clr4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	clr4
Entrez Gene ID	2540825
Full Name	histone H3 methyltransferase Clr4
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021094.2	NP_595186.1	histone H3 methyltransferase Clr4

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	clr4	NP_595186.1
Danio rerio (zebrafish)	suv39h1a	NP_001003592.1
Magnaporthe oryzae (rice blast fungus)	MGG_06852	XP_003709573.1
Neurospora crassa	NCU04402	XP_957479.2
Drosophila melanogaster (fruit fly)	Su(var)3-9	NP_524357.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Survival in quiescence requires the euchromatic deployment of Clr4 by Ago1-associated small RNAs.
Title: Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

These results demonstrate how a direct "read-write" mechanism involving Clr4 propagates histone modifications and allows histones to act as carriers of epigenetic information.
Title: Epigenetics. Epigenetic inheritance uncoupled from sequence-specific recruitment.

Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly.
Title: Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly in fission yeast.

Division of Labor between the Chromodomains of HP1/Swi6 and Suv39/Clr4 Is Required for Productive Heterochromatin Assembly.
Title: Division of labor between the chromodomains of HP1 and Suv39 methylase enables coordination of heterochromatin spread.

Data show that although Ers1 failed to bind Clr4, a direct interaction with Swi6 was detected, and centromeric localization of Swi6 was enhanced by Clr4 overexpression.
Title: Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.

The following clr4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clr4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100999	NM_001021094.2 Latest version!	Schizosaccharomyces pombe histone H3 methyltransferase Clr4 (clr4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clr4 ( NM_001021094.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595186.1 Schizosaccharomyces pombe histone H3 methyltransferase Clr4 (clr4), mRNA.

Price & Availability↑

CloneID	OSc100999
Clone ID Related Accession (Same CDS sequence)	NM_001021094.2
Accession Version	NM_001021094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	histone H3 methyltransferase Clr4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021094.1.

ATGTCGCCTA AACAAGAGGA GTATGAAGTT GAAAGGATTG TGGACGAAAA ATTGGATCGT 
AATGGAGCTG TCAAACTATA TCGCATTCGA TGGTTGAACT ATTCATCTAG AAGTGATACT 
TGGGAGCCCC CTGAGAACCT TTCTGGATGT TCTGCCGTTT TAGCAGAATG GAAAAGGCGG 
AAGAGGAGAC TAAAGGGAAG TAACTCCGAC TCGGATTCAC CGCACCATGC GAGCAATCCA 
CATCCAAACA GTAGACAAAA GCACCAACAT CAAACTTCAA AGTCAGTGCC TCGTTCTCAA 
AGATTTTCGC GGGAATTGAA TGTAAAAAAG GAAAATAAAA AGGTTTTTTC TTCTCAAACT 
ACCAAGAGAC AATCTCGAAA ACAATCCACC GCTCTGACGA CGAATGATAC TAGTATCATT 
TTAGACGATA GTCTTCATAC AAATTCAAAG AAGCTGGGGA AGACGAGAAA TGAAGTGAAG 
GAGGAATCTC AAAAAAGAGA ACTAGTTTCC AATTCTATTA AAGAAGCTAC ATCCCCCAAA 
ACATCTTCAA TTCTCACAAA ACCGCGAAAC CCTTCCAAAC TTGACTCTTA TACCCATCTA 
AGTTTTTACG AAAAGAGAGA GCTATTTCGT AAGAAACTGC GGGAAATTGA AGGACCCGAA 
GTAACGCTTG TCAATGAAGT TGACGATGAA CCCTGTCCAT CTTTAGATTT TCAATTCATT 
AGCCAGTACC GCTTAACACA AGGTGTTATC CCGCCTGATC CAAATTTTCA ATCTGGATGC 
AATTGCTCGT CCCTTGGCGG TTGTGACCTT AACAATCCTT CTCGTTGTGA GTGTTTAGAT 
GATTTGGATG AGCCAACTCA TTTTGCCTAT GATGCACAGG GGCGGGTCCG TGCAGATACG 
GGTGCCGTTA TATATGAATG CAACTCTTTT TGTTCTTGTT CAATGGAGTG TCCTAATCGC 
GTTGTTCAAC GTGGTAGAAC TCTTCCGCTT GAAATATTTA AAACAAAAGA AAAAGGATGG 
GGTGTACGTT CGCTTCGATT TGCCCCTGCT GGCACGTTTA TCACTTGCTA TTTAGGAGAA 
GTCATAACTT CAGCAGAGGC CGCCAAGCGT GACAAAAATT ATGACGACGA CGGTATTACC 
TATTTATTTG ATTTAGACAT GTTTGATGAT GCTAGCGAGT ATACAGTGGA TGCTCAGAAC 
TATGGAGATG TCTCTCGTTT TTTTAACCAC TCCTGTTCAC CTAATATAGC TATTTATTCT 
GCTGTTCGGA ACCATGGATT TCGAACCATT TATGATCTCG CTTTTTTTGC CATCAAAGAC 
ATTCAGCCTC TAGAGGAGCT TACATTTGAT TACGCTGGTG CAAAAGATTT CTCACCTGTT 
CAATCTCAAA AATCTCAGCA AAATAGAATT TCAAAACTTC GCCGGCAGTG CAAATGTGGT 
TCCGCTAATT GTCGTGGCTG GCTTTTCGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595186.1
CDS	441..1913
Translation	MSPKQEEYEVERIVDEKLDRNGAVKLYRIRWLNYSSRSDTWEPPENLSGCSAVLAEWKRRKRRLKGSNSDSDSPHHASNPHPNSRQKHQHQTSKSVPRSQRFSRELNVKKENKKVFSSQTTKRQSRKQSTALTTNDTSIILDDSLHTNSKKLGKTRNEVKEESQKRELVSNSIKEATSPKTSSILTKPRNPSKLDSYTHLSFYEKRELFRKKLREIEGPEVTLVNEVDDEPCPSLDFQFISQYRLTQGVIPPDPNFQSGCNCSSLGGCDLNNPSRCECLDDLDEPTHFAYDAQGRVRADTGAVIYECNSFCSCSMECPNRVVQRGRTLPLEIFKTKEKGWGVRSLRFAPAGTFITCYLGEVITSAEAAKRDKNYDDDGITYLFDLDMFDDASEYTVDAQNYGDVSRFFNHSCSPNIAIYSAVRNHGFRTIYDLAFFAIKDIQPLEELTFDYAGAKDFSPVQSQKSQQNRISKLRRQCKCGSANCRGWLFG

Target ORF information:

RefSeq Version	NM_001021094.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe histone H3 methyltransferase Clr4 (clr4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021094.2

ORF Insert Sequence:

ATGTCGCCTA AACAAGAGGA GTATGAAGTT GAAAGGATTG TGGACGAAAA ATTGGATCGT 
AATGGAGCTG TCAAACTATA TCGCATTCGA TGGTTGAACT ATTCATCTAG AAGTGATACT 
TGGGAGCCCC CTGAGAACCT TTCTGGATGT TCTGCCGTTT TAGCAGAATG GAAAAGGCGG 
AAGAGGAGAC TAAAGGGAAG TAACTCCGAC TCGGATTCAC CGCACCATGC GAGCAATCCA 
CATCCAAACA GTAGACAAAA GCACCAACAT CAAACTTCAA AGTCAGTGCC TCGTTCTCAA 
AGATTTTCGC GGGAATTGAA TGTAAAAAAG GAAAATAAAA AGGTTTTTTC TTCTCAAACT 
ACCAAGAGAC AATCTCGAAA ACAATCCACC GCTCTGACGA CGAATGATAC TAGTATCATT 
TTAGACGATA GTCTTCATAC AAATTCAAAG AAGCTGGGGA AGACGAGAAA TGAAGTGAAG 
GAGGAATCTC AAAAAAGAGA ACTAGTTTCC AATTCTATTA AAGAAGCTAC ATCCCCCAAA 
ACATCTTCAA TTCTCACAAA ACCGCGAAAC CCTTCCAAAC TTGACTCTTA TACCCATCTA 
AGTTTTTACG AAAAGAGAGA GCTATTTCGT AAGAAACTGC GGGAAATTGA AGGACCCGAA 
GTAACGCTTG TCAATGAAGT TGACGATGAA CCCTGTCCAT CTTTAGATTT TCAATTCATT 
AGCCAGTACC GCTTAACACA AGGTGTTATC CCGCCTGATC CAAATTTTCA ATCTGGATGC 
AATTGCTCGT CCCTTGGCGG TTGTGACCTT AACAATCCTT CTCGTTGTGA GTGTTTAGAT 
GATTTGGATG AGCCAACTCA TTTTGCCTAT GATGCACAGG GGCGGGTCCG TGCAGATACG 
GGTGCCGTTA TATATGAATG CAACTCTTTT TGTTCTTGTT CAATGGAGTG TCCTAATCGC 
GTTGTTCAAC GTGGTAGAAC TCTTCCGCTT GAAATATTTA AAACAAAAGA AAAAGGATGG 
GGTGTACGTT CGCTTCGATT TGCCCCTGCT GGCACGTTTA TCACTTGCTA TTTAGGAGAA 
GTCATAACTT CAGCAGAGGC CGCCAAGCGT GACAAAAATT ATGACGACGA CGGTATTACC 
TATTTATTTG ATTTAGACAT GTTTGATGAT GCTAGCGAGT ATACAGTGGA TGCTCAGAAC 
TATGGAGATG TCTCTCGTTT TTTTAACCAC TCCTGTTCAC CTAATATAGC TATTTATTCT 
GCTGTTCGGA ACCATGGATT TCGAACCATT TATGATCTCG CTTTTTTTGC CATCAAAGAC 
ATTCAGCCTC TAGAGGAGCT TACATTTGAT TACGCTGGTG CAAAAGATTT CTCACCTGTT 
CAATCTCAAA AATCTCAGCA AAATAGAATT TCAAAACTTC GCCGGCAGTG CAAATGTGGT 
TCCGCTAATT GTCGTGGCTG GCTTTTCGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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clr4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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