NCU05516 cDNA ORF clone, Neurospora crassa OR74A

The following NCU05516 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU05516 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf06183 XM_955385.2
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Neurospora crassa OR74A biotin apo-protein ligase (NCU05516), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf06183
    Clone ID Related Accession (Same CDS sequence) XM_955385.2
    Accession Version XM_955385.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product biotin apo-protein ligase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506). On Feb 23, 2015 this sequence version replaced XM_955385.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTACCC GCCTGAAAAC TGACCAGCGG GTACTCAATG TCCTGATCTA CGCAGGAGCG 
    GGAGTAACCC CCGAATGTCT CCGCCAATGC ACCGAGTCCC TGAAGCGTCT CGTGGGATGC
    AAATATGCTG TTTCGCACAT AACAGCATCG ACACTCAAGA CCGAACCCTG GCAGTCAACT
    TGTGCTCTGT TGGTTTTTCC TGGTGGCGCC GACCTCCCCT TCTGTCGCGA CCTCAACGGC
    CCCGGCAACC GCGTCATTGC CGACTACGTC CGCAACGGAG GAGCTTACCT GGGCTTCTGT
    GCCGGAGGCT ACTATGGCAG CGGCCAGTGT TGGTTCCAGC TCGACGACCC AAGCCAGGAT
    GTGAAGGGCC CGAGAGAGCT GGCTTTCTTC CCAGGCACTT GCGGTGGTCC TGCCTTCAAG
    GGATTCCAAT ACCACTGTGA AATGGGCGCC AAGGCTGCCA AACTTTCTGT GAGAAAGGAA
    GCCTTCACTG GTGGCCAGGC GCCGCCTGAT GTCTTCAGAT CGTACTACAA TGGAGGCGGA
    GTCTTCCTCA ATGCCGAGGC GATGGAATCC GACGGGGTCG AAATCCTTGC CGAATACGCA
    GACCATATCG ATTTCCCGTC TGGCAAGACA CAGGCTGCTG TGGTATTTTG CAAGGTTGGC
    CAGGGCAAAG CCATACTCAC AGGACCACAC CCAGAATTTT CTGGCACTTC TTTGAAATCG
    CAGCCTGACA TCCCGGGTTA TGACAAGCTT GTAGAGGAGG TGACGGCGGA CGAAAGTGCG
    CGACTGGAAT TTCTTGCGGC GTGTCTCAGC AAGATGGGGT TGTCACTCTC TCCCAAGGCT
    GCATGGGATG ACATTCCCCG ACTAACGACC ATGTTGAACA TCTACTGCCA GGGAGGGCAC
    CTTGAGTCCC TCCTGAACCG CCTCACGGGG CGAAGCTATG CGGTCATAAC CGATGGGAGC
    ATCTGGCTCG AGGATAAGTA CGGATCGGAT ATGTTCCATC TCGAGTGGAC TACTGACGGC
    TCATGGTCAA CCAACTACGA ACCACGAAAG TCTGACAAAG TTACTTGGCG ACTCAATGTT
    GGCTCTAAGG TAGCAGGTCA AGGGGACGAC GAGTCAGGCA CAGAGTTTGA CCACGACATT
    TACTACGCCA GCCTGGAAGA CTTTAGAATG CTTGAATCCA ACCGTGGGTT CAGTACATGG
    AAGCAAGCAT TGCAAGTGGG GACACAGGGC CCATGGTGTC CGACCCTAAG CTGGGGCAAC
    CACTTGATGT ATGGGGACAC TGTCACGAGT ACTAATACTT TGCTCTCAAG CAACCCCCAA
    CTTCTTTCCA AACTAGAGTC CGGCTTCACC TTTACCGCCA CTCGCCAGGT GGCAGGCCGT
    GGCCGGGGCA ACAACGTCTG GGTCTCCCCC GCCGGCTGCT TGATAATGTC AACGGTCATC
    AACCACTCAG CGAATGTCAT TGCTTCACGC CCGTTAGGCT TCTTCAATTA CCTGGCGGCG
    ATTGCTATCG TCGAGGCGAT CAAGGGATAC GACCACGAGG ATGACATTTA CCAAAAGTTG
    GACGTCAAGA TCAAGTGGCC GAACGACATC TACGTTCGAG ACCCGAGCAA GCCCAACGAG
    CCAGCCTACG TCAAAGTTGG CGGTATCCTC GCCAACTGTT CTTACAGCGC TCCGAATTAT
    CAGGTTGTCC TCGGCATCGG ACTCAACACG AACAACGCCC AACCCACGAC CAGTCTCGAT
    GCACTTCTTA AGTGGACTGC TAGCGTGCTA CAAGGCGACA AGCCACCTCC ACCGCCGTTC
    CACATAGAAC GCCTGGTGGC CAGAATTGTC ACCTGCCTCG AGATCCTCTA CGACCACTTC
    CTGCGGAAGG GGTTCTCGGA CGAGGTGGAA GCCTTGTACT ACAAGCATTG GCTGCATACC
    AACCAGATCG TCACCCTTGA AGATGAAGAC GGCGTGAAGG CCCGCGTACT CGGTATCACC
    AAGGATCAAG GCCTTCTGGT GGCCGAGGAG GTCATGGAGG ACGGTATCGA ACTGAACTCA
    TGGAAGGGAA CGGGCACAAT GTTCCAGTTG CAGAGCGACG AGAACGGGTT CGACTACTGG
    AAGGGACTGA TCAAGAGGAA GGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_960478.1
    CDS502..2628
    Translation

    Target ORF information:

    RefSeq Version XM_955385.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A biotin apo-protein ligase (NCU05516), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_955385.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTACCC GCCTGAAAAC TGACCAGCGG GTACTCAATG TCCTGATCTA CGCAGGAGCG 
    GGAGTAACCC CCGAATGTCT CCGCCAATGC ACCGAGTCCC TGAAGCGTCT CGTGGGATGC
    AAATATGCTG TTTCGCACAT AACAGCATCG ACACTCAAGA CCGAACCCTG GCAGTCAACT
    TGTGCTCTGT TGGTTTTTCC TGGTGGCGCC GACCTCCCCT TCTGTCGCGA CCTCAACGGC
    CCCGGCAACC GCGTCATTGC CGACTACGTC CGCAACGGAG GAGCTTACCT GGGCTTCTGT
    GCCGGAGGCT ACTATGGCAG CGGCCAGTGT TGGTTCCAGC TCGACGACCC AAGCCAGGAT
    GTGAAGGGCC CGAGAGAGCT GGCTTTCTTC CCAGGCACTT GCGGTGGTCC TGCCTTCAAG
    GGATTCCAAT ACCACTGTGA AATGGGCGCC AAGGCTGCCA AACTTTCTGT GAGAAAGGAA
    GCCTTCACTG GTGGCCAGGC GCCGCCTGAT GTCTTCAGAT CGTACTACAA TGGAGGCGGA
    GTCTTCCTCA ATGCCGAGGC GATGGAATCC GACGGGGTCG AAATCCTTGC CGAATACGCA
    GACCATATCG ATTTCCCGTC TGGCAAGACA CAGGCTGCTG TGGTATTTTG CAAGGTTGGC
    CAGGGCAAAG CCATACTCAC AGGACCACAC CCAGAATTTT CTGGCACTTC TTTGAAATCG
    CAGCCTGACA TCCCGGGTTA TGACAAGCTT GTAGAGGAGG TGACGGCGGA CGAAAGTGCG
    CGACTGGAAT TTCTTGCGGC GTGTCTCAGC AAGATGGGGT TGTCACTCTC TCCCAAGGCT
    GCATGGGATG ACATTCCCCG ACTAACGACC ATGTTGAACA TCTACTGCCA GGGAGGGCAC
    CTTGAGTCCC TCCTGAACCG CCTCACGGGG CGAAGCTATG CGGTCATAAC CGATGGGAGC
    ATCTGGCTCG AGGATAAGTA CGGATCGGAT ATGTTCCATC TCGAGTGGAC TACTGACGGC
    TCATGGTCAA CCAACTACGA ACCACGAAAG TCTGACAAAG TTACTTGGCG ACTCAATGTT
    GGCTCTAAGG TAGCAGGTCA AGGGGACGAC GAGTCAGGCA CAGAGTTTGA CCACGACATT
    TACTACGCCA GCCTGGAAGA CTTTAGAATG CTTGAATCCA ACCGTGGGTT CAGTACATGG
    AAGCAAGCAT TGCAAGTGGG GACACAGGGC CCATGGTGTC CGACCCTAAG CTGGGGCAAC
    CACTTGATGT ATGGGGACAC TGTCACGAGT ACTAATACTT TGCTCTCAAG CAACCCCCAA
    CTTCTTTCCA AACTAGAGTC CGGCTTCACC TTTACCGCCA CTCGCCAGGT GGCAGGCCGT
    GGCCGGGGCA ACAACGTCTG GGTCTCCCCC GCCGGCTGCT TGATAATGTC AACGGTCATC
    AACCACTCAG CGAATGTCAT TGCTTCACGC CCGTTAGGCT TCTTCAATTA CCTGGCGGCG
    ATTGCTATCG TCGAGGCGAT CAAGGGATAC GACCACGAGG ATGACATTTA CCAAAAGTTG
    GACGTCAAGA TCAAGTGGCC GAACGACATC TACGTTCGAG ACCCGAGCAA GCCCAACGAG
    CCAGCCTACG TCAAAGTTGG CGGTATCCTC GCCAACTGTT CTTACAGCGC TCCGAATTAT
    CAGGTTGTCC TCGGCATCGG ACTCAACACG AACAACGCCC AACCCACGAC CAGTCTCGAT
    GCACTTCTTA AGTGGACTGC TAGCGTGCTA CAAGGCGACA AGCCACCTCC ACCGCCGTTC
    CACATAGAAC GCCTGGTGGC CAGAATTGTC ACCTGCCTCG AGATCCTCTA CGACCACTTC
    CTGCGGAAGG GGTTCTCGGA CGAGGTGGAA GCCTTGTACT ACAAGCATTG GCTGCATACC
    AACCAGATCG TCACCCTTGA AGATGAAGAC GGCGTGAAGG CCCGCGTACT CGGTATCACC
    AAGGATCAAG GCCTTCTGGT GGCCGAGGAG GTCATGGAGG ACGGTATCGA ACTGAACTCA
    TGGAAGGGAA CGGGCACAAT GTTCCAGTTG CAGAGCGACG AGAACGGGTT CGACTACTGG
    AAGGGACTGA TCAAGAGGAA GGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B