kdm3b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	kdm3b
Entrez Gene ID	100135177
Full Name	lysine (K)-specific demethylase 3B
Synonyms	jmjd1b
General protein information	Preferred Names lysine (K)-specific demethylase 3B Names lysine-specific demethylase 3B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004912889.3	XP_004912946.1	lysine-specific demethylase 3B isoform X2
XM_002936848.4	XP_002936894.1	lysine-specific demethylase 3B isoform X1
XM_004912889.4	XP_004912946.1	lysine-specific demethylase 3B isoform X2
XM_002936848.5	XP_002936894.1	lysine-specific demethylase 3B isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KDM3B	XP_517956.3
Mus musculus (house mouse)	Kdm3b	NP_001074725.1
Danio rerio (zebrafish)	wu:fe01c10	XP_005173195.1
	KDM3B	XP_002804573.1
Gallus gallus (chicken)	KDM3B	XP_004945121.1
Xenopus tropicalis (tropical clawed frog)	kdm3b	XP_002936894.1
Bos taurus (cattle)	KDM3B	XP_003586349.1
Homo sapiens (human)	KDM3B	NP_057688.2
Canis lupus familiaris (dog)	KDM3B	XP_531921.3
Rattus norvegicus (Norway rat)	Kdm3b	XP_006254677.1

Gene Ontology

The following kdm3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kdm3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa07678	XM_002936848.5 Latest version!	Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa07679	XM_004912889.4 Latest version!	Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OXa07679	XM_004912889.3	Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa07678	XM_002936848.4	Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kdm3b ( XM_002936848.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936894.1 Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.

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CloneID	OXa07678
Clone ID Related Accession (Same CDS sequence)	XM_002936848.4 , XM_002936848.5
Accession Version	XM_002936848.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936848.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTCC CGGTTTCCCC GGTGGGGAAG AGGCTTCGGT TTCTATTTGA CGAATCGGCC 
TCTGGGCGGG AGGGGGCAAC CACTGAACCG TCCACTCCGC TGGGGAAGCG GGCCTGGCAG 
GGCGGGACGG TGCGGGGCCT GAAAGGAGCG GTGCCCGAAG AGCTAGCGAT TTATGTGGAG 
TTTGATGAGA AAAGTTACAA GTCAAATTCT TGGGTGCAGG TTCATGGGGA GGATGTGTTG 
GTGATCCTGA TAGAAGGAGC TTTGGTTTGG TCTCCAAGAA AAGAGCCTAT GTTTATCCAA 
GGAAGCAAAT TATGTCCAAC TCATTGGCCA GCTTTAACCT TTACTCCACT GGTGGACAAG 
TTGGGCTTAG GGACTTTAAT TCCAATAGAA TTTCTTTTGG AAGAGGTTCT GCATTTCCTA 
CCAGATGCCA GCTCTCTGAA ACTTTATCAG CAAATGGGAG CAGACAGTCA TAAACTGGTT 
TTGAAAGAGC AGCCTTTGTT AAAGGATGCA GTGACTTCTG TGATCAATTC AAGGAAAACA 
CAAGATCTCA TTTTAAGAGG TATTTGCAGT ATTCAAGGCC AGGAAGTTAA GGTTTATCAA 
CCAGATACAG AAGTGCCATG GTCTTGTGGA GTTGTGACCC ATCAGGATCT TATTACACGC 
ATGATAAATG TATCAATGAA TGAGGGAAAT GAGATCAAGT CTATTGATCC AAAATTAATT 
CATGTGGTGC TTACTGACGA CACTTTAATA CAACCTGAGG AGGCTACAAA AGCAGGTAAA 
GCATCTAAAT CAAAGAAAAA AAAGGAGAAT ACAGATGCTC GCAGAAGAAA ATTACCTTCA 
GATGCTGAAA ATGATTCTGC TGGAAAAAAG CAGAAAGGCG ATAAGAAAGA TGTGGACAGT 
AATGGAAATG ATGGCAGTGA AGCTAATAGA GGAGTTTGGA AAGCAGGCAG CACTGGAGAA 
GCTACACCAG ATTCAAGATC TAAGCAAATG TTACACTTTG TTCCTCAAAT TAATCGCAAT 
ATCCGATTTG CAACCTACAC CAAAGAGAAT GGACGCACAT TAGTGGTTCA AGATCAGCCT 
GTAAATGATG GAACCTCATT AGCTTTTCCA ACTTTTTCTG GAATGACTAA AGGTATATTG 
TCTGGTGAAA GTGTTGGGAA CTCTGAGAGT GTAACTTCGA GTGGTACTAC TCCTACCACA 
GTACGAATCT CTGATACTAG TCTCTCTGCT TTGACTGGAG AAGAAAAGAA GCCAAACTGG 
TCCCTTGGAG AGAACCTCAG AAAATCACTG CTGACCAAGA GTGGATTTGG CCCATCACTC 
TCTCAGCCAT TATTTGGGAC TGATAAAAAC CTATCTAATG GTGCACTTCA AGAGAACAAA 
GCTACTGGGT TTCAGTTTGG TGCAAATAGT ATTCAGGAGC CTCCTAAGGA AACAGATTTA 
TCAAAAAATC TATTTTTTCA GTGTATGTCA CAAAATATCT CCCCAAGTAC CTATTTTACT 
TCCATATCAG AGAGTGCTAC TAGTGCTCCA GAAGAGCAAC CTAACAAAGA ACTTCCAAAG 
CAAAATAAGG AGGGACTGGC TTCTGGTTTC GGAAAACTTA AACCCCCAGT GACACTGGAA 
AGTAAAGCAA ATTCAAAGAT GTCCTTTTCT GAATCAATGC CAAAACTAAC ACCTGCCTTC 
CCTGGGAAAG TTGTAAGTGC ACGACCTGCA GAGACTCATG AAAATCTGTT TCTTCAGCCC 
CCAAAACTTT CAAGGGAAGA ACCTTCAAAT CCCTTCTTGG CTTTTTCAGA GAAAAAAGAC 
ATTTCACCAT TTGGCTTTTC TTCTCAAACA CCTCAAAGTT CCTTTTCTAG TGCTCTCCCT 
TCTGCTTGGA CAGAACCACG CAAGTTAGAT GACTCAGGGG CTTTGAAGAA ATCTTTGTTT 
CTTTCTGTGG AATCTCCAAA AAGATTGCCT GTTTCTCCTG GTTCATCAAT CAGTGAAACT 
GTACAAAGTT CTGCCTTTCT TGGAAATGGG CGTTCCAGCT CACCAGCAAA CAGCTTAAGT 
CAGCCTATTG AAATGCCTAC ATTGTCATCT AGCCCTACTG AGGATAAGCC TGCAATTGGA 
TCTGGACAGA TAGACAATCC GTTATTAAGA ACCTTTACAG TACTCTCAAG TAGGCTCCAA 
CCTGGTGTCC ACTGCTCCTC AGATTACCCT CAAGAAAACA AAGCAGCTTT TGAATCTGTT 
AACAGATTTA CCCTAGATGA ACGAAGTAGG ACATCAAAAC AAGATTCTGA CTCCAGCACC 
AACAGTGATT TAAGTGATCT AAGTGATTCT GAGGAGCAGC TGAATTCAAA GATTGGCTAT 
AGAGGAATAC CGGAACATCT GGTTGGAAGA TTAGGGCATA ATGGAGAACG TAACGTTGAT 
TTGTTAATGG CAAAAGGTAG AGGAAAACAG CCTTCAAAAG GCAGACCAAG AACAGCTCCG 
CTAAAGGTTG GTCAATCCGT TTTGAAAGAC ATGAGCAAAG TAAGAAAGTT AAAACAGTCT 
GGAGAGCCTT TTCTTCAAGA TGGGTCTTGT ATTAATGTTG CGCCACATTT GCACAAGTGC 
CGTGAATGCA GGCTAGAAAG ATACCGCAAG TTCAAAGAGC AGGATCAGGA CGATTCAACT 
GTAGCTTGTC GATTCTTTCA TTTTCGCAGG CTGGCTTTTA CCAGGAAAGG TATTCTTCGT 
GTTGAGGGCT TCTTAAGTCC CCAGCAAAGT GATGCTGAAG CAATGCGCCT CTGGGTTCCT 
ATTTCTGTTC CAGCGGACGG CATTGATTTG GAGACCTCAA AATATATTCT AGCTAATGTT 
GGAGATCAGT TTTGCCAGTT GGTCATGTCT GAAAAAGAAG CCATGATGAT GGTGGAACCC 
CAGCAAAAGG TAGCATGGAA GAGAGCTGTA CGTGGTGTCA GGGAGATGTG TGATGTATGT 
GAGACGACAC TCTTCAACAT TCACTGGGTT TGTCGCAAGT GTGGCTTTGG CGTATGTCTT 
GATTGTTATC GGCTCCGAAA AAATCGACCT CGCAGAGAAG ATGAAATTGG TGATGAAGAA 
GTCTTTGCGT GGCTAAAATG TGCGAAAGGA CAACCTCACG AACCAGAGAA TCTTATGCCT 
ACCCAGATTA TTCCAGGCAC TGCTCTTTAC AACATTGGGG ATATGGTCCA TGCAGCACGA 
GGGAAATGGG GCATTAAAGC CAATTGTCCA TGTATTAACA GACAGAATAA ATCTGTATTA 
AGGCCAGCAG TCACAAATGG AATGTCTCAG CTGCCTAATG CTAATAATGC TGCTGCTGAT 
ACAACTACTA CCGCAGATGA GGGGATTGAA AATAAATCAG ACCAGCCTTT AAAGAAAGAG 
GAAACAGAAA ATGAGGCATC GTCCAATAAT GATATCGATA TTGCTGCAAA TAGCAGCACG 
GATAGCAAAA ACAGCCGTCC TCAATGCCCG GAATCTACTC CTTCCTCTGC CTTACATTGG 
CTAGCAGACC TTGCAGCCCA GAAAGCAAAA GAAGAGACAA AAGACAATGG AACACCACAA 
TCAACACCCA GCAAAGAGTC AAGTCCACCT TCTGGACTGG ACCCATTAAA TGCAAATGTT 
AAGACTTCAT CTCCAAAACT TTTTAATAGC CTGTTATTGG GACCAGCTGC TTCAAATACT 
AAGACAGAAG GCTCCAGTCT TAGAGATCTG CTCCATTCTG GCCAAGGAAA ACTTTCTCAA 
GGAAATATGG AAGCAGGAAT TCCATTCCCA CCTGTCTTTT CCACAAGTTC AGCAAAGGGG 
AAAACAAGTT TACCAAATTT CCTTGATCAC ATTATTGCTT CTGTGGTCGA AAATAAAAAG 
ACTTCAGATG CCGCTAAAAG AGCCAGTAGC TCAACGGAAG CTCTGAGAGA GGGAAAGGAA 
ATGGTCATGG GATTAAATGT CTTTGATCCA CACACTTCAC ATTCTTGGCT CTGTGATGGA 
AGGCTGCTGT GCCTTCATGA TCCAAGCAAC AAGAATAATT GGAAGATATT CAGGGAGTGT 
TGGAAACAGG GTCAGCCTGT ACTTGTTTCT GGAGTACAGA AAAAGCTAAA GTCTGAACTT 
TGGAAACCTG AAGCTTTTAG CGTTGAGTTT GGAGACCAAG ATGTGGATTT GGTGAACTGT 
AGAAATTGTG CTATCATTTC TGATGTGAAA GTTCGTGATT TTTGGGATGG CTTTGCAGTC 
ATTGAAAAGA GGTTAAGAGC AGAAGATGGA AGTCCAATGG TCCTCAAGCT AAAAGACTGG 
CCACCTGGAG AAGACTTTCG GGATATGATG CCCACTCGAT TTCAAGACTT GATGGATAAT 
CTTCCACTAC CAGAATACAC AAAGAGAGAC GGCAGGCTCA ATTTGGCTTC AAGATTGCCT 
AGTTATTTTG TACGACCAGA TTTAGGGCCT AAAATGTACA ATGCTTATGG TTTAATTACT 
GCTGAAGACC GGCGCGTAGG TACTACTAAC CTACATTTGG ATGTGTCTGA TGCAGTTAAT 
GTTATGGTAT ATGTGGGGAT TCCAGTAGGG GAAAGCAGTC AAAATGAAGA GGTTTTAAGA 
ACGATTGAGG AGGGTGATGC TGATGAAGTA ACAAAAAAAC GTATTCATGA TGGAAAGGAG 
AAGCCTGGTG CTTTGTGGCA TATTTATGCT GCAAAGGACG CAGAGAAGAT TAGAGAGCTA 
CTCCGCAAGG TAGGTGATGA GCAAGGACAA GAAAACCCTC CAGACCATGA TCCAATCCAT 
GACCAAAGCT GGTATTTAGA CCAGGTACTG CGTAAGCGGC TGTATGATGA ATATGGTGTA 
CAAGGCTGGG CTATTGTACA GTTCTTGGGA GATGCTGTGT TCATTCCAGC TGGTGCACCC 
CATCAGGTGC ACAATTTGTA CAGTTGTATA AAGGTAGCAG AGGACTTTGT TTCTCCAGAA 
CATGTAAAGC ATTGCTTCCG CCTGACGCAG GAATTCAGGC ATTTGTCAAA CACTCACACG 
AACCATGAGG ATAAATTACA GGTGAAGAAT ATTATTTACC ATGCTGTAAA GGATGCTGTT 
GGAACACTAA AGGCTTTTGA GTGTAGATTG GTCAGAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936894.1
CDS	747..5828
Translation	MAVPVSPVGKRLRFLFDESASGREGATTEPSTPLGKRAWQGGTVRGLKGAVPEELAIYVEFDEKSYKSNSWVQVHGEDVLVILIEGALVWSPRKEPMFIQGSKLCPTHWPALTFTPLVDKLGLGTLIPIEFLLEEVLHFLPDASSLKLYQQMGADSHKLVLKEQPLLKDAVTSVINSRKTQDLILRGICSIQGQEVKVYQPDTEVPWSCGVVTHQDLITRMINVSMNEGNEIKSIDPKLIHVVLTDDTLIQPEEATKAGKASKSKKKKENTDARRRKLPSDAENDSAGKKQKGDKKDVDSNGNDGSEANRGVWKAGSTGEATPDSRSKQMLHFVPQINRNIRFATYTKENGRTLVVQDQPVNDGTSLAFPTFSGMTKGILSGESVGNSESVTSSGTTPTTVRISDTSLSALTGEEKKPNWSLGENLRKSLLTKSGFGPSLSQPLFGTDKNLSNGALQENKATGFQFGANSIQEPPKETDLSKNLFFQCMSQNISPSTYFTSISESATSAPEEQPNKELPKQNKEGLASGFGKLKPPVTLESKANSKMSFSESMPKLTPAFPGKVVSARPAETHENLFLQPPKLSREEPSNPFLAFSEKKDISPFGFSSQTPQSSFSSALPSAWTEPRKLDDSGALKKSLFLSVESPKRLPVSPGSSISETVQSSAFLGNGRSSSPANSLSQPIEMPTLSSSPTEDKPAIGSGQIDNPLLRTFTVLSSRLQPGVHCSSDYPQENKAAFESVNRFTLDERSRTSKQDSDSSTNSDLSDLSDSEEQLNSKIGYRGIPEHLVGRLGHNGERNVDLLMAKGRGKQPSKGRPRTAPLKVGQSVLKDMSKVRKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQDQDDSTVACRFFHFRRLAFTRKGILRVEGFLSPQQSDAEAMRLWVPISVPADGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPQQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPRREDEIGDEEVFAWLKCAKGQPHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCINRQNKSVLRPAVTNGMSQLPNANNAAADTTTTADEGIENKSDQPLKKEETENEASSNNDIDIAANSSTDSKNSRPQCPESTPSSALHWLADLAAQKAKEETKDNGTPQSTPSKESSPPSGLDPLNANVKTSSPKLFNSLLLGPAASNTKTEGSSLRDLLHSGQGKLSQGNMEAGIPFPPVFSTSSAKGKTSLPNFLDHIIASVVENKKTSDAAKRASSSTEALREGKEMVMGLNVFDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVQKKLKSELWKPEAFSVEFGDQDVDLVNCRNCAIISDVKVRDFWDGFAVIEKRLRAEDGSPMVLKLKDWPPGEDFRDMMPTRFQDLMDNLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPVGESSQNEEVLRTIEEGDADEVTKKRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGDEQGQENPPDHDPIHDQSWYLDQVLRKRLYDEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAFECRLVRT

Target ORF information:

RefSeq Version	XM_002936848.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936848.5

ORF Insert Sequence:

ATGGCGGTCC CGGTTTCCCC GGTGGGGAAG AGGCTTCGGT TTCTATTTGA CGAATCGGCC 
TCTGGGCGGG AGGGGGCAAC CACTGAACCG TCCACTCCGC TGGGGAAGCG GGCCTGGCAG 
GGCGGGACGG TGCGGGGCCT GAAAGGAGCG GTGCCCGAAG AGCTAGCGAT TTATGTGGAG 
TTTGATGAGA AAAGTTACAA GTCAAATTCT TGGGTGCAGG TTCATGGGGA GGATGTGTTG 
GTGATCCTGA TAGAAGGAGC TTTGGTTTGG TCTCCAAGAA AAGAGCCTAT GTTTATCCAA 
GGAAGCAAAT TATGTCCAAC TCATTGGCCA GCTTTAACCT TTACTCCACT GGTGGACAAG 
TTGGGCTTAG GGACTTTAAT TCCAATAGAA TTTCTTTTGG AAGAGGTTCT GCATTTCCTA 
CCAGATGCCA GCTCTCTGAA ACTTTATCAG CAAATGGGAG CAGACAGTCA TAAACTGGTT 
TTGAAAGAGC AGCCTTTGTT AAAGGATGCA GTGACTTCTG TGATCAATTC AAGGAAAACA 
CAAGATCTCA TTTTAAGAGG TATTTGCAGT ATTCAAGGCC AGGAAGTTAA GGTTTATCAA 
CCAGATACAG AAGTGCCATG GTCTTGTGGA GTTGTGACCC ATCAGGATCT TATTACACGC 
ATGATAAATG TATCAATGAA TGAGGGAAAT GAGATCAAGT CTATTGATCC AAAATTAATT 
CATGTGGTGC TTACTGACGA CACTTTAATA CAACCTGAGG AGGCTACAAA AGCAGGTAAA 
GCATCTAAAT CAAAGAAAAA AAAGGAGAAT ACAGATGCTC GCAGAAGAAA ATTACCTTCA 
GATGCTGAAA ATGATTCTGC TGGAAAAAAG CAGAAAGGCG ATAAGAAAGA TGTGGACAGT 
AATGGAAATG ATGGCAGTGA AGCTAATAGA GGAGTTTGGA AAGCAGGCAG CACTGGAGAA 
GCTACACCAG ATTCAAGATC TAAGCAAATG TTACACTTTG TTCCTCAAAT TAATCGCAAT 
ATCCGATTTG CAACCTACAC CAAAGAGAAT GGACGCACAT TAGTGGTTCA AGATCAGCCT 
GTAAATGATG GAACCTCATT AGCTTTTCCA ACTTTTTCTG GAATGACTAA AGGTATATTG 
TCTGGTGAAA GTGTTGGGAA CTCTGAGAGT GTAACTTCGA GTGGTACTAC TCCTACCACA 
GTACGAATCT CTGATACTAG TCTCTCTGCT TTGACTGGAG AAGAAAAGAA GCCAAACTGG 
TCCCTTGGAG AGAACCTCAG AAAATCACTG CTGACCAAGA GTGGATTTGG CCCATCACTC 
TCTCAGCCAT TATTTGGGAC TGATAAAAAC CTATCTAATG GTGCACTTCA AGAGAACAAA 
GCTACTGGGT TTCAGTTTGG TGCAAATAGT ATTCAGGAGC CTCCTAAGGA AACAGATTTA 
TCAAAAAATC TATTTTTTCA GTGTATGTCA CAAAATATCT CCCCAAGTAC CTATTTTACT 
TCCATATCAG AGAGTGCTAC TAGTGCTCCA GAAGAGCAAC CTAACAAAGA ACTTCCAAAG 
CAAAATAAGG AGGGACTGGC TTCTGGTTTC GGAAAACTTA AACCCCCAGT GACACTGGAA 
AGTAAAGCAA ATTCAAAGAT GTCCTTTTCT GAATCAATGC CAAAACTAAC ACCTGCCTTC 
CCTGGGAAAG TTGTAAGTGC ACGACCTGCA GAGACTCATG AAAATCTGTT TCTTCAGCCC 
CCAAAACTTT CAAGGGAAGA ACCTTCAAAT CCCTTCTTGG CTTTTTCAGA GAAAAAAGAC 
ATTTCACCAT TTGGCTTTTC TTCTCAAACA CCTCAAAGTT CCTTTTCTAG TGCTCTCCCT 
TCTGCTTGGA CAGAACCACG CAAGTTAGAT GACTCAGGGG CTTTGAAGAA ATCTTTGTTT 
CTTTCTGTGG AATCTCCAAA AAGATTGCCT GTTTCTCCTG GTTCATCAAT CAGTGAAACT 
GTACAAAGTT CTGCCTTTCT TGGAAATGGG CGTTCCAGCT CACCAGCAAA CAGCTTAAGT 
CAGCCTATTG AAATGCCTAC ATTGTCATCT AGCCCTACTG AGGATAAGCC TGCAATTGGA 
TCTGGACAGA TAGACAATCC GTTATTAAGA ACCTTTACAG TACTCTCAAG TAGGCTCCAA 
CCTGGTGTCC ACTGCTCCTC AGATTACCCT CAAGAAAACA AAGCAGCTTT TGAATCTGTT 
AACAGATTTA CCCTAGATGA ACGAAGTAGG ACATCAAAAC AAGATTCTGA CTCCAGCACC 
AACAGTGATT TAAGTGATCT AAGTGATTCT GAGGAGCAGC TGAATTCAAA GATTGGCTAT 
AGAGGAATAC CGGAACATCT GGTTGGAAGA TTAGGGCATA ATGGAGAACG TAACGTTGAT 
TTGTTAATGG CAAAAGGTAG AGGAAAACAG CCTTCAAAAG GCAGACCAAG AACAGCTCCG 
CTAAAGGTTG GTCAATCCGT TTTGAAAGAC ATGAGCAAAG TAAGAAAGTT AAAACAGTCT 
GGAGAGCCTT TTCTTCAAGA TGGGTCTTGT ATTAATGTTG CGCCACATTT GCACAAGTGC 
CGTGAATGCA GGCTAGAAAG ATACCGCAAG TTCAAAGAGC AGGATCAGGA CGATTCAACT 
GTAGCTTGTC GATTCTTTCA TTTTCGCAGG CTGGCTTTTA CCAGGAAAGG TATTCTTCGT 
GTTGAGGGCT TCTTAAGTCC CCAGCAAAGT GATGCTGAAG CAATGCGCCT CTGGGTTCCT 
ATTTCTGTTC CAGCGGACGG CATTGATTTG GAGACCTCAA AATATATTCT AGCTAATGTT 
GGAGATCAGT TTTGCCAGTT GGTCATGTCT GAAAAAGAAG CCATGATGAT GGTGGAACCC 
CAGCAAAAGG TAGCATGGAA GAGAGCTGTA CGTGGTGTCA GGGAGATGTG TGATGTATGT 
GAGACGACAC TCTTCAACAT TCACTGGGTT TGTCGCAAGT GTGGCTTTGG CGTATGTCTT 
GATTGTTATC GGCTCCGAAA AAATCGACCT CGCAGAGAAG ATGAAATTGG TGATGAAGAA 
GTCTTTGCGT GGCTAAAATG TGCGAAAGGA CAACCTCACG AACCAGAGAA TCTTATGCCT 
ACCCAGATTA TTCCAGGCAC TGCTCTTTAC AACATTGGGG ATATGGTCCA TGCAGCACGA 
GGGAAATGGG GCATTAAAGC CAATTGTCCA TGTATTAACA GACAGAATAA ATCTGTATTA 
AGGCCAGCAG TCACAAATGG AATGTCTCAG CTGCCTAATG CTAATAATGC TGCTGCTGAT 
ACAACTACTA CCGCAGATGA GGGGATTGAA AATAAATCAG ACCAGCCTTT AAAGAAAGAG 
GAAACAGAAA ATGAGGCATC GTCCAATAAT GATATCGATA TTGCTGCAAA TAGCAGCACG 
GATAGCAAAA ACAGCCGTCC TCAATGCCCG GAATCTACTC CTTCCTCTGC CTTACATTGG 
CTAGCAGACC TTGCAGCCCA GAAAGCAAAA GAAGAGACAA AAGACAATGG AACACCACAA 
TCAACACCCA GCAAAGAGTC AAGTCCACCT TCTGGACTGG ACCCATTAAA TGCAAATGTT 
AAGACTTCAT CTCCAAAACT TTTTAATAGC CTGTTATTGG GACCAGCTGC TTCAAATACT 
AAGACAGAAG GCTCCAGTCT TAGAGATCTG CTCCATTCTG GCCAAGGAAA ACTTTCTCAA 
GGAAATATGG AAGCAGGAAT TCCATTCCCA CCTGTCTTTT CCACAAGTTC AGCAAAGGGG 
AAAACAAGTT TACCAAATTT CCTTGATCAC ATTATTGCTT CTGTGGTCGA AAATAAAAAG 
ACTTCAGATG CCGCTAAAAG AGCCAGTAGC TCAACGGAAG CTCTGAGAGA GGGAAAGGAA 
ATGGTCATGG GATTAAATGT CTTTGATCCA CACACTTCAC ATTCTTGGCT CTGTGATGGA 
AGGCTGCTGT GCCTTCATGA TCCAAGCAAC AAGAATAATT GGAAGATATT CAGGGAGTGT 
TGGAAACAGG GTCAGCCTGT ACTTGTTTCT GGAGTACAGA AAAAGCTAAA GTCTGAACTT 
TGGAAACCTG AAGCTTTTAG CGTTGAGTTT GGAGACCAAG ATGTGGATTT GGTGAACTGT 
AGAAATTGTG CTATCATTTC TGATGTGAAA GTTCGTGATT TTTGGGATGG CTTTGCAGTC 
ATTGAAAAGA GGTTAAGAGC AGAAGATGGA AGTCCAATGG TCCTCAAGCT AAAAGACTGG 
CCACCTGGAG AAGACTTTCG GGATATGATG CCCACTCGAT TTCAAGACTT GATGGATAAT 
CTTCCACTAC CAGAATACAC AAAGAGAGAC GGCAGGCTCA ATTTGGCTTC AAGATTGCCT 
AGTTATTTTG TACGACCAGA TTTAGGGCCT AAAATGTACA ATGCTTATGG TTTAATTACT 
GCTGAAGACC GGCGCGTAGG TACTACTAAC CTACATTTGG ATGTGTCTGA TGCAGTTAAT 
GTTATGGTAT ATGTGGGGAT TCCAGTAGGG GAAAGCAGTC AAAATGAAGA GGTTTTAAGA 
ACGATTGAGG AGGGTGATGC TGATGAAGTA ACAAAAAAAC GTATTCATGA TGGAAAGGAG 
AAGCCTGGTG CTTTGTGGCA TATTTATGCT GCAAAGGACG CAGAGAAGAT TAGAGAGCTA 
CTCCGCAAGG TAGGTGATGA GCAAGGACAA GAAAACCCTC CAGACCATGA TCCAATCCAT 
GACCAAAGCT GGTATTTAGA CCAGGTACTG CGTAAGCGGC TGTATGATGA ATATGGTGTA 
CAAGGCTGGG CTATTGTACA GTTCTTGGGA GATGCTGTGT TCATTCCAGC TGGTGCACCC 
CATCAGGTGC ACAATTTGTA CAGTTGTATA AAGGTAGCAG AGGACTTTGT TTCTCCAGAA 
CATGTAAAGC ATTGCTTCCG CCTGACGCAG GAATTCAGGC ATTTGTCAAA CACTCACACG 
AACCATGAGG ATAAATTACA GGTGAAGAAT ATTATTTACC ATGCTGTAAA GGATGCTGTT 
GGAACACTAA AGGCTTTTGA GTGTAGATTG GTCAGAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_004912889.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912946.1 Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.

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CloneID	OXa07679
Clone ID Related Accession (Same CDS sequence)	XM_004912889.3 , XM_004912889.4
Accession Version	XM_004912889.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912889.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTCC CGGTTTCCCC GGTGGGGAAG AGGCTTCGGT TTCTATTTGA CGAATCGGCC 
TCTGGGCGGG AGGGGGCAAC CACTGAACCG TCCACTCCGC TGGGGAAGCG GGCCTGGCAG 
GGCGGGACGG TGCGGGGCCT GAAAGGAGCG GTGCCCGAAG AGCTAGCGAT TTATGTGGAG 
TTTGATGAGA AAAGTTACAA GTCAAATTCT TGGGTGCAGG TTCATGGGGA GGATGTGTTG 
GTGATCCTGA TAGAAGGAGC TTTGGTTTGG TCTCCAAGAA AAGAGCCTAT GTTTATCCAA 
GGAAGCAAAT TATGTCCAAC TCATTGGCCA GCTTTAACCT TTACTCCACT GGTGGACAAG 
TTGGGCTTAG GGACTTTAAT TCCAATAGAA TTTCTTTTGG AAGAGGTTCT GCATTTCCTA 
CCAGATGCCA GCTCTCTGAA ACTTTATCAG CAAATGGGAG CAGACAGTCA TAAACTGGTT 
TTGAAAGAGC AGCCTTTGTT AAAGGATGCA GTGACTTCTG TGATCAATTC AAGGAAAACA 
CAAGATCTCA TTTTAAGAGG TATTTGCAGT ATTCAAGGCC AGGAAGTTAA GGTTTATCAA 
CCAGATACAG AAGTGCCATG GTCTTGTGGA GTTGTGACCC ATCAGGATCT TATTACACGC 
ATGATAAATG TATCAATGAA TGAGGGAAAT GAGATCAAGT CTATTGATCC AAAATTAATT 
CATGTGGTGC TTACTGACGA CACTTTAATA CAACCTGAGG AGGCTACAAA AGCAGGTAAA 
GCATCTAAAT CAAAGAAAAA AAAGGAGAAT ACAGATGCTC GCAGAAGAAA ATTACCTTCA 
GATGCTGAAA ATGATTCTGC TGGAAAAAAG CAGAAAGGCG ATAAGAAAGA TGTGGACAGT 
AATGGAAATG ATGGCAGTGA AGCTAATAGA GGAGTTTGGA AAGCAGGCAG CACTGGAGAA 
GCTACACCAG ATTCAAGATC TAAGCAAATG TTACACTTTG TTCCTCAAAT TAATCGCAAT 
ATCCGATTTG CAACCTACAC CAAAGAGAAT GGACGCACAT TAGTGGTTCA AGATCAGCCT 
GTAAATGATG GAACCTCATT AGCTTTTCCA ACTTTTTCTG GAATGACTAA AGGTATATTG 
TCTGGTGAAA GTGTTGGGAA CTCTGAGAGT GTAACTTCGA GTGGTACTAC TCCTACCACA 
GTACGAATCT CTGATACTAG TCTCTCTGCT TTGACTGGAG AAGAAAAGAA GCCAAACTGG 
TCCCTTGGAG AGAACCTCAG AAAATCACTG CTGACCAAGA GTGGATTTGG CCCATCACTC 
TCTCAGCCAT TATTTGGGAC TGATAAAAAC CTATCTAATG GTGCACTTCA AGAGAACAAA 
GCTACTGGGT TTCAGTTTGG TGCAAATAGT ATTCAGGAGC CTCCTAAGGA AACAGATTTA 
TCAAAAAATC TATTTTTTCA GTGTATGTCA CAAAATATCT CCCCAAGTAC CTATTTTACT 
TCCATATCAG AGAGTGCTAC TAGTGCTCCA GAAGAGCAAC CTAACAAAGA ACTTCCAAAG 
CAAAATAAGG AGGGACTGGC TTCTGGTTTC GGAAAACTTA AACCCCCAGT GACACTGGAA 
AGTAAAGCAA ATTCAAAGAT GTCCTTTTCT GAATCAATGC CAAAACTAAC ACCTGCCTTC 
CCTGGGAAAG TTGTAAGTGC ACGACCTGCA GAGACTCATG AAAATCTGTT TCTTCAGCCC 
CCAAAACTTT CAAGGGAAGA ACCTTCAAAT CCCTTCTTGG CTTTTTCAGA GAAAAAAGAC 
ATTTCACCAT TTGGCTTTTC TTCTCAAACA CCTCAAAGTT CCTTTTCTAG TGCTCTCCCT 
TCTGCTTGGA CAGAACCACG CAAGTTAGAT GACTCAGGGG CTTTGAAGAA ATCTTTGTTT 
CTTTCTGTGG AATCTCCAAA AAGATTGCCT GTTTCTCCTG GTTCATCAAT CAGTGAAACT 
GTACAAAGTT CTGCCTTTCT TGGAAATGGG CGTTCCAGCT CACCAGCAAA CAGCTTAAGT 
CAGCCTATTG AAATGCCTAC ATTGTCATCT AGCCCTACTG AGGATAAGCC TGCAATTGGA 
TCTGGACAGA TAGACAATCC GTTATTAAGA ACCTTTACAG TACTCTCAAG TAGGCTCCAA 
CCTGGTGTCC ACTGCTCCTC AGATTACCCT CAAGAAAACA AAGCAGCTTT TGAATCTGTT 
AACAGATTTA CCCTAGATGA ACGAAGTAGG ACATCAAAAC AAGATTCTGA CTCCAGCACC 
AACAGTGATT TAAGTGATCT AAGTGATTCT GAGGAGCAGC TGAATTCAAA GATTGGCTAT 
AGAGGAATAC CGGAACATCT GGTTGGAAGA TTAGGGCATA ATGGAGAACG TAACGTTGAT 
TTGTTAATGG CAAAAGGTAG AGGAAAACAG CCTTCAAAAG GCAGACCAAG AACAGCTCCG 
CTAAAGGTTG GTCAATCCGT TTTGAAAGAC ATGAGCAAAG TAAGAAAGTT AAAACAGTCT 
GGAGAGCCTT TTCTTCAAGA TGGGTCTTGT ATTAATGTTG CGCCACATTT GCACAAGTGC 
CGTGAATGCA GGCTAGAAAG ATACCGCAAG TTCAAAGAGC AGGATCAGGA CGATTCAACT 
GTAGCTTGTC GATTCTTTCA TTTTCGCAGG CTGGCTTTTA CCAGGAAAGG TATTCTTCGT 
GTTGAGGGCT TCTTAAGTCC CCAGCAAAGT GATGCTGAAG CAATGCGCCT CTGGGTTCCT 
ATTTCTGTTC CAGCGGACGG CATTGATTTG GAGACCTCAA AATATATTCT AGCTAATGTT 
GGAGATCAGT TTTGCCAGTT GGTCATGTCT GAAAAAGAAG CCATGATGAT GGTGGAACCC 
CAGCAAAAGG TAGCATGGAA GAGAGCTGTA CGTGGTGTCA GGGAGATGTG TGATGTATGT 
GAGACGACAC TCTTCAACAT TCACTGGGTT TGTCGCAAGT GTGGCTTTGG CGTATGTCTT 
GATTGTTATC GGCTCCGAAA AAATCGACCT CGCAGAGATG AAATTGGTGA TGAAGAAGTC 
TTTGCGTGGC TAAAATGTGC GAAAGGACAA CCTCACGAAC CAGAGAATCT TATGCCTACC 
CAGATTATTC CAGGCACTGC TCTTTACAAC ATTGGGGATA TGGTCCATGC AGCACGAGGG 
AAATGGGGCA TTAAAGCCAA TTGTCCATGT ATTAACAGAC AGAATAAATC TGTATTAAGG 
CCAGCAGTCA CAAATGGAAT GTCTCAGCTG CCTAATGCTA ATAATGCTGC TGCTGATACA 
ACTACTACCG CAGATGAGGG GATTGAAAAT AAATCAGACC AGCCTTTAAA GAAAGAGGAA 
ACAGAAAATG AGGCATCGTC CAATAATGAT ATCGATATTG CTGCAAATAG CAGCACGGAT 
AGCAAAAACA GCCGTCCTCA ATGCCCGGAA TCTACTCCTT CCTCTGCCTT ACATTGGCTA 
GCAGACCTTG CAGCCCAGAA AGCAAAAGAA GAGACAAAAG ACAATGGAAC ACCACAATCA 
ACACCCAGCA AAGAGTCAAG TCCACCTTCT GGACTGGACC CATTAAATGC AAATGTTAAG 
ACTTCATCTC CAAAACTTTT TAATAGCCTG TTATTGGGAC CAGCTGCTTC AAATACTAAG 
ACAGAAGGCT CCAGTCTTAG AGATCTGCTC CATTCTGGCC AAGGAAAACT TTCTCAAGGA 
AATATGGAAG CAGGAATTCC ATTCCCACCT GTCTTTTCCA CAAGTTCAGC AAAGGGGAAA 
ACAAGTTTAC CAAATTTCCT TGATCACATT ATTGCTTCTG TGGTCGAAAA TAAAAAGACT 
TCAGATGCCG CTAAAAGAGC CAGTAGCTCA ACGGAAGCTC TGAGAGAGGG AAAGGAAATG 
GTCATGGGAT TAAATGTCTT TGATCCACAC ACTTCACATT CTTGGCTCTG TGATGGAAGG 
CTGCTGTGCC TTCATGATCC AAGCAACAAG AATAATTGGA AGATATTCAG GGAGTGTTGG 
AAACAGGGTC AGCCTGTACT TGTTTCTGGA GTACAGAAAA AGCTAAAGTC TGAACTTTGG 
AAACCTGAAG CTTTTAGCGT TGAGTTTGGA GACCAAGATG TGGATTTGGT GAACTGTAGA 
AATTGTGCTA TCATTTCTGA TGTGAAAGTT CGTGATTTTT GGGATGGCTT TGCAGTCATT 
GAAAAGAGGT TAAGAGCAGA AGATGGAAGT CCAATGGTCC TCAAGCTAAA AGACTGGCCA 
CCTGGAGAAG ACTTTCGGGA TATGATGCCC ACTCGATTTC AAGACTTGAT GGATAATCTT 
CCACTACCAG AATACACAAA GAGAGACGGC AGGCTCAATT TGGCTTCAAG ATTGCCTAGT 
TATTTTGTAC GACCAGATTT AGGGCCTAAA ATGTACAATG CTTATGGTTT AATTACTGCT 
GAAGACCGGC GCGTAGGTAC TACTAACCTA CATTTGGATG TGTCTGATGC AGTTAATGTT 
ATGGTATATG TGGGGATTCC AGTAGGGGAA AGCAGTCAAA ATGAAGAGGT TTTAAGAACG 
ATTGAGGAGG GTGATGCTGA TGAAGTAACA AAAAAACGTA TTCATGATGG AAAGGAGAAG 
CCTGGTGCTT TGTGGCATAT TTATGCTGCA AAGGACGCAG AGAAGATTAG AGAGCTACTC 
CGCAAGGTAG GTGATGAGCA AGGACAAGAA AACCCTCCAG ACCATGATCC AATCCATGAC 
CAAAGCTGGT ATTTAGACCA GGTACTGCGT AAGCGGCTGT ATGATGAATA TGGTGTACAA 
GGCTGGGCTA TTGTACAGTT CTTGGGAGAT GCTGTGTTCA TTCCAGCTGG TGCACCCCAT 
CAGGTGCACA ATTTGTACAG TTGTATAAAG GTAGCAGAGG ACTTTGTTTC TCCAGAACAT 
GTAAAGCATT GCTTCCGCCT GACGCAGGAA TTCAGGCATT TGTCAAACAC TCACACGAAC 
CATGAGGATA AATTACAGGT GAAGAATATT ATTTACCATG CTGTAAAGGA TGCTGTTGGA 
ACACTAAAGG CTTTTGAGTG TAGATTGGTC AGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912946.1
CDS	747..5825
Translation	MAVPVSPVGKRLRFLFDESASGREGATTEPSTPLGKRAWQGGTVRGLKGAVPEELAIYVEFDEKSYKSNSWVQVHGEDVLVILIEGALVWSPRKEPMFIQGSKLCPTHWPALTFTPLVDKLGLGTLIPIEFLLEEVLHFLPDASSLKLYQQMGADSHKLVLKEQPLLKDAVTSVINSRKTQDLILRGICSIQGQEVKVYQPDTEVPWSCGVVTHQDLITRMINVSMNEGNEIKSIDPKLIHVVLTDDTLIQPEEATKAGKASKSKKKKENTDARRRKLPSDAENDSAGKKQKGDKKDVDSNGNDGSEANRGVWKAGSTGEATPDSRSKQMLHFVPQINRNIRFATYTKENGRTLVVQDQPVNDGTSLAFPTFSGMTKGILSGESVGNSESVTSSGTTPTTVRISDTSLSALTGEEKKPNWSLGENLRKSLLTKSGFGPSLSQPLFGTDKNLSNGALQENKATGFQFGANSIQEPPKETDLSKNLFFQCMSQNISPSTYFTSISESATSAPEEQPNKELPKQNKEGLASGFGKLKPPVTLESKANSKMSFSESMPKLTPAFPGKVVSARPAETHENLFLQPPKLSREEPSNPFLAFSEKKDISPFGFSSQTPQSSFSSALPSAWTEPRKLDDSGALKKSLFLSVESPKRLPVSPGSSISETVQSSAFLGNGRSSSPANSLSQPIEMPTLSSSPTEDKPAIGSGQIDNPLLRTFTVLSSRLQPGVHCSSDYPQENKAAFESVNRFTLDERSRTSKQDSDSSTNSDLSDLSDSEEQLNSKIGYRGIPEHLVGRLGHNGERNVDLLMAKGRGKQPSKGRPRTAPLKVGQSVLKDMSKVRKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQDQDDSTVACRFFHFRRLAFTRKGILRVEGFLSPQQSDAEAMRLWVPISVPADGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPQQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPRRDEIGDEEVFAWLKCAKGQPHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCINRQNKSVLRPAVTNGMSQLPNANNAAADTTTTADEGIENKSDQPLKKEETENEASSNNDIDIAANSSTDSKNSRPQCPESTPSSALHWLADLAAQKAKEETKDNGTPQSTPSKESSPPSGLDPLNANVKTSSPKLFNSLLLGPAASNTKTEGSSLRDLLHSGQGKLSQGNMEAGIPFPPVFSTSSAKGKTSLPNFLDHIIASVVENKKTSDAAKRASSSTEALREGKEMVMGLNVFDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVQKKLKSELWKPEAFSVEFGDQDVDLVNCRNCAIISDVKVRDFWDGFAVIEKRLRAEDGSPMVLKLKDWPPGEDFRDMMPTRFQDLMDNLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPVGESSQNEEVLRTIEEGDADEVTKKRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGDEQGQENPPDHDPIHDQSWYLDQVLRKRLYDEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAFECRLVRT

Target ORF information:

RefSeq Version	XM_004912889.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912889.4

ORF Insert Sequence:

ATGGCGGTCC CGGTTTCCCC GGTGGGGAAG AGGCTTCGGT TTCTATTTGA CGAATCGGCC 
TCTGGGCGGG AGGGGGCAAC CACTGAACCG TCCACTCCGC TGGGGAAGCG GGCCTGGCAG 
GGCGGGACGG TGCGGGGCCT GAAAGGAGCG GTGCCCGAAG AGCTAGCGAT TTATGTGGAG 
TTTGATGAGA AAAGTTACAA GTCAAATTCT TGGGTGCAGG TTCATGGGGA GGATGTGTTG 
GTGATCCTGA TAGAAGGAGC TTTGGTTTGG TCTCCAAGAA AAGAGCCTAT GTTTATCCAA 
GGAAGCAAAT TATGTCCAAC TCATTGGCCA GCTTTAACCT TTACTCCACT GGTGGACAAG 
TTGGGCTTAG GGACTTTAAT TCCAATAGAA TTTCTTTTGG AAGAGGTTCT GCATTTCCTA 
CCAGATGCCA GCTCTCTGAA ACTTTATCAG CAAATGGGAG CAGACAGTCA TAAACTGGTT 
TTGAAAGAGC AGCCTTTGTT AAAGGATGCA GTGACTTCTG TGATCAATTC AAGGAAAACA 
CAAGATCTCA TTTTAAGAGG TATTTGCAGT ATTCAAGGCC AGGAAGTTAA GGTTTATCAA 
CCAGATACAG AAGTGCCATG GTCTTGTGGA GTTGTGACCC ATCAGGATCT TATTACACGC 
ATGATAAATG TATCAATGAA TGAGGGAAAT GAGATCAAGT CTATTGATCC AAAATTAATT 
CATGTGGTGC TTACTGACGA CACTTTAATA CAACCTGAGG AGGCTACAAA AGCAGGTAAA 
GCATCTAAAT CAAAGAAAAA AAAGGAGAAT ACAGATGCTC GCAGAAGAAA ATTACCTTCA 
GATGCTGAAA ATGATTCTGC TGGAAAAAAG CAGAAAGGCG ATAAGAAAGA TGTGGACAGT 
AATGGAAATG ATGGCAGTGA AGCTAATAGA GGAGTTTGGA AAGCAGGCAG CACTGGAGAA 
GCTACACCAG ATTCAAGATC TAAGCAAATG TTACACTTTG TTCCTCAAAT TAATCGCAAT 
ATCCGATTTG CAACCTACAC CAAAGAGAAT GGACGCACAT TAGTGGTTCA AGATCAGCCT 
GTAAATGATG GAACCTCATT AGCTTTTCCA ACTTTTTCTG GAATGACTAA AGGTATATTG 
TCTGGTGAAA GTGTTGGGAA CTCTGAGAGT GTAACTTCGA GTGGTACTAC TCCTACCACA 
GTACGAATCT CTGATACTAG TCTCTCTGCT TTGACTGGAG AAGAAAAGAA GCCAAACTGG 
TCCCTTGGAG AGAACCTCAG AAAATCACTG CTGACCAAGA GTGGATTTGG CCCATCACTC 
TCTCAGCCAT TATTTGGGAC TGATAAAAAC CTATCTAATG GTGCACTTCA AGAGAACAAA 
GCTACTGGGT TTCAGTTTGG TGCAAATAGT ATTCAGGAGC CTCCTAAGGA AACAGATTTA 
TCAAAAAATC TATTTTTTCA GTGTATGTCA CAAAATATCT CCCCAAGTAC CTATTTTACT 
TCCATATCAG AGAGTGCTAC TAGTGCTCCA GAAGAGCAAC CTAACAAAGA ACTTCCAAAG 
CAAAATAAGG AGGGACTGGC TTCTGGTTTC GGAAAACTTA AACCCCCAGT GACACTGGAA 
AGTAAAGCAA ATTCAAAGAT GTCCTTTTCT GAATCAATGC CAAAACTAAC ACCTGCCTTC 
CCTGGGAAAG TTGTAAGTGC ACGACCTGCA GAGACTCATG AAAATCTGTT TCTTCAGCCC 
CCAAAACTTT CAAGGGAAGA ACCTTCAAAT CCCTTCTTGG CTTTTTCAGA GAAAAAAGAC 
ATTTCACCAT TTGGCTTTTC TTCTCAAACA CCTCAAAGTT CCTTTTCTAG TGCTCTCCCT 
TCTGCTTGGA CAGAACCACG CAAGTTAGAT GACTCAGGGG CTTTGAAGAA ATCTTTGTTT 
CTTTCTGTGG AATCTCCAAA AAGATTGCCT GTTTCTCCTG GTTCATCAAT CAGTGAAACT 
GTACAAAGTT CTGCCTTTCT TGGAAATGGG CGTTCCAGCT CACCAGCAAA CAGCTTAAGT 
CAGCCTATTG AAATGCCTAC ATTGTCATCT AGCCCTACTG AGGATAAGCC TGCAATTGGA 
TCTGGACAGA TAGACAATCC GTTATTAAGA ACCTTTACAG TACTCTCAAG TAGGCTCCAA 
CCTGGTGTCC ACTGCTCCTC AGATTACCCT CAAGAAAACA AAGCAGCTTT TGAATCTGTT 
AACAGATTTA CCCTAGATGA ACGAAGTAGG ACATCAAAAC AAGATTCTGA CTCCAGCACC 
AACAGTGATT TAAGTGATCT AAGTGATTCT GAGGAGCAGC TGAATTCAAA GATTGGCTAT 
AGAGGAATAC CGGAACATCT GGTTGGAAGA TTAGGGCATA ATGGAGAACG TAACGTTGAT 
TTGTTAATGG CAAAAGGTAG AGGAAAACAG CCTTCAAAAG GCAGACCAAG AACAGCTCCG 
CTAAAGGTTG GTCAATCCGT TTTGAAAGAC ATGAGCAAAG TAAGAAAGTT AAAACAGTCT 
GGAGAGCCTT TTCTTCAAGA TGGGTCTTGT ATTAATGTTG CGCCACATTT GCACAAGTGC 
CGTGAATGCA GGCTAGAAAG ATACCGCAAG TTCAAAGAGC AGGATCAGGA CGATTCAACT 
GTAGCTTGTC GATTCTTTCA TTTTCGCAGG CTGGCTTTTA CCAGGAAAGG TATTCTTCGT 
GTTGAGGGCT TCTTAAGTCC CCAGCAAAGT GATGCTGAAG CAATGCGCCT CTGGGTTCCT 
ATTTCTGTTC CAGCGGACGG CATTGATTTG GAGACCTCAA AATATATTCT AGCTAATGTT 
GGAGATCAGT TTTGCCAGTT GGTCATGTCT GAAAAAGAAG CCATGATGAT GGTGGAACCC 
CAGCAAAAGG TAGCATGGAA GAGAGCTGTA CGTGGTGTCA GGGAGATGTG TGATGTATGT 
GAGACGACAC TCTTCAACAT TCACTGGGTT TGTCGCAAGT GTGGCTTTGG CGTATGTCTT 
GATTGTTATC GGCTCCGAAA AAATCGACCT CGCAGAGATG AAATTGGTGA TGAAGAAGTC 
TTTGCGTGGC TAAAATGTGC GAAAGGACAA CCTCACGAAC CAGAGAATCT TATGCCTACC 
CAGATTATTC CAGGCACTGC TCTTTACAAC ATTGGGGATA TGGTCCATGC AGCACGAGGG 
AAATGGGGCA TTAAAGCCAA TTGTCCATGT ATTAACAGAC AGAATAAATC TGTATTAAGG 
CCAGCAGTCA CAAATGGAAT GTCTCAGCTG CCTAATGCTA ATAATGCTGC TGCTGATACA 
ACTACTACCG CAGATGAGGG GATTGAAAAT AAATCAGACC AGCCTTTAAA GAAAGAGGAA 
ACAGAAAATG AGGCATCGTC CAATAATGAT ATCGATATTG CTGCAAATAG CAGCACGGAT 
AGCAAAAACA GCCGTCCTCA ATGCCCGGAA TCTACTCCTT CCTCTGCCTT ACATTGGCTA 
GCAGACCTTG CAGCCCAGAA AGCAAAAGAA GAGACAAAAG ACAATGGAAC ACCACAATCA 
ACACCCAGCA AAGAGTCAAG TCCACCTTCT GGACTGGACC CATTAAATGC AAATGTTAAG 
ACTTCATCTC CAAAACTTTT TAATAGCCTG TTATTGGGAC CAGCTGCTTC AAATACTAAG 
ACAGAAGGCT CCAGTCTTAG AGATCTGCTC CATTCTGGCC AAGGAAAACT TTCTCAAGGA 
AATATGGAAG CAGGAATTCC ATTCCCACCT GTCTTTTCCA CAAGTTCAGC AAAGGGGAAA 
ACAAGTTTAC CAAATTTCCT TGATCACATT ATTGCTTCTG TGGTCGAAAA TAAAAAGACT 
TCAGATGCCG CTAAAAGAGC CAGTAGCTCA ACGGAAGCTC TGAGAGAGGG AAAGGAAATG 
GTCATGGGAT TAAATGTCTT TGATCCACAC ACTTCACATT CTTGGCTCTG TGATGGAAGG 
CTGCTGTGCC TTCATGATCC AAGCAACAAG AATAATTGGA AGATATTCAG GGAGTGTTGG 
AAACAGGGTC AGCCTGTACT TGTTTCTGGA GTACAGAAAA AGCTAAAGTC TGAACTTTGG 
AAACCTGAAG CTTTTAGCGT TGAGTTTGGA GACCAAGATG TGGATTTGGT GAACTGTAGA 
AATTGTGCTA TCATTTCTGA TGTGAAAGTT CGTGATTTTT GGGATGGCTT TGCAGTCATT 
GAAAAGAGGT TAAGAGCAGA AGATGGAAGT CCAATGGTCC TCAAGCTAAA AGACTGGCCA 
CCTGGAGAAG ACTTTCGGGA TATGATGCCC ACTCGATTTC AAGACTTGAT GGATAATCTT 
CCACTACCAG AATACACAAA GAGAGACGGC AGGCTCAATT TGGCTTCAAG ATTGCCTAGT 
TATTTTGTAC GACCAGATTT AGGGCCTAAA ATGTACAATG CTTATGGTTT AATTACTGCT 
GAAGACCGGC GCGTAGGTAC TACTAACCTA CATTTGGATG TGTCTGATGC AGTTAATGTT 
ATGGTATATG TGGGGATTCC AGTAGGGGAA AGCAGTCAAA ATGAAGAGGT TTTAAGAACG 
ATTGAGGAGG GTGATGCTGA TGAAGTAACA AAAAAACGTA TTCATGATGG AAAGGAGAAG 
CCTGGTGCTT TGTGGCATAT TTATGCTGCA AAGGACGCAG AGAAGATTAG AGAGCTACTC 
CGCAAGGTAG GTGATGAGCA AGGACAAGAA AACCCTCCAG ACCATGATCC AATCCATGAC 
CAAAGCTGGT ATTTAGACCA GGTACTGCGT AAGCGGCTGT ATGATGAATA TGGTGTACAA 
GGCTGGGCTA TTGTACAGTT CTTGGGAGAT GCTGTGTTCA TTCCAGCTGG TGCACCCCAT 
CAGGTGCACA ATTTGTACAG TTGTATAAAG GTAGCAGAGG ACTTTGTTTC TCCAGAACAT 
GTAAAGCATT GCTTCCGCCT GACGCAGGAA TTCAGGCATT TGTCAAACAC TCACACGAAC 
CATGAGGATA AATTACAGGT GAAGAATATT ATTTACCATG CTGTAAAGGA TGCTGTTGGA 
ACACTAAAGG CTTTTGAGTG TAGATTGGTC AGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_002936848.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936894.1 Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa07678
Clone ID Related Accession (Same CDS sequence)	XM_002936848.4 , XM_002936848.5
Accession Version	XM_002936848.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936848.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTCC CGGTTTCCCC GGTGGGGAAG AGGCTTCGGT TTCTATTTGA CGAATCGGCC 
TCTGGGCGGG AGGGGGCAAC CACTGAACCG TCCACTCCGC TGGGGAAGCG GGCCTGGCAG 
GGCGGGACGG TGCGGGGCCT GAAAGGAGCG GTGCCCGAAG AGCTAGCGAT TTATGTGGAG 
TTTGATGAGA AAAGTTACAA GTCAAATTCT TGGGTGCAGG TTCATGGGGA GGATGTGTTG 
GTGATCCTGA TAGAAGGAGC TTTGGTTTGG TCTCCAAGAA AAGAGCCTAT GTTTATCCAA 
GGAAGCAAAT TATGTCCAAC TCATTGGCCA GCTTTAACCT TTACTCCACT GGTGGACAAG 
TTGGGCTTAG GGACTTTAAT TCCAATAGAA TTTCTTTTGG AAGAGGTTCT GCATTTCCTA 
CCAGATGCCA GCTCTCTGAA ACTTTATCAG CAAATGGGAG CAGACAGTCA TAAACTGGTT 
TTGAAAGAGC AGCCTTTGTT AAAGGATGCA GTGACTTCTG TGATCAATTC AAGGAAAACA 
CAAGATCTCA TTTTAAGAGG TATTTGCAGT ATTCAAGGCC AGGAAGTTAA GGTTTATCAA 
CCAGATACAG AAGTGCCATG GTCTTGTGGA GTTGTGACCC ATCAGGATCT TATTACACGC 
ATGATAAATG TATCAATGAA TGAGGGAAAT GAGATCAAGT CTATTGATCC AAAATTAATT 
CATGTGGTGC TTACTGACGA CACTTTAATA CAACCTGAGG AGGCTACAAA AGCAGGTAAA 
GCATCTAAAT CAAAGAAAAA AAAGGAGAAT ACAGATGCTC GCAGAAGAAA ATTACCTTCA 
GATGCTGAAA ATGATTCTGC TGGAAAAAAG CAGAAAGGCG ATAAGAAAGA TGTGGACAGT 
AATGGAAATG ATGGCAGTGA AGCTAATAGA GGAGTTTGGA AAGCAGGCAG CACTGGAGAA 
GCTACACCAG ATTCAAGATC TAAGCAAATG TTACACTTTG TTCCTCAAAT TAATCGCAAT 
ATCCGATTTG CAACCTACAC CAAAGAGAAT GGACGCACAT TAGTGGTTCA AGATCAGCCT 
GTAAATGATG GAACCTCATT AGCTTTTCCA ACTTTTTCTG GAATGACTAA AGGTATATTG 
TCTGGTGAAA GTGTTGGGAA CTCTGAGAGT GTAACTTCGA GTGGTACTAC TCCTACCACA 
GTACGAATCT CTGATACTAG TCTCTCTGCT TTGACTGGAG AAGAAAAGAA GCCAAACTGG 
TCCCTTGGAG AGAACCTCAG AAAATCACTG CTGACCAAGA GTGGATTTGG CCCATCACTC 
TCTCAGCCAT TATTTGGGAC TGATAAAAAC CTATCTAATG GTGCACTTCA AGAGAACAAA 
GCTACTGGGT TTCAGTTTGG TGCAAATAGT ATTCAGGAGC CTCCTAAGGA AACAGATTTA 
TCAAAAAATC TATTTTTTCA GTGTATGTCA CAAAATATCT CCCCAAGTAC CTATTTTACT 
TCCATATCAG AGAGTGCTAC TAGTGCTCCA GAAGAGCAAC CTAACAAAGA ACTTCCAAAG 
CAAAATAAGG AGGGACTGGC TTCTGGTTTC GGAAAACTTA AACCCCCAGT GACACTGGAA 
AGTAAAGCAA ATTCAAAGAT GTCCTTTTCT GAATCAATGC CAAAACTAAC ACCTGCCTTC 
CCTGGGAAAG TTGTAAGTGC ACGACCTGCA GAGACTCATG AAAATCTGTT TCTTCAGCCC 
CCAAAACTTT CAAGGGAAGA ACCTTCAAAT CCCTTCTTGG CTTTTTCAGA GAAAAAAGAC 
ATTTCACCAT TTGGCTTTTC TTCTCAAACA CCTCAAAGTT CCTTTTCTAG TGCTCTCCCT 
TCTGCTTGGA CAGAACCACG CAAGTTAGAT GACTCAGGGG CTTTGAAGAA ATCTTTGTTT 
CTTTCTGTGG AATCTCCAAA AAGATTGCCT GTTTCTCCTG GTTCATCAAT CAGTGAAACT 
GTACAAAGTT CTGCCTTTCT TGGAAATGGG CGTTCCAGCT CACCAGCAAA CAGCTTAAGT 
CAGCCTATTG AAATGCCTAC ATTGTCATCT AGCCCTACTG AGGATAAGCC TGCAATTGGA 
TCTGGACAGA TAGACAATCC GTTATTAAGA ACCTTTACAG TACTCTCAAG TAGGCTCCAA 
CCTGGTGTCC ACTGCTCCTC AGATTACCCT CAAGAAAACA AAGCAGCTTT TGAATCTGTT 
AACAGATTTA CCCTAGATGA ACGAAGTAGG ACATCAAAAC AAGATTCTGA CTCCAGCACC 
AACAGTGATT TAAGTGATCT AAGTGATTCT GAGGAGCAGC TGAATTCAAA GATTGGCTAT 
AGAGGAATAC CGGAACATCT GGTTGGAAGA TTAGGGCATA ATGGAGAACG TAACGTTGAT 
TTGTTAATGG CAAAAGGTAG AGGAAAACAG CCTTCAAAAG GCAGACCAAG AACAGCTCCG 
CTAAAGGTTG GTCAATCCGT TTTGAAAGAC ATGAGCAAAG TAAGAAAGTT AAAACAGTCT 
GGAGAGCCTT TTCTTCAAGA TGGGTCTTGT ATTAATGTTG CGCCACATTT GCACAAGTGC 
CGTGAATGCA GGCTAGAAAG ATACCGCAAG TTCAAAGAGC AGGATCAGGA CGATTCAACT 
GTAGCTTGTC GATTCTTTCA TTTTCGCAGG CTGGCTTTTA CCAGGAAAGG TATTCTTCGT 
GTTGAGGGCT TCTTAAGTCC CCAGCAAAGT GATGCTGAAG CAATGCGCCT CTGGGTTCCT 
ATTTCTGTTC CAGCGGACGG CATTGATTTG GAGACCTCAA AATATATTCT AGCTAATGTT 
GGAGATCAGT TTTGCCAGTT GGTCATGTCT GAAAAAGAAG CCATGATGAT GGTGGAACCC 
CAGCAAAAGG TAGCATGGAA GAGAGCTGTA CGTGGTGTCA GGGAGATGTG TGATGTATGT 
GAGACGACAC TCTTCAACAT TCACTGGGTT TGTCGCAAGT GTGGCTTTGG CGTATGTCTT 
GATTGTTATC GGCTCCGAAA AAATCGACCT CGCAGAGAAG ATGAAATTGG TGATGAAGAA 
GTCTTTGCGT GGCTAAAATG TGCGAAAGGA CAACCTCACG AACCAGAGAA TCTTATGCCT 
ACCCAGATTA TTCCAGGCAC TGCTCTTTAC AACATTGGGG ATATGGTCCA TGCAGCACGA 
GGGAAATGGG GCATTAAAGC CAATTGTCCA TGTATTAACA GACAGAATAA ATCTGTATTA 
AGGCCAGCAG TCACAAATGG AATGTCTCAG CTGCCTAATG CTAATAATGC TGCTGCTGAT 
ACAACTACTA CCGCAGATGA GGGGATTGAA AATAAATCAG ACCAGCCTTT AAAGAAAGAG 
GAAACAGAAA ATGAGGCATC GTCCAATAAT GATATCGATA TTGCTGCAAA TAGCAGCACG 
GATAGCAAAA ACAGCCGTCC TCAATGCCCG GAATCTACTC CTTCCTCTGC CTTACATTGG 
CTAGCAGACC TTGCAGCCCA GAAAGCAAAA GAAGAGACAA AAGACAATGG AACACCACAA 
TCAACACCCA GCAAAGAGTC AAGTCCACCT TCTGGACTGG ACCCATTAAA TGCAAATGTT 
AAGACTTCAT CTCCAAAACT TTTTAATAGC CTGTTATTGG GACCAGCTGC TTCAAATACT 
AAGACAGAAG GCTCCAGTCT TAGAGATCTG CTCCATTCTG GCCAAGGAAA ACTTTCTCAA 
GGAAATATGG AAGCAGGAAT TCCATTCCCA CCTGTCTTTT CCACAAGTTC AGCAAAGGGG 
AAAACAAGTT TACCAAATTT CCTTGATCAC ATTATTGCTT CTGTGGTCGA AAATAAAAAG 
ACTTCAGATG CCGCTAAAAG AGCCAGTAGC TCAACGGAAG CTCTGAGAGA GGGAAAGGAA 
ATGGTCATGG GATTAAATGT CTTTGATCCA CACACTTCAC ATTCTTGGCT CTGTGATGGA 
AGGCTGCTGT GCCTTCATGA TCCAAGCAAC AAGAATAATT GGAAGATATT CAGGGAGTGT 
TGGAAACAGG GTCAGCCTGT ACTTGTTTCT GGAGTACAGA AAAAGCTAAA GTCTGAACTT 
TGGAAACCTG AAGCTTTTAG CGTTGAGTTT GGAGACCAAG ATGTGGATTT GGTGAACTGT 
AGAAATTGTG CTATCATTTC TGATGTGAAA GTTCGTGATT TTTGGGATGG CTTTGCAGTC 
ATTGAAAAGA GGTTAAGAGC AGAAGATGGA AGTCCAATGG TCCTCAAGCT AAAAGACTGG 
CCACCTGGAG AAGACTTTCG GGATATGATG CCCACTCGAT TTCAAGACTT GATGGATAAT 
CTTCCACTAC CAGAATACAC AAAGAGAGAC GGCAGGCTCA ATTTGGCTTC AAGATTGCCT 
AGTTATTTTG TACGACCAGA TTTAGGGCCT AAAATGTACA ATGCTTATGG TTTAATTACT 
GCTGAAGACC GGCGCGTAGG TACTACTAAC CTACATTTGG ATGTGTCTGA TGCAGTTAAT 
GTTATGGTAT ATGTGGGGAT TCCAGTAGGG GAAAGCAGTC AAAATGAAGA GGTTTTAAGA 
ACGATTGAGG AGGGTGATGC TGATGAAGTA ACAAAAAAAC GTATTCATGA TGGAAAGGAG 
AAGCCTGGTG CTTTGTGGCA TATTTATGCT GCAAAGGACG CAGAGAAGAT TAGAGAGCTA 
CTCCGCAAGG TAGGTGATGA GCAAGGACAA GAAAACCCTC CAGACCATGA TCCAATCCAT 
GACCAAAGCT GGTATTTAGA CCAGGTACTG CGTAAGCGGC TGTATGATGA ATATGGTGTA 
CAAGGCTGGG CTATTGTACA GTTCTTGGGA GATGCTGTGT TCATTCCAGC TGGTGCACCC 
CATCAGGTGC ACAATTTGTA CAGTTGTATA AAGGTAGCAG AGGACTTTGT TTCTCCAGAA 
CATGTAAAGC ATTGCTTCCG CCTGACGCAG GAATTCAGGC ATTTGTCAAA CACTCACACG 
AACCATGAGG ATAAATTACA GGTGAAGAAT ATTATTTACC ATGCTGTAAA GGATGCTGTT 
GGAACACTAA AGGCTTTTGA GTGTAGATTG GTCAGAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936894.1
CDS	814..5895
Translation	MAVPVSPVGKRLRFLFDESASGREGATTEPSTPLGKRAWQGGTVRGLKGAVPEELAIYVEFDEKSYKSNSWVQVHGEDVLVILIEGALVWSPRKEPMFIQGSKLCPTHWPALTFTPLVDKLGLGTLIPIEFLLEEVLHFLPDASSLKLYQQMGADSHKLVLKEQPLLKDAVTSVINSRKTQDLILRGICSIQGQEVKVYQPDTEVPWSCGVVTHQDLITRMINVSMNEGNEIKSIDPKLIHVVLTDDTLIQPEEATKAGKASKSKKKKENTDARRRKLPSDAENDSAGKKQKGDKKDVDSNGNDGSEANRGVWKAGSTGEATPDSRSKQMLHFVPQINRNIRFATYTKENGRTLVVQDQPVNDGTSLAFPTFSGMTKGILSGESVGNSESVTSSGTTPTTVRISDTSLSALTGEEKKPNWSLGENLRKSLLTKSGFGPSLSQPLFGTDKNLSNGALQENKATGFQFGANSIQEPPKETDLSKNLFFQCMSQNISPSTYFTSISESATSAPEEQPNKELPKQNKEGLASGFGKLKPPVTLESKANSKMSFSESMPKLTPAFPGKVVSARPAETHENLFLQPPKLSREEPSNPFLAFSEKKDISPFGFSSQTPQSSFSSALPSAWTEPRKLDDSGALKKSLFLSVESPKRLPVSPGSSISETVQSSAFLGNGRSSSPANSLSQPIEMPTLSSSPTEDKPAIGSGQIDNPLLRTFTVLSSRLQPGVHCSSDYPQENKAAFESVNRFTLDERSRTSKQDSDSSTNSDLSDLSDSEEQLNSKIGYRGIPEHLVGRLGHNGERNVDLLMAKGRGKQPSKGRPRTAPLKVGQSVLKDMSKVRKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQDQDDSTVACRFFHFRRLAFTRKGILRVEGFLSPQQSDAEAMRLWVPISVPADGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPQQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPRREDEIGDEEVFAWLKCAKGQPHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCINRQNKSVLRPAVTNGMSQLPNANNAAADTTTTADEGIENKSDQPLKKEETENEASSNNDIDIAANSSTDSKNSRPQCPESTPSSALHWLADLAAQKAKEETKDNGTPQSTPSKESSPPSGLDPLNANVKTSSPKLFNSLLLGPAASNTKTEGSSLRDLLHSGQGKLSQGNMEAGIPFPPVFSTSSAKGKTSLPNFLDHIIASVVENKKTSDAAKRASSSTEALREGKEMVMGLNVFDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVQKKLKSELWKPEAFSVEFGDQDVDLVNCRNCAIISDVKVRDFWDGFAVIEKRLRAEDGSPMVLKLKDWPPGEDFRDMMPTRFQDLMDNLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPVGESSQNEEVLRTIEEGDADEVTKKRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGDEQGQENPPDHDPIHDQSWYLDQVLRKRLYDEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAFECRLVRT

Target ORF information:

RefSeq Version	XM_002936848.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936848.4

ORF Insert Sequence:

ATGGCGGTCC CGGTTTCCCC GGTGGGGAAG AGGCTTCGGT TTCTATTTGA CGAATCGGCC 
TCTGGGCGGG AGGGGGCAAC CACTGAACCG TCCACTCCGC TGGGGAAGCG GGCCTGGCAG 
GGCGGGACGG TGCGGGGCCT GAAAGGAGCG GTGCCCGAAG AGCTAGCGAT TTATGTGGAG 
TTTGATGAGA AAAGTTACAA GTCAAATTCT TGGGTGCAGG TTCATGGGGA GGATGTGTTG 
GTGATCCTGA TAGAAGGAGC TTTGGTTTGG TCTCCAAGAA AAGAGCCTAT GTTTATCCAA 
GGAAGCAAAT TATGTCCAAC TCATTGGCCA GCTTTAACCT TTACTCCACT GGTGGACAAG 
TTGGGCTTAG GGACTTTAAT TCCAATAGAA TTTCTTTTGG AAGAGGTTCT GCATTTCCTA 
CCAGATGCCA GCTCTCTGAA ACTTTATCAG CAAATGGGAG CAGACAGTCA TAAACTGGTT 
TTGAAAGAGC AGCCTTTGTT AAAGGATGCA GTGACTTCTG TGATCAATTC AAGGAAAACA 
CAAGATCTCA TTTTAAGAGG TATTTGCAGT ATTCAAGGCC AGGAAGTTAA GGTTTATCAA 
CCAGATACAG AAGTGCCATG GTCTTGTGGA GTTGTGACCC ATCAGGATCT TATTACACGC 
ATGATAAATG TATCAATGAA TGAGGGAAAT GAGATCAAGT CTATTGATCC AAAATTAATT 
CATGTGGTGC TTACTGACGA CACTTTAATA CAACCTGAGG AGGCTACAAA AGCAGGTAAA 
GCATCTAAAT CAAAGAAAAA AAAGGAGAAT ACAGATGCTC GCAGAAGAAA ATTACCTTCA 
GATGCTGAAA ATGATTCTGC TGGAAAAAAG CAGAAAGGCG ATAAGAAAGA TGTGGACAGT 
AATGGAAATG ATGGCAGTGA AGCTAATAGA GGAGTTTGGA AAGCAGGCAG CACTGGAGAA 
GCTACACCAG ATTCAAGATC TAAGCAAATG TTACACTTTG TTCCTCAAAT TAATCGCAAT 
ATCCGATTTG CAACCTACAC CAAAGAGAAT GGACGCACAT TAGTGGTTCA AGATCAGCCT 
GTAAATGATG GAACCTCATT AGCTTTTCCA ACTTTTTCTG GAATGACTAA AGGTATATTG 
TCTGGTGAAA GTGTTGGGAA CTCTGAGAGT GTAACTTCGA GTGGTACTAC TCCTACCACA 
GTACGAATCT CTGATACTAG TCTCTCTGCT TTGACTGGAG AAGAAAAGAA GCCAAACTGG 
TCCCTTGGAG AGAACCTCAG AAAATCACTG CTGACCAAGA GTGGATTTGG CCCATCACTC 
TCTCAGCCAT TATTTGGGAC TGATAAAAAC CTATCTAATG GTGCACTTCA AGAGAACAAA 
GCTACTGGGT TTCAGTTTGG TGCAAATAGT ATTCAGGAGC CTCCTAAGGA AACAGATTTA 
TCAAAAAATC TATTTTTTCA GTGTATGTCA CAAAATATCT CCCCAAGTAC CTATTTTACT 
TCCATATCAG AGAGTGCTAC TAGTGCTCCA GAAGAGCAAC CTAACAAAGA ACTTCCAAAG 
CAAAATAAGG AGGGACTGGC TTCTGGTTTC GGAAAACTTA AACCCCCAGT GACACTGGAA 
AGTAAAGCAA ATTCAAAGAT GTCCTTTTCT GAATCAATGC CAAAACTAAC ACCTGCCTTC 
CCTGGGAAAG TTGTAAGTGC ACGACCTGCA GAGACTCATG AAAATCTGTT TCTTCAGCCC 
CCAAAACTTT CAAGGGAAGA ACCTTCAAAT CCCTTCTTGG CTTTTTCAGA GAAAAAAGAC 
ATTTCACCAT TTGGCTTTTC TTCTCAAACA CCTCAAAGTT CCTTTTCTAG TGCTCTCCCT 
TCTGCTTGGA CAGAACCACG CAAGTTAGAT GACTCAGGGG CTTTGAAGAA ATCTTTGTTT 
CTTTCTGTGG AATCTCCAAA AAGATTGCCT GTTTCTCCTG GTTCATCAAT CAGTGAAACT 
GTACAAAGTT CTGCCTTTCT TGGAAATGGG CGTTCCAGCT CACCAGCAAA CAGCTTAAGT 
CAGCCTATTG AAATGCCTAC ATTGTCATCT AGCCCTACTG AGGATAAGCC TGCAATTGGA 
TCTGGACAGA TAGACAATCC GTTATTAAGA ACCTTTACAG TACTCTCAAG TAGGCTCCAA 
CCTGGTGTCC ACTGCTCCTC AGATTACCCT CAAGAAAACA AAGCAGCTTT TGAATCTGTT 
AACAGATTTA CCCTAGATGA ACGAAGTAGG ACATCAAAAC AAGATTCTGA CTCCAGCACC 
AACAGTGATT TAAGTGATCT AAGTGATTCT GAGGAGCAGC TGAATTCAAA GATTGGCTAT 
AGAGGAATAC CGGAACATCT GGTTGGAAGA TTAGGGCATA ATGGAGAACG TAACGTTGAT 
TTGTTAATGG CAAAAGGTAG AGGAAAACAG CCTTCAAAAG GCAGACCAAG AACAGCTCCG 
CTAAAGGTTG GTCAATCCGT TTTGAAAGAC ATGAGCAAAG TAAGAAAGTT AAAACAGTCT 
GGAGAGCCTT TTCTTCAAGA TGGGTCTTGT ATTAATGTTG CGCCACATTT GCACAAGTGC 
CGTGAATGCA GGCTAGAAAG ATACCGCAAG TTCAAAGAGC AGGATCAGGA CGATTCAACT 
GTAGCTTGTC GATTCTTTCA TTTTCGCAGG CTGGCTTTTA CCAGGAAAGG TATTCTTCGT 
GTTGAGGGCT TCTTAAGTCC CCAGCAAAGT GATGCTGAAG CAATGCGCCT CTGGGTTCCT 
ATTTCTGTTC CAGCGGACGG CATTGATTTG GAGACCTCAA AATATATTCT AGCTAATGTT 
GGAGATCAGT TTTGCCAGTT GGTCATGTCT GAAAAAGAAG CCATGATGAT GGTGGAACCC 
CAGCAAAAGG TAGCATGGAA GAGAGCTGTA CGTGGTGTCA GGGAGATGTG TGATGTATGT 
GAGACGACAC TCTTCAACAT TCACTGGGTT TGTCGCAAGT GTGGCTTTGG CGTATGTCTT 
GATTGTTATC GGCTCCGAAA AAATCGACCT CGCAGAGAAG ATGAAATTGG TGATGAAGAA 
GTCTTTGCGT GGCTAAAATG TGCGAAAGGA CAACCTCACG AACCAGAGAA TCTTATGCCT 
ACCCAGATTA TTCCAGGCAC TGCTCTTTAC AACATTGGGG ATATGGTCCA TGCAGCACGA 
GGGAAATGGG GCATTAAAGC CAATTGTCCA TGTATTAACA GACAGAATAA ATCTGTATTA 
AGGCCAGCAG TCACAAATGG AATGTCTCAG CTGCCTAATG CTAATAATGC TGCTGCTGAT 
ACAACTACTA CCGCAGATGA GGGGATTGAA AATAAATCAG ACCAGCCTTT AAAGAAAGAG 
GAAACAGAAA ATGAGGCATC GTCCAATAAT GATATCGATA TTGCTGCAAA TAGCAGCACG 
GATAGCAAAA ACAGCCGTCC TCAATGCCCG GAATCTACTC CTTCCTCTGC CTTACATTGG 
CTAGCAGACC TTGCAGCCCA GAAAGCAAAA GAAGAGACAA AAGACAATGG AACACCACAA 
TCAACACCCA GCAAAGAGTC AAGTCCACCT TCTGGACTGG ACCCATTAAA TGCAAATGTT 
AAGACTTCAT CTCCAAAACT TTTTAATAGC CTGTTATTGG GACCAGCTGC TTCAAATACT 
AAGACAGAAG GCTCCAGTCT TAGAGATCTG CTCCATTCTG GCCAAGGAAA ACTTTCTCAA 
GGAAATATGG AAGCAGGAAT TCCATTCCCA CCTGTCTTTT CCACAAGTTC AGCAAAGGGG 
AAAACAAGTT TACCAAATTT CCTTGATCAC ATTATTGCTT CTGTGGTCGA AAATAAAAAG 
ACTTCAGATG CCGCTAAAAG AGCCAGTAGC TCAACGGAAG CTCTGAGAGA GGGAAAGGAA 
ATGGTCATGG GATTAAATGT CTTTGATCCA CACACTTCAC ATTCTTGGCT CTGTGATGGA 
AGGCTGCTGT GCCTTCATGA TCCAAGCAAC AAGAATAATT GGAAGATATT CAGGGAGTGT 
TGGAAACAGG GTCAGCCTGT ACTTGTTTCT GGAGTACAGA AAAAGCTAAA GTCTGAACTT 
TGGAAACCTG AAGCTTTTAG CGTTGAGTTT GGAGACCAAG ATGTGGATTT GGTGAACTGT 
AGAAATTGTG CTATCATTTC TGATGTGAAA GTTCGTGATT TTTGGGATGG CTTTGCAGTC 
ATTGAAAAGA GGTTAAGAGC AGAAGATGGA AGTCCAATGG TCCTCAAGCT AAAAGACTGG 
CCACCTGGAG AAGACTTTCG GGATATGATG CCCACTCGAT TTCAAGACTT GATGGATAAT 
CTTCCACTAC CAGAATACAC AAAGAGAGAC GGCAGGCTCA ATTTGGCTTC AAGATTGCCT 
AGTTATTTTG TACGACCAGA TTTAGGGCCT AAAATGTACA ATGCTTATGG TTTAATTACT 
GCTGAAGACC GGCGCGTAGG TACTACTAAC CTACATTTGG ATGTGTCTGA TGCAGTTAAT 
GTTATGGTAT ATGTGGGGAT TCCAGTAGGG GAAAGCAGTC AAAATGAAGA GGTTTTAAGA 
ACGATTGAGG AGGGTGATGC TGATGAAGTA ACAAAAAAAC GTATTCATGA TGGAAAGGAG 
AAGCCTGGTG CTTTGTGGCA TATTTATGCT GCAAAGGACG CAGAGAAGAT TAGAGAGCTA 
CTCCGCAAGG TAGGTGATGA GCAAGGACAA GAAAACCCTC CAGACCATGA TCCAATCCAT 
GACCAAAGCT GGTATTTAGA CCAGGTACTG CGTAAGCGGC TGTATGATGA ATATGGTGTA 
CAAGGCTGGG CTATTGTACA GTTCTTGGGA GATGCTGTGT TCATTCCAGC TGGTGCACCC 
CATCAGGTGC ACAATTTGTA CAGTTGTATA AAGGTAGCAG AGGACTTTGT TTCTCCAGAA 
CATGTAAAGC ATTGCTTCCG CCTGACGCAG GAATTCAGGC ATTTGTCAAA CACTCACACG 
AACCATGAGG ATAAATTACA GGTGAAGAAT ATTATTTACC ATGCTGTAAA GGATGCTGTT 
GGAACACTAA AGGCTTTTGA GTGTAGATTG GTCAGAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_004912889.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912946.1 Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.

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CloneID	OXa07679
Clone ID Related Accession (Same CDS sequence)	XM_004912889.3 , XM_004912889.4
Accession Version	XM_004912889.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTCC CGGTTTCCCC GGTGGGGAAG AGGCTTCGGT TTCTATTTGA CGAATCGGCC 
TCTGGGCGGG AGGGGGCAAC CACTGAACCG TCCACTCCGC TGGGGAAGCG GGCCTGGCAG 
GGCGGGACGG TGCGGGGCCT GAAAGGAGCG GTGCCCGAAG AGCTAGCGAT TTATGTGGAG 
TTTGATGAGA AAAGTTACAA GTCAAATTCT TGGGTGCAGG TTCATGGGGA GGATGTGTTG 
GTGATCCTGA TAGAAGGAGC TTTGGTTTGG TCTCCAAGAA AAGAGCCTAT GTTTATCCAA 
GGAAGCAAAT TATGTCCAAC TCATTGGCCA GCTTTAACCT TTACTCCACT GGTGGACAAG 
TTGGGCTTAG GGACTTTAAT TCCAATAGAA TTTCTTTTGG AAGAGGTTCT GCATTTCCTA 
CCAGATGCCA GCTCTCTGAA ACTTTATCAG CAAATGGGAG CAGACAGTCA TAAACTGGTT 
TTGAAAGAGC AGCCTTTGTT AAAGGATGCA GTGACTTCTG TGATCAATTC AAGGAAAACA 
CAAGATCTCA TTTTAAGAGG TATTTGCAGT ATTCAAGGCC AGGAAGTTAA GGTTTATCAA 
CCAGATACAG AAGTGCCATG GTCTTGTGGA GTTGTGACCC ATCAGGATCT TATTACACGC 
ATGATAAATG TATCAATGAA TGAGGGAAAT GAGATCAAGT CTATTGATCC AAAATTAATT 
CATGTGGTGC TTACTGACGA CACTTTAATA CAACCTGAGG AGGCTACAAA AGCAGGTAAA 
GCATCTAAAT CAAAGAAAAA AAAGGAGAAT ACAGATGCTC GCAGAAGAAA ATTACCTTCA 
GATGCTGAAA ATGATTCTGC TGGAAAAAAG CAGAAAGGCG ATAAGAAAGA TGTGGACAGT 
AATGGAAATG ATGGCAGTGA AGCTAATAGA GGAGTTTGGA AAGCAGGCAG CACTGGAGAA 
GCTACACCAG ATTCAAGATC TAAGCAAATG TTACACTTTG TTCCTCAAAT TAATCGCAAT 
ATCCGATTTG CAACCTACAC CAAAGAGAAT GGACGCACAT TAGTGGTTCA AGATCAGCCT 
GTAAATGATG GAACCTCATT AGCTTTTCCA ACTTTTTCTG GAATGACTAA AGGTATATTG 
TCTGGTGAAA GTGTTGGGAA CTCTGAGAGT GTAACTTCGA GTGGTACTAC TCCTACCACA 
GTACGAATCT CTGATACTAG TCTCTCTGCT TTGACTGGAG AAGAAAAGAA GCCAAACTGG 
TCCCTTGGAG AGAACCTCAG AAAATCACTG CTGACCAAGA GTGGATTTGG CCCATCACTC 
TCTCAGCCAT TATTTGGGAC TGATAAAAAC CTATCTAATG GTGCACTTCA AGAGAACAAA 
GCTACTGGGT TTCAGTTTGG TGCAAATAGT ATTCAGGAGC CTCCTAAGGA AACAGATTTA 
TCAAAAAATC TATTTTTTCA GTGTATGTCA CAAAATATCT CCCCAAGTAC CTATTTTACT 
TCCATATCAG AGAGTGCTAC TAGTGCTCCA GAAGAGCAAC CTAACAAAGA ACTTCCAAAG 
CAAAATAAGG AGGGACTGGC TTCTGGTTTC GGAAAACTTA AACCCCCAGT GACACTGGAA 
AGTAAAGCAA ATTCAAAGAT GTCCTTTTCT GAATCAATGC CAAAACTAAC ACCTGCCTTC 
CCTGGGAAAG TTGTAAGTGC ACGACCTGCA GAGACTCATG AAAATCTGTT TCTTCAGCCC 
CCAAAACTTT CAAGGGAAGA ACCTTCAAAT CCCTTCTTGG CTTTTTCAGA GAAAAAAGAC 
ATTTCACCAT TTGGCTTTTC TTCTCAAACA CCTCAAAGTT CCTTTTCTAG TGCTCTCCCT 
TCTGCTTGGA CAGAACCACG CAAGTTAGAT GACTCAGGGG CTTTGAAGAA ATCTTTGTTT 
CTTTCTGTGG AATCTCCAAA AAGATTGCCT GTTTCTCCTG GTTCATCAAT CAGTGAAACT 
GTACAAAGTT CTGCCTTTCT TGGAAATGGG CGTTCCAGCT CACCAGCAAA CAGCTTAAGT 
CAGCCTATTG AAATGCCTAC ATTGTCATCT AGCCCTACTG AGGATAAGCC TGCAATTGGA 
TCTGGACAGA TAGACAATCC GTTATTAAGA ACCTTTACAG TACTCTCAAG TAGGCTCCAA 
CCTGGTGTCC ACTGCTCCTC AGATTACCCT CAAGAAAACA AAGCAGCTTT TGAATCTGTT 
AACAGATTTA CCCTAGATGA ACGAAGTAGG ACATCAAAAC AAGATTCTGA CTCCAGCACC 
AACAGTGATT TAAGTGATCT AAGTGATTCT GAGGAGCAGC TGAATTCAAA GATTGGCTAT 
AGAGGAATAC CGGAACATCT GGTTGGAAGA TTAGGGCATA ATGGAGAACG TAACGTTGAT 
TTGTTAATGG CAAAAGGTAG AGGAAAACAG CCTTCAAAAG GCAGACCAAG AACAGCTCCG 
CTAAAGGTTG GTCAATCCGT TTTGAAAGAC ATGAGCAAAG TAAGAAAGTT AAAACAGTCT 
GGAGAGCCTT TTCTTCAAGA TGGGTCTTGT ATTAATGTTG CGCCACATTT GCACAAGTGC 
CGTGAATGCA GGCTAGAAAG ATACCGCAAG TTCAAAGAGC AGGATCAGGA CGATTCAACT 
GTAGCTTGTC GATTCTTTCA TTTTCGCAGG CTGGCTTTTA CCAGGAAAGG TATTCTTCGT 
GTTGAGGGCT TCTTAAGTCC CCAGCAAAGT GATGCTGAAG CAATGCGCCT CTGGGTTCCT 
ATTTCTGTTC CAGCGGACGG CATTGATTTG GAGACCTCAA AATATATTCT AGCTAATGTT 
GGAGATCAGT TTTGCCAGTT GGTCATGTCT GAAAAAGAAG CCATGATGAT GGTGGAACCC 
CAGCAAAAGG TAGCATGGAA GAGAGCTGTA CGTGGTGTCA GGGAGATGTG TGATGTATGT 
GAGACGACAC TCTTCAACAT TCACTGGGTT TGTCGCAAGT GTGGCTTTGG CGTATGTCTT 
GATTGTTATC GGCTCCGAAA AAATCGACCT CGCAGAGATG AAATTGGTGA TGAAGAAGTC 
TTTGCGTGGC TAAAATGTGC GAAAGGACAA CCTCACGAAC CAGAGAATCT TATGCCTACC 
CAGATTATTC CAGGCACTGC TCTTTACAAC ATTGGGGATA TGGTCCATGC AGCACGAGGG 
AAATGGGGCA TTAAAGCCAA TTGTCCATGT ATTAACAGAC AGAATAAATC TGTATTAAGG 
CCAGCAGTCA CAAATGGAAT GTCTCAGCTG CCTAATGCTA ATAATGCTGC TGCTGATACA 
ACTACTACCG CAGATGAGGG GATTGAAAAT AAATCAGACC AGCCTTTAAA GAAAGAGGAA 
ACAGAAAATG AGGCATCGTC CAATAATGAT ATCGATATTG CTGCAAATAG CAGCACGGAT 
AGCAAAAACA GCCGTCCTCA ATGCCCGGAA TCTACTCCTT CCTCTGCCTT ACATTGGCTA 
GCAGACCTTG CAGCCCAGAA AGCAAAAGAA GAGACAAAAG ACAATGGAAC ACCACAATCA 
ACACCCAGCA AAGAGTCAAG TCCACCTTCT GGACTGGACC CATTAAATGC AAATGTTAAG 
ACTTCATCTC CAAAACTTTT TAATAGCCTG TTATTGGGAC CAGCTGCTTC AAATACTAAG 
ACAGAAGGCT CCAGTCTTAG AGATCTGCTC CATTCTGGCC AAGGAAAACT TTCTCAAGGA 
AATATGGAAG CAGGAATTCC ATTCCCACCT GTCTTTTCCA CAAGTTCAGC AAAGGGGAAA 
ACAAGTTTAC CAAATTTCCT TGATCACATT ATTGCTTCTG TGGTCGAAAA TAAAAAGACT 
TCAGATGCCG CTAAAAGAGC CAGTAGCTCA ACGGAAGCTC TGAGAGAGGG AAAGGAAATG 
GTCATGGGAT TAAATGTCTT TGATCCACAC ACTTCACATT CTTGGCTCTG TGATGGAAGG 
CTGCTGTGCC TTCATGATCC AAGCAACAAG AATAATTGGA AGATATTCAG GGAGTGTTGG 
AAACAGGGTC AGCCTGTACT TGTTTCTGGA GTACAGAAAA AGCTAAAGTC TGAACTTTGG 
AAACCTGAAG CTTTTAGCGT TGAGTTTGGA GACCAAGATG TGGATTTGGT GAACTGTAGA 
AATTGTGCTA TCATTTCTGA TGTGAAAGTT CGTGATTTTT GGGATGGCTT TGCAGTCATT 
GAAAAGAGGT TAAGAGCAGA AGATGGAAGT CCAATGGTCC TCAAGCTAAA AGACTGGCCA 
CCTGGAGAAG ACTTTCGGGA TATGATGCCC ACTCGATTTC AAGACTTGAT GGATAATCTT 
CCACTACCAG AATACACAAA GAGAGACGGC AGGCTCAATT TGGCTTCAAG ATTGCCTAGT 
TATTTTGTAC GACCAGATTT AGGGCCTAAA ATGTACAATG CTTATGGTTT AATTACTGCT 
GAAGACCGGC GCGTAGGTAC TACTAACCTA CATTTGGATG TGTCTGATGC AGTTAATGTT 
ATGGTATATG TGGGGATTCC AGTAGGGGAA AGCAGTCAAA ATGAAGAGGT TTTAAGAACG 
ATTGAGGAGG GTGATGCTGA TGAAGTAACA AAAAAACGTA TTCATGATGG AAAGGAGAAG 
CCTGGTGCTT TGTGGCATAT TTATGCTGCA AAGGACGCAG AGAAGATTAG AGAGCTACTC 
CGCAAGGTAG GTGATGAGCA AGGACAAGAA AACCCTCCAG ACCATGATCC AATCCATGAC 
CAAAGCTGGT ATTTAGACCA GGTACTGCGT AAGCGGCTGT ATGATGAATA TGGTGTACAA 
GGCTGGGCTA TTGTACAGTT CTTGGGAGAT GCTGTGTTCA TTCCAGCTGG TGCACCCCAT 
CAGGTGCACA ATTTGTACAG TTGTATAAAG GTAGCAGAGG ACTTTGTTTC TCCAGAACAT 
GTAAAGCATT GCTTCCGCCT GACGCAGGAA TTCAGGCATT TGTCAAACAC TCACACGAAC 
CATGAGGATA AATTACAGGT GAAGAATATT ATTTACCATG CTGTAAAGGA TGCTGTTGGA 
ACACTAAAGG CTTTTGAGTG TAGATTGGTC AGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912946.1
CDS	816..5894
Translation	MAVPVSPVGKRLRFLFDESASGREGATTEPSTPLGKRAWQGGTVRGLKGAVPEELAIYVEFDEKSYKSNSWVQVHGEDVLVILIEGALVWSPRKEPMFIQGSKLCPTHWPALTFTPLVDKLGLGTLIPIEFLLEEVLHFLPDASSLKLYQQMGADSHKLVLKEQPLLKDAVTSVINSRKTQDLILRGICSIQGQEVKVYQPDTEVPWSCGVVTHQDLITRMINVSMNEGNEIKSIDPKLIHVVLTDDTLIQPEEATKAGKASKSKKKKENTDARRRKLPSDAENDSAGKKQKGDKKDVDSNGNDGSEANRGVWKAGSTGEATPDSRSKQMLHFVPQINRNIRFATYTKENGRTLVVQDQPVNDGTSLAFPTFSGMTKGILSGESVGNSESVTSSGTTPTTVRISDTSLSALTGEEKKPNWSLGENLRKSLLTKSGFGPSLSQPLFGTDKNLSNGALQENKATGFQFGANSIQEPPKETDLSKNLFFQCMSQNISPSTYFTSISESATSAPEEQPNKELPKQNKEGLASGFGKLKPPVTLESKANSKMSFSESMPKLTPAFPGKVVSARPAETHENLFLQPPKLSREEPSNPFLAFSEKKDISPFGFSSQTPQSSFSSALPSAWTEPRKLDDSGALKKSLFLSVESPKRLPVSPGSSISETVQSSAFLGNGRSSSPANSLSQPIEMPTLSSSPTEDKPAIGSGQIDNPLLRTFTVLSSRLQPGVHCSSDYPQENKAAFESVNRFTLDERSRTSKQDSDSSTNSDLSDLSDSEEQLNSKIGYRGIPEHLVGRLGHNGERNVDLLMAKGRGKQPSKGRPRTAPLKVGQSVLKDMSKVRKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQDQDDSTVACRFFHFRRLAFTRKGILRVEGFLSPQQSDAEAMRLWVPISVPADGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPQQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPRRDEIGDEEVFAWLKCAKGQPHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCINRQNKSVLRPAVTNGMSQLPNANNAAADTTTTADEGIENKSDQPLKKEETENEASSNNDIDIAANSSTDSKNSRPQCPESTPSSALHWLADLAAQKAKEETKDNGTPQSTPSKESSPPSGLDPLNANVKTSSPKLFNSLLLGPAASNTKTEGSSLRDLLHSGQGKLSQGNMEAGIPFPPVFSTSSAKGKTSLPNFLDHIIASVVENKKTSDAAKRASSSTEALREGKEMVMGLNVFDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVQKKLKSELWKPEAFSVEFGDQDVDLVNCRNCAIISDVKVRDFWDGFAVIEKRLRAEDGSPMVLKLKDWPPGEDFRDMMPTRFQDLMDNLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPVGESSQNEEVLRTIEEGDADEVTKKRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGDEQGQENPPDHDPIHDQSWYLDQVLRKRLYDEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAFECRLVRT

Target ORF information:

RefSeq Version	XM_004912889.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912889.3

ORF Insert Sequence:

ATGGCGGTCC CGGTTTCCCC GGTGGGGAAG AGGCTTCGGT TTCTATTTGA CGAATCGGCC 
TCTGGGCGGG AGGGGGCAAC CACTGAACCG TCCACTCCGC TGGGGAAGCG GGCCTGGCAG 
GGCGGGACGG TGCGGGGCCT GAAAGGAGCG GTGCCCGAAG AGCTAGCGAT TTATGTGGAG 
TTTGATGAGA AAAGTTACAA GTCAAATTCT TGGGTGCAGG TTCATGGGGA GGATGTGTTG 
GTGATCCTGA TAGAAGGAGC TTTGGTTTGG TCTCCAAGAA AAGAGCCTAT GTTTATCCAA 
GGAAGCAAAT TATGTCCAAC TCATTGGCCA GCTTTAACCT TTACTCCACT GGTGGACAAG 
TTGGGCTTAG GGACTTTAAT TCCAATAGAA TTTCTTTTGG AAGAGGTTCT GCATTTCCTA 
CCAGATGCCA GCTCTCTGAA ACTTTATCAG CAAATGGGAG CAGACAGTCA TAAACTGGTT 
TTGAAAGAGC AGCCTTTGTT AAAGGATGCA GTGACTTCTG TGATCAATTC AAGGAAAACA 
CAAGATCTCA TTTTAAGAGG TATTTGCAGT ATTCAAGGCC AGGAAGTTAA GGTTTATCAA 
CCAGATACAG AAGTGCCATG GTCTTGTGGA GTTGTGACCC ATCAGGATCT TATTACACGC 
ATGATAAATG TATCAATGAA TGAGGGAAAT GAGATCAAGT CTATTGATCC AAAATTAATT 
CATGTGGTGC TTACTGACGA CACTTTAATA CAACCTGAGG AGGCTACAAA AGCAGGTAAA 
GCATCTAAAT CAAAGAAAAA AAAGGAGAAT ACAGATGCTC GCAGAAGAAA ATTACCTTCA 
GATGCTGAAA ATGATTCTGC TGGAAAAAAG CAGAAAGGCG ATAAGAAAGA TGTGGACAGT 
AATGGAAATG ATGGCAGTGA AGCTAATAGA GGAGTTTGGA AAGCAGGCAG CACTGGAGAA 
GCTACACCAG ATTCAAGATC TAAGCAAATG TTACACTTTG TTCCTCAAAT TAATCGCAAT 
ATCCGATTTG CAACCTACAC CAAAGAGAAT GGACGCACAT TAGTGGTTCA AGATCAGCCT 
GTAAATGATG GAACCTCATT AGCTTTTCCA ACTTTTTCTG GAATGACTAA AGGTATATTG 
TCTGGTGAAA GTGTTGGGAA CTCTGAGAGT GTAACTTCGA GTGGTACTAC TCCTACCACA 
GTACGAATCT CTGATACTAG TCTCTCTGCT TTGACTGGAG AAGAAAAGAA GCCAAACTGG 
TCCCTTGGAG AGAACCTCAG AAAATCACTG CTGACCAAGA GTGGATTTGG CCCATCACTC 
TCTCAGCCAT TATTTGGGAC TGATAAAAAC CTATCTAATG GTGCACTTCA AGAGAACAAA 
GCTACTGGGT TTCAGTTTGG TGCAAATAGT ATTCAGGAGC CTCCTAAGGA AACAGATTTA 
TCAAAAAATC TATTTTTTCA GTGTATGTCA CAAAATATCT CCCCAAGTAC CTATTTTACT 
TCCATATCAG AGAGTGCTAC TAGTGCTCCA GAAGAGCAAC CTAACAAAGA ACTTCCAAAG 
CAAAATAAGG AGGGACTGGC TTCTGGTTTC GGAAAACTTA AACCCCCAGT GACACTGGAA 
AGTAAAGCAA ATTCAAAGAT GTCCTTTTCT GAATCAATGC CAAAACTAAC ACCTGCCTTC 
CCTGGGAAAG TTGTAAGTGC ACGACCTGCA GAGACTCATG AAAATCTGTT TCTTCAGCCC 
CCAAAACTTT CAAGGGAAGA ACCTTCAAAT CCCTTCTTGG CTTTTTCAGA GAAAAAAGAC 
ATTTCACCAT TTGGCTTTTC TTCTCAAACA CCTCAAAGTT CCTTTTCTAG TGCTCTCCCT 
TCTGCTTGGA CAGAACCACG CAAGTTAGAT GACTCAGGGG CTTTGAAGAA ATCTTTGTTT 
CTTTCTGTGG AATCTCCAAA AAGATTGCCT GTTTCTCCTG GTTCATCAAT CAGTGAAACT 
GTACAAAGTT CTGCCTTTCT TGGAAATGGG CGTTCCAGCT CACCAGCAAA CAGCTTAAGT 
CAGCCTATTG AAATGCCTAC ATTGTCATCT AGCCCTACTG AGGATAAGCC TGCAATTGGA 
TCTGGACAGA TAGACAATCC GTTATTAAGA ACCTTTACAG TACTCTCAAG TAGGCTCCAA 
CCTGGTGTCC ACTGCTCCTC AGATTACCCT CAAGAAAACA AAGCAGCTTT TGAATCTGTT 
AACAGATTTA CCCTAGATGA ACGAAGTAGG ACATCAAAAC AAGATTCTGA CTCCAGCACC 
AACAGTGATT TAAGTGATCT AAGTGATTCT GAGGAGCAGC TGAATTCAAA GATTGGCTAT 
AGAGGAATAC CGGAACATCT GGTTGGAAGA TTAGGGCATA ATGGAGAACG TAACGTTGAT 
TTGTTAATGG CAAAAGGTAG AGGAAAACAG CCTTCAAAAG GCAGACCAAG AACAGCTCCG 
CTAAAGGTTG GTCAATCCGT TTTGAAAGAC ATGAGCAAAG TAAGAAAGTT AAAACAGTCT 
GGAGAGCCTT TTCTTCAAGA TGGGTCTTGT ATTAATGTTG CGCCACATTT GCACAAGTGC 
CGTGAATGCA GGCTAGAAAG ATACCGCAAG TTCAAAGAGC AGGATCAGGA CGATTCAACT 
GTAGCTTGTC GATTCTTTCA TTTTCGCAGG CTGGCTTTTA CCAGGAAAGG TATTCTTCGT 
GTTGAGGGCT TCTTAAGTCC CCAGCAAAGT GATGCTGAAG CAATGCGCCT CTGGGTTCCT 
ATTTCTGTTC CAGCGGACGG CATTGATTTG GAGACCTCAA AATATATTCT AGCTAATGTT 
GGAGATCAGT TTTGCCAGTT GGTCATGTCT GAAAAAGAAG CCATGATGAT GGTGGAACCC 
CAGCAAAAGG TAGCATGGAA GAGAGCTGTA CGTGGTGTCA GGGAGATGTG TGATGTATGT 
GAGACGACAC TCTTCAACAT TCACTGGGTT TGTCGCAAGT GTGGCTTTGG CGTATGTCTT 
GATTGTTATC GGCTCCGAAA AAATCGACCT CGCAGAGATG AAATTGGTGA TGAAGAAGTC 
TTTGCGTGGC TAAAATGTGC GAAAGGACAA CCTCACGAAC CAGAGAATCT TATGCCTACC 
CAGATTATTC CAGGCACTGC TCTTTACAAC ATTGGGGATA TGGTCCATGC AGCACGAGGG 
AAATGGGGCA TTAAAGCCAA TTGTCCATGT ATTAACAGAC AGAATAAATC TGTATTAAGG 
CCAGCAGTCA CAAATGGAAT GTCTCAGCTG CCTAATGCTA ATAATGCTGC TGCTGATACA 
ACTACTACCG CAGATGAGGG GATTGAAAAT AAATCAGACC AGCCTTTAAA GAAAGAGGAA 
ACAGAAAATG AGGCATCGTC CAATAATGAT ATCGATATTG CTGCAAATAG CAGCACGGAT 
AGCAAAAACA GCCGTCCTCA ATGCCCGGAA TCTACTCCTT CCTCTGCCTT ACATTGGCTA 
GCAGACCTTG CAGCCCAGAA AGCAAAAGAA GAGACAAAAG ACAATGGAAC ACCACAATCA 
ACACCCAGCA AAGAGTCAAG TCCACCTTCT GGACTGGACC CATTAAATGC AAATGTTAAG 
ACTTCATCTC CAAAACTTTT TAATAGCCTG TTATTGGGAC CAGCTGCTTC AAATACTAAG 
ACAGAAGGCT CCAGTCTTAG AGATCTGCTC CATTCTGGCC AAGGAAAACT TTCTCAAGGA 
AATATGGAAG CAGGAATTCC ATTCCCACCT GTCTTTTCCA CAAGTTCAGC AAAGGGGAAA 
ACAAGTTTAC CAAATTTCCT TGATCACATT ATTGCTTCTG TGGTCGAAAA TAAAAAGACT 
TCAGATGCCG CTAAAAGAGC CAGTAGCTCA ACGGAAGCTC TGAGAGAGGG AAAGGAAATG 
GTCATGGGAT TAAATGTCTT TGATCCACAC ACTTCACATT CTTGGCTCTG TGATGGAAGG 
CTGCTGTGCC TTCATGATCC AAGCAACAAG AATAATTGGA AGATATTCAG GGAGTGTTGG 
AAACAGGGTC AGCCTGTACT TGTTTCTGGA GTACAGAAAA AGCTAAAGTC TGAACTTTGG 
AAACCTGAAG CTTTTAGCGT TGAGTTTGGA GACCAAGATG TGGATTTGGT GAACTGTAGA 
AATTGTGCTA TCATTTCTGA TGTGAAAGTT CGTGATTTTT GGGATGGCTT TGCAGTCATT 
GAAAAGAGGT TAAGAGCAGA AGATGGAAGT CCAATGGTCC TCAAGCTAAA AGACTGGCCA 
CCTGGAGAAG ACTTTCGGGA TATGATGCCC ACTCGATTTC AAGACTTGAT GGATAATCTT 
CCACTACCAG AATACACAAA GAGAGACGGC AGGCTCAATT TGGCTTCAAG ATTGCCTAGT 
TATTTTGTAC GACCAGATTT AGGGCCTAAA ATGTACAATG CTTATGGTTT AATTACTGCT 
GAAGACCGGC GCGTAGGTAC TACTAACCTA CATTTGGATG TGTCTGATGC AGTTAATGTT 
ATGGTATATG TGGGGATTCC AGTAGGGGAA AGCAGTCAAA ATGAAGAGGT TTTAAGAACG 
ATTGAGGAGG GTGATGCTGA TGAAGTAACA AAAAAACGTA TTCATGATGG AAAGGAGAAG 
CCTGGTGCTT TGTGGCATAT TTATGCTGCA AAGGACGCAG AGAAGATTAG AGAGCTACTC 
CGCAAGGTAG GTGATGAGCA AGGACAAGAA AACCCTCCAG ACCATGATCC AATCCATGAC 
CAAAGCTGGT ATTTAGACCA GGTACTGCGT AAGCGGCTGT ATGATGAATA TGGTGTACAA 
GGCTGGGCTA TTGTACAGTT CTTGGGAGAT GCTGTGTTCA TTCCAGCTGG TGCACCCCAT 
CAGGTGCACA ATTTGTACAG TTGTATAAAG GTAGCAGAGG ACTTTGTTTC TCCAGAACAT 
GTAAAGCATT GCTTCCGCCT GACGCAGGAA TTCAGGCATT TGTCAAACAC TCACACGAAC 
CATGAGGATA AATTACAGGT GAAGAATATT ATTTACCATG CTGTAAAGGA TGCTGTTGGA 
ACACTAAAGG CTTTTGAGTG TAGATTGGTC AGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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kdm3b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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