ORF » Species Summary » Schizosaccharomyces pombe » brf1 cDNA ORF clone

brf1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following brf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSj01377 NM_001022185.2
Latest version!		 Schizosaccharomyces pombe 972h- transcription factor TFIIIB complex subunit Brf1 (brf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OSc100096 NM_001355799.1
Latest version!		 Schizosaccharomyces pombe transcription factor TFIIIB subunit Brf1 (brf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    brf1 ( NM_001022185.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596265.2 Schizosaccharomyces pombe 972h- transcription factor TFIIIB complex subunit Brf1 (brf1), mRNA.
    Price & Availability↑
    CloneID OSj01377
    Clone ID Related Accession (Same CDS sequence) NM_001022185.2
    Accession Version NM_001022185.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1479bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2013-04-30
    Organism Schizosaccharomyces pombe 972h-
    Product transcription factor TFIIIB complex subunit Brf1
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022185.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGATGTC CAAATTGCGG TTCAACTACT TTTGAATCTG ATACCGCTTC TGGAAATACA 
    TATTGTACAC AATGCGGTGT TGTCGTCGAG CAAGATGCAA TTGTTTCGGA AGTGACATTC 
    GGAGAGGCTT CAACTGGTGC AGCTGTTGTT CAGGGTTCTC TTGTGAGTAA TGATCAAACT 
    CATGCTCGGA CTTTTGGTGG CCCTTACAGA AATCAAGGAT CCGTAGAATC CAGAGAACTT 
    ACCATTGCAA ACGGACGTCG CCGAATTTCG GCATTGGCAA TTGCTTTAAA ACTTAACGAA 
    CGCCATATCG AAGCAGCAGT TCGCTACTTT ACACTGGCAA TTAACAACAA CTTTATAAAA 
    GGAAGGAGAT CGCAATACGT TGTTGCATCC TGTTTGTATA TTGTTTGCCG GATATCTAAG 
    ACGTCTCATA TGTTAATCGA TTTTTCTGAT ATTTTGCAAA TTAACGTTTT TAAGCTTGGG 
    TCTACTTTTC TCAAGTTGTG CCGCGTTTTA AGGCCTAATT TACCTCTTTT AGATCCGTCT 
    TTATATATCA GTAGATTTGC TTCTTTACTC GAATTTGGAC CGGAAACACA TCGTGTCGCA 
    AACGACGCTA TCAGATTGGT AGCGCGAATG AATAGAGACT GGATGCAAAT TGGTCGTCGG 
    CCAGCTGGAA TTTGTGGTGC ATGCTTACTC ATTGCAGCAA GAATGAATAA TTTTCGACGA 
    AGCGTACGAG AAGTGGTTCA TGTCGTGAAA GTTGCGGATA TCACTATTCA AAAACGCCTT 
    GATGAGTTTA AATTAACTGA GAGCGGTGAT CTTTCAATCG CCGACTTTCG AAACATTTGG 
    CTGGAAGGAC AGAGCGATCC TCCTAGCTTT ACTAAAAATC AAAAGTTTCA ACAATACGGT 
    GCTCAAAAAG TTTCAAACAT TGATCACACA CAAGAATACA TGTCCCCTAT TAAAAGGACC 
    CCGGATTTTG ATGGCAACGA AGTTAAGTCT GAAGAGCTTT CGCAAACAGT AAAAGTTGAA 
    TCTCAAGAAA CTCCTGTGCA TTTGAAGGCC GATGAAAGAG AAATTAGGAA AGAGGTTACT 
    GAAACCCTTA AGGGCGATGA ACTTAGAAAG ATTTCTCTAC AAGTTAACGT TAAATTTTCG 
    GAGGAAGAAG TTACCTTAGA AGACGTTGAT GATGATGAAA TTGAAGATAT TTTGCTTGAC 
    AAGGATGAAA TATTGACTAA AACTCAAGTT TGGATGGAGT TGAATAAAGA TTATTTAGCT 
    GAGGAAGAGG CGAAAAATTT AAAACTCCAA GAAGATTTAA AAAAAGGAAT TGTCCGACAA 
    CCTCGTAAAA GAAGACGATA TCGTCCTAGA GATAGCACTA GTGATGGAAT AGCCGATACT 
    GCTGCTGAAA GTGCAAAAGA AATGATGCAA CAAAGAGCTT TTTCAAAAAA AATCAACTAT 
    GAAGCTTTGG ATATGTTATT TGACGAAGAG CAGTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596265.2
    CDS163..1641
    Translation

    Target ORF information:

    RefSeq Version NM_001022185.2
    Organism Schizosaccharomyces pombe 972h-
    Definition Schizosaccharomyces pombe 972h- transcription factor TFIIIB complex subunit Brf1 (brf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001022185.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGATGTC CAAATTGCGG TTCAACTACT TTTGAATCTG ATACCGCTTC TGGAAATACA 
    TATTGTACAC AATGCGGTGT TGTCGTCGAG CAAGATGCAA TTGTTTCGGA AGTGACATTC 
    GGAGAGGCTT CAACTGGTGC AGCTGTTGTT CAGGGTTCTC TTGTGAGTAA TGATCAAACT 
    CATGCTCGGA CTTTTGGTGG CCCTTACAGA AATCAAGGAT CCGTAGAATC CAGAGAACTT 
    ACCATTGCAA ACGGACGTCG CCGAATTTCG GCATTGGCAA TTGCTTTAAA ACTTAACGAA 
    CGCCATATCG AAGCAGCAGT TCGCTACTTT ACACTGGCAA TTAACAACAA CTTTATAAAA 
    GGAAGGAGAT CGCAATACGT TGTTGCATCC TGTTTGTATA TTGTTTGCCG GATATCTAAG 
    ACGTCTCATA TGTTAATCGA TTTTTCTGAT ATTTTGCAAA TTAACGTTTT TAAGCTTGGG 
    TCTACTTTTC TCAAGTTGTG CCGCGTTTTA AGGCCTAATT TACCTCTTTT AGATCCGTCT 
    TTATATATCA GTAGATTTGC TTCTTTACTC GAATTTGGAC CGGAAACACA TCGTGTCGCA 
    AACGACGCTA TCAGATTGGT AGCGCGAATG AATAGAGACT GGATGCAAAT TGGTCGTCGG 
    CCAGCTGGAA TTTGTGGTGC ATGCTTACTC ATTGCAGCAA GAATGAATAA TTTTCGACGA 
    AGCGTACGAG AAGTGGTTCA TGTCGTGAAA GTTGCGGATA TCACTATTCA AAAACGCCTT 
    GATGAGTTTA AATTAACTGA GAGCGGTGAT CTTTCAATCG CCGACTTTCG AAACATTTGG 
    CTGGAAGGAC AGAGCGATCC TCCTAGCTTT ACTAAAAATC AAAAGTTTCA ACAATACGGT 
    GCTCAAAAAG TTTCAAACAT TGATCACACA CAAGAATACA TGTCCCCTAT TAAAAGGACC 
    CCGGATTTTG ATGGCAACGA AGTTAAGTCT GAAGAGCTTT CGCAAACAGT AAAAGTTGAA 
    TCTCAAGAAA CTCCTGTGCA TTTGAAGGCC GATGAAAGAG AAATTAGGAA AGAGGTTACT 
    GAAACCCTTA AGGGCGATGA ACTTAGAAAG ATTTCTCTAC AAGTTAACGT TAAATTTTCG 
    GAGGAAGAAG TTACCTTAGA AGACGTTGAT GATGATGAAA TTGAAGATAT TTTGCTTGAC 
    AAGGATGAAA TATTGACTAA AACTCAAGTT TGGATGGAGT TGAATAAAGA TTATTTAGCT 
    GAGGAAGAGG CGAAAAATTT AAAACTCCAA GAAGATTTAA AAAAAGGAAT TGTCCGACAA 
    CCTCGTAAAA GAAGACGATA TCGTCCTAGA GATAGCACTA GTGATGGAAT AGCCGATACT 
    GCTGCTGAAA GTGCAAAAGA AATGATGCAA CAAAGAGCTT TTTCAAAAAA AATCAACTAT 
    GAAGCTTTGG ATATGTTATT TGACGAAGAG CAGTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    brf1 ( NM_001355799.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342959.1 Schizosaccharomyces pombe transcription factor TFIIIB subunit Brf1 (brf1), mRNA.
    Price & Availability↑
    CloneID OSc100096
    Clone ID Related Accession (Same CDS sequence) NM_001355799.1
    Accession Version NM_001355799.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1479bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product transcription factor TFIIIB subunit Brf1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGATGTC CAAATTGCGG TTCAACTACT TTTGAATCTG ATACCGCTTC TGGAAATACA 
    TATTGTACAC AATGCGGTGT TGTCGTCGAG CAAGATGCAA TTGTTTCGGA AGTGACATTC 
    GGAGAGGCTT CAACTGGTGC AGCTGTTGTT CAGGGTTCTC TTGTGAGTAA TGATCAAACT 
    CATGCTCGGA CTTTTGGTGG CCCTTACAGA AATCAAGGAT CCGTAGAATC CAGAGAACTT 
    ACCATTGCAA ACGGACGTCG CCGAATTTCG GCATTGGCAA TTGCTTTAAA ACTTAACGAA 
    CGCCATATCG AAGCAGCAGT TCGCTACTTT ACACTGGCAA TTAACAACAA CTTTATAAAA 
    GGAAGGAGAT CGCAATACGT TGTTGCATCC TGTTTGTATA TTGTTTGCCG GATATCTAAG 
    ACGTCTCATA TGTTAATCGA TTTTTCTGAT ATTTTGCAAA TTAACGTTTT TAAGCTTGGG 
    TCTACTTTTC TCAAGTTGTG CCGCGTTTTA AGGCCTAATT TACCTCTTTT AGATCCGTCT 
    TTATATATCA GTAGATTTGC TTCTTTACTC GAATTTGGAC CGGAAACACA TCGTGTCGCA 
    AACGACGCTA TCAGATTGGT AGCGCGAATG AATAGAGACT GGATGCAAAT TGGTCGTCGG 
    CCAGCTGGAA TTTGTGGTGC ATGCTTACTC ATTGCAGCAA GAATGAATAA TTTTCGACGA 
    AGCGTACGAG AAGTGGTTCA TGTCGTGAAA GTTGCGGATA TCACTATTCA AAAACGCCTT 
    GATGAGTTTA AATTAACTGA GAGCGGTGAT CTTTCAATCG CCGACTTTCG AAACATTTGG 
    CTGGAAGGAC AGAGCGATCC TCCTAGCTTT ACTAAAAATC AAAAGTTTCA ACAATACGGT 
    GCTCAAAAAG TTTCAAACAT TGATCACACA CAAGAATACA TGTCCCCTAT TAAAAGGACC 
    CCGGATTTTG ATGGCAACGA AGTTAAGTCT GAAGAGCTTT CGCAAACAGT AAAAGTTGAA 
    TCTCAAGAAA CTCCTGTGCA TTTGAAGGCC GATGAAAGAG AAATTAGGAA AGAGGTTACT 
    GAAACCCTTA AGGGCGATGA ACTTAGAAAG ATTTCTCTAC AAGTTAACGT TAAATTTTCG 
    GAGGAAGAAG TTACCTTAGA AGACGTTGAT GATGATGAAA TTGAAGATAT TTTGCTTGAC 
    AAGGATGAAA TATTGACTAA AACTCAAGTT TGGATGGAGT TGAATAAAGA TTATTTAGCT 
    GAGGAAGAGG CGAAAAATTT AAAACTCCAA GAAGATTTAA AAAAAGGAAT TGTCCGACAA 
    CCTCGTAAAA GAAGACGATA TCGTCCTAGA GATAGCACTA GTGATGGAAT AGCCGATACT 
    GCTGCTGAAA GTGCAAAAGA AATGATGCAA CAAAGAGCTT TTTCAAAAAA AATCAACTAT 
    GAAGCTTTGG ATATGTTATT TGACGAAGAG CAGTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342959.1
    CDS163..1641
    Translation

    Target ORF information:

    RefSeq Version NM_001355799.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe transcription factor TFIIIB subunit Brf1 (brf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001355799.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGATGTC CAAATTGCGG TTCAACTACT TTTGAATCTG ATACCGCTTC TGGAAATACA 
    TATTGTACAC AATGCGGTGT TGTCGTCGAG CAAGATGCAA TTGTTTCGGA AGTGACATTC 
    GGAGAGGCTT CAACTGGTGC AGCTGTTGTT CAGGGTTCTC TTGTGAGTAA TGATCAAACT 
    CATGCTCGGA CTTTTGGTGG CCCTTACAGA AATCAAGGAT CCGTAGAATC CAGAGAACTT 
    ACCATTGCAA ACGGACGTCG CCGAATTTCG GCATTGGCAA TTGCTTTAAA ACTTAACGAA 
    CGCCATATCG AAGCAGCAGT TCGCTACTTT ACACTGGCAA TTAACAACAA CTTTATAAAA 
    GGAAGGAGAT CGCAATACGT TGTTGCATCC TGTTTGTATA TTGTTTGCCG GATATCTAAG 
    ACGTCTCATA TGTTAATCGA TTTTTCTGAT ATTTTGCAAA TTAACGTTTT TAAGCTTGGG 
    TCTACTTTTC TCAAGTTGTG CCGCGTTTTA AGGCCTAATT TACCTCTTTT AGATCCGTCT 
    TTATATATCA GTAGATTTGC TTCTTTACTC GAATTTGGAC CGGAAACACA TCGTGTCGCA 
    AACGACGCTA TCAGATTGGT AGCGCGAATG AATAGAGACT GGATGCAAAT TGGTCGTCGG 
    CCAGCTGGAA TTTGTGGTGC ATGCTTACTC ATTGCAGCAA GAATGAATAA TTTTCGACGA 
    AGCGTACGAG AAGTGGTTCA TGTCGTGAAA GTTGCGGATA TCACTATTCA AAAACGCCTT 
    GATGAGTTTA AATTAACTGA GAGCGGTGAT CTTTCAATCG CCGACTTTCG AAACATTTGG 
    CTGGAAGGAC AGAGCGATCC TCCTAGCTTT ACTAAAAATC AAAAGTTTCA ACAATACGGT 
    GCTCAAAAAG TTTCAAACAT TGATCACACA CAAGAATACA TGTCCCCTAT TAAAAGGACC 
    CCGGATTTTG ATGGCAACGA AGTTAAGTCT GAAGAGCTTT CGCAAACAGT AAAAGTTGAA 
    TCTCAAGAAA CTCCTGTGCA TTTGAAGGCC GATGAAAGAG AAATTAGGAA AGAGGTTACT 
    GAAACCCTTA AGGGCGATGA ACTTAGAAAG ATTTCTCTAC AAGTTAACGT TAAATTTTCG 
    GAGGAAGAAG TTACCTTAGA AGACGTTGAT GATGATGAAA TTGAAGATAT TTTGCTTGAC 
    AAGGATGAAA TATTGACTAA AACTCAAGTT TGGATGGAGT TGAATAAAGA TTATTTAGCT 
    GAGGAAGAGG CGAAAAATTT AAAACTCCAA GAAGATTTAA AAAAAGGAAT TGTCCGACAA 
    CCTCGTAAAA GAAGACGATA TCGTCCTAGA GATAGCACTA GTGATGGAAT AGCCGATACT 
    GCTGCTGAAA GTGCAAAAGA AATGATGCAA CAAAGAGCTT TTTCAAAAAA AATCAACTAT 
    GAAGCTTTGG ATATGTTATT TGACGAAGAG CAGTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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