SPBC30D10.07c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPBC30D10.07c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC30D10.07c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100281 NM_001022199.2
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Schizosaccharomyces pombe putative biotin-protein ligase (SPBC30D10.07c), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100281
    Clone ID Related Accession (Same CDS sequence) NM_001022199.2
    Accession Version NM_001022199.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative biotin-protein ligase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022199.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAATGTTT TAATATACAA TGGCAATGGG GCCTCCAAAA TATGCCTACT GAGAACATTT 
    CAATCCCTTT TACCATTTGT CGTTCCGCTA TATGCAATGA GATTTGTCGA CGCCTCTACT
    TTAGAAAAAG AACCTTGGCC TGCTAGCACA GCTTTGTTAG TTATGCCCGG CGGAAGAGAT
    ATGGGTTACT GTTCTTCTTT TAATGAAACA ATTTACCGGA AAATTACTGA TTTTGTCAAG
    CGAGGGGGAG CTTATCTGGG TTTATGTGCA GGAGGATATT TTGGTTCTGC GGTGGTTGAT
    TTTCGAATGC CAGACTCTGA TTTGAACGTT GTAGGGAAAA GGAAACTTCA ATTTTTTCCA
    GGAACTTGTG CTGGTCCTAC CTTTCCTGGA TTTACCTATG ATAGCGAGGA TGGGGCGCGA
    CGGGCATCTA TAATTGTTGA TGGCATGCAA TCATCCCCCG TTCATACCCA CATATATTTT
    AATGGAGGTG GGTCTTTTTT GGAGACAGAA AACTATTCTA ATGTTAAGGT GGTTGCTCGC
    TATCAAGAGA CAGATTTTGA GAAATCAGCA GCTATAATTT ATGTTAAAGT AGGAAAGGGA
    AACGTTGTAC TTACAGGTAT TCATCCTGAG TTTTCAGCAG AGGGAAGCCC CATTTTAGAT
    AAAAGAGATG AGAAAACACG ATTAGAATTG TTATCCTATA TTCTCAAACT CCTTGGGTTG
    AAGGTTCCTA AGGATACATC TAAATGTGGT CAGCCTACAT TAACTGATCA GTACTTGTTC
    CCGAATAATG TGGAGACTAA AAGATTTATT GAAAAAGCTC TTACAAATAA GGTAAAAAAT
    CAAGATGAGG ACACTCTGTA TACCTTCCAG TTTTCCGACA TAAGTTCTGA AATCCCGGAG
    CATCAACTTG CTAATTTGGA TATTTCCGCC GATTTGTCAG ATTCTGATAA TGAAATTGTA
    AAAATATGGT ATGGTGACGA AGAAAAAATA TGCAAAAAAG CGAAGCCTAG TTTTGATTTA
    GAGCTTTATG CAAAGTTAAT TAACGGATGT CGTTTCGGTT TACCAATTAT AGTTGCACCA
    GTAATCCGGT CAACTCAAAC TCTACTAGAC AAAAACTATA GATTTTTGGA TTCAACGAAT
    ACTGGTTTTA CCGTGTTGGG TAATTACCAG ACTGCTGGTC GAGGCCGAGG TCAAAACATG
    TGGGTCTCTC CCTATGGAAC GCTCGCGTTT TCGTTCATCA TCAACGTGGA TGCTAAAAAT
    TTCTCTACTA CACCTATAGC GCTTTTTCAG TATTTGATGG CCTTAGCAGT TGTCCGTGGT
    ATTCGTGAGT ATGCTCCAGG CTATGAAAAT ATTCCTGCAT TTATCAAGTG GCCTAACGAT
    GTTTATGTGC GTGTTGATAA AGGTGGAATA AATTTTCAAG GTAAGCAGTA TATGAAGCTT
    TCTGGAATCA TAGTTACTTC TAATTACCGA AAAAATGTCT TACATTTGGT TGTTGGCTGT
    GGAATAAATG TCAGTAACTT GGGTCCTACT GTTTCTTTGA ATACTCTGGT AGATGAATGG
    AACAAAAACT CTGATAATCC GCGGCTAGAG AAGTTTTCCT TTGAAAAGCT GCTTGCCAGT
    GTACTCAATC AGTTTGATCG TTATCATAGG TTACTTTTGG AAGAGGGTTT TTCTCTTATT
    TTACCAGAAT ATTACCAATA TTGGTTACAT AGCAACCAAA CGGTCAATTT AGCAAGCGGC
    GGTAAAGCAA TAATTCAAGG GATTACCTCT GATTTCGGAT TTCTGTTAGC TCAACTGTTA
    AACGAAAACA ATGAGCCTAC AACGAAAGTA GTTCACTTGC AACCTGACGG CAACAGCTTT
    GATCTGATGA GAAATTTAAT AACAAGAAAA ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596278.1
    CDS199..2094
    Translation

    Target ORF information:

    RefSeq Version NM_001022199.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative biotin-protein ligase (SPBC30D10.07c), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022199.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAATGTTT TAATATACAA TGGCAATGGG GCCTCCAAAA TATGCCTACT GAGAACATTT 
    CAATCCCTTT TACCATTTGT CGTTCCGCTA TATGCAATGA GATTTGTCGA CGCCTCTACT
    TTAGAAAAAG AACCTTGGCC TGCTAGCACA GCTTTGTTAG TTATGCCCGG CGGAAGAGAT
    ATGGGTTACT GTTCTTCTTT TAATGAAACA ATTTACCGGA AAATTACTGA TTTTGTCAAG
    CGAGGGGGAG CTTATCTGGG TTTATGTGCA GGAGGATATT TTGGTTCTGC GGTGGTTGAT
    TTTCGAATGC CAGACTCTGA TTTGAACGTT GTAGGGAAAA GGAAACTTCA ATTTTTTCCA
    GGAACTTGTG CTGGTCCTAC CTTTCCTGGA TTTACCTATG ATAGCGAGGA TGGGGCGCGA
    CGGGCATCTA TAATTGTTGA TGGCATGCAA TCATCCCCCG TTCATACCCA CATATATTTT
    AATGGAGGTG GGTCTTTTTT GGAGACAGAA AACTATTCTA ATGTTAAGGT GGTTGCTCGC
    TATCAAGAGA CAGATTTTGA GAAATCAGCA GCTATAATTT ATGTTAAAGT AGGAAAGGGA
    AACGTTGTAC TTACAGGTAT TCATCCTGAG TTTTCAGCAG AGGGAAGCCC CATTTTAGAT
    AAAAGAGATG AGAAAACACG ATTAGAATTG TTATCCTATA TTCTCAAACT CCTTGGGTTG
    AAGGTTCCTA AGGATACATC TAAATGTGGT CAGCCTACAT TAACTGATCA GTACTTGTTC
    CCGAATAATG TGGAGACTAA AAGATTTATT GAAAAAGCTC TTACAAATAA GGTAAAAAAT
    CAAGATGAGG ACACTCTGTA TACCTTCCAG TTTTCCGACA TAAGTTCTGA AATCCCGGAG
    CATCAACTTG CTAATTTGGA TATTTCCGCC GATTTGTCAG ATTCTGATAA TGAAATTGTA
    AAAATATGGT ATGGTGACGA AGAAAAAATA TGCAAAAAAG CGAAGCCTAG TTTTGATTTA
    GAGCTTTATG CAAAGTTAAT TAACGGATGT CGTTTCGGTT TACCAATTAT AGTTGCACCA
    GTAATCCGGT CAACTCAAAC TCTACTAGAC AAAAACTATA GATTTTTGGA TTCAACGAAT
    ACTGGTTTTA CCGTGTTGGG TAATTACCAG ACTGCTGGTC GAGGCCGAGG TCAAAACATG
    TGGGTCTCTC CCTATGGAAC GCTCGCGTTT TCGTTCATCA TCAACGTGGA TGCTAAAAAT
    TTCTCTACTA CACCTATAGC GCTTTTTCAG TATTTGATGG CCTTAGCAGT TGTCCGTGGT
    ATTCGTGAGT ATGCTCCAGG CTATGAAAAT ATTCCTGCAT TTATCAAGTG GCCTAACGAT
    GTTTATGTGC GTGTTGATAA AGGTGGAATA AATTTTCAAG GTAAGCAGTA TATGAAGCTT
    TCTGGAATCA TAGTTACTTC TAATTACCGA AAAAATGTCT TACATTTGGT TGTTGGCTGT
    GGAATAAATG TCAGTAACTT GGGTCCTACT GTTTCTTTGA ATACTCTGGT AGATGAATGG
    AACAAAAACT CTGATAATCC GCGGCTAGAG AAGTTTTCCT TTGAAAAGCT GCTTGCCAGT
    GTACTCAATC AGTTTGATCG TTATCATAGG TTACTTTTGG AAGAGGGTTT TTCTCTTATT
    TTACCAGAAT ATTACCAATA TTGGTTACAT AGCAACCAAA CGGTCAATTT AGCAAGCGGC
    GGTAAAGCAA TAATTCAAGG GATTACCTCT GATTTCGGAT TTCTGTTAGC TCAACTGTTA
    AACGAAAACA ATGAGCCTAC AACGAAAGTA GTTCACTTGC AACCTGACGG CAACAGCTTT
    GATCTGATGA GAAATTTAAT AACAAGAAAA ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L