Pus1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pus1
Entrez Gene ID	304567
Full Name	pseudouridylate synthase 1
General protein information	Preferred Names pseudouridylate synthase 1 Names tRNA pseudouridine synthase A tRNA pseudouridine synthase A, mitochondrial pseudouridine synthase 1 tRNA pseudouridine(38-40) synthase tRNA pseudouridylate synthase I tRNA-uridine isomerase I
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16

mRNA and Protein(s)

mRNA	Protein	Name
XM_017598352.1	XP_017453841.1	tRNA pseudouridine synthase A, mitochondrial isoform X2
NM_001025563.1	NP_001020734.1	tRNA pseudouridine synthase A
XM_008769330.2	XP_008767552.1	tRNA pseudouridine synthase A, mitochondrial isoform X1
XM_006249547.3	XP_006249609.1	tRNA pseudouridine synthase A, mitochondrial isoform X1
XM_008769331.2	XP_008767553.1	tRNA pseudouridine synthase A, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pus1	NP_001020732.1
Neurospora crassa	NCU07528	XP_959041.1
Xenopus tropicalis (tropical clawed frog)	pus1	XP_002931922.1
Homo sapiens (human)	PUS1	NP_079491.2
Caenorhabditis elegans (roundworm)	pus-1	NP_507242.1
Schizosaccharomyces pombe (fission yeast)	pus1	NP_588446.1
Pan troglodytes (chimpanzee)	PUS1	XP_001139560.1
Bos taurus (cattle)	PUS1	XP_002694500.1
Rattus norvegicus (Norway rat)	Pus1	NP_001020734.1
Canis lupus familiaris (dog)	PUS1	XP_005636135.1
Gallus gallus (chicken)	PUS1	XP_415090.1
Saccharomyces cerevisiae (baker's yeast)	PUS1	NP_015112.1
Kluyveromyces lactis	KLLA0B02244g	XP_451631.1
Eremothecium gossypii	AGOS_AFL105C	NP_985445.2
Oryza sativa (rice)	Os02g0668400	NP_001047686.1
	LOC719360	XP_001113470.1
Danio rerio (zebrafish)	pus1	NP_001008603.1
Drosophila melanogaster (fruit fly)	CG4159	NP_001163664.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002409	XP_312543.3
Magnaporthe oryzae (rice blast fungus)	MGG_07663	XP_003711599.1
Arabidopsis thaliana (thale cress)	AT1G20370	NP_564112.1
Arabidopsis thaliana (thale cress)	AT1G76120	NP_565126.1

Gene Ontology
Bibliography

Related articles in PubMed

Steroid receptor RNA activator (SRA) modification by the human pseudouridine synthase 1 (hPus1p): RNA binding, activity, and atomic model.
Huet T, Miannay FA, Patton JR, Thore S
PloS one9(4)e94610(2014)

In human pseudouridine synthase 1 (hPus1), a C-terminal helical insert blocks tRNA from binding in the same orientation as in the Pus1 bacterial homologue TruA, consistent with their different target selectivities.
Czudnochowski N, Wang AL, Finer-Moore J, Stroud RM
Journal of molecular biology425(20)3875-87(2013 Oct)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Pseudouridine synthase 1: a site-specific synthase without strict sequence recognition requirements.
Sibert BS, Patton JR
Nucleic acids research40(5)2107-18(2012 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pus1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pus1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07498	NM_001025563.1 Latest version!	Rattus norvegicus pseudouridine synthase 1 (Pus1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa35164	XM_006249547.3 Latest version!	Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa35164	XM_008769331.2 Latest version!	Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa35164	XM_008769330.2 Latest version!	Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa57827	XM_017598352.1 Latest version!	Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pus1 ( NM_001025563.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020734.1 Rattus norvegicus pseudouridine synthase 1 (Pus1), mRNA.

Price & Availability↑

CloneID	ORa07498
Clone ID Related Accession (Same CDS sequence)	NM_001025563.1
Accession Version	NM_001025563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	tRNA pseudouridine synthase A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC098689.2. On Jul 16, 2005 this sequence version replaced XM_222267.3. ##Evidence-Data-START## Transcript exon combination :: BC161821.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTTCC CCCGCCTGTG GGCCGCACTG CTGAGAGCCT GGGGACGGTG GACGGCGCGC 
CCAGGCCCGC GCGTGCCCGG CCTGCCGCCT ATGGCTGGAA ACAAGGTGCC ACCAGCGCTG 
CCCTCACACC AGCCGGACAG GAAGGGCCGA GGTGGCTGGG TTTGGGAGGA GACGGAGCAT 
CCTGCCAAGA GGGTCAAGGG CGGTGAGGAT GAAGAGCCTC CACGCAAGCT GCCAAAGCGC 
AAAATTGTGC TGCTCATGGC CTACTCCGGC AAGGGCTACC ACGGCATGCA GAGGAATCTG 
GGGTCCTCCC AGTTCAGGAC CATCGAAGAT GACTTGGTAT CTGCCTTAGT CCAGGCAGGT 
TGTATCCCTG AAAACCATGG CACAGATATG AGGAAGATGT CTTTCCAGCG ATGTGCTCGC 
ACAGACAAGG GTGTGTCTGC AGCTGGGCAG GTGGTATCCC TAAAGGTGTG GCTAATTGAT 
GACATCCTGG ACAAGATCAA CAGCCACCTT CCATCCCATA TTCGGATTCT GGGACTGAAG 
AGGGTCACAG GTGGGTTCAA CTCCAAGAAC AAGTGTGACG CCAGGACCTA TTGCTACATG 
CTACCTACCT TTGCCTTTGC TCATAAGGAC CGGGATGTAC AGGATGAGAG CTATCGCCTA 
AGTGCAGAGA CCCTGCAGCA GGTCAACCGG CTTCTGGGCT GCTACAAAGG CACGCATAAC 
TTCCATAACT TCACATCACA GAAGGGGCCG AGAGAGCCTA GTGCACGCCG CTACATTCTG 
GAGATGTACT GCGAGGAGCC CTTTGTTCGG GAGGGCCTGG AGTTTGCTGT GATCAAGGTG 
AAGGGCCAGA GCTTCATGAT GCACCAGATC CGGAAGATGG TTGGCCTGGT GGTAGCCATT 
GTCAAGGGTT ATGCCCCTGA GAGTGTCCTG GAGCGAAGTT GGGGTGAGGA GAAGGTGGAT 
GTGCCCAAGG CACCAGGGCT TGGCTTAGTC CTGGAGAGGG TTCACTTTGA GAAGTACAAT 
CAACGCTTTG GCAGTGACGG GCTGCATGAG CCCCTGGACT GGGCACAGGA GGAAGGGAAG 
GTGACTGCTT TCAAGGAGCA ATACATCTAC CCTACCATTG TCAGCACTGA GCGGGATGAG 
CGGTCCATGG CCCAGTGGCT TAACACTTTG CCGATCCACA ACTTCAGTGG CACTGCACTT 
GGAGCGGATG ACACAGGTGC CAAGGTCCCC AGCTCCCTGG AGGGCAGTGA AGGGGATGGA 
GACACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020734.1
CDS	257..1528
Translation	MGFPRLWAALLRAWGRWTARPGPRVPGLPPMAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDESYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNFSGTALGADDTGAKVPSSLEGSEGDGDTD

Target ORF information:

RefSeq Version	NM_001025563.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pseudouridine synthase 1 (Pus1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025563.1

ORF Insert Sequence:

ATGGGCTTCC CCCGCCTGTG GGCCGCACTG CTGAGAGCCT GGGGACGGTG GACGGCGCGC 
CCAGGCCCGC GCGTGCCCGG CCTGCCGCCT ATGGCTGGAA ACAAGGTGCC ACCAGCGCTG 
CCCTCACACC AGCCGGACAG GAAGGGCCGA GGTGGCTGGG TTTGGGAGGA GACGGAGCAT 
CCTGCCAAGA GGGTCAAGGG CGGTGAGGAT GAAGAGCCTC CACGCAAGCT GCCAAAGCGC 
AAAATTGTGC TGCTCATGGC CTACTCCGGC AAGGGCTACC ACGGCATGCA GAGGAATCTG 
GGGTCCTCCC AGTTCAGGAC CATCGAAGAT GACTTGGTAT CTGCCTTAGT CCAGGCAGGT 
TGTATCCCTG AAAACCATGG CACAGATATG AGGAAGATGT CTTTCCAGCG ATGTGCTCGC 
ACAGACAAGG GTGTGTCTGC AGCTGGGCAG GTGGTATCCC TAAAGGTGTG GCTAATTGAT 
GACATCCTGG ACAAGATCAA CAGCCACCTT CCATCCCATA TTCGGATTCT GGGACTGAAG 
AGGGTCACAG GTGGGTTCAA CTCCAAGAAC AAGTGTGACG CCAGGACCTA TTGCTACATG 
CTACCTACCT TTGCCTTTGC TCATAAGGAC CGGGATGTAC AGGATGAGAG CTATCGCCTA 
AGTGCAGAGA CCCTGCAGCA GGTCAACCGG CTTCTGGGCT GCTACAAAGG CACGCATAAC 
TTCCATAACT TCACATCACA GAAGGGGCCG AGAGAGCCTA GTGCACGCCG CTACATTCTG 
GAGATGTACT GCGAGGAGCC CTTTGTTCGG GAGGGCCTGG AGTTTGCTGT GATCAAGGTG 
AAGGGCCAGA GCTTCATGAT GCACCAGATC CGGAAGATGG TTGGCCTGGT GGTAGCCATT 
GTCAAGGGTT ATGCCCCTGA GAGTGTCCTG GAGCGAAGTT GGGGTGAGGA GAAGGTGGAT 
GTGCCCAAGG CACCAGGGCT TGGCTTAGTC CTGGAGAGGG TTCACTTTGA GAAGTACAAT 
CAACGCTTTG GCAGTGACGG GCTGCATGAG CCCCTGGACT GGGCACAGGA GGAAGGGAAG 
GTGACTGCTT TCAAGGAGCA ATACATCTAC CCTACCATTG TCAGCACTGA GCGGGATGAG 
CGGTCCATGG CCCAGTGGCT TAACACTTTG CCGATCCACA ACTTCAGTGG CACTGCACTT 
GGAGCGGATG ACACAGGTGC CAAGGTCCCC AGCTCCCTGG AGGGCAGTGA AGGGGATGGA 
GACACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pus1 ( XM_008769330.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767552.1 Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa35164
Clone ID Related Accession (Same CDS sequence)	XM_008769330.2 , XM_006249547.3 , XM_008769331.2
Accession Version	XM_008769330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tRNA pseudouridine synthase A, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA ACAAGGTGCC ACCAGCGCTG CCCTCACACC AGCCGGACAG GAAGGGCCGA 
GGTGGCTGGG TTTGGGAGGA GACGGAGCAT CCTGCCAAGA GGGTCAAGGG CGGTGAGGAT 
GAAGAGCCTC CACGCAAGCT GCCAAAGCGC AAAATTGTGC TGCTCATGGC CTACTCCGGC 
AAGGGCTACC ACGGCATGCA GAGGAATCTG GGGTCCTCCC AGTTCAGGAC CATCGAAGAT 
GACTTGGTAT CTGCCTTAGT CCAGGCAGGT TGTATCCCTG AAAACCATGG CACAGATATG 
AGGAAGATGT CTTTCCAGCG ATGTGCTCGC ACAGACAAGG GTGTGTCTGC AGCTGGGCAG 
GTGGTATCCC TAAAGGTGTG GCTAATTGAT GACATCCTGG ACAAGATCAA CAGCCACCTT 
CCATCCCATA TTCGGATTCT GGGACTGAAG AGGGTCACAG GTGGGTTCAA CTCCAAGAAC 
AAGTGTGACG CCAGGACCTA TTGCTACATG CTACCTACCT TTGCCTTTGC TCATAAGGAC 
CGGGATGTAC AGGATGAGAG CTATCGCCTA AGTGCAGAGA CCCTGCAGCA GGTCAACCGG 
CTTCTGGGCT GCTACAAAGG CACGCATAAC TTCCATAACT TCACATCACA GAAGGGGCCG 
AGAGAGCCTA GTGCACGCCG CTACATTCTG GAGATGTACT GCGAGGAGCC CTTTGTTCGG 
GAGGGCCTGG AGTTTGCTGT GATCAAGGTG AAGGGCCAGA GCTTCATGAT GCACCAGATC 
CGGAAGATGG TTGGCCTGGT GGTAGCCATT GTCAAGGGTT ATGCCCCTGA GAGTGTCCTG 
GAGCGAAGTT GGGGTGAGGA GAAGGTGGAT GTGCCCAAGG CACCAGGGCT TGGCTTAGTC 
CTGGAGAGGG TTCACTTTGA GAAGTACAAT CAACGCTTTG GCAGTGACGG GCTGCATGAG 
CCCCTGGACT GGGCACAGGA GGAAGGGAAG GTGACTGCTT TCAAGGAGCA ATACATCTAC 
CCTACCATTG TCAGCACTGA GCGGGATGAG CGGTCCATGG CCCAGTGGCT TAACACTTTG 
CCGATCCACA ACTTCAGTGG CACTGCACTT GGAGCGGATG ACACAGGTGC CAAGGTCCCC 
AGCTCCCTGG AGGGCAGTGA AGGGGATGGA GACACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767552.1
CDS	495..1676
Translation	MAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDESYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNFSGTALGADDTGAKVPSSLEGSEGDGDTD

Target ORF information:

RefSeq Version	XM_008769330.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769330.2

ORF Insert Sequence:

ATGGCTGGAA ACAAGGTGCC ACCAGCGCTG CCCTCACACC AGCCGGACAG GAAGGGCCGA 
GGTGGCTGGG TTTGGGAGGA GACGGAGCAT CCTGCCAAGA GGGTCAAGGG CGGTGAGGAT 
GAAGAGCCTC CACGCAAGCT GCCAAAGCGC AAAATTGTGC TGCTCATGGC CTACTCCGGC 
AAGGGCTACC ACGGCATGCA GAGGAATCTG GGGTCCTCCC AGTTCAGGAC CATCGAAGAT 
GACTTGGTAT CTGCCTTAGT CCAGGCAGGT TGTATCCCTG AAAACCATGG CACAGATATG 
AGGAAGATGT CTTTCCAGCG ATGTGCTCGC ACAGACAAGG GTGTGTCTGC AGCTGGGCAG 
GTGGTATCCC TAAAGGTGTG GCTAATTGAT GACATCCTGG ACAAGATCAA CAGCCACCTT 
CCATCCCATA TTCGGATTCT GGGACTGAAG AGGGTCACAG GTGGGTTCAA CTCCAAGAAC 
AAGTGTGACG CCAGGACCTA TTGCTACATG CTACCTACCT TTGCCTTTGC TCATAAGGAC 
CGGGATGTAC AGGATGAGAG CTATCGCCTA AGTGCAGAGA CCCTGCAGCA GGTCAACCGG 
CTTCTGGGCT GCTACAAAGG CACGCATAAC TTCCATAACT TCACATCACA GAAGGGGCCG 
AGAGAGCCTA GTGCACGCCG CTACATTCTG GAGATGTACT GCGAGGAGCC CTTTGTTCGG 
GAGGGCCTGG AGTTTGCTGT GATCAAGGTG AAGGGCCAGA GCTTCATGAT GCACCAGATC 
CGGAAGATGG TTGGCCTGGT GGTAGCCATT GTCAAGGGTT ATGCCCCTGA GAGTGTCCTG 
GAGCGAAGTT GGGGTGAGGA GAAGGTGGAT GTGCCCAAGG CACCAGGGCT TGGCTTAGTC 
CTGGAGAGGG TTCACTTTGA GAAGTACAAT CAACGCTTTG GCAGTGACGG GCTGCATGAG 
CCCCTGGACT GGGCACAGGA GGAAGGGAAG GTGACTGCTT TCAAGGAGCA ATACATCTAC 
CCTACCATTG TCAGCACTGA GCGGGATGAG CGGTCCATGG CCCAGTGGCT TAACACTTTG 
CCGATCCACA ACTTCAGTGG CACTGCACTT GGAGCGGATG ACACAGGTGC CAAGGTCCCC 
AGCTCCCTGG AGGGCAGTGA AGGGGATGGA GACACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pus1 ( XM_006249547.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249609.1 Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35164
Clone ID Related Accession (Same CDS sequence)	XM_008769330.2 , XM_006249547.3 , XM_008769331.2
Accession Version	XM_006249547.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tRNA pseudouridine synthase A, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249547.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA ACAAGGTGCC ACCAGCGCTG CCCTCACACC AGCCGGACAG GAAGGGCCGA 
GGTGGCTGGG TTTGGGAGGA GACGGAGCAT CCTGCCAAGA GGGTCAAGGG CGGTGAGGAT 
GAAGAGCCTC CACGCAAGCT GCCAAAGCGC AAAATTGTGC TGCTCATGGC CTACTCCGGC 
AAGGGCTACC ACGGCATGCA GAGGAATCTG GGGTCCTCCC AGTTCAGGAC CATCGAAGAT 
GACTTGGTAT CTGCCTTAGT CCAGGCAGGT TGTATCCCTG AAAACCATGG CACAGATATG 
AGGAAGATGT CTTTCCAGCG ATGTGCTCGC ACAGACAAGG GTGTGTCTGC AGCTGGGCAG 
GTGGTATCCC TAAAGGTGTG GCTAATTGAT GACATCCTGG ACAAGATCAA CAGCCACCTT 
CCATCCCATA TTCGGATTCT GGGACTGAAG AGGGTCACAG GTGGGTTCAA CTCCAAGAAC 
AAGTGTGACG CCAGGACCTA TTGCTACATG CTACCTACCT TTGCCTTTGC TCATAAGGAC 
CGGGATGTAC AGGATGAGAG CTATCGCCTA AGTGCAGAGA CCCTGCAGCA GGTCAACCGG 
CTTCTGGGCT GCTACAAAGG CACGCATAAC TTCCATAACT TCACATCACA GAAGGGGCCG 
AGAGAGCCTA GTGCACGCCG CTACATTCTG GAGATGTACT GCGAGGAGCC CTTTGTTCGG 
GAGGGCCTGG AGTTTGCTGT GATCAAGGTG AAGGGCCAGA GCTTCATGAT GCACCAGATC 
CGGAAGATGG TTGGCCTGGT GGTAGCCATT GTCAAGGGTT ATGCCCCTGA GAGTGTCCTG 
GAGCGAAGTT GGGGTGAGGA GAAGGTGGAT GTGCCCAAGG CACCAGGGCT TGGCTTAGTC 
CTGGAGAGGG TTCACTTTGA GAAGTACAAT CAACGCTTTG GCAGTGACGG GCTGCATGAG 
CCCCTGGACT GGGCACAGGA GGAAGGGAAG GTGACTGCTT TCAAGGAGCA ATACATCTAC 
CCTACCATTG TCAGCACTGA GCGGGATGAG CGGTCCATGG CCCAGTGGCT TAACACTTTG 
CCGATCCACA ACTTCAGTGG CACTGCACTT GGAGCGGATG ACACAGGTGC CAAGGTCCCC 
AGCTCCCTGG AGGGCAGTGA AGGGGATGGA GACACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249609.1
CDS	487..1668
Translation	MAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDESYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNFSGTALGADDTGAKVPSSLEGSEGDGDTD

Target ORF information:

RefSeq Version	XM_006249547.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249547.3

ORF Insert Sequence:

ATGGCTGGAA ACAAGGTGCC ACCAGCGCTG CCCTCACACC AGCCGGACAG GAAGGGCCGA 
GGTGGCTGGG TTTGGGAGGA GACGGAGCAT CCTGCCAAGA GGGTCAAGGG CGGTGAGGAT 
GAAGAGCCTC CACGCAAGCT GCCAAAGCGC AAAATTGTGC TGCTCATGGC CTACTCCGGC 
AAGGGCTACC ACGGCATGCA GAGGAATCTG GGGTCCTCCC AGTTCAGGAC CATCGAAGAT 
GACTTGGTAT CTGCCTTAGT CCAGGCAGGT TGTATCCCTG AAAACCATGG CACAGATATG 
AGGAAGATGT CTTTCCAGCG ATGTGCTCGC ACAGACAAGG GTGTGTCTGC AGCTGGGCAG 
GTGGTATCCC TAAAGGTGTG GCTAATTGAT GACATCCTGG ACAAGATCAA CAGCCACCTT 
CCATCCCATA TTCGGATTCT GGGACTGAAG AGGGTCACAG GTGGGTTCAA CTCCAAGAAC 
AAGTGTGACG CCAGGACCTA TTGCTACATG CTACCTACCT TTGCCTTTGC TCATAAGGAC 
CGGGATGTAC AGGATGAGAG CTATCGCCTA AGTGCAGAGA CCCTGCAGCA GGTCAACCGG 
CTTCTGGGCT GCTACAAAGG CACGCATAAC TTCCATAACT TCACATCACA GAAGGGGCCG 
AGAGAGCCTA GTGCACGCCG CTACATTCTG GAGATGTACT GCGAGGAGCC CTTTGTTCGG 
GAGGGCCTGG AGTTTGCTGT GATCAAGGTG AAGGGCCAGA GCTTCATGAT GCACCAGATC 
CGGAAGATGG TTGGCCTGGT GGTAGCCATT GTCAAGGGTT ATGCCCCTGA GAGTGTCCTG 
GAGCGAAGTT GGGGTGAGGA GAAGGTGGAT GTGCCCAAGG CACCAGGGCT TGGCTTAGTC 
CTGGAGAGGG TTCACTTTGA GAAGTACAAT CAACGCTTTG GCAGTGACGG GCTGCATGAG 
CCCCTGGACT GGGCACAGGA GGAAGGGAAG GTGACTGCTT TCAAGGAGCA ATACATCTAC 
CCTACCATTG TCAGCACTGA GCGGGATGAG CGGTCCATGG CCCAGTGGCT TAACACTTTG 
CCGATCCACA ACTTCAGTGG CACTGCACTT GGAGCGGATG ACACAGGTGC CAAGGTCCCC 
AGCTCCCTGG AGGGCAGTGA AGGGGATGGA GACACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pus1 ( XM_008769331.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767553.1 Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X3, mRNA.

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CloneID	ORa35164
Clone ID Related Accession (Same CDS sequence)	XM_008769330.2 , XM_006249547.3 , XM_008769331.2
Accession Version	XM_008769331.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tRNA pseudouridine synthase A, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA ACAAGGTGCC ACCAGCGCTG CCCTCACACC AGCCGGACAG GAAGGGCCGA 
GGTGGCTGGG TTTGGGAGGA GACGGAGCAT CCTGCCAAGA GGGTCAAGGG CGGTGAGGAT 
GAAGAGCCTC CACGCAAGCT GCCAAAGCGC AAAATTGTGC TGCTCATGGC CTACTCCGGC 
AAGGGCTACC ACGGCATGCA GAGGAATCTG GGGTCCTCCC AGTTCAGGAC CATCGAAGAT 
GACTTGGTAT CTGCCTTAGT CCAGGCAGGT TGTATCCCTG AAAACCATGG CACAGATATG 
AGGAAGATGT CTTTCCAGCG ATGTGCTCGC ACAGACAAGG GTGTGTCTGC AGCTGGGCAG 
GTGGTATCCC TAAAGGTGTG GCTAATTGAT GACATCCTGG ACAAGATCAA CAGCCACCTT 
CCATCCCATA TTCGGATTCT GGGACTGAAG AGGGTCACAG GTGGGTTCAA CTCCAAGAAC 
AAGTGTGACG CCAGGACCTA TTGCTACATG CTACCTACCT TTGCCTTTGC TCATAAGGAC 
CGGGATGTAC AGGATGAGAG CTATCGCCTA AGTGCAGAGA CCCTGCAGCA GGTCAACCGG 
CTTCTGGGCT GCTACAAAGG CACGCATAAC TTCCATAACT TCACATCACA GAAGGGGCCG 
AGAGAGCCTA GTGCACGCCG CTACATTCTG GAGATGTACT GCGAGGAGCC CTTTGTTCGG 
GAGGGCCTGG AGTTTGCTGT GATCAAGGTG AAGGGCCAGA GCTTCATGAT GCACCAGATC 
CGGAAGATGG TTGGCCTGGT GGTAGCCATT GTCAAGGGTT ATGCCCCTGA GAGTGTCCTG 
GAGCGAAGTT GGGGTGAGGA GAAGGTGGAT GTGCCCAAGG CACCAGGGCT TGGCTTAGTC 
CTGGAGAGGG TTCACTTTGA GAAGTACAAT CAACGCTTTG GCAGTGACGG GCTGCATGAG 
CCCCTGGACT GGGCACAGGA GGAAGGGAAG GTGACTGCTT TCAAGGAGCA ATACATCTAC 
CCTACCATTG TCAGCACTGA GCGGGATGAG CGGTCCATGG CCCAGTGGCT TAACACTTTG 
CCGATCCACA ACTTCAGTGG CACTGCACTT GGAGCGGATG ACACAGGTGC CAAGGTCCCC 
AGCTCCCTGG AGGGCAGTGA AGGGGATGGA GACACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767553.1
CDS	491..1672
Translation	MAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDESYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNFSGTALGADDTGAKVPSSLEGSEGDGDTD

Target ORF information:

RefSeq Version	XM_008769331.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769331.2

ORF Insert Sequence:

ATGGCTGGAA ACAAGGTGCC ACCAGCGCTG CCCTCACACC AGCCGGACAG GAAGGGCCGA 
GGTGGCTGGG TTTGGGAGGA GACGGAGCAT CCTGCCAAGA GGGTCAAGGG CGGTGAGGAT 
GAAGAGCCTC CACGCAAGCT GCCAAAGCGC AAAATTGTGC TGCTCATGGC CTACTCCGGC 
AAGGGCTACC ACGGCATGCA GAGGAATCTG GGGTCCTCCC AGTTCAGGAC CATCGAAGAT 
GACTTGGTAT CTGCCTTAGT CCAGGCAGGT TGTATCCCTG AAAACCATGG CACAGATATG 
AGGAAGATGT CTTTCCAGCG ATGTGCTCGC ACAGACAAGG GTGTGTCTGC AGCTGGGCAG 
GTGGTATCCC TAAAGGTGTG GCTAATTGAT GACATCCTGG ACAAGATCAA CAGCCACCTT 
CCATCCCATA TTCGGATTCT GGGACTGAAG AGGGTCACAG GTGGGTTCAA CTCCAAGAAC 
AAGTGTGACG CCAGGACCTA TTGCTACATG CTACCTACCT TTGCCTTTGC TCATAAGGAC 
CGGGATGTAC AGGATGAGAG CTATCGCCTA AGTGCAGAGA CCCTGCAGCA GGTCAACCGG 
CTTCTGGGCT GCTACAAAGG CACGCATAAC TTCCATAACT TCACATCACA GAAGGGGCCG 
AGAGAGCCTA GTGCACGCCG CTACATTCTG GAGATGTACT GCGAGGAGCC CTTTGTTCGG 
GAGGGCCTGG AGTTTGCTGT GATCAAGGTG AAGGGCCAGA GCTTCATGAT GCACCAGATC 
CGGAAGATGG TTGGCCTGGT GGTAGCCATT GTCAAGGGTT ATGCCCCTGA GAGTGTCCTG 
GAGCGAAGTT GGGGTGAGGA GAAGGTGGAT GTGCCCAAGG CACCAGGGCT TGGCTTAGTC 
CTGGAGAGGG TTCACTTTGA GAAGTACAAT CAACGCTTTG GCAGTGACGG GCTGCATGAG 
CCCCTGGACT GGGCACAGGA GGAAGGGAAG GTGACTGCTT TCAAGGAGCA ATACATCTAC 
CCTACCATTG TCAGCACTGA GCGGGATGAG CGGTCCATGG CCCAGTGGCT TAACACTTTG 
CCGATCCACA ACTTCAGTGG CACTGCACTT GGAGCGGATG ACACAGGTGC CAAGGTCCCC 
AGCTCCCTGG AGGGCAGTGA AGGGGATGGA GACACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pus1 ( XM_017598352.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453841.1 Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa57827
Clone ID Related Accession (Same CDS sequence)	XM_017598352.1
Accession Version	XM_017598352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tRNA pseudouridine synthase A, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTCC CCCGCCTGTG GGCCGCACTG CTGAGAGCCT GGGGACGGTG GACGGCGCGC 
CCAGGCCCGC GCGTGCCCGG CCTGCCGCCT ATGGCTGGAA ACAAGGTGCC ACCAGCGCTG 
CCCTCACACC AGCCGGACAG GAAGGGCCGA GGTGGCTGGG TTTGGGAGGA GACGGAGCAT 
CCTGCCAAGA GGGTCAAGGG CGGTGAGGAT GAAGAGCCTC CACGCAAGCT GCCAAAGCGC 
AAAATTGTGC TGCTCATGGC CTACTCCGGC AAGGGCTACC ACGGCATGCA GGGTGTGTCT 
GCAGCTGGGC AGGTGGTATC CCTAAAGGTG TGGCTAATTG ATGACATCCT GGACAAGATC 
AACAGCCACC TTCCATCCCA TATTCGGATT CTGGGACTGA AGAGGGTCAC AGGTGGGTTC 
AACTCCAAGA ACAAGTGTGA CGCCAGGACC TATTGCTACA TGCTACCTAC CTTTGCCTTT 
GCTCATAAGG ACCGGGATGT ACAGGATGAG AGCTATCGCC TAAGTGCAGA GACCCTGCAG 
CAGGTCAACC GGCTTCTGGG CTGCTACAAA GGCACGCATA ACTTCCATAA CTTCACATCA 
CAGAAGGGGC CGAGAGAGCC TAGTGCACGC CGCTACATTC TGGAGATGTA CTGCGAGGAG 
CCCTTTGTTC GGGAGGGCCT GGAGTTTGCT GTGATCAAGG TGAAGGGCCA GAGCTTCATG 
ATGCACCAGA TCCGGAAGAT GGTTGGCCTG GTGGTAGCCA TTGTCAAGGG TTATGCCCCT 
GAGAGTGTCC TGGAGCGAAG TTGGGGTGAG GAGAAGGTGG ATGTGCCCAA GGCACCAGGG 
CTTGGCTTAG TCCTGGAGAG GGTTCACTTT GAGAAGTACA ATCAACGCTT TGGCAGTGAC 
GGGCTGCATG AGCCCCTGGA CTGGGCACAG GAGGAAGGGA AGGTGACTGC TTTCAAGGAG 
CAATACATCT ACCCTACCAT TGTCAGCACT GAGCGGGATG AGCGGTCCAT GGCCCAGTGG 
CTTAACACTT TGCCGATCCA CAACTTCAGT GGCACTGCAC TTGGAGCGGA TGACACAGGT 
GCCAAGGTCC CCAGCTCCCT GGAGGGCAGT GAAGGGGATG GAGACACTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453841.1
CDS	711..1844
Translation	MGFPRLWAALLRAWGRWTARPGPRVPGLPPMAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAYSGKGYHGMQGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDESYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNFSGTALGADDTGAKVPSSLEGSEGDGDTD

Target ORF information:

RefSeq Version	XM_017598352.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pseudouridylate synthase 1 (Pus1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598352.1

ORF Insert Sequence:

ATGGGCTTCC CCCGCCTGTG GGCCGCACTG CTGAGAGCCT GGGGACGGTG GACGGCGCGC 
CCAGGCCCGC GCGTGCCCGG CCTGCCGCCT ATGGCTGGAA ACAAGGTGCC ACCAGCGCTG 
CCCTCACACC AGCCGGACAG GAAGGGCCGA GGTGGCTGGG TTTGGGAGGA GACGGAGCAT 
CCTGCCAAGA GGGTCAAGGG CGGTGAGGAT GAAGAGCCTC CACGCAAGCT GCCAAAGCGC 
AAAATTGTGC TGCTCATGGC CTACTCCGGC AAGGGCTACC ACGGCATGCA GGGTGTGTCT 
GCAGCTGGGC AGGTGGTATC CCTAAAGGTG TGGCTAATTG ATGACATCCT GGACAAGATC 
AACAGCCACC TTCCATCCCA TATTCGGATT CTGGGACTGA AGAGGGTCAC AGGTGGGTTC 
AACTCCAAGA ACAAGTGTGA CGCCAGGACC TATTGCTACA TGCTACCTAC CTTTGCCTTT 
GCTCATAAGG ACCGGGATGT ACAGGATGAG AGCTATCGCC TAAGTGCAGA GACCCTGCAG 
CAGGTCAACC GGCTTCTGGG CTGCTACAAA GGCACGCATA ACTTCCATAA CTTCACATCA 
CAGAAGGGGC CGAGAGAGCC TAGTGCACGC CGCTACATTC TGGAGATGTA CTGCGAGGAG 
CCCTTTGTTC GGGAGGGCCT GGAGTTTGCT GTGATCAAGG TGAAGGGCCA GAGCTTCATG 
ATGCACCAGA TCCGGAAGAT GGTTGGCCTG GTGGTAGCCA TTGTCAAGGG TTATGCCCCT 
GAGAGTGTCC TGGAGCGAAG TTGGGGTGAG GAGAAGGTGG ATGTGCCCAA GGCACCAGGG 
CTTGGCTTAG TCCTGGAGAG GGTTCACTTT GAGAAGTACA ATCAACGCTT TGGCAGTGAC 
GGGCTGCATG AGCCCCTGGA CTGGGCACAG GAGGAAGGGA AGGTGACTGC TTTCAAGGAG 
CAATACATCT ACCCTACCAT TGTCAGCACT GAGCGGGATG AGCGGTCCAT GGCCCAGTGG 
CTTAACACTT TGCCGATCCA CAACTTCAGT GGCACTGCAC TTGGAGCGGA TGACACAGGT 
GCCAAGGTCC CCAGCTCCCT GGAGGGCAGT GAAGGGGATG GAGACACTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Steroid receptor RNA activator (SRA) modification by the human pseudouridine synthase 1 (hPus1p): RNA binding, activity, and atomic model.
PloS one9(4)e94610(2014)
Huet T,Miannay FA,Patton JR,Thore S

In human pseudouridine synthase 1 (hPus1), a C-terminal helical insert blocks tRNA from binding in the same orientation as in the Pus1 bacterial homologue TruA, consistent with their different target selectivities.
Journal of molecular biology425(20)3875-87(2013 Oct)
Czudnochowski N,Wang AL,Finer-Moore J,Stroud RM

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Pseudouridine synthase 1: a site-specific synthase without strict sequence recognition requirements.
Nucleic acids research40(5)2107-18(2012 Mar)
Sibert BS,Patton JR

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Pus1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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