POL2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	POL2
Entrez Gene ID	855459
Full Name	DNA polymerase epsilon catalytic subunit
Synonyms	DUN2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183100.1	NP_014137.1	DNA polymerase epsilon catalytic subunit

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

To dissect the Saccharomyces cerevisiae RNA polymerase II TL at individual-residue resolution, we quantitatively phenotyped nearly all TL single variants en masse.Our structural genetics approach incorporates structural and phenotypic data for high-resolution dissection of transcription mechanisms and their evolution, and is readily applicable to other essential yeast proteins.
Title: High-Resolution Phenotypic Landscape of the RNA Polymerase II Trigger Loop.

By detecting Pol2 and Msh2 dynamics within the same strain, we established that the mismatch recognition complex binds origins and spreads to adjacent regions with the replisome.
Title: The Eukaryotic Mismatch Recognition Complexes Track with the Replisome during DNA Synthesis.

dNTP pool levels correlate with Pol epsilon mutator severity
Title: dNTP pool levels modulate mutator phenotypes of error-prone DNA polymerase ? variants.

Study finds that wild-type Pol epsilon molecules cannot proofread errors created by proofreading-defective Pol epsilon molecules, whereas Pol delta can not only proofread errors created by proofreading-defective Pol delta molecules, but can also proofread errors created by Pol epsilon-defective molecules.
Title: Replicative DNA polymerase ? but not ? proofreads errors in Cis and in Trans.

Data suggest that DNA-directed DNA polymerase Pols alpha and delta are the primary lagging-strand replicases and Pol epsilon is primarily a leading-strand replicase.
Title: Heterogeneous polymerase fidelity and mismatch repair bias genome variation and composition.

The following POL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04387	NM_001183100.1 Latest version!	Saccharomyces cerevisiae S288C DNA polymerase epsilon catalytic subunit (POL2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POL2 ( NM_001183100.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014137.1 Saccharomyces cerevisiae S288C DNA polymerase epsilon catalytic subunit (POL2), partial mRNA.

Price & Availability↑

CloneID	OSi04387
Clone ID Related Accession (Same CDS sequence)	NM_001183100.1
Accession Version	NM_001183100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA polymerase epsilon catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATGTTTG GCAAGAAAAA AAACAACGGA GGATCTTCCA CTGCAAGATA TTCAGCTGGC 
AACAAGTACA ACACACTCTC AAATAATTAT GCGCTTAGCG CGCAACAGCT CTTAAATGCT 
AGTAAGATCG ATGACATCGA TTCGATGATG GGATTTGAAA GATACGTACC GCCGCAATAC 
AATGGCAGGT TTGATGCGAA GGATATAGAT CAGATTCCAG GCCGCGTAGG GTGGCTGACG 
AACATGCACG CAACGCTGGT CTCTCAGGAA ACCTTATCCA GTGGTAGTAA TGGCGGCGGC 
AATTCGAATG ACGGAGAACG TGTAACGACC AACCAAGGTA TTTCCGGAGT TGACTTCTAC 
TTTTTAGATG AAGAGGGTGG GAGCTTCAAG TCGACAGTTG TCTATGACCC ATACTTCTTT 
ATTGCGTGTA ACGATGAATC AAGAGTAAAT GATGTGGAGG AACTAGTGAA AAAATATCTG 
GAATCTTGTC TCAAAAGCTT ACAAATCATT AGAAAGGAAG ATCTTACCAT GGACAATCAC 
CTTTTAGGGC TGCAGAAGAC ACTTATTAAG TTATCATTTG TAAATTCCAA TCAGTTATTC 
GAGGCCAGGA AACTCCTGAG GCCAATCTTG CAGGATAATG CCAATAATAA TGTGCAAAGA 
AATATATATA ACGTTGCTGC AAATGGCTCG GAAAAAGTTG ACGCCAAACA TCTGATCGAA 
GATATCAGGG AATATGATGT GCCGTATCAT GTCCGAGTAT CTATAGACAA GGACATTAGA 
GTCGGTAAAT GGTATAAGGT AACTCAACAG GGATTCATTG AAGATACTAG GAAAATTGCA 
TTTGCCGACC CTGTGGTAAT GGCATTTGAT ATAGAAACCA CGAAGCCGCC TTTAAAATTC 
CCGGATTCCG CCGTAGATCA AATAATGATG ATTTCGTATA TGATCGATGG GGAAGGTTTT 
TTGATAACAA ATAGGGAGAT AATCTCTGAG GATATTGAAG ACTTTGAGTA TACACCGAAA 
CCGGAGTATC CTGGTTTTTT CACCATATTT AACGAAAACG ATGAAGTGGC GCTTCTACAA 
AGGTTTTTTG AACATATAAG AGATGTACGA CCCACTGTTA TATCCACCTT CAATGGTGAC 
TTTTTCGATT GGCCTTTTAT ACATAACAGA AGTAAGATTC ACGGCTTGGA CATGTTCGAT 
GAAATTGGTT TCGCTCCAGA TGCTGAAGGT GAGTACAAGT CCTCATACTG CTCTCACATG 
GATTGTTTCC GTTGGGTGAA GCGTGATTCT TATTTACCAC AAGGTTCCCA GGGTTTAAAA 
GCTGTTACTC AATCTAAGCT AGGTTATAAC CCAATTGAAC TGGATCCCGA ATTAATGACG 
CCGTATGCAT TTGAAAAGCC ACAGCACCTT TCCGAATATT CTGTTTCCGA TGCAGTCGCT 
ACGTATTACC TTTACATGAA ATATGTTCAT CCTTTTATCT TTTCCCTTTG TACTATTATT 
CCTTTGAACC CGGATGAAAC ATTGAGAAAG GGTACCGGTA CTTTGTGTGA AATGTTGTTG 
ATGGTTCAAG CTTATCAACA TAATATTCTT CTACCAAATA AGCATACAGA TCCCATTGAG 
AGGTTCTATG ATGGACATCT TCTAGAATCC GAGACTTACG TGGGTGGACA TGTGGAGTCA 
TTAGAAGCTG GTGTTTTTAG GAGTGATTTG AAGAATGAAT TCAAGATAGA TCCTTCTGCC 
ATTGATGAAT TATTACAAGA ATTACCAGAA GCTTTGAAAT TTAGTGTGGA AGTTGAAAAT 
AAGTCCAGTG TAGATAAAGT AACGAATTTT GAGGAAATAA AAAACCAGAT AACGCAGAAA 
TTATTAGAGT TGAAGGAAAA CAATATAAGA AACGAACTAC CTTTGATCTA TCATGTAGAT 
GTCGCCTCTA TGTACCCAAA CATCATGACT ACAAATAGAC TACAACCAGA TAGTATCAAA 
GCAGAGCGCG ATTGTGCTAG TTGCGATTTT AATAGACCCG GAAAAACCTG TGCAAGAAAG 
TTAAAATGGG CTTGGAGAGG AGAATTCTTT CCCAGTAAGA TGGATGAGTA TAACATGATC 
AAGCGTGCAT TACAAAATGA GACTTTTCCC AACAAAAACA AGTTTTCTAA AAAGAAAGTT 
TTGACATTTG ATGAACTAAG TTACGCAGAC CAAGTTATCC ACATAAAAAA ACGTTTAACT 
GAATATTCAA GGAAAGTTTA TCATAGGGTT AAAGTATCAG AAATTGTCGA ACGAGAAGCC 
ATTGTCTGCC AAAGAGAAAA TCCATTCTAC GTCGATACCG TGAAATCCTT TCGTGATAGG 
CGTTACGAAT TCAAAGGTTT AGCCAAGACT TGGAAGGGAA ATCTGTCCAA AATTGACCCA 
TCTGATAAGC ATGCGAGAGA CGAGGCCAAA AAGATGATTG TGCTTTATGA CTCATTACAA 
TTAGCTCACA AAGTTATTTT GAATTCGTTT TATGGGTATG TTATGAGGAA AGGCTCTCGT 
TGGTATTCCA TGGAAATGGC GGGGATTACG TGTTTAACAG GTGCCACGAT CATTCAAATG 
GCGAGAGCTT TAGTAGAAAG GGTAGGAAGA CCATTAGAAT TAGATACTGA TGGTATTTGG 
TGTATCTTAC CAAAATCTTT CCCTGAAACT TACTTTTTTA CATTAGAAAA TGGTAAAAAG 
CTTTATCTCT CCTACCCATG TTCCATGCTG AATTACAGAG TTCACCAAAA GTTTACGAAT 
CACCAATACC AAGAATTAAA AGACCCATTG AACTATATAT ATGAGACGCA CAGTGAAAAC 
ACGATTTTTT TCGAAGTTGA CGGACCATAT AAGGCCATGA TTTTGCCTAG TTCCAAGGAA 
GAAGGAAAAG GTATAAAGAA AAGATATGCT GTCTTCAATG AAGACGGCTC ACTTGCTGAA 
CTGAAAGGTT TTGAATTGAA GAGGCGTGGT GAATTACAAC TAATAAAAAA TTTTCAAAGT 
GATATTTTCA AGGTCTTTTT GGAAGGTGAT ACATTAGAAG GATGTTACAG TGCTGTAGCA 
AGCGTATGTA ACCGTTGGTT AGATGTTCTT GATTCACATG GTCTTATGTT AGAAGATGAA 
GACTTGGTCA GTTTGATTTG TGAAAATAGA AGTATGTCAA AAACTTTAAA GGAATATGAA 
GGGCAAAAAT CTACTTCTAT TACGACGGCA AGGAGATTGG GGGATTTTTT GGGTGAAGAT 
ATGGTAAAAG ATAAAGGTCT ACAATGTAAA TATATTATTA GTTCAAAACC TTTCAATGCA 
CCTGTTACTG AACGAGCCAT TCCAGTCGCA ATATTTTCAG CGGACATTCC CATCAAAAGG 
TCTTTTCTGA GGCGATGGAC ATTAGATCCA TCTTTGGAAG ATCTGGATAT CAGAACCATA 
ATCGATTGGG GTTATTATAG AGAAAGACTT GGATCTGCTA TACAAAAGAT AATTACTATT 
CCAGCAGCAT TACAAGGGGT TTCCAATCCT GTTCCAAGGG TTGAACATCC AGATTGGCTA 
AAAAGAAAAA TCGCTACAAA GGAGGATAAG TTTAAGCAGA CTTCACTAAC CAAATTTTTT 
TCGAAGACAA AGAATGTACC AACAATGGGC AAGATAAAAG ATATCGAGGA TTTGTTTGAA 
CCAACTGTAG AAGAAGATAA CGCCAAAATT AAAATTGCAA GAACTACTAA AAAGAAAGCC 
GTATCCAAGA GGAAAAGAAA TCAGCTTACA AATGAAGAAG ATCCACTAGT ATTGCCCTCG 
GAGATTCCTT CCATGGACGA GGACTATGTT GGGTGGCTAA ATTATCAAAA AATTAAATGG 
AAAATCCAAG CAAGAGATAG AAAGCGTCGA GACCAATTAT TTGGTAATAC AAACAGCTCC 
CGTGAAAGAA GTGCACTAGG AAGTATGATT AGGAAGCAAG CTGAATCATA TGCGAACTCC 
ACTTGGGAGG TCTTACAATA CAAGGATTCC GGTGAGCCAG GGGTTTTGGA AGTATTTGTA 
ACAATTAATG GCAAAGTCCA GAACATCACC TTCCATATAC CAAAAACTAT TTATATGAAA 
TTCAAATCTC AAACAATGCC GCTACAAAAG ATTAAGAATT GCCTTATTGA AAAATCTTCT 
GCATCGTTAC CAAATAATCC CAAAACGTCT AATCCAGCAG GCGGTCAGCT ATTCAAAATT 
ACTCTACCGG AATCTGTCTT TCTGGAAGAA AAGGAAAACT GCACTAGTAT CTTCAACGAT 
GAAAATGTAC TTGGTGTATT TGAGGGCACT ATCACTCCTC ATCAAAGAGC GATCATGGAT 
TTGGGAGCTT CGGTAACGTT CCGCTCAAAA GCAATGGGTG CGTTAGGCAA GGGAATACAG 
CAGGGTTTTG AAATGAAGGA TCTTTCAATG GCGGAAAATG AAAGGTATCT GAGTGGATTT 
TCAATGGACA TTGGCTATTT ACTACATTTC CCAACATCAA TTGGGTATGA ATTTTTTTCA 
TTATTCAAGT CATGGGGAGA TACTATTACT ATATTAGTTT TGAAACCATC CAACCAGGCT 
CAGGAAATAA ATGCCTCATC ATTAGGACAA ATATACAAAC AAATGTTTGA AAAAAAGAAA 
GGTAAAATAG AAACATATTC TTACTTGGTT GATATTAAAG AAGATATCAA TTTTGAGTTT 
GTATATTTTA CAGATATCTC AAAATTGTAC AGAAGACTAT CACAGGAAAC TACTAAATTA 
AAAGAAGAAA GAGGTCTGCA GTTTTTACTC TTGTTACAAT CTCCGTTTAT CACTAAGCTC 
TTAGGCACAA TCCGGCTTCT AAACCAGATG CCCATTGTTA AGCTTTCCTT GAATGAAGTT 
CTTCTACCCC AATTGAACTG GCAACCGACA TTATTGAAGA AACTTGTTAA CCACGTTTTA 
TCCAGTGGTT CGTGGATTTC TCACTTGATC AAGTTATCCC AGTATAGTAA CATTCCAATC 
TGTAATTTGA GGCTGGATAG TATGGATTAT ATTATTGATG TTCTTTATGC AAGAAAACTA 
AAAAAAGAGA ACATCGTGCT TTGGTGGAAT GAGAAAGCTC CACTTCCAGA TCATGGAGGC 
ATTCAAAATG ATTTTGATTT AAATACATCA TGGATAATGA ATGATTCAGA ATTTCCCAAA 
ATTAATAACT CAGGTGTGTA TGACAATGTA GTTCTCGATG TTGGTGTTGA TAATTTAACA 
GTGAACACAA TTTTGACATC AGCATTAATC AATGATGCTG AAGGCAGTGA TCTAGTTAAC 
AATAATATGG GTATAGATGA CAAAGATGCC GTTATTAACT CGCCATCTGA ATTCGTGCAC 
GACGCCTTTT CTAATGACGC TTTGAATGTT TTAAGAGGTA TGTTAAAGGA GTGGTGGGAT 
GAGGCCCTAA AAGAAAATTC AACCGCAGAT TTGTTGGTAA ATTCCCTGGC AAGTTGGGTT 
CAAAACCCGA ATGCGAAACT ATTCGACGGA TTACTAAGAT ATCACGTTCA TAACTTAACA 
AAAAAAGCCT TACTTCAATT AGTAAATGAA TTTAGTGCAC TTGGCTCAAC TATTGTATAT 
GCAGACAGGA ATCAAATTCT AATAAAGACA AACAAGTACT CACCTGAAAA CTGTTACGCC 
TACAGCCAAT ATATGATGAA GGCAGTTAGA ACAAATCCAA TGTTTAGTTA TCTGGACTTA 
AATATCAAAC GTTATTGGGA TCTGCTAATA TGGATGGATA AGTTTAATTT TAGTGGATTA 
GCATGTATTG AAATAGAGGA AAAGGAAAAT CAGGATTATA CCGCTGTTTC GCAATGGCAA 
CTAAAGAAGT TTCTGTCACC AATATATCAG CCCGAATTTG AGGATTGGAT GATGATCATA 
TTGGATAGTA TGCTAAAGAC AAAGCAGAGC TATCTAAAAT TGAATTCAGG GACGCAAAGA 
CCTACCCAAA TAGTTAATGT AAAAAAACAA GATAAGGAAG ATAGTGTTGA AAACTCGTTG 
AACGGATTTT CTCACCTTTT TTCCAAACCA CTAATGAAAA GAGTCAAAAA GCTTTTTAAA 
AACCAGCAAG AGTTCATTTT AGATCCTCAG TATGAGGCAG ACTATGTTAT TCCTGTTCTT 
CCTGGTTCCC ATCTGAATGT GAAAAATCCC CTTCTAGAAC TTGTCAAATC ACTCTGCCAT 
GTCATGTTAC TTTCAAAGAG TACAATTTTA GAAATCAGGA CCCTGAGAAA AGAACTGCTG 
AAGATATTTG AATTGCGTGA GTTTGCTAAA GTAGCGGAAT TCAAAGATCC AAGTTTGAGT 
CTCGTGGTGC CGGATTTTTT ATGTGAATAC TGTTTTTTCA TTTCTGATAT TGACTTTTGT 
AAGGCAGCTC CTGAATCTAT TTTTTCATGC GTCAGATGTC ACAAAGCCTT TAATCAAGTA 
TTGTTGCAAG AACACCTGAT TCAAAAACTA CGTTCTGATA TCGAATCCTA TTTAATTCAA 
GATTTGAGAT GCTCCAGATG TCATAAAGTG AAACGTGACT ATATGAGTGC CCACTGTCCA 
TGTGCCGGCG CGTGGGAAGG AACTCTCCCC AGAGAAAGCA TTGTTCAAAA GTTAAATGTG 
TTTAAGCAAG TAGCCAAGTA TTACGGTTTT GATATATTAT TGAGTTGTAT TGCTGATTTG 
ACCATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014137.1
CDS	1..6669
Translation	MMFGKKKNNGGSSTARYSAGNKYNTLSNNYALSAQQLLNASKIDDIDSMMGFERYVPPQYNGRFDAKDIDQIPGRVGWLTNMHATLVSQETLSSGSNGGGNSNDGERVTTNQGISGVDFYFLDEEGGSFKSTVVYDPYFFIACNDESRVNDVEELVKKYLESCLKSLQIIRKEDLTMDNHLLGLQKTLIKLSFVNSNQLFEARKLLRPILQDNANNNVQRNIYNVAANGSEKVDAKHLIEDIREYDVPYHVRVSIDKDIRVGKWYKVTQQGFIEDTRKIAFADPVVMAFDIETTKPPLKFPDSAVDQIMMISYMIDGEGFLITNREIISEDIEDFEYTPKPEYPGFFTIFNENDEVALLQRFFEHIRDVRPTVISTFNGDFFDWPFIHNRSKIHGLDMFDEIGFAPDAEGEYKSSYCSHMDCFRWVKRDSYLPQGSQGLKAVTQSKLGYNPIELDPELMTPYAFEKPQHLSEYSVSDAVATYYLYMKYVHPFIFSLCTIIPLNPDETLRKGTGTLCEMLLMVQAYQHNILLPNKHTDPIERFYDGHLLESETYVGGHVESLEAGVFRSDLKNEFKIDPSAIDELLQELPEALKFSVEVENKSSVDKVTNFEEIKNQITQKLLELKENNIRNELPLIYHVDVASMYPNIMTTNRLQPDSIKAERDCASCDFNRPGKTCARKLKWAWRGEFFPSKMDEYNMIKRALQNETFPNKNKFSKKKVLTFDELSYADQVIHIKKRLTEYSRKVYHRVKVSEIVEREAIVCQRENPFYVDTVKSFRDRRYEFKGLAKTWKGNLSKIDPSDKHARDEAKKMIVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGITCLTGATIIQMARALVERVGRPLELDTDGIWCILPKSFPETYFFTLENGKKLYLSYPCSMLNYRVHQKFTNHQYQELKDPLNYIYETHSENTIFFEVDGPYKAMILPSSKEEGKGIKKRYAVFNEDGSLAELKGFELKRRGELQLIKNFQSDIFKVFLEGDTLEGCYSAVASVCNRWLDVLDSHGLMLEDEDLVSLICENRSMSKTLKEYEGQKSTSITTARRLGDFLGEDMVKDKGLQCKYIISSKPFNAPVTERAIPVAIFSADIPIKRSFLRRWTLDPSLEDLDIRTIIDWGYYRERLGSAIQKIITIPAALQGVSNPVPRVEHPDWLKRKIATKEDKFKQTSLTKFFSKTKNVPTMGKIKDIEDLFEPTVEEDNAKIKIARTTKKKAVSKRKRNQLTNEEDPLVLPSEIPSMDEDYVGWLNYQKIKWKIQARDRKRRDQLFGNTNSSRERSALGSMIRKQAESYANSTWEVLQYKDSGEPGVLEVFVTINGKVQNITFHIPKTIYMKFKSQTMPLQKIKNCLIEKSSASLPNNPKTSNPAGGQLFKITLPESVFLEEKENCTSIFNDENVLGVFEGTITPHQRAIMDLGASVTFRSKAMGALGKGIQQGFEMKDLSMAENERYLSGFSMDIGYLLHFPTSIGYEFFSLFKSWGDTITILVLKPSNQAQEINASSLGQIYKQMFEKKKGKIETYSYLVDIKEDINFEFVYFTDISKLYRRLSQETTKLKEERGLQFLLLLQSPFITKLLGTIRLLNQMPIVKLSLNEVLLPQLNWQPTLLKKLVNHVLSSGSWISHLIKLSQYSNIPICNLRLDSMDYIIDVLYARKLKKENIVLWWNEKAPLPDHGGIQNDFDLNTSWIMNDSEFPKINNSGVYDNVVLDVGVDNLTVNTILTSALINDAEGSDLVNNNMGIDDKDAVINSPSEFVHDAFSNDALNVLRGMLKEWWDEALKENSTADLLVNSLASWVQNPNAKLFDGLLRYHVHNLTKKALLQLVNEFSALGSTIVYADRNQILIKTNKYSPENCYAYSQYMMKAVRTNPMFSYLDLNIKRYWDLLIWMDKFNFSGLACIEIEEKENQDYTAVSQWQLKKFLSPIYQPEFEDWMMIILDSMLKTKQSYLKLNSGTQRPTQIVNVKKQDKEDSVENSLNGFSHLFSKPLMKRVKKLFKNQQEFILDPQYEADYVIPVLPGSHLNVKNPLLELVKSLCHVMLLSKSTILEIRTLRKELLKIFELREFAKVAEFKDPSLSLVVPDFLCEYCFFISDIDFCKAAPESIFSCVRCHKAFNQVLLQEHLIQKLRSDIESYLIQDLRCSRCHKVKRDYMSAHCPCAGAWEGTLPRESIVQKLNVFKQVAKYYGFDILLSCIADLTI

Target ORF information:

RefSeq Version	NM_001183100.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA polymerase epsilon catalytic subunit (POL2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183100.1

ORF Insert Sequence:

ATGATGTTTG GCAAGAAAAA AAACAACGGA GGATCTTCCA CTGCAAGATA TTCAGCTGGC 
AACAAGTACA ACACACTCTC AAATAATTAT GCGCTTAGCG CGCAACAGCT CTTAAATGCT 
AGTAAGATCG ATGACATCGA TTCGATGATG GGATTTGAAA GATACGTACC GCCGCAATAC 
AATGGCAGGT TTGATGCGAA GGATATAGAT CAGATTCCAG GCCGCGTAGG GTGGCTGACG 
AACATGCACG CAACGCTGGT CTCTCAGGAA ACCTTATCCA GTGGTAGTAA TGGCGGCGGC 
AATTCGAATG ACGGAGAACG TGTAACGACC AACCAAGGTA TTTCCGGAGT TGACTTCTAC 
TTTTTAGATG AAGAGGGTGG GAGCTTCAAG TCGACAGTTG TCTATGACCC ATACTTCTTT 
ATTGCGTGTA ACGATGAATC AAGAGTAAAT GATGTGGAGG AACTAGTGAA AAAATATCTG 
GAATCTTGTC TCAAAAGCTT ACAAATCATT AGAAAGGAAG ATCTTACCAT GGACAATCAC 
CTTTTAGGGC TGCAGAAGAC ACTTATTAAG TTATCATTTG TAAATTCCAA TCAGTTATTC 
GAGGCCAGGA AACTCCTGAG GCCAATCTTG CAGGATAATG CCAATAATAA TGTGCAAAGA 
AATATATATA ACGTTGCTGC AAATGGCTCG GAAAAAGTTG ACGCCAAACA TCTGATCGAA 
GATATCAGGG AATATGATGT GCCGTATCAT GTCCGAGTAT CTATAGACAA GGACATTAGA 
GTCGGTAAAT GGTATAAGGT AACTCAACAG GGATTCATTG AAGATACTAG GAAAATTGCA 
TTTGCCGACC CTGTGGTAAT GGCATTTGAT ATAGAAACCA CGAAGCCGCC TTTAAAATTC 
CCGGATTCCG CCGTAGATCA AATAATGATG ATTTCGTATA TGATCGATGG GGAAGGTTTT 
TTGATAACAA ATAGGGAGAT AATCTCTGAG GATATTGAAG ACTTTGAGTA TACACCGAAA 
CCGGAGTATC CTGGTTTTTT CACCATATTT AACGAAAACG ATGAAGTGGC GCTTCTACAA 
AGGTTTTTTG AACATATAAG AGATGTACGA CCCACTGTTA TATCCACCTT CAATGGTGAC 
TTTTTCGATT GGCCTTTTAT ACATAACAGA AGTAAGATTC ACGGCTTGGA CATGTTCGAT 
GAAATTGGTT TCGCTCCAGA TGCTGAAGGT GAGTACAAGT CCTCATACTG CTCTCACATG 
GATTGTTTCC GTTGGGTGAA GCGTGATTCT TATTTACCAC AAGGTTCCCA GGGTTTAAAA 
GCTGTTACTC AATCTAAGCT AGGTTATAAC CCAATTGAAC TGGATCCCGA ATTAATGACG 
CCGTATGCAT TTGAAAAGCC ACAGCACCTT TCCGAATATT CTGTTTCCGA TGCAGTCGCT 
ACGTATTACC TTTACATGAA ATATGTTCAT CCTTTTATCT TTTCCCTTTG TACTATTATT 
CCTTTGAACC CGGATGAAAC ATTGAGAAAG GGTACCGGTA CTTTGTGTGA AATGTTGTTG 
ATGGTTCAAG CTTATCAACA TAATATTCTT CTACCAAATA AGCATACAGA TCCCATTGAG 
AGGTTCTATG ATGGACATCT TCTAGAATCC GAGACTTACG TGGGTGGACA TGTGGAGTCA 
TTAGAAGCTG GTGTTTTTAG GAGTGATTTG AAGAATGAAT TCAAGATAGA TCCTTCTGCC 
ATTGATGAAT TATTACAAGA ATTACCAGAA GCTTTGAAAT TTAGTGTGGA AGTTGAAAAT 
AAGTCCAGTG TAGATAAAGT AACGAATTTT GAGGAAATAA AAAACCAGAT AACGCAGAAA 
TTATTAGAGT TGAAGGAAAA CAATATAAGA AACGAACTAC CTTTGATCTA TCATGTAGAT 
GTCGCCTCTA TGTACCCAAA CATCATGACT ACAAATAGAC TACAACCAGA TAGTATCAAA 
GCAGAGCGCG ATTGTGCTAG TTGCGATTTT AATAGACCCG GAAAAACCTG TGCAAGAAAG 
TTAAAATGGG CTTGGAGAGG AGAATTCTTT CCCAGTAAGA TGGATGAGTA TAACATGATC 
AAGCGTGCAT TACAAAATGA GACTTTTCCC AACAAAAACA AGTTTTCTAA AAAGAAAGTT 
TTGACATTTG ATGAACTAAG TTACGCAGAC CAAGTTATCC ACATAAAAAA ACGTTTAACT 
GAATATTCAA GGAAAGTTTA TCATAGGGTT AAAGTATCAG AAATTGTCGA ACGAGAAGCC 
ATTGTCTGCC AAAGAGAAAA TCCATTCTAC GTCGATACCG TGAAATCCTT TCGTGATAGG 
CGTTACGAAT TCAAAGGTTT AGCCAAGACT TGGAAGGGAA ATCTGTCCAA AATTGACCCA 
TCTGATAAGC ATGCGAGAGA CGAGGCCAAA AAGATGATTG TGCTTTATGA CTCATTACAA 
TTAGCTCACA AAGTTATTTT GAATTCGTTT TATGGGTATG TTATGAGGAA AGGCTCTCGT 
TGGTATTCCA TGGAAATGGC GGGGATTACG TGTTTAACAG GTGCCACGAT CATTCAAATG 
GCGAGAGCTT TAGTAGAAAG GGTAGGAAGA CCATTAGAAT TAGATACTGA TGGTATTTGG 
TGTATCTTAC CAAAATCTTT CCCTGAAACT TACTTTTTTA CATTAGAAAA TGGTAAAAAG 
CTTTATCTCT CCTACCCATG TTCCATGCTG AATTACAGAG TTCACCAAAA GTTTACGAAT 
CACCAATACC AAGAATTAAA AGACCCATTG AACTATATAT ATGAGACGCA CAGTGAAAAC 
ACGATTTTTT TCGAAGTTGA CGGACCATAT AAGGCCATGA TTTTGCCTAG TTCCAAGGAA 
GAAGGAAAAG GTATAAAGAA AAGATATGCT GTCTTCAATG AAGACGGCTC ACTTGCTGAA 
CTGAAAGGTT TTGAATTGAA GAGGCGTGGT GAATTACAAC TAATAAAAAA TTTTCAAAGT 
GATATTTTCA AGGTCTTTTT GGAAGGTGAT ACATTAGAAG GATGTTACAG TGCTGTAGCA 
AGCGTATGTA ACCGTTGGTT AGATGTTCTT GATTCACATG GTCTTATGTT AGAAGATGAA 
GACTTGGTCA GTTTGATTTG TGAAAATAGA AGTATGTCAA AAACTTTAAA GGAATATGAA 
GGGCAAAAAT CTACTTCTAT TACGACGGCA AGGAGATTGG GGGATTTTTT GGGTGAAGAT 
ATGGTAAAAG ATAAAGGTCT ACAATGTAAA TATATTATTA GTTCAAAACC TTTCAATGCA 
CCTGTTACTG AACGAGCCAT TCCAGTCGCA ATATTTTCAG CGGACATTCC CATCAAAAGG 
TCTTTTCTGA GGCGATGGAC ATTAGATCCA TCTTTGGAAG ATCTGGATAT CAGAACCATA 
ATCGATTGGG GTTATTATAG AGAAAGACTT GGATCTGCTA TACAAAAGAT AATTACTATT 
CCAGCAGCAT TACAAGGGGT TTCCAATCCT GTTCCAAGGG TTGAACATCC AGATTGGCTA 
AAAAGAAAAA TCGCTACAAA GGAGGATAAG TTTAAGCAGA CTTCACTAAC CAAATTTTTT 
TCGAAGACAA AGAATGTACC AACAATGGGC AAGATAAAAG ATATCGAGGA TTTGTTTGAA 
CCAACTGTAG AAGAAGATAA CGCCAAAATT AAAATTGCAA GAACTACTAA AAAGAAAGCC 
GTATCCAAGA GGAAAAGAAA TCAGCTTACA AATGAAGAAG ATCCACTAGT ATTGCCCTCG 
GAGATTCCTT CCATGGACGA GGACTATGTT GGGTGGCTAA ATTATCAAAA AATTAAATGG 
AAAATCCAAG CAAGAGATAG AAAGCGTCGA GACCAATTAT TTGGTAATAC AAACAGCTCC 
CGTGAAAGAA GTGCACTAGG AAGTATGATT AGGAAGCAAG CTGAATCATA TGCGAACTCC 
ACTTGGGAGG TCTTACAATA CAAGGATTCC GGTGAGCCAG GGGTTTTGGA AGTATTTGTA 
ACAATTAATG GCAAAGTCCA GAACATCACC TTCCATATAC CAAAAACTAT TTATATGAAA 
TTCAAATCTC AAACAATGCC GCTACAAAAG ATTAAGAATT GCCTTATTGA AAAATCTTCT 
GCATCGTTAC CAAATAATCC CAAAACGTCT AATCCAGCAG GCGGTCAGCT ATTCAAAATT 
ACTCTACCGG AATCTGTCTT TCTGGAAGAA AAGGAAAACT GCACTAGTAT CTTCAACGAT 
GAAAATGTAC TTGGTGTATT TGAGGGCACT ATCACTCCTC ATCAAAGAGC GATCATGGAT 
TTGGGAGCTT CGGTAACGTT CCGCTCAAAA GCAATGGGTG CGTTAGGCAA GGGAATACAG 
CAGGGTTTTG AAATGAAGGA TCTTTCAATG GCGGAAAATG AAAGGTATCT GAGTGGATTT 
TCAATGGACA TTGGCTATTT ACTACATTTC CCAACATCAA TTGGGTATGA ATTTTTTTCA 
TTATTCAAGT CATGGGGAGA TACTATTACT ATATTAGTTT TGAAACCATC CAACCAGGCT 
CAGGAAATAA ATGCCTCATC ATTAGGACAA ATATACAAAC AAATGTTTGA AAAAAAGAAA 
GGTAAAATAG AAACATATTC TTACTTGGTT GATATTAAAG AAGATATCAA TTTTGAGTTT 
GTATATTTTA CAGATATCTC AAAATTGTAC AGAAGACTAT CACAGGAAAC TACTAAATTA 
AAAGAAGAAA GAGGTCTGCA GTTTTTACTC TTGTTACAAT CTCCGTTTAT CACTAAGCTC 
TTAGGCACAA TCCGGCTTCT AAACCAGATG CCCATTGTTA AGCTTTCCTT GAATGAAGTT 
CTTCTACCCC AATTGAACTG GCAACCGACA TTATTGAAGA AACTTGTTAA CCACGTTTTA 
TCCAGTGGTT CGTGGATTTC TCACTTGATC AAGTTATCCC AGTATAGTAA CATTCCAATC 
TGTAATTTGA GGCTGGATAG TATGGATTAT ATTATTGATG TTCTTTATGC AAGAAAACTA 
AAAAAAGAGA ACATCGTGCT TTGGTGGAAT GAGAAAGCTC CACTTCCAGA TCATGGAGGC 
ATTCAAAATG ATTTTGATTT AAATACATCA TGGATAATGA ATGATTCAGA ATTTCCCAAA 
ATTAATAACT CAGGTGTGTA TGACAATGTA GTTCTCGATG TTGGTGTTGA TAATTTAACA 
GTGAACACAA TTTTGACATC AGCATTAATC AATGATGCTG AAGGCAGTGA TCTAGTTAAC 
AATAATATGG GTATAGATGA CAAAGATGCC GTTATTAACT CGCCATCTGA ATTCGTGCAC 
GACGCCTTTT CTAATGACGC TTTGAATGTT TTAAGAGGTA TGTTAAAGGA GTGGTGGGAT 
GAGGCCCTAA AAGAAAATTC AACCGCAGAT TTGTTGGTAA ATTCCCTGGC AAGTTGGGTT 
CAAAACCCGA ATGCGAAACT ATTCGACGGA TTACTAAGAT ATCACGTTCA TAACTTAACA 
AAAAAAGCCT TACTTCAATT AGTAAATGAA TTTAGTGCAC TTGGCTCAAC TATTGTATAT 
GCAGACAGGA ATCAAATTCT AATAAAGACA AACAAGTACT CACCTGAAAA CTGTTACGCC 
TACAGCCAAT ATATGATGAA GGCAGTTAGA ACAAATCCAA TGTTTAGTTA TCTGGACTTA 
AATATCAAAC GTTATTGGGA TCTGCTAATA TGGATGGATA AGTTTAATTT TAGTGGATTA 
GCATGTATTG AAATAGAGGA AAAGGAAAAT CAGGATTATA CCGCTGTTTC GCAATGGCAA 
CTAAAGAAGT TTCTGTCACC AATATATCAG CCCGAATTTG AGGATTGGAT GATGATCATA 
TTGGATAGTA TGCTAAAGAC AAAGCAGAGC TATCTAAAAT TGAATTCAGG GACGCAAAGA 
CCTACCCAAA TAGTTAATGT AAAAAAACAA GATAAGGAAG ATAGTGTTGA AAACTCGTTG 
AACGGATTTT CTCACCTTTT TTCCAAACCA CTAATGAAAA GAGTCAAAAA GCTTTTTAAA 
AACCAGCAAG AGTTCATTTT AGATCCTCAG TATGAGGCAG ACTATGTTAT TCCTGTTCTT 
CCTGGTTCCC ATCTGAATGT GAAAAATCCC CTTCTAGAAC TTGTCAAATC ACTCTGCCAT 
GTCATGTTAC TTTCAAAGAG TACAATTTTA GAAATCAGGA CCCTGAGAAA AGAACTGCTG 
AAGATATTTG AATTGCGTGA GTTTGCTAAA GTAGCGGAAT TCAAAGATCC AAGTTTGAGT 
CTCGTGGTGC CGGATTTTTT ATGTGAATAC TGTTTTTTCA TTTCTGATAT TGACTTTTGT 
AAGGCAGCTC CTGAATCTAT TTTTTCATGC GTCAGATGTC ACAAAGCCTT TAATCAAGTA 
TTGTTGCAAG AACACCTGAT TCAAAAACTA CGTTCTGATA TCGAATCCTA TTTAATTCAA 
GATTTGAGAT GCTCCAGATG TCATAAAGTG AAACGTGACT ATATGAGTGC CCACTGTCCA 
TGTGCCGGCG CGTGGGAAGG AACTCTCCCC AGAGAAAGCA TTGTTCAAAA GTTAAATGTG 
TTTAAGCAAG TAGCCAAGTA TTACGGTTTT GATATATTAT TGAGTTGTAT TGCTGATTTG 
ACCATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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POL2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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