CG3542 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG3542
Entrez Gene ID	33513
Full Name	CG3542 gene product from transcript CG3542-RA
Synonyms	Dmel\CG3542,prp40
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23C4-23C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_134894.4	NP_608738.1	uncharacterized protein

Pathways from BioSystems

Homologies

	PRPF40B	XP_001101626.2
Canis lupus familiaris (dog)	PRPF40B	XP_005636926.1
Kluyveromyces lactis	KLLA0E18239g	XP_454773.1
Bos taurus (cattle)	PRPF40B	XP_002687330.3
Rattus norvegicus (Norway rat)	Prpf40b	NP_001128055.1
Xenopus tropicalis (tropical clawed frog)	prpf40b	XP_002935163.2
Pan troglodytes (chimpanzee)	PRPF40B	XP_001144883.3
Drosophila melanogaster (fruit fly)	CG3542	NP_608738.1
Homo sapiens (human)	PRPF40B	NP_001026868.2
Mus musculus (house mouse)	Prpf40b	NP_061256.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008263	XP_317209.4
Saccharomyces cerevisiae (baker's yeast)	PRP40	NP_012913.3
Eremothecium gossypii	AGOS_ADR159C	NP_984256.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results from proteomic techniques identified CG3542 as a gene whose phosphorylation is enhanced by sex peptide treatment and provide evidence that expression of CG3542 in pickpocket neurons is required to maintain normal levels of egg-laying.
Title: A Pair of Oviduct-Born Pickpocket Neurons Important for Egg-Laying in Drosophila melanogaster.

The following CG3542 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG3542 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13589	NM_134894.4 Latest version!	Drosophila melanogaster uncharacterized protein (CG3542), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG3542 ( NM_134894.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608738.1 Drosophila melanogaster uncharacterized protein (CG3542), mRNA.

Price & Availability↑

CloneID	OFa13589
Clone ID Related Accession (Same CDS sequence)	NM_134894.4
Accession Version	NM_134894.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134894.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGTTC CTCCTAGCGT GGGCAATGGA GGAGCGCCAC CGGGAAGGGG CATAGGCTAC 
ACGCCGCCCG GTGCAATCGT GCCGCAGTTT CCACCACCCG GATTTGGGGC CCCACCCCCA 
CCGGAGCTGG CCGCCGCCTT CGGGGTGATG GCCACCAGCA CCGAGTGGAC GGAGCACAAG 
GCGCCGGACG GACGACCATA CTATTACAAT CAGAACACGA AGCAAAGCTC GTGGGAGAAG 
CCGGAGGCTC TGATGACGCC CGCCGAGCTG CTGCACAACC AGTGTCCCTG GAAGGAGTAT 
CGCTCGGATA CGGGCAAAGT GTACTACCAC AACGTGGCCA CCAAGGAGAC CTGCTGGGAG 
CCGCCGCCGG AGTACGTGGA TATGAAGGCC AAGGCGAAGG CGGAAGAAGC TGCTGCAGCC 
GCCAAAGCCG TAGCAGCCAT GACATCATCC AGCTTAGCCG GCATGGTGCC ACCTGCTGCT 
CTGGCTAGTA TTCTTCCCGC TGCCCTGCCT GTTGCCCCCC GATTACCGAC TCCTGAGATC 
CACTCGCCGC TGACACCCAG CAGCAACGAG AACTCGTCAT CAGCGATGGA TCAGGCCATG 
GCCGCCACGT TAGCGGCTAT AGAGGTGCCA CAACAAAATG CTAAGAAGGA CGACAAGTCA 
GAGAGCGCTG TGGTGTTTAA GGATAAACGC GAGGCCATCG AGTCCTTTAA AGAGCTGCTG 
CGGGACCGCA ATGTGCCATC AAATGCGAAC TGGGACCAGT GTGTGAAAAT CATATCAAAG 
GATCCGCGAT ACGCAGCCTT CAAAAATCTG AACGAGCGCA AGCAAACATT CAATGCCTAC 
AAGACGCAGA AGATTAAGGA CGAGCGCGAG GAGTCTCGCT TAAAAGCCAA GAAGGCAAAA 
GAGGACTTGG AGCAGTTCCT TATGTCCAGC GATAAGATGA ACTCGCAGAT GAAATATTTT 
CGTTGTGAAG AAGTATTTGC CGGAACGCGA ACATGGACCG CGGTGCCGGA ACCGGATCGG 
CGAGATATAT ACGAGGACTG TATATTTAAT TTAGCGAAGC GAGAGAAGGA AGAAGCGCGG 
TTGCTGAAAA AACGAAACAT GAAGGTTCTG GGCGAGCTGC TAGAGTCGAT GACTTCAATC 
AATCATGCCA CCACCTGGTC AGAGGCTCAG GTTATGCTAT TGGACAACGT AGCATTTAAG 
AATGATGTGA CTCTGCTCGG AATGGACAAG GAGGACGCCC TAATTGTCTT CGAGGAGCAC 
ATTCGCACCT TGGAAAAGGA GGAGGACGAG GAACGTGAGC GGGAAAAGAA GCGCATGAAG 
CGACAGCAGC GCAAAAACAG GGACTCCTTT CTTGCGCTGC TCGACTCCCT GCATGAAGAG 
GGAAAGCTCA CTTCCATGTC GCTGTGGGTG GAGCTGTATC CCATCATATC AGCCGATTTG 
CGATTCTCCG CTATGCTCGG CCAGAGCGGG TCTACACCCC TGGATCTTTT CAAGTTCTAT 
GTGGAAAACC TGAAAGCCCG CTTCCATGAT GAGAAAAAAA TAATAAGGGA GATCCTCAAG 
GAAAAGGCAT TTGTTGTCCA AGCGAAGACA TCCTTCGAGG ACTTTGCCAC GGTCGTGTGT 
GAAGATAAGC GGTCAGCCAG CCTGGACGCG GGAAATGTGA AGCTCACATA CAACTCCCTG 
CTGGAAAAGG CCGAGGCCAT TGAAAAGGAA CGGATGAAAG AGGAGGTGCG AAGGTTGCGG 
AAACTAGAAA ATGAAATTAA AAACGAATGG CTGGAGGCGA ACGTCTCAGT GGCCGAACCC 
TATGAGAGCG CCAAGAAGCT GGTAGAACAC CTTGAAGCAT TTGCCCTTTA TGAAAAAGAA 
ATCGGTGTGG AGAAGATCTG GGAGGACTTT GTTAAGGAAA GCGAAGATGC GTGCAGCCAT 
CATCACTCGC GATCACGAAA ATCTAAGAAA AACAAGAAGC ACAAAAAACG AGTACGGTCC 
GTCTCGAGAT CAGACATCGA GAATGAGCAT ATAGAAGTGG AGAAGTCCAA GCGAAGGCGC 
TCCAAGACAC GATCGCATTC CCTGACCTCA ATTGGCAGCA TCGAGAGCGA AAAATTGCTG 
AAGAAGAAAA AGAAGCGCAA GAACAAACTG CGAGGTTCCT CCTGCGAATC GGATGTTCCA 
GGCATTCAAT CGCCGGGAAC TCAAGCTCTA CTTCAGAACG ACTCAAACTC GCACTCACCC 
GCAAAGAAAA AGAAGAAGGA GAAGCGAGCT AAGAAGGACA AGCGACACAA CAGGCACAAT 
CGATCCGGCA CGCCCCTTAG TCCTGCCCAG TCCGTCGAGT CGTCTGGATC GCGAAACGAG 
GAGCTGACGT TAAGTGACGG CGAGCTGGAA TCGAAGCGAG CGGCTCTTCT AGCCCAACTC 
AGCGAGCAGC TCGACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608738.1
CDS	163..2583
Translation	MNVPPSVGNGGAPPGRGIGYTPPGAIVPQFPPPGFGAPPPPELAAAFGVMATSTEWTEHKAPDGRPYYYNQNTKQSSWEKPEALMTPAELLHNQCPWKEYRSDTGKVYYHNVATKETCWEPPPEYVDMKAKAKAEEAAAAAKAVAAMTSSSLAGMVPPAALASILPAALPVAPRLPTPEIHSPLTPSSNENSSSAMDQAMAATLAAIEVPQQNAKKDDKSESAVVFKDKREAIESFKELLRDRNVPSNANWDQCVKIISKDPRYAAFKNLNERKQTFNAYKTQKIKDEREESRLKAKKAKEDLEQFLMSSDKMNSQMKYFRCEEVFAGTRTWTAVPEPDRRDIYEDCIFNLAKREKEEARLLKKRNMKVLGELLESMTSINHATTWSEAQVMLLDNVAFKNDVTLLGMDKEDALIVFEEHIRTLEKEEDEEREREKKRMKRQQRKNRDSFLALLDSLHEEGKLTSMSLWVELYPIISADLRFSAMLGQSGSTPLDLFKFYVENLKARFHDEKKIIREILKEKAFVVQAKTSFEDFATVVCEDKRSASLDAGNVKLTYNSLLEKAEAIEKERMKEEVRRLRKLENEIKNEWLEANVSVAEPYESAKKLVEHLEAFALYEKEIGVEKIWEDFVKESEDACSHHHSRSRKSKKNKKHKKRVRSVSRSDIENEHIEVEKSKRRRSKTRSHSLTSIGSIESEKLLKKKKKRKNKLRGSSCESDVPGIQSPGTQALLQNDSNSHSPAKKKKKEKRAKKDKRHNRHNRSGTPLSPAQSVESSGSRNEELTLSDGELESKRAALLAQLSEQLDE

Target ORF information:

RefSeq Version	NM_134894.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG3542), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134894.4

ORF Insert Sequence:

ATGAATGTTC CTCCTAGCGT GGGCAATGGA GGAGCGCCAC CGGGAAGGGG CATAGGCTAC 
ACGCCGCCCG GTGCAATCGT GCCGCAGTTT CCACCACCCG GATTTGGGGC CCCACCCCCA 
CCGGAGCTGG CCGCCGCCTT CGGGGTGATG GCCACCAGCA CCGAGTGGAC GGAGCACAAG 
GCGCCGGACG GACGACCATA CTATTACAAT CAGAACACGA AGCAAAGCTC GTGGGAGAAG 
CCGGAGGCTC TGATGACGCC CGCCGAGCTG CTGCACAACC AGTGTCCCTG GAAGGAGTAT 
CGCTCGGATA CGGGCAAAGT GTACTACCAC AACGTGGCCA CCAAGGAGAC CTGCTGGGAG 
CCGCCGCCGG AGTACGTGGA TATGAAGGCC AAGGCGAAGG CGGAAGAAGC TGCTGCAGCC 
GCCAAAGCCG TAGCAGCCAT GACATCATCC AGCTTAGCCG GCATGGTGCC ACCTGCTGCT 
CTGGCTAGTA TTCTTCCCGC TGCCCTGCCT GTTGCCCCCC GATTACCGAC TCCTGAGATC 
CACTCGCCGC TGACACCCAG CAGCAACGAG AACTCGTCAT CAGCGATGGA TCAGGCCATG 
GCCGCCACGT TAGCGGCTAT AGAGGTGCCA CAACAAAATG CTAAGAAGGA CGACAAGTCA 
GAGAGCGCTG TGGTGTTTAA GGATAAACGC GAGGCCATCG AGTCCTTTAA AGAGCTGCTG 
CGGGACCGCA ATGTGCCATC AAATGCGAAC TGGGACCAGT GTGTGAAAAT CATATCAAAG 
GATCCGCGAT ACGCAGCCTT CAAAAATCTG AACGAGCGCA AGCAAACATT CAATGCCTAC 
AAGACGCAGA AGATTAAGGA CGAGCGCGAG GAGTCTCGCT TAAAAGCCAA GAAGGCAAAA 
GAGGACTTGG AGCAGTTCCT TATGTCCAGC GATAAGATGA ACTCGCAGAT GAAATATTTT 
CGTTGTGAAG AAGTATTTGC CGGAACGCGA ACATGGACCG CGGTGCCGGA ACCGGATCGG 
CGAGATATAT ACGAGGACTG TATATTTAAT TTAGCGAAGC GAGAGAAGGA AGAAGCGCGG 
TTGCTGAAAA AACGAAACAT GAAGGTTCTG GGCGAGCTGC TAGAGTCGAT GACTTCAATC 
AATCATGCCA CCACCTGGTC AGAGGCTCAG GTTATGCTAT TGGACAACGT AGCATTTAAG 
AATGATGTGA CTCTGCTCGG AATGGACAAG GAGGACGCCC TAATTGTCTT CGAGGAGCAC 
ATTCGCACCT TGGAAAAGGA GGAGGACGAG GAACGTGAGC GGGAAAAGAA GCGCATGAAG 
CGACAGCAGC GCAAAAACAG GGACTCCTTT CTTGCGCTGC TCGACTCCCT GCATGAAGAG 
GGAAAGCTCA CTTCCATGTC GCTGTGGGTG GAGCTGTATC CCATCATATC AGCCGATTTG 
CGATTCTCCG CTATGCTCGG CCAGAGCGGG TCTACACCCC TGGATCTTTT CAAGTTCTAT 
GTGGAAAACC TGAAAGCCCG CTTCCATGAT GAGAAAAAAA TAATAAGGGA GATCCTCAAG 
GAAAAGGCAT TTGTTGTCCA AGCGAAGACA TCCTTCGAGG ACTTTGCCAC GGTCGTGTGT 
GAAGATAAGC GGTCAGCCAG CCTGGACGCG GGAAATGTGA AGCTCACATA CAACTCCCTG 
CTGGAAAAGG CCGAGGCCAT TGAAAAGGAA CGGATGAAAG AGGAGGTGCG AAGGTTGCGG 
AAACTAGAAA ATGAAATTAA AAACGAATGG CTGGAGGCGA ACGTCTCAGT GGCCGAACCC 
TATGAGAGCG CCAAGAAGCT GGTAGAACAC CTTGAAGCAT TTGCCCTTTA TGAAAAAGAA 
ATCGGTGTGG AGAAGATCTG GGAGGACTTT GTTAAGGAAA GCGAAGATGC GTGCAGCCAT 
CATCACTCGC GATCACGAAA ATCTAAGAAA AACAAGAAGC ACAAAAAACG AGTACGGTCC 
GTCTCGAGAT CAGACATCGA GAATGAGCAT ATAGAAGTGG AGAAGTCCAA GCGAAGGCGC 
TCCAAGACAC GATCGCATTC CCTGACCTCA ATTGGCAGCA TCGAGAGCGA AAAATTGCTG 
AAGAAGAAAA AGAAGCGCAA GAACAAACTG CGAGGTTCCT CCTGCGAATC GGATGTTCCA 
GGCATTCAAT CGCCGGGAAC TCAAGCTCTA CTTCAGAACG ACTCAAACTC GCACTCACCC 
GCAAAGAAAA AGAAGAAGGA GAAGCGAGCT AAGAAGGACA AGCGACACAA CAGGCACAAT 
CGATCCGGCA CGCCCCTTAG TCCTGCCCAG TCCGTCGAGT CGTCTGGATC GCGAAACGAG 
GAGCTGACGT TAAGTGACGG CGAGCTGGAA TCGAAGCGAG CGGCTCTTCT AGCCCAACTC 
AGCGAGCAGC TCGACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG3542 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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