tcaf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following tcaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tcaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa12930 XM_004915986.4
Latest version!
Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa12931 XM_031899109.1
Latest version!
Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa12931 XM_002934560.5
Latest version!
Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa12931 XM_031899108.1
Latest version!
Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OXa12930 XM_004915986.3 Xenopus tropicalis TRPM8 channel-associated factor 1 (tcaf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa12931 XM_002934560.4 Xenopus tropicalis TRPM8 channel-associated factor 1 (tcaf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa12930
    Clone ID Related Accession (Same CDS sequence) XM_004915986.4 , XM_004915986.3
    Accession Version XM_004915986.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915986.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATTTTGG AAGCCTCCCA TAAGACTCAA TGGCACTTCA ACCTCGCCCA AGGAAAATTT 
    GGAATTATGG AGCTTTTTGA AGATTACAAA GCACTCACCA AAGATGTTGG TTCTCTAGAT
    TTCTCTGGAA GTTCTGTTCC ATGCAAGCTG CTAATAACAG GAGACATGGC ATTCCCTGTC
    CTGGTCACTC CTAGAAAGGA TGTGCTCATT GCTGCCTCTT ATTATGGAAA GGGGAAAATG
    GTGGTCATTG CTCATGAGAA TTATCTGAAT ATGAAAGAGT TTATGGTTTT CTTAAGGAAT
    TCTGTGACCT GGCTGAAACC AAACCCAAAA GCAGAAATTG GTGTAGATGG CAGCCTAACT
    CTACTAGCAG AGACACTTAC TGGCTCTGGC TGTCAAGTCC AAACCAGTTT AAGCCCTGCT
    GAGGGCTTGG GAGTGTTCTG TACAAGTGGA TATGATGATA GCCAGGCTCA GCAGATCATC
    TCATTTGTGC GAGAGGGGGG AGGCCTGCTG ATTGGGGCTC AGGCTTGGCA CTGGTCCTAC
    AGTCACAAGC AGGAAAATGT TCTCCACAAT TTTCCTGGAA ACAAAATAAC ATCAGTTTCT
    GGGGTGTACT TCACTGATAA TTATGGAGAA AAAGGAACAT TCTGTGTGAC TGATAATATG
    CCATGGACTC CATTATACAC AGACTTTGAC TTTTCAGCAG ATATGAAACA CCTTTTAAAT
    GGTGTATCGC AGCTCGATAT CAGCGGATAC AGCCTCCCCT CAGATCTTCT CATCTATGGG
    GCACTTGCAT TTCCAGTTGG TGTTGCTGAT AAGAAAGAAT GTTTTCTGGG TGCTGCACAC
    TATGGAAAAG GGCGTGTGGT TGTAGCATCA CATGACACCT ACATGTATAA GCCAGAGCTG
    AAAACCTTTA TCCTAAATAC AATATCATGG CTAGACACTG GAAAGAAAAA CAAAATTGGT
    ATTCATAAAG ACTTGGAAAA CCTTGCACAA TTGTTAGAGA AGGAAGGCAT TTTATGTACA
    ATATCCAGCT TTAGCCCTGA GCTGAGTGTA TATTGCTGTC CATGGTACAC CGAGGCTGAA
    GAATGTGCTA TCCATCAATT TGTTGCAGAA GGGGGAGGCC TATTTATTGG TGGCCAGTCC
    TGGAACTGGG CCTCCAAAAA CTCAGATCTA AATGTTGTTG CTGATTTCCC AGGCAACAAA
    ATACTGAACA AATTTGGCAT TAGCATCTTG GACTGGAGCA CCCCTCAAGG CAATTATAAA
    GCAGTCAATC CTGATGAAGC CACTAACCAG TATCGGTTCC CAAAGGCACT TTCACAGCTG
    CAAAACGAAC TAAAAAGTGA ACATGATCTG AAACCTCCCT TTAGCACCTG GATGTTCAAA
    CTAAGACGAG ATATCACCTA TTTCATGAGG CTCCCCGCCA GTCCTATCAT TTGCGCCATA
    CAGCGCCAGT TTATAGATAT GATGCAGACC TGTGAGCTAC CCAAGCTCAA TGGAAAAAAC
    TGTGTGAGCA GTTGCTCGAA AGAAGCTTTA ATCCTATCTT TGGCCCATGA AGCTTGCAAT
    GTGAACCAGG AGTGCAATGA GAAAAATATT ATGGAGAATG AGCCTGCAGT CACAATAGAA
    ATTGATGCCA CAAACCCAGG TTATGATGCA TGGAGAAGCA CCGGGCTCTA TTTGTCTCCC
    AAAAAGACAG CAGTGCTTGT GTTTCCTGCC TCAGCTGTTC AACAGGGTCT TCAGGTCCAG
    GTTGGCTGTC ACTCAGATGA TTTGGGCTCT AAAGATGAGT TTTATCGTGC GCCGATTGTT
    GTGCGGAAAA TTTCTGTTCA TAGAGAGAAG GTCCAGGTTT CCTGTTATTG GGGAGGACTC
    CTTTACATTA TTGTGAAAGA GAGGAGCAAC TTGGGTACAA TCCCAGTTCA AGTGTATGGA
    GCAGAACCAG CCCCAACCTA TATAAAAGGT AAGACCAGTC TTGACTCCTG GTTGCAATCA
    CTCCGTGACT TGCCTCCTCC ATGGGCTGAA TTGATTACAG AAAACATTAT CCTGACTGTA
    CCATCAGATG CTATCCGCTC ACTAAATGAT CCAGAAGAAC TACTTGGGTT TTGGGACAGA
    ATGATGGTAG CTATTACTGA GCTTGCTGCC ATACCGACAA CATTTTCTCG ACCAGAAAGA
    ATCGTAGCAG ATGTTCAGAT CTCAGCTGGT TGGATGCATG CTGGATACCC AGTCATGGTC
    CACTTAGCAT CAGTCAAATA TCTGACTAAC CTTAGCTGTA TGCAAAAGGA AGGTCTCTGG
    GGACCAATCC ATGAGCTTGG GCACAATCAA CAGCAAAAAG TTTGGGAGTT CCCCCCACAC
    ACTACAGAAG CCACTTGTAA CCTTTGGTCT GTATTTGTGA ATGAAACAGT TGTAGGAGTG
    CCCCGGGATA AAGCTAACTC TGCTCTCGAA CCAGCAAGCA GGGCTGCCTG TATTGAGGCA
    TATTTGAAAA CTGGACCCAA GTTAGAAAAT TGGAATGTAT GGACTGCACT GGAGACCTAT
    CTACAGTTGC AAGAAGGCTT TGGCTGGGAA CCATTCAAGC AGTTATTCAA AGACTACCAG
    AGCATGTCGG GCATCAGTAA TGACAACAAA TCAAAGATGA ACCTGTGGGC AGAGAAGTTC
    TCTGAGGCTG TGCAAACCAA TCTCATTCCA TTCTTTGAGG CCTGGGGATG GCCCATTGAG
    GAAGAAACTC GCAGTAAATT ATCTGCATTA CCTGTATGGG AGAAGGATCC CATGAAGCCA
    TATCTTAGTG CAATGAAGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916043.1
    CDS333..3116
    Translation

    Target ORF information:

    RefSeq Version XM_004915986.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor 1 (tcaf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915986.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATTTTGG AAGCCTCCCA TAAGACTCAA TGGCACTTCA ACCTCGCCCA AGGAAAATTT 
    GGAATTATGG AGCTTTTTGA AGATTACAAA GCACTCACCA AAGATGTTGG TTCTCTAGAT
    TTCTCTGGAA GTTCTGTTCC ATGCAAGCTG CTAATAACAG GAGACATGGC ATTCCCTGTC
    CTGGTCACTC CTAGAAAGGA TGTGCTCATT GCTGCCTCTT ATTATGGAAA GGGGAAAATG
    GTGGTCATTG CTCATGAGAA TTATCTGAAT ATGAAAGAGT TTATGGTTTT CTTAAGGAAT
    TCTGTGACCT GGCTGAAACC AAACCCAAAA GCAGAAATTG GTGTAGATGG CAGCCTAACT
    CTACTAGCAG AGACACTTAC TGGCTCTGGC TGTCAAGTCC AAACCAGTTT AAGCCCTGCT
    GAGGGCTTGG GAGTGTTCTG TACAAGTGGA TATGATGATA GCCAGGCTCA GCAGATCATC
    TCATTTGTGC GAGAGGGGGG AGGCCTGCTG ATTGGGGCTC AGGCTTGGCA CTGGTCCTAC
    AGTCACAAGC AGGAAAATGT TCTCCACAAT TTTCCTGGAA ACAAAATAAC ATCAGTTTCT
    GGGGTGTACT TCACTGATAA TTATGGAGAA AAAGGAACAT TCTGTGTGAC TGATAATATG
    CCATGGACTC CATTATACAC AGACTTTGAC TTTTCAGCAG ATATGAAACA CCTTTTAAAT
    GGTGTATCGC AGCTCGATAT CAGCGGATAC AGCCTCCCCT CAGATCTTCT CATCTATGGG
    GCACTTGCAT TTCCAGTTGG TGTTGCTGAT AAGAAAGAAT GTTTTCTGGG TGCTGCACAC
    TATGGAAAAG GGCGTGTGGT TGTAGCATCA CATGACACCT ACATGTATAA GCCAGAGCTG
    AAAACCTTTA TCCTAAATAC AATATCATGG CTAGACACTG GAAAGAAAAA CAAAATTGGT
    ATTCATAAAG ACTTGGAAAA CCTTGCACAA TTGTTAGAGA AGGAAGGCAT TTTATGTACA
    ATATCCAGCT TTAGCCCTGA GCTGAGTGTA TATTGCTGTC CATGGTACAC CGAGGCTGAA
    GAATGTGCTA TCCATCAATT TGTTGCAGAA GGGGGAGGCC TATTTATTGG TGGCCAGTCC
    TGGAACTGGG CCTCCAAAAA CTCAGATCTA AATGTTGTTG CTGATTTCCC AGGCAACAAA
    ATACTGAACA AATTTGGCAT TAGCATCTTG GACTGGAGCA CCCCTCAAGG CAATTATAAA
    GCAGTCAATC CTGATGAAGC CACTAACCAG TATCGGTTCC CAAAGGCACT TTCACAGCTG
    CAAAACGAAC TAAAAAGTGA ACATGATCTG AAACCTCCCT TTAGCACCTG GATGTTCAAA
    CTAAGACGAG ATATCACCTA TTTCATGAGG CTCCCCGCCA GTCCTATCAT TTGCGCCATA
    CAGCGCCAGT TTATAGATAT GATGCAGACC TGTGAGCTAC CCAAGCTCAA TGGAAAAAAC
    TGTGTGAGCA GTTGCTCGAA AGAAGCTTTA ATCCTATCTT TGGCCCATGA AGCTTGCAAT
    GTGAACCAGG AGTGCAATGA GAAAAATATT ATGGAGAATG AGCCTGCAGT CACAATAGAA
    ATTGATGCCA CAAACCCAGG TTATGATGCA TGGAGAAGCA CCGGGCTCTA TTTGTCTCCC
    AAAAAGACAG CAGTGCTTGT GTTTCCTGCC TCAGCTGTTC AACAGGGTCT TCAGGTCCAG
    GTTGGCTGTC ACTCAGATGA TTTGGGCTCT AAAGATGAGT TTTATCGTGC GCCGATTGTT
    GTGCGGAAAA TTTCTGTTCA TAGAGAGAAG GTCCAGGTTT CCTGTTATTG GGGAGGACTC
    CTTTACATTA TTGTGAAAGA GAGGAGCAAC TTGGGTACAA TCCCAGTTCA AGTGTATGGA
    GCAGAACCAG CCCCAACCTA TATAAAAGGT AAGACCAGTC TTGACTCCTG GTTGCAATCA
    CTCCGTGACT TGCCTCCTCC ATGGGCTGAA TTGATTACAG AAAACATTAT CCTGACTGTA
    CCATCAGATG CTATCCGCTC ACTAAATGAT CCAGAAGAAC TACTTGGGTT TTGGGACAGA
    ATGATGGTAG CTATTACTGA GCTTGCTGCC ATACCGACAA CATTTTCTCG ACCAGAAAGA
    ATCGTAGCAG ATGTTCAGAT CTCAGCTGGT TGGATGCATG CTGGATACCC AGTCATGGTC
    CACTTAGCAT CAGTCAAATA TCTGACTAAC CTTAGCTGTA TGCAAAAGGA AGGTCTCTGG
    GGACCAATCC ATGAGCTTGG GCACAATCAA CAGCAAAAAG TTTGGGAGTT CCCCCCACAC
    ACTACAGAAG CCACTTGTAA CCTTTGGTCT GTATTTGTGA ATGAAACAGT TGTAGGAGTG
    CCCCGGGATA AAGCTAACTC TGCTCTCGAA CCAGCAAGCA GGGCTGCCTG TATTGAGGCA
    TATTTGAAAA CTGGACCCAA GTTAGAAAAT TGGAATGTAT GGACTGCACT GGAGACCTAT
    CTACAGTTGC AAGAAGGCTT TGGCTGGGAA CCATTCAAGC AGTTATTCAA AGACTACCAG
    AGCATGTCGG GCATCAGTAA TGACAACAAA TCAAAGATGA ACCTGTGGGC AGAGAAGTTC
    TCTGAGGCTG TGCAAACCAA TCTCATTCCA TTCTTTGAGG CCTGGGGATG GCCCATTGAG
    GAAGAAACTC GCAGTAAATT ATCTGCATTA CCTGTATGGG AGAAGGATCC CATGAAGCCA
    TATCTTAGTG CAATGAAGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12931
    Clone ID Related Accession (Same CDS sequence) XM_002934560.4 , XM_031899109.1 , XM_031899108.1 , XM_002934560.5
    Accession Version XM_002934560.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934560.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
    GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC
    ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC
    ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG
    ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA
    GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC
    TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT
    GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC
    AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG
    TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG
    ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA
    TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT
    GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA
    AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC
    TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT
    AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC
    AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT
    GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC
    TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG
    AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC
    AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC
    GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA
    CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC
    CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG
    AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC
    CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT
    GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG
    ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC
    TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG
    AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC
    ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA
    CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT
    GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA
    GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG
    GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA
    GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA
    GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA
    ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA
    GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG
    GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG
    AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA
    ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATGA AGTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934606.1
    CDS302..3019
    Translation

    Target ORF information:

    RefSeq Version XM_002934560.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor 1 (tcaf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934560.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
    GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC
    ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC
    ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG
    ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA
    GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC
    TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT
    GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC
    AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG
    TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG
    ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA
    TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT
    GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA
    AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC
    TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT
    AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC
    AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT
    GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC
    TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG
    AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC
    AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC
    GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA
    CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC
    CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG
    AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC
    CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT
    GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG
    ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC
    TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG
    AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC
    ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA
    CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT
    GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA
    GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG
    GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA
    GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA
    GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA
    ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA
    GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG
    GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG
    AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA
    ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATGA AGTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12930
    Clone ID Related Accession (Same CDS sequence) XM_004915986.4 , XM_004915986.3
    Accession Version XM_004915986.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915986.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATTTTGG AAGCCTCCCA TAAGACTCAA TGGCACTTCA ACCTCGCCCA AGGAAAATTT 
    GGAATTATGG AGCTTTTTGA AGATTACAAA GCACTCACCA AAGATGTTGG TTCTCTAGAT
    TTCTCTGGAA GTTCTGTTCC ATGCAAGCTG CTAATAACAG GAGACATGGC ATTCCCTGTC
    CTGGTCACTC CTAGAAAGGA TGTGCTCATT GCTGCCTCTT ATTATGGAAA GGGGAAAATG
    GTGGTCATTG CTCATGAGAA TTATCTGAAT ATGAAAGAGT TTATGGTTTT CTTAAGGAAT
    TCTGTGACCT GGCTGAAACC AAACCCAAAA GCAGAAATTG GTGTAGATGG CAGCCTAACT
    CTACTAGCAG AGACACTTAC TGGCTCTGGC TGTCAAGTCC AAACCAGTTT AAGCCCTGCT
    GAGGGCTTGG GAGTGTTCTG TACAAGTGGA TATGATGATA GCCAGGCTCA GCAGATCATC
    TCATTTGTGC GAGAGGGGGG AGGCCTGCTG ATTGGGGCTC AGGCTTGGCA CTGGTCCTAC
    AGTCACAAGC AGGAAAATGT TCTCCACAAT TTTCCTGGAA ACAAAATAAC ATCAGTTTCT
    GGGGTGTACT TCACTGATAA TTATGGAGAA AAAGGAACAT TCTGTGTGAC TGATAATATG
    CCATGGACTC CATTATACAC AGACTTTGAC TTTTCAGCAG ATATGAAACA CCTTTTAAAT
    GGTGTATCGC AGCTCGATAT CAGCGGATAC AGCCTCCCCT CAGATCTTCT CATCTATGGG
    GCACTTGCAT TTCCAGTTGG TGTTGCTGAT AAGAAAGAAT GTTTTCTGGG TGCTGCACAC
    TATGGAAAAG GGCGTGTGGT TGTAGCATCA CATGACACCT ACATGTATAA GCCAGAGCTG
    AAAACCTTTA TCCTAAATAC AATATCATGG CTAGACACTG GAAAGAAAAA CAAAATTGGT
    ATTCATAAAG ACTTGGAAAA CCTTGCACAA TTGTTAGAGA AGGAAGGCAT TTTATGTACA
    ATATCCAGCT TTAGCCCTGA GCTGAGTGTA TATTGCTGTC CATGGTACAC CGAGGCTGAA
    GAATGTGCTA TCCATCAATT TGTTGCAGAA GGGGGAGGCC TATTTATTGG TGGCCAGTCC
    TGGAACTGGG CCTCCAAAAA CTCAGATCTA AATGTTGTTG CTGATTTCCC AGGCAACAAA
    ATACTGAACA AATTTGGCAT TAGCATCTTG GACTGGAGCA CCCCTCAAGG CAATTATAAA
    GCAGTCAATC CTGATGAAGC CACTAACCAG TATCGGTTCC CAAAGGCACT TTCACAGCTG
    CAAAACGAAC TAAAAAGTGA ACATGATCTG AAACCTCCCT TTAGCACCTG GATGTTCAAA
    CTAAGACGAG ATATCACCTA TTTCATGAGG CTCCCCGCCA GTCCTATCAT TTGCGCCATA
    CAGCGCCAGT TTATAGATAT GATGCAGACC TGTGAGCTAC CCAAGCTCAA TGGAAAAAAC
    TGTGTGAGCA GTTGCTCGAA AGAAGCTTTA ATCCTATCTT TGGCCCATGA AGCTTGCAAT
    GTGAACCAGG AGTGCAATGA GAAAAATATT ATGGAGAATG AGCCTGCAGT CACAATAGAA
    ATTGATGCCA CAAACCCAGG TTATGATGCA TGGAGAAGCA CCGGGCTCTA TTTGTCTCCC
    AAAAAGACAG CAGTGCTTGT GTTTCCTGCC TCAGCTGTTC AACAGGGTCT TCAGGTCCAG
    GTTGGCTGTC ACTCAGATGA TTTGGGCTCT AAAGATGAGT TTTATCGTGC GCCGATTGTT
    GTGCGGAAAA TTTCTGTTCA TAGAGAGAAG GTCCAGGTTT CCTGTTATTG GGGAGGACTC
    CTTTACATTA TTGTGAAAGA GAGGAGCAAC TTGGGTACAA TCCCAGTTCA AGTGTATGGA
    GCAGAACCAG CCCCAACCTA TATAAAAGGT AAGACCAGTC TTGACTCCTG GTTGCAATCA
    CTCCGTGACT TGCCTCCTCC ATGGGCTGAA TTGATTACAG AAAACATTAT CCTGACTGTA
    CCATCAGATG CTATCCGCTC ACTAAATGAT CCAGAAGAAC TACTTGGGTT TTGGGACAGA
    ATGATGGTAG CTATTACTGA GCTTGCTGCC ATACCGACAA CATTTTCTCG ACCAGAAAGA
    ATCGTAGCAG ATGTTCAGAT CTCAGCTGGT TGGATGCATG CTGGATACCC AGTCATGGTC
    CACTTAGCAT CAGTCAAATA TCTGACTAAC CTTAGCTGTA TGCAAAAGGA AGGTCTCTGG
    GGACCAATCC ATGAGCTTGG GCACAATCAA CAGCAAAAAG TTTGGGAGTT CCCCCCACAC
    ACTACAGAAG CCACTTGTAA CCTTTGGTCT GTATTTGTGA ATGAAACAGT TGTAGGAGTG
    CCCCGGGATA AAGCTAACTC TGCTCTCGAA CCAGCAAGCA GGGCTGCCTG TATTGAGGCA
    TATTTGAAAA CTGGACCCAA GTTAGAAAAT TGGAATGTAT GGACTGCACT GGAGACCTAT
    CTACAGTTGC AAGAAGGCTT TGGCTGGGAA CCATTCAAGC AGTTATTCAA AGACTACCAG
    AGCATGTCGG GCATCAGTAA TGACAACAAA TCAAAGATGA ACCTGTGGGC AGAGAAGTTC
    TCTGAGGCTG TGCAAACCAA TCTCATTCCA TTCTTTGAGG CCTGGGGATG GCCCATTGAG
    GAAGAAACTC GCAGTAAATT ATCTGCATTA CCTGTATGGG AGAAGGATCC CATGAAGCCA
    TATCTTAGTG CAATGAAGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916043.1
    CDS391..3174
    Translation

    Target ORF information:

    RefSeq Version XM_004915986.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915986.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATTTTGG AAGCCTCCCA TAAGACTCAA TGGCACTTCA ACCTCGCCCA AGGAAAATTT 
    GGAATTATGG AGCTTTTTGA AGATTACAAA GCACTCACCA AAGATGTTGG TTCTCTAGAT
    TTCTCTGGAA GTTCTGTTCC ATGCAAGCTG CTAATAACAG GAGACATGGC ATTCCCTGTC
    CTGGTCACTC CTAGAAAGGA TGTGCTCATT GCTGCCTCTT ATTATGGAAA GGGGAAAATG
    GTGGTCATTG CTCATGAGAA TTATCTGAAT ATGAAAGAGT TTATGGTTTT CTTAAGGAAT
    TCTGTGACCT GGCTGAAACC AAACCCAAAA GCAGAAATTG GTGTAGATGG CAGCCTAACT
    CTACTAGCAG AGACACTTAC TGGCTCTGGC TGTCAAGTCC AAACCAGTTT AAGCCCTGCT
    GAGGGCTTGG GAGTGTTCTG TACAAGTGGA TATGATGATA GCCAGGCTCA GCAGATCATC
    TCATTTGTGC GAGAGGGGGG AGGCCTGCTG ATTGGGGCTC AGGCTTGGCA CTGGTCCTAC
    AGTCACAAGC AGGAAAATGT TCTCCACAAT TTTCCTGGAA ACAAAATAAC ATCAGTTTCT
    GGGGTGTACT TCACTGATAA TTATGGAGAA AAAGGAACAT TCTGTGTGAC TGATAATATG
    CCATGGACTC CATTATACAC AGACTTTGAC TTTTCAGCAG ATATGAAACA CCTTTTAAAT
    GGTGTATCGC AGCTCGATAT CAGCGGATAC AGCCTCCCCT CAGATCTTCT CATCTATGGG
    GCACTTGCAT TTCCAGTTGG TGTTGCTGAT AAGAAAGAAT GTTTTCTGGG TGCTGCACAC
    TATGGAAAAG GGCGTGTGGT TGTAGCATCA CATGACACCT ACATGTATAA GCCAGAGCTG
    AAAACCTTTA TCCTAAATAC AATATCATGG CTAGACACTG GAAAGAAAAA CAAAATTGGT
    ATTCATAAAG ACTTGGAAAA CCTTGCACAA TTGTTAGAGA AGGAAGGCAT TTTATGTACA
    ATATCCAGCT TTAGCCCTGA GCTGAGTGTA TATTGCTGTC CATGGTACAC CGAGGCTGAA
    GAATGTGCTA TCCATCAATT TGTTGCAGAA GGGGGAGGCC TATTTATTGG TGGCCAGTCC
    TGGAACTGGG CCTCCAAAAA CTCAGATCTA AATGTTGTTG CTGATTTCCC AGGCAACAAA
    ATACTGAACA AATTTGGCAT TAGCATCTTG GACTGGAGCA CCCCTCAAGG CAATTATAAA
    GCAGTCAATC CTGATGAAGC CACTAACCAG TATCGGTTCC CAAAGGCACT TTCACAGCTG
    CAAAACGAAC TAAAAAGTGA ACATGATCTG AAACCTCCCT TTAGCACCTG GATGTTCAAA
    CTAAGACGAG ATATCACCTA TTTCATGAGG CTCCCCGCCA GTCCTATCAT TTGCGCCATA
    CAGCGCCAGT TTATAGATAT GATGCAGACC TGTGAGCTAC CCAAGCTCAA TGGAAAAAAC
    TGTGTGAGCA GTTGCTCGAA AGAAGCTTTA ATCCTATCTT TGGCCCATGA AGCTTGCAAT
    GTGAACCAGG AGTGCAATGA GAAAAATATT ATGGAGAATG AGCCTGCAGT CACAATAGAA
    ATTGATGCCA CAAACCCAGG TTATGATGCA TGGAGAAGCA CCGGGCTCTA TTTGTCTCCC
    AAAAAGACAG CAGTGCTTGT GTTTCCTGCC TCAGCTGTTC AACAGGGTCT TCAGGTCCAG
    GTTGGCTGTC ACTCAGATGA TTTGGGCTCT AAAGATGAGT TTTATCGTGC GCCGATTGTT
    GTGCGGAAAA TTTCTGTTCA TAGAGAGAAG GTCCAGGTTT CCTGTTATTG GGGAGGACTC
    CTTTACATTA TTGTGAAAGA GAGGAGCAAC TTGGGTACAA TCCCAGTTCA AGTGTATGGA
    GCAGAACCAG CCCCAACCTA TATAAAAGGT AAGACCAGTC TTGACTCCTG GTTGCAATCA
    CTCCGTGACT TGCCTCCTCC ATGGGCTGAA TTGATTACAG AAAACATTAT CCTGACTGTA
    CCATCAGATG CTATCCGCTC ACTAAATGAT CCAGAAGAAC TACTTGGGTT TTGGGACAGA
    ATGATGGTAG CTATTACTGA GCTTGCTGCC ATACCGACAA CATTTTCTCG ACCAGAAAGA
    ATCGTAGCAG ATGTTCAGAT CTCAGCTGGT TGGATGCATG CTGGATACCC AGTCATGGTC
    CACTTAGCAT CAGTCAAATA TCTGACTAAC CTTAGCTGTA TGCAAAAGGA AGGTCTCTGG
    GGACCAATCC ATGAGCTTGG GCACAATCAA CAGCAAAAAG TTTGGGAGTT CCCCCCACAC
    ACTACAGAAG CCACTTGTAA CCTTTGGTCT GTATTTGTGA ATGAAACAGT TGTAGGAGTG
    CCCCGGGATA AAGCTAACTC TGCTCTCGAA CCAGCAAGCA GGGCTGCCTG TATTGAGGCA
    TATTTGAAAA CTGGACCCAA GTTAGAAAAT TGGAATGTAT GGACTGCACT GGAGACCTAT
    CTACAGTTGC AAGAAGGCTT TGGCTGGGAA CCATTCAAGC AGTTATTCAA AGACTACCAG
    AGCATGTCGG GCATCAGTAA TGACAACAAA TCAAAGATGA ACCTGTGGGC AGAGAAGTTC
    TCTGAGGCTG TGCAAACCAA TCTCATTCCA TTCTTTGAGG CCTGGGGATG GCCCATTGAG
    GAAGAAACTC GCAGTAAATT ATCTGCATTA CCTGTATGGG AGAAGGATCC CATGAAGCCA
    TATCTTAGTG CAATGAAGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12931
    Clone ID Related Accession (Same CDS sequence) XM_002934560.4 , XM_031899109.1 , XM_031899108.1 , XM_002934560.5
    Accession Version XM_031899109.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
    GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC
    ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC
    ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG
    ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA
    GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC
    TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT
    GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC
    AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG
    TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG
    ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA
    TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT
    GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA
    AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC
    TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT
    AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC
    AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT
    GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC
    TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG
    AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC
    AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC
    GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA
    CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC
    CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG
    AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC
    CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT
    GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG
    ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC
    TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG
    AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC
    ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA
    CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT
    GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA
    GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG
    GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA
    GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA
    GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA
    ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA
    GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG
    GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG
    AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA
    ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATGA AGTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754969.1
    CDS31..2748
    Translation

    Target ORF information:

    RefSeq Version XM_031899109.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899109.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
    GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC
    ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC
    ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG
    ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA
    GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC
    TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT
    GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC
    AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG
    TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG
    ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA
    TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT
    GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA
    AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC
    TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT
    AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC
    AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT
    GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC
    TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG
    AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC
    AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC
    GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA
    CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC
    CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG
    AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC
    CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT
    GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG
    ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC
    TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG
    AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC
    ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA
    CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT
    GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA
    GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG
    GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA
    GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA
    GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA
    ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA
    GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG
    GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG
    AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA
    ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATGA AGTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12931
    Clone ID Related Accession (Same CDS sequence) XM_002934560.4 , XM_031899109.1 , XM_031899108.1 , XM_002934560.5
    Accession Version XM_031899108.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
    GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC
    ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC
    ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG
    ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA
    GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC
    TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT
    GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC
    AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG
    TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG
    ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA
    TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT
    GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA
    AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC
    TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT
    AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC
    AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT
    GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC
    TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG
    AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC
    AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC
    GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA
    CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC
    CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG
    AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC
    CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT
    GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG
    ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC
    TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG
    AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC
    ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA
    CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT
    GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA
    GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG
    GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA
    GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA
    GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA
    ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA
    GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG
    GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG
    AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA
    ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATGA AGTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754968.1
    CDS107..2824
    Translation

    Target ORF information:

    RefSeq Version XM_031899108.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899108.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
    GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC
    ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC
    ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG
    ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA
    GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC
    TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT
    GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC
    AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG
    TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG
    ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA
    TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT
    GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA
    AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC
    TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT
    AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC
    AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT
    GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC
    TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG
    AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC
    AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC
    GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA
    CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC
    CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG
    AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC
    CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT
    GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG
    ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC
    TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG
    AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC
    ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA
    CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT
    GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA
    GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG
    GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA
    GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA
    GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA
    ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA
    GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG
    GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG
    AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA
    ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATGA AGTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12931
    Clone ID Related Accession (Same CDS sequence) XM_002934560.4 , XM_031899109.1 , XM_031899108.1 , XM_002934560.5
    Accession Version XM_002934560.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934560.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
    GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC
    ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC
    ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG
    ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA
    GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC
    TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT
    GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC
    AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG
    TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG
    ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA
    TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT
    GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA
    AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC
    TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT
    AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC
    AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT
    GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC
    TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG
    AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC
    AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC
    GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA
    CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC
    CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG
    AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC
    CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT
    GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG
    ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC
    TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG
    AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC
    ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA
    CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT
    GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA
    GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG
    GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA
    GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA
    GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA
    ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA
    GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG
    GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG
    AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA
    ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATGA AGTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934606.1
    CDS360..3077
    Translation

    Target ORF information:

    RefSeq Version XM_002934560.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934560.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
    GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC
    ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC
    ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG
    ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA
    GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC
    TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT
    GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC
    AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG
    TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG
    ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA
    TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT
    GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA
    AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC
    TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT
    AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC
    AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT
    GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC
    TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG
    AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC
    AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC
    GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA
    CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC
    CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG
    AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC
    CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT
    GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG
    ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC
    TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG
    AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC
    ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA
    CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT
    GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA
    GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG
    GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA
    GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA
    GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA
    ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA
    GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG
    GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG
    AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA
    ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATGA AGTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.