SPTB gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005268023.4	XP_005268080.1	spectrin beta chain, erythrocytic isoform X1
XM_017021613.1	XP_016877102.1	spectrin beta chain, erythrocytic isoform X2
NM_001355436.2	NP_001342365.1	spectrin beta chain, erythrocytic isoform a
NM_001355437.2	NP_001342366.1	spectrin beta chain, erythrocytic isoform b
XM_024449699.1	XP_024305467.1	spectrin beta chain, erythrocytic isoform X1
XM_011537105.3	XP_011535407.1	spectrin beta chain, erythrocytic isoform X2
NM_001355436.1	NP_001342365.1	spectrin beta chain, erythrocytic isoform a
NM_001024858.2	NP_001020029.1	spectrin beta chain, erythrocytic isoform a
XM_011537105.2	XP_011535407.1	spectrin beta chain, erythrocytic isoform X4
NM_001024858.3	NP_001020029.1	spectrin beta chain, erythrocytic isoform a
NM_001355437.1	NP_001342366.1	spectrin beta chain, erythrocytic isoform b
NM_000347.5	NP_000338.3	spectrin beta chain, erythrocytic isoform b
XM_017021612.1	XP_016877101.1	spectrin beta chain, erythrocytic isoform X1
XM_017021614.1	XP_016877103.1	spectrin beta chain, erythrocytic isoform X3
XM_017021612.2	XP_016877101.1	spectrin beta chain, erythrocytic isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu60719	XM_011537105.3 Latest version!	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu85086	XM_017021613.1 Latest version!	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu02426	NM_000347.5 Latest version!	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu02426	NM_001355437.2 Latest version!	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu17941	NM_001024858.3 Latest version!	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu17941	NM_001355436.2 Latest version!	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu02426	XM_017021614.1 Latest version!	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu17941	XM_024449699.1 Latest version!	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu17941	XM_005268023.4 Latest version!	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu17941	XM_017021612.2 Latest version!	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
View Old Accession Versions >>
OHu17941	NM_001355436.1	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu60719	XM_011537105.2	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu17941	NM_001024858.2	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu02426	NM_001355437.1	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu17941	XM_017021612.1	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
Hide Old Accession Versions >>

SPTB ( XM_011537105.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535407.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu60719
Clone ID Related Accession (Same CDS sequence)	XM_011537105.3 , XM_011537105.2
Accession Version	XM_011537105.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011537105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGGAATCAA GCTCTGCTGA AGCACAGGAA GATAGGGGAC AGTCAAGAGC ACAGGCTGCC 
AGGTCAGGTG GAGCTGTGTT CAACTCCGGC TCTGCCACTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535407.1
CDS	121..4785
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQESSSAEAQEDRGQSRAQAARSGGAVFNSGSATE

Target ORF information:

RefSeq Version	XM_011537105.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537105.3

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGGAATCAA GCTCTGCTGA AGCACAGGAA GATAGGGGAC AGTCAAGAGC ACAGGCTGCC 
AGGTCAGGTG GAGCTGTGTT CAACTCCGGC TCTGCCACTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_011537105.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535407.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu60719
Clone ID Related Accession (Same CDS sequence)	XM_011537105.3 , XM_011537105.2
Accession Version	XM_011537105.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGGAATCAA GCTCTGCTGA AGCACAGGAA GATAGGGGAC AGTCAAGAGC ACAGGCTGCC 
AGGTCAGGTG GAGCTGTGTT CAACTCCGGC TCTGCCACTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535407.1
CDS	122..4786
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQESSSAEAQEDRGQSRAQAARSGGAVFNSGSATE

Target ORF information:

RefSeq Version	XM_011537105.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537105.2

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGGAATCAA GCTCTGCTGA AGCACAGGAA GATAGGGGAC AGTCAAGAGC ACAGGCTGCC 
AGGTCAGGTG GAGCTGTGTT CAACTCCGGC TCTGCCACTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_017021613.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877102.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X3, mRNA.

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CloneID	OHu85086
Clone ID Related Accession (Same CDS sequence)	XM_017021613.1
Accession Version	XM_017021613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG TAAGCGCGGG CAGCCTAGCC 
CAGACCTGGC TAAGAAGGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877102.1
CDS	122..6985
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLPAPRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRHAICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEVSAGSLAQTWLRRG

Target ORF information:

RefSeq Version	XM_017021613.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021613.1

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG TAAGCGCGGG CAGCCTAGCC 
CAGACCTGGC TAAGAAGGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( NM_001355437.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001342366.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 2, mRNA.

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CloneID	OHu02426
Clone ID Related Accession (Same CDS sequence)	NM_001355437.2 , NM_001355437.1 , NM_000347.5 , XM_017021614.1
Accession Version	NM_001355437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL135745.5 and AL121774.5. This sequence is a reference standard in the RefSeqGene project. On May 11, 2018 this sequence version replaced NM_001355437.1. Transcript Variant: This variant (2) differs in the 3' UTR and 3' coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. This variant is also referred to as muscle beta spectrin. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: J05500.1, BC136284.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGTCAG TCTCTGGTCT 
CGTTTGTCTA GTTCCTGGGA GTCACTGCAG CCAGAGCCCT CTCACCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342366.1
CDS	168..6581
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPVSLWSRLSSSWESLQPEPSHPY

Target ORF information:

RefSeq Version	NM_001355437.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355437.2

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGTCAG TCTCTGGTCT 
CGTTTGTCTA GTTCCTGGGA GTCACTGCAG CCAGAGCCCT CTCACCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( NM_001355437.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001342366.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 2, mRNA.

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CloneID	OHu02426
Clone ID Related Accession (Same CDS sequence)	NM_001355437.2 , NM_001355437.1 , NM_000347.5 , XM_017021614.1
Accession Version	NM_001355437.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL135745.5 and AL121774.5. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 3' UTR and 3' coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. This variant is also referred to as muscle beta spectrin. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J05500.1, BC136284.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGTCAG TCTCTGGTCT 
CGTTTGTCTA GTTCCTGGGA GTCACTGCAG CCAGAGCCCT CTCACCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342366.1
CDS	144..6557
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPVSLWSRLSSSWESLQPEPSHPY

Target ORF information:

RefSeq Version	NM_001355437.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355437.1

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGTCAG TCTCTGGTCT 
CGTTTGTCTA GTTCCTGGGA GTCACTGCAG CCAGAGCCCT CTCACCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( NM_000347.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000338.3 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 2, mRNA.

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CloneID	OHu02426
Clone ID Related Accession (Same CDS sequence)	NM_001355437.2 , NM_001355437.1 , NM_000347.5 , XM_017021614.1
Accession Version	NM_000347.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-07
Organism	Homo sapiens(Human)
Product	spectrin beta chain, erythrocytic isoform b
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB209415.1, BM681142.1 and X59511.1. This sequence is a reference standard in the RefSeqGene project. On Nov 11, 2009 this sequence version replaced NM_000347.4. Transcript Variant: This variant (2) differs in the 3' UTR and 3' coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. This variant is also referred to as muscle beta spectrin. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J05500.1, BC136284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGTCAG TCTCTGGTCT 
CGTTTGTCTA GTTCCTGGGA GTCACTGCAG CCAGAGCCCT CTCACCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000338.3
CDS	55..6468
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPVSLWSRLSSSWESLQPEPSHPY

Target ORF information:

RefSeq Version	NM_000347.5
Organism	Homo sapiens(Human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000347.5

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGTCAG TCTCTGGTCT 
CGTTTGTCTA GTTCCTGGGA GTCACTGCAG CCAGAGCCCT CTCACCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( NM_001355436.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001342365.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 1, mRNA.

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CloneID	OHu17941
Clone ID Related Accession (Same CDS sequence)	XM_005268023.4 , NM_001355436.2 , XM_024449699.1 , NM_001355436.1 , NM_001024858.2 , NM_001024858.3 , XM_017021612.1 , XM_017021612.2
Accession Version	NM_001355436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL135745.5 and AL121774.5. This sequence is a reference standard in the RefSeqGene project. On May 11, 2018 this sequence version replaced NM_001355436.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB209415.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342365.1
CDS	168..7154
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLPAPRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRHAICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWLQGVSTAINESQSIRVKAQSLPLPSLSGPDASLGKKDKEKRFSFFPKKK

Target ORF information:

RefSeq Version	NM_001355436.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355436.2

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( NM_001355436.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001342365.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 1, mRNA.

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CloneID	OHu17941
Clone ID Related Accession (Same CDS sequence)	XM_005268023.4 , NM_001355436.2 , XM_024449699.1 , NM_001355436.1 , NM_001024858.2 , NM_001024858.3 , XM_017021612.1 , XM_017021612.2
Accession Version	NM_001355436.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL135745.5 and AL121774.5. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209415.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342365.1
CDS	144..7130
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLPAPRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRHAICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWLQGVSTAINESQSIRVKAQSLPLPSLSGPDASLGKKDKEKRFSFFPKKK

Target ORF information:

RefSeq Version	NM_001355436.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355436.1

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( NM_001024858.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020029.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 1, mRNA.

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CloneID	OHu17941
Clone ID Related Accession (Same CDS sequence)	XM_005268023.4 , NM_001355436.2 , XM_024449699.1 , NM_001355436.1 , NM_001024858.2 , NM_001024858.3 , XM_017021612.1 , XM_017021612.2
Accession Version	NM_001024858.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-07
Organism	Homo sapiens(Human)
Product	spectrin beta chain, erythrocytic isoform a
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB209415.1, M37884.1, BX248270.1, AK094815.1, CN370674.1 and BC010434.1. This sequence is a reference standard in the RefSeqGene project. On Nov 11, 2009 this sequence version replaced NM_001024858.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209415.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020029.1
CDS	55..7041
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLPAPRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRHAICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWLQGVSTAINESQSIRVKAQSLPLPSLSGPDASLGKKDKEKRFSFFPKKK

Target ORF information:

RefSeq Version	NM_001024858.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024858.2

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( NM_001024858.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020029.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 3, mRNA.

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CloneID	OHu17941
Clone ID Related Accession (Same CDS sequence)	XM_005268023.4 , NM_001355436.2 , XM_024449699.1 , NM_001355436.1 , NM_001024858.2 , NM_001024858.3 , XM_017021612.1 , XM_017021612.2
Accession Version	NM_001024858.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121774.5, AB209415.1, M37884.1, BX248270.1, AK094815.1, CN370674.1 and BC010434.1. On Dec 20, 2017 this sequence version replaced NM_001024858.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209415.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020029.1
CDS	287..7273
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLPAPRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRHAICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWLQGVSTAINESQSIRVKAQSLPLPSLSGPDASLGKKDKEKRFSFFPKKK

Target ORF information:

RefSeq Version	NM_001024858.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024858.3

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_017021614.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877103.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X4, mRNA.

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CloneID	OHu02426
Clone ID Related Accession (Same CDS sequence)	NM_001355437.2 , NM_001355437.1 , NM_000347.5 , XM_017021614.1
Accession Version	XM_017021614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGTCAG TCTCTGGTCT 
CGTTTGTCTA GTTCCTGGGA GTCACTGCAG CCAGAGCCCT CTCACCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877103.1
CDS	122..6535
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPVSLWSRLSSSWESLQPEPSHPY

Target ORF information:

RefSeq Version	XM_017021614.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021614.1

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGTCAG TCTCTGGTCT 
CGTTTGTCTA GTTCCTGGGA GTCACTGCAG CCAGAGCCCT CTCACCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_005268023.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268080.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X1, mRNA.

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CloneID	OHu17941
Clone ID Related Accession (Same CDS sequence)	XM_005268023.4 , NM_001355436.2 , XM_024449699.1 , NM_001355436.1 , NM_001024858.2 , NM_001024858.3 , XM_017021612.1 , XM_017021612.2
Accession Version	XM_005268023.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268023.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268080.1
CDS	122..7108
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLPAPRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRHAICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWLQGVSTAINESQSIRVKAQSLPLPSLSGPDASLGKKDKEKRFSFFPKKK

Target ORF information:

RefSeq Version	XM_005268023.4
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268023.4

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_024449699.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305467.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X1, mRNA.

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CloneID	OHu17941
Clone ID Related Accession (Same CDS sequence)	XM_005268023.4 , NM_001355436.2 , XM_024449699.1 , NM_001355436.1 , NM_001024858.2 , NM_001024858.3 , XM_017021612.1 , XM_017021612.2
Accession Version	XM_024449699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305467.1
CDS	412..7398
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLPAPRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRHAICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWLQGVSTAINESQSIRVKAQSLPLPSLSGPDASLGKKDKEKRFSFFPKKK

Target ORF information:

RefSeq Version	XM_024449699.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449699.1

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_017021612.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877101.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X2, mRNA.

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CloneID	OHu17941
Clone ID Related Accession (Same CDS sequence)	XM_005268023.4 , NM_001355436.2 , XM_024449699.1 , NM_001355436.1 , NM_001024858.2 , NM_001024858.3 , XM_017021612.1 , XM_017021612.2
Accession Version	XM_017021612.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877101.1
CDS	270..7256
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLPAPRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRHAICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWLQGVSTAINESQSIRVKAQSLPLPSLSGPDASLGKKDKEKRFSFFPKKK

Target ORF information:

RefSeq Version	XM_017021612.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021612.1

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_017021612.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877101.1 Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X2, mRNA.

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CloneID	OHu17941
Clone ID Related Accession (Same CDS sequence)	XM_005268023.4 , NM_001355436.2 , XM_024449699.1 , NM_001355436.1 , NM_001024858.2 , NM_001024858.3 , XM_017021612.1 , XM_017021612.2
Accession Version	XM_017021612.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, erythrocytic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021612.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877101.1
CDS	267..7253
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLPAPRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRHAICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWLQGVSTAINESQSIRVKAQSLPLPSLSGPDASLGKKDKEKRFSFFPKKK

Target ORF information:

RefSeq Version	XM_017021612.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021612.2

ORF Insert Sequence:

ATGACATCGG CCACAGAGTT TGAAAATGTG GGCAACCAGC CACCTTACAG CAGGATCAAT 
GCCCGCTGGG ACGCCCCAGA CGACGAGCTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATAAAGGC CTTGGCAGAT GAGCGGGAAG TTGTTCAGAA AAAGACCTTC 
ACGAAATGGG TGAACTCGCA CCTGGCTCGA GTGTCCTGCC GCATCACCGA TCTCTACAAG 
GACCTGCGGG ATGGGCGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAAGCCCA CCAAGGGGAA GATGCGCATC CACTGCCTGG AGAATGTGGA CAAGGCTCTC 
CAGTTCCTCA AGGAGCAGCG TGTACACCTG GAGAACATGG GCTCCCACGA CATTGTAGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTCCGCTT CCAGATTCAG 
GACATTGTGG TCCAAACTCA GGAAGGTCGT GAAACACGCT CAGCCAAGGA TGCGTTGCTG 
TTGTGGTGTC AGATGAAGAC GGCAGGCTAC CCTCATGTTA ATGTCACCAA CTTTACCTCC 
AGCTGGAAGG ATGGCTTGGC CTTTAATGCC CTGATACACA AGCACCGGCC CGACCTGATC 
GACTTTGATA AGCTGAAGGA CTCCAATGCC CGGCACAACC TGGAGCACGC ATTCAATGTG 
GCTGAGCGCC AGCTGGGCAT CATCCCGCTC CTCGACCCCG AAGATGTCTT TACGGAAAAC 
CCTGATGAGA AATCCATCAT CACCTATGTG GTGGCCTTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTGG CAGTGGAGGG CAAGCGTGTC GGCAAGGTTA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGCGGGCTA GCCTCGGACC TGCTCACCTG GATCGAGCAG 
ACCATCACTG TCCTGAACAG CCGCAAGTTT GCCAACTCGC TGACGGGCGT CCAGCAGCAG 
CTGCAGGCCT TCAGCACCTA CCGCACCGTG GAGAAGCCGC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TTCTACTTTT TACCATCCAG TCCCGGATGA GAGCCAACAA TCAGAAAGTG 
TACACACCCC ACGATGGGAA ACTAGTGTCT GACATCAACA GGGCCTGGGA AAGCCTGGAG 
GAAGCTGAGT ATCGGCGGGA GCTGGCCCTG AGAAATGAGC TCATTCGGCA GGAGAAGCTA 
GAGCAACTGG CCCGGCGCTT TGACCGGAAG GCCGCAATGA GAGAGACCTG GCTCAGTGAA 
AACCAGCGCC TCGTGGCCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ATGAGGCCAT CGAGACCGAC ACGGCTGCCT ACGAGGAGCG GGTGAGAGCC 
CTGGAGGACC TGGCTCAGGA GCTGGAGAAA GAGAACTACC ATGACCAGAA GCGCATCACG 
GCCCGCAAGG ACAATATACT GCGCCTATGG AGCTACCTGC AGGAGCTGCT GCAGTCCCGG 
CGCCAGAGGC TCGAGACCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGC 
ATCGACTGGA TGGATGAGAT CAAGGCTCAC CTCTTGTCTG CCGAGTTTGG GAAGCACTTG 
TTGGAGGTTG AAGACCTGCT ACAGAAGCAC AAGTTGATGG AAGCTGACAT CGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACCGCAGCC ACCCTGAAGT TCACCGAGGG GAAAGGGTAC 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGCATCAGCC ACTTGGAGCA GTGCTTTGAG 
GAGCTGAGCA ACATGGCAGC TGGGCGGAAG GCCCAACTGG AGCAGTCCAA ACGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCTGAG AGCTGGATCA AGGAGAAGGA GCAGATCTAT 
TCTTCCCTGG ACTATGGCAA AGACCTGACC AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAT GCTCACCTGG AGCAGATCTT CCAGGAGGCT 
CATGGCATGG TTGCGCGCAA GCAGTTTGGG CACCCGCAGA TCGAGGCCCG CATAAAGGAG 
GTGTCGGCAC AGTGGGACCA GCTGAAGGAC CTGGCTGCCT TCTGCAAGAA GAACCTCCAG 
GATGCTGAGA ACTTTTTCCA GTTCCAGGGC GATGCGGATG ACCTGAAGGC TTGGCTGCAA 
GACGCCCACC GGCTGCTCTC TGGTGAAGAT GTGGGGCAGG ACGAAGGGGC CACGCGGGCC 
CTGGGGAAAA AGCACAAGGA CTTCCTGGAG GAGCTGGAGG AGAGCCGTGG GGTGATGGAG 
CACCTGGAGC AGCAGGCCCA GGGATTCCCC GAAGAGTTTC GGGATTCCCC AGATGTGACC 
CATCGGCTGC AGGCCCTGCG GGAGCTCTAC CAACAGGTGG TGGCCCAGGC GGACCTGCGT 
CAGCAGAGGC TGCAGGAAGC CCTGGACCTG TACACGGTGT TCGGGGAGAC AGACGCCTGT 
GAGCTGTGGA TGGGAGAGAA GGAGAAGTGG CTGGCCGAGA TGGAAATGCC AGACACCCTG 
GAGGACCTGG AGGTCGTGCA GCACAGGTTC GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGACTCAGA TTGATGGTGT GAACCTCGCT GCCAACAGCT TGGTAGAGAG TGGCCACCCA 
CGCAGCAGGG AGGTGAAGCA GTACCAGGAC CATCTGAACA CCAGGTGGCA GGCATTTCAG 
ACCCTGGTGT CGGAGCGGCG GGAGGCTGTG GACTCAGCCC TCCGAGTGCA CAACTACTGC 
GTAGATTGCG AGGAGACCAG CAAGTGGATC ACGGACAAGA CAAAGGTAGT GGAGTCCACA 
AAAGACCTGG GGCGGGACCT GGCAGGTATC ATCGCCATCC AGAGGAAGTT GTCAGGGCTG 
GAGCGTGACG TGGCCGCCAT CCAGGCCCGT GTGGATGCCC TGGAGCGTGA GTCCCAGCAG 
CTGATGGACT CGCACCCTGA GCAGAAGGAG GATATTGGTC AGCGGCAAAA ACACTTGGAG 
GAGCTGTGGC AGGGCCTGCA GCAATCCCTG CAGGGCCAGG AGGACTTGCT GGGGGAAGTC 
AGCCAGCTGC AGGCCTTCCT GCAGGATCTG GATGACTTCC AGGCCTGGCT CTCCATCACC 
CAGAAGGCTG TGGCCTCTGA GGACATGCCC GAATCCCTCC CAGAGGCTGA GCAGCTCCTG 
CAGCAGCATG CAGGTATCAA GGATGAGATT GACGGGCACC AAGACAGCTA CCAGCGTGTT 
AAGGAGTCTG GGGAGAAAGT GATCCAAGGC CAGACGGACC CAGAGTATCT GCTTCTGGGC 
CAGCGGCTGG AGGGCCTGGA TACTGGCTGG AATGCCCTGG GCAGGATGTG GGAGAGCCGC 
AGCCACACCC TCGCTCAGTG CCTTGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCCCC AGACTCCCTG 
GAAGCTGCAG AGGCTGGGAT CCGGAAGTTT GAGGATTTCT TGGGGTCTAT GGAGAACAAC 
CGGGATAAGG TCTTGAGTCC TGTGGACTCT GGAAACAAGC TGGTAGCTGA GGGAAACCTA 
TACTCAGACA AGATCAAGGA GAAGGTGCAG CTGATTGAGG ACAGGCACAG GAAGAACAAC 
GAGAAGGCCC AGGAGGCCTC TGTCCTACTG AGAGACAACC TGGAGCTACA GAACTTCCTC 
CAGAACTGCC AGGAGCTCAC TCTCTGGATC AACGACAAGC TGCTGACATC TCAGGATGTC 
TCCTATGATG AAGCACGAAA CCTTCACAAT AAATGGCTAA AGCACCAGGC GTTTGTGGCA 
GAGCTGGCTT CCCATGAAGG GTGGCTAGAG AACATCGATG CGGAAGGAAA GCAGCTGATG 
GATGAGAAGC CCCAGTTTAC AGCCCTGGTG TCCCAAAAGC TGGAAGCCCT GCACCGGCTC 
TGGGACGAGC TGCAGGCCAC CACAAAGGAG AAGACCCAGC ACCTCTCGGC TGCCAGGAGC 
TCCGACCTGC GCTTGCAGAC CCATGCTGAC CTCAACAAGT GGATCAGCGC CATGGAGGAC 
CAGCTGCGGT CAGACGACCC GGGCAAGGAC CTGACCAGTG TCAATCGGAT GTTGGCTAAG 
CTGAAGCGAG TGGAGGACCA AGTGAATGTG CGAAAAGAGG AGCTGGGGGA GCTGTTTGCC 
CAGGTGCCTT CAATGGGAGA GGAGGGAGGA GATGCAGACT TGAGCATCGA GAAGCGGTTC 
CTGGACCTCC TGGAACCCCT AGGAAGGAGG AAGAAGCAGC TGGAATCATC CAGAGCCAAG 
CTGCAGATCA GCCGGGACTT AGAGGATGAG ACGCTTTGGG TGGAGGAGAG GCTGCCTCTG 
GCCCAGTCAG CCGACTATGG CACTAATCTG CAAACTGTGC AACTGTTCAT GAAGAAGAAC 
CAGACACTGC AGAATGAGAT TCTGGGCCAT ACGCCGCGGG TTGAGGATGT GCTGCAGAGA 
GGGCAGCAGC TGGTGGAGGC GGCGGAGATC GACTGCCAGG ACCTTGAGGA GCGCCTGGGG 
CACCTGCAGA GCTCCTGGGA CAGGCTGCGG GAGGCAGCGG CCGGGAGGCT GCAGCGACTG 
AGGGACGCCA ACGAGGCACA GCAGTACTAC CTGGATGCAG ACGAGGCTGA GGCCTGGATT 
GGCGAGCAGG AGCTCTACGT CATCTCCGAT GAGATCCCCA AGGATGAAGA GGGCGCCATT 
GTGATGCTGA AGCGACATTT GCGGCAGCAG CGTGCGGTGG AGGACTACGG CCGGAACATC 
AAGCAGCTGG CCAGCCGGGC CCAGGGCCTG CTGTCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TTCAGGGGCA AGTGGACAAG CACTACGCAG GGCTGAAGGA CGTGGCGGAA 
GAGCGCAAGC GCAAGCTGGA GAACATGTAC CACCTGTTCC AGCTCAAGCG GGAGACCGAC 
GACCTGGAGC AGTGGATTTC AGAAAAGGAG CTAGTGGCCT CTTCCCCGGA AATGGGGCAA 
GACTTTGACC ACGTGACTCT TCTGCGGGAC AAGTTCCGGG ACTTTGCCCG GGAGACCGGG 
GCGATTGGGC AGGAGCGGGT GGACAATGTG AATGCCTTCA TCGAGCGACT CATCGACGCG 
GGCCACAGCG AGGCGGCCAC CATCGCCGAG TGGAAGGACG GGCTGAACGA GATGTGGGCA 
GACCTCCTGG AGCTCATTGA CACGCGCATG CAGCTGCTGG CCGCCTCCTA TGACCTGCAC 
CGCTACTTCT ACACGGGTGC CGAGATCCTG GGCCTCATCG ACGAGAAGCA CCGCGAGCTG 
CCCGAGGACG TGGGGCTGGA CGCCAGCACG GCCGAGTCCT TCCACCGGGT GCACACAGCC 
TTCGAGCGGG AGCTCCACCT GCTGGGTGTC CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGTCTGCAGA CAGCATATGC TGGGGAGAAG GCAGAGGCCA TCCAGAACAA GGAGCAGGAG 
GTGTCTGCCG CGTGGCAGGC GCTGCTCGAT GCCTGTGCCG GGCGCCGGAC CCAGCTAGTG 
GACACGGCGG ATAAATTCCG CTTCTTCAGC ATGGCCCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGGCCCAGGG ATGTCTCCTC TGTGGAACTG 
CTCATGAAGT ATCACCAGGG CATCAATGCA GAGATTGAAA CCCGGAGCAA GAACTTCAGT 
GCCTGCCTGG AGCTTGGCGA GTCCCTGCTG CAGCGGCAGC ACCAGGCCTC AGAGGAGATC 
CGCGAGAAAC TGCAGCAGGT GATGTCCAGG AGGAAAGAGA TGAATGAGAA GTGGGAAGCC 
CGCTGGGAGC GGCTCCGCAT GTTGCTGGAG GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCTGAGGCGT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCGGGGACTT TGGACACACA 
GTGGACAGTG TGGAGAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACGGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTGAA AGAACGCCAG 
ATTGCAGAGA GACCCGCAGA GGAGACTGGG CCTCAAGAGG AGGAAGGCGA GACAGCAGGG 
GAGGCTCCAG TTTCCCACCA TGCGGCCACC GAGAGAACGT CCCCGGGGGA AGAAGAGGGA 
ACGTGGCCTC AGAACCTGCA GCAGCCACCA CCGCCGGGTC AGCACAAGGA TGGGCAGAAA 
TCCACTGGGG ATGAGAGGCC CACCACGGAG CCCCTCTTTA AGGTCCTAGA TACGCCTCTG 
AGCGAGGGTG ATGAGCCCGC AACGCTGCCG GCCCCGCGGG ACCATGGGCA GAGTGTGCAG 
ATGGAAGGCT ACCTGGGCCG CAAGCATGAC CTGGAGGGGC CCAACAAGAA GGCTTCCAAC 
AGGTCCTGGA ACAACCTGTA CTGTGTGCTC AGGAACAGTG AGCTAACCTT CTACAAGGAT 
GCCAAGAACC TGGCCCTGGG GATGCCCTAC CATGGGGAGG AACCCCTGGC CCTGAGACAT 
GCCATCTGTG AGATTGCTGC CAACTACAAG AAGAAGAAGC ACGTCTTTAA GCTGAGGCTG 
AGTAATGGCA GCGAGTGGCT CTTCCATGGC AAGGATGAGG AGGAGATGCT GTCCTGGCTG 
CAGGGCGTGA GCACCGCCAT CAACGAGTCC CAGAGCATCC GCGTCAAGGC GCAGAGCCTG 
CCCCTGCCTT CCCTCTCCGG CCCCGACGCC AGCCTCGGCA AGAAAGACAA GGAGAAGAGA 
TTCAGCTTCT TCCCCAAAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPTB
Entrez Gene ID	6710
Full Name	spectrin beta, erythrocytic
Synonyms	EL3,HS2,HSPTB1,SPH2
General protein information	Preferred Names spectrin beta, erythrocytic Names spectrin beta chain, erythrocytic Sp beta beta-I spectrin membrane cytoskeletal protein spectrin beta Tandil spectrin beta chain, erythrocyte
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q23.3
Summary	This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009].
Disorder MIM:	182870
Disorder Html:	Elliptocytosis-3 (3); Spherocytosis, type 2, 616649 (3); Anemia, neonatal hemolytic, fatal and near-fatal (3)

	SPTB	XP_002805136.1
Rattus norvegicus (Norway rat)	Sptb	NP_997687.1
Gallus gallus (chicken)	SPTB	XP_004941320.1
Xenopus tropicalis (tropical clawed frog)	LOC100489265	XP_004917321.1
Canis lupus familiaris (dog)	SPTB	NP_001207410.1
Homo sapiens (human)	SPTB	NP_001020029.1
Bos taurus (cattle)	SPTB	XP_003586637.2
Mus musculus (house mouse)	Sptb	NP_038703.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPTB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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