| Gene Symbol | Brf |
| Entrez Gene ID | 42087 |
| Full Name | CG31256 gene product from transcript CG31256-RA |
| Synonyms | BRF,BRF1,Brf1,CG31256,CG4155,CG5419,Dmel\CG31256,TFIIIB,brf |
| Gene Type | protein-coding |
| Organism | Drosophila melanogaster(fruit fly) |
| Genome |
ORF » Species Summary » Drosophila melanogaster » Brf cDNA ORF clone
| Gene Symbol | Brf |
| Entrez Gene ID | 42087 |
| Full Name | CG31256 gene product from transcript CG31256-RA |
| Synonyms | BRF,BRF1,Brf1,CG31256,CG4155,CG5419,Dmel\CG31256,TFIIIB,brf |
| Gene Type | protein-coding |
| Organism | Drosophila melanogaster(fruit fly) |
| Genome |
| mRNA | Protein | Name |
|---|---|---|
| NM_001170165.2 | NP_001163636.1 | Brf, isoform B |
| NM_142359.3 | NP_650616.2 | Brf, isoform A |
| BRF1 | XP_001098066.2 | |
| Bos taurus (cattle) | BRF1 | XP_005222395.1 |
| Anopheles gambiae (African malaria mosquito) | AgaP_AGAP004233 | XP_313145.5 |
| Caenorhabditis elegans (roundworm) | brf-1 | NP_495526.1 |
| Saccharomyces cerevisiae (baker's yeast) | BRF1 | NP_011762.1 |
| Kluyveromyces lactis | KLLA0A05434g | XP_451240.1 |
| Eremothecium gossypii | AGOS_AEL213W | NP_984648.1 |
| Schizosaccharomyces pombe (fission yeast) | brf1 | NP_596265.2 |
| Xenopus tropicalis (tropical clawed frog) | brf1 | NP_001182550.1 |
| Homo sapiens (human) | BRF1 | NP_001510.2 |
| Mus musculus (house mouse) | Brf1 | NP_082469.2 |
| Gallus gallus (chicken) | BRF1 | XP_004936477.1 |
| Drosophila melanogaster (fruit fly) | Brf | NP_650616.2 |
| Canis lupus familiaris (dog) | BRF1 | XP_005623949.1 |
| Rattus norvegicus (Norway rat) | Brf1 | NP_001100231.1 |
| Danio rerio (zebrafish) | brf1b | NP_956183.1 |
Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Results indicate that a portion of Brf1 is sandwiched between Bdp1 and TBP upstream of the U6 TATA box. Furthermore, Bdp1 traverses the DNA under the N-terminal stirrup of TBP to interact with the DNA (and very likely Brf1) downstream of the TATA sequence.
Title: TFIIIB subunit locations on U6 gene promoter DNA mapped by site-specific protein-DNA photo-cross-linking.
in Drosophila the alternative TRF1/BRF complex appears responsible for the initiation of all known classes of Pol III transcription
Title: Novel TRF1/BRF target genes revealed by genome-wide analysis of Drosophila Pol III transcription.
The following Brf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OFa05215 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001170165.2 , NM_142359.3 | |
| Accession Version | NM_001170165.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-04-24 | |
| Organism | Drosophila melanogaster(fruit fly) | |
| Product | Brf, isoform B | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170165.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## | |
1 | ATGTCTACGG GCCTAAAATG TCGCAATTGC GGATCCAACG AGATCGAGGA AGACAATGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001163636.1 |
| CDS | 176..2164 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001170165.2 |
1 | ATGTCTACGG GCCTAAAATG TCGCAATTGC GGATCCAACG AGATCGAGGA AGACAATGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OFa05215 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001170165.2 , NM_142359.3 | |
| Accession Version | NM_142359.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-04-24 | |
| Organism | Drosophila melanogaster(fruit fly) | |
| Product | Brf, isoform A | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142359.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## | |
1 | ATGTCTACGG GCCTAAAATG TCGCAATTGC GGATCCAACG AGATCGAGGA AGACAATGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_650616.2 |
| CDS | 176..2164 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_142359.3 |
1 | ATGTCTACGG GCCTAAAATG TCGCAATTGC GGATCCAACG AGATCGAGGA AGACAATGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data. |
Gene Model Annotations for Drosophila melanogaster: The Rule-Benders. |
Sequence finishing and mapping of Drosophila melanogaster heterochromatin. |
The Release 5.1 annotation of Drosophila melanogaster heterochromatin. |
Combined evidence annotation of transposable elements in genome sequences. |