Brf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brf
Entrez Gene ID	42087
Full Name	CG31256 gene product from transcript CG31256-RA
Synonyms	BRF,BRF1,Brf1,CG31256,CG4155,CG5419,Dmel\CG31256,TFIIIB,brf
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 90A3-90A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170165.2	NP_001163636.1	Brf, isoform B
NM_142359.3	NP_650616.2	Brf, isoform A

Pathways from BioSystems

Homologies

	BRF1	XP_001098066.2
Bos taurus (cattle)	BRF1	XP_005222395.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004233	XP_313145.5
Caenorhabditis elegans (roundworm)	brf-1	NP_495526.1
Saccharomyces cerevisiae (baker's yeast)	BRF1	NP_011762.1
Kluyveromyces lactis	KLLA0A05434g	XP_451240.1
Eremothecium gossypii	AGOS_AEL213W	NP_984648.1
Schizosaccharomyces pombe (fission yeast)	brf1	NP_596265.2
Xenopus tropicalis (tropical clawed frog)	brf1	NP_001182550.1
Homo sapiens (human)	BRF1	NP_001510.2
Mus musculus (house mouse)	Brf1	NP_082469.2
Gallus gallus (chicken)	BRF1	XP_004936477.1
Drosophila melanogaster (fruit fly)	Brf	NP_650616.2
Canis lupus familiaris (dog)	BRF1	XP_005623949.1
Rattus norvegicus (Norway rat)	Brf1	NP_001100231.1
Danio rerio (zebrafish)	brf1b	NP_956183.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that a portion of Brf1 is sandwiched between Bdp1 and TBP upstream of the U6 TATA box. Furthermore, Bdp1 traverses the DNA under the N-terminal stirrup of TBP to interact with the DNA (and very likely Brf1) downstream of the TATA sequence.
Title: TFIIIB subunit locations on U6 gene promoter DNA mapped by site-specific protein-DNA photo-cross-linking.

in Drosophila the alternative TRF1/BRF complex appears responsible for the initiation of all known classes of Pol III transcription
Title: Novel TRF1/BRF target genes revealed by genome-wide analysis of Drosophila Pol III transcription.

The following Brf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05215	NM_142359.3 Latest version!	Drosophila melanogaster Brf, transcript variant A (Brf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05215	NM_001170165.2 Latest version!	Drosophila melanogaster Brf, transcript variant B (Brf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brf ( NM_001170165.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163636.1 Drosophila melanogaster Brf, transcript variant B (Brf), mRNA.

Price & Availability↑

CloneID	OFa05215
Clone ID Related Accession (Same CDS sequence)	NM_001170165.2 , NM_142359.3
Accession Version	NM_001170165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Brf, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170165.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTACGG GCCTAAAATG TCGCAATTGC GGATCCAACG AGATCGAGGA AGACAATGCT 
CGCGGAGATC GAGTTTGCAT GAACTGCGGC TCGGTATTGG AGGATTCCCT CATTGTTTCC 
GAAGTGCAGT TCGAGGAAGT TGGCCATGGA GCAGCCGCAA TTGGCCAGTT TGTGTCCGCC 
GAGTCGTCGG GCGGTGCCAC CAACTATGGC TACGGCAAGT TTCAGGTGGG ATCGGGCACC 
GAATCCCGCG AGGTGACCAT CAAAAAGGCC AAGAAGGACA TCACACTACT CTGCCAGCAG 
CTGCAGCTAT CCCAGCACTA TGCAGATACT GCTCTGAATT TCTTCAAGAT GGCATTGGGT 
AGGCATCTGA CCCGCGGTAG GAAGAGCACC CACATCTACG CCGCTTGCGT GTACATGACC 
TGCAGGACAG AGGGCACCTC CCATCTCCTC ATCGACATCA GCGACGTACA GCAAATCTGT 
TCCTATGAGC TTGGTCGTAC CTACTTAAAG TTATCGCATG CCCTTTGTAT CAACATACCT 
TCTCTGGACC CGTGTTTATA CATCATGCGT TTTGCCAACC GACTGCAGTT GGGTGCCAAA 
ACGCACGAGG TTTCGATGAC GGCTCTGAGG ATTGTCCAGC GCATGAAAAA GGACTGCATG 
CACTCGGGAC GACGACCCAC TGGCCTTTGT GGAGCAGCTT TGCTGATTGC GGCCCGGATG 
CACGATTTTA GTCGCACCAT GCTGGACGTG ATAGGTGTGG TCAAGATACA CGAGTCAACG 
CTCCGAAAGC GTCTTTCGGA GTTTGCTGAA ACGCCCTCGG GAGGGCTAAC CCTGGAGGAG 
TTCATGACCG TTGACTTGGA GCGCGAACAG GATCCGCCTT CCTTTAAGGC AGCACGCAAA 
AAGGATCGTG AGAGAATCAA AGATATGGGC GAGCATGAGC TAACAGAATT GCAAAAAGAA 
ATTGATGCTC ATCTGGAAAA AGATCTAGGC AAGTACTCAA ACTCCGTTTA CCGTCAGCTG 
ACTAAGGGTA AGGGATTAAG TCCACTGAGC TCTCCAAGCA CGCCCAATAG CAGCTCGGAA 
AAGGATATTG AACTGGAGGA GTCCAGGCAG TTCATTGAAC AATCTAATGC AGAGGTAATC 
AAGGAATTGA TAGCCAAGAA CGAGGACGTG AAGAAGTCAG AACCGGGTGG TTTGGTGGCT 
GGGATTGAAG GCCTGCGGCC GGATATCGAG GCTATTTGCA GGGTGACCCA GAGCGATCTG 
GAGGACGTGG AAAAGGCTAA GCAGCCGCAA GAACAAGAAC TCATCACCGA CGATCTGAAC 
GACGACGAAC TGGATCAGTA CGTTCTGACA GAGGAGGAGT CGGTGGCAAA GTTGGAAATG 
TGGAAGAACC TCAATGCCGA GTATCTGCAA GAGCAGAAGG AACGCGACGA GCGGCTGGCC 
AAGGAACGCG AGGAGGGAAA GCCGGAGCGG AAGAAGCGAA AGCCACGCAA GAAAGTCATT 
GGTCCCTCCT CCACCGCCGG CGAGGCCATT GAAAAGATGC TGCAGGAGAA GAAGATCTCC 
AGTAAGATCA ACTACGAGAT TCTCAAGACC CTGACTGACG GCATGGGCGG GCTGACGGAT 
GACTCTCCCA CAACTAGTGC CGATACCAAA CCGAGCACTC TGGAAGAGCT GAAGCATCAG 
CCGGTTATAG TGGAAGAAGG TCCTGTTGCT TCCAAGAGTA GGGGCAATAG GGCAGCCTAC 
GATCTGCCCG GACCCAGTAG GAAACGTCCC AAACTTGAAG TGGGTCTGCC AGTCAGCAAA 
GCTGCTGATG TGGAGCAGGC GGAGACCAAG CCAGCAGTTG TGGTGGAAGC AGATGACCTG 
GACGAAGATG CTGATGATCC TGACGTCGAA CCCGAGGCAG AACCGGAGGC CACTCTTCAG 
GACATGCTCA ACACGGGCGG AGACGACGAT GAGTTCGGCT ACGGTTTTGA CGAGGAGGAG 
GAATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163636.1
CDS	176..2164
Translation	MSTGLKCRNCGSNEIEEDNARGDRVCMNCGSVLEDSLIVSEVQFEEVGHGAAAIGQFVSAESSGGATNYGYGKFQVGSGTESREVTIKKAKKDITLLCQQLQLSQHYADTALNFFKMALGRHLTRGRKSTHIYAACVYMTCRTEGTSHLLIDISDVQQICSYELGRTYLKLSHALCINIPSLDPCLYIMRFANRLQLGAKTHEVSMTALRIVQRMKKDCMHSGRRPTGLCGAALLIAARMHDFSRTMLDVIGVVKIHESTLRKRLSEFAETPSGGLTLEEFMTVDLEREQDPPSFKAARKKDRERIKDMGEHELTELQKEIDAHLEKDLGKYSNSVYRQLTKGKGLSPLSSPSTPNSSSEKDIELEESRQFIEQSNAEVIKELIAKNEDVKKSEPGGLVAGIEGLRPDIEAICRVTQSDLEDVEKAKQPQEQELITDDLNDDELDQYVLTEEESVAKLEMWKNLNAEYLQEQKERDERLAKEREEGKPERKKRKPRKKVIGPSSTAGEAIEKMLQEKKISSKINYEILKTLTDGMGGLTDDSPTTSADTKPSTLEELKHQPVIVEEGPVASKSRGNRAAYDLPGPSRKRPKLEVGLPVSKAADVEQAETKPAVVVEADDLDEDADDPDVEPEAEPEATLQDMLNTGGDDDEFGYGFDEEEEY

Target ORF information:

RefSeq Version	NM_001170165.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Brf, transcript variant B (Brf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170165.2

ORF Insert Sequence:

ATGTCTACGG GCCTAAAATG TCGCAATTGC GGATCCAACG AGATCGAGGA AGACAATGCT 
CGCGGAGATC GAGTTTGCAT GAACTGCGGC TCGGTATTGG AGGATTCCCT CATTGTTTCC 
GAAGTGCAGT TCGAGGAAGT TGGCCATGGA GCAGCCGCAA TTGGCCAGTT TGTGTCCGCC 
GAGTCGTCGG GCGGTGCCAC CAACTATGGC TACGGCAAGT TTCAGGTGGG ATCGGGCACC 
GAATCCCGCG AGGTGACCAT CAAAAAGGCC AAGAAGGACA TCACACTACT CTGCCAGCAG 
CTGCAGCTAT CCCAGCACTA TGCAGATACT GCTCTGAATT TCTTCAAGAT GGCATTGGGT 
AGGCATCTGA CCCGCGGTAG GAAGAGCACC CACATCTACG CCGCTTGCGT GTACATGACC 
TGCAGGACAG AGGGCACCTC CCATCTCCTC ATCGACATCA GCGACGTACA GCAAATCTGT 
TCCTATGAGC TTGGTCGTAC CTACTTAAAG TTATCGCATG CCCTTTGTAT CAACATACCT 
TCTCTGGACC CGTGTTTATA CATCATGCGT TTTGCCAACC GACTGCAGTT GGGTGCCAAA 
ACGCACGAGG TTTCGATGAC GGCTCTGAGG ATTGTCCAGC GCATGAAAAA GGACTGCATG 
CACTCGGGAC GACGACCCAC TGGCCTTTGT GGAGCAGCTT TGCTGATTGC GGCCCGGATG 
CACGATTTTA GTCGCACCAT GCTGGACGTG ATAGGTGTGG TCAAGATACA CGAGTCAACG 
CTCCGAAAGC GTCTTTCGGA GTTTGCTGAA ACGCCCTCGG GAGGGCTAAC CCTGGAGGAG 
TTCATGACCG TTGACTTGGA GCGCGAACAG GATCCGCCTT CCTTTAAGGC AGCACGCAAA 
AAGGATCGTG AGAGAATCAA AGATATGGGC GAGCATGAGC TAACAGAATT GCAAAAAGAA 
ATTGATGCTC ATCTGGAAAA AGATCTAGGC AAGTACTCAA ACTCCGTTTA CCGTCAGCTG 
ACTAAGGGTA AGGGATTAAG TCCACTGAGC TCTCCAAGCA CGCCCAATAG CAGCTCGGAA 
AAGGATATTG AACTGGAGGA GTCCAGGCAG TTCATTGAAC AATCTAATGC AGAGGTAATC 
AAGGAATTGA TAGCCAAGAA CGAGGACGTG AAGAAGTCAG AACCGGGTGG TTTGGTGGCT 
GGGATTGAAG GCCTGCGGCC GGATATCGAG GCTATTTGCA GGGTGACCCA GAGCGATCTG 
GAGGACGTGG AAAAGGCTAA GCAGCCGCAA GAACAAGAAC TCATCACCGA CGATCTGAAC 
GACGACGAAC TGGATCAGTA CGTTCTGACA GAGGAGGAGT CGGTGGCAAA GTTGGAAATG 
TGGAAGAACC TCAATGCCGA GTATCTGCAA GAGCAGAAGG AACGCGACGA GCGGCTGGCC 
AAGGAACGCG AGGAGGGAAA GCCGGAGCGG AAGAAGCGAA AGCCACGCAA GAAAGTCATT 
GGTCCCTCCT CCACCGCCGG CGAGGCCATT GAAAAGATGC TGCAGGAGAA GAAGATCTCC 
AGTAAGATCA ACTACGAGAT TCTCAAGACC CTGACTGACG GCATGGGCGG GCTGACGGAT 
GACTCTCCCA CAACTAGTGC CGATACCAAA CCGAGCACTC TGGAAGAGCT GAAGCATCAG 
CCGGTTATAG TGGAAGAAGG TCCTGTTGCT TCCAAGAGTA GGGGCAATAG GGCAGCCTAC 
GATCTGCCCG GACCCAGTAG GAAACGTCCC AAACTTGAAG TGGGTCTGCC AGTCAGCAAA 
GCTGCTGATG TGGAGCAGGC GGAGACCAAG CCAGCAGTTG TGGTGGAAGC AGATGACCTG 
GACGAAGATG CTGATGATCC TGACGTCGAA CCCGAGGCAG AACCGGAGGC CACTCTTCAG 
GACATGCTCA ACACGGGCGG AGACGACGAT GAGTTCGGCT ACGGTTTTGA CGAGGAGGAG 
GAATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brf ( NM_142359.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650616.2 Drosophila melanogaster Brf, transcript variant A (Brf), mRNA.

Price & Availability↑

CloneID	OFa05215
Clone ID Related Accession (Same CDS sequence)	NM_001170165.2 , NM_142359.3
Accession Version	NM_142359.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Brf, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142359.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTACGG GCCTAAAATG TCGCAATTGC GGATCCAACG AGATCGAGGA AGACAATGCT 
CGCGGAGATC GAGTTTGCAT GAACTGCGGC TCGGTATTGG AGGATTCCCT CATTGTTTCC 
GAAGTGCAGT TCGAGGAAGT TGGCCATGGA GCAGCCGCAA TTGGCCAGTT TGTGTCCGCC 
GAGTCGTCGG GCGGTGCCAC CAACTATGGC TACGGCAAGT TTCAGGTGGG ATCGGGCACC 
GAATCCCGCG AGGTGACCAT CAAAAAGGCC AAGAAGGACA TCACACTACT CTGCCAGCAG 
CTGCAGCTAT CCCAGCACTA TGCAGATACT GCTCTGAATT TCTTCAAGAT GGCATTGGGT 
AGGCATCTGA CCCGCGGTAG GAAGAGCACC CACATCTACG CCGCTTGCGT GTACATGACC 
TGCAGGACAG AGGGCACCTC CCATCTCCTC ATCGACATCA GCGACGTACA GCAAATCTGT 
TCCTATGAGC TTGGTCGTAC CTACTTAAAG TTATCGCATG CCCTTTGTAT CAACATACCT 
TCTCTGGACC CGTGTTTATA CATCATGCGT TTTGCCAACC GACTGCAGTT GGGTGCCAAA 
ACGCACGAGG TTTCGATGAC GGCTCTGAGG ATTGTCCAGC GCATGAAAAA GGACTGCATG 
CACTCGGGAC GACGACCCAC TGGCCTTTGT GGAGCAGCTT TGCTGATTGC GGCCCGGATG 
CACGATTTTA GTCGCACCAT GCTGGACGTG ATAGGTGTGG TCAAGATACA CGAGTCAACG 
CTCCGAAAGC GTCTTTCGGA GTTTGCTGAA ACGCCCTCGG GAGGGCTAAC CCTGGAGGAG 
TTCATGACCG TTGACTTGGA GCGCGAACAG GATCCGCCTT CCTTTAAGGC AGCACGCAAA 
AAGGATCGTG AGAGAATCAA AGATATGGGC GAGCATGAGC TAACAGAATT GCAAAAAGAA 
ATTGATGCTC ATCTGGAAAA AGATCTAGGC AAGTACTCAA ACTCCGTTTA CCGTCAGCTG 
ACTAAGGGTA AGGGATTAAG TCCACTGAGC TCTCCAAGCA CGCCCAATAG CAGCTCGGAA 
AAGGATATTG AACTGGAGGA GTCCAGGCAG TTCATTGAAC AATCTAATGC AGAGGTAATC 
AAGGAATTGA TAGCCAAGAA CGAGGACGTG AAGAAGTCAG AACCGGGTGG TTTGGTGGCT 
GGGATTGAAG GCCTGCGGCC GGATATCGAG GCTATTTGCA GGGTGACCCA GAGCGATCTG 
GAGGACGTGG AAAAGGCTAA GCAGCCGCAA GAACAAGAAC TCATCACCGA CGATCTGAAC 
GACGACGAAC TGGATCAGTA CGTTCTGACA GAGGAGGAGT CGGTGGCAAA GTTGGAAATG 
TGGAAGAACC TCAATGCCGA GTATCTGCAA GAGCAGAAGG AACGCGACGA GCGGCTGGCC 
AAGGAACGCG AGGAGGGAAA GCCGGAGCGG AAGAAGCGAA AGCCACGCAA GAAAGTCATT 
GGTCCCTCCT CCACCGCCGG CGAGGCCATT GAAAAGATGC TGCAGGAGAA GAAGATCTCC 
AGTAAGATCA ACTACGAGAT TCTCAAGACC CTGACTGACG GCATGGGCGG GCTGACGGAT 
GACTCTCCCA CAACTAGTGC CGATACCAAA CCGAGCACTC TGGAAGAGCT GAAGCATCAG 
CCGGTTATAG TGGAAGAAGG TCCTGTTGCT TCCAAGAGTA GGGGCAATAG GGCAGCCTAC 
GATCTGCCCG GACCCAGTAG GAAACGTCCC AAACTTGAAG TGGGTCTGCC AGTCAGCAAA 
GCTGCTGATG TGGAGCAGGC GGAGACCAAG CCAGCAGTTG TGGTGGAAGC AGATGACCTG 
GACGAAGATG CTGATGATCC TGACGTCGAA CCCGAGGCAG AACCGGAGGC CACTCTTCAG 
GACATGCTCA ACACGGGCGG AGACGACGAT GAGTTCGGCT ACGGTTTTGA CGAGGAGGAG 
GAATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650616.2
CDS	176..2164
Translation	MSTGLKCRNCGSNEIEEDNARGDRVCMNCGSVLEDSLIVSEVQFEEVGHGAAAIGQFVSAESSGGATNYGYGKFQVGSGTESREVTIKKAKKDITLLCQQLQLSQHYADTALNFFKMALGRHLTRGRKSTHIYAACVYMTCRTEGTSHLLIDISDVQQICSYELGRTYLKLSHALCINIPSLDPCLYIMRFANRLQLGAKTHEVSMTALRIVQRMKKDCMHSGRRPTGLCGAALLIAARMHDFSRTMLDVIGVVKIHESTLRKRLSEFAETPSGGLTLEEFMTVDLEREQDPPSFKAARKKDRERIKDMGEHELTELQKEIDAHLEKDLGKYSNSVYRQLTKGKGLSPLSSPSTPNSSSEKDIELEESRQFIEQSNAEVIKELIAKNEDVKKSEPGGLVAGIEGLRPDIEAICRVTQSDLEDVEKAKQPQEQELITDDLNDDELDQYVLTEEESVAKLEMWKNLNAEYLQEQKERDERLAKEREEGKPERKKRKPRKKVIGPSSTAGEAIEKMLQEKKISSKINYEILKTLTDGMGGLTDDSPTTSADTKPSTLEELKHQPVIVEEGPVASKSRGNRAAYDLPGPSRKRPKLEVGLPVSKAADVEQAETKPAVVVEADDLDEDADDPDVEPEAEPEATLQDMLNTGGDDDEFGYGFDEEEEY

Target ORF information:

RefSeq Version	NM_142359.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Brf, transcript variant A (Brf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142359.3

ORF Insert Sequence:

ATGTCTACGG GCCTAAAATG TCGCAATTGC GGATCCAACG AGATCGAGGA AGACAATGCT 
CGCGGAGATC GAGTTTGCAT GAACTGCGGC TCGGTATTGG AGGATTCCCT CATTGTTTCC 
GAAGTGCAGT TCGAGGAAGT TGGCCATGGA GCAGCCGCAA TTGGCCAGTT TGTGTCCGCC 
GAGTCGTCGG GCGGTGCCAC CAACTATGGC TACGGCAAGT TTCAGGTGGG ATCGGGCACC 
GAATCCCGCG AGGTGACCAT CAAAAAGGCC AAGAAGGACA TCACACTACT CTGCCAGCAG 
CTGCAGCTAT CCCAGCACTA TGCAGATACT GCTCTGAATT TCTTCAAGAT GGCATTGGGT 
AGGCATCTGA CCCGCGGTAG GAAGAGCACC CACATCTACG CCGCTTGCGT GTACATGACC 
TGCAGGACAG AGGGCACCTC CCATCTCCTC ATCGACATCA GCGACGTACA GCAAATCTGT 
TCCTATGAGC TTGGTCGTAC CTACTTAAAG TTATCGCATG CCCTTTGTAT CAACATACCT 
TCTCTGGACC CGTGTTTATA CATCATGCGT TTTGCCAACC GACTGCAGTT GGGTGCCAAA 
ACGCACGAGG TTTCGATGAC GGCTCTGAGG ATTGTCCAGC GCATGAAAAA GGACTGCATG 
CACTCGGGAC GACGACCCAC TGGCCTTTGT GGAGCAGCTT TGCTGATTGC GGCCCGGATG 
CACGATTTTA GTCGCACCAT GCTGGACGTG ATAGGTGTGG TCAAGATACA CGAGTCAACG 
CTCCGAAAGC GTCTTTCGGA GTTTGCTGAA ACGCCCTCGG GAGGGCTAAC CCTGGAGGAG 
TTCATGACCG TTGACTTGGA GCGCGAACAG GATCCGCCTT CCTTTAAGGC AGCACGCAAA 
AAGGATCGTG AGAGAATCAA AGATATGGGC GAGCATGAGC TAACAGAATT GCAAAAAGAA 
ATTGATGCTC ATCTGGAAAA AGATCTAGGC AAGTACTCAA ACTCCGTTTA CCGTCAGCTG 
ACTAAGGGTA AGGGATTAAG TCCACTGAGC TCTCCAAGCA CGCCCAATAG CAGCTCGGAA 
AAGGATATTG AACTGGAGGA GTCCAGGCAG TTCATTGAAC AATCTAATGC AGAGGTAATC 
AAGGAATTGA TAGCCAAGAA CGAGGACGTG AAGAAGTCAG AACCGGGTGG TTTGGTGGCT 
GGGATTGAAG GCCTGCGGCC GGATATCGAG GCTATTTGCA GGGTGACCCA GAGCGATCTG 
GAGGACGTGG AAAAGGCTAA GCAGCCGCAA GAACAAGAAC TCATCACCGA CGATCTGAAC 
GACGACGAAC TGGATCAGTA CGTTCTGACA GAGGAGGAGT CGGTGGCAAA GTTGGAAATG 
TGGAAGAACC TCAATGCCGA GTATCTGCAA GAGCAGAAGG AACGCGACGA GCGGCTGGCC 
AAGGAACGCG AGGAGGGAAA GCCGGAGCGG AAGAAGCGAA AGCCACGCAA GAAAGTCATT 
GGTCCCTCCT CCACCGCCGG CGAGGCCATT GAAAAGATGC TGCAGGAGAA GAAGATCTCC 
AGTAAGATCA ACTACGAGAT TCTCAAGACC CTGACTGACG GCATGGGCGG GCTGACGGAT 
GACTCTCCCA CAACTAGTGC CGATACCAAA CCGAGCACTC TGGAAGAGCT GAAGCATCAG 
CCGGTTATAG TGGAAGAAGG TCCTGTTGCT TCCAAGAGTA GGGGCAATAG GGCAGCCTAC 
GATCTGCCCG GACCCAGTAG GAAACGTCCC AAACTTGAAG TGGGTCTGCC AGTCAGCAAA 
GCTGCTGATG TGGAGCAGGC GGAGACCAAG CCAGCAGTTG TGGTGGAAGC AGATGACCTG 
GACGAAGATG CTGATGATCC TGACGTCGAA CCCGAGGCAG AACCGGAGGC CACTCTTCAG 
GACATGCTCA ACACGGGCGG AGACGACGAT GAGTTCGGCT ACGGTTTTGA CGAGGAGGAG 
GAATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Brf cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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