PRP40 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PRP40
Entrez Gene ID	853857
Full Name	Prp40p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179578.3	NP_012913.3	Prp40p

Pathways from BioSystems

Homologies

	PRPF40B	XP_001101626.2
Canis lupus familiaris (dog)	PRPF40B	XP_005636926.1
Kluyveromyces lactis	KLLA0E18239g	XP_454773.1
Bos taurus (cattle)	PRPF40B	XP_002687330.3
Rattus norvegicus (Norway rat)	Prpf40b	NP_001128055.1
Xenopus tropicalis (tropical clawed frog)	prpf40b	XP_002935163.2
Pan troglodytes (chimpanzee)	PRPF40B	XP_001144883.3
Drosophila melanogaster (fruit fly)	CG3542	NP_608738.1
Homo sapiens (human)	PRPF40B	NP_001026868.2
Mus musculus (house mouse)	Prpf40b	NP_061256.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008263	XP_317209.4
Saccharomyces cerevisiae (baker's yeast)	PRP40	NP_012913.3
Eremothecium gossypii	AGOS_ADR159C	NP_984256.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Prp40 and early events in splice site definition
Title: Prp40 and early events in splice site definition.

Cloned and produced constructs corresponding to the FF2, FF3, and FF4 domains. Of these, only the constructs corresponding to FF4 gave a folded and stable sample.
Title: Solution structure of the fourth FF domain of yeast Prp40 splicing factor.

The C-terminal 2 FF domains of Prp40 are dispensable; deletion of additional FF domains is lethal; The first FF domain of Prp40 binds to U1 protein Luc7 and domains 2 and 3 bind to Snu71, another known U1 protein.
Title: The FF domains of yeast U1 snRNP protein Prp40 mediate interactions with Luc7 and Snu71.

at least two different interaction surfaces exist in FF domains that have evolved to recognize distinct binding motifs
Title: The structure of Prp40 FF1 domain and its interaction with the crn-TPR1 motif of Clf1 gives a new insight into the binding mode of FF domains.

The following PRP40 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRP40 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03046	NM_001179578.3 Latest version!	Saccharomyces cerevisiae S288C Prp40p (PRP40), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRP40 ( NM_001179578.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012913.3 Saccharomyces cerevisiae S288C Prp40p (PRP40), partial mRNA.

Price & Availability↑

CloneID	OSi03046
Clone ID Related Accession (Same CDS sequence)	NM_001179578.3
Accession Version	NM_001179578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Prp40p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Jul 30, 2012 this sequence version replaced NM_001179578.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTATTT GGAAGGAAGC TAAAGATGCA AGTGGAAGGA TATATTATTA CAATACTTTG 
ACAAAGAAAT CTACGTGGGA GAAGCCCAAG GAACTAATTT CTCAGGAGGA GCTACTTCTT 
CGAGAAAATG GCTGGAAGGC GGCCAAGACG GCAGACGGCA AAGTATACTA TTATAATCCA 
ACAACAAGAG AAACCAGCTG GACTATTCCG GCCTTCGAGA AGAAAGTAGA ACCCATCGCA 
GAACAAAAAC ATGATACAGT ATCCCATGCA CAGGTTAATG GAAATAGAAT AGCCCTTACG 
GCTGGAGAAA AACAAGAGCC GGGACGAACT ATAAACGAGG AGGAAAGCCA ATATGCTAAT 
AACTCTAAAC TGCTTAATGT CAGGAGAAGG ACTAAAGAAG AAGCAGAGAA GGAATTTATT 
ACCATGCTGA AGGAAAATCA AGTAGACTCT ACTTGGTCAT TCAGTAGAAT TATTTCAGAA 
CTGGGGACCA GAGATCCAAG GTATTGGATG GTCGATGATG ACCCCTTATG GAAGAAAGAA 
ATGTTTGAGA AATATCTTTC CAATAGATCA GCCGATCAAC TTCTTAAGGA ACACAATGAA 
ACAAGCAAAT TCAAAGAAGC CTTTCAGAAA ATGTTGCAAA ACAATTCTCA TATAAAATAT 
TACACCCGTT GGCCTACCGC AAAGAGACTA ATTGCCGACG AACCAATATA CAAACACTCC 
GTGGTCAATG AAAAGACAAA GAGACAGACC TTTCAAGATT ATATAGATAC CCTCATCGAC 
ACTCAGAAAG AATCAAAAAA AAAATTGAAA ACACAGGCCC TAAAAGAACT AAGAGAGTAT 
TTAAACGGTA TTATAACAAC ATCATCCTCT GAAACTTTCA TAACCTGGCA GCAGCTTTTA 
AATCACTATG TTTTTGATAA GAGTAAGAGA TATATGGCGA ACCGGCACTT CAAAGTCTTA 
ACCCACGAAG ATGTTTTAAA CGAGTATCTG AAAATAGTAA ATACGATTGA AAACGATCTT 
CAAAACAAAC TAAATGAGCT CCGACTGCGC AATTATACCA GAGACCGTAT TGCTAGAGAT 
AACTTTAAAA GCTTATTAAG AGAAGTGCCA ATCAAAATCA AAGCAAATAC TAGATGGTCA 
GATATTTATC CTCATATAAA GTCTGATCCG CGCTTTTTAC ATATGCTTGG AAGGAATGGC 
TCGTCCTGCC TTGATTTATT TTTAGATTTT GTTGATGAAC AAAGGATGTA CATCTTTGCA 
CAAAGATCAA TAGCCCAACA GACGTTGATA GATCAAAATT TTGAATGGAA TGATGCCGAT 
AGCGACGAGA TCACCAAGCA AAACATAGAA AAAGTTCTGG AAAATGACCG GAAATTTGAC 
AAGGTGGATA AAGAAGACAT CAGTTTGATT GTTGATGGTT TGATAAAGCA AAGAAACGAA 
AAGATACAAC AGAAACTCCA AAATGAGCGT AGGATATTGG AGCAAAAGAA GCACTATTTT 
TGGTTACTTT TGCAAAGGAC ATATACAAAA ACCGGTAAGC CCAAGCCTAG TACGTGGGAT 
TTAGCTTCCA AAGAGCTTGG CGAATCTCTT GAATACAAGG CACTAGGCGA TGAAGATAAC 
ATAAGAAGAC AAATTTTCGA GGATTTTAAG CCTGAAAGCT CTGCACCGAC TGCCGAAAGC 
GCTACTGCAA ACTTAACGTT GACCGCGTCA AAAAAGAGGC ATTTAACTCC GGCTGTGGAA 
TTGGACTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012913.3
CDS	1..1752
Translation	MSIWKEAKDASGRIYYYNTLTKKSTWEKPKELISQEELLLRENGWKAAKTADGKVYYYNPTTRETSWTIPAFEKKVEPIAEQKHDTVSHAQVNGNRIALTAGEKQEPGRTINEEESQYANNSKLLNVRRRTKEEAEKEFITMLKENQVDSTWSFSRIISELGTRDPRYWMVDDDPLWKKEMFEKYLSNRSADQLLKEHNETSKFKEAFQKMLQNNSHIKYYTRWPTAKRLIADEPIYKHSVVNEKTKRQTFQDYIDTLIDTQKESKKKLKTQALKELREYLNGIITTSSSETFITWQQLLNHYVFDKSKRYMANRHFKVLTHEDVLNEYLKIVNTIENDLQNKLNELRLRNYTRDRIARDNFKSLLREVPIKIKANTRWSDIYPHIKSDPRFLHMLGRNGSSCLDLFLDFVDEQRMYIFAQRSIAQQTLIDQNFEWNDADSDEITKQNIEKVLENDRKFDKVDKEDISLIVDGLIKQRNEKIQQKLQNERRILEQKKHYFWLLLQRTYTKTGKPKPSTWDLASKELGESLEYKALGDEDNIRRQIFEDFKPESSAPTAESATANLTLTASKKRHLTPAVELDY

Target ORF information:

RefSeq Version	NM_001179578.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Prp40p (PRP40), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179578.3

ORF Insert Sequence:

ATGTCTATTT GGAAGGAAGC TAAAGATGCA AGTGGAAGGA TATATTATTA CAATACTTTG 
ACAAAGAAAT CTACGTGGGA GAAGCCCAAG GAACTAATTT CTCAGGAGGA GCTACTTCTT 
CGAGAAAATG GCTGGAAGGC GGCCAAGACG GCAGACGGCA AAGTATACTA TTATAATCCA 
ACAACAAGAG AAACCAGCTG GACTATTCCG GCCTTCGAGA AGAAAGTAGA ACCCATCGCA 
GAACAAAAAC ATGATACAGT ATCCCATGCA CAGGTTAATG GAAATAGAAT AGCCCTTACG 
GCTGGAGAAA AACAAGAGCC GGGACGAACT ATAAACGAGG AGGAAAGCCA ATATGCTAAT 
AACTCTAAAC TGCTTAATGT CAGGAGAAGG ACTAAAGAAG AAGCAGAGAA GGAATTTATT 
ACCATGCTGA AGGAAAATCA AGTAGACTCT ACTTGGTCAT TCAGTAGAAT TATTTCAGAA 
CTGGGGACCA GAGATCCAAG GTATTGGATG GTCGATGATG ACCCCTTATG GAAGAAAGAA 
ATGTTTGAGA AATATCTTTC CAATAGATCA GCCGATCAAC TTCTTAAGGA ACACAATGAA 
ACAAGCAAAT TCAAAGAAGC CTTTCAGAAA ATGTTGCAAA ACAATTCTCA TATAAAATAT 
TACACCCGTT GGCCTACCGC AAAGAGACTA ATTGCCGACG AACCAATATA CAAACACTCC 
GTGGTCAATG AAAAGACAAA GAGACAGACC TTTCAAGATT ATATAGATAC CCTCATCGAC 
ACTCAGAAAG AATCAAAAAA AAAATTGAAA ACACAGGCCC TAAAAGAACT AAGAGAGTAT 
TTAAACGGTA TTATAACAAC ATCATCCTCT GAAACTTTCA TAACCTGGCA GCAGCTTTTA 
AATCACTATG TTTTTGATAA GAGTAAGAGA TATATGGCGA ACCGGCACTT CAAAGTCTTA 
ACCCACGAAG ATGTTTTAAA CGAGTATCTG AAAATAGTAA ATACGATTGA AAACGATCTT 
CAAAACAAAC TAAATGAGCT CCGACTGCGC AATTATACCA GAGACCGTAT TGCTAGAGAT 
AACTTTAAAA GCTTATTAAG AGAAGTGCCA ATCAAAATCA AAGCAAATAC TAGATGGTCA 
GATATTTATC CTCATATAAA GTCTGATCCG CGCTTTTTAC ATATGCTTGG AAGGAATGGC 
TCGTCCTGCC TTGATTTATT TTTAGATTTT GTTGATGAAC AAAGGATGTA CATCTTTGCA 
CAAAGATCAA TAGCCCAACA GACGTTGATA GATCAAAATT TTGAATGGAA TGATGCCGAT 
AGCGACGAGA TCACCAAGCA AAACATAGAA AAAGTTCTGG AAAATGACCG GAAATTTGAC 
AAGGTGGATA AAGAAGACAT CAGTTTGATT GTTGATGGTT TGATAAAGCA AAGAAACGAA 
AAGATACAAC AGAAACTCCA AAATGAGCGT AGGATATTGG AGCAAAAGAA GCACTATTTT 
TGGTTACTTT TGCAAAGGAC ATATACAAAA ACCGGTAAGC CCAAGCCTAG TACGTGGGAT 
TTAGCTTCCA AAGAGCTTGG CGAATCTCTT GAATACAAGG CACTAGGCGA TGAAGATAAC 
ATAAGAAGAC AAATTTTCGA GGATTTTAAG CCTGAAAGCT CTGCACCGAC TGCCGAAAGC 
GCTACTGCAA ACTTAACGTT GACCGCGTCA AAAAAGAGGC ATTTAACTCC GGCTGTGGAA 
TTGGACTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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PRP40 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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