Sptb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sptb
Entrez Gene ID	20741
Full Name	spectrin beta, erythrocytic
Synonyms	AI842465,D330027P03Rik,Gm1301,Spnb-1,Spnb1,ja,jaundiced,mKIAA4219
General protein information	Preferred Names spectrin beta, erythrocytic Names spectrin beta chain, erythrocytic beta-I spectrin beta-spectrin 1 brain erythroid spectrin (235E) spectrin R spectrin beta 1 spectrin beta chain, erythrocyte
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 C3\|12 33.73 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013675.3	NP_038703.3	spectrin beta chain, erythrocytic

Pathways from BioSystems

Homologies

	SPTB	XP_002805136.1
Rattus norvegicus (Norway rat)	Sptb	NP_997687.1
Gallus gallus (chicken)	SPTB	XP_004941320.1
Xenopus tropicalis (tropical clawed frog)	LOC100489265	XP_004917321.1
Canis lupus familiaris (dog)	SPTB	NP_001207410.1
Homo sapiens (human)	SPTB	NP_001020029.1
Bos taurus (cattle)	SPTB	XP_003586637.2
Mus musculus (house mouse)	Sptb	NP_038703.3

Gene Ontology
Bibliography

Related articles in PubMed

Switching of ?-Catenin From Epithelial to Neuronal Type During Lens Epithelial Cell Differentiation.
Maddala R, Rao PV
Investigative ophthalmology & visual science58(9)3445-3455(2017 Jul)

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.
Huang YS, Delgadillo LF, Cyr KH, Kingsley PD, An X, McGrath KE, Mohandas N, Conboy JG, Waugh RE, Wan J, Palis J
Scientific reports7(1)5164(2017 Jul)

A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice.
Huang HM, Bauer DC, Lelliott PM, Greth A, McMorran BJ, Foote SJ, Burgio G
Scientific reports637197(2016 Nov)

Cell organization, growth, and neural and cardiac development require ?II-spectrin.
Stankewich MC, Cianci CD, Stabach PR, Ji L, Nath A, Morrow JS
Journal of cell science124(Pt 23)3956-66(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results of this study provide evidence for a role for spectrin in different steps of megakaryocyte development through its participation in the formation of invaginated membranes and in the maintenance of proplatelet structure.
Title: The spectrin-based membrane skeleton stabilizes mouse megakaryocyte membrane systems and is essential for proplatelet and platelet formation.

The following Sptb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09774	NM_013675.3 Latest version!	Mus musculus spectrin beta, erythrocytic (Sptb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1091.30 $1559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sptb ( NM_013675.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038703.3 Mus musculus spectrin beta, erythrocytic (Sptb), mRNA.

Price & Availability↑

CloneID	OMu09774
Clone ID Related Accession (Same CDS sequence)	NM_013675.3
Accession Version	NM_013675.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, erythrocytic
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147299.1 and CD804076.1. On Jan 6, 2006 this sequence version replaced NM_013675.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147684.1, AK147299.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGACATCGG CCACGGAGTT TGAAAACGTG GGCAACCAGC CACCATTCAG CCGGATTAAC 
GCCCGCTGGG ATGCCCCCGA TGACGAACTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCAC GGATCAAGGC CTTGGCAGAT GAGCGGGAAG TGGTCCAGAA GAAAACCTTT 
ACGAAATGGG TGAACTCCCA CCTGGCTCGA GTGTCCTGCC GCATCAGCGA CCTCTACAAG 
GACCTGCGGG ACGGACGGAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAGGCCGA CCAAGGGCAA GATGCGCATC CACTGCCTGG AGAACGTGGA CAAAGCCCTG 
CAGTTCCTCA AGGAACAGCG CGTGCACCTG GAAAACATGG GCTCCCACGA CATTGTGGAT 
GGTAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTTCGATT CCAGATTCAG 
GACATTGTTG TCCAAACTCA AGAAGGTCGG GAGACACGCT CAGCCAAGGA TGCTCTGCTC 
CTGTGGTGCC AGATGAAGAC AGCAGGCTAC CCCCATGTCA ATGTAACCAA CTTCACCTCC 
AGCTGGAAGG ATGGCCTGGC CTTCAATGCC CTAATACACA AACACCGGCC TGACCTCATC 
GACTTTGACA AGCTGAAGGA CTCCAATGCC CGGCATAACC TGGAGCATGC TTTTGATGTA 
GCCGAGCGCC AGCTCGGCAT TATCCCACTC CTTGACCCCG AAGATGTCTT CACAGAAAAC 
CCCGATGAGA AATCCATCAT CACCTACGTG GTGGCGTTTT ATCACTACTT CTCCAAGATG 
AAGGTGCTGG CGGTGGAGGG CAAGCGAGTT GGCAAGGTCA TCGACCACGC CATCGAGACC 
GAGAAGATGA TTGAAAAGTA CAGTGGGCTA GCCTCAGACC TGCTCACTTG GATTGAACAA 
ACCATTAGCG TCCTAAACAG CCGCAAGTTT GCCAACTCGT TGAGTGGCGT CCAGCAGCAG 
CTACAGGCCT TCAGCACCTA CCGTACTGTG GAGAAGCCAC CCAAGTTTCA GGAGAAGGGG 
AATCTGGAAG TCCTCCTATT CACTATCCAA TCCCGGATGA GAGCCAACAA TCAGAAGGTG 
TACACGCCCC ACGATGGGAA GCTGGTGTCT GACATCAACA GGGCCTGGGA GAGCCTGGAG 
GAAGCAGAGT ACCAAAGGGA GCTGGCTCTG AGGAGCGAGC TCATTCGGCA GGAGAAGCTG 
GAGCAGCTGG CGCGGCGCTT CGACCGGAAG GCTGCCATGA GAGAGACGTG GCTCAATGAA 
AACCAGCGCC TGGTGACCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ACGAAGCCAT TGAGACCGAC ACAGCTGCCT ATGAGGAGCG GGTAAAGGCG 
CTGGAGGACC TGGCCCAGGA GCTGGAGAAG GAGAATTACC ATGACCAGAA GCGTATCATT 
GCCCGGAAGG ACAACATCCT CCGTCTGTGG AGCTACCTGC AGGAGCTGCT GCGATCCCGG 
CGACAGAGGC TGGAGGCCAC CCTGGCACTG CAGAAACTCT TTCAGGACAT GCTGCACAGC 
ATCGATTGGA TGGATGAGAT CAAGGCTCAC ATCTTGTCTG CTGAATTTGG GAAGCACTTG 
TTAGAGGTTG AAGACCTGCT GCAGAAGCAC AAGCTAATGG AGGCCGACAT TGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACAGCGGCC ACCCTGCAGT TCGCTGAGGG GAAAGGGTAT 
CAACCTTGTG ACCCCCAGGT CATCCAGGAC CGTGTCAGCC ACCTAGAGCA GTGCTTCAGT 
GAACTGAGCA ACATGGCAGC TGGACGGAAG GCTCAGCTGG AGCAGTCTAA GCGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCCGAA AGCTGGATCA AGGAGAAGGA GCAGATCTAC 
TCCTCCCTGG ACTACGGCAA GGACCTGACC AGCGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAC GCCCATCTGA AACAGATCTT CCAGGAGGCT 
GACGACATGG TTGCACAAAA GCAGTTTGGA CACCCACAGA TCGAGACCCG TGTCAAAGAA 
GTGTCAGCTC AGTGGGACCA CCTGAAGGAG CTGGCTGCCT TTCGTAAGAA AGACCTGCAG 
GATGCGGAAA ACTTCTTCCA GTTCCAGGGT GATGCAGACG ACCTGAAGGC TTGGCTGCAA 
GACGCTCACC GGCTGCTCTC TGGTGAAGAT GTAGGGCAAG ATGAAGGAGC CACGAGGGCC 
CTGGGAAAGA AGCACAAGGA GTTCCTGGAG GAGCTGGAGG AGAGCCGAGG GGTGATGGAG 
CACTTGGAGC ACCAAGCCCA GGGCTTCCCC GAGGAGTTTC GAGATTCCCC AGATGTAACA 
AACCGACTCC AGGCACTCAG GAAGCTCTAC CAGCAGGTAC TGACCCAGGC AGAATTGCGT 
GGACACAAGC TGCAGGAAGC CCTGGACTTG TACACCGTGT TTGGGGAGTC AGATGCCTGT 
GAGCTGTGGA TGACGGAGAA GGGGAAGTGG CTGGACCAGA TGGATATCCC AAACACGCTG 
GAGGATCTGG AGGTGGTGCA GCACAGGTTT GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGGCGCAGA TTGATGGAGT GAACCTTGCC GCCAACAACC TGGTAGAGAG TGGCCACCCA 
CGAAGTGGGG AGGTGAAGCA ATATCAGGAC CGCCTGAACA AGAGGTGGCA GGCCTTCCAG 
GCAGTGGTGT CAGAGCAGAG AGAGGCTGTG GACTCAGCTC TCCGAGTAAA CAATTACTGT 
GTAGACTGTG AGGAGACCAG CAAGTGGATC ATGGACAAGA CGAAGGTTGT GGAGTCAACC 
AAGGACTTGG GACAGGACCT GGCAGGTGTA ATTGCCATCC AGCGGAAGTT GTCAGGGTTG 
GAGCGGGATG TATTGGCCAT CCGGGATCGT GTGAGTGCCC TGGAACGGGA GTCCCAGTAC 
TTGATGGAGT CACACCCTGA GCAGAAAGAG GACATTGGCC AGCGGCAAGC AGATGTTGAG 
AAGCTGTGGA AGGGCCTGCA AGATGCCTTG CAAGGTCAGG AGCTCTCCTT AGGCGAGGCC 
AGCAAGCTGC AGGCTTTCCT GCAAGATCTG GATGACTTCA AGGCCTGGCT GTCCATGGCC 
CAAAAGGCAG TGGCCTCCGA GGACATGCCT GAGTCACTCC CAGAGGCGGA GCAGCTCCTA 
CAGCAGCATG CAGCCATTAA GGAAGAGATT GATGCGCACA GAGACGATTA CCATAGGGTC 
AAGGCCTCTG GGGAGAAAGT GATCGAAGGC CAGACCGACC CAGATTACCA GCTTCTGGGC 
CAGCGGTTAG AGGGCCTAGA TACTGACTGG GATGCTCTAC GCCGGATGTG GGAGAGCCGA 
GGGAACACGC TCACCCAGTG CCTGGGCTTT CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCTCC GGACTCCCTA 
GCGGCAGCAG AGGCGGGCAT CCGAAAGTTT GAGGATTTCC TAGTATCTAT GGAAAACAAC 
CGAGACAAGA TCCTGAGTCC TGTGGACTCT GGCAACAAGC TGGTGGCCGA GGGCAACCTG 
TATTCGAACA AGATCATGGA GAAGGTGCAG CTGATTGAAG ACAGGCACAA GAAGAACAAT 
GAGAAGGCCC AGGAGGCTAC AGTTCTCCTA AAAGACAACC TGGAGTTGCA GAACTTCCTC 
CAGAACTGCA AGGAGCTCAC TCTCTGGATC AATGACAAGC TGCTGACGTC TCCAGACGTC 
TCCTATGATG AAGCTCGCAA CCTTCACAAC AAATGGATGA AACACCAGGC GTTTATGGCA 
GAACTGGCTT CACACCAAGG GTGGCTTGAG AACATTGACG CAGAGGGGAG GCAGCTGATG 
GCAGAGAAGC CCCAGTTCAA GGATGTGGTA TCAGAGAGGC TGGAAGCCCT GCACAAGCTC 
TGGGAGGAGC TGCAGTCCAC CGCCAAGGCG AAGGCAGAAC AACTCTCAGC CGCGCGGAGC 
TCTGACCTGC GCCTGCAGAC CCACGCCGAC CTTAACAAAT GGATCGGTGC CATGGAGGAC 
CAGCTGCGAT CAGACGACCT GGGCAAGGAT CTCACCACTG TCAACCGGAT GTTGGCTAAG 
TTGAAGCGAG TGGAGGAACA AGTGAATTTG CGGAAGGAGG AGCTGGAGGA ACTCTTTGCC 
GACGCGCCCT CGCTAGGAGC GGAGGCAGGA GACACGGACA TGAGCATCGA AAAGCGGTTC 
CTAGACCTTC TAGAACCTCT GGGGAGGAGG AAGAAGCAGC TGGAATTGTC CAAAGCCAAG 
CTACAAATCA GCCGGGACTT AGAGGATGAG ACGCTCTGGG TGGAAGAGAG GCTGCCACTG 
GCTCAGTCAG CTGACTATGG CACTAATCTG CAAACTGTGC AGCTGTTCAT GAAGAAGAAC 
CAGACCCTGC AGAATGAGAT TCTCGGCCAT GCGCCACGGG TGGAGGATGT GCTACGTCGA 
GGGCAGGAGC TGGTGAAGGC AGCCGAGATC GACTGCCAAG ACATCGAGGA GCGCCTGGGG 
CATCTGCAGA GCTCGTGGGA CACGCTGCGG GAGGCAGCAG CTGGCCGGCT GCAGCGCCTG 
CGGGATGCTC ACGAGGCACA GCAGTACTAC CTGGATGCAG GCGAGGCCGA AGCCTGGATC 
AGCGAGCAGG AGCTATATGT GTTCTCTGAT GAACCCCCTA AGGATGAAGA GGGAGCCATT 
GTGATGCTCA AACGGCACCT GCGGCAGCAG CGCACTGTGG AGGAGTATGG ACGGAATATC 
AAACAGCTGG CTGGCCGTGC CCAGAGCCTG CTATCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TCCAGGGGCA GGTGGATAAG CAGTACGCGG GGCTGAAGGA CATGGCTGAA 
GAGCGGAGGC GGAGGCTGGA GAACATGTAC CACCTGTTCC AGCTGAAGAG AGAGGCTGAC 
GACTTAGAGC AGTGGATTAC GGAAAAAGAG ATGGTGGCCT CGTCCCAAGA AATGGGGCAG 
GATTTTGACC ACGTGACTAT GCTACGTGAC AAGTTCCGAG ACTTTGCCCG GGAGACCGGG 
GCCATCGGGC AGGAGCGAGT GGACAACGTG AATACGATCA TTGAGCGGCT CATCGATGCG 
GGCCACAGCG AGGCGGCCAC CATCGCTGAG TGGAAGGATG GACTCAATGA CATGTGGGCG 
GACCTGCTGG AGCTCATTGA CACCCGCATG CAGCTGCTGG CTGCCTCCTA CGACCTGCAC 
CGCTATTTCT ACACAGGCAC CGAGATCCTG GGCCTCATAG ATGAGAAGCA CAGAGAGCTG 
CCGGAGGACG TGGGGCTGGA CGCCAGCACT GCTGAGTCCT TCCACCGGGT CCACACTGCC 
TTCGAGCGGG AACTCCACCT CCTAGGAGTG CAGGTACAGC AGTTCCAGGA CGTGGCCACC 
CGACTGCAGA CAGCGTATGC CGGGGAGAAG GCAGATGCTA TCCAGAGCAA AGAGCAGGAG 
GTGTCAGCGG CCTGGCAGGC ACTGCTCGAT GCGTGTGCCG GGCGCCGAGC TCAGCTGGTG 
GACACAGCAG ACAAGTTCCG CTTCTTCAGC ATGGTGCGAG ACCTCCTCTC CTGGATGGAG 
AGCATCATCA GGCAGATCGA GACCCAGGAG AGACCCAGGG ATGTTTCCTC TGTGGAATTG 
CTCTTGAAGT ATCATCAGGG CATCAAGGCA GAGATCAATA CCAGGGCCAA GAACTTCAGC 
ACCTGCCTAG AGCTTGGGGA GTCCCTGCTA CAGAGGCAAC ACCAGGCCTC GGACGAGATC 
CGGGAGAAAT TGCAGCAAGT GATATCCAGG AGGCAGGAGA TGAATGACAA GTGGGAGGCC 
CGCTCTGACA GGCTCCACAT GCTTCTGGAA GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCCGAGGCAT GGTTGATTGC CCAGGAGCCC TACCTGGCCA GCCGGGACTT TGGACATACA 
GTGGACAGCG TAGAAAAGCT TATCAAGAGG CACGAGGCGT TTGAGAAGTC CACAGCCAGC 
TGGGCAGAGC GCTTTGCCGC CCTGGAGAAG CCCACCACGC TTGAGCTTAA GGAACGCCAG 
ACTCCAGAAA GACCCACAGA GGAACCTGGG CCTCAAGAGG AGGAAGGCGA GACAGCTGGC 
GAGGCCCCCC AGGTCCACCA TGCGGCCACA GAGAGAACAT CTCCGGGGGA AGAAAGAGGA 
CCATGGCCTC AGGACCTGCA ACCACCACCC CTGCCAGGAC ACCACAAAGA CGAGCAGGAG 
AAATCCGTTG GGGATGAGAG GCCTGCCACA GAGCCCCTCT TTAAAGTCCT GGACACGCCC 
CTGAGTGAGG GTGACGAGCC CACGACCCTG CCAGCCCAGC GAGACCTCGG GCACACTGTG 
CAGATGGAGG GCTACCTGGG CCGAAAGCAC GACCTAGAGG GGCCCAACAA GAAGGCCTCT 
AACAGGTCCT GGAACAACCT GTACTGTGTG CTCAGGAATA GTCAGCTGAC CTTCTACAAG 
GATGCTAAGA ACCTGGCCCT TGGGGTGCCC TACCATGGAG AGGAGCCCCT GGCCCTGAGG 
CACGCCATCT GTGAGATTGC TGTCAACTAT AAGAAGAAGA AGCATGTGTT TAAGCTGAGG 
TTAAGCAATG GCAGCGAGTG GCTCTTCCAT GGCAAGGATG AGGAGGAGAT GCTGATGTGG 
CTACAGAGCA TGAGCACGGC CATCAACGAG TCCCAGAGCA TCCGTGTCAA GGCTCAGAGT 
CTGCCTCTGC CTTCCCTGGC TGGTCCTGAT GCCAGCGTTG GCAAGAAGGA AAAGGAGAAA 
AGGTTTAGCT TCTTCCCCAA AAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038703.3
CDS	668..7657
Translation	MTSATEFENVGNQPPFSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRISDLYKDLRDGRMLIKLLEVLSGEMLPRPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFDVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTISVLNSRKFANSLSGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYQRELALRSELIRQEKLEQLARRFDRKAAMRETWLNENQRLVTQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVKALEDLAQELEKENYHDQKRIIARKDNILRLWSYLQELLRSRRQRLEATLALQKLFQDMLHSIDWMDEIKAHILSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLQFAEGKGYQPCDPQVIQDRVSHLEQCFSELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLKQIFQEADDMVAQKQFGHPQIETRVKEVSAQWDHLKELAAFRKKDLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKEFLEELEESRGVMEHLEHQAQGFPEEFRDSPDVTNRLQALRKLYQQVLTQAELRGHKLQEALDLYTVFGESDACELWMTEKGKWLDQMDIPNTLEDLEVVQHRFDILDQEMKTLMAQIDGVNLAANNLVESGHPRSGEVKQYQDRLNKRWQAFQAVVSEQREAVDSALRVNNYCVDCEETSKWIMDKTKVVESTKDLGQDLAGVIAIQRKLSGLERDVLAIRDRVSALERESQYLMESHPEQKEDIGQRQADVEKLWKGLQDALQGQELSLGEASKLQAFLQDLDDFKAWLSMAQKAVASEDMPESLPEAEQLLQQHAAIKEEIDAHRDDYHRVKASGEKVIEGQTDPDYQLLGQRLEGLDTDWDALRRMWESRGNTLTQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLAAAEAGIRKFEDFLVSMENNRDKILSPVDSGNKLVAEGNLYSNKIMEKVQLIEDRHKKNNEKAQEATVLLKDNLELQNFLQNCKELTLWINDKLLTSPDVSYDEARNLHNKWMKHQAFMAELASHQGWLENIDAEGRQLMAEKPQFKDVVSERLEALHKLWEELQSTAKAKAEQLSAARSSDLRLQTHADLNKWIGAMEDQLRSDDLGKDLTTVNRMLAKLKRVEEQVNLRKEELEELFADAPSLGAEAGDTDMSIEKRFLDLLEPLGRRKKQLELSKAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHAPRVEDVLRRGQELVKAAEIDCQDIEERLGHLQSSWDTLREAAAGRLQRLRDAHEAQQYYLDAGEAEAWISEQELYVFSDEPPKDEEGAIVMLKRHLRQQRTVEEYGRNIKQLAGRAQSLLSAGHPEGEQIIRLQGQVDKQYAGLKDMAEERRRRLENMYHLFQLKREADDLEQWITEKEMVASSQEMGQDFDHVTMLRDKFRDFARETGAIGQERVDNVNTIIERLIDAGHSEAATIAEWKDGLNDMWADLLELIDTRMQLLAASYDLHRYFYTGTEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKADAIQSKEQEVSAAWQALLDACAGRRAQLVDTADKFRFFSMVRDLLSWMESIIRQIETQERPRDVSSVELLLKYHQGIKAEINTRAKNFSTCLELGESLLQRQHQASDEIREKLQQVISRRQEMNDKWEARSDRLHMLLEVCQFSRDASVAEAWLIAQEPYLASRDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQTPERPTEEPGPQEEEGETAGEAPQVHHAATERTSPGEERGPWPQDLQPPPLPGHHKDEQEKSVGDERPATEPLFKVLDTPLSEGDEPTTLPAQRDLGHTVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSQLTFYKDAKNLALGVPYHGEEPLALRHAICEIAVNYKKKKHVFKLRLSNGSEWLFHGKDEEEMLMWLQSMSTAINESQSIRVKAQSLPLPSLAGPDASVGKKEKEKRFSFFPKKK

Target ORF information:

RefSeq Version	NM_013675.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, erythrocytic (Sptb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013675.3

ORF Insert Sequence:

ATGACATCGG CCACGGAGTT TGAAAACGTG GGCAACCAGC CACCATTCAG CCGGATTAAC 
GCCCGCTGGG ATGCCCCCGA TGACGAACTG GATAATGACA ACAGCTCAGC CAGGCTCTTT 
GAGAGGTCAC GGATCAAGGC CTTGGCAGAT GAGCGGGAAG TGGTCCAGAA GAAAACCTTT 
ACGAAATGGG TGAACTCCCA CCTGGCTCGA GTGTCCTGCC GCATCAGCGA CCTCTACAAG 
GACCTGCGGG ACGGACGGAT GCTCATCAAG CTGCTGGAGG TGCTCTCTGG AGAGATGCTG 
CCAAGGCCGA CCAAGGGCAA GATGCGCATC CACTGCCTGG AGAACGTGGA CAAAGCCCTG 
CAGTTCCTCA AGGAACAGCG CGTGCACCTG GAAAACATGG GCTCCCACGA CATTGTGGAT 
GGTAACCACC GCCTGGTCCT GGGCCTCATC TGGACCATCA TCCTTCGATT CCAGATTCAG 
GACATTGTTG TCCAAACTCA AGAAGGTCGG GAGACACGCT CAGCCAAGGA TGCTCTGCTC 
CTGTGGTGCC AGATGAAGAC AGCAGGCTAC CCCCATGTCA ATGTAACCAA CTTCACCTCC 
AGCTGGAAGG ATGGCCTGGC CTTCAATGCC CTAATACACA AACACCGGCC TGACCTCATC 
GACTTTGACA AGCTGAAGGA CTCCAATGCC CGGCATAACC TGGAGCATGC TTTTGATGTA 
GCCGAGCGCC AGCTCGGCAT TATCCCACTC CTTGACCCCG AAGATGTCTT CACAGAAAAC 
CCCGATGAGA AATCCATCAT CACCTACGTG GTGGCGTTTT ATCACTACTT CTCCAAGATG 
AAGGTGCTGG CGGTGGAGGG CAAGCGAGTT GGCAAGGTCA TCGACCACGC CATCGAGACC 
GAGAAGATGA TTGAAAAGTA CAGTGGGCTA GCCTCAGACC TGCTCACTTG GATTGAACAA 
ACCATTAGCG TCCTAAACAG CCGCAAGTTT GCCAACTCGT TGAGTGGCGT CCAGCAGCAG 
CTACAGGCCT TCAGCACCTA CCGTACTGTG GAGAAGCCAC CCAAGTTTCA GGAGAAGGGG 
AATCTGGAAG TCCTCCTATT CACTATCCAA TCCCGGATGA GAGCCAACAA TCAGAAGGTG 
TACACGCCCC ACGATGGGAA GCTGGTGTCT GACATCAACA GGGCCTGGGA GAGCCTGGAG 
GAAGCAGAGT ACCAAAGGGA GCTGGCTCTG AGGAGCGAGC TCATTCGGCA GGAGAAGCTG 
GAGCAGCTGG CGCGGCGCTT CGACCGGAAG GCTGCCATGA GAGAGACGTG GCTCAATGAA 
AACCAGCGCC TGGTGACCCA GGATAACTTT GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ACGAAGCCAT TGAGACCGAC ACAGCTGCCT ATGAGGAGCG GGTAAAGGCG 
CTGGAGGACC TGGCCCAGGA GCTGGAGAAG GAGAATTACC ATGACCAGAA GCGTATCATT 
GCCCGGAAGG ACAACATCCT CCGTCTGTGG AGCTACCTGC AGGAGCTGCT GCGATCCCGG 
CGACAGAGGC TGGAGGCCAC CCTGGCACTG CAGAAACTCT TTCAGGACAT GCTGCACAGC 
ATCGATTGGA TGGATGAGAT CAAGGCTCAC ATCTTGTCTG CTGAATTTGG GAAGCACTTG 
TTAGAGGTTG AAGACCTGCT GCAGAAGCAC AAGCTAATGG AGGCCGACAT TGCCATCCAA 
GGGGACAAAG TGAAGGCCAT CACAGCGGCC ACCCTGCAGT TCGCTGAGGG GAAAGGGTAT 
CAACCTTGTG ACCCCCAGGT CATCCAGGAC CGTGTCAGCC ACCTAGAGCA GTGCTTCAGT 
GAACTGAGCA ACATGGCAGC TGGACGGAAG GCTCAGCTGG AGCAGTCTAA GCGACTCTGG 
AAGTTCTTCT GGGAGATGGA TGAGGCCGAA AGCTGGATCA AGGAGAAGGA GCAGATCTAC 
TCCTCCCTGG ACTACGGCAA GGACCTGACC AGCGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTTGAGG ATGAGCTCCG TGGGCTGGAC GCCCATCTGA AACAGATCTT CCAGGAGGCT 
GACGACATGG TTGCACAAAA GCAGTTTGGA CACCCACAGA TCGAGACCCG TGTCAAAGAA 
GTGTCAGCTC AGTGGGACCA CCTGAAGGAG CTGGCTGCCT TTCGTAAGAA AGACCTGCAG 
GATGCGGAAA ACTTCTTCCA GTTCCAGGGT GATGCAGACG ACCTGAAGGC TTGGCTGCAA 
GACGCTCACC GGCTGCTCTC TGGTGAAGAT GTAGGGCAAG ATGAAGGAGC CACGAGGGCC 
CTGGGAAAGA AGCACAAGGA GTTCCTGGAG GAGCTGGAGG AGAGCCGAGG GGTGATGGAG 
CACTTGGAGC ACCAAGCCCA GGGCTTCCCC GAGGAGTTTC GAGATTCCCC AGATGTAACA 
AACCGACTCC AGGCACTCAG GAAGCTCTAC CAGCAGGTAC TGACCCAGGC AGAATTGCGT 
GGACACAAGC TGCAGGAAGC CCTGGACTTG TACACCGTGT TTGGGGAGTC AGATGCCTGT 
GAGCTGTGGA TGACGGAGAA GGGGAAGTGG CTGGACCAGA TGGATATCCC AAACACGCTG 
GAGGATCTGG AGGTGGTGCA GCACAGGTTT GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGGCGCAGA TTGATGGAGT GAACCTTGCC GCCAACAACC TGGTAGAGAG TGGCCACCCA 
CGAAGTGGGG AGGTGAAGCA ATATCAGGAC CGCCTGAACA AGAGGTGGCA GGCCTTCCAG 
GCAGTGGTGT CAGAGCAGAG AGAGGCTGTG GACTCAGCTC TCCGAGTAAA CAATTACTGT 
GTAGACTGTG AGGAGACCAG CAAGTGGATC ATGGACAAGA CGAAGGTTGT GGAGTCAACC 
AAGGACTTGG GACAGGACCT GGCAGGTGTA ATTGCCATCC AGCGGAAGTT GTCAGGGTTG 
GAGCGGGATG TATTGGCCAT CCGGGATCGT GTGAGTGCCC TGGAACGGGA GTCCCAGTAC 
TTGATGGAGT CACACCCTGA GCAGAAAGAG GACATTGGCC AGCGGCAAGC AGATGTTGAG 
AAGCTGTGGA AGGGCCTGCA AGATGCCTTG CAAGGTCAGG AGCTCTCCTT AGGCGAGGCC 
AGCAAGCTGC AGGCTTTCCT GCAAGATCTG GATGACTTCA AGGCCTGGCT GTCCATGGCC 
CAAAAGGCAG TGGCCTCCGA GGACATGCCT GAGTCACTCC CAGAGGCGGA GCAGCTCCTA 
CAGCAGCATG CAGCCATTAA GGAAGAGATT GATGCGCACA GAGACGATTA CCATAGGGTC 
AAGGCCTCTG GGGAGAAAGT GATCGAAGGC CAGACCGACC CAGATTACCA GCTTCTGGGC 
CAGCGGTTAG AGGGCCTAGA TACTGACTGG GATGCTCTAC GCCGGATGTG GGAGAGCCGA 
GGGAACACGC TCACCCAGTG CCTGGGCTTT CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACT TGGAGCCTCC GGACTCCCTA 
GCGGCAGCAG AGGCGGGCAT CCGAAAGTTT GAGGATTTCC TAGTATCTAT GGAAAACAAC 
CGAGACAAGA TCCTGAGTCC TGTGGACTCT GGCAACAAGC TGGTGGCCGA GGGCAACCTG 
TATTCGAACA AGATCATGGA GAAGGTGCAG CTGATTGAAG ACAGGCACAA GAAGAACAAT 
GAGAAGGCCC AGGAGGCTAC AGTTCTCCTA AAAGACAACC TGGAGTTGCA GAACTTCCTC 
CAGAACTGCA AGGAGCTCAC TCTCTGGATC AATGACAAGC TGCTGACGTC TCCAGACGTC 
TCCTATGATG AAGCTCGCAA CCTTCACAAC AAATGGATGA AACACCAGGC GTTTATGGCA 
GAACTGGCTT CACACCAAGG GTGGCTTGAG AACATTGACG CAGAGGGGAG GCAGCTGATG 
GCAGAGAAGC CCCAGTTCAA GGATGTGGTA TCAGAGAGGC TGGAAGCCCT GCACAAGCTC 
TGGGAGGAGC TGCAGTCCAC CGCCAAGGCG AAGGCAGAAC AACTCTCAGC CGCGCGGAGC 
TCTGACCTGC GCCTGCAGAC CCACGCCGAC CTTAACAAAT GGATCGGTGC CATGGAGGAC 
CAGCTGCGAT CAGACGACCT GGGCAAGGAT CTCACCACTG TCAACCGGAT GTTGGCTAAG 
TTGAAGCGAG TGGAGGAACA AGTGAATTTG CGGAAGGAGG AGCTGGAGGA ACTCTTTGCC 
GACGCGCCCT CGCTAGGAGC GGAGGCAGGA GACACGGACA TGAGCATCGA AAAGCGGTTC 
CTAGACCTTC TAGAACCTCT GGGGAGGAGG AAGAAGCAGC TGGAATTGTC CAAAGCCAAG 
CTACAAATCA GCCGGGACTT AGAGGATGAG ACGCTCTGGG TGGAAGAGAG GCTGCCACTG 
GCTCAGTCAG CTGACTATGG CACTAATCTG CAAACTGTGC AGCTGTTCAT GAAGAAGAAC 
CAGACCCTGC AGAATGAGAT TCTCGGCCAT GCGCCACGGG TGGAGGATGT GCTACGTCGA 
GGGCAGGAGC TGGTGAAGGC AGCCGAGATC GACTGCCAAG ACATCGAGGA GCGCCTGGGG 
CATCTGCAGA GCTCGTGGGA CACGCTGCGG GAGGCAGCAG CTGGCCGGCT GCAGCGCCTG 
CGGGATGCTC ACGAGGCACA GCAGTACTAC CTGGATGCAG GCGAGGCCGA AGCCTGGATC 
AGCGAGCAGG AGCTATATGT GTTCTCTGAT GAACCCCCTA AGGATGAAGA GGGAGCCATT 
GTGATGCTCA AACGGCACCT GCGGCAGCAG CGCACTGTGG AGGAGTATGG ACGGAATATC 
AAACAGCTGG CTGGCCGTGC CCAGAGCCTG CTATCTGCAG GCCACCCTGA GGGGGAACAG 
ATCATCAGAC TCCAGGGGCA GGTGGATAAG CAGTACGCGG GGCTGAAGGA CATGGCTGAA 
GAGCGGAGGC GGAGGCTGGA GAACATGTAC CACCTGTTCC AGCTGAAGAG AGAGGCTGAC 
GACTTAGAGC AGTGGATTAC GGAAAAAGAG ATGGTGGCCT CGTCCCAAGA AATGGGGCAG 
GATTTTGACC ACGTGACTAT GCTACGTGAC AAGTTCCGAG ACTTTGCCCG GGAGACCGGG 
GCCATCGGGC AGGAGCGAGT GGACAACGTG AATACGATCA TTGAGCGGCT CATCGATGCG 
GGCCACAGCG AGGCGGCCAC CATCGCTGAG TGGAAGGATG GACTCAATGA CATGTGGGCG 
GACCTGCTGG AGCTCATTGA CACCCGCATG CAGCTGCTGG CTGCCTCCTA CGACCTGCAC 
CGCTATTTCT ACACAGGCAC CGAGATCCTG GGCCTCATAG ATGAGAAGCA CAGAGAGCTG 
CCGGAGGACG TGGGGCTGGA CGCCAGCACT GCTGAGTCCT TCCACCGGGT CCACACTGCC 
TTCGAGCGGG AACTCCACCT CCTAGGAGTG CAGGTACAGC AGTTCCAGGA CGTGGCCACC 
CGACTGCAGA CAGCGTATGC CGGGGAGAAG GCAGATGCTA TCCAGAGCAA AGAGCAGGAG 
GTGTCAGCGG CCTGGCAGGC ACTGCTCGAT GCGTGTGCCG GGCGCCGAGC TCAGCTGGTG 
GACACAGCAG ACAAGTTCCG CTTCTTCAGC ATGGTGCGAG ACCTCCTCTC CTGGATGGAG 
AGCATCATCA GGCAGATCGA GACCCAGGAG AGACCCAGGG ATGTTTCCTC TGTGGAATTG 
CTCTTGAAGT ATCATCAGGG CATCAAGGCA GAGATCAATA CCAGGGCCAA GAACTTCAGC 
ACCTGCCTAG AGCTTGGGGA GTCCCTGCTA CAGAGGCAAC ACCAGGCCTC GGACGAGATC 
CGGGAGAAAT TGCAGCAAGT GATATCCAGG AGGCAGGAGA TGAATGACAA GTGGGAGGCC 
CGCTCTGACA GGCTCCACAT GCTTCTGGAA GTGTGCCAGT TCTCGAGGGA TGCCTCTGTG 
GCCGAGGCAT GGTTGATTGC CCAGGAGCCC TACCTGGCCA GCCGGGACTT TGGACATACA 
GTGGACAGCG TAGAAAAGCT TATCAAGAGG CACGAGGCGT TTGAGAAGTC CACAGCCAGC 
TGGGCAGAGC GCTTTGCCGC CCTGGAGAAG CCCACCACGC TTGAGCTTAA GGAACGCCAG 
ACTCCAGAAA GACCCACAGA GGAACCTGGG CCTCAAGAGG AGGAAGGCGA GACAGCTGGC 
GAGGCCCCCC AGGTCCACCA TGCGGCCACA GAGAGAACAT CTCCGGGGGA AGAAAGAGGA 
CCATGGCCTC AGGACCTGCA ACCACCACCC CTGCCAGGAC ACCACAAAGA CGAGCAGGAG 
AAATCCGTTG GGGATGAGAG GCCTGCCACA GAGCCCCTCT TTAAAGTCCT GGACACGCCC 
CTGAGTGAGG GTGACGAGCC CACGACCCTG CCAGCCCAGC GAGACCTCGG GCACACTGTG 
CAGATGGAGG GCTACCTGGG CCGAAAGCAC GACCTAGAGG GGCCCAACAA GAAGGCCTCT 
AACAGGTCCT GGAACAACCT GTACTGTGTG CTCAGGAATA GTCAGCTGAC CTTCTACAAG 
GATGCTAAGA ACCTGGCCCT TGGGGTGCCC TACCATGGAG AGGAGCCCCT GGCCCTGAGG 
CACGCCATCT GTGAGATTGC TGTCAACTAT AAGAAGAAGA AGCATGTGTT TAAGCTGAGG 
TTAAGCAATG GCAGCGAGTG GCTCTTCCAT GGCAAGGATG AGGAGGAGAT GCTGATGTGG 
CTACAGAGCA TGAGCACGGC CATCAACGAG TCCCAGAGCA TCCGTGTCAA GGCTCAGAGT 
CTGCCTCTGC CTTCCCTGGC TGGTCCTGAT GCCAGCGTTG GCAAGAAGGA AAAGGAGAAA 
AGGTTTAGCT TCTTCCCCAA AAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Switching of ?-Catenin From Epithelial to Neuronal Type During Lens Epithelial Cell Differentiation.
Investigative ophthalmology & visual science58(9)3445-3455(2017 Jul)
Maddala R,Rao PV

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.
Scientific reports7(1)5164(2017 Jul)
Huang YS,Delgadillo LF,Cyr KH,Kingsley PD,An X,McGrath KE,Mohandas N,Conboy JG,Waugh RE,Wan J,Palis J

A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice.
Scientific reports637197(2016 Nov)
Huang HM,Bauer DC,Lelliott PM,Greth A,McMorran BJ,Foote SJ,Burgio G

Cell organization, growth, and neural and cardiac development require ?II-spectrin.
Journal of cell science124(Pt 23)3956-66(2011 Dec)
Stankewich MC,Cianci CD,Stabach PR,Ji L,Nath A,Morrow JS

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Sptb cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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