kdm3b gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021472769.1	XP_021328444.1	lysine-specific demethylase 3B isoform X6
XM_005173140.3	XP_005173197.1	lysine-specific demethylase 3B isoform X3
XM_005173142.3	XP_005173199.1	lysine-specific demethylase 3B isoform X5
XM_021472770.1	XP_021328445.1	lysine-specific demethylase 3B isoform X7
XM_005173140.4	XP_005173197.1	lysine-specific demethylase 3B isoform X3
XM_005173138.3	XP_005173195.1	lysine-specific demethylase 3B isoform X1
XM_005173139.3	XP_005173196.1	lysine-specific demethylase 3B isoform X2
XM_005173141.3	XP_005173198.1	lysine-specific demethylase 3B isoform X4
XM_021472771.1	XP_021328446.1	lysine-specific demethylase 3B isoform X8
XM_005173142.4	XP_005173199.1	lysine-specific demethylase 3B isoform X5
XM_005173138.4	XP_005173195.1	lysine-specific demethylase 3B isoform X1
XM_005173139.4	XP_005173196.1	lysine-specific demethylase 3B isoform X2
XM_005173141.4	XP_005173198.1	lysine-specific demethylase 3B isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145598	XM_021472769.1 Latest version!	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa145599	XM_021472770.1 Latest version!	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa145600	XM_021472771.1 Latest version!	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa19557	XM_005173138.4 Latest version!	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa19558	XM_005173139.4 Latest version!	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa19559	XM_005173140.4 Latest version!	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa19560	XM_005173141.4 Latest version!	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa19561	XM_005173142.4 Latest version!	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
View Old Accession Versions >>
ODa19559	XM_005173140.3	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa19561	XM_005173142.3	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa19557	XM_005173138.3	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa19558	XM_005173139.3	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa19560	XM_005173141.3	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
Hide Old Accession Versions >>

kdm3b ( XM_021472769.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328444.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa145598
Clone ID Related Accession (Same CDS sequence)	XM_021472769.1
Accession Version	XM_021472769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 3B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCACT GTTTTAAATG TGAAAAACTT GCATCTTGTA TTTTCTTAAT TGGTCCTGTG 
TTTTATTTGG TGCAGTCGGG TGCAGCTCAC AGTTCGGGGA ATGGAACCAC GTCTACTTCA 
TCCACAACCC GTCCAAATGA AGAATCTGCA GCTGGTGTTG TAGTCAAAAC TGAACCCGTT 
GAAGAGCCTA CAAGTGCTGA TACCACATCA AGTACCAGTG GGACAAATAG CAGCACATCT 
AGCCCTGCTC CTGCTCCAAC TTCTGCTCCT ACTCTTCTTC CTCCTTCTCT TCCTCCTCCT 
TCACTTCCTC CTCCTTCACT TCCTCCTGCT CCTGCACTTG TACCTACACC TCCTGCAAAG 
GATAGCAAGA GCAGTTCTTC AGCACTTCAC TGGCTGGCAG ATCTGGCCAC ACAAAAGGAG 
CCCAAAGAGT CTCTGCGCTC CATGATGGGT CGTGACTCTC GTTCTCCATT TGGCCTGGAC 
TCGTTCAGCA CACTTTCCAA ACCATCAGGG TCCAGTCCTA AGCTGTTCAA CAGTCTGCTG 
CTGGGTGCAG GCCCTTCACA ACCCAAAGCA GAGGGAACCA GCCTCCGAGA TCTGCTGAAC 
TCTGGCTCTG GCAAACTCCC GCAAGGGCCC ACAGAGGGAG GTGTCCCATT TCCTCCAGTG 
TTCTCCACTG CTAGTCAGGC TGATAAAATG AAGGGAGGTC TTCCTAACTT CCTGGATCAT 
ATCATTGCAT CAGTAGTGGA GACTAAGAAG TCAGAGGTTC GTCGTGCATC AGGTTCAGCA 
GAAGTCATCG AGTCTGCCAC TGCTCCTCGC AGAGAGGGGG TGATGGGGCT GAGTGTCCTG 
GATCCACACA CTTCACACTC TTGGCTTTGT GACGGCCGTT TGCTCTGCCT GCAGGACCCC 
AGTAACCCCA ACAACTGGAA GATCTTCAGA GAGTGCTGGA AACAAGGACA GCCTGTGCTG 
GTCTCAGGCA TACACAGGAA GCTGAAGGAA CACTTATGGA GGCCTGAGGC CTTCAGCGAG 
GAGTTTGGAG ACCAGGACGT GGACCTGGTC AACTGTAGGA ACTGCGCCAT CATCTCTGAT 
GTTAAAGTCA GGGAATTTTG GGATGGCTTC CAGGTCATTT CCAAGCGATT GCAAGGTAGT 
GATGGCCAGC CTATGGTACT AAAACTTAAA GACTGGCCTC CTGGAGAAGA TTTTCGAGAT 
ATGATGCCCA CTCGGTTTAA TGATCTCATG GACAATCTTC CTTTGCCCGA GTACACAAAG 
AGAGATGGCC GTCTCAACCT GGCCTCTCGT CTTCCAAACT TCTTTGTTCG GCCAGATCTG 
GGCCCTAAAA TGTACAATGC GTATGGTCTG ATCTCCACAG AAGACAGGAA GGTCGGCACC 
ACTAACTTGC ATTTGGATGT GTCTGATGCC GTCAACGTTA TGGTGTATGT GGGCATCCCA 
GAAGGAGAGA ATGACCAAGA AAGTGAAGCA GACCTCGCTG GATTCAAAGA GGTGATGCAG 
ACTATTGAAG AGGGTGATGT AGATGACATG ACGAAAAGAA GAGTCTACGA GATCAAGGAG 
AAACCTGGGG CTCTCTGGCA CATCTATGCT GCCAAAGACG CTGAGAAGAT CCGAGAGCTC 
TTACGAAAGG TTGGAGAGGA ACAAGGTCAA GAGAACCCAC CAGACCATGA TCCTATCCAT 
GACCAGAGCT GGTACCTGGA CCAGACGTTG CGTCGCAGGC TGTATGAGGA GTATGGAGTC 
CAGGGATGGT CCATTGTGCA GTTTTTGGGA GATGCAGTGT TCATCCCAGC TGGAGCACCA 
CATCAGGTGC ACAACCTGTA CAGCTGTATC AAAGCTGCAG AAGATTTTGT TTCTCCAGAG 
CATGTGAAGC ACTGTTTTAG ACTGACGCAA GAGTTTCGCC ACCTTTCCAA CACTCACACA 
AACCACGAGG ACAAGCTACA GGTGAAGAAC ATCATCTACC ATGCTGTGAA GGATGCTGTG 
GCAACACTAA AAGCCCATGA GCCCAAATTA GGCCGTTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328444.1
CDS	16..2037
Translation	MFHCFKCEKLASCIFLIGPVFYLVQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASQADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEADLAGFKEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_021472769.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472769.1

ORF Insert Sequence:

ATGTTTCACT GTTTTAAATG TGAAAAACTT GCATCTTGTA TTTTCTTAAT TGGTCCTGTG 
TTTTATTTGG TGCAGTCGGG TGCAGCTCAC AGTTCGGGGA ATGGAACCAC GTCTACTTCA 
TCCACAACCC GTCCAAATGA AGAATCTGCA GCTGGTGTTG TAGTCAAAAC TGAACCCGTT 
GAAGAGCCTA CAAGTGCTGA TACCACATCA AGTACCAGTG GGACAAATAG CAGCACATCT 
AGCCCTGCTC CTGCTCCAAC TTCTGCTCCT ACTCTTCTTC CTCCTTCTCT TCCTCCTCCT 
TCACTTCCTC CTCCTTCACT TCCTCCTGCT CCTGCACTTG TACCTACACC TCCTGCAAAG 
GATAGCAAGA GCAGTTCTTC AGCACTTCAC TGGCTGGCAG ATCTGGCCAC ACAAAAGGAG 
CCCAAAGAGT CTCTGCGCTC CATGATGGGT CGTGACTCTC GTTCTCCATT TGGCCTGGAC 
TCGTTCAGCA CACTTTCCAA ACCATCAGGG TCCAGTCCTA AGCTGTTCAA CAGTCTGCTG 
CTGGGTGCAG GCCCTTCACA ACCCAAAGCA GAGGGAACCA GCCTCCGAGA TCTGCTGAAC 
TCTGGCTCTG GCAAACTCCC GCAAGGGCCC ACAGAGGGAG GTGTCCCATT TCCTCCAGTG 
TTCTCCACTG CTAGTCAGGC TGATAAAATG AAGGGAGGTC TTCCTAACTT CCTGGATCAT 
ATCATTGCAT CAGTAGTGGA GACTAAGAAG TCAGAGGTTC GTCGTGCATC AGGTTCAGCA 
GAAGTCATCG AGTCTGCCAC TGCTCCTCGC AGAGAGGGGG TGATGGGGCT GAGTGTCCTG 
GATCCACACA CTTCACACTC TTGGCTTTGT GACGGCCGTT TGCTCTGCCT GCAGGACCCC 
AGTAACCCCA ACAACTGGAA GATCTTCAGA GAGTGCTGGA AACAAGGACA GCCTGTGCTG 
GTCTCAGGCA TACACAGGAA GCTGAAGGAA CACTTATGGA GGCCTGAGGC CTTCAGCGAG 
GAGTTTGGAG ACCAGGACGT GGACCTGGTC AACTGTAGGA ACTGCGCCAT CATCTCTGAT 
GTTAAAGTCA GGGAATTTTG GGATGGCTTC CAGGTCATTT CCAAGCGATT GCAAGGTAGT 
GATGGCCAGC CTATGGTACT AAAACTTAAA GACTGGCCTC CTGGAGAAGA TTTTCGAGAT 
ATGATGCCCA CTCGGTTTAA TGATCTCATG GACAATCTTC CTTTGCCCGA GTACACAAAG 
AGAGATGGCC GTCTCAACCT GGCCTCTCGT CTTCCAAACT TCTTTGTTCG GCCAGATCTG 
GGCCCTAAAA TGTACAATGC GTATGGTCTG ATCTCCACAG AAGACAGGAA GGTCGGCACC 
ACTAACTTGC ATTTGGATGT GTCTGATGCC GTCAACGTTA TGGTGTATGT GGGCATCCCA 
GAAGGAGAGA ATGACCAAGA AAGTGAAGCA GACCTCGCTG GATTCAAAGA GGTGATGCAG 
ACTATTGAAG AGGGTGATGT AGATGACATG ACGAAAAGAA GAGTCTACGA GATCAAGGAG 
AAACCTGGGG CTCTCTGGCA CATCTATGCT GCCAAAGACG CTGAGAAGAT CCGAGAGCTC 
TTACGAAAGG TTGGAGAGGA ACAAGGTCAA GAGAACCCAC CAGACCATGA TCCTATCCAT 
GACCAGAGCT GGTACCTGGA CCAGACGTTG CGTCGCAGGC TGTATGAGGA GTATGGAGTC 
CAGGGATGGT CCATTGTGCA GTTTTTGGGA GATGCAGTGT TCATCCCAGC TGGAGCACCA 
CATCAGGTGC ACAACCTGTA CAGCTGTATC AAAGCTGCAG AAGATTTTGT TTCTCCAGAG 
CATGTGAAGC ACTGTTTTAG ACTGACGCAA GAGTTTCGCC ACCTTTCCAA CACTCACACA 
AACCACGAGG ACAAGCTACA GGTGAAGAAC ATCATCTACC ATGCTGTGAA GGATGCTGTG 
GCAACACTAA AAGCCCATGA GCCCAAATTA GGCCGTTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_021472770.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328445.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa145599
Clone ID Related Accession (Same CDS sequence)	XM_021472770.1
Accession Version	XM_021472770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 3B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCACT GTTTTAAATG TGAAAAACTT GCATCTTGTA TTTTCTTAAT TGGTCCTGTG 
TTTTATTTGG TGCAGTCGGG TGCAGCTCAC AGTTCGGGGA ATGGAACCAC GTCTACTTCA 
TCCACAACCC GTCCAAATGA AGAATCTGCA GCTGGTGTTG TAGTCAAAAC TGAACCCGTT 
GAAGAGCCTA CAAGTGCTGA TACCACATCA AGTACCAGTG GGACAAATAG CAGCACATCT 
AGCCCTGCTC CTGCTCCAAC TTCTGCTCCT ACTCTTCTTC CTCCTTCTCT TCCTCCTCCT 
TCACTTCCTC CTCCTTCACT TCCTCCTGCT CCTGCACTTG TACCTACACC TCCTGCAAAG 
GATAGCAAGA GCAGTTCTTC AGCACTTCAC TGGCTGGCAG ATCTGGCCAC ACAAAAGGAG 
CCCAAAGAGT CTCTGCGCTC CATGATGGGT CGTGACTCTC GTTCTCCATT TGGCCTGGAC 
TCGTTCAGCA CACTTTCCAA ACCATCAGGG TCCAGTCCTA AGCTGTTCAA CAGTCTGCTG 
CTGGGTGCAG GCCCTTCACA ACCCAAAGCA GAGGGAACCA GCCTCCGAGA TCTGCTGAAC 
TCTGGCTCTG GCAAACTCCC GCAAGGGCCC ACAGAGGGAG GTGTCCCATT TCCTCCAGTG 
TTCTCCACTG CTAGTGCTGA TAAAATGAAG GGAGGTCTTC CTAACTTCCT GGATCATATC 
ATTGCATCAG TAGTGGAGAC TAAGAAGTCA GAGGTTCGTC GTGCATCAGG TTCAGCAGAA 
GTCATCGAGT CTGCCACTGC TCCTCGCAGA GAGGGGGTGA TGGGGCTGAG TGTCCTGGAT 
CCACACACTT CACACTCTTG GCTTTGTGAC GGCCGTTTGC TCTGCCTGCA GGACCCCAGT 
AACCCCAACA ACTGGAAGAT CTTCAGAGAG TGCTGGAAAC AAGGACAGCC TGTGCTGGTC 
TCAGGCATAC ACAGGAAGCT GAAGGAACAC TTATGGAGGC CTGAGGCCTT CAGCGAGGAG 
TTTGGAGACC AGGACGTGGA CCTGGTCAAC TGTAGGAACT GCGCCATCAT CTCTGATGTT 
AAAGTCAGGG AATTTTGGGA TGGCTTCCAG GTCATTTCCA AGCGATTGCA AGGTAGTGAT 
GGCCAGCCTA TGGTACTAAA ACTTAAAGAC TGGCCTCCTG GAGAAGATTT TCGAGATATG 
ATGCCCACTC GGTTTAATGA TCTCATGGAC AATCTTCCTT TGCCCGAGTA CACAAAGAGA 
GATGGCCGTC TCAACCTGGC CTCTCGTCTT CCAAACTTCT TTGTTCGGCC AGATCTGGGC 
CCTAAAATGT ACAATGCGTA TGGTCTGATC TCCACAGAAG ACAGGAAGGT CGGCACCACT 
AACTTGCATT TGGATGTGTC TGATGCCGTC AACGTTATGG TGTATGTGGG CATCCCAGAA 
GGAGAGAATG ACCAAGAAAG TGAAGCAGAC CTCGCTGGAT TCAAAGAGGT GATGCAGACT 
ATTGAAGAGG GTGATGTAGA TGACATGACG AAAAGAAGAG TCTACGAGAT CAAGGAGAAA 
CCTGGGGCTC TCTGGCACAT CTATGCTGCC AAAGACGCTG AGAAGATCCG AGAGCTCTTA 
CGAAAGGTTG GAGAGGAACA AGGTCAAGAG AACCCACCAG ACCATGATCC TATCCATGAC 
CAGAGCTGGT ACCTGGACCA GACGTTGCGT CGCAGGCTGT ATGAGGAGTA TGGAGTCCAG 
GGATGGTCCA TTGTGCAGTT TTTGGGAGAT GCAGTGTTCA TCCCAGCTGG AGCACCACAT 
CAGGTGCACA ACCTGTACAG CTGTATCAAA GCTGCAGAAG ATTTTGTTTC TCCAGAGCAT 
GTGAAGCACT GTTTTAGACT GACGCAAGAG TTTCGCCACC TTTCCAACAC TCACACAAAC 
CACGAGGACA AGCTACAGGT GAAGAACATC ATCTACCATG CTGTGAAGGA TGCTGTGGCA 
ACACTAAAAG CCCATGAGCC CAAATTAGGC CGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328445.1
CDS	16..2034
Translation	MFHCFKCEKLASCIFLIGPVFYLVQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEADLAGFKEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_021472770.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472770.1

ORF Insert Sequence:

ATGTTTCACT GTTTTAAATG TGAAAAACTT GCATCTTGTA TTTTCTTAAT TGGTCCTGTG 
TTTTATTTGG TGCAGTCGGG TGCAGCTCAC AGTTCGGGGA ATGGAACCAC GTCTACTTCA 
TCCACAACCC GTCCAAATGA AGAATCTGCA GCTGGTGTTG TAGTCAAAAC TGAACCCGTT 
GAAGAGCCTA CAAGTGCTGA TACCACATCA AGTACCAGTG GGACAAATAG CAGCACATCT 
AGCCCTGCTC CTGCTCCAAC TTCTGCTCCT ACTCTTCTTC CTCCTTCTCT TCCTCCTCCT 
TCACTTCCTC CTCCTTCACT TCCTCCTGCT CCTGCACTTG TACCTACACC TCCTGCAAAG 
GATAGCAAGA GCAGTTCTTC AGCACTTCAC TGGCTGGCAG ATCTGGCCAC ACAAAAGGAG 
CCCAAAGAGT CTCTGCGCTC CATGATGGGT CGTGACTCTC GTTCTCCATT TGGCCTGGAC 
TCGTTCAGCA CACTTTCCAA ACCATCAGGG TCCAGTCCTA AGCTGTTCAA CAGTCTGCTG 
CTGGGTGCAG GCCCTTCACA ACCCAAAGCA GAGGGAACCA GCCTCCGAGA TCTGCTGAAC 
TCTGGCTCTG GCAAACTCCC GCAAGGGCCC ACAGAGGGAG GTGTCCCATT TCCTCCAGTG 
TTCTCCACTG CTAGTGCTGA TAAAATGAAG GGAGGTCTTC CTAACTTCCT GGATCATATC 
ATTGCATCAG TAGTGGAGAC TAAGAAGTCA GAGGTTCGTC GTGCATCAGG TTCAGCAGAA 
GTCATCGAGT CTGCCACTGC TCCTCGCAGA GAGGGGGTGA TGGGGCTGAG TGTCCTGGAT 
CCACACACTT CACACTCTTG GCTTTGTGAC GGCCGTTTGC TCTGCCTGCA GGACCCCAGT 
AACCCCAACA ACTGGAAGAT CTTCAGAGAG TGCTGGAAAC AAGGACAGCC TGTGCTGGTC 
TCAGGCATAC ACAGGAAGCT GAAGGAACAC TTATGGAGGC CTGAGGCCTT CAGCGAGGAG 
TTTGGAGACC AGGACGTGGA CCTGGTCAAC TGTAGGAACT GCGCCATCAT CTCTGATGTT 
AAAGTCAGGG AATTTTGGGA TGGCTTCCAG GTCATTTCCA AGCGATTGCA AGGTAGTGAT 
GGCCAGCCTA TGGTACTAAA ACTTAAAGAC TGGCCTCCTG GAGAAGATTT TCGAGATATG 
ATGCCCACTC GGTTTAATGA TCTCATGGAC AATCTTCCTT TGCCCGAGTA CACAAAGAGA 
GATGGCCGTC TCAACCTGGC CTCTCGTCTT CCAAACTTCT TTGTTCGGCC AGATCTGGGC 
CCTAAAATGT ACAATGCGTA TGGTCTGATC TCCACAGAAG ACAGGAAGGT CGGCACCACT 
AACTTGCATT TGGATGTGTC TGATGCCGTC AACGTTATGG TGTATGTGGG CATCCCAGAA 
GGAGAGAATG ACCAAGAAAG TGAAGCAGAC CTCGCTGGAT TCAAAGAGGT GATGCAGACT 
ATTGAAGAGG GTGATGTAGA TGACATGACG AAAAGAAGAG TCTACGAGAT CAAGGAGAAA 
CCTGGGGCTC TCTGGCACAT CTATGCTGCC AAAGACGCTG AGAAGATCCG AGAGCTCTTA 
CGAAAGGTTG GAGAGGAACA AGGTCAAGAG AACCCACCAG ACCATGATCC TATCCATGAC 
CAGAGCTGGT ACCTGGACCA GACGTTGCGT CGCAGGCTGT ATGAGGAGTA TGGAGTCCAG 
GGATGGTCCA TTGTGCAGTT TTTGGGAGAT GCAGTGTTCA TCCCAGCTGG AGCACCACAT 
CAGGTGCACA ACCTGTACAG CTGTATCAAA GCTGCAGAAG ATTTTGTTTC TCCAGAGCAT 
GTGAAGCACT GTTTTAGACT GACGCAAGAG TTTCGCCACC TTTCCAACAC TCACACAAAC 
CACGAGGACA AGCTACAGGT GAAGAACATC ATCTACCATG CTGTGAAGGA TGCTGTGGCA 
ACACTAAAAG CCCATGAGCC CAAATTAGGC CGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_021472771.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328446.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa145600
Clone ID Related Accession (Same CDS sequence)	XM_021472771.1
Accession Version	XM_021472771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 3B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCACT GTTTTAAATG TGAAAAACTT GCATCTTGTA TTTTCTTAAT TGGTCCTGTG 
TTTTATTTGG TGCAGTCGGG TGCAGCTCAC AGTTCGGGGA ATGGAACCAC GTCTACTTCA 
TCCACAACCC GTCCAAATGA AGAATCTGCA GCTGGTGTTG TAGTCAAAAC TGAACCCGTT 
GAAGAGCCTA CAAGTGCTGA TACCACATCA AGTACCAGTG GGACAAATAG CAGCACATCT 
AGCCCTGCTC CTGCTCCAAC TTCTGCTCCT ACTCTTCTTC CTCCTTCTCT TCCTCCTCCT 
TCACTTCCTC CTCCTTCACT TCCTCCTGCT CCTGCACTTG TACCTACACC TCCTGCAAAG 
GATAGCAAGA GCAGTTCTTC AGCACTTCAC TGGCTGGCAG ATCTGGCCAC ACAAAAGGAG 
CCCAAAGAGT CTCTGCGCTC CATGATGGGT CGTGACTCTC GTTCTCCATT TGGCCTGGAC 
TCGTTCAGCA CACTTTCCAA ACCATCAGGG TCCAGTCCTA AGCTGTTCAA CAGTCTGCTG 
CTGGGTGCAG GCCCTTCACA ACCCAAAGCA GAGGGAACCA GCCTCCGAGA TCTGCTGAAC 
TCTGGCTCTG GCAAACTCCC GCAAGGGCCC ACAGAGGGAG GTGTCCCATT TCCTCCAGTG 
TTCTCCACTG CTAGTCAGGC TGATAAAATG AAGGGAGGTC TTCCTAACTT CCTGGATCAT 
ATCATTGCAT CAGTAGTGGA GACTAAGAAG TCAGAGGTTC GTCGTGCATC AGGTTCAGCA 
GAAGTCATCG AGTCTGCCAC TGCTCCTCGC AGAGAGGGGG TGATGGGGCT GAGTGTCCTG 
GATCCACACA CTTCACACTC TTGGCTTTGT GACGGCCGTT TGCTCTGCCT GCAGGACCCC 
AGTAACCCCA ACAACTGGAA GATCTTCAGA GAGTGCTGGA AACAAGGACA GCCTGTGCTG 
GTCTCAGGCA TACACAGGAA GCTGAAGGAA CACTTATGGA GGCCTGAGGC CTTCAGCGAG 
GAGTTTGGAG ACCAGGACGT GGACCTGGTC AACTGTAGGA ACTGCGCCAT CATCTCTGAT 
GTTAAAGTCA GGGAATTTTG GGATGGCTTC CAGGTCATTT CCAAGCGATT GCAAGGTAGT 
GATGGCCAGC CTATGGTACT AAAACTTAAA GACTGGCCTC CTGGAGAAGA TTTTCGAGAT 
ATGATGCCCA CTCGGTTTAA TGATCTCATG GACAATCTTC CTTTGCCCGA GTACACAAAG 
AGAGATGGCC GTCTCAACCT GGCCTCTCGT CTTCCAAACT TCTTTGTTCG GCCAGATCTG 
GGCCCTAAAA TGTACAATGC GTATGGTCTG ATCTCCACAG AAGACAGGAA GGTCGGCACC 
ACTAACTTGC ATTTGGATGT GTCTGATGCC GTCAACGTTA TGGTGTATGT GGGCATCCCA 
GAAGGAGAGA ATGACCAAGA AAGTGAGGTG ATGCAGACTA TTGAAGAGGG TGATGTAGAT 
GACATGACGA AAAGAAGAGT CTACGAGATC AAGGAGAAAC CTGGGGCTCT CTGGCACATC 
TATGCTGCCA AAGACGCTGA GAAGATCCGA GAGCTCTTAC GAAAGGTTGG AGAGGAACAA 
GGTCAAGAGA ACCCACCAGA CCATGATCCT ATCCATGACC AGAGCTGGTA CCTGGACCAG 
ACGTTGCGTC GCAGGCTGTA TGAGGAGTAT GGAGTCCAGG GATGGTCCAT TGTGCAGTTT 
TTGGGAGATG CAGTGTTCAT CCCAGCTGGA GCACCACATC AGGTGCACAA CCTGTACAGC 
TGTATCAAAG CTGCAGAAGA TTTTGTTTCT CCAGAGCATG TGAAGCACTG TTTTAGACTG 
ACGCAAGAGT TTCGCCACCT TTCCAACACT CACACAAACC ACGAGGACAA GCTACAGGTG 
AAGAACATCA TCTACCATGC TGTGAAGGAT GCTGTGGCAA CACTAAAAGC CCATGAGCCC 
AAATTAGGCC GTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328446.1
CDS	16..2013
Translation	MFHCFKCEKLASCIFLIGPVFYLVQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASQADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_021472771.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472771.1

ORF Insert Sequence:

ATGTTTCACT GTTTTAAATG TGAAAAACTT GCATCTTGTA TTTTCTTAAT TGGTCCTGTG 
TTTTATTTGG TGCAGTCGGG TGCAGCTCAC AGTTCGGGGA ATGGAACCAC GTCTACTTCA 
TCCACAACCC GTCCAAATGA AGAATCTGCA GCTGGTGTTG TAGTCAAAAC TGAACCCGTT 
GAAGAGCCTA CAAGTGCTGA TACCACATCA AGTACCAGTG GGACAAATAG CAGCACATCT 
AGCCCTGCTC CTGCTCCAAC TTCTGCTCCT ACTCTTCTTC CTCCTTCTCT TCCTCCTCCT 
TCACTTCCTC CTCCTTCACT TCCTCCTGCT CCTGCACTTG TACCTACACC TCCTGCAAAG 
GATAGCAAGA GCAGTTCTTC AGCACTTCAC TGGCTGGCAG ATCTGGCCAC ACAAAAGGAG 
CCCAAAGAGT CTCTGCGCTC CATGATGGGT CGTGACTCTC GTTCTCCATT TGGCCTGGAC 
TCGTTCAGCA CACTTTCCAA ACCATCAGGG TCCAGTCCTA AGCTGTTCAA CAGTCTGCTG 
CTGGGTGCAG GCCCTTCACA ACCCAAAGCA GAGGGAACCA GCCTCCGAGA TCTGCTGAAC 
TCTGGCTCTG GCAAACTCCC GCAAGGGCCC ACAGAGGGAG GTGTCCCATT TCCTCCAGTG 
TTCTCCACTG CTAGTCAGGC TGATAAAATG AAGGGAGGTC TTCCTAACTT CCTGGATCAT 
ATCATTGCAT CAGTAGTGGA GACTAAGAAG TCAGAGGTTC GTCGTGCATC AGGTTCAGCA 
GAAGTCATCG AGTCTGCCAC TGCTCCTCGC AGAGAGGGGG TGATGGGGCT GAGTGTCCTG 
GATCCACACA CTTCACACTC TTGGCTTTGT GACGGCCGTT TGCTCTGCCT GCAGGACCCC 
AGTAACCCCA ACAACTGGAA GATCTTCAGA GAGTGCTGGA AACAAGGACA GCCTGTGCTG 
GTCTCAGGCA TACACAGGAA GCTGAAGGAA CACTTATGGA GGCCTGAGGC CTTCAGCGAG 
GAGTTTGGAG ACCAGGACGT GGACCTGGTC AACTGTAGGA ACTGCGCCAT CATCTCTGAT 
GTTAAAGTCA GGGAATTTTG GGATGGCTTC CAGGTCATTT CCAAGCGATT GCAAGGTAGT 
GATGGCCAGC CTATGGTACT AAAACTTAAA GACTGGCCTC CTGGAGAAGA TTTTCGAGAT 
ATGATGCCCA CTCGGTTTAA TGATCTCATG GACAATCTTC CTTTGCCCGA GTACACAAAG 
AGAGATGGCC GTCTCAACCT GGCCTCTCGT CTTCCAAACT TCTTTGTTCG GCCAGATCTG 
GGCCCTAAAA TGTACAATGC GTATGGTCTG ATCTCCACAG AAGACAGGAA GGTCGGCACC 
ACTAACTTGC ATTTGGATGT GTCTGATGCC GTCAACGTTA TGGTGTATGT GGGCATCCCA 
GAAGGAGAGA ATGACCAAGA AAGTGAGGTG ATGCAGACTA TTGAAGAGGG TGATGTAGAT 
GACATGACGA AAAGAAGAGT CTACGAGATC AAGGAGAAAC CTGGGGCTCT CTGGCACATC 
TATGCTGCCA AAGACGCTGA GAAGATCCGA GAGCTCTTAC GAAAGGTTGG AGAGGAACAA 
GGTCAAGAGA ACCCACCAGA CCATGATCCT ATCCATGACC AGAGCTGGTA CCTGGACCAG 
ACGTTGCGTC GCAGGCTGTA TGAGGAGTAT GGAGTCCAGG GATGGTCCAT TGTGCAGTTT 
TTGGGAGATG CAGTGTTCAT CCCAGCTGGA GCACCACATC AGGTGCACAA CCTGTACAGC 
TGTATCAAAG CTGCAGAAGA TTTTGTTTCT CCAGAGCATG TGAAGCACTG TTTTAGACTG 
ACGCAAGAGT TTCGCCACCT TTCCAACACT CACACAAACC ACGAGGACAA GCTACAGGTG 
AAGAACATCA TCTACCATGC TGTGAAGGAT GCTGTGGCAA CACTAAAAGC CCATGAGCCC 
AAATTAGGCC GTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_005173138.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173195.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.

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CloneID	ODa19557
Clone ID Related Accession (Same CDS sequence)	XM_005173138.3 , XM_005173138.4
Accession Version	XM_005173138.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 3B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173138.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTCA GGCTGATAAA 
ATGAAGGGAG GTCTTCCTAA CTTCCTGGAT CATATCATTG CATCAGTAGT GGAGACTAAG 
AAGTCAGAGG TTCGTCGTGC ATCAGGTTCA GCAGAAGTCA TCGAGTCTGC CACTGCTCCT 
CGCAGAGAGG GGGTGATGGG GCTGAGTGTC CTGGATCCAC ACACTTCACA CTCTTGGCTT 
TGTGACGGCC GTTTGCTCTG CCTGCAGGAC CCCAGTAACC CCAACAACTG GAAGATCTTC 
AGAGAGTGCT GGAAACAAGG ACAGCCTGTG CTGGTCTCAG GCATACACAG GAAGCTGAAG 
GAACACTTAT GGAGGCCTGA GGCCTTCAGC GAGGAGTTTG GAGACCAGGA CGTGGACCTG 
GTCAACTGTA GGAACTGCGC CATCATCTCT GATGTTAAAG TCAGGGAATT TTGGGATGGC 
TTCCAGGTCA TTTCCAAGCG ATTGCAAGGT AGTGATGGCC AGCCTATGGT ACTAAAACTT 
AAAGACTGGC CTCCTGGAGA AGATTTTCGA GATATGATGC CCACTCGGTT TAATGATCTC 
ATGGACAATC TTCCTTTGCC CGAGTACACA AAGAGAGATG GCCGTCTCAA CCTGGCCTCT 
CGTCTTCCAA ACTTCTTTGT TCGGCCAGAT CTGGGCCCTA AAATGTACAA TGCGTATGGT 
CTGATCTCCA CAGAAGACAG GAAGGTCGGC ACCACTAACT TGCATTTGGA TGTGTCTGAT 
GCCGTCAACG TTATGGTGTA TGTGGGCATC CCAGAAGGAG AGAATGACCA AGAAAGTGAA 
GCAGACCTCG CTGGATTCAA AGAGGTGATG CAGACTATTG AAGAGGGTGA TGTAGATGAC 
ATGACGAAAA GAAGAGTCTA CGAGATCAAG GAGAAACCTG GGGCTCTCTG GCACATCTAT 
GCTGCCAAAG ACGCTGAGAA GATCCGAGAG CTCTTACGAA AGGTTGGAGA GGAACAAGGT 
CAAGAGAACC CACCAGACCA TGATCCTATC CATGACCAGA GCTGGTACCT GGACCAGACG 
TTGCGTCGCA GGCTGTATGA GGAGTATGGA GTCCAGGGAT GGTCCATTGT GCAGTTTTTG 
GGAGATGCAG TGTTCATCCC AGCTGGAGCA CCACATCAGG TGCACAACCT GTACAGCTGT 
ATCAAAGCTG CAGAAGATTT TGTTTCTCCA GAGCATGTGA AGCACTGTTT TAGACTGACG 
CAAGAGTTTC GCCACCTTTC CAACACTCAC ACAAACCACG AGGACAAGCT ACAGGTGAAG 
AACATCATCT ACCATGCTGT GAAGGATGCT GTGGCAACAC TAAAAGCCCA TGAGCCCAAA 
TTAGGCCGTT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173195.1
CDS	267..5741
Translation	MGDSLGLIGKRLLLLLNDGSSAPAATGGEVERAAWLRGTVRAVSVIGLASPGVEVFVEFEDSPWRKRAWVQLYGDEVRVVLMESAIVWANCSDPSLSTALGSSATQWPALMFKQLIDRVGLGSVVPVEFFGARNLAFLPNGNSLHTFETEKDFTHSLLQEQPALQHAISSWHTDSELQEILRKGSYTIQGRRVKVYQPEFEQPWAFGLVSQHDPVSHIMEITMDQSCLKGEETQVVDPRVIHVMLAEGKLNESQDRRKKEGDGGKGEGSRRRRTASEGDEDITLKRFKGAGEGASDNQNGNSSNRDAEAMVTCVEMPGKGVVEGKDEAGGLGGRVTSTCSPVALSSQDSSNNSNSSQQEHSHIRSTGFVKENGSFIPNQERISSVSAVLPASTPTPPPLKPAPSPFSNTFPSLGQMPNLVPGAPAPKSSPTLPSSEREEGGVLSGYPKTAALVSPGPVTISSPSQENTSSVALTAPVDANQKPSMWGSTSEGNQTPKAPVLAPAGFGKQSSGGVFGNVSTQTNGSSQGDKPFGFSFRGAKDSQRQDSDTSQNLFFQFISQNQSNTQGQSKAFTSLSECLNKEPPSLFKSAAPSEGFKKTVVASASTGLFGSAPASGLAPLKEQPKVPDIKPAGNGILMNKPFGAVGEAQSKLPATFSSAIIQAASSTEVLKPSGLGTSGLGNASGGIRNVNSSTPVSSFGLLAGNKVSDGHENLFLQPSKETNSFLAYGRGVPQTPFGALTSPKSSSAQSASAVSPSGSTSLLGQDPPGSDAKPNLFTMAEPPKGILAPQFAAPALMTTPSFTSVAQDGQQISKPNREGSDDSSGTLLSTEAEGVSVPFEQSKFSLEERLQSIKKDSESSSNSDLSDLSEGEDNAGQSQKPDLPAGNEDKNKAQAAAKSRPRSKPFKVGQSVLKDQNKVRRLKQSGEAFLQDGSCINVAPHLHKCRECRLERYRKSREQDSDDDDPNVACRFFHFRRLAFTKKGVLRVEGFLSPQQSDSMAMGLWLPSITIQEGLDLDTSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPPEVEDGPEEEVFSWLKCAKGQPHEPQNLMPTQIIPGTALYSIGDMVHAARGKWGIKANCPCTSRHKTLMRPSAPNGLSQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASQADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEADLAGFKEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_005173138.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173138.3

ORF Insert Sequence:

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTCA GGCTGATAAA 
ATGAAGGGAG GTCTTCCTAA CTTCCTGGAT CATATCATTG CATCAGTAGT GGAGACTAAG 
AAGTCAGAGG TTCGTCGTGC ATCAGGTTCA GCAGAAGTCA TCGAGTCTGC CACTGCTCCT 
CGCAGAGAGG GGGTGATGGG GCTGAGTGTC CTGGATCCAC ACACTTCACA CTCTTGGCTT 
TGTGACGGCC GTTTGCTCTG CCTGCAGGAC CCCAGTAACC CCAACAACTG GAAGATCTTC 
AGAGAGTGCT GGAAACAAGG ACAGCCTGTG CTGGTCTCAG GCATACACAG GAAGCTGAAG 
GAACACTTAT GGAGGCCTGA GGCCTTCAGC GAGGAGTTTG GAGACCAGGA CGTGGACCTG 
GTCAACTGTA GGAACTGCGC CATCATCTCT GATGTTAAAG TCAGGGAATT TTGGGATGGC 
TTCCAGGTCA TTTCCAAGCG ATTGCAAGGT AGTGATGGCC AGCCTATGGT ACTAAAACTT 
AAAGACTGGC CTCCTGGAGA AGATTTTCGA GATATGATGC CCACTCGGTT TAATGATCTC 
ATGGACAATC TTCCTTTGCC CGAGTACACA AAGAGAGATG GCCGTCTCAA CCTGGCCTCT 
CGTCTTCCAA ACTTCTTTGT TCGGCCAGAT CTGGGCCCTA AAATGTACAA TGCGTATGGT 
CTGATCTCCA CAGAAGACAG GAAGGTCGGC ACCACTAACT TGCATTTGGA TGTGTCTGAT 
GCCGTCAACG TTATGGTGTA TGTGGGCATC CCAGAAGGAG AGAATGACCA AGAAAGTGAA 
GCAGACCTCG CTGGATTCAA AGAGGTGATG CAGACTATTG AAGAGGGTGA TGTAGATGAC 
ATGACGAAAA GAAGAGTCTA CGAGATCAAG GAGAAACCTG GGGCTCTCTG GCACATCTAT 
GCTGCCAAAG ACGCTGAGAA GATCCGAGAG CTCTTACGAA AGGTTGGAGA GGAACAAGGT 
CAAGAGAACC CACCAGACCA TGATCCTATC CATGACCAGA GCTGGTACCT GGACCAGACG 
TTGCGTCGCA GGCTGTATGA GGAGTATGGA GTCCAGGGAT GGTCCATTGT GCAGTTTTTG 
GGAGATGCAG TGTTCATCCC AGCTGGAGCA CCACATCAGG TGCACAACCT GTACAGCTGT 
ATCAAAGCTG CAGAAGATTT TGTTTCTCCA GAGCATGTGA AGCACTGTTT TAGACTGACG 
CAAGAGTTTC GCCACCTTTC CAACACTCAC ACAAACCACG AGGACAAGCT ACAGGTGAAG 
AACATCATCT ACCATGCTGT GAAGGATGCT GTGGCAACAC TAAAAGCCCA TGAGCCCAAA 
TTAGGCCGTT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_005173138.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173195.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.

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CloneID	ODa19557
Clone ID Related Accession (Same CDS sequence)	XM_005173138.3 , XM_005173138.4
Accession Version	XM_005173138.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173138.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTCA GGCTGATAAA 
ATGAAGGGAG GTCTTCCTAA CTTCCTGGAT CATATCATTG CATCAGTAGT GGAGACTAAG 
AAGTCAGAGG TTCGTCGTGC ATCAGGTTCA GCAGAAGTCA TCGAGTCTGC CACTGCTCCT 
CGCAGAGAGG GGGTGATGGG GCTGAGTGTC CTGGATCCAC ACACTTCACA CTCTTGGCTT 
TGTGACGGCC GTTTGCTCTG CCTGCAGGAC CCCAGTAACC CCAACAACTG GAAGATCTTC 
AGAGAGTGCT GGAAACAAGG ACAGCCTGTG CTGGTCTCAG GCATACACAG GAAGCTGAAG 
GAACACTTAT GGAGGCCTGA GGCCTTCAGC GAGGAGTTTG GAGACCAGGA CGTGGACCTG 
GTCAACTGTA GGAACTGCGC CATCATCTCT GATGTTAAAG TCAGGGAATT TTGGGATGGC 
TTCCAGGTCA TTTCCAAGCG ATTGCAAGGT AGTGATGGCC AGCCTATGGT ACTAAAACTT 
AAAGACTGGC CTCCTGGAGA AGATTTTCGA GATATGATGC CCACTCGGTT TAATGATCTC 
ATGGACAATC TTCCTTTGCC CGAGTACACA AAGAGAGATG GCCGTCTCAA CCTGGCCTCT 
CGTCTTCCAA ACTTCTTTGT TCGGCCAGAT CTGGGCCCTA AAATGTACAA TGCGTATGGT 
CTGATCTCCA CAGAAGACAG GAAGGTCGGC ACCACTAACT TGCATTTGGA TGTGTCTGAT 
GCCGTCAACG TTATGGTGTA TGTGGGCATC CCAGAAGGAG AGAATGACCA AGAAAGTGAA 
GCAGACCTCG CTGGATTCAA AGAGGTGATG CAGACTATTG AAGAGGGTGA TGTAGATGAC 
ATGACGAAAA GAAGAGTCTA CGAGATCAAG GAGAAACCTG GGGCTCTCTG GCACATCTAT 
GCTGCCAAAG ACGCTGAGAA GATCCGAGAG CTCTTACGAA AGGTTGGAGA GGAACAAGGT 
CAAGAGAACC CACCAGACCA TGATCCTATC CATGACCAGA GCTGGTACCT GGACCAGACG 
TTGCGTCGCA GGCTGTATGA GGAGTATGGA GTCCAGGGAT GGTCCATTGT GCAGTTTTTG 
GGAGATGCAG TGTTCATCCC AGCTGGAGCA CCACATCAGG TGCACAACCT GTACAGCTGT 
ATCAAAGCTG CAGAAGATTT TGTTTCTCCA GAGCATGTGA AGCACTGTTT TAGACTGACG 
CAAGAGTTTC GCCACCTTTC CAACACTCAC ACAAACCACG AGGACAAGCT ACAGGTGAAG 
AACATCATCT ACCATGCTGT GAAGGATGCT GTGGCAACAC TAAAAGCCCA TGAGCCCAAA 
TTAGGCCGTT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173195.1
CDS	268..5742
Translation	MGDSLGLIGKRLLLLLNDGSSAPAATGGEVERAAWLRGTVRAVSVIGLASPGVEVFVEFEDSPWRKRAWVQLYGDEVRVVLMESAIVWANCSDPSLSTALGSSATQWPALMFKQLIDRVGLGSVVPVEFFGARNLAFLPNGNSLHTFETEKDFTHSLLQEQPALQHAISSWHTDSELQEILRKGSYTIQGRRVKVYQPEFEQPWAFGLVSQHDPVSHIMEITMDQSCLKGEETQVVDPRVIHVMLAEGKLNESQDRRKKEGDGGKGEGSRRRRTASEGDEDITLKRFKGAGEGASDNQNGNSSNRDAEAMVTCVEMPGKGVVEGKDEAGGLGGRVTSTCSPVALSSQDSSNNSNSSQQEHSHIRSTGFVKENGSFIPNQERISSVSAVLPASTPTPPPLKPAPSPFSNTFPSLGQMPNLVPGAPAPKSSPTLPSSEREEGGVLSGYPKTAALVSPGPVTISSPSQENTSSVALTAPVDANQKPSMWGSTSEGNQTPKAPVLAPAGFGKQSSGGVFGNVSTQTNGSSQGDKPFGFSFRGAKDSQRQDSDTSQNLFFQFISQNQSNTQGQSKAFTSLSECLNKEPPSLFKSAAPSEGFKKTVVASASTGLFGSAPASGLAPLKEQPKVPDIKPAGNGILMNKPFGAVGEAQSKLPATFSSAIIQAASSTEVLKPSGLGTSGLGNASGGIRNVNSSTPVSSFGLLAGNKVSDGHENLFLQPSKETNSFLAYGRGVPQTPFGALTSPKSSSAQSASAVSPSGSTSLLGQDPPGSDAKPNLFTMAEPPKGILAPQFAAPALMTTPSFTSVAQDGQQISKPNREGSDDSSGTLLSTEAEGVSVPFEQSKFSLEERLQSIKKDSESSSNSDLSDLSEGEDNAGQSQKPDLPAGNEDKNKAQAAAKSRPRSKPFKVGQSVLKDQNKVRRLKQSGEAFLQDGSCINVAPHLHKCRECRLERYRKSREQDSDDDDPNVACRFFHFRRLAFTKKGVLRVEGFLSPQQSDSMAMGLWLPSITIQEGLDLDTSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPPEVEDGPEEEVFSWLKCAKGQPHEPQNLMPTQIIPGTALYSIGDMVHAARGKWGIKANCPCTSRHKTLMRPSAPNGLSQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASQADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEADLAGFKEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_005173138.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173138.4

ORF Insert Sequence:

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTCA GGCTGATAAA 
ATGAAGGGAG GTCTTCCTAA CTTCCTGGAT CATATCATTG CATCAGTAGT GGAGACTAAG 
AAGTCAGAGG TTCGTCGTGC ATCAGGTTCA GCAGAAGTCA TCGAGTCTGC CACTGCTCCT 
CGCAGAGAGG GGGTGATGGG GCTGAGTGTC CTGGATCCAC ACACTTCACA CTCTTGGCTT 
TGTGACGGCC GTTTGCTCTG CCTGCAGGAC CCCAGTAACC CCAACAACTG GAAGATCTTC 
AGAGAGTGCT GGAAACAAGG ACAGCCTGTG CTGGTCTCAG GCATACACAG GAAGCTGAAG 
GAACACTTAT GGAGGCCTGA GGCCTTCAGC GAGGAGTTTG GAGACCAGGA CGTGGACCTG 
GTCAACTGTA GGAACTGCGC CATCATCTCT GATGTTAAAG TCAGGGAATT TTGGGATGGC 
TTCCAGGTCA TTTCCAAGCG ATTGCAAGGT AGTGATGGCC AGCCTATGGT ACTAAAACTT 
AAAGACTGGC CTCCTGGAGA AGATTTTCGA GATATGATGC CCACTCGGTT TAATGATCTC 
ATGGACAATC TTCCTTTGCC CGAGTACACA AAGAGAGATG GCCGTCTCAA CCTGGCCTCT 
CGTCTTCCAA ACTTCTTTGT TCGGCCAGAT CTGGGCCCTA AAATGTACAA TGCGTATGGT 
CTGATCTCCA CAGAAGACAG GAAGGTCGGC ACCACTAACT TGCATTTGGA TGTGTCTGAT 
GCCGTCAACG TTATGGTGTA TGTGGGCATC CCAGAAGGAG AGAATGACCA AGAAAGTGAA 
GCAGACCTCG CTGGATTCAA AGAGGTGATG CAGACTATTG AAGAGGGTGA TGTAGATGAC 
ATGACGAAAA GAAGAGTCTA CGAGATCAAG GAGAAACCTG GGGCTCTCTG GCACATCTAT 
GCTGCCAAAG ACGCTGAGAA GATCCGAGAG CTCTTACGAA AGGTTGGAGA GGAACAAGGT 
CAAGAGAACC CACCAGACCA TGATCCTATC CATGACCAGA GCTGGTACCT GGACCAGACG 
TTGCGTCGCA GGCTGTATGA GGAGTATGGA GTCCAGGGAT GGTCCATTGT GCAGTTTTTG 
GGAGATGCAG TGTTCATCCC AGCTGGAGCA CCACATCAGG TGCACAACCT GTACAGCTGT 
ATCAAAGCTG CAGAAGATTT TGTTTCTCCA GAGCATGTGA AGCACTGTTT TAGACTGACG 
CAAGAGTTTC GCCACCTTTC CAACACTCAC ACAAACCACG AGGACAAGCT ACAGGTGAAG 
AACATCATCT ACCATGCTGT GAAGGATGCT GTGGCAACAC TAAAAGCCCA TGAGCCCAAA 
TTAGGCCGTT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_005173139.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173196.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.

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CloneID	ODa19558
Clone ID Related Accession (Same CDS sequence)	XM_005173139.3 , XM_005173139.4
Accession Version	XM_005173139.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 3B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173139.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTGC TGATAAAATG 
AAGGGAGGTC TTCCTAACTT CCTGGATCAT ATCATTGCAT CAGTAGTGGA GACTAAGAAG 
TCAGAGGTTC GTCGTGCATC AGGTTCAGCA GAAGTCATCG AGTCTGCCAC TGCTCCTCGC 
AGAGAGGGGG TGATGGGGCT GAGTGTCCTG GATCCACACA CTTCACACTC TTGGCTTTGT 
GACGGCCGTT TGCTCTGCCT GCAGGACCCC AGTAACCCCA ACAACTGGAA GATCTTCAGA 
GAGTGCTGGA AACAAGGACA GCCTGTGCTG GTCTCAGGCA TACACAGGAA GCTGAAGGAA 
CACTTATGGA GGCCTGAGGC CTTCAGCGAG GAGTTTGGAG ACCAGGACGT GGACCTGGTC 
AACTGTAGGA ACTGCGCCAT CATCTCTGAT GTTAAAGTCA GGGAATTTTG GGATGGCTTC 
CAGGTCATTT CCAAGCGATT GCAAGGTAGT GATGGCCAGC CTATGGTACT AAAACTTAAA 
GACTGGCCTC CTGGAGAAGA TTTTCGAGAT ATGATGCCCA CTCGGTTTAA TGATCTCATG 
GACAATCTTC CTTTGCCCGA GTACACAAAG AGAGATGGCC GTCTCAACCT GGCCTCTCGT 
CTTCCAAACT TCTTTGTTCG GCCAGATCTG GGCCCTAAAA TGTACAATGC GTATGGTCTG 
ATCTCCACAG AAGACAGGAA GGTCGGCACC ACTAACTTGC ATTTGGATGT GTCTGATGCC 
GTCAACGTTA TGGTGTATGT GGGCATCCCA GAAGGAGAGA ATGACCAAGA AAGTGAAGCA 
GACCTCGCTG GATTCAAAGA GGTGATGCAG ACTATTGAAG AGGGTGATGT AGATGACATG 
ACGAAAAGAA GAGTCTACGA GATCAAGGAG AAACCTGGGG CTCTCTGGCA CATCTATGCT 
GCCAAAGACG CTGAGAAGAT CCGAGAGCTC TTACGAAAGG TTGGAGAGGA ACAAGGTCAA 
GAGAACCCAC CAGACCATGA TCCTATCCAT GACCAGAGCT GGTACCTGGA CCAGACGTTG 
CGTCGCAGGC TGTATGAGGA GTATGGAGTC CAGGGATGGT CCATTGTGCA GTTTTTGGGA 
GATGCAGTGT TCATCCCAGC TGGAGCACCA CATCAGGTGC ACAACCTGTA CAGCTGTATC 
AAAGCTGCAG AAGATTTTGT TTCTCCAGAG CATGTGAAGC ACTGTTTTAG ACTGACGCAA 
GAGTTTCGCC ACCTTTCCAA CACTCACACA AACCACGAGG ACAAGCTACA GGTGAAGAAC 
ATCATCTACC ATGCTGTGAA GGATGCTGTG GCAACACTAA AAGCCCATGA GCCCAAATTA 
GGCCGTTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173196.1
CDS	267..5738
Translation	MGDSLGLIGKRLLLLLNDGSSAPAATGGEVERAAWLRGTVRAVSVIGLASPGVEVFVEFEDSPWRKRAWVQLYGDEVRVVLMESAIVWANCSDPSLSTALGSSATQWPALMFKQLIDRVGLGSVVPVEFFGARNLAFLPNGNSLHTFETEKDFTHSLLQEQPALQHAISSWHTDSELQEILRKGSYTIQGRRVKVYQPEFEQPWAFGLVSQHDPVSHIMEITMDQSCLKGEETQVVDPRVIHVMLAEGKLNESQDRRKKEGDGGKGEGSRRRRTASEGDEDITLKRFKGAGEGASDNQNGNSSNRDAEAMVTCVEMPGKGVVEGKDEAGGLGGRVTSTCSPVALSSQDSSNNSNSSQQEHSHIRSTGFVKENGSFIPNQERISSVSAVLPASTPTPPPLKPAPSPFSNTFPSLGQMPNLVPGAPAPKSSPTLPSSEREEGGVLSGYPKTAALVSPGPVTISSPSQENTSSVALTAPVDANQKPSMWGSTSEGNQTPKAPVLAPAGFGKQSSGGVFGNVSTQTNGSSQGDKPFGFSFRGAKDSQRQDSDTSQNLFFQFISQNQSNTQGQSKAFTSLSECLNKEPPSLFKSAAPSEGFKKTVVASASTGLFGSAPASGLAPLKEQPKVPDIKPAGNGILMNKPFGAVGEAQSKLPATFSSAIIQAASSTEVLKPSGLGTSGLGNASGGIRNVNSSTPVSSFGLLAGNKVSDGHENLFLQPSKETNSFLAYGRGVPQTPFGALTSPKSSSAQSASAVSPSGSTSLLGQDPPGSDAKPNLFTMAEPPKGILAPQFAAPALMTTPSFTSVAQDGQQISKPNREGSDDSSGTLLSTEAEGVSVPFEQSKFSLEERLQSIKKDSESSSNSDLSDLSEGEDNAGQSQKPDLPAGNEDKNKAQAAAKSRPRSKPFKVGQSVLKDQNKVRRLKQSGEAFLQDGSCINVAPHLHKCRECRLERYRKSREQDSDDDDPNVACRFFHFRRLAFTKKGVLRVEGFLSPQQSDSMAMGLWLPSITIQEGLDLDTSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPPEVEDGPEEEVFSWLKCAKGQPHEPQNLMPTQIIPGTALYSIGDMVHAARGKWGIKANCPCTSRHKTLMRPSAPNGLSQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEADLAGFKEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_005173139.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173139.3

ORF Insert Sequence:

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTGC TGATAAAATG 
AAGGGAGGTC TTCCTAACTT CCTGGATCAT ATCATTGCAT CAGTAGTGGA GACTAAGAAG 
TCAGAGGTTC GTCGTGCATC AGGTTCAGCA GAAGTCATCG AGTCTGCCAC TGCTCCTCGC 
AGAGAGGGGG TGATGGGGCT GAGTGTCCTG GATCCACACA CTTCACACTC TTGGCTTTGT 
GACGGCCGTT TGCTCTGCCT GCAGGACCCC AGTAACCCCA ACAACTGGAA GATCTTCAGA 
GAGTGCTGGA AACAAGGACA GCCTGTGCTG GTCTCAGGCA TACACAGGAA GCTGAAGGAA 
CACTTATGGA GGCCTGAGGC CTTCAGCGAG GAGTTTGGAG ACCAGGACGT GGACCTGGTC 
AACTGTAGGA ACTGCGCCAT CATCTCTGAT GTTAAAGTCA GGGAATTTTG GGATGGCTTC 
CAGGTCATTT CCAAGCGATT GCAAGGTAGT GATGGCCAGC CTATGGTACT AAAACTTAAA 
GACTGGCCTC CTGGAGAAGA TTTTCGAGAT ATGATGCCCA CTCGGTTTAA TGATCTCATG 
GACAATCTTC CTTTGCCCGA GTACACAAAG AGAGATGGCC GTCTCAACCT GGCCTCTCGT 
CTTCCAAACT TCTTTGTTCG GCCAGATCTG GGCCCTAAAA TGTACAATGC GTATGGTCTG 
ATCTCCACAG AAGACAGGAA GGTCGGCACC ACTAACTTGC ATTTGGATGT GTCTGATGCC 
GTCAACGTTA TGGTGTATGT GGGCATCCCA GAAGGAGAGA ATGACCAAGA AAGTGAAGCA 
GACCTCGCTG GATTCAAAGA GGTGATGCAG ACTATTGAAG AGGGTGATGT AGATGACATG 
ACGAAAAGAA GAGTCTACGA GATCAAGGAG AAACCTGGGG CTCTCTGGCA CATCTATGCT 
GCCAAAGACG CTGAGAAGAT CCGAGAGCTC TTACGAAAGG TTGGAGAGGA ACAAGGTCAA 
GAGAACCCAC CAGACCATGA TCCTATCCAT GACCAGAGCT GGTACCTGGA CCAGACGTTG 
CGTCGCAGGC TGTATGAGGA GTATGGAGTC CAGGGATGGT CCATTGTGCA GTTTTTGGGA 
GATGCAGTGT TCATCCCAGC TGGAGCACCA CATCAGGTGC ACAACCTGTA CAGCTGTATC 
AAAGCTGCAG AAGATTTTGT TTCTCCAGAG CATGTGAAGC ACTGTTTTAG ACTGACGCAA 
GAGTTTCGCC ACCTTTCCAA CACTCACACA AACCACGAGG ACAAGCTACA GGTGAAGAAC 
ATCATCTACC ATGCTGTGAA GGATGCTGTG GCAACACTAA AAGCCCATGA GCCCAAATTA 
GGCCGTTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_005173139.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173196.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.

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CloneID	ODa19558
Clone ID Related Accession (Same CDS sequence)	XM_005173139.3 , XM_005173139.4
Accession Version	XM_005173139.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173139.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTGC TGATAAAATG 
AAGGGAGGTC TTCCTAACTT CCTGGATCAT ATCATTGCAT CAGTAGTGGA GACTAAGAAG 
TCAGAGGTTC GTCGTGCATC AGGTTCAGCA GAAGTCATCG AGTCTGCCAC TGCTCCTCGC 
AGAGAGGGGG TGATGGGGCT GAGTGTCCTG GATCCACACA CTTCACACTC TTGGCTTTGT 
GACGGCCGTT TGCTCTGCCT GCAGGACCCC AGTAACCCCA ACAACTGGAA GATCTTCAGA 
GAGTGCTGGA AACAAGGACA GCCTGTGCTG GTCTCAGGCA TACACAGGAA GCTGAAGGAA 
CACTTATGGA GGCCTGAGGC CTTCAGCGAG GAGTTTGGAG ACCAGGACGT GGACCTGGTC 
AACTGTAGGA ACTGCGCCAT CATCTCTGAT GTTAAAGTCA GGGAATTTTG GGATGGCTTC 
CAGGTCATTT CCAAGCGATT GCAAGGTAGT GATGGCCAGC CTATGGTACT AAAACTTAAA 
GACTGGCCTC CTGGAGAAGA TTTTCGAGAT ATGATGCCCA CTCGGTTTAA TGATCTCATG 
GACAATCTTC CTTTGCCCGA GTACACAAAG AGAGATGGCC GTCTCAACCT GGCCTCTCGT 
CTTCCAAACT TCTTTGTTCG GCCAGATCTG GGCCCTAAAA TGTACAATGC GTATGGTCTG 
ATCTCCACAG AAGACAGGAA GGTCGGCACC ACTAACTTGC ATTTGGATGT GTCTGATGCC 
GTCAACGTTA TGGTGTATGT GGGCATCCCA GAAGGAGAGA ATGACCAAGA AAGTGAAGCA 
GACCTCGCTG GATTCAAAGA GGTGATGCAG ACTATTGAAG AGGGTGATGT AGATGACATG 
ACGAAAAGAA GAGTCTACGA GATCAAGGAG AAACCTGGGG CTCTCTGGCA CATCTATGCT 
GCCAAAGACG CTGAGAAGAT CCGAGAGCTC TTACGAAAGG TTGGAGAGGA ACAAGGTCAA 
GAGAACCCAC CAGACCATGA TCCTATCCAT GACCAGAGCT GGTACCTGGA CCAGACGTTG 
CGTCGCAGGC TGTATGAGGA GTATGGAGTC CAGGGATGGT CCATTGTGCA GTTTTTGGGA 
GATGCAGTGT TCATCCCAGC TGGAGCACCA CATCAGGTGC ACAACCTGTA CAGCTGTATC 
AAAGCTGCAG AAGATTTTGT TTCTCCAGAG CATGTGAAGC ACTGTTTTAG ACTGACGCAA 
GAGTTTCGCC ACCTTTCCAA CACTCACACA AACCACGAGG ACAAGCTACA GGTGAAGAAC 
ATCATCTACC ATGCTGTGAA GGATGCTGTG GCAACACTAA AAGCCCATGA GCCCAAATTA 
GGCCGTTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173196.1
CDS	268..5739
Translation	MGDSLGLIGKRLLLLLNDGSSAPAATGGEVERAAWLRGTVRAVSVIGLASPGVEVFVEFEDSPWRKRAWVQLYGDEVRVVLMESAIVWANCSDPSLSTALGSSATQWPALMFKQLIDRVGLGSVVPVEFFGARNLAFLPNGNSLHTFETEKDFTHSLLQEQPALQHAISSWHTDSELQEILRKGSYTIQGRRVKVYQPEFEQPWAFGLVSQHDPVSHIMEITMDQSCLKGEETQVVDPRVIHVMLAEGKLNESQDRRKKEGDGGKGEGSRRRRTASEGDEDITLKRFKGAGEGASDNQNGNSSNRDAEAMVTCVEMPGKGVVEGKDEAGGLGGRVTSTCSPVALSSQDSSNNSNSSQQEHSHIRSTGFVKENGSFIPNQERISSVSAVLPASTPTPPPLKPAPSPFSNTFPSLGQMPNLVPGAPAPKSSPTLPSSEREEGGVLSGYPKTAALVSPGPVTISSPSQENTSSVALTAPVDANQKPSMWGSTSEGNQTPKAPVLAPAGFGKQSSGGVFGNVSTQTNGSSQGDKPFGFSFRGAKDSQRQDSDTSQNLFFQFISQNQSNTQGQSKAFTSLSECLNKEPPSLFKSAAPSEGFKKTVVASASTGLFGSAPASGLAPLKEQPKVPDIKPAGNGILMNKPFGAVGEAQSKLPATFSSAIIQAASSTEVLKPSGLGTSGLGNASGGIRNVNSSTPVSSFGLLAGNKVSDGHENLFLQPSKETNSFLAYGRGVPQTPFGALTSPKSSSAQSASAVSPSGSTSLLGQDPPGSDAKPNLFTMAEPPKGILAPQFAAPALMTTPSFTSVAQDGQQISKPNREGSDDSSGTLLSTEAEGVSVPFEQSKFSLEERLQSIKKDSESSSNSDLSDLSEGEDNAGQSQKPDLPAGNEDKNKAQAAAKSRPRSKPFKVGQSVLKDQNKVRRLKQSGEAFLQDGSCINVAPHLHKCRECRLERYRKSREQDSDDDDPNVACRFFHFRRLAFTKKGVLRVEGFLSPQQSDSMAMGLWLPSITIQEGLDLDTSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPPEVEDGPEEEVFSWLKCAKGQPHEPQNLMPTQIIPGTALYSIGDMVHAARGKWGIKANCPCTSRHKTLMRPSAPNGLSQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEADLAGFKEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_005173139.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173139.4

ORF Insert Sequence:

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTGC TGATAAAATG 
AAGGGAGGTC TTCCTAACTT CCTGGATCAT ATCATTGCAT CAGTAGTGGA GACTAAGAAG 
TCAGAGGTTC GTCGTGCATC AGGTTCAGCA GAAGTCATCG AGTCTGCCAC TGCTCCTCGC 
AGAGAGGGGG TGATGGGGCT GAGTGTCCTG GATCCACACA CTTCACACTC TTGGCTTTGT 
GACGGCCGTT TGCTCTGCCT GCAGGACCCC AGTAACCCCA ACAACTGGAA GATCTTCAGA 
GAGTGCTGGA AACAAGGACA GCCTGTGCTG GTCTCAGGCA TACACAGGAA GCTGAAGGAA 
CACTTATGGA GGCCTGAGGC CTTCAGCGAG GAGTTTGGAG ACCAGGACGT GGACCTGGTC 
AACTGTAGGA ACTGCGCCAT CATCTCTGAT GTTAAAGTCA GGGAATTTTG GGATGGCTTC 
CAGGTCATTT CCAAGCGATT GCAAGGTAGT GATGGCCAGC CTATGGTACT AAAACTTAAA 
GACTGGCCTC CTGGAGAAGA TTTTCGAGAT ATGATGCCCA CTCGGTTTAA TGATCTCATG 
GACAATCTTC CTTTGCCCGA GTACACAAAG AGAGATGGCC GTCTCAACCT GGCCTCTCGT 
CTTCCAAACT TCTTTGTTCG GCCAGATCTG GGCCCTAAAA TGTACAATGC GTATGGTCTG 
ATCTCCACAG AAGACAGGAA GGTCGGCACC ACTAACTTGC ATTTGGATGT GTCTGATGCC 
GTCAACGTTA TGGTGTATGT GGGCATCCCA GAAGGAGAGA ATGACCAAGA AAGTGAAGCA 
GACCTCGCTG GATTCAAAGA GGTGATGCAG ACTATTGAAG AGGGTGATGT AGATGACATG 
ACGAAAAGAA GAGTCTACGA GATCAAGGAG AAACCTGGGG CTCTCTGGCA CATCTATGCT 
GCCAAAGACG CTGAGAAGAT CCGAGAGCTC TTACGAAAGG TTGGAGAGGA ACAAGGTCAA 
GAGAACCCAC CAGACCATGA TCCTATCCAT GACCAGAGCT GGTACCTGGA CCAGACGTTG 
CGTCGCAGGC TGTATGAGGA GTATGGAGTC CAGGGATGGT CCATTGTGCA GTTTTTGGGA 
GATGCAGTGT TCATCCCAGC TGGAGCACCA CATCAGGTGC ACAACCTGTA CAGCTGTATC 
AAAGCTGCAG AAGATTTTGT TTCTCCAGAG CATGTGAAGC ACTGTTTTAG ACTGACGCAA 
GAGTTTCGCC ACCTTTCCAA CACTCACACA AACCACGAGG ACAAGCTACA GGTGAAGAAC 
ATCATCTACC ATGCTGTGAA GGATGCTGTG GCAACACTAA AAGCCCATGA GCCCAAATTA 
GGCCGTTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_005173140.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173197.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X3, mRNA.

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CloneID	ODa19559
Clone ID Related Accession (Same CDS sequence)	XM_005173140.3 , XM_005173140.4
Accession Version	XM_005173140.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 3B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173140.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTCA GGCTGATAAA 
ATGAAGGGAG GTCTTCCTAA CTTCCTGGAT CATATCATTG CATCAGTAGT GGAGACTAAG 
AAGTCAGAGG TTCGTCGTGC ATCAGGTTCA GCAGAAGTCA TCGAGTCTGC CACTGCTCCT 
CGCAGAGAGG GGGTGATGGG GCTGAGTGTC CTGGATCCAC ACACTTCACA CTCTTGGCTT 
TGTGACGGCC GTTTGCTCTG CCTGCAGGAC CCCAGTAACC CCAACAACTG GAAGATCTTC 
AGAGAGTGCT GGAAACAAGG ACAGCCTGTG CTGGTCTCAG GCATACACAG GAAGCTGAAG 
GAACACTTAT GGAGGCCTGA GGCCTTCAGC GAGGAGTTTG GAGACCAGGA CGTGGACCTG 
GTCAACTGTA GGAACTGCGC CATCATCTCT GATGTTAAAG TCAGGGAATT TTGGGATGGC 
TTCCAGGTCA TTTCCAAGCG ATTGCAAGGT AGTGATGGCC AGCCTATGGT ACTAAAACTT 
AAAGACTGGC CTCCTGGAGA AGATTTTCGA GATATGATGC CCACTCGGTT TAATGATCTC 
ATGGACAATC TTCCTTTGCC CGAGTACACA AAGAGAGATG GCCGTCTCAA CCTGGCCTCT 
CGTCTTCCAA ACTTCTTTGT TCGGCCAGAT CTGGGCCCTA AAATGTACAA TGCGTATGGT 
CTGATCTCCA CAGAAGACAG GAAGGTCGGC ACCACTAACT TGCATTTGGA TGTGTCTGAT 
GCCGTCAACG TTATGGTGTA TGTGGGCATC CCAGAAGGAG AGAATGACCA AGAAAGTGAG 
GTGATGCAGA CTATTGAAGA GGGTGATGTA GATGACATGA CGAAAAGAAG AGTCTACGAG 
ATCAAGGAGA AACCTGGGGC TCTCTGGCAC ATCTATGCTG CCAAAGACGC TGAGAAGATC 
CGAGAGCTCT TACGAAAGGT TGGAGAGGAA CAAGGTCAAG AGAACCCACC AGACCATGAT 
CCTATCCATG ACCAGAGCTG GTACCTGGAC CAGACGTTGC GTCGCAGGCT GTATGAGGAG 
TATGGAGTCC AGGGATGGTC CATTGTGCAG TTTTTGGGAG ATGCAGTGTT CATCCCAGCT 
GGAGCACCAC ATCAGGTGCA CAACCTGTAC AGCTGTATCA AAGCTGCAGA AGATTTTGTT 
TCTCCAGAGC ATGTGAAGCA CTGTTTTAGA CTGACGCAAG AGTTTCGCCA CCTTTCCAAC 
ACTCACACAA ACCACGAGGA CAAGCTACAG GTGAAGAACA TCATCTACCA TGCTGTGAAG 
GATGCTGTGG CAACACTAAA AGCCCATGAG CCCAAATTAG GCCGTTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173197.1
CDS	267..5717
Translation	MGDSLGLIGKRLLLLLNDGSSAPAATGGEVERAAWLRGTVRAVSVIGLASPGVEVFVEFEDSPWRKRAWVQLYGDEVRVVLMESAIVWANCSDPSLSTALGSSATQWPALMFKQLIDRVGLGSVVPVEFFGARNLAFLPNGNSLHTFETEKDFTHSLLQEQPALQHAISSWHTDSELQEILRKGSYTIQGRRVKVYQPEFEQPWAFGLVSQHDPVSHIMEITMDQSCLKGEETQVVDPRVIHVMLAEGKLNESQDRRKKEGDGGKGEGSRRRRTASEGDEDITLKRFKGAGEGASDNQNGNSSNRDAEAMVTCVEMPGKGVVEGKDEAGGLGGRVTSTCSPVALSSQDSSNNSNSSQQEHSHIRSTGFVKENGSFIPNQERISSVSAVLPASTPTPPPLKPAPSPFSNTFPSLGQMPNLVPGAPAPKSSPTLPSSEREEGGVLSGYPKTAALVSPGPVTISSPSQENTSSVALTAPVDANQKPSMWGSTSEGNQTPKAPVLAPAGFGKQSSGGVFGNVSTQTNGSSQGDKPFGFSFRGAKDSQRQDSDTSQNLFFQFISQNQSNTQGQSKAFTSLSECLNKEPPSLFKSAAPSEGFKKTVVASASTGLFGSAPASGLAPLKEQPKVPDIKPAGNGILMNKPFGAVGEAQSKLPATFSSAIIQAASSTEVLKPSGLGTSGLGNASGGIRNVNSSTPVSSFGLLAGNKVSDGHENLFLQPSKETNSFLAYGRGVPQTPFGALTSPKSSSAQSASAVSPSGSTSLLGQDPPGSDAKPNLFTMAEPPKGILAPQFAAPALMTTPSFTSVAQDGQQISKPNREGSDDSSGTLLSTEAEGVSVPFEQSKFSLEERLQSIKKDSESSSNSDLSDLSEGEDNAGQSQKPDLPAGNEDKNKAQAAAKSRPRSKPFKVGQSVLKDQNKVRRLKQSGEAFLQDGSCINVAPHLHKCRECRLERYRKSREQDSDDDDPNVACRFFHFRRLAFTKKGVLRVEGFLSPQQSDSMAMGLWLPSITIQEGLDLDTSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPPEVEDGPEEEVFSWLKCAKGQPHEPQNLMPTQIIPGTALYSIGDMVHAARGKWGIKANCPCTSRHKTLMRPSAPNGLSQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASQADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_005173140.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173140.3

ORF Insert Sequence:

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTCA GGCTGATAAA 
ATGAAGGGAG GTCTTCCTAA CTTCCTGGAT CATATCATTG CATCAGTAGT GGAGACTAAG 
AAGTCAGAGG TTCGTCGTGC ATCAGGTTCA GCAGAAGTCA TCGAGTCTGC CACTGCTCCT 
CGCAGAGAGG GGGTGATGGG GCTGAGTGTC CTGGATCCAC ACACTTCACA CTCTTGGCTT 
TGTGACGGCC GTTTGCTCTG CCTGCAGGAC CCCAGTAACC CCAACAACTG GAAGATCTTC 
AGAGAGTGCT GGAAACAAGG ACAGCCTGTG CTGGTCTCAG GCATACACAG GAAGCTGAAG 
GAACACTTAT GGAGGCCTGA GGCCTTCAGC GAGGAGTTTG GAGACCAGGA CGTGGACCTG 
GTCAACTGTA GGAACTGCGC CATCATCTCT GATGTTAAAG TCAGGGAATT TTGGGATGGC 
TTCCAGGTCA TTTCCAAGCG ATTGCAAGGT AGTGATGGCC AGCCTATGGT ACTAAAACTT 
AAAGACTGGC CTCCTGGAGA AGATTTTCGA GATATGATGC CCACTCGGTT TAATGATCTC 
ATGGACAATC TTCCTTTGCC CGAGTACACA AAGAGAGATG GCCGTCTCAA CCTGGCCTCT 
CGTCTTCCAA ACTTCTTTGT TCGGCCAGAT CTGGGCCCTA AAATGTACAA TGCGTATGGT 
CTGATCTCCA CAGAAGACAG GAAGGTCGGC ACCACTAACT TGCATTTGGA TGTGTCTGAT 
GCCGTCAACG TTATGGTGTA TGTGGGCATC CCAGAAGGAG AGAATGACCA AGAAAGTGAG 
GTGATGCAGA CTATTGAAGA GGGTGATGTA GATGACATGA CGAAAAGAAG AGTCTACGAG 
ATCAAGGAGA AACCTGGGGC TCTCTGGCAC ATCTATGCTG CCAAAGACGC TGAGAAGATC 
CGAGAGCTCT TACGAAAGGT TGGAGAGGAA CAAGGTCAAG AGAACCCACC AGACCATGAT 
CCTATCCATG ACCAGAGCTG GTACCTGGAC CAGACGTTGC GTCGCAGGCT GTATGAGGAG 
TATGGAGTCC AGGGATGGTC CATTGTGCAG TTTTTGGGAG ATGCAGTGTT CATCCCAGCT 
GGAGCACCAC ATCAGGTGCA CAACCTGTAC AGCTGTATCA AAGCTGCAGA AGATTTTGTT 
TCTCCAGAGC ATGTGAAGCA CTGTTTTAGA CTGACGCAAG AGTTTCGCCA CCTTTCCAAC 
ACTCACACAA ACCACGAGGA CAAGCTACAG GTGAAGAACA TCATCTACCA TGCTGTGAAG 
GATGCTGTGG CAACACTAAA AGCCCATGAG CCCAAATTAG GCCGTTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_005173140.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173197.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X3, mRNA.

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CloneID	ODa19559
Clone ID Related Accession (Same CDS sequence)	XM_005173140.3 , XM_005173140.4
Accession Version	XM_005173140.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 3B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173140.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTCA GGCTGATAAA 
ATGAAGGGAG GTCTTCCTAA CTTCCTGGAT CATATCATTG CATCAGTAGT GGAGACTAAG 
AAGTCAGAGG TTCGTCGTGC ATCAGGTTCA GCAGAAGTCA TCGAGTCTGC CACTGCTCCT 
CGCAGAGAGG GGGTGATGGG GCTGAGTGTC CTGGATCCAC ACACTTCACA CTCTTGGCTT 
TGTGACGGCC GTTTGCTCTG CCTGCAGGAC CCCAGTAACC CCAACAACTG GAAGATCTTC 
AGAGAGTGCT GGAAACAAGG ACAGCCTGTG CTGGTCTCAG GCATACACAG GAAGCTGAAG 
GAACACTTAT GGAGGCCTGA GGCCTTCAGC GAGGAGTTTG GAGACCAGGA CGTGGACCTG 
GTCAACTGTA GGAACTGCGC CATCATCTCT GATGTTAAAG TCAGGGAATT TTGGGATGGC 
TTCCAGGTCA TTTCCAAGCG ATTGCAAGGT AGTGATGGCC AGCCTATGGT ACTAAAACTT 
AAAGACTGGC CTCCTGGAGA AGATTTTCGA GATATGATGC CCACTCGGTT TAATGATCTC 
ATGGACAATC TTCCTTTGCC CGAGTACACA AAGAGAGATG GCCGTCTCAA CCTGGCCTCT 
CGTCTTCCAA ACTTCTTTGT TCGGCCAGAT CTGGGCCCTA AAATGTACAA TGCGTATGGT 
CTGATCTCCA CAGAAGACAG GAAGGTCGGC ACCACTAACT TGCATTTGGA TGTGTCTGAT 
GCCGTCAACG TTATGGTGTA TGTGGGCATC CCAGAAGGAG AGAATGACCA AGAAAGTGAG 
GTGATGCAGA CTATTGAAGA GGGTGATGTA GATGACATGA CGAAAAGAAG AGTCTACGAG 
ATCAAGGAGA AACCTGGGGC TCTCTGGCAC ATCTATGCTG CCAAAGACGC TGAGAAGATC 
CGAGAGCTCT TACGAAAGGT TGGAGAGGAA CAAGGTCAAG AGAACCCACC AGACCATGAT 
CCTATCCATG ACCAGAGCTG GTACCTGGAC CAGACGTTGC GTCGCAGGCT GTATGAGGAG 
TATGGAGTCC AGGGATGGTC CATTGTGCAG TTTTTGGGAG ATGCAGTGTT CATCCCAGCT 
GGAGCACCAC ATCAGGTGCA CAACCTGTAC AGCTGTATCA AAGCTGCAGA AGATTTTGTT 
TCTCCAGAGC ATGTGAAGCA CTGTTTTAGA CTGACGCAAG AGTTTCGCCA CCTTTCCAAC 
ACTCACACAA ACCACGAGGA CAAGCTACAG GTGAAGAACA TCATCTACCA TGCTGTGAAG 
GATGCTGTGG CAACACTAAA AGCCCATGAG CCCAAATTAG GCCGTTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173197.1
CDS	268..5718
Translation	MGDSLGLIGKRLLLLLNDGSSAPAATGGEVERAAWLRGTVRAVSVIGLASPGVEVFVEFEDSPWRKRAWVQLYGDEVRVVLMESAIVWANCSDPSLSTALGSSATQWPALMFKQLIDRVGLGSVVPVEFFGARNLAFLPNGNSLHTFETEKDFTHSLLQEQPALQHAISSWHTDSELQEILRKGSYTIQGRRVKVYQPEFEQPWAFGLVSQHDPVSHIMEITMDQSCLKGEETQVVDPRVIHVMLAEGKLNESQDRRKKEGDGGKGEGSRRRRTASEGDEDITLKRFKGAGEGASDNQNGNSSNRDAEAMVTCVEMPGKGVVEGKDEAGGLGGRVTSTCSPVALSSQDSSNNSNSSQQEHSHIRSTGFVKENGSFIPNQERISSVSAVLPASTPTPPPLKPAPSPFSNTFPSLGQMPNLVPGAPAPKSSPTLPSSEREEGGVLSGYPKTAALVSPGPVTISSPSQENTSSVALTAPVDANQKPSMWGSTSEGNQTPKAPVLAPAGFGKQSSGGVFGNVSTQTNGSSQGDKPFGFSFRGAKDSQRQDSDTSQNLFFQFISQNQSNTQGQSKAFTSLSECLNKEPPSLFKSAAPSEGFKKTVVASASTGLFGSAPASGLAPLKEQPKVPDIKPAGNGILMNKPFGAVGEAQSKLPATFSSAIIQAASSTEVLKPSGLGTSGLGNASGGIRNVNSSTPVSSFGLLAGNKVSDGHENLFLQPSKETNSFLAYGRGVPQTPFGALTSPKSSSAQSASAVSPSGSTSLLGQDPPGSDAKPNLFTMAEPPKGILAPQFAAPALMTTPSFTSVAQDGQQISKPNREGSDDSSGTLLSTEAEGVSVPFEQSKFSLEERLQSIKKDSESSSNSDLSDLSEGEDNAGQSQKPDLPAGNEDKNKAQAAAKSRPRSKPFKVGQSVLKDQNKVRRLKQSGEAFLQDGSCINVAPHLHKCRECRLERYRKSREQDSDDDDPNVACRFFHFRRLAFTKKGVLRVEGFLSPQQSDSMAMGLWLPSITIQEGLDLDTSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPPEVEDGPEEEVFSWLKCAKGQPHEPQNLMPTQIIPGTALYSIGDMVHAARGKWGIKANCPCTSRHKTLMRPSAPNGLSQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASQADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_005173140.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173140.4

ORF Insert Sequence:

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GTAACATGCG TAGAGATGCC GGGAAAGGGA 
GTAGTGGAGG GAAAAGACGA AGCGGGTGGT TTGGGTGGGA GGGTGACGAG CACCTGTAGT 
CCAGTAGCTT TATCAAGTCA AGACTCCTCT AACAACAGCA ATTCCTCTCA GCAGGAACAC 
TCCCATATAA GGAGCACAGG TTTTGTGAAG GAGAATGGCA GTTTTATCCC AAACCAAGAA 
AGGATTTCTT CAGTATCTGC AGTGCTTCCT GCTTCTACTC CAACGCCACC TCCTTTGAAG 
CCTGCCCCAT CACCTTTTTC TAACACTTTT CCTTCTTTGG GTCAAATGCC CAACTTGGTG 
CCCGGGGCCC CTGCCCCGAA ATCATCTCCC ACACTCCCAT CGTCAGAGAG GGAGGAGGGA 
GGTGTTCTTT CAGGGTATCC TAAAACAGCT GCCCTGGTGT CTCCAGGTCC TGTGACCATT 
TCATCACCTT CCCAAGAAAA CACTTCAAGT GTGGCTCTCA CTGCTCCTGT TGATGCGAAC 
CAAAAGCCCA GTATGTGGGG ATCAACCTCA GAAGGAAATC AGACCCCTAA AGCCCCTGTT 
CTAGCTCCTG CTGGATTTGG TAAACAGTCC AGTGGAGGGG TGTTTGGGAA TGTCTCAACA 
CAGACTAATG GATCTTCCCA GGGGGACAAA CCCTTTGGGT TTTCATTTAG AGGTGCAAAG 
GACTCGCAAA GGCAGGACTC TGATACGTCA CAGAACCTGT TCTTCCAATT CATCTCTCAG 
AACCAGAGCA ACACACAAGG CCAATCAAAA GCCTTCACAT CTTTGTCCGA GTGCCTGAAC 
AAGGAGCCGC CCAGCCTGTT CAAGTCTGCT GCTCCCTCTG AGGGTTTCAA AAAAACTGTT 
GTGGCTTCTG CATCCACTGG ACTGTTTGGT TCAGCACCTG CCAGTGGCCT GGCACCACTG 
AAAGAGCAGC CAAAAGTGCC TGATATCAAG CCTGCAGGCA ATGGGATCCT TATGAACAAG 
CCTTTTGGTG CTGTAGGGGA GGCACAGAGC AAACTCCCAG CCACTTTTTC TTCTGCCATA 
ATTCAAGCTG CAAGCTCTAC AGAGGTGCTG AAACCCTCGG GATTAGGAAC CAGTGGACTT 
GGAAATGCCA GTGGTGGAAT TAGAAATGTA AATAGCTCAA CCCCAGTGAG TAGCTTTGGT 
CTGCTTGCTG GCAACAAGGT TTCCGATGGA CACGAGAACC TGTTCTTGCA GCCCTCGAAG 
GAGACAAATT CGTTTCTTGC CTATGGCAGA GGTGTTCCGC AAACCCCTTT TGGTGCATTG 
ACATCTCCAA AGTCTTCATC TGCCCAGTCC GCATCTGCTG TTTCACCTTC AGGCAGCACC 
AGTCTGCTCG GTCAAGATCC TCCTGGTAGT GATGCAAAAC CAAATCTCTT CACTATGGCA 
GAACCTCCCA AGGGAATCTT AGCCCCTCAG TTTGCAGCTC CTGCTCTCAT GACCACTCCG 
TCATTCACAT CAGTTGCACA GGATGGACAG CAAATATCCA AACCAAACAG AGAAGGATCA 
GATGACAGCT CTGGAACCCT TCTCAGCACT GAAGCAGAAG GTGTCTCTGT GCCCTTTGAG 
CAAAGCAAGT TTTCATTGGA GGAACGTCTT CAGTCTATCA AAAAGGATTC AGAGTCCAGT 
AGTAACAGTG ACTTGTCTGA TTTGAGTGAG GGAGAGGACA ACGCTGGCCA GAGCCAGAAA 
CCCGATCTTC CGGCAGGCAA TGAGGATAAG AACAAGGCCC AGGCTGCAGC CAAGAGCCGG 
CCACGGAGCA AACCATTTAA AGTTGGACAG TCTGTGTTGA AGGACCAGAA TAAGGTACGG 
CGCTTGAAGC AGTCTGGAGA GGCCTTTCTA CAAGATGGCT CCTGCATTAA CGTGGCTCCC 
CACTTGCACA AATGTCGTGA GTGCCGTCTG GAGCGCTACA GGAAATCGCG TGAGCAGGAC 
TCTGATGATG ACGACCCAAA TGTAGCCTGT CGATTCTTTC ACTTTCGCAG GTTGGCTTTC 
ACTAAAAAGG GAGTTCTTCG TGTGGAGGGT TTTCTGAGCC CACAGCAGAG TGACAGCATG 
GCAATGGGCC TGTGGCTTCC TTCCATCACA ATACAAGAAG GACTTGACCT GGACACGTCC 
AAATATATTT TAGCAAATGT GGGGGACCAG TTTTGCCAAT TGGTCATGTC AGAGAAAGAA 
GCCATGATGA TGGTAGAACC ACATCAAAAA GTGGCTTGGA AGCGAGCTGT CCGAGGAGTG 
AGAGAAATGT GTGATGTTTG TGAAACAACC CTGTTCAACA TCCACTGGGT TTGTCGTAAA 
TGTGGCTTCG GCGTTTGTCT TGACTGCTAT CGACTACGCA AGAACAGGCC ACCTGAAGTG 
GAAGATGGTC CTGAAGAGGA GGTGTTCTCA TGGCTAAAGT GTGCTAAAGG CCAGCCACAC 
GAACCACAGA ACCTCATGCC TACTCAAATT ATACCCGGCA CGGCATTGTA CAGCATAGGT 
GACATGGTAC ATGCAGCCAG AGGTAAATGG GGGATTAAAG CAAACTGTCC CTGCACTAGT 
CGGCACAAAA CGTTGATGCG GCCCAGTGCT CCAAATGGTC TTTCACAGTC GGGTGCAGCT 
CACAGTTCGG GGAATGGAAC CACGTCTACT TCATCCACAA CCCGTCCAAA TGAAGAATCT 
GCAGCTGGTG TTGTAGTCAA AACTGAACCC GTTGAAGAGC CTACAAGTGC TGATACCACA 
TCAAGTACCA GTGGGACAAA TAGCAGCACA TCTAGCCCTG CTCCTGCTCC AACTTCTGCT 
CCTACTCTTC TTCCTCCTTC TCTTCCTCCT CCTTCACTTC CTCCTCCTTC ACTTCCTCCT 
GCTCCTGCAC TTGTACCTAC ACCTCCTGCA AAGGATAGCA AGAGCAGTTC TTCAGCACTT 
CACTGGCTGG CAGATCTGGC CACACAAAAG GAGCCCAAAG AGTCTCTGCG CTCCATGATG 
GGTCGTGACT CTCGTTCTCC ATTTGGCCTG GACTCGTTCA GCACACTTTC CAAACCATCA 
GGGTCCAGTC CTAAGCTGTT CAACAGTCTG CTGCTGGGTG CAGGCCCTTC ACAACCCAAA 
GCAGAGGGAA CCAGCCTCCG AGATCTGCTG AACTCTGGCT CTGGCAAACT CCCGCAAGGG 
CCCACAGAGG GAGGTGTCCC ATTTCCTCCA GTGTTCTCCA CTGCTAGTCA GGCTGATAAA 
ATGAAGGGAG GTCTTCCTAA CTTCCTGGAT CATATCATTG CATCAGTAGT GGAGACTAAG 
AAGTCAGAGG TTCGTCGTGC ATCAGGTTCA GCAGAAGTCA TCGAGTCTGC CACTGCTCCT 
CGCAGAGAGG GGGTGATGGG GCTGAGTGTC CTGGATCCAC ACACTTCACA CTCTTGGCTT 
TGTGACGGCC GTTTGCTCTG CCTGCAGGAC CCCAGTAACC CCAACAACTG GAAGATCTTC 
AGAGAGTGCT GGAAACAAGG ACAGCCTGTG CTGGTCTCAG GCATACACAG GAAGCTGAAG 
GAACACTTAT GGAGGCCTGA GGCCTTCAGC GAGGAGTTTG GAGACCAGGA CGTGGACCTG 
GTCAACTGTA GGAACTGCGC CATCATCTCT GATGTTAAAG TCAGGGAATT TTGGGATGGC 
TTCCAGGTCA TTTCCAAGCG ATTGCAAGGT AGTGATGGCC AGCCTATGGT ACTAAAACTT 
AAAGACTGGC CTCCTGGAGA AGATTTTCGA GATATGATGC CCACTCGGTT TAATGATCTC 
ATGGACAATC TTCCTTTGCC CGAGTACACA AAGAGAGATG GCCGTCTCAA CCTGGCCTCT 
CGTCTTCCAA ACTTCTTTGT TCGGCCAGAT CTGGGCCCTA AAATGTACAA TGCGTATGGT 
CTGATCTCCA CAGAAGACAG GAAGGTCGGC ACCACTAACT TGCATTTGGA TGTGTCTGAT 
GCCGTCAACG TTATGGTGTA TGTGGGCATC CCAGAAGGAG AGAATGACCA AGAAAGTGAG 
GTGATGCAGA CTATTGAAGA GGGTGATGTA GATGACATGA CGAAAAGAAG AGTCTACGAG 
ATCAAGGAGA AACCTGGGGC TCTCTGGCAC ATCTATGCTG CCAAAGACGC TGAGAAGATC 
CGAGAGCTCT TACGAAAGGT TGGAGAGGAA CAAGGTCAAG AGAACCCACC AGACCATGAT 
CCTATCCATG ACCAGAGCTG GTACCTGGAC CAGACGTTGC GTCGCAGGCT GTATGAGGAG 
TATGGAGTCC AGGGATGGTC CATTGTGCAG TTTTTGGGAG ATGCAGTGTT CATCCCAGCT 
GGAGCACCAC ATCAGGTGCA CAACCTGTAC AGCTGTATCA AAGCTGCAGA AGATTTTGTT 
TCTCCAGAGC ATGTGAAGCA CTGTTTTAGA CTGACGCAAG AGTTTCGCCA CCTTTCCAAC 
ACTCACACAA ACCACGAGGA CAAGCTACAG GTGAAGAACA TCATCTACCA TGCTGTGAAG 
GATGCTGTGG CAACACTAAA AGCCCATGAG CCCAAATTAG GCCGTTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_005173141.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173198.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X4, mRNA.

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CloneID	ODa19560
Clone ID Related Accession (Same CDS sequence)	XM_005173141.3 , XM_005173141.4
Accession Version	XM_005173141.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 3B isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG CAGGAACACT CCCATATAAG GAGCACAGGT 
TTTGTGAAGG AGAATGGCAG TTTTATCCCA AACCAAGAAA GGATTTCTTC AGTATCTGCA 
GTGCTTCCTG CTTCTACTCC AACGCCACCT CCTTTGAAGC CTGCCCCATC ACCTTTTTCT 
AACACTTTTC CTTCTTTGGG TCAAATGCCC AACTTGGTGC CCGGGGCCCC TGCCCCGAAA 
TCATCTCCCA CACTCCCATC GTCAGAGAGG GAGGAGGGAG GTGTTCTTTC AGGGTATCCT 
AAAACAGCTG CCCTGGTGTC TCCAGGTCCT GTGACCATTT CATCACCTTC CCAAGAAAAC 
ACTTCAAGTG TGGCTCTCAC TGCTCCTGTT GATGCGAACC AAAAGCCCAG TATGTGGGGA 
TCAACCTCAG AAGGAAATCA GACCCCTAAA GCCCCTGTTC TAGCTCCTGC TGGATTTGGT 
AAACAGTCCA GTGGAGGGGT GTTTGGGAAT GTCTCAACAC AGACTAATGG ATCTTCCCAG 
GGGGACAAAC CCTTTGGGTT TTCATTTAGA GGTGCAAAGG ACTCGCAAAG GCAGGACTCT 
GATACGTCAC AGAACCTGTT CTTCCAATTC ATCTCTCAGA ACCAGAGCAA CACACAAGGC 
CAATCAAAAG CCTTCACATC TTTGTCCGAG TGCCTGAACA AGGAGCCGCC CAGCCTGTTC 
AAGTCTGCTG CTCCCTCTGA GGGTTTCAAA AAAACTGTTG TGGCTTCTGC ATCCACTGGA 
CTGTTTGGTT CAGCACCTGC CAGTGGCCTG GCACCACTGA AAGAGCAGCC AAAAGTGCCT 
GATATCAAGC CTGCAGGCAA TGGGATCCTT ATGAACAAGC CTTTTGGTGC TGTAGGGGAG 
GCACAGAGCA AACTCCCAGC CACTTTTTCT TCTGCCATAA TTCAAGCTGC AAGCTCTACA 
GAGGTGCTGA AACCCTCGGG ATTAGGAACC AGTGGACTTG GAAATGCCAG TGGTGGAATT 
AGAAATGTAA ATAGCTCAAC CCCAGTGAGT AGCTTTGGTC TGCTTGCTGG CAACAAGGTT 
TCCGATGGAC ACGAGAACCT GTTCTTGCAG CCCTCGAAGG AGACAAATTC GTTTCTTGCC 
TATGGCAGAG GTGTTCCGCA AACCCCTTTT GGTGCATTGA CATCTCCAAA GTCTTCATCT 
GCCCAGTCCG CATCTGCTGT TTCACCTTCA GGCAGCACCA GTCTGCTCGG TCAAGATCCT 
CCTGGTAGTG ATGCAAAACC AAATCTCTTC ACTATGGCAG AACCTCCCAA GGGAATCTTA 
GCCCCTCAGT TTGCAGCTCC TGCTCTCATG ACCACTCCGT CATTCACATC AGTTGCACAG 
GATGGACAGC AAATATCCAA ACCAAACAGA GAAGGATCAG ATGACAGCTC TGGAACCCTT 
CTCAGCACTG AAGCAGAAGG TGTCTCTGTG CCCTTTGAGC AAAGCAAGTT TTCATTGGAG 
GAACGTCTTC AGTCTATCAA AAAGGATTCA GAGTCCAGTA GTAACAGTGA CTTGTCTGAT 
TTGAGTGAGG GAGAGGACAA CGCTGGCCAG AGCCAGAAAC CCGATCTTCC GGCAGGCAAT 
GAGGATAAGA ACAAGGCCCA GGCTGCAGCC AAGAGCCGGC CACGGAGCAA ACCATTTAAA 
GTTGGACAGT CTGTGTTGAA GGACCAGAAT AAGGTACGGC GCTTGAAGCA GTCTGGAGAG 
GCCTTTCTAC AAGATGGCTC CTGCATTAAC GTGGCTCCCC ACTTGCACAA ATGTCGTGAG 
TGCCGTCTGG AGCGCTACAG GAAATCGCGT GAGCAGGACT CTGATGATGA CGACCCAAAT 
GTAGCCTGTC GATTCTTTCA CTTTCGCAGG TTGGCTTTCA CTAAAAAGGG AGTTCTTCGT 
GTGGAGGGTT TTCTGAGCCC ACAGCAGAGT GACAGCATGG CAATGGGCCT GTGGCTTCCT 
TCCATCACAA TACAAGAAGG ACTTGACCTG GACACGTCCA AATATATTTT AGCAAATGTG 
GGGGACCAGT TTTGCCAATT GGTCATGTCA GAGAAAGAAG CCATGATGAT GGTAGAACCA 
CATCAAAAAG TGGCTTGGAA GCGAGCTGTC CGAGGAGTGA GAGAAATGTG TGATGTTTGT 
GAAACAACCC TGTTCAACAT CCACTGGGTT TGTCGTAAAT GTGGCTTCGG CGTTTGTCTT 
GACTGCTATC GACTACGCAA GAACAGGCCA CCTGAAGTGG AAGATGGTCC TGAAGAGGAG 
GTGTTCTCAT GGCTAAAGTG TGCTAAAGGC CAGCCACACG AACCACAGAA CCTCATGCCT 
ACTCAAATTA TACCCGGCAC GGCATTGTAC AGCATAGGTG ACATGGTACA TGCAGCCAGA 
GGTAAATGGG GGATTAAAGC AAACTGTCCC TGCACTAGTC GGCACAAAAC GTTGATGCGG 
CCCAGTGCTC CAAATGGTCT TTCACAGTCG GGTGCAGCTC ACAGTTCGGG GAATGGAACC 
ACGTCTACTT CATCCACAAC CCGTCCAAAT GAAGAATCTG CAGCTGGTGT TGTAGTCAAA 
ACTGAACCCG TTGAAGAGCC TACAAGTGCT GATACCACAT CAAGTACCAG TGGGACAAAT 
AGCAGCACAT CTAGCCCTGC TCCTGCTCCA ACTTCTGCTC CTACTCTTCT TCCTCCTTCT 
CTTCCTCCTC CTTCACTTCC TCCTCCTTCA CTTCCTCCTG CTCCTGCACT TGTACCTACA 
CCTCCTGCAA AGGATAGCAA GAGCAGTTCT TCAGCACTTC ACTGGCTGGC AGATCTGGCC 
ACACAAAAGG AGCCCAAAGA GTCTCTGCGC TCCATGATGG GTCGTGACTC TCGTTCTCCA 
TTTGGCCTGG ACTCGTTCAG CACACTTTCC AAACCATCAG GGTCCAGTCC TAAGCTGTTC 
AACAGTCTGC TGCTGGGTGC AGGCCCTTCA CAACCCAAAG CAGAGGGAAC CAGCCTCCGA 
GATCTGCTGA ACTCTGGCTC TGGCAAACTC CCGCAAGGGC CCACAGAGGG AGGTGTCCCA 
TTTCCTCCAG TGTTCTCCAC TGCTAGTCAG GCTGATAAAA TGAAGGGAGG TCTTCCTAAC 
TTCCTGGATC ATATCATTGC ATCAGTAGTG GAGACTAAGA AGTCAGAGGT TCGTCGTGCA 
TCAGGTTCAG CAGAAGTCAT CGAGTCTGCC ACTGCTCCTC GCAGAGAGGG GGTGATGGGG 
CTGAGTGTCC TGGATCCACA CACTTCACAC TCTTGGCTTT GTGACGGCCG TTTGCTCTGC 
CTGCAGGACC CCAGTAACCC CAACAACTGG AAGATCTTCA GAGAGTGCTG GAAACAAGGA 
CAGCCTGTGC TGGTCTCAGG CATACACAGG AAGCTGAAGG AACACTTATG GAGGCCTGAG 
GCCTTCAGCG AGGAGTTTGG AGACCAGGAC GTGGACCTGG TCAACTGTAG GAACTGCGCC 
ATCATCTCTG ATGTTAAAGT CAGGGAATTT TGGGATGGCT TCCAGGTCAT TTCCAAGCGA 
TTGCAAGGTA GTGATGGCCA GCCTATGGTA CTAAAACTTA AAGACTGGCC TCCTGGAGAA 
GATTTTCGAG ATATGATGCC CACTCGGTTT AATGATCTCA TGGACAATCT TCCTTTGCCC 
GAGTACACAA AGAGAGATGG CCGTCTCAAC CTGGCCTCTC GTCTTCCAAA CTTCTTTGTT 
CGGCCAGATC TGGGCCCTAA AATGTACAAT GCGTATGGTC TGATCTCCAC AGAAGACAGG 
AAGGTCGGCA CCACTAACTT GCATTTGGAT GTGTCTGATG CCGTCAACGT TATGGTGTAT 
GTGGGCATCC CAGAAGGAGA GAATGACCAA GAAAGTGAAG CAGACCTCGC TGGATTCAAA 
GAGGTGATGC AGACTATTGA AGAGGGTGAT GTAGATGACA TGACGAAAAG AAGAGTCTAC 
GAGATCAAGG AGAAACCTGG GGCTCTCTGG CACATCTATG CTGCCAAAGA CGCTGAGAAG 
ATCCGAGAGC TCTTACGAAA GGTTGGAGAG GAACAAGGTC AAGAGAACCC ACCAGACCAT 
GATCCTATCC ATGACCAGAG CTGGTACCTG GACCAGACGT TGCGTCGCAG GCTGTATGAG 
GAGTATGGAG TCCAGGGATG GTCCATTGTG CAGTTTTTGG GAGATGCAGT GTTCATCCCA 
GCTGGAGCAC CACATCAGGT GCACAACCTG TACAGCTGTA TCAAAGCTGC AGAAGATTTT 
GTTTCTCCAG AGCATGTGAA GCACTGTTTT AGACTGACGC AAGAGTTTCG CCACCTTTCC 
AACACTCACA CAAACCACGA GGACAAGCTA CAGGTGAAGA ACATCATCTA CCATGCTGTG 
AAGGATGCTG TGGCAACACT AAAAGCCCAT GAGCCCAAAT TAGGCCGTTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173198.1
CDS	266..5599
Translation	MGDSLGLIGKRLLLLLNDGSSAPAATGGEVERAAWLRGTVRAVSVIGLASPGVEVFVEFEDSPWRKRAWVQLYGDEVRVVLMESAIVWANCSDPSLSTALGSSATQWPALMFKQLIDRVGLGSVVPVEFFGARNLAFLPNGNSLHTFETEKDFTHSLLQEQPALQHAISSWHTDSELQEILRKGSYTIQGRRVKVYQPEFEQPWAFGLVSQHDPVSHIMEITMDQSCLKGEETQVVDPRVIHVMLAEGKLNESQDRRKKEGDGGKGEGSRRRRTASEGDEDITLKRFKGAGEGASDNQNGNSSNRDAEAMQEHSHIRSTGFVKENGSFIPNQERISSVSAVLPASTPTPPPLKPAPSPFSNTFPSLGQMPNLVPGAPAPKSSPTLPSSEREEGGVLSGYPKTAALVSPGPVTISSPSQENTSSVALTAPVDANQKPSMWGSTSEGNQTPKAPVLAPAGFGKQSSGGVFGNVSTQTNGSSQGDKPFGFSFRGAKDSQRQDSDTSQNLFFQFISQNQSNTQGQSKAFTSLSECLNKEPPSLFKSAAPSEGFKKTVVASASTGLFGSAPASGLAPLKEQPKVPDIKPAGNGILMNKPFGAVGEAQSKLPATFSSAIIQAASSTEVLKPSGLGTSGLGNASGGIRNVNSSTPVSSFGLLAGNKVSDGHENLFLQPSKETNSFLAYGRGVPQTPFGALTSPKSSSAQSASAVSPSGSTSLLGQDPPGSDAKPNLFTMAEPPKGILAPQFAAPALMTTPSFTSVAQDGQQISKPNREGSDDSSGTLLSTEAEGVSVPFEQSKFSLEERLQSIKKDSESSSNSDLSDLSEGEDNAGQSQKPDLPAGNEDKNKAQAAAKSRPRSKPFKVGQSVLKDQNKVRRLKQSGEAFLQDGSCINVAPHLHKCRECRLERYRKSREQDSDDDDPNVACRFFHFRRLAFTKKGVLRVEGFLSPQQSDSMAMGLWLPSITIQEGLDLDTSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPPEVEDGPEEEVFSWLKCAKGQPHEPQNLMPTQIIPGTALYSIGDMVHAARGKWGIKANCPCTSRHKTLMRPSAPNGLSQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASQADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEADLAGFKEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_005173141.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173141.3

ORF Insert Sequence:

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG CAGGAACACT CCCATATAAG GAGCACAGGT 
TTTGTGAAGG AGAATGGCAG TTTTATCCCA AACCAAGAAA GGATTTCTTC AGTATCTGCA 
GTGCTTCCTG CTTCTACTCC AACGCCACCT CCTTTGAAGC CTGCCCCATC ACCTTTTTCT 
AACACTTTTC CTTCTTTGGG TCAAATGCCC AACTTGGTGC CCGGGGCCCC TGCCCCGAAA 
TCATCTCCCA CACTCCCATC GTCAGAGAGG GAGGAGGGAG GTGTTCTTTC AGGGTATCCT 
AAAACAGCTG CCCTGGTGTC TCCAGGTCCT GTGACCATTT CATCACCTTC CCAAGAAAAC 
ACTTCAAGTG TGGCTCTCAC TGCTCCTGTT GATGCGAACC AAAAGCCCAG TATGTGGGGA 
TCAACCTCAG AAGGAAATCA GACCCCTAAA GCCCCTGTTC TAGCTCCTGC TGGATTTGGT 
AAACAGTCCA GTGGAGGGGT GTTTGGGAAT GTCTCAACAC AGACTAATGG ATCTTCCCAG 
GGGGACAAAC CCTTTGGGTT TTCATTTAGA GGTGCAAAGG ACTCGCAAAG GCAGGACTCT 
GATACGTCAC AGAACCTGTT CTTCCAATTC ATCTCTCAGA ACCAGAGCAA CACACAAGGC 
CAATCAAAAG CCTTCACATC TTTGTCCGAG TGCCTGAACA AGGAGCCGCC CAGCCTGTTC 
AAGTCTGCTG CTCCCTCTGA GGGTTTCAAA AAAACTGTTG TGGCTTCTGC ATCCACTGGA 
CTGTTTGGTT CAGCACCTGC CAGTGGCCTG GCACCACTGA AAGAGCAGCC AAAAGTGCCT 
GATATCAAGC CTGCAGGCAA TGGGATCCTT ATGAACAAGC CTTTTGGTGC TGTAGGGGAG 
GCACAGAGCA AACTCCCAGC CACTTTTTCT TCTGCCATAA TTCAAGCTGC AAGCTCTACA 
GAGGTGCTGA AACCCTCGGG ATTAGGAACC AGTGGACTTG GAAATGCCAG TGGTGGAATT 
AGAAATGTAA ATAGCTCAAC CCCAGTGAGT AGCTTTGGTC TGCTTGCTGG CAACAAGGTT 
TCCGATGGAC ACGAGAACCT GTTCTTGCAG CCCTCGAAGG AGACAAATTC GTTTCTTGCC 
TATGGCAGAG GTGTTCCGCA AACCCCTTTT GGTGCATTGA CATCTCCAAA GTCTTCATCT 
GCCCAGTCCG CATCTGCTGT TTCACCTTCA GGCAGCACCA GTCTGCTCGG TCAAGATCCT 
CCTGGTAGTG ATGCAAAACC AAATCTCTTC ACTATGGCAG AACCTCCCAA GGGAATCTTA 
GCCCCTCAGT TTGCAGCTCC TGCTCTCATG ACCACTCCGT CATTCACATC AGTTGCACAG 
GATGGACAGC AAATATCCAA ACCAAACAGA GAAGGATCAG ATGACAGCTC TGGAACCCTT 
CTCAGCACTG AAGCAGAAGG TGTCTCTGTG CCCTTTGAGC AAAGCAAGTT TTCATTGGAG 
GAACGTCTTC AGTCTATCAA AAAGGATTCA GAGTCCAGTA GTAACAGTGA CTTGTCTGAT 
TTGAGTGAGG GAGAGGACAA CGCTGGCCAG AGCCAGAAAC CCGATCTTCC GGCAGGCAAT 
GAGGATAAGA ACAAGGCCCA GGCTGCAGCC AAGAGCCGGC CACGGAGCAA ACCATTTAAA 
GTTGGACAGT CTGTGTTGAA GGACCAGAAT AAGGTACGGC GCTTGAAGCA GTCTGGAGAG 
GCCTTTCTAC AAGATGGCTC CTGCATTAAC GTGGCTCCCC ACTTGCACAA ATGTCGTGAG 
TGCCGTCTGG AGCGCTACAG GAAATCGCGT GAGCAGGACT CTGATGATGA CGACCCAAAT 
GTAGCCTGTC GATTCTTTCA CTTTCGCAGG TTGGCTTTCA CTAAAAAGGG AGTTCTTCGT 
GTGGAGGGTT TTCTGAGCCC ACAGCAGAGT GACAGCATGG CAATGGGCCT GTGGCTTCCT 
TCCATCACAA TACAAGAAGG ACTTGACCTG GACACGTCCA AATATATTTT AGCAAATGTG 
GGGGACCAGT TTTGCCAATT GGTCATGTCA GAGAAAGAAG CCATGATGAT GGTAGAACCA 
CATCAAAAAG TGGCTTGGAA GCGAGCTGTC CGAGGAGTGA GAGAAATGTG TGATGTTTGT 
GAAACAACCC TGTTCAACAT CCACTGGGTT TGTCGTAAAT GTGGCTTCGG CGTTTGTCTT 
GACTGCTATC GACTACGCAA GAACAGGCCA CCTGAAGTGG AAGATGGTCC TGAAGAGGAG 
GTGTTCTCAT GGCTAAAGTG TGCTAAAGGC CAGCCACACG AACCACAGAA CCTCATGCCT 
ACTCAAATTA TACCCGGCAC GGCATTGTAC AGCATAGGTG ACATGGTACA TGCAGCCAGA 
GGTAAATGGG GGATTAAAGC AAACTGTCCC TGCACTAGTC GGCACAAAAC GTTGATGCGG 
CCCAGTGCTC CAAATGGTCT TTCACAGTCG GGTGCAGCTC ACAGTTCGGG GAATGGAACC 
ACGTCTACTT CATCCACAAC CCGTCCAAAT GAAGAATCTG CAGCTGGTGT TGTAGTCAAA 
ACTGAACCCG TTGAAGAGCC TACAAGTGCT GATACCACAT CAAGTACCAG TGGGACAAAT 
AGCAGCACAT CTAGCCCTGC TCCTGCTCCA ACTTCTGCTC CTACTCTTCT TCCTCCTTCT 
CTTCCTCCTC CTTCACTTCC TCCTCCTTCA CTTCCTCCTG CTCCTGCACT TGTACCTACA 
CCTCCTGCAA AGGATAGCAA GAGCAGTTCT TCAGCACTTC ACTGGCTGGC AGATCTGGCC 
ACACAAAAGG AGCCCAAAGA GTCTCTGCGC TCCATGATGG GTCGTGACTC TCGTTCTCCA 
TTTGGCCTGG ACTCGTTCAG CACACTTTCC AAACCATCAG GGTCCAGTCC TAAGCTGTTC 
AACAGTCTGC TGCTGGGTGC AGGCCCTTCA CAACCCAAAG CAGAGGGAAC CAGCCTCCGA 
GATCTGCTGA ACTCTGGCTC TGGCAAACTC CCGCAAGGGC CCACAGAGGG AGGTGTCCCA 
TTTCCTCCAG TGTTCTCCAC TGCTAGTCAG GCTGATAAAA TGAAGGGAGG TCTTCCTAAC 
TTCCTGGATC ATATCATTGC ATCAGTAGTG GAGACTAAGA AGTCAGAGGT TCGTCGTGCA 
TCAGGTTCAG CAGAAGTCAT CGAGTCTGCC ACTGCTCCTC GCAGAGAGGG GGTGATGGGG 
CTGAGTGTCC TGGATCCACA CACTTCACAC TCTTGGCTTT GTGACGGCCG TTTGCTCTGC 
CTGCAGGACC CCAGTAACCC CAACAACTGG AAGATCTTCA GAGAGTGCTG GAAACAAGGA 
CAGCCTGTGC TGGTCTCAGG CATACACAGG AAGCTGAAGG AACACTTATG GAGGCCTGAG 
GCCTTCAGCG AGGAGTTTGG AGACCAGGAC GTGGACCTGG TCAACTGTAG GAACTGCGCC 
ATCATCTCTG ATGTTAAAGT CAGGGAATTT TGGGATGGCT TCCAGGTCAT TTCCAAGCGA 
TTGCAAGGTA GTGATGGCCA GCCTATGGTA CTAAAACTTA AAGACTGGCC TCCTGGAGAA 
GATTTTCGAG ATATGATGCC CACTCGGTTT AATGATCTCA TGGACAATCT TCCTTTGCCC 
GAGTACACAA AGAGAGATGG CCGTCTCAAC CTGGCCTCTC GTCTTCCAAA CTTCTTTGTT 
CGGCCAGATC TGGGCCCTAA AATGTACAAT GCGTATGGTC TGATCTCCAC AGAAGACAGG 
AAGGTCGGCA CCACTAACTT GCATTTGGAT GTGTCTGATG CCGTCAACGT TATGGTGTAT 
GTGGGCATCC CAGAAGGAGA GAATGACCAA GAAAGTGAAG CAGACCTCGC TGGATTCAAA 
GAGGTGATGC AGACTATTGA AGAGGGTGAT GTAGATGACA TGACGAAAAG AAGAGTCTAC 
GAGATCAAGG AGAAACCTGG GGCTCTCTGG CACATCTATG CTGCCAAAGA CGCTGAGAAG 
ATCCGAGAGC TCTTACGAAA GGTTGGAGAG GAACAAGGTC AAGAGAACCC ACCAGACCAT 
GATCCTATCC ATGACCAGAG CTGGTACCTG GACCAGACGT TGCGTCGCAG GCTGTATGAG 
GAGTATGGAG TCCAGGGATG GTCCATTGTG CAGTTTTTGG GAGATGCAGT GTTCATCCCA 
GCTGGAGCAC CACATCAGGT GCACAACCTG TACAGCTGTA TCAAAGCTGC AGAAGATTTT 
GTTTCTCCAG AGCATGTGAA GCACTGTTTT AGACTGACGC AAGAGTTTCG CCACCTTTCC 
AACACTCACA CAAACCACGA GGACAAGCTA CAGGTGAAGA ACATCATCTA CCATGCTGTG 
AAGGATGCTG TGGCAACACT AAAAGCCCAT GAGCCCAAAT TAGGCCGTTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_005173141.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173198.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X4, mRNA.

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CloneID	ODa19560
Clone ID Related Accession (Same CDS sequence)	XM_005173141.3 , XM_005173141.4
Accession Version	XM_005173141.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 3B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173141.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG CAGGAACACT CCCATATAAG GAGCACAGGT 
TTTGTGAAGG AGAATGGCAG TTTTATCCCA AACCAAGAAA GGATTTCTTC AGTATCTGCA 
GTGCTTCCTG CTTCTACTCC AACGCCACCT CCTTTGAAGC CTGCCCCATC ACCTTTTTCT 
AACACTTTTC CTTCTTTGGG TCAAATGCCC AACTTGGTGC CCGGGGCCCC TGCCCCGAAA 
TCATCTCCCA CACTCCCATC GTCAGAGAGG GAGGAGGGAG GTGTTCTTTC AGGGTATCCT 
AAAACAGCTG CCCTGGTGTC TCCAGGTCCT GTGACCATTT CATCACCTTC CCAAGAAAAC 
ACTTCAAGTG TGGCTCTCAC TGCTCCTGTT GATGCGAACC AAAAGCCCAG TATGTGGGGA 
TCAACCTCAG AAGGAAATCA GACCCCTAAA GCCCCTGTTC TAGCTCCTGC TGGATTTGGT 
AAACAGTCCA GTGGAGGGGT GTTTGGGAAT GTCTCAACAC AGACTAATGG ATCTTCCCAG 
GGGGACAAAC CCTTTGGGTT TTCATTTAGA GGTGCAAAGG ACTCGCAAAG GCAGGACTCT 
GATACGTCAC AGAACCTGTT CTTCCAATTC ATCTCTCAGA ACCAGAGCAA CACACAAGGC 
CAATCAAAAG CCTTCACATC TTTGTCCGAG TGCCTGAACA AGGAGCCGCC CAGCCTGTTC 
AAGTCTGCTG CTCCCTCTGA GGGTTTCAAA AAAACTGTTG TGGCTTCTGC ATCCACTGGA 
CTGTTTGGTT CAGCACCTGC CAGTGGCCTG GCACCACTGA AAGAGCAGCC AAAAGTGCCT 
GATATCAAGC CTGCAGGCAA TGGGATCCTT ATGAACAAGC CTTTTGGTGC TGTAGGGGAG 
GCACAGAGCA AACTCCCAGC CACTTTTTCT TCTGCCATAA TTCAAGCTGC AAGCTCTACA 
GAGGTGCTGA AACCCTCGGG ATTAGGAACC AGTGGACTTG GAAATGCCAG TGGTGGAATT 
AGAAATGTAA ATAGCTCAAC CCCAGTGAGT AGCTTTGGTC TGCTTGCTGG CAACAAGGTT 
TCCGATGGAC ACGAGAACCT GTTCTTGCAG CCCTCGAAGG AGACAAATTC GTTTCTTGCC 
TATGGCAGAG GTGTTCCGCA AACCCCTTTT GGTGCATTGA CATCTCCAAA GTCTTCATCT 
GCCCAGTCCG CATCTGCTGT TTCACCTTCA GGCAGCACCA GTCTGCTCGG TCAAGATCCT 
CCTGGTAGTG ATGCAAAACC AAATCTCTTC ACTATGGCAG AACCTCCCAA GGGAATCTTA 
GCCCCTCAGT TTGCAGCTCC TGCTCTCATG ACCACTCCGT CATTCACATC AGTTGCACAG 
GATGGACAGC AAATATCCAA ACCAAACAGA GAAGGATCAG ATGACAGCTC TGGAACCCTT 
CTCAGCACTG AAGCAGAAGG TGTCTCTGTG CCCTTTGAGC AAAGCAAGTT TTCATTGGAG 
GAACGTCTTC AGTCTATCAA AAAGGATTCA GAGTCCAGTA GTAACAGTGA CTTGTCTGAT 
TTGAGTGAGG GAGAGGACAA CGCTGGCCAG AGCCAGAAAC CCGATCTTCC GGCAGGCAAT 
GAGGATAAGA ACAAGGCCCA GGCTGCAGCC AAGAGCCGGC CACGGAGCAA ACCATTTAAA 
GTTGGACAGT CTGTGTTGAA GGACCAGAAT AAGGTACGGC GCTTGAAGCA GTCTGGAGAG 
GCCTTTCTAC AAGATGGCTC CTGCATTAAC GTGGCTCCCC ACTTGCACAA ATGTCGTGAG 
TGCCGTCTGG AGCGCTACAG GAAATCGCGT GAGCAGGACT CTGATGATGA CGACCCAAAT 
GTAGCCTGTC GATTCTTTCA CTTTCGCAGG TTGGCTTTCA CTAAAAAGGG AGTTCTTCGT 
GTGGAGGGTT TTCTGAGCCC ACAGCAGAGT GACAGCATGG CAATGGGCCT GTGGCTTCCT 
TCCATCACAA TACAAGAAGG ACTTGACCTG GACACGTCCA AATATATTTT AGCAAATGTG 
GGGGACCAGT TTTGCCAATT GGTCATGTCA GAGAAAGAAG CCATGATGAT GGTAGAACCA 
CATCAAAAAG TGGCTTGGAA GCGAGCTGTC CGAGGAGTGA GAGAAATGTG TGATGTTTGT 
GAAACAACCC TGTTCAACAT CCACTGGGTT TGTCGTAAAT GTGGCTTCGG CGTTTGTCTT 
GACTGCTATC GACTACGCAA GAACAGGCCA CCTGAAGTGG AAGATGGTCC TGAAGAGGAG 
GTGTTCTCAT GGCTAAAGTG TGCTAAAGGC CAGCCACACG AACCACAGAA CCTCATGCCT 
ACTCAAATTA TACCCGGCAC GGCATTGTAC AGCATAGGTG ACATGGTACA TGCAGCCAGA 
GGTAAATGGG GGATTAAAGC AAACTGTCCC TGCACTAGTC GGCACAAAAC GTTGATGCGG 
CCCAGTGCTC CAAATGGTCT TTCACAGTCG GGTGCAGCTC ACAGTTCGGG GAATGGAACC 
ACGTCTACTT CATCCACAAC CCGTCCAAAT GAAGAATCTG CAGCTGGTGT TGTAGTCAAA 
ACTGAACCCG TTGAAGAGCC TACAAGTGCT GATACCACAT CAAGTACCAG TGGGACAAAT 
AGCAGCACAT CTAGCCCTGC TCCTGCTCCA ACTTCTGCTC CTACTCTTCT TCCTCCTTCT 
CTTCCTCCTC CTTCACTTCC TCCTCCTTCA CTTCCTCCTG CTCCTGCACT TGTACCTACA 
CCTCCTGCAA AGGATAGCAA GAGCAGTTCT TCAGCACTTC ACTGGCTGGC AGATCTGGCC 
ACACAAAAGG AGCCCAAAGA GTCTCTGCGC TCCATGATGG GTCGTGACTC TCGTTCTCCA 
TTTGGCCTGG ACTCGTTCAG CACACTTTCC AAACCATCAG GGTCCAGTCC TAAGCTGTTC 
AACAGTCTGC TGCTGGGTGC AGGCCCTTCA CAACCCAAAG CAGAGGGAAC CAGCCTCCGA 
GATCTGCTGA ACTCTGGCTC TGGCAAACTC CCGCAAGGGC CCACAGAGGG AGGTGTCCCA 
TTTCCTCCAG TGTTCTCCAC TGCTAGTCAG GCTGATAAAA TGAAGGGAGG TCTTCCTAAC 
TTCCTGGATC ATATCATTGC ATCAGTAGTG GAGACTAAGA AGTCAGAGGT TCGTCGTGCA 
TCAGGTTCAG CAGAAGTCAT CGAGTCTGCC ACTGCTCCTC GCAGAGAGGG GGTGATGGGG 
CTGAGTGTCC TGGATCCACA CACTTCACAC TCTTGGCTTT GTGACGGCCG TTTGCTCTGC 
CTGCAGGACC CCAGTAACCC CAACAACTGG AAGATCTTCA GAGAGTGCTG GAAACAAGGA 
CAGCCTGTGC TGGTCTCAGG CATACACAGG AAGCTGAAGG AACACTTATG GAGGCCTGAG 
GCCTTCAGCG AGGAGTTTGG AGACCAGGAC GTGGACCTGG TCAACTGTAG GAACTGCGCC 
ATCATCTCTG ATGTTAAAGT CAGGGAATTT TGGGATGGCT TCCAGGTCAT TTCCAAGCGA 
TTGCAAGGTA GTGATGGCCA GCCTATGGTA CTAAAACTTA AAGACTGGCC TCCTGGAGAA 
GATTTTCGAG ATATGATGCC CACTCGGTTT AATGATCTCA TGGACAATCT TCCTTTGCCC 
GAGTACACAA AGAGAGATGG CCGTCTCAAC CTGGCCTCTC GTCTTCCAAA CTTCTTTGTT 
CGGCCAGATC TGGGCCCTAA AATGTACAAT GCGTATGGTC TGATCTCCAC AGAAGACAGG 
AAGGTCGGCA CCACTAACTT GCATTTGGAT GTGTCTGATG CCGTCAACGT TATGGTGTAT 
GTGGGCATCC CAGAAGGAGA GAATGACCAA GAAAGTGAAG CAGACCTCGC TGGATTCAAA 
GAGGTGATGC AGACTATTGA AGAGGGTGAT GTAGATGACA TGACGAAAAG AAGAGTCTAC 
GAGATCAAGG AGAAACCTGG GGCTCTCTGG CACATCTATG CTGCCAAAGA CGCTGAGAAG 
ATCCGAGAGC TCTTACGAAA GGTTGGAGAG GAACAAGGTC AAGAGAACCC ACCAGACCAT 
GATCCTATCC ATGACCAGAG CTGGTACCTG GACCAGACGT TGCGTCGCAG GCTGTATGAG 
GAGTATGGAG TCCAGGGATG GTCCATTGTG CAGTTTTTGG GAGATGCAGT GTTCATCCCA 
GCTGGAGCAC CACATCAGGT GCACAACCTG TACAGCTGTA TCAAAGCTGC AGAAGATTTT 
GTTTCTCCAG AGCATGTGAA GCACTGTTTT AGACTGACGC AAGAGTTTCG CCACCTTTCC 
AACACTCACA CAAACCACGA GGACAAGCTA CAGGTGAAGA ACATCATCTA CCATGCTGTG 
AAGGATGCTG TGGCAACACT AAAAGCCCAT GAGCCCAAAT TAGGCCGTTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173198.1
CDS	267..5600
Translation	MGDSLGLIGKRLLLLLNDGSSAPAATGGEVERAAWLRGTVRAVSVIGLASPGVEVFVEFEDSPWRKRAWVQLYGDEVRVVLMESAIVWANCSDPSLSTALGSSATQWPALMFKQLIDRVGLGSVVPVEFFGARNLAFLPNGNSLHTFETEKDFTHSLLQEQPALQHAISSWHTDSELQEILRKGSYTIQGRRVKVYQPEFEQPWAFGLVSQHDPVSHIMEITMDQSCLKGEETQVVDPRVIHVMLAEGKLNESQDRRKKEGDGGKGEGSRRRRTASEGDEDITLKRFKGAGEGASDNQNGNSSNRDAEAMQEHSHIRSTGFVKENGSFIPNQERISSVSAVLPASTPTPPPLKPAPSPFSNTFPSLGQMPNLVPGAPAPKSSPTLPSSEREEGGVLSGYPKTAALVSPGPVTISSPSQENTSSVALTAPVDANQKPSMWGSTSEGNQTPKAPVLAPAGFGKQSSGGVFGNVSTQTNGSSQGDKPFGFSFRGAKDSQRQDSDTSQNLFFQFISQNQSNTQGQSKAFTSLSECLNKEPPSLFKSAAPSEGFKKTVVASASTGLFGSAPASGLAPLKEQPKVPDIKPAGNGILMNKPFGAVGEAQSKLPATFSSAIIQAASSTEVLKPSGLGTSGLGNASGGIRNVNSSTPVSSFGLLAGNKVSDGHENLFLQPSKETNSFLAYGRGVPQTPFGALTSPKSSSAQSASAVSPSGSTSLLGQDPPGSDAKPNLFTMAEPPKGILAPQFAAPALMTTPSFTSVAQDGQQISKPNREGSDDSSGTLLSTEAEGVSVPFEQSKFSLEERLQSIKKDSESSSNSDLSDLSEGEDNAGQSQKPDLPAGNEDKNKAQAAAKSRPRSKPFKVGQSVLKDQNKVRRLKQSGEAFLQDGSCINVAPHLHKCRECRLERYRKSREQDSDDDDPNVACRFFHFRRLAFTKKGVLRVEGFLSPQQSDSMAMGLWLPSITIQEGLDLDTSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPPEVEDGPEEEVFSWLKCAKGQPHEPQNLMPTQIIPGTALYSIGDMVHAARGKWGIKANCPCTSRHKTLMRPSAPNGLSQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASQADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEADLAGFKEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_005173141.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173141.4

ORF Insert Sequence:

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG CAGGAACACT CCCATATAAG GAGCACAGGT 
TTTGTGAAGG AGAATGGCAG TTTTATCCCA AACCAAGAAA GGATTTCTTC AGTATCTGCA 
GTGCTTCCTG CTTCTACTCC AACGCCACCT CCTTTGAAGC CTGCCCCATC ACCTTTTTCT 
AACACTTTTC CTTCTTTGGG TCAAATGCCC AACTTGGTGC CCGGGGCCCC TGCCCCGAAA 
TCATCTCCCA CACTCCCATC GTCAGAGAGG GAGGAGGGAG GTGTTCTTTC AGGGTATCCT 
AAAACAGCTG CCCTGGTGTC TCCAGGTCCT GTGACCATTT CATCACCTTC CCAAGAAAAC 
ACTTCAAGTG TGGCTCTCAC TGCTCCTGTT GATGCGAACC AAAAGCCCAG TATGTGGGGA 
TCAACCTCAG AAGGAAATCA GACCCCTAAA GCCCCTGTTC TAGCTCCTGC TGGATTTGGT 
AAACAGTCCA GTGGAGGGGT GTTTGGGAAT GTCTCAACAC AGACTAATGG ATCTTCCCAG 
GGGGACAAAC CCTTTGGGTT TTCATTTAGA GGTGCAAAGG ACTCGCAAAG GCAGGACTCT 
GATACGTCAC AGAACCTGTT CTTCCAATTC ATCTCTCAGA ACCAGAGCAA CACACAAGGC 
CAATCAAAAG CCTTCACATC TTTGTCCGAG TGCCTGAACA AGGAGCCGCC CAGCCTGTTC 
AAGTCTGCTG CTCCCTCTGA GGGTTTCAAA AAAACTGTTG TGGCTTCTGC ATCCACTGGA 
CTGTTTGGTT CAGCACCTGC CAGTGGCCTG GCACCACTGA AAGAGCAGCC AAAAGTGCCT 
GATATCAAGC CTGCAGGCAA TGGGATCCTT ATGAACAAGC CTTTTGGTGC TGTAGGGGAG 
GCACAGAGCA AACTCCCAGC CACTTTTTCT TCTGCCATAA TTCAAGCTGC AAGCTCTACA 
GAGGTGCTGA AACCCTCGGG ATTAGGAACC AGTGGACTTG GAAATGCCAG TGGTGGAATT 
AGAAATGTAA ATAGCTCAAC CCCAGTGAGT AGCTTTGGTC TGCTTGCTGG CAACAAGGTT 
TCCGATGGAC ACGAGAACCT GTTCTTGCAG CCCTCGAAGG AGACAAATTC GTTTCTTGCC 
TATGGCAGAG GTGTTCCGCA AACCCCTTTT GGTGCATTGA CATCTCCAAA GTCTTCATCT 
GCCCAGTCCG CATCTGCTGT TTCACCTTCA GGCAGCACCA GTCTGCTCGG TCAAGATCCT 
CCTGGTAGTG ATGCAAAACC AAATCTCTTC ACTATGGCAG AACCTCCCAA GGGAATCTTA 
GCCCCTCAGT TTGCAGCTCC TGCTCTCATG ACCACTCCGT CATTCACATC AGTTGCACAG 
GATGGACAGC AAATATCCAA ACCAAACAGA GAAGGATCAG ATGACAGCTC TGGAACCCTT 
CTCAGCACTG AAGCAGAAGG TGTCTCTGTG CCCTTTGAGC AAAGCAAGTT TTCATTGGAG 
GAACGTCTTC AGTCTATCAA AAAGGATTCA GAGTCCAGTA GTAACAGTGA CTTGTCTGAT 
TTGAGTGAGG GAGAGGACAA CGCTGGCCAG AGCCAGAAAC CCGATCTTCC GGCAGGCAAT 
GAGGATAAGA ACAAGGCCCA GGCTGCAGCC AAGAGCCGGC CACGGAGCAA ACCATTTAAA 
GTTGGACAGT CTGTGTTGAA GGACCAGAAT AAGGTACGGC GCTTGAAGCA GTCTGGAGAG 
GCCTTTCTAC AAGATGGCTC CTGCATTAAC GTGGCTCCCC ACTTGCACAA ATGTCGTGAG 
TGCCGTCTGG AGCGCTACAG GAAATCGCGT GAGCAGGACT CTGATGATGA CGACCCAAAT 
GTAGCCTGTC GATTCTTTCA CTTTCGCAGG TTGGCTTTCA CTAAAAAGGG AGTTCTTCGT 
GTGGAGGGTT TTCTGAGCCC ACAGCAGAGT GACAGCATGG CAATGGGCCT GTGGCTTCCT 
TCCATCACAA TACAAGAAGG ACTTGACCTG GACACGTCCA AATATATTTT AGCAAATGTG 
GGGGACCAGT TTTGCCAATT GGTCATGTCA GAGAAAGAAG CCATGATGAT GGTAGAACCA 
CATCAAAAAG TGGCTTGGAA GCGAGCTGTC CGAGGAGTGA GAGAAATGTG TGATGTTTGT 
GAAACAACCC TGTTCAACAT CCACTGGGTT TGTCGTAAAT GTGGCTTCGG CGTTTGTCTT 
GACTGCTATC GACTACGCAA GAACAGGCCA CCTGAAGTGG AAGATGGTCC TGAAGAGGAG 
GTGTTCTCAT GGCTAAAGTG TGCTAAAGGC CAGCCACACG AACCACAGAA CCTCATGCCT 
ACTCAAATTA TACCCGGCAC GGCATTGTAC AGCATAGGTG ACATGGTACA TGCAGCCAGA 
GGTAAATGGG GGATTAAAGC AAACTGTCCC TGCACTAGTC GGCACAAAAC GTTGATGCGG 
CCCAGTGCTC CAAATGGTCT TTCACAGTCG GGTGCAGCTC ACAGTTCGGG GAATGGAACC 
ACGTCTACTT CATCCACAAC CCGTCCAAAT GAAGAATCTG CAGCTGGTGT TGTAGTCAAA 
ACTGAACCCG TTGAAGAGCC TACAAGTGCT GATACCACAT CAAGTACCAG TGGGACAAAT 
AGCAGCACAT CTAGCCCTGC TCCTGCTCCA ACTTCTGCTC CTACTCTTCT TCCTCCTTCT 
CTTCCTCCTC CTTCACTTCC TCCTCCTTCA CTTCCTCCTG CTCCTGCACT TGTACCTACA 
CCTCCTGCAA AGGATAGCAA GAGCAGTTCT TCAGCACTTC ACTGGCTGGC AGATCTGGCC 
ACACAAAAGG AGCCCAAAGA GTCTCTGCGC TCCATGATGG GTCGTGACTC TCGTTCTCCA 
TTTGGCCTGG ACTCGTTCAG CACACTTTCC AAACCATCAG GGTCCAGTCC TAAGCTGTTC 
AACAGTCTGC TGCTGGGTGC AGGCCCTTCA CAACCCAAAG CAGAGGGAAC CAGCCTCCGA 
GATCTGCTGA ACTCTGGCTC TGGCAAACTC CCGCAAGGGC CCACAGAGGG AGGTGTCCCA 
TTTCCTCCAG TGTTCTCCAC TGCTAGTCAG GCTGATAAAA TGAAGGGAGG TCTTCCTAAC 
TTCCTGGATC ATATCATTGC ATCAGTAGTG GAGACTAAGA AGTCAGAGGT TCGTCGTGCA 
TCAGGTTCAG CAGAAGTCAT CGAGTCTGCC ACTGCTCCTC GCAGAGAGGG GGTGATGGGG 
CTGAGTGTCC TGGATCCACA CACTTCACAC TCTTGGCTTT GTGACGGCCG TTTGCTCTGC 
CTGCAGGACC CCAGTAACCC CAACAACTGG AAGATCTTCA GAGAGTGCTG GAAACAAGGA 
CAGCCTGTGC TGGTCTCAGG CATACACAGG AAGCTGAAGG AACACTTATG GAGGCCTGAG 
GCCTTCAGCG AGGAGTTTGG AGACCAGGAC GTGGACCTGG TCAACTGTAG GAACTGCGCC 
ATCATCTCTG ATGTTAAAGT CAGGGAATTT TGGGATGGCT TCCAGGTCAT TTCCAAGCGA 
TTGCAAGGTA GTGATGGCCA GCCTATGGTA CTAAAACTTA AAGACTGGCC TCCTGGAGAA 
GATTTTCGAG ATATGATGCC CACTCGGTTT AATGATCTCA TGGACAATCT TCCTTTGCCC 
GAGTACACAA AGAGAGATGG CCGTCTCAAC CTGGCCTCTC GTCTTCCAAA CTTCTTTGTT 
CGGCCAGATC TGGGCCCTAA AATGTACAAT GCGTATGGTC TGATCTCCAC AGAAGACAGG 
AAGGTCGGCA CCACTAACTT GCATTTGGAT GTGTCTGATG CCGTCAACGT TATGGTGTAT 
GTGGGCATCC CAGAAGGAGA GAATGACCAA GAAAGTGAAG CAGACCTCGC TGGATTCAAA 
GAGGTGATGC AGACTATTGA AGAGGGTGAT GTAGATGACA TGACGAAAAG AAGAGTCTAC 
GAGATCAAGG AGAAACCTGG GGCTCTCTGG CACATCTATG CTGCCAAAGA CGCTGAGAAG 
ATCCGAGAGC TCTTACGAAA GGTTGGAGAG GAACAAGGTC AAGAGAACCC ACCAGACCAT 
GATCCTATCC ATGACCAGAG CTGGTACCTG GACCAGACGT TGCGTCGCAG GCTGTATGAG 
GAGTATGGAG TCCAGGGATG GTCCATTGTG CAGTTTTTGG GAGATGCAGT GTTCATCCCA 
GCTGGAGCAC CACATCAGGT GCACAACCTG TACAGCTGTA TCAAAGCTGC AGAAGATTTT 
GTTTCTCCAG AGCATGTGAA GCACTGTTTT AGACTGACGC AAGAGTTTCG CCACCTTTCC 
AACACTCACA CAAACCACGA GGACAAGCTA CAGGTGAAGA ACATCATCTA CCATGCTGTG 
AAGGATGCTG TGGCAACACT AAAAGCCCAT GAGCCCAAAT TAGGCCGTTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_005173142.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173199.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X5, mRNA.

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CloneID	ODa19561
Clone ID Related Accession (Same CDS sequence)	XM_005173142.3 , XM_005173142.4
Accession Version	XM_005173142.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 3B isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173142.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GAACACTCCC ATATAAGGAG CACAGGTTTT 
GTGAAGGAGA ATGGCAGTTT TATCCCAAAC CAAGAAAGGA TTTCTTCAGT ATCTGCAGTG 
CTTCCTGCTT CTACTCCAAC GCCACCTCCT TTGAAGCCTG CCCCATCACC TTTTTCTAAC 
ACTTTTCCTT CTTTGGGTCA AATGCCCAAC TTGGTGCCCG GGGCCCCTGC CCCGAAATCA 
TCTCCCACAC TCCCATCGTC AGAGAGGGAG GAGGGAGGTG TTCTTTCAGG GTATCCTAAA 
ACAGCTGCCC TGGTGTCTCC AGGTCCTGTG ACCATTTCAT CACCTTCCCA AGAAAACACT 
TCAAGTGTGG CTCTCACTGC TCCTGTTGAT GCGAACCAAA AGCCCAGTAT GTGGGGATCA 
ACCTCAGAAG GAAATCAGAC CCCTAAAGCC CCTGTTCTAG CTCCTGCTGG ATTTGGTAAA 
CAGTCCAGTG GAGGGGTGTT TGGGAATGTC TCAACACAGA CTAATGGATC TTCCCAGGGG 
GACAAACCCT TTGGGTTTTC ATTTAGAGGT GCAAAGGACT CGCAAAGGCA GGACTCTGAT 
ACGTCACAGA ACCTGTTCTT CCAATTCATC TCTCAGAACC AGAGCAACAC ACAAGGCCAA 
TCAAAAGCCT TCACATCTTT GTCCGAGTGC CTGAACAAGG AGCCGCCCAG CCTGTTCAAG 
TCTGCTGCTC CCTCTGAGGG TTTCAAAAAA ACTGTTGTGG CTTCTGCATC CACTGGACTG 
TTTGGTTCAG CACCTGCCAG TGGCCTGGCA CCACTGAAAG AGCAGCCAAA AGTGCCTGAT 
ATCAAGCCTG CAGGCAATGG GATCCTTATG AACAAGCCTT TTGGTGCTGT AGGGGAGGCA 
CAGAGCAAAC TCCCAGCCAC TTTTTCTTCT GCCATAATTC AAGCTGCAAG CTCTACAGAG 
GTGCTGAAAC CCTCGGGATT AGGAACCAGT GGACTTGGAA ATGCCAGTGG TGGAATTAGA 
AATGTAAATA GCTCAACCCC AGTGAGTAGC TTTGGTCTGC TTGCTGGCAA CAAGGTTTCC 
GATGGACACG AGAACCTGTT CTTGCAGCCC TCGAAGGAGA CAAATTCGTT TCTTGCCTAT 
GGCAGAGGTG TTCCGCAAAC CCCTTTTGGT GCATTGACAT CTCCAAAGTC TTCATCTGCC 
CAGTCCGCAT CTGCTGTTTC ACCTTCAGGC AGCACCAGTC TGCTCGGTCA AGATCCTCCT 
GGTAGTGATG CAAAACCAAA TCTCTTCACT ATGGCAGAAC CTCCCAAGGG AATCTTAGCC 
CCTCAGTTTG CAGCTCCTGC TCTCATGACC ACTCCGTCAT TCACATCAGT TGCACAGGAT 
GGACAGCAAA TATCCAAACC AAACAGAGAA GGATCAGATG ACAGCTCTGG AACCCTTCTC 
AGCACTGAAG CAGAAGGTGT CTCTGTGCCC TTTGAGCAAA GCAAGTTTTC ATTGGAGGAA 
CGTCTTCAGT CTATCAAAAA GGATTCAGAG TCCAGTAGTA ACAGTGACTT GTCTGATTTG 
AGTGAGGGAG AGGACAACGC TGGCCAGAGC CAGAAACCCG ATCTTCCGGC AGGCAATGAG 
GATAAGAACA AGGCCCAGGC TGCAGCCAAG AGCCGGCCAC GGAGCAAACC ATTTAAAGTT 
GGACAGTCTG TGTTGAAGGA CCAGAATAAG GTACGGCGCT TGAAGCAGTC TGGAGAGGCC 
TTTCTACAAG ATGGCTCCTG CATTAACGTG GCTCCCCACT TGCACAAATG TCGTGAGTGC 
CGTCTGGAGC GCTACAGGAA ATCGCGTGAG CAGGACTCTG ATGATGACGA CCCAAATGTA 
GCCTGTCGAT TCTTTCACTT TCGCAGGTTG GCTTTCACTA AAAAGGGAGT TCTTCGTGTG 
GAGGGTTTTC TGAGCCCACA GCAGAGTGAC AGCATGGCAA TGGGCCTGTG GCTTCCTTCC 
ATCACAATAC AAGAAGGACT TGACCTGGAC ACGTCCAAAT ATATTTTAGC AAATGTGGGG 
GACCAGTTTT GCCAATTGGT CATGTCAGAG AAAGAAGCCA TGATGATGGT AGAACCACAT 
CAAAAAGTGG CTTGGAAGCG AGCTGTCCGA GGAGTGAGAG AAATGTGTGA TGTTTGTGAA 
ACAACCCTGT TCAACATCCA CTGGGTTTGT CGTAAATGTG GCTTCGGCGT TTGTCTTGAC 
TGCTATCGAC TACGCAAGAA CAGGCCACCT GAAGTGGAAG ATGGTCCTGA AGAGGAGGTG 
TTCTCATGGC TAAAGTGTGC TAAAGGCCAG CCACACGAAC CACAGAACCT CATGCCTACT 
CAAATTATAC CCGGCACGGC ATTGTACAGC ATAGGTGACA TGGTACATGC AGCCAGAGGT 
AAATGGGGGA TTAAAGCAAA CTGTCCCTGC ACTAGTCGGC ACAAAACGTT GATGCGGCCC 
AGTGCTCCAA ATGGTCTTTC ACAGTCGGGT GCAGCTCACA GTTCGGGGAA TGGAACCACG 
TCTACTTCAT CCACAACCCG TCCAAATGAA GAATCTGCAG CTGGTGTTGT AGTCAAAACT 
GAACCCGTTG AAGAGCCTAC AAGTGCTGAT ACCACATCAA GTACCAGTGG GACAAATAGC 
AGCACATCTA GCCCTGCTCC TGCTCCAACT TCTGCTCCTA CTCTTCTTCC TCCTTCTCTT 
CCTCCTCCTT CACTTCCTCC TCCTTCACTT CCTCCTGCTC CTGCACTTGT ACCTACACCT 
CCTGCAAAGG ATAGCAAGAG CAGTTCTTCA GCACTTCACT GGCTGGCAGA TCTGGCCACA 
CAAAAGGAGC CCAAAGAGTC TCTGCGCTCC ATGATGGGTC GTGACTCTCG TTCTCCATTT 
GGCCTGGACT CGTTCAGCAC ACTTTCCAAA CCATCAGGGT CCAGTCCTAA GCTGTTCAAC 
AGTCTGCTGC TGGGTGCAGG CCCTTCACAA CCCAAAGCAG AGGGAACCAG CCTCCGAGAT 
CTGCTGAACT CTGGCTCTGG CAAACTCCCG CAAGGGCCCA CAGAGGGAGG TGTCCCATTT 
CCTCCAGTGT TCTCCACTGC TAGTCAGGCT GATAAAATGA AGGGAGGTCT TCCTAACTTC 
CTGGATCATA TCATTGCATC AGTAGTGGAG ACTAAGAAGT CAGAGGTTCG TCGTGCATCA 
GGTTCAGCAG AAGTCATCGA GTCTGCCACT GCTCCTCGCA GAGAGGGGGT GATGGGGCTG 
AGTGTCCTGG ATCCACACAC TTCACACTCT TGGCTTTGTG ACGGCCGTTT GCTCTGCCTG 
CAGGACCCCA GTAACCCCAA CAACTGGAAG ATCTTCAGAG AGTGCTGGAA ACAAGGACAG 
CCTGTGCTGG TCTCAGGCAT ACACAGGAAG CTGAAGGAAC ACTTATGGAG GCCTGAGGCC 
TTCAGCGAGG AGTTTGGAGA CCAGGACGTG GACCTGGTCA ACTGTAGGAA CTGCGCCATC 
ATCTCTGATG TTAAAGTCAG GGAATTTTGG GATGGCTTCC AGGTCATTTC CAAGCGATTG 
CAAGGTAGTG ATGGCCAGCC TATGGTACTA AAACTTAAAG ACTGGCCTCC TGGAGAAGAT 
TTTCGAGATA TGATGCCCAC TCGGTTTAAT GATCTCATGG ACAATCTTCC TTTGCCCGAG 
TACACAAAGA GAGATGGCCG TCTCAACCTG GCCTCTCGTC TTCCAAACTT CTTTGTTCGG 
CCAGATCTGG GCCCTAAAAT GTACAATGCG TATGGTCTGA TCTCCACAGA AGACAGGAAG 
GTCGGCACCA CTAACTTGCA TTTGGATGTG TCTGATGCCG TCAACGTTAT GGTGTATGTG 
GGCATCCCAG AAGGAGAGAA TGACCAAGAA AGTGAAGCAG ACCTCGCTGG ATTCAAAGAG 
GTGATGCAGA CTATTGAAGA GGGTGATGTA GATGACATGA CGAAAAGAAG AGTCTACGAG 
ATCAAGGAGA AACCTGGGGC TCTCTGGCAC ATCTATGCTG CCAAAGACGC TGAGAAGATC 
CGAGAGCTCT TACGAAAGGT TGGAGAGGAA CAAGGTCAAG AGAACCCACC AGACCATGAT 
CCTATCCATG ACCAGAGCTG GTACCTGGAC CAGACGTTGC GTCGCAGGCT GTATGAGGAG 
TATGGAGTCC AGGGATGGTC CATTGTGCAG TTTTTGGGAG ATGCAGTGTT CATCCCAGCT 
GGAGCACCAC ATCAGGTGCA CAACCTGTAC AGCTGTATCA AAGCTGCAGA AGATTTTGTT 
TCTCCAGAGC ATGTGAAGCA CTGTTTTAGA CTGACGCAAG AGTTTCGCCA CCTTTCCAAC 
ACTCACACAA ACCACGAGGA CAAGCTACAG GTGAAGAACA TCATCTACCA TGCTGTGAAG 
GATGCTGTGG CAACACTAAA AGCCCATGAG CCCAAATTAG GCCGTTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173199.1
CDS	266..5596
Translation	MGDSLGLIGKRLLLLLNDGSSAPAATGGEVERAAWLRGTVRAVSVIGLASPGVEVFVEFEDSPWRKRAWVQLYGDEVRVVLMESAIVWANCSDPSLSTALGSSATQWPALMFKQLIDRVGLGSVVPVEFFGARNLAFLPNGNSLHTFETEKDFTHSLLQEQPALQHAISSWHTDSELQEILRKGSYTIQGRRVKVYQPEFEQPWAFGLVSQHDPVSHIMEITMDQSCLKGEETQVVDPRVIHVMLAEGKLNESQDRRKKEGDGGKGEGSRRRRTASEGDEDITLKRFKGAGEGASDNQNGNSSNRDAEAMEHSHIRSTGFVKENGSFIPNQERISSVSAVLPASTPTPPPLKPAPSPFSNTFPSLGQMPNLVPGAPAPKSSPTLPSSEREEGGVLSGYPKTAALVSPGPVTISSPSQENTSSVALTAPVDANQKPSMWGSTSEGNQTPKAPVLAPAGFGKQSSGGVFGNVSTQTNGSSQGDKPFGFSFRGAKDSQRQDSDTSQNLFFQFISQNQSNTQGQSKAFTSLSECLNKEPPSLFKSAAPSEGFKKTVVASASTGLFGSAPASGLAPLKEQPKVPDIKPAGNGILMNKPFGAVGEAQSKLPATFSSAIIQAASSTEVLKPSGLGTSGLGNASGGIRNVNSSTPVSSFGLLAGNKVSDGHENLFLQPSKETNSFLAYGRGVPQTPFGALTSPKSSSAQSASAVSPSGSTSLLGQDPPGSDAKPNLFTMAEPPKGILAPQFAAPALMTTPSFTSVAQDGQQISKPNREGSDDSSGTLLSTEAEGVSVPFEQSKFSLEERLQSIKKDSESSSNSDLSDLSEGEDNAGQSQKPDLPAGNEDKNKAQAAAKSRPRSKPFKVGQSVLKDQNKVRRLKQSGEAFLQDGSCINVAPHLHKCRECRLERYRKSREQDSDDDDPNVACRFFHFRRLAFTKKGVLRVEGFLSPQQSDSMAMGLWLPSITIQEGLDLDTSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPPEVEDGPEEEVFSWLKCAKGQPHEPQNLMPTQIIPGTALYSIGDMVHAARGKWGIKANCPCTSRHKTLMRPSAPNGLSQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASQADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEADLAGFKEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_005173142.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173142.3

ORF Insert Sequence:

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GAACACTCCC ATATAAGGAG CACAGGTTTT 
GTGAAGGAGA ATGGCAGTTT TATCCCAAAC CAAGAAAGGA TTTCTTCAGT ATCTGCAGTG 
CTTCCTGCTT CTACTCCAAC GCCACCTCCT TTGAAGCCTG CCCCATCACC TTTTTCTAAC 
ACTTTTCCTT CTTTGGGTCA AATGCCCAAC TTGGTGCCCG GGGCCCCTGC CCCGAAATCA 
TCTCCCACAC TCCCATCGTC AGAGAGGGAG GAGGGAGGTG TTCTTTCAGG GTATCCTAAA 
ACAGCTGCCC TGGTGTCTCC AGGTCCTGTG ACCATTTCAT CACCTTCCCA AGAAAACACT 
TCAAGTGTGG CTCTCACTGC TCCTGTTGAT GCGAACCAAA AGCCCAGTAT GTGGGGATCA 
ACCTCAGAAG GAAATCAGAC CCCTAAAGCC CCTGTTCTAG CTCCTGCTGG ATTTGGTAAA 
CAGTCCAGTG GAGGGGTGTT TGGGAATGTC TCAACACAGA CTAATGGATC TTCCCAGGGG 
GACAAACCCT TTGGGTTTTC ATTTAGAGGT GCAAAGGACT CGCAAAGGCA GGACTCTGAT 
ACGTCACAGA ACCTGTTCTT CCAATTCATC TCTCAGAACC AGAGCAACAC ACAAGGCCAA 
TCAAAAGCCT TCACATCTTT GTCCGAGTGC CTGAACAAGG AGCCGCCCAG CCTGTTCAAG 
TCTGCTGCTC CCTCTGAGGG TTTCAAAAAA ACTGTTGTGG CTTCTGCATC CACTGGACTG 
TTTGGTTCAG CACCTGCCAG TGGCCTGGCA CCACTGAAAG AGCAGCCAAA AGTGCCTGAT 
ATCAAGCCTG CAGGCAATGG GATCCTTATG AACAAGCCTT TTGGTGCTGT AGGGGAGGCA 
CAGAGCAAAC TCCCAGCCAC TTTTTCTTCT GCCATAATTC AAGCTGCAAG CTCTACAGAG 
GTGCTGAAAC CCTCGGGATT AGGAACCAGT GGACTTGGAA ATGCCAGTGG TGGAATTAGA 
AATGTAAATA GCTCAACCCC AGTGAGTAGC TTTGGTCTGC TTGCTGGCAA CAAGGTTTCC 
GATGGACACG AGAACCTGTT CTTGCAGCCC TCGAAGGAGA CAAATTCGTT TCTTGCCTAT 
GGCAGAGGTG TTCCGCAAAC CCCTTTTGGT GCATTGACAT CTCCAAAGTC TTCATCTGCC 
CAGTCCGCAT CTGCTGTTTC ACCTTCAGGC AGCACCAGTC TGCTCGGTCA AGATCCTCCT 
GGTAGTGATG CAAAACCAAA TCTCTTCACT ATGGCAGAAC CTCCCAAGGG AATCTTAGCC 
CCTCAGTTTG CAGCTCCTGC TCTCATGACC ACTCCGTCAT TCACATCAGT TGCACAGGAT 
GGACAGCAAA TATCCAAACC AAACAGAGAA GGATCAGATG ACAGCTCTGG AACCCTTCTC 
AGCACTGAAG CAGAAGGTGT CTCTGTGCCC TTTGAGCAAA GCAAGTTTTC ATTGGAGGAA 
CGTCTTCAGT CTATCAAAAA GGATTCAGAG TCCAGTAGTA ACAGTGACTT GTCTGATTTG 
AGTGAGGGAG AGGACAACGC TGGCCAGAGC CAGAAACCCG ATCTTCCGGC AGGCAATGAG 
GATAAGAACA AGGCCCAGGC TGCAGCCAAG AGCCGGCCAC GGAGCAAACC ATTTAAAGTT 
GGACAGTCTG TGTTGAAGGA CCAGAATAAG GTACGGCGCT TGAAGCAGTC TGGAGAGGCC 
TTTCTACAAG ATGGCTCCTG CATTAACGTG GCTCCCCACT TGCACAAATG TCGTGAGTGC 
CGTCTGGAGC GCTACAGGAA ATCGCGTGAG CAGGACTCTG ATGATGACGA CCCAAATGTA 
GCCTGTCGAT TCTTTCACTT TCGCAGGTTG GCTTTCACTA AAAAGGGAGT TCTTCGTGTG 
GAGGGTTTTC TGAGCCCACA GCAGAGTGAC AGCATGGCAA TGGGCCTGTG GCTTCCTTCC 
ATCACAATAC AAGAAGGACT TGACCTGGAC ACGTCCAAAT ATATTTTAGC AAATGTGGGG 
GACCAGTTTT GCCAATTGGT CATGTCAGAG AAAGAAGCCA TGATGATGGT AGAACCACAT 
CAAAAAGTGG CTTGGAAGCG AGCTGTCCGA GGAGTGAGAG AAATGTGTGA TGTTTGTGAA 
ACAACCCTGT TCAACATCCA CTGGGTTTGT CGTAAATGTG GCTTCGGCGT TTGTCTTGAC 
TGCTATCGAC TACGCAAGAA CAGGCCACCT GAAGTGGAAG ATGGTCCTGA AGAGGAGGTG 
TTCTCATGGC TAAAGTGTGC TAAAGGCCAG CCACACGAAC CACAGAACCT CATGCCTACT 
CAAATTATAC CCGGCACGGC ATTGTACAGC ATAGGTGACA TGGTACATGC AGCCAGAGGT 
AAATGGGGGA TTAAAGCAAA CTGTCCCTGC ACTAGTCGGC ACAAAACGTT GATGCGGCCC 
AGTGCTCCAA ATGGTCTTTC ACAGTCGGGT GCAGCTCACA GTTCGGGGAA TGGAACCACG 
TCTACTTCAT CCACAACCCG TCCAAATGAA GAATCTGCAG CTGGTGTTGT AGTCAAAACT 
GAACCCGTTG AAGAGCCTAC AAGTGCTGAT ACCACATCAA GTACCAGTGG GACAAATAGC 
AGCACATCTA GCCCTGCTCC TGCTCCAACT TCTGCTCCTA CTCTTCTTCC TCCTTCTCTT 
CCTCCTCCTT CACTTCCTCC TCCTTCACTT CCTCCTGCTC CTGCACTTGT ACCTACACCT 
CCTGCAAAGG ATAGCAAGAG CAGTTCTTCA GCACTTCACT GGCTGGCAGA TCTGGCCACA 
CAAAAGGAGC CCAAAGAGTC TCTGCGCTCC ATGATGGGTC GTGACTCTCG TTCTCCATTT 
GGCCTGGACT CGTTCAGCAC ACTTTCCAAA CCATCAGGGT CCAGTCCTAA GCTGTTCAAC 
AGTCTGCTGC TGGGTGCAGG CCCTTCACAA CCCAAAGCAG AGGGAACCAG CCTCCGAGAT 
CTGCTGAACT CTGGCTCTGG CAAACTCCCG CAAGGGCCCA CAGAGGGAGG TGTCCCATTT 
CCTCCAGTGT TCTCCACTGC TAGTCAGGCT GATAAAATGA AGGGAGGTCT TCCTAACTTC 
CTGGATCATA TCATTGCATC AGTAGTGGAG ACTAAGAAGT CAGAGGTTCG TCGTGCATCA 
GGTTCAGCAG AAGTCATCGA GTCTGCCACT GCTCCTCGCA GAGAGGGGGT GATGGGGCTG 
AGTGTCCTGG ATCCACACAC TTCACACTCT TGGCTTTGTG ACGGCCGTTT GCTCTGCCTG 
CAGGACCCCA GTAACCCCAA CAACTGGAAG ATCTTCAGAG AGTGCTGGAA ACAAGGACAG 
CCTGTGCTGG TCTCAGGCAT ACACAGGAAG CTGAAGGAAC ACTTATGGAG GCCTGAGGCC 
TTCAGCGAGG AGTTTGGAGA CCAGGACGTG GACCTGGTCA ACTGTAGGAA CTGCGCCATC 
ATCTCTGATG TTAAAGTCAG GGAATTTTGG GATGGCTTCC AGGTCATTTC CAAGCGATTG 
CAAGGTAGTG ATGGCCAGCC TATGGTACTA AAACTTAAAG ACTGGCCTCC TGGAGAAGAT 
TTTCGAGATA TGATGCCCAC TCGGTTTAAT GATCTCATGG ACAATCTTCC TTTGCCCGAG 
TACACAAAGA GAGATGGCCG TCTCAACCTG GCCTCTCGTC TTCCAAACTT CTTTGTTCGG 
CCAGATCTGG GCCCTAAAAT GTACAATGCG TATGGTCTGA TCTCCACAGA AGACAGGAAG 
GTCGGCACCA CTAACTTGCA TTTGGATGTG TCTGATGCCG TCAACGTTAT GGTGTATGTG 
GGCATCCCAG AAGGAGAGAA TGACCAAGAA AGTGAAGCAG ACCTCGCTGG ATTCAAAGAG 
GTGATGCAGA CTATTGAAGA GGGTGATGTA GATGACATGA CGAAAAGAAG AGTCTACGAG 
ATCAAGGAGA AACCTGGGGC TCTCTGGCAC ATCTATGCTG CCAAAGACGC TGAGAAGATC 
CGAGAGCTCT TACGAAAGGT TGGAGAGGAA CAAGGTCAAG AGAACCCACC AGACCATGAT 
CCTATCCATG ACCAGAGCTG GTACCTGGAC CAGACGTTGC GTCGCAGGCT GTATGAGGAG 
TATGGAGTCC AGGGATGGTC CATTGTGCAG TTTTTGGGAG ATGCAGTGTT CATCCCAGCT 
GGAGCACCAC ATCAGGTGCA CAACCTGTAC AGCTGTATCA AAGCTGCAGA AGATTTTGTT 
TCTCCAGAGC ATGTGAAGCA CTGTTTTAGA CTGACGCAAG AGTTTCGCCA CCTTTCCAAC 
ACTCACACAA ACCACGAGGA CAAGCTACAG GTGAAGAACA TCATCTACCA TGCTGTGAAG 
GATGCTGTGG CAACACTAAA AGCCCATGAG CCCAAATTAG GCCGTTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm3b ( XM_005173142.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173199.1 Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X5, mRNA.

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CloneID	ODa19561
Clone ID Related Accession (Same CDS sequence)	XM_005173142.3 , XM_005173142.4
Accession Version	XM_005173142.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 3B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173142.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GAACACTCCC ATATAAGGAG CACAGGTTTT 
GTGAAGGAGA ATGGCAGTTT TATCCCAAAC CAAGAAAGGA TTTCTTCAGT ATCTGCAGTG 
CTTCCTGCTT CTACTCCAAC GCCACCTCCT TTGAAGCCTG CCCCATCACC TTTTTCTAAC 
ACTTTTCCTT CTTTGGGTCA AATGCCCAAC TTGGTGCCCG GGGCCCCTGC CCCGAAATCA 
TCTCCCACAC TCCCATCGTC AGAGAGGGAG GAGGGAGGTG TTCTTTCAGG GTATCCTAAA 
ACAGCTGCCC TGGTGTCTCC AGGTCCTGTG ACCATTTCAT CACCTTCCCA AGAAAACACT 
TCAAGTGTGG CTCTCACTGC TCCTGTTGAT GCGAACCAAA AGCCCAGTAT GTGGGGATCA 
ACCTCAGAAG GAAATCAGAC CCCTAAAGCC CCTGTTCTAG CTCCTGCTGG ATTTGGTAAA 
CAGTCCAGTG GAGGGGTGTT TGGGAATGTC TCAACACAGA CTAATGGATC TTCCCAGGGG 
GACAAACCCT TTGGGTTTTC ATTTAGAGGT GCAAAGGACT CGCAAAGGCA GGACTCTGAT 
ACGTCACAGA ACCTGTTCTT CCAATTCATC TCTCAGAACC AGAGCAACAC ACAAGGCCAA 
TCAAAAGCCT TCACATCTTT GTCCGAGTGC CTGAACAAGG AGCCGCCCAG CCTGTTCAAG 
TCTGCTGCTC CCTCTGAGGG TTTCAAAAAA ACTGTTGTGG CTTCTGCATC CACTGGACTG 
TTTGGTTCAG CACCTGCCAG TGGCCTGGCA CCACTGAAAG AGCAGCCAAA AGTGCCTGAT 
ATCAAGCCTG CAGGCAATGG GATCCTTATG AACAAGCCTT TTGGTGCTGT AGGGGAGGCA 
CAGAGCAAAC TCCCAGCCAC TTTTTCTTCT GCCATAATTC AAGCTGCAAG CTCTACAGAG 
GTGCTGAAAC CCTCGGGATT AGGAACCAGT GGACTTGGAA ATGCCAGTGG TGGAATTAGA 
AATGTAAATA GCTCAACCCC AGTGAGTAGC TTTGGTCTGC TTGCTGGCAA CAAGGTTTCC 
GATGGACACG AGAACCTGTT CTTGCAGCCC TCGAAGGAGA CAAATTCGTT TCTTGCCTAT 
GGCAGAGGTG TTCCGCAAAC CCCTTTTGGT GCATTGACAT CTCCAAAGTC TTCATCTGCC 
CAGTCCGCAT CTGCTGTTTC ACCTTCAGGC AGCACCAGTC TGCTCGGTCA AGATCCTCCT 
GGTAGTGATG CAAAACCAAA TCTCTTCACT ATGGCAGAAC CTCCCAAGGG AATCTTAGCC 
CCTCAGTTTG CAGCTCCTGC TCTCATGACC ACTCCGTCAT TCACATCAGT TGCACAGGAT 
GGACAGCAAA TATCCAAACC AAACAGAGAA GGATCAGATG ACAGCTCTGG AACCCTTCTC 
AGCACTGAAG CAGAAGGTGT CTCTGTGCCC TTTGAGCAAA GCAAGTTTTC ATTGGAGGAA 
CGTCTTCAGT CTATCAAAAA GGATTCAGAG TCCAGTAGTA ACAGTGACTT GTCTGATTTG 
AGTGAGGGAG AGGACAACGC TGGCCAGAGC CAGAAACCCG ATCTTCCGGC AGGCAATGAG 
GATAAGAACA AGGCCCAGGC TGCAGCCAAG AGCCGGCCAC GGAGCAAACC ATTTAAAGTT 
GGACAGTCTG TGTTGAAGGA CCAGAATAAG GTACGGCGCT TGAAGCAGTC TGGAGAGGCC 
TTTCTACAAG ATGGCTCCTG CATTAACGTG GCTCCCCACT TGCACAAATG TCGTGAGTGC 
CGTCTGGAGC GCTACAGGAA ATCGCGTGAG CAGGACTCTG ATGATGACGA CCCAAATGTA 
GCCTGTCGAT TCTTTCACTT TCGCAGGTTG GCTTTCACTA AAAAGGGAGT TCTTCGTGTG 
GAGGGTTTTC TGAGCCCACA GCAGAGTGAC AGCATGGCAA TGGGCCTGTG GCTTCCTTCC 
ATCACAATAC AAGAAGGACT TGACCTGGAC ACGTCCAAAT ATATTTTAGC AAATGTGGGG 
GACCAGTTTT GCCAATTGGT CATGTCAGAG AAAGAAGCCA TGATGATGGT AGAACCACAT 
CAAAAAGTGG CTTGGAAGCG AGCTGTCCGA GGAGTGAGAG AAATGTGTGA TGTTTGTGAA 
ACAACCCTGT TCAACATCCA CTGGGTTTGT CGTAAATGTG GCTTCGGCGT TTGTCTTGAC 
TGCTATCGAC TACGCAAGAA CAGGCCACCT GAAGTGGAAG ATGGTCCTGA AGAGGAGGTG 
TTCTCATGGC TAAAGTGTGC TAAAGGCCAG CCACACGAAC CACAGAACCT CATGCCTACT 
CAAATTATAC CCGGCACGGC ATTGTACAGC ATAGGTGACA TGGTACATGC AGCCAGAGGT 
AAATGGGGGA TTAAAGCAAA CTGTCCCTGC ACTAGTCGGC ACAAAACGTT GATGCGGCCC 
AGTGCTCCAA ATGGTCTTTC ACAGTCGGGT GCAGCTCACA GTTCGGGGAA TGGAACCACG 
TCTACTTCAT CCACAACCCG TCCAAATGAA GAATCTGCAG CTGGTGTTGT AGTCAAAACT 
GAACCCGTTG AAGAGCCTAC AAGTGCTGAT ACCACATCAA GTACCAGTGG GACAAATAGC 
AGCACATCTA GCCCTGCTCC TGCTCCAACT TCTGCTCCTA CTCTTCTTCC TCCTTCTCTT 
CCTCCTCCTT CACTTCCTCC TCCTTCACTT CCTCCTGCTC CTGCACTTGT ACCTACACCT 
CCTGCAAAGG ATAGCAAGAG CAGTTCTTCA GCACTTCACT GGCTGGCAGA TCTGGCCACA 
CAAAAGGAGC CCAAAGAGTC TCTGCGCTCC ATGATGGGTC GTGACTCTCG TTCTCCATTT 
GGCCTGGACT CGTTCAGCAC ACTTTCCAAA CCATCAGGGT CCAGTCCTAA GCTGTTCAAC 
AGTCTGCTGC TGGGTGCAGG CCCTTCACAA CCCAAAGCAG AGGGAACCAG CCTCCGAGAT 
CTGCTGAACT CTGGCTCTGG CAAACTCCCG CAAGGGCCCA CAGAGGGAGG TGTCCCATTT 
CCTCCAGTGT TCTCCACTGC TAGTCAGGCT GATAAAATGA AGGGAGGTCT TCCTAACTTC 
CTGGATCATA TCATTGCATC AGTAGTGGAG ACTAAGAAGT CAGAGGTTCG TCGTGCATCA 
GGTTCAGCAG AAGTCATCGA GTCTGCCACT GCTCCTCGCA GAGAGGGGGT GATGGGGCTG 
AGTGTCCTGG ATCCACACAC TTCACACTCT TGGCTTTGTG ACGGCCGTTT GCTCTGCCTG 
CAGGACCCCA GTAACCCCAA CAACTGGAAG ATCTTCAGAG AGTGCTGGAA ACAAGGACAG 
CCTGTGCTGG TCTCAGGCAT ACACAGGAAG CTGAAGGAAC ACTTATGGAG GCCTGAGGCC 
TTCAGCGAGG AGTTTGGAGA CCAGGACGTG GACCTGGTCA ACTGTAGGAA CTGCGCCATC 
ATCTCTGATG TTAAAGTCAG GGAATTTTGG GATGGCTTCC AGGTCATTTC CAAGCGATTG 
CAAGGTAGTG ATGGCCAGCC TATGGTACTA AAACTTAAAG ACTGGCCTCC TGGAGAAGAT 
TTTCGAGATA TGATGCCCAC TCGGTTTAAT GATCTCATGG ACAATCTTCC TTTGCCCGAG 
TACACAAAGA GAGATGGCCG TCTCAACCTG GCCTCTCGTC TTCCAAACTT CTTTGTTCGG 
CCAGATCTGG GCCCTAAAAT GTACAATGCG TATGGTCTGA TCTCCACAGA AGACAGGAAG 
GTCGGCACCA CTAACTTGCA TTTGGATGTG TCTGATGCCG TCAACGTTAT GGTGTATGTG 
GGCATCCCAG AAGGAGAGAA TGACCAAGAA AGTGAAGCAG ACCTCGCTGG ATTCAAAGAG 
GTGATGCAGA CTATTGAAGA GGGTGATGTA GATGACATGA CGAAAAGAAG AGTCTACGAG 
ATCAAGGAGA AACCTGGGGC TCTCTGGCAC ATCTATGCTG CCAAAGACGC TGAGAAGATC 
CGAGAGCTCT TACGAAAGGT TGGAGAGGAA CAAGGTCAAG AGAACCCACC AGACCATGAT 
CCTATCCATG ACCAGAGCTG GTACCTGGAC CAGACGTTGC GTCGCAGGCT GTATGAGGAG 
TATGGAGTCC AGGGATGGTC CATTGTGCAG TTTTTGGGAG ATGCAGTGTT CATCCCAGCT 
GGAGCACCAC ATCAGGTGCA CAACCTGTAC AGCTGTATCA AAGCTGCAGA AGATTTTGTT 
TCTCCAGAGC ATGTGAAGCA CTGTTTTAGA CTGACGCAAG AGTTTCGCCA CCTTTCCAAC 
ACTCACACAA ACCACGAGGA CAAGCTACAG GTGAAGAACA TCATCTACCA TGCTGTGAAG 
GATGCTGTGG CAACACTAAA AGCCCATGAG CCCAAATTAG GCCGTTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173199.1
CDS	267..5597
Translation	MGDSLGLIGKRLLLLLNDGSSAPAATGGEVERAAWLRGTVRAVSVIGLASPGVEVFVEFEDSPWRKRAWVQLYGDEVRVVLMESAIVWANCSDPSLSTALGSSATQWPALMFKQLIDRVGLGSVVPVEFFGARNLAFLPNGNSLHTFETEKDFTHSLLQEQPALQHAISSWHTDSELQEILRKGSYTIQGRRVKVYQPEFEQPWAFGLVSQHDPVSHIMEITMDQSCLKGEETQVVDPRVIHVMLAEGKLNESQDRRKKEGDGGKGEGSRRRRTASEGDEDITLKRFKGAGEGASDNQNGNSSNRDAEAMEHSHIRSTGFVKENGSFIPNQERISSVSAVLPASTPTPPPLKPAPSPFSNTFPSLGQMPNLVPGAPAPKSSPTLPSSEREEGGVLSGYPKTAALVSPGPVTISSPSQENTSSVALTAPVDANQKPSMWGSTSEGNQTPKAPVLAPAGFGKQSSGGVFGNVSTQTNGSSQGDKPFGFSFRGAKDSQRQDSDTSQNLFFQFISQNQSNTQGQSKAFTSLSECLNKEPPSLFKSAAPSEGFKKTVVASASTGLFGSAPASGLAPLKEQPKVPDIKPAGNGILMNKPFGAVGEAQSKLPATFSSAIIQAASSTEVLKPSGLGTSGLGNASGGIRNVNSSTPVSSFGLLAGNKVSDGHENLFLQPSKETNSFLAYGRGVPQTPFGALTSPKSSSAQSASAVSPSGSTSLLGQDPPGSDAKPNLFTMAEPPKGILAPQFAAPALMTTPSFTSVAQDGQQISKPNREGSDDSSGTLLSTEAEGVSVPFEQSKFSLEERLQSIKKDSESSSNSDLSDLSEGEDNAGQSQKPDLPAGNEDKNKAQAAAKSRPRSKPFKVGQSVLKDQNKVRRLKQSGEAFLQDGSCINVAPHLHKCRECRLERYRKSREQDSDDDDPNVACRFFHFRRLAFTKKGVLRVEGFLSPQQSDSMAMGLWLPSITIQEGLDLDTSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKNRPPEVEDGPEEEVFSWLKCAKGQPHEPQNLMPTQIIPGTALYSIGDMVHAARGKWGIKANCPCTSRHKTLMRPSAPNGLSQSGAAHSSGNGTTSTSSTTRPNEESAAGVVVKTEPVEEPTSADTTSSTSGTNSSTSSPAPAPTSAPTLLPPSLPPPSLPPPSLPPAPALVPTPPAKDSKSSSSALHWLADLATQKEPKESLRSMMGRDSRSPFGLDSFSTLSKPSGSSPKLFNSLLLGAGPSQPKAEGTSLRDLLNSGSGKLPQGPTEGGVPFPPVFSTASQADKMKGGLPNFLDHIIASVVETKKSEVRRASGSAEVIESATAPRREGVMGLSVLDPHTSHSWLCDGRLLCLQDPSNPNNWKIFRECWKQGQPVLVSGIHRKLKEHLWRPEAFSEEFGDQDVDLVNCRNCAIISDVKVREFWDGFQVISKRLQGSDGQPMVLKLKDWPPGEDFRDMMPTRFNDLMDNLPLPEYTKRDGRLNLASRLPNFFVRPDLGPKMYNAYGLISTEDRKVGTTNLHLDVSDAVNVMVYVGIPEGENDQESEADLAGFKEVMQTIEEGDVDDMTKRRVYEIKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRRRLYEEYGVQGWSIVQFLGDAVFIPAGAPHQVHNLYSCIKAAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVATLKAHEPKLGRS

Target ORF information:

RefSeq Version	XM_005173142.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 3B (kdm3b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173142.4

ORF Insert Sequence:

ATGGGGGACT CGCTCGGTCT GATCGGGAAG CGGCTGCTGT TGCTGCTCAA TGACGGGAGC 
TCGGCGCCTG CAGCGACGGG CGGAGAGGTG GAGCGGGCGG CCTGGCTCCG AGGGACCGTA 
CGGGCGGTCA GTGTGATCGG GCTGGCGAGC CCGGGGGTGG AGGTGTTTGT AGAATTTGAG 
GACAGCCCTT GGCGTAAGCG TGCATGGGTG CAGTTGTATG GGGATGAGGT GCGTGTTGTT 
CTCATGGAGA GTGCCATCGT TTGGGCAAAC TGCAGCGATC CTTCTCTCTC CACTGCTCTA 
GGATCATCTG CTACACAGTG GCCTGCCTTG ATGTTCAAAC AGCTGATTGA CCGTGTGGGT 
TTGGGGTCCG TGGTCCCTGT TGAGTTCTTT GGTGCAAGAA ATTTGGCGTT TCTCCCTAAT 
GGGAATTCGC TGCACACTTT TGAGACTGAA AAAGATTTTA CACACTCTCT GCTTCAAGAG 
CAGCCGGCAC TTCAACATGC CATTTCAAGC TGGCATACAG ACTCTGAGTT GCAGGAGATT 
TTGCGGAAAG GATCTTACAC TATTCAGGGT CGGAGAGTAA AGGTGTACCA GCCAGAGTTT 
GAGCAGCCCT GGGCCTTTGG ACTAGTATCT CAGCATGATC CTGTTTCACA CATTATGGAG 
ATTACCATGG ATCAGAGTTG TTTAAAAGGT GAGGAGACAC AAGTGGTTGA TCCTCGGGTT 
ATTCACGTAA TGCTTGCTGA AGGCAAGTTA AATGAGAGCC AAGATCGACG CAAAAAGGAG 
GGTGATGGTG GGAAAGGTGA GGGCAGTCGA AGACGTCGGA CAGCATCTGA AGGAGATGAA 
GACATTACAC TCAAACGTTT CAAAGGAGCC GGGGAAGGTG CCTCTGACAA CCAGAATGGG 
AATAGCTCCA ACAGGGACGC AGAGGCTATG GAACACTCCC ATATAAGGAG CACAGGTTTT 
GTGAAGGAGA ATGGCAGTTT TATCCCAAAC CAAGAAAGGA TTTCTTCAGT ATCTGCAGTG 
CTTCCTGCTT CTACTCCAAC GCCACCTCCT TTGAAGCCTG CCCCATCACC TTTTTCTAAC 
ACTTTTCCTT CTTTGGGTCA AATGCCCAAC TTGGTGCCCG GGGCCCCTGC CCCGAAATCA 
TCTCCCACAC TCCCATCGTC AGAGAGGGAG GAGGGAGGTG TTCTTTCAGG GTATCCTAAA 
ACAGCTGCCC TGGTGTCTCC AGGTCCTGTG ACCATTTCAT CACCTTCCCA AGAAAACACT 
TCAAGTGTGG CTCTCACTGC TCCTGTTGAT GCGAACCAAA AGCCCAGTAT GTGGGGATCA 
ACCTCAGAAG GAAATCAGAC CCCTAAAGCC CCTGTTCTAG CTCCTGCTGG ATTTGGTAAA 
CAGTCCAGTG GAGGGGTGTT TGGGAATGTC TCAACACAGA CTAATGGATC TTCCCAGGGG 
GACAAACCCT TTGGGTTTTC ATTTAGAGGT GCAAAGGACT CGCAAAGGCA GGACTCTGAT 
ACGTCACAGA ACCTGTTCTT CCAATTCATC TCTCAGAACC AGAGCAACAC ACAAGGCCAA 
TCAAAAGCCT TCACATCTTT GTCCGAGTGC CTGAACAAGG AGCCGCCCAG CCTGTTCAAG 
TCTGCTGCTC CCTCTGAGGG TTTCAAAAAA ACTGTTGTGG CTTCTGCATC CACTGGACTG 
TTTGGTTCAG CACCTGCCAG TGGCCTGGCA CCACTGAAAG AGCAGCCAAA AGTGCCTGAT 
ATCAAGCCTG CAGGCAATGG GATCCTTATG AACAAGCCTT TTGGTGCTGT AGGGGAGGCA 
CAGAGCAAAC TCCCAGCCAC TTTTTCTTCT GCCATAATTC AAGCTGCAAG CTCTACAGAG 
GTGCTGAAAC CCTCGGGATT AGGAACCAGT GGACTTGGAA ATGCCAGTGG TGGAATTAGA 
AATGTAAATA GCTCAACCCC AGTGAGTAGC TTTGGTCTGC TTGCTGGCAA CAAGGTTTCC 
GATGGACACG AGAACCTGTT CTTGCAGCCC TCGAAGGAGA CAAATTCGTT TCTTGCCTAT 
GGCAGAGGTG TTCCGCAAAC CCCTTTTGGT GCATTGACAT CTCCAAAGTC TTCATCTGCC 
CAGTCCGCAT CTGCTGTTTC ACCTTCAGGC AGCACCAGTC TGCTCGGTCA AGATCCTCCT 
GGTAGTGATG CAAAACCAAA TCTCTTCACT ATGGCAGAAC CTCCCAAGGG AATCTTAGCC 
CCTCAGTTTG CAGCTCCTGC TCTCATGACC ACTCCGTCAT TCACATCAGT TGCACAGGAT 
GGACAGCAAA TATCCAAACC AAACAGAGAA GGATCAGATG ACAGCTCTGG AACCCTTCTC 
AGCACTGAAG CAGAAGGTGT CTCTGTGCCC TTTGAGCAAA GCAAGTTTTC ATTGGAGGAA 
CGTCTTCAGT CTATCAAAAA GGATTCAGAG TCCAGTAGTA ACAGTGACTT GTCTGATTTG 
AGTGAGGGAG AGGACAACGC TGGCCAGAGC CAGAAACCCG ATCTTCCGGC AGGCAATGAG 
GATAAGAACA AGGCCCAGGC TGCAGCCAAG AGCCGGCCAC GGAGCAAACC ATTTAAAGTT 
GGACAGTCTG TGTTGAAGGA CCAGAATAAG GTACGGCGCT TGAAGCAGTC TGGAGAGGCC 
TTTCTACAAG ATGGCTCCTG CATTAACGTG GCTCCCCACT TGCACAAATG TCGTGAGTGC 
CGTCTGGAGC GCTACAGGAA ATCGCGTGAG CAGGACTCTG ATGATGACGA CCCAAATGTA 
GCCTGTCGAT TCTTTCACTT TCGCAGGTTG GCTTTCACTA AAAAGGGAGT TCTTCGTGTG 
GAGGGTTTTC TGAGCCCACA GCAGAGTGAC AGCATGGCAA TGGGCCTGTG GCTTCCTTCC 
ATCACAATAC AAGAAGGACT TGACCTGGAC ACGTCCAAAT ATATTTTAGC AAATGTGGGG 
GACCAGTTTT GCCAATTGGT CATGTCAGAG AAAGAAGCCA TGATGATGGT AGAACCACAT 
CAAAAAGTGG CTTGGAAGCG AGCTGTCCGA GGAGTGAGAG AAATGTGTGA TGTTTGTGAA 
ACAACCCTGT TCAACATCCA CTGGGTTTGT CGTAAATGTG GCTTCGGCGT TTGTCTTGAC 
TGCTATCGAC TACGCAAGAA CAGGCCACCT GAAGTGGAAG ATGGTCCTGA AGAGGAGGTG 
TTCTCATGGC TAAAGTGTGC TAAAGGCCAG CCACACGAAC CACAGAACCT CATGCCTACT 
CAAATTATAC CCGGCACGGC ATTGTACAGC ATAGGTGACA TGGTACATGC AGCCAGAGGT 
AAATGGGGGA TTAAAGCAAA CTGTCCCTGC ACTAGTCGGC ACAAAACGTT GATGCGGCCC 
AGTGCTCCAA ATGGTCTTTC ACAGTCGGGT GCAGCTCACA GTTCGGGGAA TGGAACCACG 
TCTACTTCAT CCACAACCCG TCCAAATGAA GAATCTGCAG CTGGTGTTGT AGTCAAAACT 
GAACCCGTTG AAGAGCCTAC AAGTGCTGAT ACCACATCAA GTACCAGTGG GACAAATAGC 
AGCACATCTA GCCCTGCTCC TGCTCCAACT TCTGCTCCTA CTCTTCTTCC TCCTTCTCTT 
CCTCCTCCTT CACTTCCTCC TCCTTCACTT CCTCCTGCTC CTGCACTTGT ACCTACACCT 
CCTGCAAAGG ATAGCAAGAG CAGTTCTTCA GCACTTCACT GGCTGGCAGA TCTGGCCACA 
CAAAAGGAGC CCAAAGAGTC TCTGCGCTCC ATGATGGGTC GTGACTCTCG TTCTCCATTT 
GGCCTGGACT CGTTCAGCAC ACTTTCCAAA CCATCAGGGT CCAGTCCTAA GCTGTTCAAC 
AGTCTGCTGC TGGGTGCAGG CCCTTCACAA CCCAAAGCAG AGGGAACCAG CCTCCGAGAT 
CTGCTGAACT CTGGCTCTGG CAAACTCCCG CAAGGGCCCA CAGAGGGAGG TGTCCCATTT 
CCTCCAGTGT TCTCCACTGC TAGTCAGGCT GATAAAATGA AGGGAGGTCT TCCTAACTTC 
CTGGATCATA TCATTGCATC AGTAGTGGAG ACTAAGAAGT CAGAGGTTCG TCGTGCATCA 
GGTTCAGCAG AAGTCATCGA GTCTGCCACT GCTCCTCGCA GAGAGGGGGT GATGGGGCTG 
AGTGTCCTGG ATCCACACAC TTCACACTCT TGGCTTTGTG ACGGCCGTTT GCTCTGCCTG 
CAGGACCCCA GTAACCCCAA CAACTGGAAG ATCTTCAGAG AGTGCTGGAA ACAAGGACAG 
CCTGTGCTGG TCTCAGGCAT ACACAGGAAG CTGAAGGAAC ACTTATGGAG GCCTGAGGCC 
TTCAGCGAGG AGTTTGGAGA CCAGGACGTG GACCTGGTCA ACTGTAGGAA CTGCGCCATC 
ATCTCTGATG TTAAAGTCAG GGAATTTTGG GATGGCTTCC AGGTCATTTC CAAGCGATTG 
CAAGGTAGTG ATGGCCAGCC TATGGTACTA AAACTTAAAG ACTGGCCTCC TGGAGAAGAT 
TTTCGAGATA TGATGCCCAC TCGGTTTAAT GATCTCATGG ACAATCTTCC TTTGCCCGAG 
TACACAAAGA GAGATGGCCG TCTCAACCTG GCCTCTCGTC TTCCAAACTT CTTTGTTCGG 
CCAGATCTGG GCCCTAAAAT GTACAATGCG TATGGTCTGA TCTCCACAGA AGACAGGAAG 
GTCGGCACCA CTAACTTGCA TTTGGATGTG TCTGATGCCG TCAACGTTAT GGTGTATGTG 
GGCATCCCAG AAGGAGAGAA TGACCAAGAA AGTGAAGCAG ACCTCGCTGG ATTCAAAGAG 
GTGATGCAGA CTATTGAAGA GGGTGATGTA GATGACATGA CGAAAAGAAG AGTCTACGAG 
ATCAAGGAGA AACCTGGGGC TCTCTGGCAC ATCTATGCTG CCAAAGACGC TGAGAAGATC 
CGAGAGCTCT TACGAAAGGT TGGAGAGGAA CAAGGTCAAG AGAACCCACC AGACCATGAT 
CCTATCCATG ACCAGAGCTG GTACCTGGAC CAGACGTTGC GTCGCAGGCT GTATGAGGAG 
TATGGAGTCC AGGGATGGTC CATTGTGCAG TTTTTGGGAG ATGCAGTGTT CATCCCAGCT 
GGAGCACCAC ATCAGGTGCA CAACCTGTAC AGCTGTATCA AAGCTGCAGA AGATTTTGTT 
TCTCCAGAGC ATGTGAAGCA CTGTTTTAGA CTGACGCAAG AGTTTCGCCA CCTTTCCAAC 
ACTCACACAA ACCACGAGGA CAAGCTACAG GTGAAGAACA TCATCTACCA TGCTGTGAAG 
GATGCTGTGG CAACACTAAA AGCCCATGAG CCCAAATTAG GCCGTTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	kdm3b
Entrez Gene ID	326643
Full Name	lysine (K)-specific demethylase 3B
Synonyms	im:7163548,wu:fa12c09,wu:fe01c10
General protein information	Preferred Names lysine (K)-specific demethylase 3B Names lysine-specific demethylase 3B wu:fe01c10 fa12c09 fe01c10
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Pan troglodytes (chimpanzee)	KDM3B	XP_517956.3
Mus musculus (house mouse)	Kdm3b	NP_001074725.1
Danio rerio (zebrafish)	wu:fe01c10	XP_005173195.1
	KDM3B	XP_002804573.1
Gallus gallus (chicken)	KDM3B	XP_004945121.1
Xenopus tropicalis (tropical clawed frog)	kdm3b	XP_002936894.1
Bos taurus (cattle)	KDM3B	XP_003586349.1
Homo sapiens (human)	KDM3B	NP_057688.2
Canis lupus familiaris (dog)	KDM3B	XP_531921.3
Rattus norvegicus (Norway rat)	Kdm3b	XP_006254677.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

kdm3b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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