KDM3B gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010807344.3	XP_010805646.1	lysine-specific demethylase 3B isoform X3
XM_010807343.2	XP_010805645.1	lysine-specific demethylase 3B isoform X1
XM_005209425.3	XP_005209482.1	lysine-specific demethylase 3B isoform X4
XM_003586301.4	XP_003586349.1	lysine-specific demethylase 3B isoform X2
XM_010807343.3	XP_010805645.1	lysine-specific demethylase 3B isoform X1
XM_005209425.4	XP_005209482.1	lysine-specific demethylase 3B isoform X4
XM_010807344.2	XP_010805646.1	lysine-specific demethylase 3B isoform X3
XM_010798452.2	XP_010796754.1	lysine-specific demethylase 3B isoform X1
XM_003582422.4	XP_003582470.1	lysine-specific demethylase 3B isoform X2
XM_005199056.3	XP_005199113.1	lysine-specific demethylase 3B isoform X4
XM_003586301.5	XP_003586349.1	lysine-specific demethylase 3B isoform X2
XM_010798453.2	XP_010796755.1	lysine-specific demethylase 3B isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa05491	XM_003586301.5 Latest version!	Bos taurus lysine demethylase 3B (KDM3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa05491	XM_003582422.4 Latest version!	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa05492	XM_005209425.4 Latest version!	Bos taurus lysine demethylase 3B (KDM3B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa05492	XM_005199056.3 Latest version!	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa60541	XM_010807343.3 Latest version!	Bos taurus lysine demethylase 3B (KDM3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa60541	XM_010798452.2 Latest version!	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa60542	XM_010798453.2 Latest version!	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa60542	XM_010807344.3 Latest version!	Bos taurus lysine demethylase 3B (KDM3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OBa60541	XM_010807343.2	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa05492	XM_005209425.3	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa60542	XM_010807344.2	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa05491	XM_003586301.4	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

KDM3B ( XM_003586301.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586349.1 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa05491
Clone ID Related Accession (Same CDS sequence)	XM_003586301.4 , XM_003582422.4 , XM_003586301.5
Accession Version	XM_003586301.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104034.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586301.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586349.1
CDS	20..5299
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSEGGTLKAVKSSKGKKKRESIEGKDGRRRKSASDSGCDPASKKLKGDRGEVDSNGSDGGEASRGPWKGGNASGEPGLDQRTKQPPTTFVPQINRSIRFATYTKENGRTLVVQDEPVGGDTPLPFTPYSAATGQTPLAPEVGGAENKEAGKTLEQVGQCMVASAAVVTTASSTPTTVRISDTGLATGTGPEKQKGSWSQAPGENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_003586301.4
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586301.4

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_003582422.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003582470.1 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X2, mRNA.

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CloneID	OBa05491
Clone ID Related Accession (Same CDS sequence)	XM_003586301.4 , XM_003582422.4 , XM_003586301.5
Accession Version	XM_003582422.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582422.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003582470.1
CDS	20..5299
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSEGGTLKAVKSSKGKKKRESIEGKDGRRRKSASDSGCDPASKKLKGDRGEVDSNGSDGGEASRGPWKGGNASGEPGLDQRTKQPPTTFVPQINRSIRFATYTKENGRTLVVQDEPVGGDTPLPFTPYSAATGQTPLAPEVGGAENKEAGKTLEQVGQCMVASAAVVTTASSTPTTVRISDTGLATGTGPEKQKGSWSQAPGENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_003582422.4
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003582422.4

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_003586301.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586349.1 Bos taurus lysine demethylase 3B (KDM3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa05491
Clone ID Related Accession (Same CDS sequence)	XM_003586301.4 , XM_003582422.4 , XM_003586301.5
Accession Version	XM_003586301.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586301.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586349.1
CDS	57..5336
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSEGGTLKAVKSSKGKKKRESIEGKDGRRRKSASDSGCDPASKKLKGDRGEVDSNGSDGGEASRGPWKGGNASGEPGLDQRTKQPPTTFVPQINRSIRFATYTKENGRTLVVQDEPVGGDTPLPFTPYSAATGQTPLAPEVGGAENKEAGKTLEQVGQCMVASAAVVTTASSTPTTVRISDTGLATGTGPEKQKGSWSQAPGENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_003586301.5
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 3B (KDM3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586301.5

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_005209425.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209482.1 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X4, mRNA.

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CloneID	OBa05492
Clone ID Related Accession (Same CDS sequence)	XM_005209425.3 , XM_005209425.4 , XM_005199056.3
Accession Version	XM_005209425.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104034.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209425.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209482.1
CDS	18..4697
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_005209425.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209425.3

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_005209425.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209482.1 Bos taurus lysine demethylase 3B (KDM3B), transcript variant X4, mRNA.

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CloneID	OBa05492
Clone ID Related Accession (Same CDS sequence)	XM_005209425.3 , XM_005209425.4 , XM_005199056.3
Accession Version	XM_005209425.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209425.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209482.1
CDS	16..4695
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_005209425.4
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 3B (KDM3B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209425.4

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_005199056.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005199113.1 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X4, mRNA.

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CloneID	OBa05492
Clone ID Related Accession (Same CDS sequence)	XM_005209425.3 , XM_005209425.4 , XM_005199056.3
Accession Version	XM_005199056.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005199113.1
CDS	18..4697
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_005199056.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005199056.3

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT 
GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA 
GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA 
AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG 
ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA 
AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC 
AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT 
AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG 
CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC 
CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC 
ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT 
GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA 
AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC 
TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA 
GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC 
CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT 
TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG 
AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA 
AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT 
GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC 
TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG 
CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT 
GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT 
CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT 
GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT 
GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT 
GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG 
ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA 
GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT 
GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC 
TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC 
ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA 
GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT 
CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC 
GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_010807343.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805645.1 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X1, mRNA.

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CloneID	OBa60541
Clone ID Related Accession (Same CDS sequence)	XM_010807343.2 , XM_010807343.3 , XM_010798452.2
Accession Version	XM_010807343.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104034.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805645.1
CDS	20..5329
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSEGGTLKAVKSSKGKKKRESIEGKDGRRRKSASDSGCDPASKKLKGDRGEVDSNGSDGGEASRGPWKGGNASGEPGLDQRTKQPPTTFVPQINRSIRFATYTKENGRTLVVQDEPVGGDTPLPFTPYSAATGQTPLAPEVGGAENKEAGKTLEQVGQCMVASAAVVTTASSTPTTVRISDTGLATGTGPEKQKGSWSQAPGENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSGEQMLSYTAEETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_010807343.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807343.2

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_010807343.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805645.1 Bos taurus lysine demethylase 3B (KDM3B), transcript variant X1, mRNA.

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CloneID	OBa60541
Clone ID Related Accession (Same CDS sequence)	XM_010807343.2 , XM_010807343.3 , XM_010798452.2
Accession Version	XM_010807343.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807343.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805645.1
CDS	16..5325
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSEGGTLKAVKSSKGKKKRESIEGKDGRRRKSASDSGCDPASKKLKGDRGEVDSNGSDGGEASRGPWKGGNASGEPGLDQRTKQPPTTFVPQINRSIRFATYTKENGRTLVVQDEPVGGDTPLPFTPYSAATGQTPLAPEVGGAENKEAGKTLEQVGQCMVASAAVVTTASSTPTTVRISDTGLATGTGPEKQKGSWSQAPGENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSGEQMLSYTAEETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_010807343.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 3B (KDM3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807343.3

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_010798452.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010796754.1 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X1, mRNA.

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CloneID	OBa60541
Clone ID Related Accession (Same CDS sequence)	XM_010807343.2 , XM_010807343.3 , XM_010798452.2
Accession Version	XM_010798452.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010796754.1
CDS	20..5329
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSEGGTLKAVKSSKGKKKRESIEGKDGRRRKSASDSGCDPASKKLKGDRGEVDSNGSDGGEASRGPWKGGNASGEPGLDQRTKQPPTTFVPQINRSIRFATYTKENGRTLVVQDEPVGGDTPLPFTPYSAATGQTPLAPEVGGAENKEAGKTLEQVGQCMVASAAVVTTASSTPTTVRISDTGLATGTGPEKQKGSWSQAPGENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSGEQMLSYTAEETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_010798452.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010798452.2

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC 
ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA 
TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG 
GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA 
ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT 
TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA 
CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG 
GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG 
GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC 
ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA 
GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_010807344.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805646.1 Bos taurus lysine demethylase 3B (KDM3B), transcript variant X3, mRNA.

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CloneID	OBa60542
Clone ID Related Accession (Same CDS sequence)	XM_010807344.3 , XM_010807344.2 , XM_010798453.2
Accession Version	XM_010807344.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807344.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805646.1
CDS	16..4725
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSGEQMLSYTAEETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_010807344.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 3B (KDM3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807344.3

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_010807344.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805646.1 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X3, mRNA.

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CloneID	OBa60542
Clone ID Related Accession (Same CDS sequence)	XM_010807344.3 , XM_010807344.2 , XM_010798453.2
Accession Version	XM_010807344.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104034.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807344.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805646.1
CDS	19..4728
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSGEQMLSYTAEETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_010807344.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807344.2

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_010798453.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010796755.1 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X3, mRNA.

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CloneID	OBa60542
Clone ID Related Accession (Same CDS sequence)	XM_010807344.3 , XM_010807344.2 , XM_010798453.2
Accession Version	XM_010798453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 3B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010796755.1
CDS	19..4728
Translation	MADAAASPVGKRLLLLFADTAGSSSASVPAAAAAAAGGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPVLIQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDGQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVQGHRVKVYQPEGEESWLCGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLVDNSAPQSENSRNSVLASSGFGASLPGSSQALTFGSGRSQSNGVLATENKPLGFSFGCSPAPESQKDSDLSKNLFFQCMSQTLPTSNYFTTVSENLADDSSSRDSFKQSLESLCKGRSTLGADTKSGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPDSHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKAAPSWPESHSSTDSASLAKKKTLFITTDSSKLVSGVLGSALTTGGPSLSAMGNGRSSSPTSGLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGSFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKGKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSGEQMLSYTAEETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSSGGGTAPGAQPETDHVPKADSTDSRSDEPLKADSSTSNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKNKASLPNFLDHIIASVVENKKTSDAAKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_010798453.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010798453.2

ORF Insert Sequence:

ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG 
GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG 
GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC 
TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG 
GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA 
GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA 
TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG 
ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT 
CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT 
GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG 
ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG 
AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT 
TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA 
GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC 
TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC 
TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA 
CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC 
TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG 
CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT 
TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG 
AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT 
GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT 
TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG 
TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT 
GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC 
TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT 
AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT 
TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA 
AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC 
GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG 
GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG 
AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG 
AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG 
TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT 
CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC 
CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG 
CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA 
GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG 
ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA 
GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC 
TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC 
CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT 
GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT 
CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT 
GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA 
TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT 
GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA 
CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG 
AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC 
CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG 
GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT 
TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG 
CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC 
CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC 
ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA 
CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT 
GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG 
TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT 
CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC 
CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA 
GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT 
ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA 
TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA 
GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA 
GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA 
AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA 
AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT 
GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG 
GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT 
TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT 
GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG 
TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC 
ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC 
AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC 
TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT 
AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG 
GCTCATGAGT CCAAGCTGGC AAGATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KDM3B
Entrez Gene ID	100848816
Full Name	lysine demethylase 3B
General protein information	Preferred Names lysine demethylase 3B Names lysine-specific demethylase 3B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	KDM3B	XP_517956.3
Mus musculus (house mouse)	Kdm3b	NP_001074725.1
Danio rerio (zebrafish)	wu:fe01c10	XP_005173195.1
	KDM3B	XP_002804573.1
Gallus gallus (chicken)	KDM3B	XP_004945121.1
Xenopus tropicalis (tropical clawed frog)	kdm3b	XP_002936894.1
Bos taurus (cattle)	KDM3B	XP_003586349.1
Homo sapiens (human)	KDM3B	NP_057688.2
Canis lupus familiaris (dog)	KDM3B	XP_531921.3
Rattus norvegicus (Norway rat)	Kdm3b	XP_006254677.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KDM3B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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