ndc80 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	ndc80
Entrez Gene ID	2540097
Full Name	spindle pole body protein Ndc80
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022313.2	NP_596392.1	protein Ndc80

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TID3	NP_012122.3
Kluyveromyces lactis	KLLA0E13003g	XP_454536.1
Schizosaccharomyces pombe (fission yeast)	ndc80	NP_596392.1
Neurospora crassa	NCU03899	XP_957538.2
Eremothecium gossypii	AGOS_AFR281C	NP_985828.2
Magnaporthe oryzae (rice blast fungus)	MGG_01027	XP_003717915.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Purification and characterization of the fission yeast Ndc80 complex has been described.
Title: Purification and characterisation of the fission yeast Ndc80 complex.

The Ndc80 hairpin region might act as a platform for the kinetochore recruitment of Mph1.
Title: The hairpin region of Ndc80 is important for the kinetochore recruitment of Mph1/MPS1 in fission yeast.

An Ndc80 loop plays sequential roles in spindle-kinetochore attachment by connecting the Ndc80 complex to Dis1/TOG and Alp7/TACC-Alp14/TOG.
Title: The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment.

Ndc80 comprises two microtubule-interacting interfaces: the N-terminal region directly binds the microtubule lattice, while the internal loop interacts with the plus end of microtubules via Dis1/TOG.
Title: Ndc80 internal loop interacts with Dis1/TOG to ensure proper kinetochore-spindle attachment in fission yeast.

The following ndc80 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ndc80 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100760	NM_001022313.2 Latest version!	Schizosaccharomyces pombe protein Ndc80 (ndc80), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ndc80 ( NM_001022313.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596392.1 Schizosaccharomyces pombe protein Ndc80 (ndc80), mRNA.

Price & Availability↑

CloneID	OSc100760
Clone ID Related Accession (Same CDS sequence)	NM_001022313.2
Accession Version	NM_001022313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	protein Ndc80
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022313.1.

ATGCAAGATT CTTCCTCTTA CGCGAGGCGG TATTCACAGG CACCTTCTTC ATCTAATCTA 
CGAACTACTA CTTTTGGATT CAATGGACTG GGTACTTCAA GAACCAGTTT AGCACCTCAA 
AGGACTCTTG TCAATGCACG ACAGTCCATT GATCCTGACG GTTTGTCTTC TCGTGTGTTA 
ACCCCTACAA TGCGCCCAAG TTTGGCTCCT AATACCCGTC GCTCCAGCGT GCGCGTTTCC 
AATGCATCTT TTATTTCCCA AACACCTAAT ATTACTTCTA TAAAAGATCC AAGACCATTG 
TCAGACAGAA GATACCAACA AGAATGCGCT ACGCAAGTCG TCAACTATTT ATTAGAATCT 
GGATTTTCAC AGCCATTGGG TTTAAATAAT CGTTTCATGC CATCAACGAG AGAGTTTGCT 
GCCATCTTCA AACATTTGTA TAATAAGTTG GACCCAAATT TTAGATTTGG CGCACGCTAT 
GAAGAAGACG TTACTACTTG TTTAAAAGCA CTCAATTATC CGTTCCTTGA TTCCATCTCA 
CGCTCTAGGC TTGTCGCTAT AGGCTCTCCT CATGTATGGC CTGCTATATT GGGTATGCTT 
CACTGGGTTG TTTCACTAAT TCAATGCACT GAAAAGGCAG TTGCTATGGT TTATACCGTA 
GAACAAAATA GCCTTGACGA TCATCTCGTG GACAAAGTTT TGTTTGACTA CCTTGTTCGC 
ACATATCATT TGTACCTTGA TGAATCACCC GAAGAAAGTG AACCAGAGAA AGAATTAAAG 
GCTACTTTTA ATCAACAGAA CCAGGATTTG TACAATCAGA CAGAAGCTTT AAAAAGTACG 
AATGAAGAAT TGATTAATCA AATAAAATCT GCTGAGGAAC TGGACTCTGC AATCCAGGTG 
CTTGAAGAGC GCTATCGAAC TATGCAACGT GATGAAGTTA AGTTTCAGTC TGCGATGAGT 
GGAATGAAAT CAAAAATGGA ATCCAGGACT AACCTTATGA AACAACTGCA GGTAAATATT 
GAAGAAAAAG AGTCTCAGCT ACAGCTTTTA AAAGAAAAAA GGGACTCTCT GAAGTATCAA 
GTTGAAAATC AAGATATTTC TATAAGCGAA TTCGAAAAGA TGGTCTCAGA GAGGGAGCAA 
TTAGACAGAA ATTTAAACAT GATTGGAAGC AAGATTAGTG AATTAAGGAA AGAAGTGTTT 
GACACGGACT TGCTCATTCA AGCTAGCATC GATTCTTTAG AAAAGAAGGT ACAAAAATTC 
AACTCCTTGG CTTACAGAAT TGGAATTGTA CCCATTGCCG CTATTCGGTC TGCCAATAAC 
GATTTCGAAC TCGAAATTAA TCCAGAAGGC CCAAATTATA TAAATTTGGA CCTGAAGAAT 
AAAGTACGCC CATTTATTAA TGAAGTTAGA CGTTCTATTA CACTTGAGTT TCATGAAGAA 
CAAAATAAAT CCCTAAAGTT ACAAGAACAT GTTGATACCG TTAATGATTT GATAGCTGAA 
TTACAAGACG AGCTAAGAGG TATAGAATCT CGTTTAACTT CAGTATTGTC AGAATGTAAT 
ATGCTTCGTG AAACGGCCAG TGAAGAAAAA AATGCATTTG ATGCTGAATC GGATAAGTTA 
GAAAGAGAAT TACAACAGCT CAAACTTTCT TCGCATAACA GCATGCTTCA ATTAGACCAG 
CGTATACAAA GTATAAATAT CGAAGCGGAT CAAATTGCAC ATGCTTGCAT GGAGTACAAA 
AACAATATAT ATAAAGAGGT TGCATTTGTG CTTGGTGAAA TAATACATTT CAAGCTACAC 
GTTCAAGACA GTTTGGAGGA TTTGAAGATG GACTACCAAA AGGAATTGGA TGACCTATCT 
CGTTCGGAAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596392.1
CDS	84..1958
Translation	MQDSSSYARRYSQAPSSSNLRTTTFGFNGLGTSRTSLAPQRTLVNARQSIDPDGLSSRVLTPTMRPSLAPNTRRSSVRVSNASFISQTPNITSIKDPRPLSDRRYQQECATQVVNYLLESGFSQPLGLNNRFMPSTREFAAIFKHLYNKLDPNFRFGARYEEDVTTCLKALNYPFLDSISRSRLVAIGSPHVWPAILGMLHWVVSLIQCTEKAVAMVYTVEQNSLDDHLVDKVLFDYLVRTYHLYLDESPEESEPEKELKATFNQQNQDLYNQTEALKSTNEELINQIKSAEELDSAIQVLEERYRTMQRDEVKFQSAMSGMKSKMESRTNLMKQLQVNIEEKESQLQLLKEKRDSLKYQVENQDISISEFEKMVSEREQLDRNLNMIGSKISELRKEVFDTDLLIQASIDSLEKKVQKFNSLAYRIGIVPIAAIRSANNDFELEINPEGPNYINLDLKNKVRPFINEVRRSITLEFHEEQNKSLKLQEHVDTVNDLIAELQDELRGIESRLTSVLSECNMLRETASEEKNAFDAESDKLERELQQLKLSSHNSMLQLDQRIQSINIEADQIAHACMEYKNNIYKEVAFVLGEIIHFKLHVQDSLEDLKMDYQKELDDLSRSEL

Target ORF information:

RefSeq Version	NM_001022313.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe protein Ndc80 (ndc80), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022313.2

ORF Insert Sequence:

ATGCAAGATT CTTCCTCTTA CGCGAGGCGG TATTCACAGG CACCTTCTTC ATCTAATCTA 
CGAACTACTA CTTTTGGATT CAATGGACTG GGTACTTCAA GAACCAGTTT AGCACCTCAA 
AGGACTCTTG TCAATGCACG ACAGTCCATT GATCCTGACG GTTTGTCTTC TCGTGTGTTA 
ACCCCTACAA TGCGCCCAAG TTTGGCTCCT AATACCCGTC GCTCCAGCGT GCGCGTTTCC 
AATGCATCTT TTATTTCCCA AACACCTAAT ATTACTTCTA TAAAAGATCC AAGACCATTG 
TCAGACAGAA GATACCAACA AGAATGCGCT ACGCAAGTCG TCAACTATTT ATTAGAATCT 
GGATTTTCAC AGCCATTGGG TTTAAATAAT CGTTTCATGC CATCAACGAG AGAGTTTGCT 
GCCATCTTCA AACATTTGTA TAATAAGTTG GACCCAAATT TTAGATTTGG CGCACGCTAT 
GAAGAAGACG TTACTACTTG TTTAAAAGCA CTCAATTATC CGTTCCTTGA TTCCATCTCA 
CGCTCTAGGC TTGTCGCTAT AGGCTCTCCT CATGTATGGC CTGCTATATT GGGTATGCTT 
CACTGGGTTG TTTCACTAAT TCAATGCACT GAAAAGGCAG TTGCTATGGT TTATACCGTA 
GAACAAAATA GCCTTGACGA TCATCTCGTG GACAAAGTTT TGTTTGACTA CCTTGTTCGC 
ACATATCATT TGTACCTTGA TGAATCACCC GAAGAAAGTG AACCAGAGAA AGAATTAAAG 
GCTACTTTTA ATCAACAGAA CCAGGATTTG TACAATCAGA CAGAAGCTTT AAAAAGTACG 
AATGAAGAAT TGATTAATCA AATAAAATCT GCTGAGGAAC TGGACTCTGC AATCCAGGTG 
CTTGAAGAGC GCTATCGAAC TATGCAACGT GATGAAGTTA AGTTTCAGTC TGCGATGAGT 
GGAATGAAAT CAAAAATGGA ATCCAGGACT AACCTTATGA AACAACTGCA GGTAAATATT 
GAAGAAAAAG AGTCTCAGCT ACAGCTTTTA AAAGAAAAAA GGGACTCTCT GAAGTATCAA 
GTTGAAAATC AAGATATTTC TATAAGCGAA TTCGAAAAGA TGGTCTCAGA GAGGGAGCAA 
TTAGACAGAA ATTTAAACAT GATTGGAAGC AAGATTAGTG AATTAAGGAA AGAAGTGTTT 
GACACGGACT TGCTCATTCA AGCTAGCATC GATTCTTTAG AAAAGAAGGT ACAAAAATTC 
AACTCCTTGG CTTACAGAAT TGGAATTGTA CCCATTGCCG CTATTCGGTC TGCCAATAAC 
GATTTCGAAC TCGAAATTAA TCCAGAAGGC CCAAATTATA TAAATTTGGA CCTGAAGAAT 
AAAGTACGCC CATTTATTAA TGAAGTTAGA CGTTCTATTA CACTTGAGTT TCATGAAGAA 
CAAAATAAAT CCCTAAAGTT ACAAGAACAT GTTGATACCG TTAATGATTT GATAGCTGAA 
TTACAAGACG AGCTAAGAGG TATAGAATCT CGTTTAACTT CAGTATTGTC AGAATGTAAT 
ATGCTTCGTG AAACGGCCAG TGAAGAAAAA AATGCATTTG ATGCTGAATC GGATAAGTTA 
GAAAGAGAAT TACAACAGCT CAAACTTTCT TCGCATAACA GCATGCTTCA ATTAGACCAG 
CGTATACAAA GTATAAATAT CGAAGCGGAT CAAATTGCAC ATGCTTGCAT GGAGTACAAA 
AACAATATAT ATAAAGAGGT TGCATTTGTG CTTGGTGAAA TAATACATTT CAAGCTACAC 
GTTCAAGACA GTTTGGAGGA TTTGAAGATG GACTACCAAA AGGAATTGGA TGACCTATCT 
CGTTCGGAAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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ndc80 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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