VPS13 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS13
Entrez Gene ID	850619
Full Name	membrane morphogenesis protein VPS13
Synonyms	SOI1,VPT2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181860.1	NP_013060.1	membrane morphogenesis protein VPS13

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS13A	NP_150648.2
	LOC705323	XP_002808150.1
Canis lupus familiaris (dog)	VPS13A	XP_533522.3
Bos taurus (cattle)	VPS13A	XP_002689716.3
Drosophila melanogaster (fruit fly)	Vps13	NP_001260781.1
Saccharomyces cerevisiae (baker's yeast)	VPS13	NP_013060.1
Eremothecium gossypii	AGOS_ACL064C	NP_983340.2
Magnaporthe oryzae (rice blast fungus)	MGG_06537	XP_003717004.1
Rattus norvegicus (Norway rat)	Vps13a	NP_001094445.2
Kluyveromyces lactis	KLLA0E00705g	XP_453978.1
Pan troglodytes (chimpanzee)	VPS13A	XP_001148547.1
Mus musculus (house mouse)	Vps13a	NP_766616.2
Xenopus tropicalis (tropical clawed frog)	vps13a	XP_002937242.2
Gallus gallus (chicken)	VPS13A	NP_001264765.1
Neurospora crassa	NCU05837	XP_960097.1
Danio rerio (zebrafish)	vps13a	NP_001112365.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005082	XP_558472.3
Caenorhabditis elegans (roundworm)	T08G11.1	NP_740900.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings support a model in which Mcp1 and Vps13 work as functional effectors of vacuole-mitochondria contact sites, while tethering is mediated by other factors, including Vps39.
Title: Vps13-Mcp1 interact at vacuole-mitochondria interfaces and bypass ER-mitochondria contact sites.

These results suggest that PI3P regulates the functioning of Vps13, both in protein trafficking and actin cytoskeleton organization. Attenuation of PI3P-binding ability in the mutant hVps13A protein may be one of the reasons for its mislocalization and disrupted function in cells of patients suffering from chorea-acanthocytosis .
Title: Amino acid substitution equivalent to human chorea-acanthocytosis I2771R in yeast Vps13 protein affects its binding to phosphatidylinositol 3-phosphate.

The Vps13p-Cdc31p complex is directly required for transgolgi network late endosome transport and TGN homotypic fusion.
Title: The Vps13p-Cdc31p complex is directly required for TGN late endosome transport and TGN homotypic fusion.

his study demonstrates that VPS13 is also important for mitochondrial integrity. Introduction of VPS13A mutations identified in chorea acanthocytosis patients at cognate sites in yeast VPS13 are specifically defective in compensating for the lack of ERMES, suggesting that mitochondrial dysfunction might be the basis for chorea acanthocytosis.
Title: Yeast Vps13 promotes mitochondrial function and is localized at membrane contact sites.

Vps13 dynamically localizes to vacuole-mitochondria and to vacuole-nucleus contact sites depending on growth conditions, suggesting that ERMES function can be bypassed by the activity of other contact sites.
Title: ER-mitochondrial junctions can be bypassed by dominant mutations in the endosomal protein Vps13.

The following VPS13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00294	NM_001181860.1 Latest version!	Saccharomyces cerevisiae S288C membrane morphogenesis protein VPS13 (VPS13), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS13 ( NM_001181860.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013060.1 Saccharomyces cerevisiae S288C membrane morphogenesis protein VPS13 (VPS13), partial mRNA.

Price & Availability↑

CloneID	OSi00294
Clone ID Related Accession (Same CDS sequence)	NM_001181860.1
Accession Version	NM_001181860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	membrane morphogenesis protein VPS13
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTTAGAGT CTTTAGCTGC TAATTTGCTA AACCGATTGC TAGGCTCATA TGTGGAAAAT 
TTCGACCCTA ATCAATTAAA TGTCGGCATA TGGAGTGGTG ATGTGAAGTT AAAAAACTTA 
AAACTAAGAA AAGACTGTTT GGACTCATTA AATTTACCCA TAGATGTGAA ATCAGGGATA 
CTGGGTGATT TGGTCTTAAC GGTCCCATGG TCAAGTTTAA AGAATAAGCC CGTGAAAATC 
ATTATCGAGG ACTGTTATTT ATTATGCTCA CCACGTTCAG AAGATCACGA AAATGATGAG 
GAGATGATAA AGAGAGCTTT TCGGTTGAAG ATGAGGAAGG TAAGTGAATG GGAACTGACA 
AATCAGGCTC GCATACTGAG TACCCAATCA GAGAACAAAA CCTCTTCTTC TTCTTCAGAA 
AAAAATAACG CCGGGTTTAT GCAGTCTTTG ACTACAAAGA TCATAGACAA TTTACAAGTT 
ACCATAAAAA ATATCCATTT AAGGTACGAG GACATGGACG GAATCTTCAC TACAGGCCCA 
TCTTCCGTTG GATTAACTTT GAACGAGCTA TCAGCTGTAT CTACAGATTC CAACTGGGCT 
CCAAGTTTCA TTGATATTAC ACAGAATATC ACGCATAAAC TATTGACTTT AAATTCACTC 
TGTTTGTATT GGAATACAGA CTCTCCGCCC TTGATTTCTG ATGATGATCA AGATAGAAGC 
CTGGAAAATT TTGTAAGGGG TTTCAAAGAT ATGATTGCAT CGAAAAATTC CACTGCCCCC 
AAGCATCAAT ACATTTTGAA ACCGGTTAGC GGGCTAGGGA AGCTTAGTAT CAACAAGCTG 
GGTTCCACGG AGGAACAACC TCACATTGAC TTGCAAATGT TTTATGATGA ATTTGGTTTA 
GAATTAGATG ATACAGAATA TAATGATATC TTGCATGTCC TCTCCAGTAT ACAGTTAAGA 
CAAATTACCA AAAAGTTTAA GAAAGCAAGA CCATCCTTCG CAGTTAGTGA AAACCCGACA 
GAATGGTTTA AATACATTGC TGCTTGTGTT ATTAATGAAA TTCATGAAAA AAACAAAATG 
TGGACTTGGG AAAGCATGAA GGAGAAATGT GAACAACGGA GGTTGTACAC AAAATTATGG 
GTTGAAAAGT TAAAGTTAAA AAATTTAGAA GCTCCGCTAC GTGACCCCAT TCAGGAGGCT 
CAATTATCTG AATTGCATAA AGATTTAACG TATGATGAAA TTATTCTCTT CAGATCTGTC 
GCTAAGAGAC AGTACGCGCA ATATAAGTTG GGCATGACGG AAGACTCACC CACACCAACA 
GCTAGCTCAA ATATTGAACC ACAAACTTCC AACAAGTCCG CAACTAAAAA TAATGGAAGC 
TGGCTCTCTT CTTGGTGGAA CGGTAAACCT ACAGAAGAAG TAGACGAAGA TTTAATAATG 
ACTGAAGAAC AGCGTCAGGA GCTTTATGAT GCTATTGAAT TTGACGAGAA TGAAGATAAA 
GGTCCTGTAC TGCAAGTTCC TAGAGAAAGG GTTGAACTTC GTGTAACGAG TTTGTTAAAA 
AAGGGTTCAT TCACTATACG AAAGAAGAAG CAAAATCTAA ATTTGGGTTC CATAATTTTT 
GAAAATTGTA AAGTTGATTT TGCTCAAAGA CCTGATTCAT TTTTATCGAG TTTCCAATTG 
AACAAATTCA GTCTCGAAGA TGGCTCACCC AACGCGTTAT ATAAACATAT CATTAGTGTC 
AGAAACTCAT CTAAAGATCA ATCTTCAATT GACAACCACG CTACCGGGGA GGAAGAAGAA 
GAGGATGAAC CTTTATTACG TGCTTCATTT GAATTGAATC CACTAGATGG ATTAGCAGAT 
TCGAACTTAA ACATTAAGTT ATTGGGAATG ACTGTGTTTT ACCACGTTCA TTTCATTACA 
GAGGTCCACA AGTTTTTTAA GGCGTCAAAC CAACACATGG AAACAATCGG CAATATCGTA 
AACGCCGCCG AGGCAACTGT AGAAGGTTGG ACAACACAAA CAAGAATGGG TATTGAGTCT 
TTGTTGGAAG ACCACAAGAC CGTAAATGTA TCTTTAGATT TACAAGCACC ATTAATCATA 
TTACCGTTAG ACCCACATGA TTGGGATACC CCTTGTGCGA TCATTGATGC CGGTCATATG 
TCTATACTCA GTGATTTAGT GCCAAAGGAG AAGATAAAGG AAATAAAAGA GTTATCGCCG 
GAAGAATATG ACAAGATTGA CGGTAACGAA ATCAATAGGT TAATGTTTGA TAGGTTCCAA 
ATTTTATCTC AGGATACTCA GATATTTGTT GGTCCTGATA TTCAGTCTAC TATAGGTAAG 
ATCAATACCG CCTCTTCCAC GAATGATTTT AGAATCTTGG ATAAAATGAA ATTAGAGTTA 
ACTGTAGATC TATCAATCCT TCCTAAAGCA TATAAATTAC CCACGATAAG AGTTTTTGGT 
CATTTGCCTC GCTTAAGTCT TTCCATAAAT GACATTCAAT ACAAGACTAT TATGAATTTG 
ATTGCTAATT CTATACCTTC TATGATTGAT GATGAAGAAA ACAACGGCGA TTACGTTAAT 
TACTCTTCTG GTTCCGAAAA AGAAATGAAG AAGCAAATTC AATTGCAGTT GAAGAATACT 
TTGAAAGCGT TAGAAAATAT GCAGCCGCTT CAAATTGAAC AGAAATTCTT AGAGCTCCAT 
TTTGATATCG ATCAGGCGAA AATAGCATTC TTTCAATGTA TCAAAAATGA TTCCAGAAAC 
AGCGAAAAAT TGGTTGATAT ATTGTGTCAA AGGCTAAATT TCAATTTTGA TAAGAGAGCC 
AAAGAAATGA ATTTAGATCT AAGAGTACAT TCATTAGATG TTGAAGATTA CATCGAGCTG 
ACTGATAACA AGGAATTCAA AAACTTGATA TCATCCGGCG TTGAAAAAGT GACAAGATCT 
CAAAAAGATT TGTTTACGCT AAAATACAAG AGAGTGCAGA GAATTGTGCC TCATAACGAT 
ACTTTGATTG AATTATTCGA TCAAGATATT GTTATGCATA TGAGTGAATT GCAATTGGTA 
TTAACACCTA GATCTGTATT AACTTTAATG AATTATGCAA TGCTTACTTT TACGGATCCT 
AACGCTCCAG AAATGCCGGC TGATGTGTTG AGACATAATA AAGAAGATAG GGACGATGCG 
CCACAAAAAA TCAATATGAA AATTAAAATG GAGGCCGTCA ATGTTATTTT TAATGATGAT 
TCTATAAAGC TGGCAACGTT AGTTCTATCG GCTGGTGAAT TCACAATGGT TTTACTACCG 
GAAAGATACA ACATTAACTT AAAATTAGGC GGGTTGGAGT TGACTGATGA AACAAATGAA 
TCGTTTTCCA GGGATTCGGT ATTTAGAAAA ATAATTCAAA TGAAAGGTCA AGAACTAGTC 
GAATTGAGCT ATGAATCATT CGATCCGGCT ACAAATACCA AAGATTATGA TTCGTTTTTA 
AAATATTCTA CGGGCTCTAT GCATGTTAAT TTTATCGAAA GTGCTGTTAA CAGAATGGTT 
AACTTTTTTG CTAAATTTCA AAAATCTAAA GTATCCTTTG ATAGAGCTCG TCTTGCAGCG 
TACAACCAAG CCCCTTCCAT TGACGCCGTA AATAACATGA AAATGGACAT AGTAATCAAG 
GCACCTATTA TCCAGTTTCC TAAATTAGTA GGCACACAAG AGAACAATTA CGATACAATG 
CGGTTTTATT TGGGTGAGTT CTTTATTGAA AACAAATTTT CGGTAATAGA CGAATCTCAT 
AAAATAAACC ATATCAAACT TGGTGTCCGC GAGGGCCAAT TATCCTCGAA CTTAAACTTC 
GATGGTTCCA GTCAACAATT GTATCTAGTT GAAAATATAG GTTTACTTTT CAATATAGAC 
AGAGATCCCC TTCCGCAAGA TGACACTCCA GAACTTAAAG TAACGAGTAA TTTTGAATCA 
TTTGCGTTGG ATTTGACTGA GAACCAGCTG ACTTACTTAT TAGAAATTTC CAATAAAGTA 
TCCTCTGCAT TCAATATCAC AGACGAAAAC TCTGGTGAAT CAGGAGGAAA AGGCGAGATC 
AAAAGTCCCT CTCCTGACCC TGCTTCTTTA AGTAGCGAAA GCGAACGCAC AGCTACTCCT 
CAGTCTCTAC AGGGATCGAA TAAAAGCAAT ATAAAAAATC CAGAACAAAA ATACTTGGAT 
TTTTCTTTTA AAGCTCCAAA GATTGCCCTT ACTTTATATA ACAAGACAAA GGGCGTCACT 
TCTTTGAATG ACTGTGGTCT GACAAGAATT ATGTTTCAAG ACATTGGGTG CAGCCTTGGT 
TTAAAGAATG ATGGGACCGT CGATGGTCAA GCACATGTTG CTGCTTTCCG GATAGAAGAT 
GTTCGTAATA TAAAAGATAA TAAGCATACA GAACTAATAC CAAAGAGTAA GAATAAAGAA 
TATCAATTTG TGGCTAATAT TTCCAGAAAG AACTTAGAGG TCGGACGGCT ATTGAACATT 
TCGATGACAA TGGACAGTCC GAAAATGATT TTGGCAATGG ACTATTTGGT TTCTCTCAAA 
GAATTTTTTG ATGCAATTAT GTCCAAAAGC CACGAAAACA ATTTATACTA TCCTGAAAAC 
ACAAACCAAA AGCCTGAAAA CAAGGCTATT GTTGAAAGCG TTCAAGAAGG TGGTGATGTG 
ACTAAAATCC AATACTCTGT TAATATCATC GAAACGGCTC TGATCTTACT TGCTGATCCT 
TGCGACATGA ATAGTGAGGC TATTTCTTTC AAGATAGGAC AATTTTTAGT AACCGATCAG 
AATATTATGA CAGTTGCAGC CAATAATGTT GGTATCTTCC TATTCAAAAT GAATTCGTCT 
GAAGAAAAAT TGAGATTATT GGACGACTTT TCATCCTCCT TGACAATTGA TAAAAGAAAC 
TCAACTCCTC AGACCCTAAT GACGAATATA CAGCTTTCCG TTCAGCCCCT GTTGATGCGT 
ATTTCACTTA GAGATATTCG TCTGGCGATG TTAATATTCA AACGAGTTAC CACATTGCTA 
AATAAGATGA CGGAAAAAGA GGATAATGGA GAGGAGGAGG AGAGCACAGA CAAAATACAA 
TTTTCTCATG AATTTGAAAG GAAATTAGCG GTGTTGGATC CAAGCATTTT AGGTGAGCGC 
AGCAGGGCTA GCCAGAGCTC AGATTCCGAA TCAATTGAAG TGCCTACTGC AATTTTGAAA 
AACGAAACAT TCAATGCTGA CTTAGGCGGA CTGAGATTTA TTTTAATTGG TGACGTCCAT 
GAAATGCCAA TATTGGACAT GAATGTAAAT GAAATCACTG CTTCAGCAAA GGATTGGTCT 
ACCGACTTTG AAGCTCTTGC TTCTCTAGAA ACCTATGTTA ACATATTCAA CTATTCGAGA 
TCTAGCTGGG AGCCACTTCT GGAGATGATT CCAATAACAT TCCACCTATC AAAAGGGCAT 
TCTGAAATGG ATCCTGCTTT CTCTTTTGAT ATTTTAACTC AAAGAATCGC GGAAATTACA 
CTTTCTGCTA GGTCAATCGC TATGCTTTCT CACATCCCGG CGTCTCTAAC CGAAGAGCTG 
CCCTTAGCTT CCAGGGTATC TCAGAAGCCA TATCAACTGG TAAACGATAC AGAATTAGAT 
TTTGACGTCT GGATACAGGA TAAAACGACT GAAGACAACA AAAACGAAGT TGTGCTCTTA 
AAAGCAAACA CTTCTCTTCC TTGGGAATTT GAAGATTGGA GAAGTATTAG AGAGAAATTG 
GATATTGATA AGTCGAAGAA CATTTTGGGT GTTTGTGTTT CTGGGCAAAA TTATAAAACC 
ATTATGAATA TTGATGCCAC AACAGAAGGT GAAAACTTGC ATGTTTTGAG CCCACCAAGA 
AATAATGTGC ATAATAGAAT TGTTTGTGAG GCCAGATGTG ATGAGAATAA CGTGAAGATT 
ATTACGTTTA GGTCAACTTT AGTTATCGAA AACACAACTT CAACTGAGAT TGAGCTTTTG 
GTTGACTCCA AAGATCCAAA TAAGCCATCT TTAAAATATG CTATTAAACC ACATCAAAGT 
AAATCTGTTC CTGTAGAATA TGCCTACGAT TCTGATATTA GGATAAGACC CGCATCAGAG 
GATATATACG ATTGGTCTCA ACAGACGTTA TCTTGGAAAA GCTTGTTGAG CAACCAAATG 
TCTATATTCT GTTCTTCAAA AGAAGATTCC AATCAAAGAT TTCATTTCGA AATTGGTGCA 
AAGTATGATG AAAGAGAACC ATTGGCAAAG ATCTTTCCTC ATATGAAAAT CGTGGTTTCG 
GCATCTATGA CTATTGAAAA TTTACTTCCA GCAGATATTA ACTTCTCTAT TTTTGACAAA 
AGAGAGGAGA AGAGAACAGA TTTCTTGAAG ACTGGAGAAA GTATGGAAGT TCATCATATT 
TCTTTGGATA GTTTCCTGCT AATGTCTGTT CAACCATTAC AAGATGAGGC TTCTGCTTCA 
AAGCCCTCCA TCGTTAATAC ACCACATAAA AGTCCTTTAA ACCCAGAAGA TTCGTTGTCT 
TTGACCCTAT CTGGAGGTCA GAATTTGTTG TTGAAACTAG ATTATAAAAA CATCGATGGT 
ACAAGGTCCA AAGTTATTCG GATATACTCC CCTTATATTA TTATGAACTC TACCGATAGA 
GAACTCTACA TTCAAAGTAG TCTCTTAAAT ATTGCACAAT CCAAGATTTT GCTTGAAAAC 
GAAAAAAGAT ATACTATTCC AAAAATGTTT TCATTTGATA AAGAAGATGA TAAAAGTAAC 
AGAGCTCGTA TTAGGTTCAA GGAATCAGAG TGGAGTTCCA AATTATCATT TGACGCTATT 
GGCCAATCAT TTGATGCATC TGTTCGCATA AAAAATAAGG AACAAGAATC AAATTTAGGC 
ATCAACATTA GCGAAGGTAA AGGGAAGTAC TTGCTAAGTA AAGTTATTGA GATTGCACCT 
AGGTATATTA TAAGCAATAC TTTAGATATC CCTATTGAAG TTTGCGAGAC AGGGTCTATG 
GATGTCCAGC AAATAGAATC GAACATCACT AAGCCTTTAT ACAGGATGAG AAACATCGTC 
GACAAGCAAT TAGTGCTGAA ATTCTTAGGC GGAGACTCCA ATTGGTCACA ACCATTCTTT 
ATCAAAAATG TCGGTGTAAC ATATTTGAAA GTTTTGAAAA ATTCACGCCA TAAGCTTTTA 
AAGATTGAAA TTCTTTTGGA CAAAGCAACA ATATTCATAA GAATCAAGGA CGGTGGTGAC 
AGATGGCCAT TTTCCATAAG AAATTTCAGC GACCATGACT TTATATTTTA CCAAAGAGAT 
CCTCGTAAAG TTAGTGATCC ATACAAGGAT GACCAGTCTA ACGAGTCCTC AAGCCGTAGT 
TTCAAACCTA TCTTTTACAG GATTCCCTCG AAAAGTATTA TGCCATACGC ATGGGATTTT 
CCTACAGCTA AGGAGAAATA TCTGGTGTTA GAATCAGGTA CACGTACAAG GGAAGTTCGG 
TTGGCTGAGA TTGGCGAATT GCCACCGTTA CGCCTGGATA AAAGGTCAAA GGATAAACCA 
GCCCCCATTG TTGGATTACA TGTTGTTGCT GACGACGATA TGCAGGCCTT AGTTATTGTT 
AACTATAAGG CCAATGTTGG GTTATACAAA TTGAAAACTG CCTCTGCAAC TACCACGTCT 
TCAGTTTCAG TAAATAGTTC AGTGACTGAT GGCTTTGTGC AAAAGGATGA GGATGAGAAG 
GTTAATACAC AGATAGTTGT TTCGTTCAAA GGAGTTGGTA TCTCATTAAT CAATGGTCGT 
TTACAGGAAC TTCTATATAT TAACATGAGA GGGATCGAAT TAAGATACAA TGAATCTAAA 
GCATACCAGA CTTTCAGTTG GAAAATGAAA TGGATGCAAA TAGATAATCA ATTATTTTCA 
GGAAACTACT CAAATATTCT ATATCCAACA GAAATCCCCT ACACAGAAAA GGAAATTGAA 
AATCATCCAG TGATATCAGG TTCTATTTCT AAAGTCAACG ATTCCTTACA GGCTGTCCCA 
TACTTTAAAC ATGTTACGTT ATTAATCCAG GAATTTTCCA TACAATTAGA TGAAGACATG 
CTGTATGCTA TGATGGACTT CATAAAATTC CCAGGATCCC CATGGATCAT GGATTCAAGA 
GATTATAAGT ACGATGAAGA AATCCAGTTA CCTGATGTTT CTGAATTAAA AACGGCGGGT 
GATATATATT TCGAGATCTT CCATATTCAG CCCACCGTCT TACATCTGTC CTTTATACGC 
TCCGATGAAA TTAGTCCAGG ATTGGCTGAA GAGACCGAGG AGTCATTTTC GTCATCATTG 
TACTATGTCC ATATGTTTGC TATGACATTA GGTAATATAA ATGAGGCGCC GGTTAAAGTT 
AATTCTTTGT TCATGGATAA TGTAAGAGTC CCATTGCCCA TTCTGATGGA CCATATTGAG 
AGACACTATA CGACCCAGTT CGTGTATCAG ATCCACAAAA TTTTAGGATC AGCAGATTGT 
TTTGGTAATC CTGTAGGTCT TTTCAACACA ATTAGTTCCG GTGTCTGGGA CTTATTCTAT 
GAACCATATC AAGGTTACAT GATGAATGAT AGGCCTCAAG AAATTGGTAT ACATCTGGCT 
AAAGGTGGAC TAAGTTTTGC AAAGAAAACA GTCTTTGGCC TCTCTGATAG TATGTCCAAG 
TTTACGGGGT CGATGGCCAA GGGCCTGTCA GTTACACAAG ATCTGGAATT CCAAAGGGTC 
AGGAGATTAC AACAAAGAAT AAATAAAAAC AACAGGAATG CCTTGGCAAA CTCAGCGCAA 
TCCTTTGCCA GCACACTGGG AAGCGGGTTG AGCGGAATTG CACTGGACCC ATACAAGGCC 
ATGCAAAAGG AAGGCGCCGC TGGTTTCCTC AAAGGCCTTG GTAAAGGTAT CGTTGGGCTG 
CCAACTAAGA CAGCCATTGG ATTCTTAGAT CTTACAAGTA ACTTAAGTCA AGGCGTGAAA 
AGTACCACCA CTGTGCTTGA CATGCAAAAG GGTTGCAGAG TGCGTCTACC AAGGTACGTG 
GACCATGATC AAATCATCAA GCCTTATGAT CTACGTGAGG CCCAAGGACA ATACTGGCTC 
AAGACCGTGA ATGGAGGAGT ATTTATGAAT GATGAATACC TGTCGCACGT TATATTGCCA 
GGCAAAGAAC TAGCAGTAAT TGTGTCCATG CAGCACATTG CTGAGGTGCA AATGGCCACC 
CAAGAGCTTA TGTGGAGCAC AGGATATCCT TCCATCCAGG GAATTACATT GGAAAGATCC 
GGTCTTCAAA TTAAACTCAA GTCTCAATCC GAATACTTCA TTCCCATCAG CGATCCTGAA 
GAACGTCGGT CCTTGTACAG GAACATCGCC ATTGCTGTTA GAGAATACAA TAAGTACTGT 
GAAGCTATCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013060.1
CDS	1..9435
Translation	MLESLAANLLNRLLGSYVENFDPNQLNVGIWSGDVKLKNLKLRKDCLDSLNLPIDVKSGILGDLVLTVPWSSLKNKPVKIIIEDCYLLCSPRSEDHENDEEMIKRAFRLKMRKVSEWELTNQARILSTQSENKTSSSSSEKNNAGFMQSLTTKIIDNLQVTIKNIHLRYEDMDGIFTTGPSSVGLTLNELSAVSTDSNWAPSFIDITQNITHKLLTLNSLCLYWNTDSPPLISDDDQDRSLENFVRGFKDMIASKNSTAPKHQYILKPVSGLGKLSINKLGSTEEQPHIDLQMFYDEFGLELDDTEYNDILHVLSSIQLRQITKKFKKARPSFAVSENPTEWFKYIAACVINEIHEKNKMWTWESMKEKCEQRRLYTKLWVEKLKLKNLEAPLRDPIQEAQLSELHKDLTYDEIILFRSVAKRQYAQYKLGMTEDSPTPTASSNIEPQTSNKSATKNNGSWLSSWWNGKPTEEVDEDLIMTEEQRQELYDAIEFDENEDKGPVLQVPRERVELRVTSLLKKGSFTIRKKKQNLNLGSIIFENCKVDFAQRPDSFLSSFQLNKFSLEDGSPNALYKHIISVRNSSKDQSSIDNHATGEEEEEDEPLLRASFELNPLDGLADSNLNIKLLGMTVFYHVHFITEVHKFFKASNQHMETIGNIVNAAEATVEGWTTQTRMGIESLLEDHKTVNVSLDLQAPLIILPLDPHDWDTPCAIIDAGHMSILSDLVPKEKIKEIKELSPEEYDKIDGNEINRLMFDRFQILSQDTQIFVGPDIQSTIGKINTASSTNDFRILDKMKLELTVDLSILPKAYKLPTIRVFGHLPRLSLSINDIQYKTIMNLIANSIPSMIDDEENNGDYVNYSSGSEKEMKKQIQLQLKNTLKALENMQPLQIEQKFLELHFDIDQAKIAFFQCIKNDSRNSEKLVDILCQRLNFNFDKRAKEMNLDLRVHSLDVEDYIELTDNKEFKNLISSGVEKVTRSQKDLFTLKYKRVQRIVPHNDTLIELFDQDIVMHMSELQLVLTPRSVLTLMNYAMLTFTDPNAPEMPADVLRHNKEDRDDAPQKINMKIKMEAVNVIFNDDSIKLATLVLSAGEFTMVLLPERYNINLKLGGLELTDETNESFSRDSVFRKIIQMKGQELVELSYESFDPATNTKDYDSFLKYSTGSMHVNFIESAVNRMVNFFAKFQKSKVSFDRARLAAYNQAPSIDAVNNMKMDIVIKAPIIQFPKLVGTQENNYDTMRFYLGEFFIENKFSVIDESHKINHIKLGVREGQLSSNLNFDGSSQQLYLVENIGLLFNIDRDPLPQDDTPELKVTSNFESFALDLTENQLTYLLEISNKVSSAFNITDENSGESGGKGEIKSPSPDPASLSSESERTATPQSLQGSNKSNIKNPEQKYLDFSFKAPKIALTLYNKTKGVTSLNDCGLTRIMFQDIGCSLGLKNDGTVDGQAHVAAFRIEDVRNIKDNKHTELIPKSKNKEYQFVANISRKNLEVGRLLNISMTMDSPKMILAMDYLVSLKEFFDAIMSKSHENNLYYPENTNQKPENKAIVESVQEGGDVTKIQYSVNIIETALILLADPCDMNSEAISFKIGQFLVTDQNIMTVAANNVGIFLFKMNSSEEKLRLLDDFSSSLTIDKRNSTPQTLMTNIQLSVQPLLMRISLRDIRLAMLIFKRVTTLLNKMTEKEDNGEEEESTDKIQFSHEFERKLAVLDPSILGERSRASQSSDSESIEVPTAILKNETFNADLGGLRFILIGDVHEMPILDMNVNEITASAKDWSTDFEALASLETYVNIFNYSRSSWEPLLEMIPITFHLSKGHSEMDPAFSFDILTQRIAEITLSARSIAMLSHIPASLTEELPLASRVSQKPYQLVNDTELDFDVWIQDKTTEDNKNEVVLLKANTSLPWEFEDWRSIREKLDIDKSKNILGVCVSGQNYKTIMNIDATTEGENLHVLSPPRNNVHNRIVCEARCDENNVKIITFRSTLVIENTTSTEIELLVDSKDPNKPSLKYAIKPHQSKSVPVEYAYDSDIRIRPASEDIYDWSQQTLSWKSLLSNQMSIFCSSKEDSNQRFHFEIGAKYDEREPLAKIFPHMKIVVSASMTIENLLPADINFSIFDKREEKRTDFLKTGESMEVHHISLDSFLLMSVQPLQDEASASKPSIVNTPHKSPLNPEDSLSLTLSGGQNLLLKLDYKNIDGTRSKVIRIYSPYIIMNSTDRELYIQSSLLNIAQSKILLENEKRYTIPKMFSFDKEDDKSNRARIRFKESEWSSKLSFDAIGQSFDASVRIKNKEQESNLGINISEGKGKYLLSKVIEIAPRYIISNTLDIPIEVCETGSMDVQQIESNITKPLYRMRNIVDKQLVLKFLGGDSNWSQPFFIKNVGVTYLKVLKNSRHKLLKIEILLDKATIFIRIKDGGDRWPFSIRNFSDHDFIFYQRDPRKVSDPYKDDQSNESSSRSFKPIFYRIPSKSIMPYAWDFPTAKEKYLVLESGTRTREVRLAEIGELPPLRLDKRSKDKPAPIVGLHVVADDDMQALVIVNYKANVGLYKLKTASATTTSSVSVNSSVTDGFVQKDEDEKVNTQIVVSFKGVGISLINGRLQELLYINMRGIELRYNESKAYQTFSWKMKWMQIDNQLFSGNYSNILYPTEIPYTEKEIENHPVISGSISKVNDSLQAVPYFKHVTLLIQEFSIQLDEDMLYAMMDFIKFPGSPWIMDSRDYKYDEEIQLPDVSELKTAGDIYFEIFHIQPTVLHLSFIRSDEISPGLAEETEESFSSSLYYVHMFAMTLGNINEAPVKVNSLFMDNVRVPLPILMDHIERHYTTQFVYQIHKILGSADCFGNPVGLFNTISSGVWDLFYEPYQGYMMNDRPQEIGIHLAKGGLSFAKKTVFGLSDSMSKFTGSMAKGLSVTQDLEFQRVRRLQQRINKNNRNALANSAQSFASTLGSGLSGIALDPYKAMQKEGAAGFLKGLGKGIVGLPTKTAIGFLDLTSNLSQGVKSTTTVLDMQKGCRVRLPRYVDHDQIIKPYDLREAQGQYWLKTVNGGVFMNDEYLSHVILPGKELAVIVSMQHIAEVQMATQELMWSTGYPSIQGITLERSGLQIKLKSQSEYFIPISDPEERRSLYRNIAIAVREYNKYCEAIL

Target ORF information:

RefSeq Version	NM_001181860.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C membrane morphogenesis protein VPS13 (VPS13), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181860.1

ORF Insert Sequence:

ATGTTAGAGT CTTTAGCTGC TAATTTGCTA AACCGATTGC TAGGCTCATA TGTGGAAAAT 
TTCGACCCTA ATCAATTAAA TGTCGGCATA TGGAGTGGTG ATGTGAAGTT AAAAAACTTA 
AAACTAAGAA AAGACTGTTT GGACTCATTA AATTTACCCA TAGATGTGAA ATCAGGGATA 
CTGGGTGATT TGGTCTTAAC GGTCCCATGG TCAAGTTTAA AGAATAAGCC CGTGAAAATC 
ATTATCGAGG ACTGTTATTT ATTATGCTCA CCACGTTCAG AAGATCACGA AAATGATGAG 
GAGATGATAA AGAGAGCTTT TCGGTTGAAG ATGAGGAAGG TAAGTGAATG GGAACTGACA 
AATCAGGCTC GCATACTGAG TACCCAATCA GAGAACAAAA CCTCTTCTTC TTCTTCAGAA 
AAAAATAACG CCGGGTTTAT GCAGTCTTTG ACTACAAAGA TCATAGACAA TTTACAAGTT 
ACCATAAAAA ATATCCATTT AAGGTACGAG GACATGGACG GAATCTTCAC TACAGGCCCA 
TCTTCCGTTG GATTAACTTT GAACGAGCTA TCAGCTGTAT CTACAGATTC CAACTGGGCT 
CCAAGTTTCA TTGATATTAC ACAGAATATC ACGCATAAAC TATTGACTTT AAATTCACTC 
TGTTTGTATT GGAATACAGA CTCTCCGCCC TTGATTTCTG ATGATGATCA AGATAGAAGC 
CTGGAAAATT TTGTAAGGGG TTTCAAAGAT ATGATTGCAT CGAAAAATTC CACTGCCCCC 
AAGCATCAAT ACATTTTGAA ACCGGTTAGC GGGCTAGGGA AGCTTAGTAT CAACAAGCTG 
GGTTCCACGG AGGAACAACC TCACATTGAC TTGCAAATGT TTTATGATGA ATTTGGTTTA 
GAATTAGATG ATACAGAATA TAATGATATC TTGCATGTCC TCTCCAGTAT ACAGTTAAGA 
CAAATTACCA AAAAGTTTAA GAAAGCAAGA CCATCCTTCG CAGTTAGTGA AAACCCGACA 
GAATGGTTTA AATACATTGC TGCTTGTGTT ATTAATGAAA TTCATGAAAA AAACAAAATG 
TGGACTTGGG AAAGCATGAA GGAGAAATGT GAACAACGGA GGTTGTACAC AAAATTATGG 
GTTGAAAAGT TAAAGTTAAA AAATTTAGAA GCTCCGCTAC GTGACCCCAT TCAGGAGGCT 
CAATTATCTG AATTGCATAA AGATTTAACG TATGATGAAA TTATTCTCTT CAGATCTGTC 
GCTAAGAGAC AGTACGCGCA ATATAAGTTG GGCATGACGG AAGACTCACC CACACCAACA 
GCTAGCTCAA ATATTGAACC ACAAACTTCC AACAAGTCCG CAACTAAAAA TAATGGAAGC 
TGGCTCTCTT CTTGGTGGAA CGGTAAACCT ACAGAAGAAG TAGACGAAGA TTTAATAATG 
ACTGAAGAAC AGCGTCAGGA GCTTTATGAT GCTATTGAAT TTGACGAGAA TGAAGATAAA 
GGTCCTGTAC TGCAAGTTCC TAGAGAAAGG GTTGAACTTC GTGTAACGAG TTTGTTAAAA 
AAGGGTTCAT TCACTATACG AAAGAAGAAG CAAAATCTAA ATTTGGGTTC CATAATTTTT 
GAAAATTGTA AAGTTGATTT TGCTCAAAGA CCTGATTCAT TTTTATCGAG TTTCCAATTG 
AACAAATTCA GTCTCGAAGA TGGCTCACCC AACGCGTTAT ATAAACATAT CATTAGTGTC 
AGAAACTCAT CTAAAGATCA ATCTTCAATT GACAACCACG CTACCGGGGA GGAAGAAGAA 
GAGGATGAAC CTTTATTACG TGCTTCATTT GAATTGAATC CACTAGATGG ATTAGCAGAT 
TCGAACTTAA ACATTAAGTT ATTGGGAATG ACTGTGTTTT ACCACGTTCA TTTCATTACA 
GAGGTCCACA AGTTTTTTAA GGCGTCAAAC CAACACATGG AAACAATCGG CAATATCGTA 
AACGCCGCCG AGGCAACTGT AGAAGGTTGG ACAACACAAA CAAGAATGGG TATTGAGTCT 
TTGTTGGAAG ACCACAAGAC CGTAAATGTA TCTTTAGATT TACAAGCACC ATTAATCATA 
TTACCGTTAG ACCCACATGA TTGGGATACC CCTTGTGCGA TCATTGATGC CGGTCATATG 
TCTATACTCA GTGATTTAGT GCCAAAGGAG AAGATAAAGG AAATAAAAGA GTTATCGCCG 
GAAGAATATG ACAAGATTGA CGGTAACGAA ATCAATAGGT TAATGTTTGA TAGGTTCCAA 
ATTTTATCTC AGGATACTCA GATATTTGTT GGTCCTGATA TTCAGTCTAC TATAGGTAAG 
ATCAATACCG CCTCTTCCAC GAATGATTTT AGAATCTTGG ATAAAATGAA ATTAGAGTTA 
ACTGTAGATC TATCAATCCT TCCTAAAGCA TATAAATTAC CCACGATAAG AGTTTTTGGT 
CATTTGCCTC GCTTAAGTCT TTCCATAAAT GACATTCAAT ACAAGACTAT TATGAATTTG 
ATTGCTAATT CTATACCTTC TATGATTGAT GATGAAGAAA ACAACGGCGA TTACGTTAAT 
TACTCTTCTG GTTCCGAAAA AGAAATGAAG AAGCAAATTC AATTGCAGTT GAAGAATACT 
TTGAAAGCGT TAGAAAATAT GCAGCCGCTT CAAATTGAAC AGAAATTCTT AGAGCTCCAT 
TTTGATATCG ATCAGGCGAA AATAGCATTC TTTCAATGTA TCAAAAATGA TTCCAGAAAC 
AGCGAAAAAT TGGTTGATAT ATTGTGTCAA AGGCTAAATT TCAATTTTGA TAAGAGAGCC 
AAAGAAATGA ATTTAGATCT AAGAGTACAT TCATTAGATG TTGAAGATTA CATCGAGCTG 
ACTGATAACA AGGAATTCAA AAACTTGATA TCATCCGGCG TTGAAAAAGT GACAAGATCT 
CAAAAAGATT TGTTTACGCT AAAATACAAG AGAGTGCAGA GAATTGTGCC TCATAACGAT 
ACTTTGATTG AATTATTCGA TCAAGATATT GTTATGCATA TGAGTGAATT GCAATTGGTA 
TTAACACCTA GATCTGTATT AACTTTAATG AATTATGCAA TGCTTACTTT TACGGATCCT 
AACGCTCCAG AAATGCCGGC TGATGTGTTG AGACATAATA AAGAAGATAG GGACGATGCG 
CCACAAAAAA TCAATATGAA AATTAAAATG GAGGCCGTCA ATGTTATTTT TAATGATGAT 
TCTATAAAGC TGGCAACGTT AGTTCTATCG GCTGGTGAAT TCACAATGGT TTTACTACCG 
GAAAGATACA ACATTAACTT AAAATTAGGC GGGTTGGAGT TGACTGATGA AACAAATGAA 
TCGTTTTCCA GGGATTCGGT ATTTAGAAAA ATAATTCAAA TGAAAGGTCA AGAACTAGTC 
GAATTGAGCT ATGAATCATT CGATCCGGCT ACAAATACCA AAGATTATGA TTCGTTTTTA 
AAATATTCTA CGGGCTCTAT GCATGTTAAT TTTATCGAAA GTGCTGTTAA CAGAATGGTT 
AACTTTTTTG CTAAATTTCA AAAATCTAAA GTATCCTTTG ATAGAGCTCG TCTTGCAGCG 
TACAACCAAG CCCCTTCCAT TGACGCCGTA AATAACATGA AAATGGACAT AGTAATCAAG 
GCACCTATTA TCCAGTTTCC TAAATTAGTA GGCACACAAG AGAACAATTA CGATACAATG 
CGGTTTTATT TGGGTGAGTT CTTTATTGAA AACAAATTTT CGGTAATAGA CGAATCTCAT 
AAAATAAACC ATATCAAACT TGGTGTCCGC GAGGGCCAAT TATCCTCGAA CTTAAACTTC 
GATGGTTCCA GTCAACAATT GTATCTAGTT GAAAATATAG GTTTACTTTT CAATATAGAC 
AGAGATCCCC TTCCGCAAGA TGACACTCCA GAACTTAAAG TAACGAGTAA TTTTGAATCA 
TTTGCGTTGG ATTTGACTGA GAACCAGCTG ACTTACTTAT TAGAAATTTC CAATAAAGTA 
TCCTCTGCAT TCAATATCAC AGACGAAAAC TCTGGTGAAT CAGGAGGAAA AGGCGAGATC 
AAAAGTCCCT CTCCTGACCC TGCTTCTTTA AGTAGCGAAA GCGAACGCAC AGCTACTCCT 
CAGTCTCTAC AGGGATCGAA TAAAAGCAAT ATAAAAAATC CAGAACAAAA ATACTTGGAT 
TTTTCTTTTA AAGCTCCAAA GATTGCCCTT ACTTTATATA ACAAGACAAA GGGCGTCACT 
TCTTTGAATG ACTGTGGTCT GACAAGAATT ATGTTTCAAG ACATTGGGTG CAGCCTTGGT 
TTAAAGAATG ATGGGACCGT CGATGGTCAA GCACATGTTG CTGCTTTCCG GATAGAAGAT 
GTTCGTAATA TAAAAGATAA TAAGCATACA GAACTAATAC CAAAGAGTAA GAATAAAGAA 
TATCAATTTG TGGCTAATAT TTCCAGAAAG AACTTAGAGG TCGGACGGCT ATTGAACATT 
TCGATGACAA TGGACAGTCC GAAAATGATT TTGGCAATGG ACTATTTGGT TTCTCTCAAA 
GAATTTTTTG ATGCAATTAT GTCCAAAAGC CACGAAAACA ATTTATACTA TCCTGAAAAC 
ACAAACCAAA AGCCTGAAAA CAAGGCTATT GTTGAAAGCG TTCAAGAAGG TGGTGATGTG 
ACTAAAATCC AATACTCTGT TAATATCATC GAAACGGCTC TGATCTTACT TGCTGATCCT 
TGCGACATGA ATAGTGAGGC TATTTCTTTC AAGATAGGAC AATTTTTAGT AACCGATCAG 
AATATTATGA CAGTTGCAGC CAATAATGTT GGTATCTTCC TATTCAAAAT GAATTCGTCT 
GAAGAAAAAT TGAGATTATT GGACGACTTT TCATCCTCCT TGACAATTGA TAAAAGAAAC 
TCAACTCCTC AGACCCTAAT GACGAATATA CAGCTTTCCG TTCAGCCCCT GTTGATGCGT 
ATTTCACTTA GAGATATTCG TCTGGCGATG TTAATATTCA AACGAGTTAC CACATTGCTA 
AATAAGATGA CGGAAAAAGA GGATAATGGA GAGGAGGAGG AGAGCACAGA CAAAATACAA 
TTTTCTCATG AATTTGAAAG GAAATTAGCG GTGTTGGATC CAAGCATTTT AGGTGAGCGC 
AGCAGGGCTA GCCAGAGCTC AGATTCCGAA TCAATTGAAG TGCCTACTGC AATTTTGAAA 
AACGAAACAT TCAATGCTGA CTTAGGCGGA CTGAGATTTA TTTTAATTGG TGACGTCCAT 
GAAATGCCAA TATTGGACAT GAATGTAAAT GAAATCACTG CTTCAGCAAA GGATTGGTCT 
ACCGACTTTG AAGCTCTTGC TTCTCTAGAA ACCTATGTTA ACATATTCAA CTATTCGAGA 
TCTAGCTGGG AGCCACTTCT GGAGATGATT CCAATAACAT TCCACCTATC AAAAGGGCAT 
TCTGAAATGG ATCCTGCTTT CTCTTTTGAT ATTTTAACTC AAAGAATCGC GGAAATTACA 
CTTTCTGCTA GGTCAATCGC TATGCTTTCT CACATCCCGG CGTCTCTAAC CGAAGAGCTG 
CCCTTAGCTT CCAGGGTATC TCAGAAGCCA TATCAACTGG TAAACGATAC AGAATTAGAT 
TTTGACGTCT GGATACAGGA TAAAACGACT GAAGACAACA AAAACGAAGT TGTGCTCTTA 
AAAGCAAACA CTTCTCTTCC TTGGGAATTT GAAGATTGGA GAAGTATTAG AGAGAAATTG 
GATATTGATA AGTCGAAGAA CATTTTGGGT GTTTGTGTTT CTGGGCAAAA TTATAAAACC 
ATTATGAATA TTGATGCCAC AACAGAAGGT GAAAACTTGC ATGTTTTGAG CCCACCAAGA 
AATAATGTGC ATAATAGAAT TGTTTGTGAG GCCAGATGTG ATGAGAATAA CGTGAAGATT 
ATTACGTTTA GGTCAACTTT AGTTATCGAA AACACAACTT CAACTGAGAT TGAGCTTTTG 
GTTGACTCCA AAGATCCAAA TAAGCCATCT TTAAAATATG CTATTAAACC ACATCAAAGT 
AAATCTGTTC CTGTAGAATA TGCCTACGAT TCTGATATTA GGATAAGACC CGCATCAGAG 
GATATATACG ATTGGTCTCA ACAGACGTTA TCTTGGAAAA GCTTGTTGAG CAACCAAATG 
TCTATATTCT GTTCTTCAAA AGAAGATTCC AATCAAAGAT TTCATTTCGA AATTGGTGCA 
AAGTATGATG AAAGAGAACC ATTGGCAAAG ATCTTTCCTC ATATGAAAAT CGTGGTTTCG 
GCATCTATGA CTATTGAAAA TTTACTTCCA GCAGATATTA ACTTCTCTAT TTTTGACAAA 
AGAGAGGAGA AGAGAACAGA TTTCTTGAAG ACTGGAGAAA GTATGGAAGT TCATCATATT 
TCTTTGGATA GTTTCCTGCT AATGTCTGTT CAACCATTAC AAGATGAGGC TTCTGCTTCA 
AAGCCCTCCA TCGTTAATAC ACCACATAAA AGTCCTTTAA ACCCAGAAGA TTCGTTGTCT 
TTGACCCTAT CTGGAGGTCA GAATTTGTTG TTGAAACTAG ATTATAAAAA CATCGATGGT 
ACAAGGTCCA AAGTTATTCG GATATACTCC CCTTATATTA TTATGAACTC TACCGATAGA 
GAACTCTACA TTCAAAGTAG TCTCTTAAAT ATTGCACAAT CCAAGATTTT GCTTGAAAAC 
GAAAAAAGAT ATACTATTCC AAAAATGTTT TCATTTGATA AAGAAGATGA TAAAAGTAAC 
AGAGCTCGTA TTAGGTTCAA GGAATCAGAG TGGAGTTCCA AATTATCATT TGACGCTATT 
GGCCAATCAT TTGATGCATC TGTTCGCATA AAAAATAAGG AACAAGAATC AAATTTAGGC 
ATCAACATTA GCGAAGGTAA AGGGAAGTAC TTGCTAAGTA AAGTTATTGA GATTGCACCT 
AGGTATATTA TAAGCAATAC TTTAGATATC CCTATTGAAG TTTGCGAGAC AGGGTCTATG 
GATGTCCAGC AAATAGAATC GAACATCACT AAGCCTTTAT ACAGGATGAG AAACATCGTC 
GACAAGCAAT TAGTGCTGAA ATTCTTAGGC GGAGACTCCA ATTGGTCACA ACCATTCTTT 
ATCAAAAATG TCGGTGTAAC ATATTTGAAA GTTTTGAAAA ATTCACGCCA TAAGCTTTTA 
AAGATTGAAA TTCTTTTGGA CAAAGCAACA ATATTCATAA GAATCAAGGA CGGTGGTGAC 
AGATGGCCAT TTTCCATAAG AAATTTCAGC GACCATGACT TTATATTTTA CCAAAGAGAT 
CCTCGTAAAG TTAGTGATCC ATACAAGGAT GACCAGTCTA ACGAGTCCTC AAGCCGTAGT 
TTCAAACCTA TCTTTTACAG GATTCCCTCG AAAAGTATTA TGCCATACGC ATGGGATTTT 
CCTACAGCTA AGGAGAAATA TCTGGTGTTA GAATCAGGTA CACGTACAAG GGAAGTTCGG 
TTGGCTGAGA TTGGCGAATT GCCACCGTTA CGCCTGGATA AAAGGTCAAA GGATAAACCA 
GCCCCCATTG TTGGATTACA TGTTGTTGCT GACGACGATA TGCAGGCCTT AGTTATTGTT 
AACTATAAGG CCAATGTTGG GTTATACAAA TTGAAAACTG CCTCTGCAAC TACCACGTCT 
TCAGTTTCAG TAAATAGTTC AGTGACTGAT GGCTTTGTGC AAAAGGATGA GGATGAGAAG 
GTTAATACAC AGATAGTTGT TTCGTTCAAA GGAGTTGGTA TCTCATTAAT CAATGGTCGT 
TTACAGGAAC TTCTATATAT TAACATGAGA GGGATCGAAT TAAGATACAA TGAATCTAAA 
GCATACCAGA CTTTCAGTTG GAAAATGAAA TGGATGCAAA TAGATAATCA ATTATTTTCA 
GGAAACTACT CAAATATTCT ATATCCAACA GAAATCCCCT ACACAGAAAA GGAAATTGAA 
AATCATCCAG TGATATCAGG TTCTATTTCT AAAGTCAACG ATTCCTTACA GGCTGTCCCA 
TACTTTAAAC ATGTTACGTT ATTAATCCAG GAATTTTCCA TACAATTAGA TGAAGACATG 
CTGTATGCTA TGATGGACTT CATAAAATTC CCAGGATCCC CATGGATCAT GGATTCAAGA 
GATTATAAGT ACGATGAAGA AATCCAGTTA CCTGATGTTT CTGAATTAAA AACGGCGGGT 
GATATATATT TCGAGATCTT CCATATTCAG CCCACCGTCT TACATCTGTC CTTTATACGC 
TCCGATGAAA TTAGTCCAGG ATTGGCTGAA GAGACCGAGG AGTCATTTTC GTCATCATTG 
TACTATGTCC ATATGTTTGC TATGACATTA GGTAATATAA ATGAGGCGCC GGTTAAAGTT 
AATTCTTTGT TCATGGATAA TGTAAGAGTC CCATTGCCCA TTCTGATGGA CCATATTGAG 
AGACACTATA CGACCCAGTT CGTGTATCAG ATCCACAAAA TTTTAGGATC AGCAGATTGT 
TTTGGTAATC CTGTAGGTCT TTTCAACACA ATTAGTTCCG GTGTCTGGGA CTTATTCTAT 
GAACCATATC AAGGTTACAT GATGAATGAT AGGCCTCAAG AAATTGGTAT ACATCTGGCT 
AAAGGTGGAC TAAGTTTTGC AAAGAAAACA GTCTTTGGCC TCTCTGATAG TATGTCCAAG 
TTTACGGGGT CGATGGCCAA GGGCCTGTCA GTTACACAAG ATCTGGAATT CCAAAGGGTC 
AGGAGATTAC AACAAAGAAT AAATAAAAAC AACAGGAATG CCTTGGCAAA CTCAGCGCAA 
TCCTTTGCCA GCACACTGGG AAGCGGGTTG AGCGGAATTG CACTGGACCC ATACAAGGCC 
ATGCAAAAGG AAGGCGCCGC TGGTTTCCTC AAAGGCCTTG GTAAAGGTAT CGTTGGGCTG 
CCAACTAAGA CAGCCATTGG ATTCTTAGAT CTTACAAGTA ACTTAAGTCA AGGCGTGAAA 
AGTACCACCA CTGTGCTTGA CATGCAAAAG GGTTGCAGAG TGCGTCTACC AAGGTACGTG 
GACCATGATC AAATCATCAA GCCTTATGAT CTACGTGAGG CCCAAGGACA ATACTGGCTC 
AAGACCGTGA ATGGAGGAGT ATTTATGAAT GATGAATACC TGTCGCACGT TATATTGCCA 
GGCAAAGAAC TAGCAGTAAT TGTGTCCATG CAGCACATTG CTGAGGTGCA AATGGCCACC 
CAAGAGCTTA TGTGGAGCAC AGGATATCCT TCCATCCAGG GAATTACATT GGAAAGATCC 
GGTCTTCAAA TTAAACTCAA GTCTCAATCC GAATACTTCA TTCCCATCAG CGATCCTGAA 
GAACGTCGGT CCTTGTACAG GAACATCGCC ATTGCTGTTA GAGAATACAA TAAGTACTGT 
GAAGCTATCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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VPS13 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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