ORF » Species Summary » Saccharomyces cerevisiae S288C » PUS1 cDNA ORF clone

PUS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following PUS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04765 NM_001184026.1
Latest version!		 Saccharomyces cerevisiae S288C pseudouridine synthase PUS1 (PUS1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    PUS1 ( NM_001184026.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015112.1 Saccharomyces cerevisiae S288C pseudouridine synthase PUS1 (PUS1), partial mRNA.
    Price & Availability↑
    CloneID OSi04765
    Clone ID Related Accession (Same CDS sequence) NM_001184026.1
    Accession Version NM_001184026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product pseudouridine synthase PUS1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTGAAG AGAATTTGAG GCCTGCCTAT GACGATCAAG TTAATGAGGA TGTCTACAAG 
    AGAGGCGCTC AGAGCAAATT GACCAAAGCT CGTAAGGCAG ACTTCGACGA TGAAAAGGAC 
    AAAAAAAAGG ATAATGACAA ACACATAGAT AAAAGACCAA AAAGCGGTCC TCGTTTGGAT 
    GAAAATGGCA ATCCACTTCC CAAGGAGCCT CGTCTCCCAA AGAGAAAGGT GGCTGTTATG 
    GTTGGTTACT GTGGTACTGG CTACCATGGT ATGCAATATA ATCCACCAAA TCCAACCATC 
    GAGTCTGCCC TATTCAAAGC ATTTGTTGAA GCAGGTGCTA TTTCCAAGGA CAACTCCAAC 
    GATTTGAAAA AGAATGGATT CATGAGAGCT GCAAGAACTG ATAAGGGTGT CCATGCTGGA 
    GGTAATTTAA TATCATTGAA AATGATTATT GAAGATCCTG ATATCAAGCA GAAGATCAAC 
    GAAAAGTTAC CGGAAGGGAT AAGGGTATGG GATATCGAGC GTGTGAACAA GGCGTTTGAT 
    TGTAGAAAAA TGTGCAGTTC ACGTTGGTAC GAATACTTAT TACCAACTTA TTCACTCATT 
    GGCCCCAAAC CTGGTTCTAT ACTTTATAGA GACATCGAAG AAAGTAAAAC AGAATTACCT 
    GGTGTATTAG ATGAAGATTT GGAATCCAAA GAATTTTGGG AAGAGTTTAA AAAAGATGCG 
    AATGAAAAAT TTTCAACAGA AGAAATCGAA GCTATATTAG CATATGTTCC GCCCGCAAGA 
    GATGAATTTG ATATAAATGA AGAACTATAC CAGAAGGTAA AAAAGTACAA GCAATTAGAA 
    AATGCTCACC GTAGAAGATA TCGTATATCC GCGGCAAAAC TGGCCAAATT CCGTGCTTCT 
    ACAAGTCAAT ACCTTGGCGC TCATAACTTC CATAATTTTA CATTAGGCAA GGACTTCAAA 
    GAACCAAGTG CCATTCGATT TATGAAAGAT ATAAAAGTAT CTGATCCATT TGTTATTGGC 
    GATGCCCAAA CAGAATGGAT TTCCATCAAG ATTCACGGCC AGTCTTTCAT GCTACATCAA 
    ATTCGTAAGA TGGTATCAAT GGCAACCTTA ATTACCCGTT GTGGTTGCCC CGTCGAACGC 
    ATTAGTCAGG CTTACGGACA GCAGAAGATT AATATTCCGA AGGCACCTGC ATTAGGATTA 
    TTGTTAGAAG CTCCAGTATT TGAAGGTTAT AATAAAAGAC TAGAACAGTT TGGTTACAAG 
    GCGATCGATT TTTCCAAATA TCAAGACGAA GTGGATAAGT TCAAAATGAA GCATATCTAC 
    GATAAGATCT ACAAGGAAGA GGTGGACGAA AATGTTTTTA ATGCGTTCTT TAGTTATATT 
    GATAGTTTCA ACAAGGTTAC TGGAGCACAG GGAGAAGAAA CTGCCGATAA ATCGGGACCT 
    GCTGTGCAAA AGAGTATCTT CGAATTCTTG ACAGCTAAGG GTATCCCAGG TCTTACTGAT 
    GCGCCCGAGA GCAATAAGAA GATTAAGCAA AGAAAAAGAA TGGAAGAGGA GGAAGCTGCT 
    AGTAAAAAGG CTGAAATATC CAGCACCACT CAGAGCAACG AACCTGAAGT ACAACCGGAA 
    GCGGCAGCTA ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_015112.1
    CDS1..1635
    Translation

    Target ORF information:

    RefSeq Version NM_001184026.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C pseudouridine synthase PUS1 (PUS1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001184026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTGAAG AGAATTTGAG GCCTGCCTAT GACGATCAAG TTAATGAGGA TGTCTACAAG 
    AGAGGCGCTC AGAGCAAATT GACCAAAGCT CGTAAGGCAG ACTTCGACGA TGAAAAGGAC 
    AAAAAAAAGG ATAATGACAA ACACATAGAT AAAAGACCAA AAAGCGGTCC TCGTTTGGAT 
    GAAAATGGCA ATCCACTTCC CAAGGAGCCT CGTCTCCCAA AGAGAAAGGT GGCTGTTATG 
    GTTGGTTACT GTGGTACTGG CTACCATGGT ATGCAATATA ATCCACCAAA TCCAACCATC 
    GAGTCTGCCC TATTCAAAGC ATTTGTTGAA GCAGGTGCTA TTTCCAAGGA CAACTCCAAC 
    GATTTGAAAA AGAATGGATT CATGAGAGCT GCAAGAACTG ATAAGGGTGT CCATGCTGGA 
    GGTAATTTAA TATCATTGAA AATGATTATT GAAGATCCTG ATATCAAGCA GAAGATCAAC 
    GAAAAGTTAC CGGAAGGGAT AAGGGTATGG GATATCGAGC GTGTGAACAA GGCGTTTGAT 
    TGTAGAAAAA TGTGCAGTTC ACGTTGGTAC GAATACTTAT TACCAACTTA TTCACTCATT 
    GGCCCCAAAC CTGGTTCTAT ACTTTATAGA GACATCGAAG AAAGTAAAAC AGAATTACCT 
    GGTGTATTAG ATGAAGATTT GGAATCCAAA GAATTTTGGG AAGAGTTTAA AAAAGATGCG 
    AATGAAAAAT TTTCAACAGA AGAAATCGAA GCTATATTAG CATATGTTCC GCCCGCAAGA 
    GATGAATTTG ATATAAATGA AGAACTATAC CAGAAGGTAA AAAAGTACAA GCAATTAGAA 
    AATGCTCACC GTAGAAGATA TCGTATATCC GCGGCAAAAC TGGCCAAATT CCGTGCTTCT 
    ACAAGTCAAT ACCTTGGCGC TCATAACTTC CATAATTTTA CATTAGGCAA GGACTTCAAA 
    GAACCAAGTG CCATTCGATT TATGAAAGAT ATAAAAGTAT CTGATCCATT TGTTATTGGC 
    GATGCCCAAA CAGAATGGAT TTCCATCAAG ATTCACGGCC AGTCTTTCAT GCTACATCAA 
    ATTCGTAAGA TGGTATCAAT GGCAACCTTA ATTACCCGTT GTGGTTGCCC CGTCGAACGC 
    ATTAGTCAGG CTTACGGACA GCAGAAGATT AATATTCCGA AGGCACCTGC ATTAGGATTA 
    TTGTTAGAAG CTCCAGTATT TGAAGGTTAT AATAAAAGAC TAGAACAGTT TGGTTACAAG 
    GCGATCGATT TTTCCAAATA TCAAGACGAA GTGGATAAGT TCAAAATGAA GCATATCTAC 
    GATAAGATCT ACAAGGAAGA GGTGGACGAA AATGTTTTTA ATGCGTTCTT TAGTTATATT 
    GATAGTTTCA ACAAGGTTAC TGGAGCACAG GGAGAAGAAA CTGCCGATAA ATCGGGACCT 
    GCTGTGCAAA AGAGTATCTT CGAATTCTTG ACAGCTAAGG GTATCCCAGG TCTTACTGAT 
    GCGCCCGAGA GCAATAAGAA GATTAAGCAA AGAAAAAGAA TGGAAGAGGA GGAAGCTGCT 
    AGTAAAAAGG CTGAAATATC CAGCACCACT CAGAGCAACG AACCTGAAGT ACAACCGGAA 
    GCGGCAGCTA ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
    Nature387(6632 Suppl)103-5(1997 May)
    Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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