAA ATGAGAGCTG GGCAGACTTG
    CTGGAACTTA TAGATACCCG TGTTCAGCTG CTGTCTGCAT CCTATGAACT CCATAAATTC
    TTCTATGATG GTACAGAAAT CCTAAGCATG ATTGAGGAGA AGCACAAGGA ATTGCCAGAA
    GATTTGGGGG GAGATGTACA CACAGCACAA TCACTTCACC GAGTGCATAC TGCATTTGAA
    AGAGATATTC ATTTTATTGG GAGCCAGGTA CAACACTTCC AAGACACAGC AGCACGTTTA
    TATGCTGCCT ATGCAGGAAC ACAGGCTGTG GATATACAGA AAAAGGAGCA AGAAGTTGTT
    GAAGCCTGGA AAGGCCTTCT GGATGCCTGT GATGGGCGTC GAACAGAGTT GATTAGTGCA
    GCAGAGAAAT TTCGGTTTTT CAGCATGGTG CGTGATTTGA TGCTTTGGAT GGAGAACATG
    ATTAGACAGA TTGAGACACA GGAAAAACCA AGAGATATAT CATCTGTTGA GTACCTAATA
    AAATACCACC AAGAACTAAA GGCAGAAATC GAAGCTAGAA ATGTCACATA TACAGCATGT
    GTGGATTTGG GAAACACTCT CTTGGCTCGT AATCACCCAG CTTCTGAGGA GATAAAAGAA
    AAGTTGGTGC AGCTAGAAGA ACGGAGAGAG GAGATGATGG AGAAGTGGCA CAAGCGCTGG
    GACTGGCTTA GTATTTTACT AGAGGTTTGC CAGTTTGCAA GAGACGCCTC CATGGCAGAA
    TCATGGTTAA TATCAAAGGA GCCATACCTG TACAGTACTT ATGTGGGAAA CAGCGTTGAT
    GATGTGGAAA AGTTAATCAG AAGGCATGAA TCATTTGAGA AATCCACTTT AACATGGGAA
    GAGAGATTTT CTGCGCTAGA AAGGCTAACA ACACTGGAGC TATTAGAAGT AAGGAAATAC
    CAGGAAGCTA AAAGACATGA AATGTTAATG GCTGCAGAAT CAGAAATGAA AGAGACAGCC
    CAGAAGGAAC AACAATTCCA AACACATGAA GAAAAAGAAC ATGAAGTGAA AGCCATTATC
    CATGCTGCCC CTGACTCTCC AGTGTTAGAG GAGCTAAAAC CAAGATTGCC CAGTATAACA
    GAAGTAACTG AAGAAACTAA GGAGAAAGAA GAGGAGAAGA TCACTCAGGA AGAGTATACA
    CATACCGAAT CCATGGAACA GACTGAATAT AGCATTTTAG ATCCAGAAAC CAGACAAATC
    GAAGCAACAA CTTTACCAAC AGATTCTGGT AGCTCTAGCA GTATTCAGCA TCAGGGTTAC
    CTAAACAGGA AACATGACCT TGATGGAACA CTTAAAAAAG CAGCAAACAG GTCCTGGAAC
    AATGTCTACT GTGTGCTTAA GAACCATGAT TTGTACTTTT ACAAAGATGC CAAATGCTTT
    GCTTTGAATG AAGCTTTCCA AGGTGAAGGC CCTCTGCCCC TGCGTAATGC AAGCTGTGAA
    ATAGTGGCCG ACTATAAAAA GAAAAAACAC GTCTTTAGGC TTCGACTCGG TGATGGAAAT
    GAATGGCTAT TTCAAGCGAA AGATGAGGCT GATCTTCAAA CCTGGGTGCC GATTCTTGCA
    ACAACCATTA CAGAGGCTAC AGATCATAAA CCTAAGTCCA AAAGTCTTCC TCTGCCCTCC
    ACATCTGCAT CTGAAGGAAA GCCAGAAAAG AAAGATAAAC GATTTACATT TTTCTCCTCA
    AAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917321.1
    CDS135..7163
    Translation

    Target ORF information:

    RefSeq Version XM_004917264.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin beta, erythrocytic (sptb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917264.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGACCA CTGACTTTGA TAACCTGGAA ATTGAGCAGC AGTACTGCAC TATCAACTCC 
    CGCTGGGAAA CGGTAGAGGA TGACCTTGAC AATGATAACA GTTCCGCTCG AATGTTTGAA
    AGATCTAGAA TTAAAGCCCT TGCAGATGAG CGTGAAGCCG TGCAAAAGAA AACATTTACC
    AAATGGGTCA ACTCCCATCT TGCTTTTGTA AGTTGTCGCA TTTCTGATCT CTACTTGGAT
    CTGAGAGATG GAAGGATGTT AATAAAACTT TTGGAGGTGC TATCAGGAGA ACAGCTGCCA
    AGAAGAACAA AAGGGAGAAT GCGTATCCAC TGCTTAGAAA ATGTGGACAA AGCTCTTCAT
    TTTCTTAAGG AGCAAAGAGT CCATCTTGAA AATATTGGTC CTCATGATAT TGTCAACGGC
    AATCATCGAT TGGTATTGGG CCTTATATGG ACCATCATAT TACGTTTCCA GATCCAAGAT
    ATTATAATTG AAATGAAAGG CAGTGAAACT CGCTCTGCAA AGGATGCTTT GCTATTATGG
    TGCCAGATGA AGACAGCAGG GTATCCAAAT GTTAATGTGA CTAATTTCAC ATCAAGCTGG
    AGGGATGGAC TGGCATTCAA TGCGTTAATC CATAAACACA GGCCTGACCT GTTTGATTTT
    GAAAGAATGA AAAAATCCAA TGCAAAGCAC AACCTTGAGC ATGCCTTTGA TACAGCTGAT
    CACAAATTGG GAATAACTCA GCTCCTTGAT CCAGAAGATG TCCTTACCGA AAACCCAGAT
    GAAAAATCCA TAATCACCTA CGTAGTAGCA TTTTATCATT ATTTTTCAAA GATGAAGGCC
    TTGGCTGTAG AAGGAAAAAG AATTGGCAAG GTGTTGGATA ATGCGATTGA AACAGATAAA
    ATGATCTATG AATATGAAAG CCTGACATCG GATTTGTTGA CATGGATTGA ACAAACCATC
    TTAGCCCTTA TAAATCGAAC ATTTGCAAAC TCTTTAACTG GGGTCCAACA GCAGCTCCAG
    GCATTCAGTG CATACAGAAC TACAGAAAAA CCACCTAAAT TTCAAGAAAA GGGTTATTTG
    GAGGTGCTCT TATTCACCAT TCAATCTAGA ATGAGAGAAA ACAAACAGAA AGTATATGTC
    CCTCAAGATG GGAAGCTTGT TTCTGACATC AACCGGGCAT GGGGTCGCCT TGAAAAAGCT
    GAGCATGACC GTGAGACAGC ACTGAGAAAT GAACTTATTC GACAAGAAAA GCTTGAGCAA
    CTGGCAAAGC GGTTCGATCG CAAGGCTGCA ATGCGAGAAA CTTGGATTTC GGAAAACCAG
    CACCTCATTG CACAGGATAA TTTTGGTTTT GATTTGCCAT CAGTGGAAGC CGCCAAGAAG
    AAACATGAAG CTATTGAGAC GGACATCTAT GCATATGAAG AACGTATACA AGCACTAGTA
    AATGTAGCTA AGGAACTGGA GAATGAGAGA TACCATGATA TCAGGCGTAT TAGTGCCAGA
    AAGGACAACA TTGTGCGCCT GTGGCAGTAT TTACTGGAGA TACTGAAGTC TAGGCGCAAT
    AGACTTAACA TGACTGTTGA ACTGCAAACC TTGTTTCAAG AGATGCTGCA CATTGTAAAC
    TGGATGGATG AAATAAAGGG AGGCCTTTTA TCCCAAGATT ATGGTAAACA TCTCCTCGAG
    GTGGAATATT TACTGCAGAA ACACAGCTTG ACAGAAGCTG ATATTGCTGC TCAGGCGGAT
    AAAGTAAAAT CATTTAGCCA GGCTGCTCTG AAGTTTGTAG GTGGAGATGG GTATCAGCCA
    TGCGATCCAC AGGTGATTAA AGATCGAGTT CAGCACCTTG AACTTTGTTA TCAGGAACTA
    GTATCACTGG CAGCACAGAG GAGGCATAAT TTGCAGCAAT CACGTTGTTT GTGGAAGTTT
    TTTGGAGACA TTGAAGAGGT GGCAAGATGG ATAAGGGAGA AAGAACAAAT CTATACTTCA
    ATGGATTTTG GAAAAGACCT AACCAGCATC TCCATTCTCC AAAGAAAACA CAGGGCTTTT
    GAGGATGAAC TCAAAGGTCT TGAAACTAAT TTGCAGCACA CTCTTAAAGA TGGAGAACAA
    ATGATTGCCA ATAAACACTA TGCGTCACCG AAAATCAAAG AACAAATTGA AAATATCAAA
    GTTCAGTGGG CTCAGCTAAA GGATTTGGCA GCCTTCCGTT TGAAAATTCT TTGTGACACT
    GAGAACTTCT TTCAGTTCCA GGTTGATGCT GATGATCTGG CTGCAAGGAT GCAGGACACC
    TATCGTATGA TGCAAAGTGA CGATGTTGGT CATGATGAAT ATTCAACCAA AATATTAGTG
    AAAAAGCAAA AGGATCTCTT AGATGACATA ATGATCAATA GAAAGGTTCT GGAGGGTCTC
    AGTGAGGTGG TTCAAAGATT CCCAGAGGAG TTCAAGACTT CACCTGAGAT TGATAGTCGA
    CTACAAAAAT TAAGAGCTCT CTTTTCTGAT TTAGCATCCC TAGCAGACTT GCGTTCCCAA
    AAACTTCAAG ATGCTCTCTC ATTTTATACC GTTCTTGGTG ACATAGATGC ATGCGAGATG
    TGGATGAATG AAAAGGAGAA ATGGCTGGAA AAGATGGAGA TTCCAGAATC ACTAGAGGAC
    ATGGGAGTTG TACAGCATAG ATTTAATACA TTAGACCAAG AAATGGGTGC TCTGGGCTCC
    CAAATTCAGG ATGTGAACAC AGCAGCCAAT GCTCTTATTG AAAGTGGACA TCCCAGTAGG
    AAAAAAGTAA AGGAAGATCT GGATCACATG AATAGCAGAT GGACAGTGTT TCAAGAGATG
    GTTTCCCAAA AGCGAAGAGC AGTTGATTCT GCCCTTGGCC TTCACAATTA TGGTGTGGAA
    TGTGATGAGA CCAAGAACTG GATAATCGAA AAAATCAAGG TTGTAGAATC TATTCAGGAT
    CTTGGAAGTG ATCTTGCGTC TGTGATGTCA ATTCAAAGGA AACTCTATGG CATTGAGAGA
    GATCTAGCCG CTATTGAGGC CAAACTCATA AATCTACAAG GAGATGCACA AAAACTTGCA
    AATGATCACC CTGATCAAGC TCAAAACATC CTTGATCGAC TGATTGATAT AAATAAGAAA
    TGGGAAGAAT TGCAGAAGGC ACTGCAAAAT CAAGAGGAGT CCTTAGGAGA AAGTAGCAAA
    GTACAAAAAT TCTTGGTGGA CTTGTTTGAT TTTGAGACAT GGCTTTACAG AGCCCAACAG
    GCAACAGCTT CAGAAGATAT ACCCACTTCA CTGCCAGAGG CCGAAAAGCT ATATGCTAAC
    CACCTTTCTC TTAAAGATGA CATTGAACGC CATGAACCCG ATTTCTGTGA TGTTGTAGAT
    ACAGGTAACA AGGTAACCTC AGGACAAACA GACCCCCAAT ATGAACAACT TGATCAAAGA
    GGCAACGATA TAAAAGAAGG CTGGAATGAT CTCCACAAGA TGTGGGACAA TCGAGAGAAA
    TCCCTTTCTC AGTGCTTGGA ATTCCAGAAG TTCCTGAAGG ATGCAAAGCA GTCTGAAGCT
    ATTTTGAGTA ATCAGGAATA CACATTTGCT CATATTGAAG TTCCCACCAC ATTACAAGCT
    TCACAAACTA GACTGAAAAA ACATGAGGAT TTCCTAGCTA CTATGGAGAA TAATGACGAG
    AAGATTGTTG GAACCATCAA TCATGGAAAG AAATTAGATG AGGATAAAAA TGTTTTTGCT
    GATAAAGCTT TGGGCAAGAT TTCCTTTATT GAAGAAAGAT ATGCCAAGAA CTTAAACAAG
    GCCAAAGAAG TTTCTATTCT GCTGAAGGAT AACTGCGACC TCCAAAATTT CCTTGAAAAC
    TGCGAAGAGC TCAATATATG GATCAATGAA AAAATGTTGA CTGCTCAAGA CACATCTTAT
    GATGGAGCTC GAAACCTGCA CAGCAAATGG CTAAAGCACC AGGCTTTTAA GGCAGAACTT
    GATTCCAACA AAGAGAGACT TGAGAATCTT GACAAGGAAG GGAAACAACT GGCACAGGAA
    AAAGAACAGT TTGCACCAAT TATTTCTGAG AAACTGAGTG AGTTGCACAG ACTCTGGGAT
    GAACTGGAAA CTACCACCCT CCAAAAAGAA CAGTCTCTTT TTGATGCCCA CCGCACAGAG
    CTTTATGAGC AAAGATATGC AGATCTAAAT AAATGGATAA AAGATATGGA GCAGCAAGTA
    AAATCTGATG ACTGTGGAAC AGACCTTACA AGTGTTAATA TTCTACTAAA TAAGCACAAA
    AGAATTGAAG ATCAGATTCA ACAAAAGACA ATAGAAATGG ATCAAGTTCT ACCACAAGCT
    TCAGCTCTGG GCAAAGAACC TGATGAGAAG GAGCCAGAAA TTAACGTACG TTTTCTGCAA
    CTTGAGCCGC TAATGCAGGA GAGAAAGAGG AAACTTCTTT CTTCAAAGCA AGTACATCAA
    CTGCACCGGG ACTTGGAAGA TGAAATTCTT TGGATTCAGG AGAGATTGCC TCTTGCAGAG
    TCACAGGATT ATGGAAATAA CCTTCAGACG GTACAAATGC TAATGAAGAA AAATCAGACT
    TTACAGAAAG AGATTGCAGG TCACAGTCCC CGGATAGAGG ATGTTCTAAT GAGAGCAAAG
    CATATAGAAC ATGATGCAGA TATTGATGAT CAGCCTATTG AAAAGAAACG GAAGTCTTTG
    GAAGAGAGCT GGGATAACTT ACAGAAACAA ATAGCAGAAA GAGAAAAACA CTTGCTGCAA
    GCTCTTGAAG CACACCATTA TTATATGGAT GCAGGCGAGG CAGAGGCTTG GATTGATGAA
    CAACAGCTTT ACCTGATTGG AGATGAACAA CCAAAGGATG AAGAAAGTGT TGTTGCAATG
    TTGAAGAAAC ATCTCAATTT ACAACATGCC GTGGAACATT ATGGGAAGAA TATTAAGGAC
    TTGGCAGACC AGGCACAAAA ACTCCTTGCA TTAGAGCATC CTGATAGTGA ACGTATAACT
    CGGCACCAAG GCAATATAGA AAAGCAGTAC GCAGCACTGA GGGACATGGC TGATGAGAAA
    AGGCAAAAGC TGGACAATAT TTATCACATG TTTCAGCTGA GGCGTGAAGT TGAAGATCTG
    GAGCAATGGA TTGCAGAAAG AGATGTGAAG GCCTCATCCC AGGAAATGGG ACAAGATTTG
    GATCATGTGT CAATCCTGAG AGACAAATTC CGTGACTTTG CGAGAGAGAC AGGAGTCATT
    GGGCAGGAGC GTATTGATAA CGTCAATATT TTAATTGAAC AACTGATTGA TGCTGGCCAT
    GCAGATGCAG CAATGATTGC AGAATGGAAA GATAACTTAA ATGAGAGCTG GGCAGACTTG
    CTGGAACTTA TAGATACCCG TGTTCAGCTG CTGTCTGCAT CCTATGAACT CCATAAATTC
    TTCTATGATG GTACAGAAAT CCTAAGCATG ATTGAGGAGA AGCACAAGGA ATTGCCAGAA
    GATTTGGGGG GAGATGTACA CACAGCACAA TCACTTCACC GAGTGCATAC TGCATTTGAA
    AGAGATATTC ATTTTATTGG GAGCCAGGTA CAACACTTCC AAGACACAGC AGCACGTTTA
    TATGCTGCCT ATGCAGGAAC ACAGGCTGTG GATATACAGA AAAAGGAGCA AGAAGTTGTT
    GAAGCCTGGA AAGGCCTTCT GGATGCCTGT GATGGGCGTC GAACAGAGTT GATTAGTGCA
    GCAGAGAAAT TTCGGTTTTT CAGCATGGTG CGTGATTTGA TGCTTTGGAT GGAGAACATG
    ATTAGACAGA TTGAGACACA GGAAAAACCA AGAGATATAT CATCTGTTGA GTACCTAATA
    AAATACCACC AAGAACTAAA GGCAGAAATC GAAGCTAGAA ATGTCACATA TACAGCATGT
    GTGGATTTGG GAAACACTCT CTTGGCTCGT AATCACCCAG CTTCTGAGGA GATAAAAGAA
    AAGTTGGTGC AGCTAGAAGA ACGGAGAGAG GAGATGATGG AGAAGTGGCA CAAGCGCTGG
    GACTGGCTTA GTATTTTACT AGAGGTTTGC CAGTTTGCAA GAGACGCCTC CATGGCAGAA
    TCATGGTTAA TATCAAAGGA GCCATACCTG TACAGTACTT ATGTGGGAAA CAGCGTTGAT
    GATGTGGAAA AGTTAATCAG AAGGCATGAA TCATTTGAGA AATCCACTTT AACATGGGAA
    GAGAGATTTT CTGCGCTAGA AAGGCTAACA ACACTGGAGC TATTAGAAGT AAGGAAATAC
    CAGGAAGCTA AAAGACATGA AATGTTAATG GCTGCAGAAT CAGAAATGAA AGAGACAGCC
    CAGAAGGAAC AACAATTCCA AACACATGAA GAAAAAGAAC ATGAAGTGAA AGCCATTATC
    CATGCTGCCC CTGACTCTCC AGTGTTAGAG GAGCTAAAAC CAAGATTGCC CAGTATAACA
    GAAGTAACTG AAGAAACTAA GGAGAAAGAA GAGGAGAAGA TCACTCAGGA AGAGTATACA
    CATACCGAAT CCATGGAACA GACTGAATAT AGCATTTTAG ATCCAGAAAC CAGACAAATC
    GAAGCAACAA CTTTACCAAC AGATTCTGGT AGCTCTAGCA GTATTCAGCA TCAGGGTTAC
    CTAAACAGGA AACATGACCT TGATGGAACA CTTAAAAAAG CAGCAAACAG GTCCTGGAAC
    AATGTCTACT GTGTGCTTAA GAACCATGAT TTGTACTTTT ACAAAGATGC CAAATGCTTT
    GCTTTGAATG AAGCTTTCCA AGGTGAAGGC CCTCTGCCCC TGCGTAATGC AAGCTGTGAA
    ATAGTGGCCG ACTATAAAAA GAAAAAACAC GTCTTTAGGC TTCGACTCGG TGATGGAAAT
    GAATGGCTAT TTCAAGCGAA AGATGAGGCT GATCTTCAAA CCTGGGTGCC GATTCTTGCA
    ACAACCATTA CAGAGGCTAC AGATCATAAA CCTAAGTCCA AAAGTCTTCC TCTGCCCTCC
    ACATCTGCAT CTGAAGGAAA GCCAGAAAAG AAAGATAAAC GATTTACATT TTTCTCCTCA
    AAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa15023
    Clone ID Related Accession (Same CDS sequence) XM_002936896.3 , XM_004917264.3
    Accession Version XM_002936896.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin beta chain, erythrocytic
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002936896.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGACCA CTGACTTTGA TAACCTGGAA ATTGAGCAGC AGTACTGCAC TATCAACTCC 
    CGCTGGGAAA CGGTAGAGGA TGACCTTGAC AATGATAACA GTTCCGCTCG AATGTTTGAA
    AGATCTAGAA TTAAAGCCCT TGCAGATGAG CGTGAAGCCG TGCAAAAGAA AACATTTACC
    AAATGGGTCA ACTCCCATCT TGCTTTTGTA AGTTGTCGCA TTTCTGATCT CTACTTGGAT
    CTGAGAGATG GAAGGATGTT AATAAAACTT TTGGAGGTGC TATCAGGAGA ACAGCTGCCA
    AGAAGAACAA AAGGGAGAAT GCGTATCCAC TGCTTAGAAA ATGTGGACAA AGCTCTTCAT
    TTTCTTAAGG AGCAAAGAGT CCATCTTGAA AATATTGGTC CTCATGATAT TGTCAACGGC
    AATCATCGAT TGGTATTGGG CCTTATATGG ACCATCATAT TACGTTTCCA GATCCAAGAT
    ATTATAATTG AAATGAAAGG CAGTGAAACT CGCTCTGCAA AGGATGCTTT GCTATTATGG
    TGCCAGATGA AGACAGCAGG GTATCCAAAT GTTAATGTGA CTAATTTCAC ATCAAGCTGG
    AGGGATGGAC TGGCATTCAA TGCGTTAATC CATAAACACA GGCCTGACCT GTTTGATTTT
    GAAAGAATGA AAAAATCCAA TGCAAAGCAC AACCTTGAGC ATGCCTTTGA TACAGCTGAT
    CACAAATTGG GAATAACTCA GCTCCTTGAT CCAGAAGATG TCCTTACCGA AAACCCAGAT
    GAAAAATCCA TAATCACCTA CGTAGTAGCA TTTTATCATT ATTTTTCAAA GATGAAGGCC
    TTGGCTGTAG AAGGAAAAAG AATTGGCAAG GTGTTGGATA ATGCGATTGA AACAGATAAA
    ATGATCTATG AATATGAAAG CCTGACATCG GATTTGTTGA CATGGATTGA ACAAACCATC
    TTAGCCCTTA TAAATCGAAC ATTTGCAAAC TCTTTAACTG GGGTCCAACA GCAGCTCCAG
    GCATTCAGTG CATACAGAAC TACAGAAAAA CCACCTAAAT TTCAAGAAAA GGGTTATTTG
    GAGGTGCTCT TATTCACCAT TCAATCTAGA ATGAGAGAAA ACAAACAGAA AGTATATGTC
    CCTCAAGATG GGAAGCTTGT TTCTGACATC AACCGGGCAT GGGGTCGCCT TGAAAAAGCT
    GAGCATGACC GTGAGACAGC ACTGAGAAAT GAACTTATTC GACAAGAAAA GCTTGAGCAA
    CTGGCAAAGC GGTTCGATCG CAAGGCTGCA ATGCGAGAAA CTTGGATTTC GGAAAACCAG
    CACCTCATTG CACAGGATAA TTTTGGTTTT GATTTGCCAT CAGTGGAAGC CGCCAAGAAG
    AAACATGAAG CTATTGAGAC GGACATCTAT GCATATGAAG AACGTATACA AGCACTAGTA
    AATGTAGCTA AGGAACTGGA GAATGAGAGA TACCATGATA TCAGGCGTAT TAGTGCCAGA
    AAGGACAACA TTGTGCGCCT GTGGCAGTAT TTACTGGAGA TACTGAAGTC TAGGCGCAAT
    AGACTTAACA TGACTGTTGA ACTGCAAACC TTGTTTCAAG AGATGCTGCA CATTGTAAAC
    TGGATGGATG AAATAAAGGG AGGCCTTTTA TCCCAAGATT ATGGTAAACA TCTCCTCGAG
    GTGGAATATT TACTGCAGAA ACACAGCTTG ACAGAAGCTG ATATTGCTGC TCAGGCGGAT
    AAAGTAAAAT CATTTAGCCA GGCTGCTCTG AAGTTTGTAG GTGGAGATGG GTATCAGCCA
    TGCGATCCAC AGGTGATTAA AGATCGAGTT CAGCACCTTG AACTTTGTTA TCAGGAACTA
    GTATCACTGG CAGCACAGAG GAGGCATAAT TTGCAGCAAT CACGTTGTTT GTGGAAGTTT
    TTTGGAGACA TTGAAGAGGT GGCAAGATGG ATAAGGGAGA AAGAACAAAT CTATACTTCA
    ATGGATTTTG GAAAAGACCT AACCAGCATC TCCATTCTCC AAAGAAAACA CAGGGCTTTT
    GAGGATGAAC TCAAAGGTCT TGAAACTAAT TTGCAGCACA CTCTTAAAGA TGGAGAACAA
    ATGATTGCCA ATAAACACTA TGCGTCACCG AAAATCAAAG AACAAATTGA AAATATCAAA
    GTTCAGTGGG CTCAGCTAAA GGATTTGGCA GCCTTCCGTT TGAAAATTCT TTGTGACACT
    GAGAACTTCT TTCAGTTCCA GGTTGATGCT GATGATCTGG CTGCACGGAT GCAGGACACC
    TATCGTATGA TGCAAAGTGA CGATGTTGGT CATGATGAAT ATTCAACCAA AATATTAGTG
    AAAAAGCAAA AGGATCTCTT AGATGACATA ATGATCAATA GAAAGGTTCT GGAGGGTCTC
    AGTGAGGTGG TTCAAAGATT CCCAGAGGAG TTCAAGACTT CACCTGAGAT TGATAGTCGA
    CTACAAAAAT TAAGAGCTCT CTTTTCTGAT TTAGCATCCC TAGCAGACTT GCGTTCCCAA
    AAACTTCAAG ATGCTCTCTC ATTTTATACC GTTCTTGGTG ACATAGATGC ATGCGAGATG
    TGGATGAATG AAAAGGAGAA ATGGCTGGAA AAGATGGAGA TTCCAGAATC ACTAGAGGAC
    ATGGGAGTTG TACAGCATAG ATTTAATACA TTAGACCAAG AAATGGGTGC TCTGGGCTCC
    CAAATTCAGG ATGTGAACAC AGCAGCCAAT GCTCTTATTG AAAGTGGACA TCCCAGTAGG
    AAAAAAGTAA AGGAAGATCT GGATCACATG AATAGCAGAT GGACAGTGTT TCAAGAGATG
    GTTTCCCAAA AGCGAAGAGC AGTTGATTCT GCCCTTGGCC TTCACAATTA TGGTGTGGAA
    TGTGATGAGA CCAAGAACTG GATAATCGAA AAAATCAAGG TTGTAGAATC TATTCAGGAT
    CTTGGAAGTG ATCTTGCGTC TGTGATGTCA ATTCAAAGGA AACTCTATGG CATTGAGAGA
    GATCTAGCCG CTATTGAGGC CAAACTCATA AATCTACAAG GAGATGCACA AAAACTTGCA
    AATGATCACC CTGATCAAGC TCAAAACATC CTTGATCGAC TGATTGATAT AAATAAGAAA
    TGGGAAGAAT TGCAGAAGGC ACTGCAAAAT CAAGAGGAGT CCTTAGGAGA AAGTAGCAAA
    GTACAAAAAT TCTTGGTGGA CTTGTTTGAT TTTGAGACAT GGCTTTACAG AGCCCAACAG
    GCAACAGCTT CAGAAGATAT ACCCACTTCA CTGCCAGAGG CCGAAAAGCT ATATGCTAAC
    CACCTTTCTC TTAAAGATGA CATTGAACGC CATGAACCCG ATTTCTGTGA TGTTGTAGAT
    ACAGGTAACA AGGTAACCTC AGGACAAACA GACCCCCAAT ATGAACAACT TGATCAAAGA
    GGCAACGATA TAAAAGAAGG CTGGAATGAT CTCCACAAGA TGTGGGACAA TCGAGAGAAA
    TCCCTTTCTC AGTGCTTGGA ATTCCAGAAG TTCCTGAAGG ATGCAAAGCA GTCTGAAGCT
    ATTTTGAGTA ATCAGGAATA CACATTTGCT CATATTGAAG TTCCCACCAC ATTACAAGCT
    TCACAAACTA GACTGAAAAA ACATGAGGAT TTCCTAGCTA CTATGGAGAA TAATGACGAG
    AAGATTGTTG GAACCATCAA TCATGGAAAG AAATTAGATG AGGATAAAAA TGTTTTTGCT
    GATAAAGCTT TGGGCAAGAT TTCCTTTATT GAAGAAAGAT ATGCCAAGAA CTTAAACAAG
    GCCAAAGAAG TTTCTATTCT GCTGAAGGAT AACTGCGACC TCCAAAATTT CCTTGAAAAC
    TGCGAAGAGC TCAATATATG GATCAATGAA AAAATGTTGA CTGCTCAAGA CACATCTTAT
    GATGGAGCTC GAAACCTGCA CAGCAAATGG CTAAAGCACC AGGCTTTTAA GGCAGAACTT
    GATTCCAACA AAGAGAGACT TGAGAATCTT GACAAGGAAG GGAAACAACT GGCACAGGAA
    AAAGAACAGT TTGCACCAAT TATTTCTGAG AAACTGAGTG AGTTGCACAG ACTCTGGGAT
    GAACTGGAAA CTACCACCCT CCAAAAAGAA CAGTCTCTTT TTGATGCCCA CCGCACAGAG
    CTTTATGAGC AAAGATATGC AGATCTAAAT AAATGGATAA AAGATATGGA GCAGCAAGTA
    AAATCTGATG ACTGTGGAAC AGACCTTACA AGTGTTAATA TTCTACTAAA TAAGCACAAA
    AGAATTGAAG ATCAGATTCA ACAAAAGACA ATAGAAATGG ATCAAGTTCT ACCACAAGCT
    TCAGCTCTGG GCAAAGAACC TGATGAGAAG GAGCCAGAAA TTAACGTACG TTTTCTGCAA
    CTTGAGCCGC TAATGCAGGA GAGAAAGAGG AAACTTCTTT CTTCAAAGCA AGTACATCAA
    CTGCACCGGG ACTTGGAAGA TGAAATTCTT TGGATTCAGG AGAGATTGCC TCTTGCAGAG
    TCACAGGATT ATGGAAATAA CCTTCAGACG GTACAAATGC TAATGAAGAA AAATCAGACT
    TTACAGAAAG AGATTGCAGG TCACAGTCCC CGGATAGAGG ATGTTCTAAT GAGAGCAAAG
    CATATAGAAC ATGATGCAGA TATTGATGAT CAGCCTATTG AAAAGAAACG GAAGTCTTTG
    GAAGAGAGCT GGGATAACTT ACAGAAACAA ATAGCAGAAA GAGAAAAACA CTTGCTGCAA
    GCTCTTGAAG CACACCATTA TTATATGGAT GCAGGCGAGG CAGAGGCTTG GATTGATGAA
    CAACAGCTTT ACCTGATTGG AGATGAACAA CCAAAGGATG AAGAAAGTGT TGTTGCAATG
    TTGAAGAAAC ATCTCAATTT ACAACATGCC GTGGAACATT ATGGGAAGAA TATTAAGGAC
    TTGGCAGACC AGGCACAAAA ACTCCTTGCA TTAGAGCATC CTGATAGTGA ACGTATAACT
    CGGCACCAAG GCAATATAGA AAAGCAGTAC GCAGCACTGA GGGACATGGC TGATGAGAAA
    AGGCAAAAGC TGGACAATAT TTATCACATG TTTCAGCTGA GGCGTGAAGT TGAAGATCTG
    GAGCAATGGA TTGCAGAAAG AGATGTGAAG GCCTCATCCC AGGAAATGGG ACAAGATTTG
    GATCATGTGT CAATCCTGAG AGACAAATTC CGTGACTTTG CGAGAGAGAC AGGAGTCATT
    GGGCAGGAGC GTATTGATAA CGTCAATATT TTAATTGAAC AACTGATTGA TGCTGGCCAT
    GCAGATGCAG CAATGATTGC AGAATGGAAA GATAACTTAA ATGAGAGCTG GGCAGACTTG
    CTGGAACTTA TAGATACCCG TGTTCAGCTG CTGTCTGCAT CCTATGAACT CCATAAATTC
    TTCTATGATG GTACAGAAAT CCTAAGCATG ATTGAGGAGA AGCACAAGGA ATTGCCAGAA
    GATTTGGGGG GAGATGTACA CACAGCACAA TCACTTCACC GAGTGCATAC TGCATTTGAA
    AGAGATATTC ATTTTATTGG GAGCCAGGTA CAACACTTCC AAGACACAGC AGCACGTTTA
    TATGCTGCCT ATGCAGGAAC ACAGGCTGTG GATATACAGA AAAAGGAGCA AGAAGTTGTT
    GAAGCCTGGA AAGGCCTTCT GGATGCCTGT GATGGGCGTC GAACAGAGTT GATTAGTGCA
    GCAGAGAAAT TTCGGTTTTT CAGCATGGTG CGTGATTTGA TGCTTTGGAT GGAGAACATG
    ATTAGACAGA TTGAGACACA GGAAAAACCA AGAGATATAT CATCTGTTGA GTACCTAATA
    AAATACCACC AAGAACTAAA GGCAGAAATC GAAGCTAGAA ATGTCACATA TACAGCATGT
    GTGGATTTGG GAAACACTCT CTTGGCTCGT AATCACCCAG CTTCTGAGGA GATAAAAGAA
    AAGTTGGTGC AGCTAGAAGA ACGGAGAGAG GAGATGATGG AGAAGTGGCA CAAGCGCTGG
    GACTGGCTTA GTATTTTACT AGAGGTTTGC CAGTTTGCAA GAGACGCCTC CATGGCAGAA
    TCATGGTTAA TATCAAAGGA GCCATACCTG TACAGTACTT ATGTGGGAAA CAGCGTTGAT
    GATGTGGAAA AGTTAATCAG AAGGCATGAA TCATTTGAGA AATCCACTTT AACATGGGAA
    GAGAGATTTT CTGCGCTAGA AAGGCTAACA ACACTGGAGC TATTAGAAGT AAGGAAATAC
    CAGGAAGCTA AAAGACATGA AATGTTAATG GCTGCAGAAT CAGAAATGAA AGAGACAGCC
    CAGAAGGAAC AACAATTCCA AACACATGAA GAAAAAGAAC ATGAAGTGAA AGCCATTATC
    CATGCTGCCC CTGACTCTCC AGTGTTAGAG GAGCTAAAAC CAAGATTGCC CAGTATAACA
    GAAGTAACTG AAGAAACTAA GGAGAAAGAA GAGGAGAAGA TCACTCAGGA AGAGTATACA
    CATACCGAAT CCATGGAACA GACTGAATAT AGCATTTTAG ATCCAGAAAC CAGACAAATC
    GAAGCAACAA CTTTACCAAC AGATTCTGGT AGCTCTAGCA GTATTCAGCA TCAGGGTTAC
    CTAAACAGGA AACATGACCT TGATGGAACA CTTAAAAAAG CAGCAAACAG GTCCTGGAAC
    AATGTCTACT GTGTGCTTAA GAACCATGAT TTGTACTTTT ACAAAGATGC CAAATGCTTT
    GCTTTGAATG AAGCTTTCCA AGGTGAAGGC CCTCTGCCCC TGCGTAATGC AAGCTGTGAA
    ATAGTGGCCG ACTATAAAAA GAAAAAACAC GTCTTTAGGC TTCGACTCGG TGATGGAAAT
    GAATGGCTAT TTCAAGCGAA AGATGAGGCT GATCTTCAAA CCTGGGTGCC GATTCTTGCA
    ACAACCATTA CAGAGGCTAC AGATCATAAA CCTAAGTCCA AAAGTCTTCC TCTGCCCTCC
    ACATCTGCAT CTGAAGGAAA GCCAGAAAAG AAAGATAAAC GATTTACATT TTTCTCCTCA
    AAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002936942.2
    CDS166..7194
    Translation

    Target ORF information:

    RefSeq Version XM_002936896.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, beta, erythrocytic (sptb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002936896.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGACCA CTGACTTTGA TAACCTGGAA ATTGAGCAGC AGTACTGCAC TATCAACTCC 
    CGCTGGGAAA CGGTAGAGGA TGACCTTGAC AATGATAACA GTTCCGCTCG AATGTTTGAA
    AGATCTAGAA TTAAAGCCCT TGCAGATGAG CGTGAAGCCG TGCAAAAGAA AACATTTACC
    AAATGGGTCA ACTCCCATCT TGCTTTTGTA AGTTGTCGCA TTTCTGATCT CTACTTGGAT
    CTGAGAGATG GAAGGATGTT AATAAAACTT TTGGAGGTGC TATCAGGAGA ACAGCTGCCA
    AGAAGAACAA AAGGGAGAAT GCGTATCCAC TGCTTAGAAA ATGTGGACAA AGCTCTTCAT
    TTTCTTAAGG AGCAAAGAGT CCATCTTGAA AATATTGGTC CTCATGATAT TGTCAACGGC
    AATCATCGAT TGGTATTGGG CCTTATATGG ACCATCATAT TACGTTTCCA GATCCAAGAT
    ATTATAATTG AAATGAAAGG CAGTGAAACT CGCTCTGCAA AGGATGCTTT GCTATTATGG
    TGCCAGATGA AGACAGCAGG GTATCCAAAT GTTAATGTGA CTAATTTCAC ATCAAGCTGG
    AGGGATGGAC TGGCATTCAA TGCGTTAATC CATAAACACA GGCCTGACCT GTTTGATTTT
    GAAAGAATGA AAAAATCCAA TGCAAAGCAC AACCTTGAGC ATGCCTTTGA TACAGCTGAT
    CACAAATTGG GAATAACTCA GCTCCTTGAT CCAGAAGATG TCCTTACCGA AAACCCAGAT
    GAAAAATCCA TAATCACCTA CGTAGTAGCA TTTTATCATT ATTTTTCAAA GATGAAGGCC
    TTGGCTGTAG AAGGAAAAAG AATTGGCAAG GTGTTGGATA ATGCGATTGA AACAGATAAA
    ATGATCTATG AATATGAAAG CCTGACATCG GATTTGTTGA CATGGATTGA ACAAACCATC
    TTAGCCCTTA TAAATCGAAC ATTTGCAAAC TCTTTAACTG GGGTCCAACA GCAGCTCCAG
    GCATTCAGTG CATACAGAAC TACAGAAAAA CCACCTAAAT TTCAAGAAAA GGGTTATTTG
    GAGGTGCTCT TATTCACCAT TCAATCTAGA ATGAGAGAAA ACAAACAGAA AGTATATGTC
    CCTCAAGATG GGAAGCTTGT TTCTGACATC AACCGGGCAT GGGGTCGCCT TGAAAAAGCT
    GAGCATGACC GTGAGACAGC ACTGAGAAAT GAACTTATTC GACAAGAAAA GCTTGAGCAA
    CTGGCAAAGC GGTTCGATCG CAAGGCTGCA ATGCGAGAAA CTTGGATTTC GGAAAACCAG
    CACCTCATTG CACAGGATAA TTTTGGTTTT GATTTGCCAT CAGTGGAAGC CGCCAAGAAG
    AAACATGAAG CTATTGAGAC GGACATCTAT GCATATGAAG AACGTATACA AGCACTAGTA
    AATGTAGCTA AGGAACTGGA GAATGAGAGA TACCATGATA TCAGGCGTAT TAGTGCCAGA
    AAGGACAACA TTGTGCGCCT GTGGCAGTAT TTACTGGAGA TACTGAAGTC TAGGCGCAAT
    AGACTTAACA TGACTGTTGA ACTGCAAACC TTGTTTCAAG AGATGCTGCA CATTGTAAAC
    TGGATGGATG AAATAAAGGG AGGCCTTTTA TCCCAAGATT ATGGTAAACA TCTCCTCGAG
    GTGGAATATT TACTGCAGAA ACACAGCTTG ACAGAAGCTG ATATTGCTGC TCAGGCGGAT
    AAAGTAAAAT CATTTAGCCA GGCTGCTCTG AAGTTTGTAG GTGGAGATGG GTATCAGCCA
    TGCGATCCAC AGGTGATTAA AGATCGAGTT CAGCACCTTG AACTTTGTTA TCAGGAACTA
    GTATCACTGG CAGCACAGAG GAGGCATAAT TTGCAGCAAT CACGTTGTTT GTGGAAGTTT
    TTTGGAGACA TTGAAGAGGT GGCAAGATGG ATAAGGGAGA AAGAACAAAT CTATACTTCA
    ATGGATTTTG GAAAAGACCT AACCAGCATC TCCATTCTCC AAAGAAAACA CAGGGCTTTT
    GAGGATGAAC TCAAAGGTCT TGAAACTAAT TTGCAGCACA CTCTTAAAGA TGGAGAACAA
    ATGATTGCCA ATAAACACTA TGCGTCACCG AAAATCAAAG AACAAATTGA AAATATCAAA
    GTTCAGTGGG CTCAGCTAAA GGATTTGGCA GCCTTCCGTT TGAAAATTCT TTGTGACACT
    GAGAACTTCT TTCAGTTCCA GGTTGATGCT GATGATCTGG CTGCACGGAT GCAGGACACC
    TATCGTATGA TGCAAAGTGA CGATGTTGGT CATGATGAAT ATTCAACCAA AATATTAGTG
    AAAAAGCAAA AGGATCTCTT AGATGACATA ATGATCAATA GAAAGGTTCT GGAGGGTCTC
    AGTGAGGTGG TTCAAAGATT CCCAGAGGAG TTCAAGACTT CACCTGAGAT TGATAGTCGA
    CTACAAAAAT TAAGAGCTCT CTTTTCTGAT TTAGCATCCC TAGCAGACTT GCGTTCCCAA
    AAACTTCAAG ATGCTCTCTC ATTTTATACC GTTCTTGGTG ACATAGATGC ATGCGAGATG
    TGGATGAATG AAAAGGAGAA ATGGCTGGAA AAGATGGAGA TTCCAGAATC ACTAGAGGAC
    ATGGGAGTTG TACAGCATAG ATTTAATACA TTAGACCAAG AAATGGGTGC TCTGGGCTCC
    CAAATTCAGG ATGTGAACAC AGCAGCCAAT GCTCTTATTG AAAGTGGACA TCCCAGTAGG
    AAAAAAGTAA AGGAAGATCT GGATCACATG AATAGCAGAT GGACAGTGTT TCAAGAGATG
    GTTTCCCAAA AGCGAAGAGC AGTTGATTCT GCCCTTGGCC TTCACAATTA TGGTGTGGAA
    TGTGATGAGA CCAAGAACTG GATAATCGAA AAAATCAAGG TTGTAGAATC TATTCAGGAT
    CTTGGAAGTG ATCTTGCGTC TGTGATGTCA ATTCAAAGGA AACTCTATGG CATTGAGAGA
    GATCTAGCCG CTATTGAGGC CAAACTCATA AATCTACAAG GAGATGCACA AAAACTTGCA
    AATGATCACC CTGATCAAGC TCAAAACATC CTTGATCGAC TGATTGATAT AAATAAGAAA
    TGGGAAGAAT TGCAGAAGGC ACTGCAAAAT CAAGAGGAGT CCTTAGGAGA AAGTAGCAAA
    GTACAAAAAT TCTTGGTGGA CTTGTTTGAT TTTGAGACAT GGCTTTACAG AGCCCAACAG
    GCAACAGCTT CAGAAGATAT ACCCACTTCA CTGCCAGAGG CCGAAAAGCT ATATGCTAAC
    CACCTTTCTC TTAAAGATGA CATTGAACGC CATGAACCCG ATTTCTGTGA TGTTGTAGAT
    ACAGGTAACA AGGTAACCTC AGGACAAACA GACCCCCAAT ATGAACAACT TGATCAAAGA
    GGCAACGATA TAAAAGAAGG CTGGAATGAT CTCCACAAGA TGTGGGACAA TCGAGAGAAA
    TCCCTTTCTC AGTGCTTGGA ATTCCAGAAG TTCCTGAAGG ATGCAAAGCA GTCTGAAGCT
    ATTTTGAGTA ATCAGGAATA CACATTTGCT CATATTGAAG TTCCCACCAC ATTACAAGCT
    TCACAAACTA GACTGAAAAA ACATGAGGAT TTCCTAGCTA CTATGGAGAA TAATGACGAG
    AAGATTGTTG GAACCATCAA TCATGGAAAG AAATTAGATG AGGATAAAAA TGTTTTTGCT
    GATAAAGCTT TGGGCAAGAT TTCCTTTATT GAAGAAAGAT ATGCCAAGAA CTTAAACAAG
    GCCAAAGAAG TTTCTATTCT GCTGAAGGAT AACTGCGACC TCCAAAATTT CCTTGAAAAC
    TGCGAAGAGC TCAATATATG GATCAATGAA AAAATGTTGA CTGCTCAAGA CACATCTTAT
    GATGGAGCTC GAAACCTGCA CAGCAAATGG CTAAAGCACC AGGCTTTTAA GGCAGAACTT
    GATTCCAACA AAGAGAGACT TGAGAATCTT GACAAGGAAG GGAAACAACT GGCACAGGAA
    AAAGAACAGT TTGCACCAAT TATTTCTGAG AAACTGAGTG AGTTGCACAG ACTCTGGGAT
    GAACTGGAAA CTACCACCCT CCAAAAAGAA CAGTCTCTTT TTGATGCCCA CCGCACAGAG
    CTTTATGAGC AAAGATATGC AGATCTAAAT AAATGGATAA AAGATATGGA GCAGCAAGTA
    AAATCTGATG ACTGTGGAAC AGACCTTACA AGTGTTAATA TTCTACTAAA TAAGCACAAA
    AGAATTGAAG ATCAGATTCA ACAAAAGACA ATAGAAATGG ATCAAGTTCT ACCACAAGCT
    TCAGCTCTGG GCAAAGAACC TGATGAGAAG GAGCCAGAAA TTAACGTACG TTTTCTGCAA
    CTTGAGCCGC TAATGCAGGA GAGAAAGAGG AAACTTCTTT CTTCAAAGCA AGTACATCAA
    CTGCACCGGG ACTTGGAAGA TGAAATTCTT TGGATTCAGG AGAGATTGCC TCTTGCAGAG
    TCACAGGATT ATGGAAATAA CCTTCAGACG GTACAAATGC TAATGAAGAA AAATCAGACT
    TTACAGAAAG AGATTGCAGG TCACAGTCCC CGGATAGAGG ATGTTCTAAT GAGAGCAAAG
    CATATAGAAC ATGATGCAGA TATTGATGAT CAGCCTATTG AAAAGAAACG GAAGTCTTTG
    GAAGAGAGCT GGGATAACTT ACAGAAACAA ATAGCAGAAA GAGAAAAACA CTTGCTGCAA
    GCTCTTGAAG CACACCATTA TTATATGGAT GCAGGCGAGG CAGAGGCTTG GATTGATGAA
    CAACAGCTTT ACCTGATTGG AGATGAACAA CCAAAGGATG AAGAAAGTGT TGTTGCAATG
    TTGAAGAAAC ATCTCAATTT ACAACATGCC GTGGAACATT ATGGGAAGAA TATTAAGGAC
    TTGGCAGACC AGGCACAAAA ACTCCTTGCA TTAGAGCATC CTGATAGTGA ACGTATAACT
    CGGCACCAAG GCAATATAGA AAAGCAGTAC GCAGCACTGA GGGACATGGC TGATGAGAAA
    AGGCAAAAGC TGGACAATAT TTATCACATG TTTCAGCTGA GGCGTGAAGT TGAAGATCTG
    GAGCAATGGA TTGCAGAAAG AGATGTGAAG GCCTCATCCC AGGAAATGGG ACAAGATTTG
    GATCATGTGT CAATCCTGAG AGACAAATTC CGTGACTTTG CGAGAGAGAC AGGAGTCATT
    GGGCAGGAGC GTATTGATAA CGTCAATATT TTAATTGAAC AACTGATTGA TGCTGGCCAT
    GCAGATGCAG CAATGATTGC AGAATGGAAA GATAACTTAA ATGAGAGCTG GGCAGACTTG
    CTGGAACTTA TAGATACCCG TGTTCAGCTG CTGTCTGCAT CCTATGAACT CCATAAATTC
    TTCTATGATG GTACAGAAAT CCTAAGCATG ATTGAGGAGA AGCACAAGGA ATTGCCAGAA
    GATTTGGGGG GAGATGTACA CACAGCACAA TCACTTCACC GAGTGCATAC TGCATTTGAA
    AGAGATATTC ATTTTATTGG GAGCCAGGTA CAACACTTCC AAGACACAGC AGCACGTTTA
    TATGCTGCCT ATGCAGGAAC ACAGGCTGTG GATATACAGA AAAAGGAGCA AGAAGTTGTT
    GAAGCCTGGA AAGGCCTTCT GGATGCCTGT GATGGGCGTC GAACAGAGTT GATTAGTGCA
    GCAGAGAAAT TTCGGTTTTT CAGCATGGTG CGTGATTTGA TGCTTTGGAT GGAGAACATG
    ATTAGACAGA TTGAGACACA GGAAAAACCA AGAGATATAT CATCTGTTGA GTACCTAATA
    AAATACCACC AAGAACTAAA GGCAGAAATC GAAGCTAGAA ATGTCACATA TACAGCATGT
    GTGGATTTGG GAAACACTCT CTTGGCTCGT AATCACCCAG CTTCTGAGGA GATAAAAGAA
    AAGTTGGTGC AGCTAGAAGA ACGGAGAGAG GAGATGATGG AGAAGTGGCA CAAGCGCTGG
    GACTGGCTTA GTATTTTACT AGAGGTTTGC CAGTTTGCAA GAGACGCCTC CATGGCAGAA
    TCATGGTTAA TATCAAAGGA GCCATACCTG TACAGTACTT ATGTGGGAAA CAGCGTTGAT
    GATGTGGAAA AGTTAATCAG AAGGCATGAA TCATTTGAGA AATCCACTTT AACATGGGAA
    GAGAGATTTT CTGCGCTAGA AAGGCTAACA ACACTGGAGC TATTAGAAGT AAGGAAATAC
    CAGGAAGCTA AAAGACATGA AATGTTAATG GCTGCAGAAT CAGAAATGAA AGAGACAGCC
    CAGAAGGAAC AACAATTCCA AACACATGAA GAAAAAGAAC ATGAAGTGAA AGCCATTATC
    CATGCTGCCC CTGACTCTCC AGTGTTAGAG GAGCTAAAAC CAAGATTGCC CAGTATAACA
    GAAGTAACTG AAGAAACTAA GGAGAAAGAA GAGGAGAAGA TCACTCAGGA AGAGTATACA
    CATACCGAAT CCATGGAACA GACTGAATAT AGCATTTTAG ATCCAGAAAC CAGACAAATC
    GAAGCAACAA CTTTACCAAC AGATTCTGGT AGCTCTAGCA GTATTCAGCA TCAGGGTTAC
    CTAAACAGGA AACATGACCT TGATGGAACA CTTAAAAAAG CAGCAAACAG GTCCTGGAAC
    AATGTCTACT GTGTGCTTAA GAACCATGAT TTGTACTTTT ACAAAGATGC CAAATGCTTT
    GCTTTGAATG AAGCTTTCCA AGGTGAAGGC CCTCTGCCCC TGCGTAATGC AAGCTGTGAA
    ATAGTGGCCG ACTATAAAAA GAAAAAACAC GTCTTTAGGC TTCGACTCGG TGATGGAAAT
    GAATGGCTAT TTCAAGCGAA AGATGAGGCT GATCTTCAAA CCTGGGTGCC GATTCTTGCA
    ACAACCATTA CAGAGGCTAC AGATCATAAA CCTAAGTCCA AAAGTCTTCC TCTGCCCTCC
    ACATCTGCAT CTGAAGGAAA GCCAGAAAAG AAAGATAAAC GATTTACATT TTTCTCCTCA
    AAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa15023
    Clone ID Related Accession (Same CDS sequence) XM_002936896.3 , XM_004917264.3
    Accession Version XM_004917264.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin beta chain, erythrocytic
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917264.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    481
    541
    601
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    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2581
    2641
    2701
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGACCA CTGACTTTGA TAACCTGGAA ATTGAGCAGC AGTACTGCAC TATCAACTCC 
    CGCTGGGAAA CGGTAGAGGA TGACCTTGAC AATGATAACA GTTCCGCTCG AATGTTTGAA
    AGATCTAGAA TTAAAGCCCT TGCAGATGAG CGTGAAGCCG TGCAAAAGAA AACATTTACC
    AAATGGGTCA ACTCCCATCT TGCTTTTGTA AGTTGTCGCA TTTCTGATCT CTACTTGGAT
    CTGAGAGATG GAAGGATGTT AATAAAACTT TTGGAGGTGC TATCAGGAGA ACAGCTGCCA
    AGAAGAACAA AAGGGAGAAT GCGTATCCAC TGCTTAGAAA ATGTGGACAA AGCTCTTCAT
    TTTCTTAAGG AGCAAAGAGT CCATCTTGAA AATATTGGTC CTCATGATAT TGTCAACGGC
    AATCATCGAT TGGTATTGGG CCTTATATGG ACCATCATAT TACGTTTCCA GATCCAAGAT
    ATTATAATTG AAATGAAAGG CAGTGAAACT CGCTCTGCAA AGGATGCTTT GCTATTATGG
    TGCCAGATGA AGACAGCAGG GTATCCAAAT GTTAATGTGA CTAATTTCAC ATCAAGCTGG
    AGGGATGGAC TGGCATTCAA TGCGTTAATC CATAAACACA GGCCTGACCT GTTTGATTTT
    GAAAGAATGA AAAAATCCAA TGCAAAGCAC AACCTTGAGC ATGCCTTTGA TACAGCTGAT
    CACAAATTGG GAATAACTCA GCTCCTTGAT CCAGAAGATG TCCTTACCGA AAACCCAGAT
    GAAAAATCCA TAATCACCTA CGTAGTAGCA TTTTATCATT ATTTTTCAAA GATGAAGGCC
    TTGGCTGTAG AAGGAAAAAG AATTGGCAAG GTGTTGGATA ATGCGATTGA AACAGATAAA
    ATGATCTATG AATATGAAAG CCTGACATCG GATTTGTTGA CATGGATTGA ACAAACCATC
    TTAGCCCTTA TAAATCGAAC ATTTGCAAAC TCTTTAACTG GGGTCCAACA GCAGCTCCAG
    GCATTCAGTG CATACAGAAC TACAGAAAAA CCACCTAAAT TTCAAGAAAA GGGTTATTTG
    GAGGTGCTCT TATTCACCAT TCAATCTAGA ATGAGAGAAA ACAAACAGAA AGTATATGTC
    CCTCAAGATG GGAAGCTTGT TTCTGACATC AACCGGGCAT GGGGTCGCCT TGAAAAAGCT
    GAGCATGACC GTGAGACAGC ACTGAGAAAT GAACTTATTC GACAAGAAAA GCTTGAGCAA
    CTGGCAAAGC GGTTCGATCG CAAGGCTGCA ATGCGAGAAA CTTGGATTTC GGAAAACCAG
    CACCTCATTG CACAGGATAA TTTTGGTTTT GATTTGCCAT CAGTGGAAGC CGCCAAGAAG
    AAACATGAAG CTATTGAGAC GGACATCTAT GCATATGAAG AACGTATACA AGCACTAGTA
    AATGTAGCTA AGGAACTGGA GAATGAGAGA TACCATGATA TCAGGCGTAT TAGTGCCAGA
    AAGGACAACA TTGTGCGCCT GTGGCAGTAT TTACTGGAGA TACTGAAGTC TAGGCGCAAT
    AGACTTAACA TGACTGTTGA ACTGCAAACC TTGTTTCAAG AGATGCTGCA CATTGTAAAC
    TGGATGGATG AAATAAAGGG AGGCCTTTTA TCCCAAGATT ATGGTAAACA TCTCCTCGAG
    GTGGAATATT TACTGCAGAA ACACAGCTTG ACAGAAGCTG ATATTGCTGC TCAGGCGGAT
    AAAGTAAAAT CATTTAGCCA GGCTGCTCTG AAGTTTGTAG GTGGAGATGG GTATCAGCCA
    TGCGATCCAC AGGTGATTAA AGATCGAGTT CAGCACCTTG AACTTTGTTA TCAGGAACTA
    GTATCACTGG CAGCACAGAG GAGGCATAAT TTGCAGCAAT CACGTTGTTT GTGGAAGTTT
    TTTGGAGACA TTGAAGAGGT GGCAAGATGG ATAAGGGAGA AAGAACAAAT CTATACTTCA
    ATGGATTTTG GAAAAGACCT AACCAGCATC TCCATTCTCC AAAGAAAACA CAGGGCTTTT
    GAGGATGAAC TCAAAGGTCT TGAAACTAAT TTGCAGCACA CTCTTAAAGA TGGAGAACAA
    ATGATTGCCA ATAAACACTA TGCGTCACCG AAAATCAAAG AACAAATTGA AAATATCAAA
    GTTCAGTGGG CTCAGCTAAA GGATTTGGCA GCCTTCCGTT TGAAAATTCT TTGTGACACT
    GAGAACTTCT TTCAGTTCCA GGTTGATGCT GATGATCTGG CTGCACGGAT GCAGGACACC
    TATCGTATGA TGCAAAGTGA CGATGTTGGT CATGATGAAT ATTCAACCAA AATATTAGTG
    AAAAAGCAAA AGGATCTCTT AGATGACATA ATGATCAATA GAAAGGTTCT GGAGGGTCTC
    AGTGAGGTGG TTCAAAGATT CCCAGAGGAG TTCAAGACTT CACCTGAGAT TGATAGTCGA
    CTACAAAAAT TAAGAGCTCT CTTTTCTGAT TTAGCATCCC TAGCAGACTT GCGTTCCCAA
    AAACTTCAAG ATGCTCTCTC ATTTTATACC GTTCTTGGTG ACATAGATGC ATGCGAGATG
    TGGATGAATG AAAAGGAGAA ATGGCTGGAA AAGATGGAGA TTCCAGAATC ACTAGAGGAC
    ATGGGAGTTG TACAGCATAG ATTTAATACA TTAGACCAAG AAATGGGTGC TCTGGGCTCC
    CAAATTCAGG ATGTGAACAC AGCAGCCAAT GCTCTTATTG AAAGTGGACA TCCCAGTAGG
    AAAAAAGTAA AGGAAGATCT GGATCACATG AATAGCAGAT GGACAGTGTT TCAAGAGATG
    GTTTCCCAAA AGCGAAGAGC AGTTGATTCT GCCCTTGGCC TTCACAATTA TGGTGTGGAA
    TGTGATGAGA CCAAGAACTG GATAATCGAA AAAATCAAGG TTGTAGAATC TATTCAGGAT
    CTTGGAAGTG ATCTTGCGTC TGTGATGTCA ATTCAAAGGA AACTCTATGG CATTGAGAGA
    GATCTAGCCG CTATTGAGGC CAAACTCATA AATCTACAAG GAGATGCACA AAAACTTGCA
    AATGATCACC CTGATCAAGC TCAAAACATC CTTGATCGAC TGATTGATAT AAATAAGAAA
    TGGGAAGAAT TGCAGAAGGC ACTGCAAAAT CAAGAGGAGT CCTTAGGAGA AAGTAGCAAA
    GTACAAAAAT TCTTGGTGGA CTTGTTTGAT TTTGAGACAT GGCTTTACAG AGCCCAACAG
    GCAACAGCTT CAGAAGATAT ACCCACTTCA CTGCCAGAGG CCGAAAAGCT ATATGCTAAC
    CACCTTTCTC TTAAAGATGA CATTGAACGC CATGAACCCG ATTTCTGTGA TGTTGTAGAT
    ACAGGTAACA AGGTAACCTC AGGACAAACA GACCCCCAAT ATGAACAACT TGATCAAAGA
    GGCAACGATA TAAAAGAAGG CTGGAATGAT CTCCACAAGA TGTGGGACAA TCGAGAGAAA
    TCCCTTTCTC AGTGCTTGGA ATTCCAGAAG TTCCTGAAGG ATGCAAAGCA GTCTGAAGCT
    ATTTTGAGTA ATCAGGAATA CACATTTGCT CATATTGAAG TTCCCACCAC ATTACAAGCT
    TCACAAACTA GACTGAAAAA ACATGAGGAT TTCCTAGCTA CTATGGAGAA TAATGACGAG
    AAGATTGTTG GAACCATCAA TCATGGAAAG AAATTAGATG AGGATAAAAA TGTTTTTGCT
    GATAAAGCTT TGGGCAAGAT TTCCTTTATT GAAGAAAGAT ATGCCAAGAA CTTAAACAAG
    GCCAAAGAAG TTTCTATTCT GCTGAAGGAT AACTGCGACC TCCAAAATTT CCTTGAAAAC
    TGCGAAGAGC TCAATATATG GATCAATGAA AAAATGTTGA CTGCTCAAGA CACATCTTAT
    GATGGAGCTC GAAACCTGCA CAGCAAATGG CTAAAGCACC AGGCTTTTAA GGCAGAACTT
    GATTCCAACA AAGAGAGACT TGAGAATCTT GACAAGGAAG GGAAACAACT GGCACAGGAA
    AAAGAACAGT TTGCACCAAT TATTTCTGAG AAACTGAGTG AGTTGCACAG ACTCTGGGAT
    GAACTGGAAA CTACCACCCT CCAAAAAGAA CAGTCTCTTT TTGATGCCCA CCGCACAGAG
    CTTTATGAGC AAAGATATGC AGATCTAAAT AAATGGATAA AAGATATGGA GCAGCAAGTA
    AAATCTGATG ACTGTGGAAC AGACCTTACA AGTGTTAATA TTCTACTAAA TAAGCACAAA
    AGAATTGAAG ATCAGATTCA ACAAAAGACA ATAGAAATGG ATCAAGTTCT ACCACAAGCT
    TCAGCTCTGG GCAAAGAACC TGATGAGAAG GAGCCAGAAA TTAACGTACG TTTTCTGCAA
    CTTGAGCCGC TAATGCAGGA GAGAAAGAGG AAACTTCTTT CTTCAAAGCA AGTACATCAA
    CTGCACCGGG ACTTGGAAGA TGAAATTCTT TGGATTCAGG AGAGATTGCC TCTTGCAGAG
    TCACAGGATT ATGGAAATAA CCTTCAGACG GTACAAATGC TAATGAAGAA AAATCAGACT
    TTACAGAAAG AGATTGCAGG TCACAGTCCC CGGATAGAGG ATGTTCTAAT GAGAGCAAAG
    CATATAGAAC ATGATGCAGA TATTGATGAT CAGCCTATTG AAAAGAAACG GAAGTCTTTG
    GAAGAGAGCT GGGATAACTT ACAGAAACAA ATAGCAGAAA GAGAAAAACA CTTGCTGCAA
    GCTCTTGAAG CACACCATTA TTATATGGAT GCAGGCGAGG CAGAGGCTTG GATTGATGAA
    CAACAGCTTT ACCTGATTGG AGATGAACAA CCAAAGGATG AAGAAAGTGT TGTTGCAATG
    TTGAAGAAAC ATCTCAATTT ACAACATGCC GTGGAACATT ATGGGAAGAA TATTAAGGAC
    TTGGCAGACC AGGCACAAAA ACTCCTTGCA TTAGAGCATC CTGATAGTGA ACGTATAACT
    CGGCACCAAG GCAATATAGA AAAGCAGTAC GCAGCACTGA GGGACATGGC TGATGAGAAA
    AGGCAAAAGC TGGACAATAT TTATCACATG TTTCAGCTGA GGCGTGAAGT TGAAGATCTG
    GAGCAATGGA TTGCAGAAAG AGATGTGAAG GCCTCATCCC AGGAAATGGG ACAAGATTTG
    GATCATGTGT CAATCCTGAG AGACAAATTC CGTGACTTTG CGAGAGAGAC AGGAGTCATT
    GGGCAGGAGC GTATTGATAA CGTCAATATT TTAATTGAAC AACTGATTGA TGCTGGCCAT
    GCAGATGCAG CAATGATTGC AGAATGGAAA GATAACTTAA ATGAGAGCTG GGCAGACTTG
    CTGGAACTTA TAGATACCCG TGTTCAGCTG CTGTCTGCAT CCTATGAACT CCATAAATTC
    TTCTATGATG GTACAGAAAT CCTAAGCATG ATTGAGGAGA AGCACAAGGA ATTGCCAGAA
    GATTTGGGGG GAGATGTACA CACAGCACAA TCACTTCACC GAGTGCATAC TGCATTTGAA
    AGAGATATTC ATTTTATTGG GAGCCAGGTA CAACACTTCC AAGACACAGC AGCACGTTTA
    TATGCTGCCT ATGCAGGAAC ACAGGCTGTG GATATACAGA AAAAGGAGCA AGAAGTTGTT
    GAAGCCTGGA AAGGCCTTCT GGATGCCTGT GATGGGCGTC GAACAGAGTT GATTAGTGCA
    GCAGAGAAAT TTCGGTTTTT CAGCATGGTG CGTGATTTGA TGCTTTGGAT GGAGAACATG
    ATTAGACAGA TTGAGACACA GGAAAAACCA AGAGATATAT CATCTGTTGA GTACCTAATA
    AAATACCACC AAGAACTAAA GGCAGAAATC GAAGCTAGAA ATGTCACATA TACAGCATGT
    GTGGATTTGG GAAACACTCT CTTGGCTCGT AATCACCCAG CTTCTGAGGA GATAAAAGAA
    AAGTTGGTGC AGCTAGAAGA ACGGAGAGAG GAGATGATGG AGAAGTGGCA CAAGCGCTGG
    GACTGGCTTA GTATTTTACT AGAGGTTTGC CAGTTTGCAA GAGACGCCTC CATGGCAGAA
    TCATGGTTAA TATCAAAGGA GCCATACCTG TACAGTACTT ATGTGGGAAA CAGCGTTGAT
    GATGTGGAAA AGTTAATCAG AAGGCATGAA TCATTTGAGA AATCCACTTT AACATGGGAA
    GAGAGATTTT CTGCGCTAGA AAGGCTAACA ACACTGGAGC TATTAGAAGT AAGGAAATAC
    CAGGAAGCTA AAAGACATGA AATGTTAATG GCTGCAGAAT CAGAAATGAA AGAGACAGCC
    CAGAAGGAAC AACAATTCCA AACACATGAA GAAAAAGAAC ATGAAGTGAA AGCCATTATC
    CATGCTGCCC CTGACTCTCC AGTGTTAGAG GAGCTAAAAC CAAGATTGCC CAGTATAACA
    GAAGTAACTG AAGAAACTAA GGAGAAAGAA GAGGAGAAGA TCACTCAGGA AGAGTATACA
    CATACCGAAT CCATGGAACA GACTGAATAT AGCATTTTAG ATCCAGAAAC CAGACAAATC
    GAAGCAACAA CTTTACCAAC AGATTCTGGT AGCTCTAGCA GTATTCAGCA TCAGGGTTAC
    CTAAACAGGA AACATGACCT TGATGGAACA CTTAAAAAAG CAGCAAACAG GTCCTGGAAC
    AATGTCTACT GTGTGCTTAA GAACCATGAT TTGTACTTTT ACAAAGATGC CAAATGCTTT
    GCTTTGAATG AAGCTTTCCA AGGTGAAGGC CCTCTGCCCC TGCGTAATGC AAGCTGTGAA
    ATAGTGGCCG ACTATAAAAA GAAAAAACAC GTCTTTAGGC TTCGACTCGG TGATGGAAAT
    GAATGGCTAT TTCAAGCGAA AGATGAGGCT GATCTTCAAA CCTGGGTGCC GATTCTTGCA
    ACAACCATTA CAGAGGCTAC AGATCATAAA CCTAAGTCCA AAAGTCTTCC TCTGCCCTCC
    ACATCTGCAT CTGAAGGAAA GCCAGAAAAG AAAGATAAAC GATTTACATT TTTCTCCTCA
    AAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917321.1
    CDS156..7184
    Translation

    Target ORF information:

    RefSeq Version XM_004917264.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, beta, erythrocytic (sptb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917264.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGACCA CTGACTTTGA TAACCTGGAA ATTGAGCAGC AGTACTGCAC TATCAACTCC 
    CGCTGGGAAA CGGTAGAGGA TGACCTTGAC AATGATAACA GTTCCGCTCG AATGTTTGAA
    AGATCTAGAA TTAAAGCCCT TGCAGATGAG CGTGAAGCCG TGCAAAAGAA AACATTTACC
    AAATGGGTCA ACTCCCATCT TGCTTTTGTA AGTTGTCGCA TTTCTGATCT CTACTTGGAT
    CTGAGAGATG GAAGGATGTT AATAAAACTT TTGGAGGTGC TATCAGGAGA ACAGCTGCCA
    AGAAGAACAA AAGGGAGAAT GCGTATCCAC TGCTTAGAAA ATGTGGACAA AGCTCTTCAT
    TTTCTTAAGG AGCAAAGAGT CCATCTTGAA AATATTGGTC CTCATGATAT TGTCAACGGC
    AATCATCGAT TGGTATTGGG CCTTATATGG ACCATCATAT TACGTTTCCA GATCCAAGAT
    ATTATAATTG AAATGAAAGG CAGTGAAACT CGCTCTGCAA AGGATGCTTT GCTATTATGG
    TGCCAGATGA AGACAGCAGG GTATCCAAAT GTTAATGTGA CTAATTTCAC ATCAAGCTGG
    AGGGATGGAC TGGCATTCAA TGCGTTAATC CATAAACACA GGCCTGACCT GTTTGATTTT
    GAAAGAATGA AAAAATCCAA TGCAAAGCAC AACCTTGAGC ATGCCTTTGA TACAGCTGAT
    CACAAATTGG GAATAACTCA GCTCCTTGAT CCAGAAGATG TCCTTACCGA AAACCCAGAT
    GAAAAATCCA TAATCACCTA CGTAGTAGCA TTTTATCATT ATTTTTCAAA GATGAAGGCC
    TTGGCTGTAG AAGGAAAAAG AATTGGCAAG GTGTTGGATA ATGCGATTGA AACAGATAAA
    ATGATCTATG AATATGAAAG CCTGACATCG GATTTGTTGA CATGGATTGA ACAAACCATC
    TTAGCCCTTA TAAATCGAAC ATTTGCAAAC TCTTTAACTG GGGTCCAACA GCAGCTCCAG
    GCATTCAGTG CATACAGAAC TACAGAAAAA CCACCTAAAT TTCAAGAAAA GGGTTATTTG
    GAGGTGCTCT TATTCACCAT TCAATCTAGA ATGAGAGAAA ACAAACAGAA AGTATATGTC
    CCTCAAGATG GGAAGCTTGT TTCTGACATC AACCGGGCAT GGGGTCGCCT TGAAAAAGCT
    GAGCATGACC GTGAGACAGC ACTGAGAAAT GAACTTATTC GACAAGAAAA GCTTGAGCAA
    CTGGCAAAGC GGTTCGATCG CAAGGCTGCA ATGCGAGAAA CTTGGATTTC GGAAAACCAG
    CACCTCATTG CACAGGATAA TTTTGGTTTT GATTTGCCAT CAGTGGAAGC CGCCAAGAAG
    AAACATGAAG CTATTGAGAC GGACATCTAT GCATATGAAG AACGTATACA AGCACTAGTA
    AATGTAGCTA AGGAACTGGA GAATGAGAGA TACCATGATA TCAGGCGTAT TAGTGCCAGA
    AAGGACAACA TTGTGCGCCT GTGGCAGTAT TTACTGGAGA TACTGAAGTC TAGGCGCAAT
    AGACTTAACA TGACTGTTGA ACTGCAAACC TTGTTTCAAG AGATGCTGCA CATTGTAAAC
    TGGATGGATG AAATAAAGGG AGGCCTTTTA TCCCAAGATT ATGGTAAACA TCTCCTCGAG
    GTGGAATATT TACTGCAGAA ACACAGCTTG ACAGAAGCTG ATATTGCTGC TCAGGCGGAT
    AAAGTAAAAT CATTTAGCCA GGCTGCTCTG AAGTTTGTAG GTGGAGATGG GTATCAGCCA
    TGCGATCCAC AGGTGATTAA AGATCGAGTT CAGCACCTTG AACTTTGTTA TCAGGAACTA
    GTATCACTGG CAGCACAGAG GAGGCATAAT TTGCAGCAAT CACGTTGTTT GTGGAAGTTT
    TTTGGAGACA TTGAAGAGGT GGCAAGATGG ATAAGGGAGA AAGAACAAAT CTATACTTCA
    ATGGATTTTG GAAAAGACCT AACCAGCATC TCCATTCTCC AAAGAAAACA CAGGGCTTTT
    GAGGATGAAC TCAAAGGTCT TGAAACTAAT TTGCAGCACA CTCTTAAAGA TGGAGAACAA
    ATGATTGCCA ATAAACACTA TGCGTCACCG AAAATCAAAG AACAAATTGA AAATATCAAA
    GTTCAGTGGG CTCAGCTAAA GGATTTGGCA GCCTTCCGTT TGAAAATTCT TTGTGACACT
    GAGAACTTCT TTCAGTTCCA GGTTGATGCT GATGATCTGG CTGCACGGAT GCAGGACACC
    TATCGTATGA TGCAAAGTGA CGATGTTGGT CATGATGAAT ATTCAACCAA AATATTAGTG
    AAAAAGCAAA AGGATCTCTT AGATGACATA ATGATCAATA GAAAGGTTCT GGAGGGTCTC
    AGTGAGGTGG TTCAAAGATT CCCAGAGGAG TTCAAGACTT CACCTGAGAT TGATAGTCGA
    CTACAAAAAT TAAGAGCTCT CTTTTCTGAT TTAGCATCCC TAGCAGACTT GCGTTCCCAA
    AAACTTCAAG ATGCTCTCTC ATTTTATACC GTTCTTGGTG ACATAGATGC ATGCGAGATG
    TGGATGAATG AAAAGGAGAA ATGGCTGGAA AAGATGGAGA TTCCAGAATC ACTAGAGGAC
    ATGGGAGTTG TACAGCATAG ATTTAATACA TTAGACCAAG AAATGGGTGC TCTGGGCTCC
    CAAATTCAGG ATGTGAACAC AGCAGCCAAT GCTCTTATTG AAAGTGGACA TCCCAGTAGG
    AAAAAAGTAA AGGAAGATCT GGATCACATG AATAGCAGAT GGACAGTGTT TCAAGAGATG
    GTTTCCCAAA AGCGAAGAGC AGTTGATTCT GCCCTTGGCC TTCACAATTA TGGTGTGGAA
    TGTGATGAGA CCAAGAACTG GATAATCGAA AAAATCAAGG TTGTAGAATC TATTCAGGAT
    CTTGGAAGTG ATCTTGCGTC TGTGATGTCA ATTCAAAGGA AACTCTATGG CATTGAGAGA
    GATCTAGCCG CTATTGAGGC CAAACTCATA AATCTACAAG GAGATGCACA AAAACTTGCA
    AATGATCACC CTGATCAAGC TCAAAACATC CTTGATCGAC TGATTGATAT AAATAAGAAA
    TGGGAAGAAT TGCAGAAGGC ACTGCAAAAT CAAGAGGAGT CCTTAGGAGA AAGTAGCAAA
    GTACAAAAAT TCTTGGTGGA CTTGTTTGAT TTTGAGACAT GGCTTTACAG AGCCCAACAG
    GCAACAGCTT CAGAAGATAT ACCCACTTCA CTGCCAGAGG CCGAAAAGCT ATATGCTAAC
    CACCTTTCTC TTAAAGATGA CATTGAACGC CATGAACCCG ATTTCTGTGA TGTTGTAGAT
    ACAGGTAACA AGGTAACCTC AGGACAAACA GACCCCCAAT ATGAACAACT TGATCAAAGA
    GGCAACGATA TAAAAGAAGG CTGGAATGAT CTCCACAAGA TGTGGGACAA TCGAGAGAAA
    TCCCTTTCTC AGTGCTTGGA ATTCCAGAAG TTCCTGAAGG ATGCAAAGCA GTCTGAAGCT
    ATTTTGAGTA ATCAGGAATA CACATTTGCT CATATTGAAG TTCCCACCAC ATTACAAGCT
    TCACAAACTA GACTGAAAAA ACATGAGGAT TTCCTAGCTA CTATGGAGAA TAATGACGAG
    AAGATTGTTG GAACCATCAA TCATGGAAAG AAATTAGATG AGGATAAAAA TGTTTTTGCT
    GATAAAGCTT TGGGCAAGAT TTCCTTTATT GAAGAAAGAT ATGCCAAGAA CTTAAACAAG
    GCCAAAGAAG TTTCTATTCT GCTGAAGGAT AACTGCGACC TCCAAAATTT CCTTGAAAAC
    TGCGAAGAGC TCAATATATG GATCAATGAA AAAATGTTGA CTGCTCAAGA CACATCTTAT
    GATGGAGCTC GAAACCTGCA CAGCAAATGG CTAAAGCACC AGGCTTTTAA GGCAGAACTT
    GATTCCAACA AAGAGAGACT TGAGAATCTT GACAAGGAAG GGAAACAACT GGCACAGGAA
    AAAGAACAGT TTGCACCAAT TATTTCTGAG AAACTGAGTG AGTTGCACAG ACTCTGGGAT
    GAACTGGAAA CTACCACCCT CCAAAAAGAA CAGTCTCTTT TTGATGCCCA CCGCACAGAG
    CTTTATGAGC AAAGATATGC AGATCTAAAT AAATGGATAA AAGATATGGA GCAGCAAGTA
    AAATCTGATG ACTGTGGAAC AGACCTTACA AGTGTTAATA TTCTACTAAA TAAGCACAAA
    AGAATTGAAG ATCAGATTCA ACAAAAGACA ATAGAAATGG ATCAAGTTCT ACCACAAGCT
    TCAGCTCTGG GCAAAGAACC TGATGAGAAG GAGCCAGAAA TTAACGTACG TTTTCTGCAA
    CTTGAGCCGC TAATGCAGGA GAGAAAGAGG AAACTTCTTT CTTCAAAGCA AGTACATCAA
    CTGCACCGGG ACTTGGAAGA TGAAATTCTT TGGATTCAGG AGAGATTGCC TCTTGCAGAG
    TCACAGGATT ATGGAAATAA CCTTCAGACG GTACAAATGC TAATGAAGAA AAATCAGACT
    TTACAGAAAG AGATTGCAGG TCACAGTCCC CGGATAGAGG ATGTTCTAAT GAGAGCAAAG
    CATATAGAAC ATGATGCAGA TATTGATGAT CAGCCTATTG AAAAGAAACG GAAGTCTTTG
    GAAGAGAGCT GGGATAACTT ACAGAAACAA ATAGCAGAAA GAGAAAAACA CTTGCTGCAA
    GCTCTTGAAG CACACCATTA TTATATGGAT GCAGGCGAGG CAGAGGCTTG GATTGATGAA
    CAACAGCTTT ACCTGATTGG AGATGAACAA CCAAAGGATG AAGAAAGTGT TGTTGCAATG
    TTGAAGAAAC ATCTCAATTT ACAACATGCC GTGGAACATT ATGGGAAGAA TATTAAGGAC
    TTGGCAGACC AGGCACAAAA ACTCCTTGCA TTAGAGCATC CTGATAGTGA ACGTATAACT
    CGGCACCAAG GCAATATAGA AAAGCAGTAC GCAGCACTGA GGGACATGGC TGATGAGAAA
    AGGCAAAAGC TGGACAATAT TTATCACATG TTTCAGCTGA GGCGTGAAGT TGAAGATCTG
    GAGCAATGGA TTGCAGAAAG AGATGTGAAG GCCTCATCCC AGGAAATGGG ACAAGATTTG
    GATCATGTGT CAATCCTGAG AGACAAATTC CGTGACTTTG CGAGAGAGAC AGGAGTCATT
    GGGCAGGAGC GTATTGATAA CGTCAATATT TTAATTGAAC AACTGATTGA TGCTGGCCAT
    GCAGATGCAG CAATGATTGC AGAATGGAAA GATAACTTAA ATGAGAGCTG GGCAGACTTG
    CTGGAACTTA TAGATACCCG TGTTCAGCTG CTGTCTGCAT CCTATGAACT CCATAAATTC
    TTCTATGATG GTACAGAAAT CCTAAGCATG ATTGAGGAGA AGCACAAGGA ATTGCCAGAA
    GATTTGGGGG GAGATGTACA CACAGCACAA TCACTTCACC GAGTGCATAC TGCATTTGAA
    AGAGATATTC ATTTTATTGG GAGCCAGGTA CAACACTTCC AAGACACAGC AGCACGTTTA
    TATGCTGCCT ATGCAGGAAC ACAGGCTGTG GATATACAGA AAAAGGAGCA AGAAGTTGTT
    GAAGCCTGGA AAGGCCTTCT GGATGCCTGT GATGGGCGTC GAACAGAGTT GATTAGTGCA
    GCAGAGAAAT TTCGGTTTTT CAGCATGGTG CGTGATTTGA TGCTTTGGAT GGAGAACATG
    ATTAGACAGA TTGAGACACA GGAAAAACCA AGAGATATAT CATCTGTTGA GTACCTAATA
    AAATACCACC AAGAACTAAA GGCAGAAATC GAAGCTAGAA ATGTCACATA TACAGCATGT
    GTGGATTTGG GAAACACTCT CTTGGCTCGT AATCACCCAG CTTCTGAGGA GATAAAAGAA
    AAGTTGGTGC AGCTAGAAGA ACGGAGAGAG GAGATGATGG AGAAGTGGCA CAAGCGCTGG
    GACTGGCTTA GTATTTTACT AGAGGTTTGC CAGTTTGCAA GAGACGCCTC CATGGCAGAA
    TCATGGTTAA TATCAAAGGA GCCATACCTG TACAGTACTT ATGTGGGAAA CAGCGTTGAT
    GATGTGGAAA AGTTAATCAG AAGGCATGAA TCATTTGAGA AATCCACTTT AACATGGGAA
    GAGAGATTTT CTGCGCTAGA AAGGCTAACA ACACTGGAGC TATTAGAAGT AAGGAAATAC
    CAGGAAGCTA AAAGACATGA AATGTTAATG GCTGCAGAAT CAGAAATGAA AGAGACAGCC
    CAGAAGGAAC AACAATTCCA AACACATGAA GAAAAAGAAC ATGAAGTGAA AGCCATTATC
    CATGCTGCCC CTGACTCTCC AGTGTTAGAG GAGCTAAAAC CAAGATTGCC CAGTATAACA
    GAAGTAACTG AAGAAACTAA GGAGAAAGAA GAGGAGAAGA TCACTCAGGA AGAGTATACA
    CATACCGAAT CCATGGAACA GACTGAATAT AGCATTTTAG ATCCAGAAAC CAGACAAATC
    GAAGCAACAA CTTTACCAAC AGATTCTGGT AGCTCTAGCA GTATTCAGCA TCAGGGTTAC
    CTAAACAGGA AACATGACCT TGATGGAACA CTTAAAAAAG CAGCAAACAG GTCCTGGAAC
    AATGTCTACT GTGTGCTTAA GAACCATGAT TTGTACTTTT ACAAAGATGC CAAATGCTTT
    GCTTTGAATG AAGCTTTCCA AGGTGAAGGC CCTCTGCCCC TGCGTAATGC AAGCTGTGAA
    ATAGTGGCCG ACTATAAAAA GAAAAAACAC GTCTTTAGGC TTCGACTCGG TGATGGAAAT
    GAATGGCTAT TTCAAGCGAA AGATGAGGCT GATCTTCAAA CCTGGGTGCC GATTCTTGCA
    ACAACCATTA CAGAGGCTAC AGATCATAAA CCTAAGTCCA AAAGTCTTCC TCTGCCCTCC
    ACATCTGCAT CTGAAGGAAA GCCAGAAAAG AAAGATAAAC GATTTACATT TTTCTCCTCA
    AAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.