ORF » Species Summary » Schizosaccharomyces pombe » pus1 cDNA ORF clone

pus1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following pus1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pus1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99564 NM_001023437.2
Latest version!		 Schizosaccharomyces pombe tRNA pseudouridine synthase Lsp1 (pus1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    pus1 ( NM_001023437.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588446.1 Schizosaccharomyces pombe tRNA pseudouridine synthase Lsp1 (pus1), mRNA.
    Price & Availability↑
    CloneID OSc99564
    Clone ID Related Accession (Same CDS sequence) NM_001023437.2
    Accession Version NM_001023437.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product tRNA pseudouridine synthase Lsp1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023437.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGACGTG GTGGTAAACG CACTTGGTAC AATGGTGATC GTCGAGAAGC GAAAAGGAAC 
    CGACCTAATT CTATATACAA TGGAGAAGGT CGGCCAGAGA ATTTGGTAGT TGGAGAAAAA 
    AAGCCGAAAA GAAAGGTCGC CTGTCTTGTA GGTTATTGTG GTAGCGGATA TCATGGTATG 
    CAATTGAATC CTCCTAGTAA AACAATCGAA GGAGATCTTT TTGATGCTTT TGTGAAGGCT 
    GGTGCCGTTT CTTCTTATAA CGCTGATGAC CCAAAGAAAG TTGCTTTAGC AAGAGCCGCA 
    AGAACTGATA AAGGAGTTCA TGCCGCTGGA AATGTTATCA GTCTCAAACT TATAATGGAA 
    GATGAGAAGT TAATTGAAAA AGTGAACGAA CACCTCCCTC CTTCCATTCG CTTATGGGAT 
    GTGATCCGTA CTATTAATTC TTTTAACCCA AGGACTTATT GCGAATCTCG TATATATGAA 
    TATATGGTTC CAACGTATGC TTTTGTTCCA CCGAAACCTT CGTCCATACT TGGAAATTGC 
    ATTATGAAGA ATTCTCCCAT GCCTGCTGAG CCAATTAACA AGGAAAATAT AAATCAACTC 
    AGTCGTTCAT TATTTTATGA AGAAGGAAAG GAATTTTGGG ATGATTATGA TATTGCAGCC 
    AAAGAGATTT TGAGCCTTTA TGAACAGGAT CCTGAAGGCT TCGTAAATCC ATATTCTAAA 
    AGGGGAGCTG CTGCTCTTGC TAATTCGGAA AATAATAAGG GCTCTGAAGC AGGAGTTTCC 
    GCTAAAACTA ATCCAGATAT GGATTCCGAT TCCTCTGCTA TTGTAAACGA GTTTTTAAAG 
    CCTGACTCTG TTGAAGACGA ATCTGCTGGT TCCAAGATTG ATCCCAGTTA TCGTCTCGAA 
    CGTGCACTTA AGCACATTGA AGTACTTAAA TTAAAAAATT ACCGTATTTC CGCAGATAGG 
    CTGTCCGTTA TTCGAGAAAC TTTAAACCAA TATGTTGGAG TTCATAACTT TCACAATTTT 
    ACTGTGGGGC AAGCATTTCA TCAGAAGAAT AGTAATCGCG TGATCCGTTC TTTTACTGCA 
    TCTGATCCAT TCATGATTGG CGATACAGAA TGGATTTCAT GTAAAGTTCA TGGTCAATCT 
    TTTATGTTGC ACCAAATTAG AAAGATGATT GCGCTTGCTA TTTTAGTTGT ACGAACGGGT 
    TGTCCTGTTG AGCGCATACA AGATGCCTTT AAAAAAACAA AAATTAACAT ACCCAAGGGA 
    CCTGGTTTTG GACTTTTATT AGAGTCACCG TTTTTTAAAG GTTATAATGA ACATAAAGCT 
    CCAGAGAATA ATCGTGATCC TATCGACTTT ACAAAGTACG AGCAAAAAAT TACTGCTTTT 
    AAGCATGCTC ATATTTATGA TAAAATTTTT CTCGAAGAAG CTCGTAAACA AGTATTTCAT 
    TGTTTTTTGT CTTTTATTGA CAGTTATAAT GAAGAAGATT TCAGTTATTT ATCAGATATA 
    GGCATTACTG AAAAGACGCA AGAGGTTTCC TCAAAATTGC CTGATGTTCT TTCTAGCGAT 
    GAAGAAGAAG ATTCGGCTGA AAACAAGGAT GATTTAGAGG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_588446.1
    CDS40..1644
    Translation

    Target ORF information:

    RefSeq Version NM_001023437.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe tRNA pseudouridine synthase Lsp1 (pus1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001023437.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGACGTG GTGGTAAACG CACTTGGTAC AATGGTGATC GTCGAGAAGC GAAAAGGAAC 
    CGACCTAATT CTATATACAA TGGAGAAGGT CGGCCAGAGA ATTTGGTAGT TGGAGAAAAA 
    AAGCCGAAAA GAAAGGTCGC CTGTCTTGTA GGTTATTGTG GTAGCGGATA TCATGGTATG 
    CAATTGAATC CTCCTAGTAA AACAATCGAA GGAGATCTTT TTGATGCTTT TGTGAAGGCT 
    GGTGCCGTTT CTTCTTATAA CGCTGATGAC CCAAAGAAAG TTGCTTTAGC AAGAGCCGCA 
    AGAACTGATA AAGGAGTTCA TGCCGCTGGA AATGTTATCA GTCTCAAACT TATAATGGAA 
    GATGAGAAGT TAATTGAAAA AGTGAACGAA CACCTCCCTC CTTCCATTCG CTTATGGGAT 
    GTGATCCGTA CTATTAATTC TTTTAACCCA AGGACTTATT GCGAATCTCG TATATATGAA 
    TATATGGTTC CAACGTATGC TTTTGTTCCA CCGAAACCTT CGTCCATACT TGGAAATTGC 
    ATTATGAAGA ATTCTCCCAT GCCTGCTGAG CCAATTAACA AGGAAAATAT AAATCAACTC 
    AGTCGTTCAT TATTTTATGA AGAAGGAAAG GAATTTTGGG ATGATTATGA TATTGCAGCC 
    AAAGAGATTT TGAGCCTTTA TGAACAGGAT CCTGAAGGCT TCGTAAATCC ATATTCTAAA 
    AGGGGAGCTG CTGCTCTTGC TAATTCGGAA AATAATAAGG GCTCTGAAGC AGGAGTTTCC 
    GCTAAAACTA ATCCAGATAT GGATTCCGAT TCCTCTGCTA TTGTAAACGA GTTTTTAAAG 
    CCTGACTCTG TTGAAGACGA ATCTGCTGGT TCCAAGATTG ATCCCAGTTA TCGTCTCGAA 
    CGTGCACTTA AGCACATTGA AGTACTTAAA TTAAAAAATT ACCGTATTTC CGCAGATAGG 
    CTGTCCGTTA TTCGAGAAAC TTTAAACCAA TATGTTGGAG TTCATAACTT TCACAATTTT 
    ACTGTGGGGC AAGCATTTCA TCAGAAGAAT AGTAATCGCG TGATCCGTTC TTTTACTGCA 
    TCTGATCCAT TCATGATTGG CGATACAGAA TGGATTTCAT GTAAAGTTCA TGGTCAATCT 
    TTTATGTTGC ACCAAATTAG AAAGATGATT GCGCTTGCTA TTTTAGTTGT ACGAACGGGT 
    TGTCCTGTTG AGCGCATACA AGATGCCTTT AAAAAAACAA AAATTAACAT ACCCAAGGGA 
    CCTGGTTTTG GACTTTTATT AGAGTCACCG TTTTTTAAAG GTTATAATGA ACATAAAGCT 
    CCAGAGAATA ATCGTGATCC TATCGACTTT ACAAAGTACG AGCAAAAAAT TACTGCTTTT 
    AAGCATGCTC ATATTTATGA TAAAATTTTT CTCGAAGAAG CTCGTAAACA AGTATTTCAT 
    TGTTTTTTGT CTTTTATTGA CAGTTATAAT GAAGAAGATT TCAGTTATTT ATCAGATATA 
    GGCATTACTG AAAAGACGCA AGAGGTTTCC TCAAAATTGC CTGATGTTCT TTCTAGCGAT 
    GAAGAAGAAG ATTCGGCTGA AAACAAGGAT GATTTAGAGG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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