Vps13a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps13a
Entrez Gene ID	271564
Full Name	vacuolar protein sorting 13A
Synonyms	4930425F11,4930516E05Rik,4930543C13Rik,9930023P20,Chac,Chorein,D330038K10Rik,mKIAA0986
General protein information	Preferred Names vacuolar protein sorting 13A Names vacuolar protein sorting-associated protein 13A chorea-acanthocytosis protein homolog vps13a
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A-B

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527126.4	XP_006527189.1	vacuolar protein sorting-associated protein 13A isoform X2
XM_017318216.2	XP_017173705.1	vacuolar protein sorting-associated protein 13A isoform X3
XM_011247256.2	XP_011245558.1	vacuolar protein sorting-associated protein 13A isoform X1
XM_006527126.3	XP_006527189.1	vacuolar protein sorting-associated protein 13A isoform X2
NM_173028.4	NP_766616.2	vacuolar protein sorting-associated protein 13A
XM_011247256.3	XP_011245558.1	vacuolar protein sorting-associated protein 13A isoform X1
XM_017318216.1	XP_017173705.1	vacuolar protein sorting-associated protein 13A isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS13A	NP_150648.2
	LOC705323	XP_002808150.1
Canis lupus familiaris (dog)	VPS13A	XP_533522.3
Bos taurus (cattle)	VPS13A	XP_002689716.3
Drosophila melanogaster (fruit fly)	Vps13	NP_001260781.1
Saccharomyces cerevisiae (baker's yeast)	VPS13	NP_013060.1
Eremothecium gossypii	AGOS_ACL064C	NP_983340.2
Magnaporthe oryzae (rice blast fungus)	MGG_06537	XP_003717004.1
Rattus norvegicus (Norway rat)	Vps13a	NP_001094445.2
Kluyveromyces lactis	KLLA0E00705g	XP_453978.1
Pan troglodytes (chimpanzee)	VPS13A	XP_001148547.1
Mus musculus (house mouse)	Vps13a	NP_766616.2
Xenopus tropicalis (tropical clawed frog)	vps13a	XP_002937242.2
Gallus gallus (chicken)	VPS13A	NP_001264765.1
Neurospora crassa	NCU05837	XP_960097.1
Danio rerio (zebrafish)	vps13a	NP_001112365.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005082	XP_558472.3
Caenorhabditis elegans (roundworm)	T08G11.1	NP_740900.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Phenotypic abnormalities in a chorea-acanthocytosis mouse model are modulated by strain background.
Sakimoto H, Nakamura M, Nagata O, Yokoyama I, Sano A
Biochemical and biophysical research communications472(1)118-24(2016 Mar)

Chorein, the protein responsible for chorea-acanthocytosis, interacts with ?-adducin and ?-actin.
Shiokawa N, Nakamura M, Sameshima M, Deguchi A, Hayashi T, Sasaki N, Sano A
Biochemical and biophysical research communications441(1)96-101(2013 Nov)

Subcellular localization and putative role of VPS13A/chorein in dopaminergic neuronal cells.
Hayashi T, Kishida M, Nishizawa Y, Iijima M, Koriyama C, Nakamura M, Sano A, Kishida S
Biochemical and biophysical research communications419(3)511-6(2012 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

chorein interacts with beta-adducin and beta-actin.
Title: Chorein, the protein responsible for chorea-acanthocytosis, interacts with ?-adducin and ?-actin.

Brain-specific variants of VPS13A may be involved in the brain-specific pathology of chorea-acanthocytosis.
Title: Brain-specific transcript variants of 5' and 3' ends of mouse VPS13A and VPS13C.

Mouse CHAC cDNA sequence and the exon-intron structures of the CHAC disease gene are identified and a CHAC model mouse produced by a gene-targeting technique.
Title: A gene-targeted mouse model for chorea-acanthocytosis.

The following Vps13a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps13a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu79978	XM_017318216.2 Latest version!	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu04438	NM_173028.4 Latest version!	Mus musculus vacuolar protein sorting 13A (Vps13a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu55584	XM_006527126.4 Latest version!	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu74479	XM_011247256.3 Latest version!	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMu55584	XM_006527126.3	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu74479	XM_011247256.2	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu79978	XM_017318216.1	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps13a ( XM_017318216.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173705.1 Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu79978
Clone ID Related Accession (Same CDS sequence)	XM_017318216.2 , XM_017318216.1
Accession Version	XM_017318216.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 13A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318216.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173705.1
CDS	303..6116
Translation	MVFESVVVEVLNRFLGDYVVNLDESQLSLGIWKGAVALKNLVIKENALHELDVPFKVKVGHIGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCLHDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKALKNGIVNENIVPEGYDFVFRPISASAKLQMNRRSDFDFSDPKINLAVDLHTIAIEFNKPQYFSLMELLESIDMMTQNQPYRKFKPSVPLHLHAKEWWAYAIHSILEVNVCPSLRMWSWEHIRNHRYKMKRYREFYKKKLTSKKPSPEILMSLEELEKTLDVFNITIARQQAEVEAKKAGYKIYKEGVKDPEDNAGWFGWLWTWSESNANQQQDVKPGILEEMLTPEEKSLLYEAIGYSETAVDPTLPKTFEALKFFVHLKSMSIVLRENHQKPELLNVVVEGLSTSVVQRPGAQAIKFETKIDSFHITGLPDDFKKPHLLSSLDDTSLLQITFEINPLNETVAQRCTIEAEPLEIIYDARTVNSIVEFFRPPKDVHLAQLTSVTLTKLEEFRAKTATGLLYVIETQKVLDLRINVKASYVIVPQYGNFSPTSNLLLLDLGHLKVSSKRRSLLPDVRPSEASLEDIMHRAYDSFDIQLTSIQLLYSRVGDNWKEARKLNVSTQHILIPMHVNVELSKAMVFMDIKMPKFKISGKLPLVSLRISDKKLQGIMELLGSIPKPEPVTDVSAPARSFQIQASALPVSHISQKLIPLLEQPVTEDDSEEEFFDAPCSPLEECPQVSCRDKCTRQKKLQKKDCVMNLIQLRMRFEVAEVSIQFYHLVGDCELPVLEMGALGLGTEAEFRTFDLKGSAFLKELWLKCPEYLDENKKPVYLITTLDNTMEDLLTLEFMKVEKNAPNLNSTYNNVLQLIKVNFSSLDIHLHTEALLNTMNYLNNILPELREKSASVSAAEPEDKGDIIKKLALKLPTNEDIITLQLLAELSCLQIFIQDQKQNISEIKIEGLDSEMIMKPLVTEINAKLRNIIVLDSDKMAIYKKALYITGKEVFSFKMISYMDATAGYAYTDMSVVDIRVHLTVGCIEVVFITKFLYSILAFIDNFQAVKDALAEATVQAAEMAADGVKELARKSSRFALDVNIKAPVVLIPQSPVSQNVFVADFGLITMKNIFVTVTETQSNIPPVIDLITIKLSKMRLYRSQFRNDTYQEVLDLLLPLNLEVIVERNLSWEWYKEVPCFNIKAQLKPMEFILSQEDLTTVFQTLHGNIWYGQDLSAPSSANKDPETMTSGVTSPPDHSPATVVTAAVVEVHPQASQAHTMLNVSFQTDYLTMALYSPGPDEASFTDVRDPSLELAEFKLENIISSLKIYTDDSTVFSFSVKNCILDDKRSHVMKATPRMIGLTVGFDKKDMVDIKYRKIKTFVVTDAVVQEMYVCASVEFLMTVAHIFFDAYMTSTALETSVQTRTTREAPAQELGKWEMNILIKNPEIVFVADMTRNDAPALVITTQCEICCKGEPTSNTVTAAIKDLQVRACPFLPVKRKGKVTTVLQPCDLFYQATQLGRDPQMIDISVKSLTLKVSPVIINTIITITSALYTTKETVPEENTSNIAHLWDKKDTKNLKMWFLEESNESEKVVPTNEVMPGGETLNLRIDSIFIVLEAGIGHRTVPMLLAKACFSGESKNWLSLINLHCHLELEVHYYNEMFGVWEPLLEPLEIDQTDDFRPWNLGIKMKKKAKEAIVESDSEAENYKVPEYKTAISFYSRDQLNITLSKCGLVMLNNLVEAFTEAATGSSSVFLRDLAPFMIFNSLGLTVSVSPSDSFSVLNVPLAKSYELKNDESLSMDYVRTKDNDHFNAMTSLSSKLFFILLN

Target ORF information:

RefSeq Version	XM_017318216.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318216.2

ORF Insert Sequence:

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps13a ( XM_017318216.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173705.1 Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X3, mRNA.

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CloneID	OMu79978
Clone ID Related Accession (Same CDS sequence)	XM_017318216.2 , XM_017318216.1
Accession Version	XM_017318216.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 13A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173705.1
CDS	1110..6923
Translation	MVFESVVVEVLNRFLGDYVVNLDESQLSLGIWKGAVALKNLVIKENALHELDVPFKVKVGHIGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCLHDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKALKNGIVNENIVPEGYDFVFRPISASAKLQMNRRSDFDFSDPKINLAVDLHTIAIEFNKPQYFSLMELLESIDMMTQNQPYRKFKPSVPLHLHAKEWWAYAIHSILEVNVCPSLRMWSWEHIRNHRYKMKRYREFYKKKLTSKKPSPEILMSLEELEKTLDVFNITIARQQAEVEAKKAGYKIYKEGVKDPEDNAGWFGWLWTWSESNANQQQDVKPGILEEMLTPEEKSLLYEAIGYSETAVDPTLPKTFEALKFFVHLKSMSIVLRENHQKPELLNVVVEGLSTSVVQRPGAQAIKFETKIDSFHITGLPDDFKKPHLLSSLDDTSLLQITFEINPLNETVAQRCTIEAEPLEIIYDARTVNSIVEFFRPPKDVHLAQLTSVTLTKLEEFRAKTATGLLYVIETQKVLDLRINVKASYVIVPQYGNFSPTSNLLLLDLGHLKVSSKRRSLLPDVRPSEASLEDIMHRAYDSFDIQLTSIQLLYSRVGDNWKEARKLNVSTQHILIPMHVNVELSKAMVFMDIKMPKFKISGKLPLVSLRISDKKLQGIMELLGSIPKPEPVTDVSAPARSFQIQASALPVSHISQKLIPLLEQPVTEDDSEEEFFDAPCSPLEECPQVSCRDKCTRQKKLQKKDCVMNLIQLRMRFEVAEVSIQFYHLVGDCELPVLEMGALGLGTEAEFRTFDLKGSAFLKELWLKCPEYLDENKKPVYLITTLDNTMEDLLTLEFMKVEKNAPNLNSTYNNVLQLIKVNFSSLDIHLHTEALLNTMNYLNNILPELREKSASVSAAEPEDKGDIIKKLALKLPTNEDIITLQLLAELSCLQIFIQDQKQNISEIKIEGLDSEMIMKPLVTEINAKLRNIIVLDSDKMAIYKKALYITGKEVFSFKMISYMDATAGYAYTDMSVVDIRVHLTVGCIEVVFITKFLYSILAFIDNFQAVKDALAEATVQAAEMAADGVKELARKSSRFALDVNIKAPVVLIPQSPVSQNVFVADFGLITMKNIFVTVTETQSNIPPVIDLITIKLSKMRLYRSQFRNDTYQEVLDLLLPLNLEVIVERNLSWEWYKEVPCFNIKAQLKPMEFILSQEDLTTVFQTLHGNIWYGQDLSAPSSANKDPETMTSGVTSPPDHSPATVVTAAVVEVHPQASQAHTMLNVSFQTDYLTMALYSPGPDEASFTDVRDPSLELAEFKLENIISSLKIYTDDSTVFSFSVKNCILDDKRSHVMKATPRMIGLTVGFDKKDMVDIKYRKIKTFVVTDAVVQEMYVCASVEFLMTVAHIFFDAYMTSTALETSVQTRTTREAPAQELGKWEMNILIKNPEIVFVADMTRNDAPALVITTQCEICCKGEPTSNTVTAAIKDLQVRACPFLPVKRKGKVTTVLQPCDLFYQATQLGRDPQMIDISVKSLTLKVSPVIINTIITITSALYTTKETVPEENTSNIAHLWDKKDTKNLKMWFLEESNESEKVVPTNEVMPGGETLNLRIDSIFIVLEAGIGHRTVPMLLAKACFSGESKNWLSLINLHCHLELEVHYYNEMFGVWEPLLEPLEIDQTDDFRPWNLGIKMKKKAKEAIVESDSEAENYKVPEYKTAISFYSRDQLNITLSKCGLVMLNNLVEAFTEAATGSSSVFLRDLAPFMIFNSLGLTVSVSPSDSFSVLNVPLAKSYELKNDESLSMDYVRTKDNDHFNAMTSLSSKLFFILLN

Target ORF information:

RefSeq Version	XM_017318216.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318216.1

ORF Insert Sequence:

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps13a ( NM_173028.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766616.2 Mus musculus vacuolar protein sorting 13A (Vps13a), mRNA.

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CloneID	OMu04438
Clone ID Related Accession (Same CDS sequence)	NM_173028.4
Accession Version	NM_173028.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-01
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 13A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125185.3, AB115421.1, BC062130.1, AK076578.1 and BE687829.1. On Nov 3, 2011 this sequence version replaced NM_173028.3. ##Evidence-Data-START## Transcript exon combination :: AB115421.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAC GCCTGCTAAC 
CACTCTGTCG CTGACAAGAT CCCTTTAACA AAAGTGGGAC GCCGTCTGTA CACTGTAAGA 
CACAGAGAAT CTGGGGTCGA AAGATCTATC ATTTGTCAAA TTGACACAGT TGAAGGAAGT 
AAGAAGGTAA CAATCCGCTC ACCAGTGCAG ATTAAGAATC ATTTTTCAAT CCCAATTTCC 
GTTTTTGAAG GAGATACTTT ATTGGGAATT GCTTCACCTG AAAATGAATT TAACATACCA 
TTAGCATCTT ACCGATCATC CCTTTCCCTG GTGCCAGAAG ACCAGGACTA CCAGCTATGT 
GAAGGAATCG ACTTTGAAGA AATCATAAAA TATGATGGAC AGCTTCTGAA GAAGAAATGT 
AGATCTACAA ACCCTTCTAA GAAATCATTT GTTATTAATA TTGTCCCAGA AAAGGATAAT 
TTGGCTTCCC TGTCAGTGTA TTCGGAAGAT GGTTGGGACT TACCATATGT CCTGCACTTG 
TGGCCACCCA TCCTCATCCG GAACCTTCTT CCCTACAAAG TTGCTTATTA CATTGAGGGC 
ATTGAGAACA CTGTTGTTAC TCTAAGTGAA GGACATTCAT CACAGATCTA TAATGTAGAA 
ATGGACCAAG CCAAGCTACA CTTAAAATTA CTCGACTATC TTAATCATGA TTGGAAAAGT 
GAATTTTATA TAAGATCTAG TCAACAAGAC ATCAATTTCA TCAACTTTAC CTGTCTCACA 
GAAATGGAAA AGAGTGACTT GGATATTGCT ATCCATATGA CTTACAACAC TGGTCAGACA 
GTTGTGGCAT TTCATAGTCC GTATTGGATG GTCAATAAAA CCAATCGGAT GCTACAGTAC 
AAAGCAGATG GAATTCACCG AAAGCATCCA CCTAATTATA CAAAACCAGT TCTCTTTTCC 
TTTCAGCCAA ATCACTTTTT TAACAACAAT AAGGTTCAGC TTATGGTAAC TGATAGTGAG 
CTTTCAGATC AGTTTTCAAT TGATACTGTT GGTAGTCATG GAGCCATTAG ATGCAAAGGT 
CTGAAAATGG AATACCAAGT TGGTGTCACT ATAAATCTCA GCAGTTTTAA CATTACCAGA 
ATTGTGACAT TTATTCCGTT TTATATGATT AAAAACAAAA GCAAATACCA TATATCAGTA 
GCAGAAGAAG GAAGTGATAA ATGGCTTTCT CTTGACTTAG AGCAGAGTAT CCCCTTTTGG 
CCTGAGAATG CCTCCAATAT TCTTCTTATT CAAGTTGAGA GGAGTGAAGA TCCTCCAAAA 
AGGATATATT TTAACAAGCA AGATAATTGT ATTTTATTGA GACTGAATAA TGAGCTTGGA 
GGTATTATTG CAGAAGTTAA TTTGGCTGAG CACTCTACAG TTATTACATT TTCAGATTAC 
CATGATGGAG CAGCTACATT CCTATTAATA AATCATACCA AGAGTGACCC TGTTCAGTAC 
AACCAAAGTT CTCTCGGTGA AATAGAAGAT TCTCTTCCTC CTGGAAAAGC AGTCTATTAT 
ACATGGGCTG ACCCAGTGGG CTCTAGAAAG CTGAAGTGGA GCTGTGGGCA AAGCTATGGT 
GAAGTAACCC ATAAGGATGA TATGATGACG CCCATAAGTG TGGGGAAAAA AACCATTTAT 
TTAGTGTCGT TCTTTGAAGG CCTGCAGCGT ATTATTTTAT TCACTGAAGA TCCAAGAGTG 
TTTAAAGTAA CGTATGAAAG TGAAAAGGCA GAGTTAGCGG AGCTGGAAGT TGTGCTGGCG 
TTACAGGATG TCGGGATTTC ACTTGTCAAT AACTATACAA AGCAAGAAGT AGCCTACATA 
GGCATTACAA GTTCTGATGT GGTTTGGGAG GCAAAACCAA AGAAGAAGGC CAGGTGGAAG 
CCCATGAGTG TGAAGCACAC AGAGAAGCTG GAGAAGGAGT TCAGGGAGTA CACAGAGGCC 
TCGCCCTTGG AGGACAAAGT GGTCGAGTTG GACAACATTC CGGTTCGTTT AACACCTAGT 
GGCAATGACA TGAAAATTCT GCAACCACAT GTGATACCTG TCCGGAGAAA TTACCTGCCA 
GCACTAAAAG TAGAATATAA CACATCTGCA CATCAGTCGT CATTCAGAAT TCAGATTTAT 
AGAATACAGA TCCAAAATCA GATTCATGGT GCTATTTTCC CTTTCGTGTT TTATCCTATT 
AAACCTCCAA GATCTGTCAC CATGGACTCA GCACCAAAGC CGTTTACAGA TGTCAGTATT 
GTCATGAGAT CTGCAGGACA TTCCCAAATA TCACGCATTA AGTATTTCAA AGTGTTGATT 
CAAGAAATGG ACCTCAGTTT GGATCTTGGA TTTGTGTATG CCCTAGCAGA CCTTGTCACA 
AAAGCTGAAG TGACTGAGAA GACAGAGGTG GAACATTTTC ATAAGGATGT AGAAGCATTT 
GAACAAGAAT ATGAAGTTGT TTCATCAGTA GATCAATCAC AAGTCAATCT CTTTGAATAT 
TTTCATATAT CTCCTATCAA GTTGCACTTG AGTGTTTCCC TGAGCTCTGG TAGAGATGAA 
GCGAAAGACT CAGAACAACA TGGAGGACTA ATTCCTGTTC ATTCGTTAAA TCTTTTGCTG 
AAGAGTATTG GTGCCACCCT CACAGATGTA CAAGATGTAG TTTTTAAGCT TGCCTTTTTT 
GAACTCAACT ACCAGTTCCA TACAACATCT GAATTGCAAT CTGAAGTAAT AAGACACTAT 
TCCAAACAGG CTATTAAACA AATGTATGTA CTCATTCTTG GACTTGACGT CTTGGGAAAT 
CCCTTTGGCT TAATTAGAGA ATTTTCAGAA GGAGTAGAAG CGTTTTTTTA TGAACCTTAC 
CAGGGGGCTA TCCAGGGTCC TGAAGAGTTT GTGGAGGGAA TGGCGCTAGG ATTGAAAGCA 
CTAGTTGGTG GAGCCGTTGG TGGGCTAGCT GGTGCTGCCT CCAAAATCAC CAGTGCTATG 
GCTAAAGGAG TAGCGGCCAT GACCATGGAT GAAGACTACC AGCAGAAGAG GAGAGAAGCA 
ATGAATAAGC AGCCTGCTGG CCTTCGAGAG GGCATCACTC GAGGAGGAAA AGGCCTTGTC 
TCTGGTTTTG TAAGTGGCAT TACAGGGATT GTTACAAAGC CAATTAAAGG TGCTCAAAAG 
GAAGGAGCAG CAGGTTTCTT CAAAGGTGTT GGAAAAGGTT TAGTTGGAGC AGTGACAAGG 
CCAACGGGTG GCATCATAGA CATGGCCAGC AGTACCTTTC AGGGAATCAA AAGAGCTACA 
GAGACTTCTG AAGTAGAGAG TTTACGACCT CCCAGATTCT TTAATGAAGA TGGAGTCATC 
AGACCTTACA GGTTGCGCGA TGGTTCAGGA AATCAAATGT TACAGGTCAT GGAAAATGGA 
AGATTTGCAA AATACAAGTA TTTTACCCAT GTCATGATCA ATAAAACAGA TATGTTCATG 
ATAACAAGAC GTGGCGTGTT GTTCGTAACA AAGGGAACAT TTGGACAGCT TACATGTGAG 
TGGCAGTACA CTTTTGATGA ATTCACCAAA GAGCCGTTCA TTGTCCATGG GAGAAGACTG 
CGCATTGAAG CCAAGGAACG GGTGAAGTCT GTATTCCATG CCAAAGAATT TGGAAAAATC 
GTTAACTTCA AGACTCCAGA AGATGCTAGG TGGATCCTCA CAAAGCTGGA AGAAGCGAGA 
GAACCATCCC CGAGACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766616.2
CDS	165..9665
Translation	MVFESVVVEVLNRFLGDYVVNLDESQLSLGIWKGAVALKNLVIKENALHELDVPFKVKVGHIGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCLHDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKALKNGIVNENIVPEGYDFVFRPISASAKLQMNRRSDFDFSDPKINLAVDLHTIAIEFNKPQYFSLMELLESIDMMTQNQPYRKFKPSVPLHLHAKEWWAYAIHSILEVNVCPSLRMWSWEHIRNHRYKMKRYREFYKKKLTSKKPSPEILMSLEELEKTLDVFNITIARQQAEVEAKKAGYKIYKEGVKDPEDNAGWFGWLWTWSESNANQQQDVKPGILEEMLTPEEKSLLYEAIGYSETAVDPTLPKTFEALKFFVHLKSMSIVLRENHQKPELLNVVVEGLSTSVVQRPGAQAIKFETKIDSFHITGLPDDFKKPHLLSSLDDTSLLQITFEINPLNETVAQRCTIEAEPLEIIYDARTVNSIVEFFRPPKDVHLAQLTSVTLTKLEEFRAKTATGLLYVIETQKVLDLRINVKASYVIVPQYGNFSPTSNLLLLDLGHLKVSSKRRSLLPDVRPSEASLEDIMHRAYDSFDIQLTSIQLLYSRVGDNWKEARKLNVSTQHILIPMHVNVELSKAMVFMDIKMPKFKISGKLPLVSLRISDKKLQGIMELLGSIPKPEPVTDVSAPARSFQIQASALPVSHISQKLIPLLEQPVTEDDSEEEFFDAPCSPLEECPQVSCRDKCTRQKKLQKKDCVMNLIQLRMRFEVAEVSIQFYHLVGDCELPVLEMGALGLGTEAEFRTFDLKGSAFLKELWLKCPEYLDENKKPVYLITTLDNTMEDLLTLEFMKVEKNAPNLNSTYNNVLQLIKVNFSSLDIHLHTEALLNTMNYLNNILPELREKSASVSAAEPEDKGDIIKKLALKLPTNEDIITLQLLAELSCLQIFIQDQKQNISEIKIEGLDSEMIMKPLVTEINAKLRNIIVLDSDKMAIYKKALYITGKEVFSFKMISYMDATAGYAYTDMSVVDIRVHLTVGCIEVVFITKFLYSILAFIDNFQAVKDALAEATVQAAEMAADGVKELARKSSRFALDVNIKAPVVLIPQSPVSQNVFVADFGLITMKNIFVTVTETQSNIPPVIDLITIKLSKMRLYRSQFRNDTYQEVLDLLLPLNLEVIVERNLSWEWYKEVPCFNIKAQLKPMEFILSQEDLTTVFQTLHGNIWYGQDLSAPSSANKDPETMTSGVTSPPDHSPATVVTAAVVEVHPQASQAHTMLNVSFQTDYLTMALYSPGPDEASFTDVRDPSLELAEFKLENIISSLKIYTDDSTVFSFSVKNCILDDKRSHVMKATPRMIGLTVGFDKKDMVDIKYRKIKTFVVTDAVVQEMYVCASVEFLMTVAHIFFDAYMTSTALETSVQTRTTREAPAQELGKWEMNILIKNPEIVFVADMTRNDAPALVITTQCEICCKGEPTSNTVTAAIKDLQVRACPFLPVKRKGKVTTVLQPCDLFYQATQLGRDPQMIDISVKSLTLKVSPVIINTIITITSALYTTKETVPEENTSNIAHLWDKKDTKNLKMWFLEESNESEKVVPTNEVMPGGETLNLRIDSIFIVLEAGIGHRTVPMLLAKACFSGESKNWLSLINLHCHLELEVHYYNEMFGVWEPLLEPLEIDQTDDFRPWNLGIKMKKKAKEAIVESDSEAENYKVPEYKTAISFYSRDQLNITLSKCGLVMLNNLVEAFTEAATGSSSVFLRDLAPFMIFNSLGLTVSVSPSDSFSVLNVPLAKSYELKNDESLSMDYVRTKDNDHFNAMTSLSSKLFFILLTPANHSVADKIPLTKVGRRLYTVRHRESGVERSIICQIDTVEGSKKVTIRSPVQIKNHFSIPISVFEGDTLLGIASPENEFNIPLASYRSSLSLVPEDQDYQLCEGIDFEEIIKYDGQLLKKKCRSTNPSKKSFVINIVPEKDNLASLSVYSEDGWDLPYVLHLWPPILIRNLLPYKVAYYIEGIENTVVTLSEGHSSQIYNVEMDQAKLHLKLLDYLNHDWKSEFYIRSSQQDINFINFTCLTEMEKSDLDIAIHMTYNTGQTVVAFHSPYWMVNKTNRMLQYKADGIHRKHPPNYTKPVLFSFQPNHFFNNNKVQLMVTDSELSDQFSIDTVGSHGAIRCKGLKMEYQVGVTINLSSFNITRIVTFIPFYMIKNKSKYHISVAEEGSDKWLSLDLEQSIPFWPENASNILLIQVERSEDPPKRIYFNKQDNCILLRLNNELGGIIAEVNLAEHSTVITFSDYHDGAATFLLINHTKSDPVQYNQSSLGEIEDSLPPGKAVYYTWADPVGSRKLKWSCGQSYGEVTHKDDMMTPISVGKKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAELEVVLALQDVGISLVNNYTKQEVAYIGITSSDVVWEAKPKKKARWKPMSVKHTEKLEKEFREYTEASPLEDKVVELDNIPVRLTPSGNDMKILQPHVIPVRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAIFPFVFYPIKPPRSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLSLDLGFVYALADLVTKAEVTEKTEVEHFHKDVEAFEQEYEVVSSVDQSQVNLFEYFHISPIKLHLSVSLSSGRDEAKDSEQHGGLIPVHSLNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSELQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITSAMAKGVAAMTMDEDYQQKRREAMNKQPAGLREGITRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRATETSEVESLRPPRFFNEDGVIRPYRLRDGSGNQMLQVMENGRFAKYKYFTHVMINKTDMFMITRRGVLFVTKGTFGQLTCEWQYTFDEFTKEPFIVHGRRLRIEAKERVKSVFHAKEFGKIVNFKTPEDARWILTKLEEAREPSPRL

Target ORF information:

RefSeq Version	NM_173028.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus vacuolar protein sorting 13A (Vps13a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173028.4

ORF Insert Sequence:

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAC GCCTGCTAAC 
CACTCTGTCG CTGACAAGAT CCCTTTAACA AAAGTGGGAC GCCGTCTGTA CACTGTAAGA 
CACAGAGAAT CTGGGGTCGA AAGATCTATC ATTTGTCAAA TTGACACAGT TGAAGGAAGT 
AAGAAGGTAA CAATCCGCTC ACCAGTGCAG ATTAAGAATC ATTTTTCAAT CCCAATTTCC 
GTTTTTGAAG GAGATACTTT ATTGGGAATT GCTTCACCTG AAAATGAATT TAACATACCA 
TTAGCATCTT ACCGATCATC CCTTTCCCTG GTGCCAGAAG ACCAGGACTA CCAGCTATGT 
GAAGGAATCG ACTTTGAAGA AATCATAAAA TATGATGGAC AGCTTCTGAA GAAGAAATGT 
AGATCTACAA ACCCTTCTAA GAAATCATTT GTTATTAATA TTGTCCCAGA AAAGGATAAT 
TTGGCTTCCC TGTCAGTGTA TTCGGAAGAT GGTTGGGACT TACCATATGT CCTGCACTTG 
TGGCCACCCA TCCTCATCCG GAACCTTCTT CCCTACAAAG TTGCTTATTA CATTGAGGGC 
ATTGAGAACA CTGTTGTTAC TCTAAGTGAA GGACATTCAT CACAGATCTA TAATGTAGAA 
ATGGACCAAG CCAAGCTACA CTTAAAATTA CTCGACTATC TTAATCATGA TTGGAAAAGT 
GAATTTTATA TAAGATCTAG TCAACAAGAC ATCAATTTCA TCAACTTTAC CTGTCTCACA 
GAAATGGAAA AGAGTGACTT GGATATTGCT ATCCATATGA CTTACAACAC TGGTCAGACA 
GTTGTGGCAT TTCATAGTCC GTATTGGATG GTCAATAAAA CCAATCGGAT GCTACAGTAC 
AAAGCAGATG GAATTCACCG AAAGCATCCA CCTAATTATA CAAAACCAGT TCTCTTTTCC 
TTTCAGCCAA ATCACTTTTT TAACAACAAT AAGGTTCAGC TTATGGTAAC TGATAGTGAG 
CTTTCAGATC AGTTTTCAAT TGATACTGTT GGTAGTCATG GAGCCATTAG ATGCAAAGGT 
CTGAAAATGG AATACCAAGT TGGTGTCACT ATAAATCTCA GCAGTTTTAA CATTACCAGA 
ATTGTGACAT TTATTCCGTT TTATATGATT AAAAACAAAA GCAAATACCA TATATCAGTA 
GCAGAAGAAG GAAGTGATAA ATGGCTTTCT CTTGACTTAG AGCAGAGTAT CCCCTTTTGG 
CCTGAGAATG CCTCCAATAT TCTTCTTATT CAAGTTGAGA GGAGTGAAGA TCCTCCAAAA 
AGGATATATT TTAACAAGCA AGATAATTGT ATTTTATTGA GACTGAATAA TGAGCTTGGA 
GGTATTATTG CAGAAGTTAA TTTGGCTGAG CACTCTACAG TTATTACATT TTCAGATTAC 
CATGATGGAG CAGCTACATT CCTATTAATA AATCATACCA AGAGTGACCC TGTTCAGTAC 
AACCAAAGTT CTCTCGGTGA AATAGAAGAT TCTCTTCCTC CTGGAAAAGC AGTCTATTAT 
ACATGGGCTG ACCCAGTGGG CTCTAGAAAG CTGAAGTGGA GCTGTGGGCA AAGCTATGGT 
GAAGTAACCC ATAAGGATGA TATGATGACG CCCATAAGTG TGGGGAAAAA AACCATTTAT 
TTAGTGTCGT TCTTTGAAGG CCTGCAGCGT ATTATTTTAT TCACTGAAGA TCCAAGAGTG 
TTTAAAGTAA CGTATGAAAG TGAAAAGGCA GAGTTAGCGG AGCTGGAAGT TGTGCTGGCG 
TTACAGGATG TCGGGATTTC ACTTGTCAAT AACTATACAA AGCAAGAAGT AGCCTACATA 
GGCATTACAA GTTCTGATGT GGTTTGGGAG GCAAAACCAA AGAAGAAGGC CAGGTGGAAG 
CCCATGAGTG TGAAGCACAC AGAGAAGCTG GAGAAGGAGT TCAGGGAGTA CACAGAGGCC 
TCGCCCTTGG AGGACAAAGT GGTCGAGTTG GACAACATTC CGGTTCGTTT AACACCTAGT 
GGCAATGACA TGAAAATTCT GCAACCACAT GTGATACCTG TCCGGAGAAA TTACCTGCCA 
GCACTAAAAG TAGAATATAA CACATCTGCA CATCAGTCGT CATTCAGAAT TCAGATTTAT 
AGAATACAGA TCCAAAATCA GATTCATGGT GCTATTTTCC CTTTCGTGTT TTATCCTATT 
AAACCTCCAA GATCTGTCAC CATGGACTCA GCACCAAAGC CGTTTACAGA TGTCAGTATT 
GTCATGAGAT CTGCAGGACA TTCCCAAATA TCACGCATTA AGTATTTCAA AGTGTTGATT 
CAAGAAATGG ACCTCAGTTT GGATCTTGGA TTTGTGTATG CCCTAGCAGA CCTTGTCACA 
AAAGCTGAAG TGACTGAGAA GACAGAGGTG GAACATTTTC ATAAGGATGT AGAAGCATTT 
GAACAAGAAT ATGAAGTTGT TTCATCAGTA GATCAATCAC AAGTCAATCT CTTTGAATAT 
TTTCATATAT CTCCTATCAA GTTGCACTTG AGTGTTTCCC TGAGCTCTGG TAGAGATGAA 
GCGAAAGACT CAGAACAACA TGGAGGACTA ATTCCTGTTC ATTCGTTAAA TCTTTTGCTG 
AAGAGTATTG GTGCCACCCT CACAGATGTA CAAGATGTAG TTTTTAAGCT TGCCTTTTTT 
GAACTCAACT ACCAGTTCCA TACAACATCT GAATTGCAAT CTGAAGTAAT AAGACACTAT 
TCCAAACAGG CTATTAAACA AATGTATGTA CTCATTCTTG GACTTGACGT CTTGGGAAAT 
CCCTTTGGCT TAATTAGAGA ATTTTCAGAA GGAGTAGAAG CGTTTTTTTA TGAACCTTAC 
CAGGGGGCTA TCCAGGGTCC TGAAGAGTTT GTGGAGGGAA TGGCGCTAGG ATTGAAAGCA 
CTAGTTGGTG GAGCCGTTGG TGGGCTAGCT GGTGCTGCCT CCAAAATCAC CAGTGCTATG 
GCTAAAGGAG TAGCGGCCAT GACCATGGAT GAAGACTACC AGCAGAAGAG GAGAGAAGCA 
ATGAATAAGC AGCCTGCTGG CCTTCGAGAG GGCATCACTC GAGGAGGAAA AGGCCTTGTC 
TCTGGTTTTG TAAGTGGCAT TACAGGGATT GTTACAAAGC CAATTAAAGG TGCTCAAAAG 
GAAGGAGCAG CAGGTTTCTT CAAAGGTGTT GGAAAAGGTT TAGTTGGAGC AGTGACAAGG 
CCAACGGGTG GCATCATAGA CATGGCCAGC AGTACCTTTC AGGGAATCAA AAGAGCTACA 
GAGACTTCTG AAGTAGAGAG TTTACGACCT CCCAGATTCT TTAATGAAGA TGGAGTCATC 
AGACCTTACA GGTTGCGCGA TGGTTCAGGA AATCAAATGT TACAGGTCAT GGAAAATGGA 
AGATTTGCAA AATACAAGTA TTTTACCCAT GTCATGATCA ATAAAACAGA TATGTTCATG 
ATAACAAGAC GTGGCGTGTT GTTCGTAACA AAGGGAACAT TTGGACAGCT TACATGTGAG 
TGGCAGTACA CTTTTGATGA ATTCACCAAA GAGCCGTTCA TTGTCCATGG GAGAAGACTG 
CGCATTGAAG CCAAGGAACG GGTGAAGTCT GTATTCCATG CCAAAGAATT TGGAAAAATC 
GTTAACTTCA AGACTCCAGA AGATGCTAGG TGGATCCTCA CAAAGCTGGA AGAAGCGAGA 
GAACCATCCC CGAGACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps13a ( XM_006527126.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527189.1 Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X2, mRNA.

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CloneID	OMu55584
Clone ID Related Accession (Same CDS sequence)	XM_006527126.4 , XM_006527126.3
Accession Version	XM_006527126.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 13A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527126.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAC GCCTGCTAAC 
CACTCTGTCG CTGACAAGAT CCCTTTAACA AAAGTGGGAC GCCGTCTGTA CACTGTAAGA 
CACAGAGAAT CTGGGGTCGA AAGATCTATC ATTTGTCAAA TTGACACAGT TGAAGGAAGT 
AAGAAGGTAA CAATCCGCTC ACCAGTGCAG ATTAAGAATC ATTTTTCAAT CCCAATTTCC 
GTTTTTGAAG GAGATACTTT ATTGGGAATT GCTTCACCTG AAAATGAATT TAACATACCA 
TTAGCATCTT ACCGATCATC CCTTTCCCTG GTGCCAGAAG ACCAGGACTA CCAGCTATGT 
GAAGGAATCG ACTTTGAAGA AATCATAAAA TATGATGGAC AGCTTCTGAA GAAGAAATGT 
AGATCTACAA ACCCTTCTAA GAAATCATTT GTTATTAATA TTGTCCCAGA AAAGGATAAT 
TTGGCTTCCC TGTCAGTGTA TTCGGAAGAT GGTTGGGACT TACCATATGT CCTGCACTTG 
TGGCCACCCA TCCTCATCCG GAACCTTCTT CCCTACAAAG TTGCTTATTA CATTGAGGGC 
ATTGAGAACA CTGTTGTTAC TCTAAGTGAA GGACATTCAT CACAGATCTA TAATGTAGAA 
ATGGACCAAG CCAAGCTACA CTTAAAATTA CTCGACTATC TTAATCATGA TTGGAAAAGT 
GAATTTTATA TAAGATCTAG TCAACAAGAC ATCAATTTCA TCAACTTTAC CTGTCTCACA 
GAAATGGAAA AGAGTGACTT GGATATTGCT ATCCATATGA CTTACAACAC TGGTCAGACA 
GTTGTGGCAT TTCATAGTCC GTATTGGATG GTCAATAAAA CCAATCGGAT GCTACAGTAC 
AAAGCAGATG GAATTCACCG AAAGCATCCA CCTAATTATA CAAAACCAGT TCTCTTTTCC 
TTTCAGCCAA ATCACTTTTT TAACAACAAT AAGGTTCAGC TTATGGTAAC TGATAGTGAG 
CTTTCAGATC AGTTTTCAAT TGATACTGTT GGTAGTCATG GAGCCATTAG ATGCAAAGGT 
CTGAAAATGG AATACCAAGT TGGTGTCACT ATAAATCTCA GCAGTTTTAA CATTACCAGA 
ATTGTGACAT TTATTCCGTT TTATATGATT AAAAACAAAA GCAAATACCA TATATCAGTA 
GCAGAAGAAG GAAGTGATAA ATGGCTTTCT CTTGACTTAG AGCAGAGTAT CCCCTTTTGG 
CCTGAGAATG CCTCCAATAT TCTTCTTATT CAAGTTGAGA GGAGTGAAGA TCCTCCAAAA 
AGGATATATT TTAACAAGCA AGATAATTGT ATTTTATTGA GACTGAATAA TGAGCTTGGA 
GGTATTATTG CAGAAGTTAA TTTGGCTGAG CACTCTACAG TTATTACATT TTCAGATTAC 
CATGATGGAG CAGCTACATT CCTATTAATA AATCATACCA AGAGTGACCC TGTTCAGTAC 
AACCAAAGTT CTCTCGGTGA AATAGAAGAT TCTCTTCCTC CTGGAAAAGC AGTCTATTAT 
ACATGGGCTG ACCCAGTGGG CTCTAGAAAG CTGAAGTGGA GCTGTGGGCA AAGCTATGGT 
GAAGTAACCC ATAAGGATGA TATGATGACG CCCATAAGTG TGGGGAAAAA AACCATTTAT 
TTAGTGTCGT TCTTTGAAGG CCTGCAGCGT ATTATTTTAT TCACTGAAGA TCCAAGAGTG 
TTTAAAGTAA CGTATGAAAG TGAAAAGGCA GAGTTAGCGG AGCTGGAAGT TGTGCTGGCG 
TTACAGGATG TCGGGATTTC ACTTGTCAAT AACTATACAA AGCAAGAAGT AGCCTACATA 
GGCATTACAA GTTCTGATGT GGTTTGGGAG GCAAAACCAA AGAAGAAGGC CAGGTGGAAG 
CCCATGAGTG TGAAGCACAC AGAGAAGCTG GAGAAGGAGT TCAGGGAGTA CACAGAGGCC 
TCGCCCTTGG AGGACAAAGT GGTCGAGTTG GACAACATTC CGGTTCGTTT AACACCTAGT 
GGCAATGACA TGAAAATTCT GCAACCACAT GTGATACCTG TCCGGAGAAA TTACCTGCCA 
GCACTAAAAG TAGAATATAA CACATCTGCA CATCAGTCGT CATTCAGAAT TCAGATTTAT 
AGAATACAGA TCCAAAATCA GATTCATGGT GCTATTTTCC CTTTCGTGTT TTATCCTATT 
AAACCTCCAA GATCTGTCAC CATGGACTCA GCACCAAAGC CGTTTACAGA TGTCAGTATT 
GTCATGAGAT CTGCAGGACA TTCCCAAATA TCACGCATTA AGTATTTCAA AGTGTTGATT 
CAAGAAATGG ACCTCAGTTT GGATCTTGGA TTTGTGTATG CCCTAGCAGA CCTTGTCACA 
AAAGCTGAAG TGACTGAGAA GACAGAGGTG GAACATTTTC ATAAGGATGT AGAAGCATTT 
GAACAAGAAT ATGAAGTTGT TTCATCAGTA GATCAATCAC AAGTCAATCT CTTTGAATAT 
TTTCATATAT CTCCTATCAA GTTGCACTTG AGTGTTTCCC TGAGCTCTGG TAGAGATGAA 
GCGAAAGACT CAGAACAACA TGGAGGACTA ATTCCTGTTC ATTCGTTAAA TCTTTTGCTG 
AAGAGTATTG GTGCCACCCT CACAGATGTA CAAGATGTAG TTTTTAAGCT TGCCTTTTTT 
GAACTCAACT ACCAGTTCCA TACAACATCT GAATTGCAAT CTGAAGTAAT AAGACACTAT 
TCCAAACAGG CTATTAAACA AATGTATGTA CTCATTCTTG GACTTGACGT CTTGGGAAAT 
CCCTTTGGCT TAATTAGAGA ATTTTCAGAA GGAGTAGAAG CGTTTTTTTA TGAACCTTAC 
CAGGGGGCTA TCCAGGGTCC TGAAGAGTTT GTGGAGGGAA TGGCGCTAGG ATTGAAAGCA 
CTAGTTGGTG GAGCCGTTGG TGGGCTAGCT GGTGCTGCCT CCAAAATCAC CAGTGCTATG 
GCTAAAGGAG TAGCGGCCAT GACCATGGAT GAAGACTACC AGCAGAAGAG GAGAGAAGCA 
ATGAATAAGC AGCCTGCTGG CCTTCGAGAG GGCATCACTC GAGGAGGAAA AGGCCTTGTC 
TCTGGTTTTG TAAGTGGCAT TACAGGGATT GTTACAAAGC CAATTAAAGG TGCTCAAAAG 
GAAGGAGCAG CAGGTTTCTT CAAAGGTGTT GGAAAAGGTT TAGTTGGAGC AGTGACAAGG 
CCAACGGGTG GCATCATAGA CATGGCCAGC AGTACCTTTC AGGGAATCAA AAGAGCTACA 
GAGACTTCTG AAGTAGAGAG TTTACGACCT CCCAGATTCT TTAATGAAGA TGGAGTCATC 
AGACCTTACA GGTTGCGCGA TGGTTCAGGA AATCAAATGT TACAGGCATC AAAAAGTTTG 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527189.1
CDS	301..9486
Translation	MVFESVVVEVLNRFLGDYVVNLDESQLSLGIWKGAVALKNLVIKENALHELDVPFKVKVGHIGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCLHDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKALKNGIVNENIVPEGYDFVFRPISASAKLQMNRRSDFDFSDPKINLAVDLHTIAIEFNKPQYFSLMELLESIDMMTQNQPYRKFKPSVPLHLHAKEWWAYAIHSILEVNVCPSLRMWSWEHIRNHRYKMKRYREFYKKKLTSKKPSPEILMSLEELEKTLDVFNITIARQQAEVEAKKAGYKIYKEGVKDPEDNAGWFGWLWTWSESNANQQQDVKPGILEEMLTPEEKSLLYEAIGYSETAVDPTLPKTFEALKFFVHLKSMSIVLRENHQKPELLNVVVEGLSTSVVQRPGAQAIKFETKIDSFHITGLPDDFKKPHLLSSLDDTSLLQITFEINPLNETVAQRCTIEAEPLEIIYDARTVNSIVEFFRPPKDVHLAQLTSVTLTKLEEFRAKTATGLLYVIETQKVLDLRINVKASYVIVPQYGNFSPTSNLLLLDLGHLKVSSKRRSLLPDVRPSEASLEDIMHRAYDSFDIQLTSIQLLYSRVGDNWKEARKLNVSTQHILIPMHVNVELSKAMVFMDIKMPKFKISGKLPLVSLRISDKKLQGIMELLGSIPKPEPVTDVSAPARSFQIQASALPVSHISQKLIPLLEQPVTEDDSEEEFFDAPCSPLEECPQVSCRDKCTRQKKLQKKDCVMNLIQLRMRFEVAEVSIQFYHLVGDCELPVLEMGALGLGTEAEFRTFDLKGSAFLKELWLKCPEYLDENKKPVYLITTLDNTMEDLLTLEFMKVEKNAPNLNSTYNNVLQLIKVNFSSLDIHLHTEALLNTMNYLNNILPELREKSASVSAAEPEDKGDIIKKLALKLPTNEDIITLQLLAELSCLQIFIQDQKQNISEIKIEGLDSEMIMKPLVTEINAKLRNIIVLDSDKMAIYKKALYITGKEVFSFKMISYMDATAGYAYTDMSVVDIRVHLTVGCIEVVFITKFLYSILAFIDNFQAVKDALAEATVQAAEMAADGVKELARKSSRFALDVNIKAPVVLIPQSPVSQNVFVADFGLITMKNIFVTVTETQSNIPPVIDLITIKLSKMRLYRSQFRNDTYQEVLDLLLPLNLEVIVERNLSWEWYKEVPCFNIKAQLKPMEFILSQEDLTTVFQTLHGNIWYGQDLSAPSSANKDPETMTSGVTSPPDHSPATVVTAAVVEVHPQASQAHTMLNVSFQTDYLTMALYSPGPDEASFTDVRDPSLELAEFKLENIISSLKIYTDDSTVFSFSVKNCILDDKRSHVMKATPRMIGLTVGFDKKDMVDIKYRKIKTFVVTDAVVQEMYVCASVEFLMTVAHIFFDAYMTSTALETSVQTRTTREAPAQELGKWEMNILIKNPEIVFVADMTRNDAPALVITTQCEICCKGEPTSNTVTAAIKDLQVRACPFLPVKRKGKVTTVLQPCDLFYQATQLGRDPQMIDISVKSLTLKVSPVIINTIITITSALYTTKETVPEENTSNIAHLWDKKDTKNLKMWFLEESNESEKVVPTNEVMPGGETLNLRIDSIFIVLEAGIGHRTVPMLLAKACFSGESKNWLSLINLHCHLELEVHYYNEMFGVWEPLLEPLEIDQTDDFRPWNLGIKMKKKAKEAIVESDSEAENYKVPEYKTAISFYSRDQLNITLSKCGLVMLNNLVEAFTEAATGSSSVFLRDLAPFMIFNSLGLTVSVSPSDSFSVLNVPLAKSYELKNDESLSMDYVRTKDNDHFNAMTSLSSKLFFILLTPANHSVADKIPLTKVGRRLYTVRHRESGVERSIICQIDTVEGSKKVTIRSPVQIKNHFSIPISVFEGDTLLGIASPENEFNIPLASYRSSLSLVPEDQDYQLCEGIDFEEIIKYDGQLLKKKCRSTNPSKKSFVINIVPEKDNLASLSVYSEDGWDLPYVLHLWPPILIRNLLPYKVAYYIEGIENTVVTLSEGHSSQIYNVEMDQAKLHLKLLDYLNHDWKSEFYIRSSQQDINFINFTCLTEMEKSDLDIAIHMTYNTGQTVVAFHSPYWMVNKTNRMLQYKADGIHRKHPPNYTKPVLFSFQPNHFFNNNKVQLMVTDSELSDQFSIDTVGSHGAIRCKGLKMEYQVGVTINLSSFNITRIVTFIPFYMIKNKSKYHISVAEEGSDKWLSLDLEQSIPFWPENASNILLIQVERSEDPPKRIYFNKQDNCILLRLNNELGGIIAEVNLAEHSTVITFSDYHDGAATFLLINHTKSDPVQYNQSSLGEIEDSLPPGKAVYYTWADPVGSRKLKWSCGQSYGEVTHKDDMMTPISVGKKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAELEVVLALQDVGISLVNNYTKQEVAYIGITSSDVVWEAKPKKKARWKPMSVKHTEKLEKEFREYTEASPLEDKVVELDNIPVRLTPSGNDMKILQPHVIPVRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAIFPFVFYPIKPPRSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLSLDLGFVYALADLVTKAEVTEKTEVEHFHKDVEAFEQEYEVVSSVDQSQVNLFEYFHISPIKLHLSVSLSSGRDEAKDSEQHGGLIPVHSLNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSELQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITSAMAKGVAAMTMDEDYQQKRREAMNKQPAGLREGITRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRATETSEVESLRPPRFFNEDGVIRPYRLRDGSGNQMLQASKSLI

Target ORF information:

RefSeq Version	XM_006527126.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527126.4

ORF Insert Sequence:

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAC GCCTGCTAAC 
CACTCTGTCG CTGACAAGAT CCCTTTAACA AAAGTGGGAC GCCGTCTGTA CACTGTAAGA 
CACAGAGAAT CTGGGGTCGA AAGATCTATC ATTTGTCAAA TTGACACAGT TGAAGGAAGT 
AAGAAGGTAA CAATCCGCTC ACCAGTGCAG ATTAAGAATC ATTTTTCAAT CCCAATTTCC 
GTTTTTGAAG GAGATACTTT ATTGGGAATT GCTTCACCTG AAAATGAATT TAACATACCA 
TTAGCATCTT ACCGATCATC CCTTTCCCTG GTGCCAGAAG ACCAGGACTA CCAGCTATGT 
GAAGGAATCG ACTTTGAAGA AATCATAAAA TATGATGGAC AGCTTCTGAA GAAGAAATGT 
AGATCTACAA ACCCTTCTAA GAAATCATTT GTTATTAATA TTGTCCCAGA AAAGGATAAT 
TTGGCTTCCC TGTCAGTGTA TTCGGAAGAT GGTTGGGACT TACCATATGT CCTGCACTTG 
TGGCCACCCA TCCTCATCCG GAACCTTCTT CCCTACAAAG TTGCTTATTA CATTGAGGGC 
ATTGAGAACA CTGTTGTTAC TCTAAGTGAA GGACATTCAT CACAGATCTA TAATGTAGAA 
ATGGACCAAG CCAAGCTACA CTTAAAATTA CTCGACTATC TTAATCATGA TTGGAAAAGT 
GAATTTTATA TAAGATCTAG TCAACAAGAC ATCAATTTCA TCAACTTTAC CTGTCTCACA 
GAAATGGAAA AGAGTGACTT GGATATTGCT ATCCATATGA CTTACAACAC TGGTCAGACA 
GTTGTGGCAT TTCATAGTCC GTATTGGATG GTCAATAAAA CCAATCGGAT GCTACAGTAC 
AAAGCAGATG GAATTCACCG AAAGCATCCA CCTAATTATA CAAAACCAGT TCTCTTTTCC 
TTTCAGCCAA ATCACTTTTT TAACAACAAT AAGGTTCAGC TTATGGTAAC TGATAGTGAG 
CTTTCAGATC AGTTTTCAAT TGATACTGTT GGTAGTCATG GAGCCATTAG ATGCAAAGGT 
CTGAAAATGG AATACCAAGT TGGTGTCACT ATAAATCTCA GCAGTTTTAA CATTACCAGA 
ATTGTGACAT TTATTCCGTT TTATATGATT AAAAACAAAA GCAAATACCA TATATCAGTA 
GCAGAAGAAG GAAGTGATAA ATGGCTTTCT CTTGACTTAG AGCAGAGTAT CCCCTTTTGG 
CCTGAGAATG CCTCCAATAT TCTTCTTATT CAAGTTGAGA GGAGTGAAGA TCCTCCAAAA 
AGGATATATT TTAACAAGCA AGATAATTGT ATTTTATTGA GACTGAATAA TGAGCTTGGA 
GGTATTATTG CAGAAGTTAA TTTGGCTGAG CACTCTACAG TTATTACATT TTCAGATTAC 
CATGATGGAG CAGCTACATT CCTATTAATA AATCATACCA AGAGTGACCC TGTTCAGTAC 
AACCAAAGTT CTCTCGGTGA AATAGAAGAT TCTCTTCCTC CTGGAAAAGC AGTCTATTAT 
ACATGGGCTG ACCCAGTGGG CTCTAGAAAG CTGAAGTGGA GCTGTGGGCA AAGCTATGGT 
GAAGTAACCC ATAAGGATGA TATGATGACG CCCATAAGTG TGGGGAAAAA AACCATTTAT 
TTAGTGTCGT TCTTTGAAGG CCTGCAGCGT ATTATTTTAT TCACTGAAGA TCCAAGAGTG 
TTTAAAGTAA CGTATGAAAG TGAAAAGGCA GAGTTAGCGG AGCTGGAAGT TGTGCTGGCG 
TTACAGGATG TCGGGATTTC ACTTGTCAAT AACTATACAA AGCAAGAAGT AGCCTACATA 
GGCATTACAA GTTCTGATGT GGTTTGGGAG GCAAAACCAA AGAAGAAGGC CAGGTGGAAG 
CCCATGAGTG TGAAGCACAC AGAGAAGCTG GAGAAGGAGT TCAGGGAGTA CACAGAGGCC 
TCGCCCTTGG AGGACAAAGT GGTCGAGTTG GACAACATTC CGGTTCGTTT AACACCTAGT 
GGCAATGACA TGAAAATTCT GCAACCACAT GTGATACCTG TCCGGAGAAA TTACCTGCCA 
GCACTAAAAG TAGAATATAA CACATCTGCA CATCAGTCGT CATTCAGAAT TCAGATTTAT 
AGAATACAGA TCCAAAATCA GATTCATGGT GCTATTTTCC CTTTCGTGTT TTATCCTATT 
AAACCTCCAA GATCTGTCAC CATGGACTCA GCACCAAAGC CGTTTACAGA TGTCAGTATT 
GTCATGAGAT CTGCAGGACA TTCCCAAATA TCACGCATTA AGTATTTCAA AGTGTTGATT 
CAAGAAATGG ACCTCAGTTT GGATCTTGGA TTTGTGTATG CCCTAGCAGA CCTTGTCACA 
AAAGCTGAAG TGACTGAGAA GACAGAGGTG GAACATTTTC ATAAGGATGT AGAAGCATTT 
GAACAAGAAT ATGAAGTTGT TTCATCAGTA GATCAATCAC AAGTCAATCT CTTTGAATAT 
TTTCATATAT CTCCTATCAA GTTGCACTTG AGTGTTTCCC TGAGCTCTGG TAGAGATGAA 
GCGAAAGACT CAGAACAACA TGGAGGACTA ATTCCTGTTC ATTCGTTAAA TCTTTTGCTG 
AAGAGTATTG GTGCCACCCT CACAGATGTA CAAGATGTAG TTTTTAAGCT TGCCTTTTTT 
GAACTCAACT ACCAGTTCCA TACAACATCT GAATTGCAAT CTGAAGTAAT AAGACACTAT 
TCCAAACAGG CTATTAAACA AATGTATGTA CTCATTCTTG GACTTGACGT CTTGGGAAAT 
CCCTTTGGCT TAATTAGAGA ATTTTCAGAA GGAGTAGAAG CGTTTTTTTA TGAACCTTAC 
CAGGGGGCTA TCCAGGGTCC TGAAGAGTTT GTGGAGGGAA TGGCGCTAGG ATTGAAAGCA 
CTAGTTGGTG GAGCCGTTGG TGGGCTAGCT GGTGCTGCCT CCAAAATCAC CAGTGCTATG 
GCTAAAGGAG TAGCGGCCAT GACCATGGAT GAAGACTACC AGCAGAAGAG GAGAGAAGCA 
ATGAATAAGC AGCCTGCTGG CCTTCGAGAG GGCATCACTC GAGGAGGAAA AGGCCTTGTC 
TCTGGTTTTG TAAGTGGCAT TACAGGGATT GTTACAAAGC CAATTAAAGG TGCTCAAAAG 
GAAGGAGCAG CAGGTTTCTT CAAAGGTGTT GGAAAAGGTT TAGTTGGAGC AGTGACAAGG 
CCAACGGGTG GCATCATAGA CATGGCCAGC AGTACCTTTC AGGGAATCAA AAGAGCTACA 
GAGACTTCTG AAGTAGAGAG TTTACGACCT CCCAGATTCT TTAATGAAGA TGGAGTCATC 
AGACCTTACA GGTTGCGCGA TGGTTCAGGA AATCAAATGT TACAGGCATC AAAAAGTTTG 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps13a ( XM_006527126.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527189.1 Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X2, mRNA.

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CloneID	OMu55584
Clone ID Related Accession (Same CDS sequence)	XM_006527126.4 , XM_006527126.3
Accession Version	XM_006527126.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 13A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527126.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAC GCCTGCTAAC 
CACTCTGTCG CTGACAAGAT CCCTTTAACA AAAGTGGGAC GCCGTCTGTA CACTGTAAGA 
CACAGAGAAT CTGGGGTCGA AAGATCTATC ATTTGTCAAA TTGACACAGT TGAAGGAAGT 
AAGAAGGTAA CAATCCGCTC ACCAGTGCAG ATTAAGAATC ATTTTTCAAT CCCAATTTCC 
GTTTTTGAAG GAGATACTTT ATTGGGAATT GCTTCACCTG AAAATGAATT TAACATACCA 
TTAGCATCTT ACCGATCATC CCTTTCCCTG GTGCCAGAAG ACCAGGACTA CCAGCTATGT 
GAAGGAATCG ACTTTGAAGA AATCATAAAA TATGATGGAC AGCTTCTGAA GAAGAAATGT 
AGATCTACAA ACCCTTCTAA GAAATCATTT GTTATTAATA TTGTCCCAGA AAAGGATAAT 
TTGGCTTCCC TGTCAGTGTA TTCGGAAGAT GGTTGGGACT TACCATATGT CCTGCACTTG 
TGGCCACCCA TCCTCATCCG GAACCTTCTT CCCTACAAAG TTGCTTATTA CATTGAGGGC 
ATTGAGAACA CTGTTGTTAC TCTAAGTGAA GGACATTCAT CACAGATCTA TAATGTAGAA 
ATGGACCAAG CCAAGCTACA CTTAAAATTA CTCGACTATC TTAATCATGA TTGGAAAAGT 
GAATTTTATA TAAGATCTAG TCAACAAGAC ATCAATTTCA TCAACTTTAC CTGTCTCACA 
GAAATGGAAA AGAGTGACTT GGATATTGCT ATCCATATGA CTTACAACAC TGGTCAGACA 
GTTGTGGCAT TTCATAGTCC GTATTGGATG GTCAATAAAA CCAATCGGAT GCTACAGTAC 
AAAGCAGATG GAATTCACCG AAAGCATCCA CCTAATTATA CAAAACCAGT TCTCTTTTCC 
TTTCAGCCAA ATCACTTTTT TAACAACAAT AAGGTTCAGC TTATGGTAAC TGATAGTGAG 
CTTTCAGATC AGTTTTCAAT TGATACTGTT GGTAGTCATG GAGCCATTAG ATGCAAAGGT 
CTGAAAATGG AATACCAAGT TGGTGTCACT ATAAATCTCA GCAGTTTTAA CATTACCAGA 
ATTGTGACAT TTATTCCGTT TTATATGATT AAAAACAAAA GCAAATACCA TATATCAGTA 
GCAGAAGAAG GAAGTGATAA ATGGCTTTCT CTTGACTTAG AGCAGAGTAT CCCCTTTTGG 
CCTGAGAATG CCTCCAATAT TCTTCTTATT CAAGTTGAGA GGAGTGAAGA TCCTCCAAAA 
AGGATATATT TTAACAAGCA AGATAATTGT ATTTTATTGA GACTGAATAA TGAGCTTGGA 
GGTATTATTG CAGAAGTTAA TTTGGCTGAG CACTCTACAG TTATTACATT TTCAGATTAC 
CATGATGGAG CAGCTACATT CCTATTAATA AATCATACCA AGAGTGACCC TGTTCAGTAC 
AACCAAAGTT CTCTCGGTGA AATAGAAGAT TCTCTTCCTC CTGGAAAAGC AGTCTATTAT 
ACATGGGCTG ACCCAGTGGG CTCTAGAAAG CTGAAGTGGA GCTGTGGGCA AAGCTATGGT 
GAAGTAACCC ATAAGGATGA TATGATGACG CCCATAAGTG TGGGGAAAAA AACCATTTAT 
TTAGTGTCGT TCTTTGAAGG CCTGCAGCGT ATTATTTTAT TCACTGAAGA TCCAAGAGTG 
TTTAAAGTAA CGTATGAAAG TGAAAAGGCA GAGTTAGCGG AGCTGGAAGT TGTGCTGGCG 
TTACAGGATG TCGGGATTTC ACTTGTCAAT AACTATACAA AGCAAGAAGT AGCCTACATA 
GGCATTACAA GTTCTGATGT GGTTTGGGAG GCAAAACCAA AGAAGAAGGC CAGGTGGAAG 
CCCATGAGTG TGAAGCACAC AGAGAAGCTG GAGAAGGAGT TCAGGGAGTA CACAGAGGCC 
TCGCCCTTGG AGGACAAAGT GGTCGAGTTG GACAACATTC CGGTTCGTTT AACACCTAGT 
GGCAATGACA TGAAAATTCT GCAACCACAT GTGATACCTG TCCGGAGAAA TTACCTGCCA 
GCACTAAAAG TAGAATATAA CACATCTGCA CATCAGTCGT CATTCAGAAT TCAGATTTAT 
AGAATACAGA TCCAAAATCA GATTCATGGT GCTATTTTCC CTTTCGTGTT TTATCCTATT 
AAACCTCCAA GATCTGTCAC CATGGACTCA GCACCAAAGC CGTTTACAGA TGTCAGTATT 
GTCATGAGAT CTGCAGGACA TTCCCAAATA TCACGCATTA AGTATTTCAA AGTGTTGATT 
CAAGAAATGG ACCTCAGTTT GGATCTTGGA TTTGTGTATG CCCTAGCAGA CCTTGTCACA 
AAAGCTGAAG TGACTGAGAA GACAGAGGTG GAACATTTTC ATAAGGATGT AGAAGCATTT 
GAACAAGAAT ATGAAGTTGT TTCATCAGTA GATCAATCAC AAGTCAATCT CTTTGAATAT 
TTTCATATAT CTCCTATCAA GTTGCACTTG AGTGTTTCCC TGAGCTCTGG TAGAGATGAA 
GCGAAAGACT CAGAACAACA TGGAGGACTA ATTCCTGTTC ATTCGTTAAA TCTTTTGCTG 
AAGAGTATTG GTGCCACCCT CACAGATGTA CAAGATGTAG TTTTTAAGCT TGCCTTTTTT 
GAACTCAACT ACCAGTTCCA TACAACATCT GAATTGCAAT CTGAAGTAAT AAGACACTAT 
TCCAAACAGG CTATTAAACA AATGTATGTA CTCATTCTTG GACTTGACGT CTTGGGAAAT 
CCCTTTGGCT TAATTAGAGA ATTTTCAGAA GGAGTAGAAG CGTTTTTTTA TGAACCTTAC 
CAGGGGGCTA TCCAGGGTCC TGAAGAGTTT GTGGAGGGAA TGGCGCTAGG ATTGAAAGCA 
CTAGTTGGTG GAGCCGTTGG TGGGCTAGCT GGTGCTGCCT CCAAAATCAC CAGTGCTATG 
GCTAAAGGAG TAGCGGCCAT GACCATGGAT GAAGACTACC AGCAGAAGAG GAGAGAAGCA 
ATGAATAAGC AGCCTGCTGG CCTTCGAGAG GGCATCACTC GAGGAGGAAA AGGCCTTGTC 
TCTGGTTTTG TAAGTGGCAT TACAGGGATT GTTACAAAGC CAATTAAAGG TGCTCAAAAG 
GAAGGAGCAG CAGGTTTCTT CAAAGGTGTT GGAAAAGGTT TAGTTGGAGC AGTGACAAGG 
CCAACGGGTG GCATCATAGA CATGGCCAGC AGTACCTTTC AGGGAATCAA AAGAGCTACA 
GAGACTTCTG AAGTAGAGAG TTTACGACCT CCCAGATTCT TTAATGAAGA TGGAGTCATC 
AGACCTTACA GGTTGCGCGA TGGTTCAGGA AATCAAATGT TACAGGCATC AAAAAGTTTG 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527189.1
CDS	1098..10283
Translation	MVFESVVVEVLNRFLGDYVVNLDESQLSLGIWKGAVALKNLVIKENALHELDVPFKVKVGHIGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCLHDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKALKNGIVNENIVPEGYDFVFRPISASAKLQMNRRSDFDFSDPKINLAVDLHTIAIEFNKPQYFSLMELLESIDMMTQNQPYRKFKPSVPLHLHAKEWWAYAIHSILEVNVCPSLRMWSWEHIRNHRYKMKRYREFYKKKLTSKKPSPEILMSLEELEKTLDVFNITIARQQAEVEAKKAGYKIYKEGVKDPEDNAGWFGWLWTWSESNANQQQDVKPGILEEMLTPEEKSLLYEAIGYSETAVDPTLPKTFEALKFFVHLKSMSIVLRENHQKPELLNVVVEGLSTSVVQRPGAQAIKFETKIDSFHITGLPDDFKKPHLLSSLDDTSLLQITFEINPLNETVAQRCTIEAEPLEIIYDARTVNSIVEFFRPPKDVHLAQLTSVTLTKLEEFRAKTATGLLYVIETQKVLDLRINVKASYVIVPQYGNFSPTSNLLLLDLGHLKVSSKRRSLLPDVRPSEASLEDIMHRAYDSFDIQLTSIQLLYSRVGDNWKEARKLNVSTQHILIPMHVNVELSKAMVFMDIKMPKFKISGKLPLVSLRISDKKLQGIMELLGSIPKPEPVTDVSAPARSFQIQASALPVSHISQKLIPLLEQPVTEDDSEEEFFDAPCSPLEECPQVSCRDKCTRQKKLQKKDCVMNLIQLRMRFEVAEVSIQFYHLVGDCELPVLEMGALGLGTEAEFRTFDLKGSAFLKELWLKCPEYLDENKKPVYLITTLDNTMEDLLTLEFMKVEKNAPNLNSTYNNVLQLIKVNFSSLDIHLHTEALLNTMNYLNNILPELREKSASVSAAEPEDKGDIIKKLALKLPTNEDIITLQLLAELSCLQIFIQDQKQNISEIKIEGLDSEMIMKPLVTEINAKLRNIIVLDSDKMAIYKKALYITGKEVFSFKMISYMDATAGYAYTDMSVVDIRVHLTVGCIEVVFITKFLYSILAFIDNFQAVKDALAEATVQAAEMAADGVKELARKSSRFALDVNIKAPVVLIPQSPVSQNVFVADFGLITMKNIFVTVTETQSNIPPVIDLITIKLSKMRLYRSQFRNDTYQEVLDLLLPLNLEVIVERNLSWEWYKEVPCFNIKAQLKPMEFILSQEDLTTVFQTLHGNIWYGQDLSAPSSANKDPETMTSGVTSPPDHSPATVVTAAVVEVHPQASQAHTMLNVSFQTDYLTMALYSPGPDEASFTDVRDPSLELAEFKLENIISSLKIYTDDSTVFSFSVKNCILDDKRSHVMKATPRMIGLTVGFDKKDMVDIKYRKIKTFVVTDAVVQEMYVCASVEFLMTVAHIFFDAYMTSTALETSVQTRTTREAPAQELGKWEMNILIKNPEIVFVADMTRNDAPALVITTQCEICCKGEPTSNTVTAAIKDLQVRACPFLPVKRKGKVTTVLQPCDLFYQATQLGRDPQMIDISVKSLTLKVSPVIINTIITITSALYTTKETVPEENTSNIAHLWDKKDTKNLKMWFLEESNESEKVVPTNEVMPGGETLNLRIDSIFIVLEAGIGHRTVPMLLAKACFSGESKNWLSLINLHCHLELEVHYYNEMFGVWEPLLEPLEIDQTDDFRPWNLGIKMKKKAKEAIVESDSEAENYKVPEYKTAISFYSRDQLNITLSKCGLVMLNNLVEAFTEAATGSSSVFLRDLAPFMIFNSLGLTVSVSPSDSFSVLNVPLAKSYELKNDESLSMDYVRTKDNDHFNAMTSLSSKLFFILLTPANHSVADKIPLTKVGRRLYTVRHRESGVERSIICQIDTVEGSKKVTIRSPVQIKNHFSIPISVFEGDTLLGIASPENEFNIPLASYRSSLSLVPEDQDYQLCEGIDFEEIIKYDGQLLKKKCRSTNPSKKSFVINIVPEKDNLASLSVYSEDGWDLPYVLHLWPPILIRNLLPYKVAYYIEGIENTVVTLSEGHSSQIYNVEMDQAKLHLKLLDYLNHDWKSEFYIRSSQQDINFINFTCLTEMEKSDLDIAIHMTYNTGQTVVAFHSPYWMVNKTNRMLQYKADGIHRKHPPNYTKPVLFSFQPNHFFNNNKVQLMVTDSELSDQFSIDTVGSHGAIRCKGLKMEYQVGVTINLSSFNITRIVTFIPFYMIKNKSKYHISVAEEGSDKWLSLDLEQSIPFWPENASNILLIQVERSEDPPKRIYFNKQDNCILLRLNNELGGIIAEVNLAEHSTVITFSDYHDGAATFLLINHTKSDPVQYNQSSLGEIEDSLPPGKAVYYTWADPVGSRKLKWSCGQSYGEVTHKDDMMTPISVGKKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAELEVVLALQDVGISLVNNYTKQEVAYIGITSSDVVWEAKPKKKARWKPMSVKHTEKLEKEFREYTEASPLEDKVVELDNIPVRLTPSGNDMKILQPHVIPVRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAIFPFVFYPIKPPRSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLSLDLGFVYALADLVTKAEVTEKTEVEHFHKDVEAFEQEYEVVSSVDQSQVNLFEYFHISPIKLHLSVSLSSGRDEAKDSEQHGGLIPVHSLNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSELQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITSAMAKGVAAMTMDEDYQQKRREAMNKQPAGLREGITRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRATETSEVESLRPPRFFNEDGVIRPYRLRDGSGNQMLQASKSLI

Target ORF information:

RefSeq Version	XM_006527126.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527126.3

ORF Insert Sequence:

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAC GCCTGCTAAC 
CACTCTGTCG CTGACAAGAT CCCTTTAACA AAAGTGGGAC GCCGTCTGTA CACTGTAAGA 
CACAGAGAAT CTGGGGTCGA AAGATCTATC ATTTGTCAAA TTGACACAGT TGAAGGAAGT 
AAGAAGGTAA CAATCCGCTC ACCAGTGCAG ATTAAGAATC ATTTTTCAAT CCCAATTTCC 
GTTTTTGAAG GAGATACTTT ATTGGGAATT GCTTCACCTG AAAATGAATT TAACATACCA 
TTAGCATCTT ACCGATCATC CCTTTCCCTG GTGCCAGAAG ACCAGGACTA CCAGCTATGT 
GAAGGAATCG ACTTTGAAGA AATCATAAAA TATGATGGAC AGCTTCTGAA GAAGAAATGT 
AGATCTACAA ACCCTTCTAA GAAATCATTT GTTATTAATA TTGTCCCAGA AAAGGATAAT 
TTGGCTTCCC TGTCAGTGTA TTCGGAAGAT GGTTGGGACT TACCATATGT CCTGCACTTG 
TGGCCACCCA TCCTCATCCG GAACCTTCTT CCCTACAAAG TTGCTTATTA CATTGAGGGC 
ATTGAGAACA CTGTTGTTAC TCTAAGTGAA GGACATTCAT CACAGATCTA TAATGTAGAA 
ATGGACCAAG CCAAGCTACA CTTAAAATTA CTCGACTATC TTAATCATGA TTGGAAAAGT 
GAATTTTATA TAAGATCTAG TCAACAAGAC ATCAATTTCA TCAACTTTAC CTGTCTCACA 
GAAATGGAAA AGAGTGACTT GGATATTGCT ATCCATATGA CTTACAACAC TGGTCAGACA 
GTTGTGGCAT TTCATAGTCC GTATTGGATG GTCAATAAAA CCAATCGGAT GCTACAGTAC 
AAAGCAGATG GAATTCACCG AAAGCATCCA CCTAATTATA CAAAACCAGT TCTCTTTTCC 
TTTCAGCCAA ATCACTTTTT TAACAACAAT AAGGTTCAGC TTATGGTAAC TGATAGTGAG 
CTTTCAGATC AGTTTTCAAT TGATACTGTT GGTAGTCATG GAGCCATTAG ATGCAAAGGT 
CTGAAAATGG AATACCAAGT TGGTGTCACT ATAAATCTCA GCAGTTTTAA CATTACCAGA 
ATTGTGACAT TTATTCCGTT TTATATGATT AAAAACAAAA GCAAATACCA TATATCAGTA 
GCAGAAGAAG GAAGTGATAA ATGGCTTTCT CTTGACTTAG AGCAGAGTAT CCCCTTTTGG 
CCTGAGAATG CCTCCAATAT TCTTCTTATT CAAGTTGAGA GGAGTGAAGA TCCTCCAAAA 
AGGATATATT TTAACAAGCA AGATAATTGT ATTTTATTGA GACTGAATAA TGAGCTTGGA 
GGTATTATTG CAGAAGTTAA TTTGGCTGAG CACTCTACAG TTATTACATT TTCAGATTAC 
CATGATGGAG CAGCTACATT CCTATTAATA AATCATACCA AGAGTGACCC TGTTCAGTAC 
AACCAAAGTT CTCTCGGTGA AATAGAAGAT TCTCTTCCTC CTGGAAAAGC AGTCTATTAT 
ACATGGGCTG ACCCAGTGGG CTCTAGAAAG CTGAAGTGGA GCTGTGGGCA AAGCTATGGT 
GAAGTAACCC ATAAGGATGA TATGATGACG CCCATAAGTG TGGGGAAAAA AACCATTTAT 
TTAGTGTCGT TCTTTGAAGG CCTGCAGCGT ATTATTTTAT TCACTGAAGA TCCAAGAGTG 
TTTAAAGTAA CGTATGAAAG TGAAAAGGCA GAGTTAGCGG AGCTGGAAGT TGTGCTGGCG 
TTACAGGATG TCGGGATTTC ACTTGTCAAT AACTATACAA AGCAAGAAGT AGCCTACATA 
GGCATTACAA GTTCTGATGT GGTTTGGGAG GCAAAACCAA AGAAGAAGGC CAGGTGGAAG 
CCCATGAGTG TGAAGCACAC AGAGAAGCTG GAGAAGGAGT TCAGGGAGTA CACAGAGGCC 
TCGCCCTTGG AGGACAAAGT GGTCGAGTTG GACAACATTC CGGTTCGTTT AACACCTAGT 
GGCAATGACA TGAAAATTCT GCAACCACAT GTGATACCTG TCCGGAGAAA TTACCTGCCA 
GCACTAAAAG TAGAATATAA CACATCTGCA CATCAGTCGT CATTCAGAAT TCAGATTTAT 
AGAATACAGA TCCAAAATCA GATTCATGGT GCTATTTTCC CTTTCGTGTT TTATCCTATT 
AAACCTCCAA GATCTGTCAC CATGGACTCA GCACCAAAGC CGTTTACAGA TGTCAGTATT 
GTCATGAGAT CTGCAGGACA TTCCCAAATA TCACGCATTA AGTATTTCAA AGTGTTGATT 
CAAGAAATGG ACCTCAGTTT GGATCTTGGA TTTGTGTATG CCCTAGCAGA CCTTGTCACA 
AAAGCTGAAG TGACTGAGAA GACAGAGGTG GAACATTTTC ATAAGGATGT AGAAGCATTT 
GAACAAGAAT ATGAAGTTGT TTCATCAGTA GATCAATCAC AAGTCAATCT CTTTGAATAT 
TTTCATATAT CTCCTATCAA GTTGCACTTG AGTGTTTCCC TGAGCTCTGG TAGAGATGAA 
GCGAAAGACT CAGAACAACA TGGAGGACTA ATTCCTGTTC ATTCGTTAAA TCTTTTGCTG 
AAGAGTATTG GTGCCACCCT CACAGATGTA CAAGATGTAG TTTTTAAGCT TGCCTTTTTT 
GAACTCAACT ACCAGTTCCA TACAACATCT GAATTGCAAT CTGAAGTAAT AAGACACTAT 
TCCAAACAGG CTATTAAACA AATGTATGTA CTCATTCTTG GACTTGACGT CTTGGGAAAT 
CCCTTTGGCT TAATTAGAGA ATTTTCAGAA GGAGTAGAAG CGTTTTTTTA TGAACCTTAC 
CAGGGGGCTA TCCAGGGTCC TGAAGAGTTT GTGGAGGGAA TGGCGCTAGG ATTGAAAGCA 
CTAGTTGGTG GAGCCGTTGG TGGGCTAGCT GGTGCTGCCT CCAAAATCAC CAGTGCTATG 
GCTAAAGGAG TAGCGGCCAT GACCATGGAT GAAGACTACC AGCAGAAGAG GAGAGAAGCA 
ATGAATAAGC AGCCTGCTGG CCTTCGAGAG GGCATCACTC GAGGAGGAAA AGGCCTTGTC 
TCTGGTTTTG TAAGTGGCAT TACAGGGATT GTTACAAAGC CAATTAAAGG TGCTCAAAAG 
GAAGGAGCAG CAGGTTTCTT CAAAGGTGTT GGAAAAGGTT TAGTTGGAGC AGTGACAAGG 
CCAACGGGTG GCATCATAGA CATGGCCAGC AGTACCTTTC AGGGAATCAA AAGAGCTACA 
GAGACTTCTG AAGTAGAGAG TTTACGACCT CCCAGATTCT TTAATGAAGA TGGAGTCATC 
AGACCTTACA GGTTGCGCGA TGGTTCAGGA AATCAAATGT TACAGGCATC AAAAAGTTTG 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps13a ( XM_011247256.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245558.1 Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X1, mRNA.

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CloneID	OMu74479
Clone ID Related Accession (Same CDS sequence)	XM_011247256.2 , XM_011247256.3
Accession Version	XM_011247256.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 13A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAC GCCTGCTAAC 
CACTCTGTCG CTGACAAGAT CCCTTTAACA AAAGTGGGAC GCCGTCTGTA CACTGTAAGA 
CACAGAGAAT CTGGGGTCGA AAGATCTATC ATTTGTCAAA TTGACACAGT TGAAGGAAGT 
AAGAAGGTAA CAATCCGCTC ACCAGTGCAG ATTAAGAATC ATTTTTCAAT CCCAATTTCC 
GTTTTTGAAG GAGATACTTT ATTGGGAATT GCTTCACCTG AAAATGAATT TAACATACCA 
TTAGCATCTT ACCGATCATC CCTTTCCCTG GTGCCAGAAG ACCAGGACTA CCAGCTATGT 
GAAGGAATCG ACTTTGAAGA AATCATAAAA TATGATGGAC AGCTTCTGAA GAAGAAATGT 
AGATCTACAA ACCCTTCTAA GAAATCATTT GTTATTAATA TTGTCCCAGA AAAGGATAAT 
TTGGCTTCCC TGTCAGTGTA TTCGGAAGAT GGTTGGGACT TACCATATGT CCTGCACTTG 
TGGCCACCCA TCCTCATCCG GAACCTTCTT CCCTACAAAG TTGCTTATTA CATTGAGGGC 
ATTGAGAACA CTGTTGTTAC TCTAAGTGAA GGACATTCAT CACAGATCTA TAATGTAGAA 
ATGGACCAAG CCAAGCTACA CTTAAAATTA CTCGACTATC TTAATCATGA TTGGAAAAGT 
GAATTTTATA TAAGATCTAG TCAACAAGAC ATCAATTTCA TCAACTTTAC CTGTCTCACA 
GAAATGGAAA AGAGTGACTT GGATATTGCT ATCCATATGA CTTACAACAC TGGTCAGACA 
GTTGTGGCAT TTCATAGTCC GTATTGGATG GTCAATAAAA CCAATCGGAT GCTACAGTAC 
AAAGCAGATG GAATTCACCG AAAGCATCCA CCTAATTATA CAAAACCAGT TCTCTTTTCC 
TTTCAGCCAA ATCACTTTTT TAACAACAAT AAGGTTCAGC TTATGGTAAC TGATAGTGAG 
CTTTCAGATC AGTTTTCAAT TGATACTGTT GGTAGTCATG GAGCCATTAG ATGCAAAGGT 
CTGAAAATGG AATACCAAGT TGGTGTCACT ATAAATCTCA GCAGTTTTAA CATTACCAGA 
ATTGTGACAT TTATTCCGTT TTATATGATT AAAAACAAAA GCAAATACCA TATATCAGTA 
GCAGAAGAAG GAAGTGATAA ATGGCTTTCT CTTGACTTAG AGCAGAGTAT CCCCTTTTGG 
CCTGAGAATG CCTCCAATAT TCTTCTTATT CAAGTTGAGA GGAGTGAAGA TCCTCCAAAA 
AGGATATATT TTAACAAGCA AGATAATTGT ATTTTATTGA GACTGAATAA TGAGCTTGGA 
GGTATTATTG CAGAAGTTAA TTTGGCTGAG CACTCTACAG TTATTACATT TTCAGATTAC 
CATGATGGAG CAGCTACATT CCTATTAATA AATCATACCA AGAGTGACCC TGTTCAGTAC 
AACCAAAGTT CTCTCGGTGA AATAGAAGAT TCTCTTCCTC CTGGAAAAGC AGTCTATTAT 
ACATGGGCTG ACCCAGTGGG CTCTAGAAAG CTGAAGTGGA GCTGTGGGCA AAGCTATGGT 
GAAGTAACCC ATAAGGATGA TATGATGACG CCCATAAGTG TGGGGAAAAA AACCATTTAT 
TTAGTGTCGT TCTTTGAAGG CCTGCAGCGT ATTATTTTAT TCACTGAAGA TCCAAGAGTG 
TTTAAAGTAA CGTATGAAAG TGAAAAGGCA GAGTTAGCGG AGCTGGAAGT TGTGCTGGCG 
TTACAGGATG TCGGGATTTC ACTTGTCAAT AACTATACAA AGCAAGAAGT AGCCTACATA 
GGCATTACAA GTTCTGATGT GGTTTGGGAG GCAAAACCAA AGAAGAAGGC CAGGTGGAAG 
CCCATGAGTG TGAAGCACAC AGAGAAGCTG GAGAAGGAGT TCAGGGAGTA CACAGAGGCC 
TCGCCCTTGG AGGACAAAGT GGTCGAGTTG GACAACATTC CGGTTCGTTT AACACCTAGT 
GGCAATGACA TGAAAATTCT GCAACCACAT GTGATACCTG TCCGGAGAAA TTACCTGCCA 
GCACTAAAAG TAGAATATAA CACATCTGCA CATCAGTCGT CATTCAGAAT TCAGATTTAT 
AGAATACAGA TCCAAAATCA GATTCATGGT GCTATTTTCC CTTTCGTGTT TTATCCTATT 
AAACCTCCAA GATCTGTCAC CATGGACTCA GCACCAAAGC CGTTTACAGA TGTCAGTATT 
GTCATGAGAT CTGCAGGACA TTCCCAAATA TCACGCATTA AGTATTTCAA AGTGTTGATT 
CAAGAAATGG ACCTCAGTTT GGATCTTGGA TTTGTGTATG CCCTAGCAGA CCTTGTCACA 
AAAGCTGAAG TGACTGAGAA GACAGAGGTG GAACATTTTC ATAAGGATGT AGAAGCATTT 
GAACAAGAAT ATGAAGTTGT TTCATCAGTA GATCAATCAC AAGTCAATCT CTTTGAATAT 
TTTCATATAT CTCCTATCAA GTTGCACTTG AGTGTTTCCC TGAGCTCTGG TAGAGATGAA 
GCGAAAGACT CAGAACAACA TGGAGGACTA ATTCCTGTTC ATTCGTTAAA TCTTTTGCTG 
AAGAGTATTG GTGCCACCCT CACAGATGTA CAAGATGTAG TTTTTAAGCT TGCCTTTTTT 
GAACTCAACT ACCAGTTCCA TACAACATCT GAATTGCAAT CTGAAGTAAT AAGACACTAT 
TCCAAACAGG CTATTAAACA AATGTATGTA CTCATTCTTG GACTTGACGT CTTGGGAAAT 
CCCTTTGGCT TAATTAGAGA ATTTTCAGAA GGAGTAGAAG CGTTTTTTTA TGAACCTTAC 
CAGGGGGCTA TCCAGGGTCC TGAAGAGTTT GTGGAGGGAA TGGCGCTAGG ATTGAAAGCA 
CTAGTTGGTG GAGCCGTTGG TGGGCTAGCT GGTGCTGCCT CCAAAATCAC CAGTGCTATG 
GCTAAAGGAG TAGCGGCCAT GACCATGGAT GAAGACTACC AGCAGAAGAG GAGAGAAGCA 
ATGAATAAGC AGCCTGCTGG CCTTCGAGAG GGCATCACTC GAGGAGGAAA AGGCCTTGTC 
TCTGGTTTTG TAAGTGGCAT TACAGGGATT GTTACAAAGC CAATTAAAGG TGCTCAAAAG 
GAAGGAGCAG CAGGTTTCTT CAAAGGTGTT GGAAAAGGTT TAGTTGGAGC AGTGACAAGG 
CCAACGGGTG GCATCATAGA CATGGCCAGC AGTACCTTTC AGGGAATCAA AAGAGCTACA 
GAGACTTCTG AAGTAGAGAG TTTACGACCT CCCAGATTCT TTAATGAAGA TGGAGTCATC 
AGACCTTACA GGTTGCGCGA TGGTTCAGGA AATCAAATGT TACAGAACAT TCAGGCCTAC 
AGGGAATGGA TTCTGACTCA CAGTAGCAGT AGTGAGGAGG AGGAGGAGGA GGAGGAAGAG 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245558.1
CDS	325..9570
Translation	MVFESVVVEVLNRFLGDYVVNLDESQLSLGIWKGAVALKNLVIKENALHELDVPFKVKVGHIGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCLHDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKALKNGIVNENIVPEGYDFVFRPISASAKLQMNRRSDFDFSDPKINLAVDLHTIAIEFNKPQYFSLMELLESIDMMTQNQPYRKFKPSVPLHLHAKEWWAYAIHSILEVNVCPSLRMWSWEHIRNHRYKMKRYREFYKKKLTSKKPSPEILMSLEELEKTLDVFNITIARQQAEVEAKKAGYKIYKEGVKDPEDNAGWFGWLWTWSESNANQQQDVKPGILEEMLTPEEKSLLYEAIGYSETAVDPTLPKTFEALKFFVHLKSMSIVLRENHQKPELLNVVVEGLSTSVVQRPGAQAIKFETKIDSFHITGLPDDFKKPHLLSSLDDTSLLQITFEINPLNETVAQRCTIEAEPLEIIYDARTVNSIVEFFRPPKDVHLAQLTSVTLTKLEEFRAKTATGLLYVIETQKVLDLRINVKASYVIVPQYGNFSPTSNLLLLDLGHLKVSSKRRSLLPDVRPSEASLEDIMHRAYDSFDIQLTSIQLLYSRVGDNWKEARKLNVSTQHILIPMHVNVELSKAMVFMDIKMPKFKISGKLPLVSLRISDKKLQGIMELLGSIPKPEPVTDVSAPARSFQIQASALPVSHISQKLIPLLEQPVTEDDSEEEFFDAPCSPLEECPQVSCRDKCTRQKKLQKKDCVMNLIQLRMRFEVAEVSIQFYHLVGDCELPVLEMGALGLGTEAEFRTFDLKGSAFLKELWLKCPEYLDENKKPVYLITTLDNTMEDLLTLEFMKVEKNAPNLNSTYNNVLQLIKVNFSSLDIHLHTEALLNTMNYLNNILPELREKSASVSAAEPEDKGDIIKKLALKLPTNEDIITLQLLAELSCLQIFIQDQKQNISEIKIEGLDSEMIMKPLVTEINAKLRNIIVLDSDKMAIYKKALYITGKEVFSFKMISYMDATAGYAYTDMSVVDIRVHLTVGCIEVVFITKFLYSILAFIDNFQAVKDALAEATVQAAEMAADGVKELARKSSRFALDVNIKAPVVLIPQSPVSQNVFVADFGLITMKNIFVTVTETQSNIPPVIDLITIKLSKMRLYRSQFRNDTYQEVLDLLLPLNLEVIVERNLSWEWYKEVPCFNIKAQLKPMEFILSQEDLTTVFQTLHGNIWYGQDLSAPSSANKDPETMTSGVTSPPDHSPATVVTAAVVEVHPQASQAHTMLNVSFQTDYLTMALYSPGPDEASFTDVRDPSLELAEFKLENIISSLKIYTDDSTVFSFSVKNCILDDKRSHVMKATPRMIGLTVGFDKKDMVDIKYRKIKTFVVTDAVVQEMYVCASVEFLMTVAHIFFDAYMTSTALETSVQTRTTREAPAQELGKWEMNILIKNPEIVFVADMTRNDAPALVITTQCEICCKGEPTSNTVTAAIKDLQVRACPFLPVKRKGKVTTVLQPCDLFYQATQLGRDPQMIDISVKSLTLKVSPVIINTIITITSALYTTKETVPEENTSNIAHLWDKKDTKNLKMWFLEESNESEKVVPTNEVMPGGETLNLRIDSIFIVLEAGIGHRTVPMLLAKACFSGESKNWLSLINLHCHLELEVHYYNEMFGVWEPLLEPLEIDQTDDFRPWNLGIKMKKKAKEAIVESDSEAENYKVPEYKTAISFYSRDQLNITLSKCGLVMLNNLVEAFTEAATGSSSVFLRDLAPFMIFNSLGLTVSVSPSDSFSVLNVPLAKSYELKNDESLSMDYVRTKDNDHFNAMTSLSSKLFFILLTPANHSVADKIPLTKVGRRLYTVRHRESGVERSIICQIDTVEGSKKVTIRSPVQIKNHFSIPISVFEGDTLLGIASPENEFNIPLASYRSSLSLVPEDQDYQLCEGIDFEEIIKYDGQLLKKKCRSTNPSKKSFVINIVPEKDNLASLSVYSEDGWDLPYVLHLWPPILIRNLLPYKVAYYIEGIENTVVTLSEGHSSQIYNVEMDQAKLHLKLLDYLNHDWKSEFYIRSSQQDINFINFTCLTEMEKSDLDIAIHMTYNTGQTVVAFHSPYWMVNKTNRMLQYKADGIHRKHPPNYTKPVLFSFQPNHFFNNNKVQLMVTDSELSDQFSIDTVGSHGAIRCKGLKMEYQVGVTINLSSFNITRIVTFIPFYMIKNKSKYHISVAEEGSDKWLSLDLEQSIPFWPENASNILLIQVERSEDPPKRIYFNKQDNCILLRLNNELGGIIAEVNLAEHSTVITFSDYHDGAATFLLINHTKSDPVQYNQSSLGEIEDSLPPGKAVYYTWADPVGSRKLKWSCGQSYGEVTHKDDMMTPISVGKKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAELEVVLALQDVGISLVNNYTKQEVAYIGITSSDVVWEAKPKKKARWKPMSVKHTEKLEKEFREYTEASPLEDKVVELDNIPVRLTPSGNDMKILQPHVIPVRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAIFPFVFYPIKPPRSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLSLDLGFVYALADLVTKAEVTEKTEVEHFHKDVEAFEQEYEVVSSVDQSQVNLFEYFHISPIKLHLSVSLSSGRDEAKDSEQHGGLIPVHSLNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSELQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITSAMAKGVAAMTMDEDYQQKRREAMNKQPAGLREGITRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRATETSEVESLRPPRFFNEDGVIRPYRLRDGSGNQMLQNIQAYREWILTHSSSSEEEEEEEEET

Target ORF information:

RefSeq Version	XM_011247256.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247256.2

ORF Insert Sequence:

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAC GCCTGCTAAC 
CACTCTGTCG CTGACAAGAT CCCTTTAACA AAAGTGGGAC GCCGTCTGTA CACTGTAAGA 
CACAGAGAAT CTGGGGTCGA AAGATCTATC ATTTGTCAAA TTGACACAGT TGAAGGAAGT 
AAGAAGGTAA CAATCCGCTC ACCAGTGCAG ATTAAGAATC ATTTTTCAAT CCCAATTTCC 
GTTTTTGAAG GAGATACTTT ATTGGGAATT GCTTCACCTG AAAATGAATT TAACATACCA 
TTAGCATCTT ACCGATCATC CCTTTCCCTG GTGCCAGAAG ACCAGGACTA CCAGCTATGT 
GAAGGAATCG ACTTTGAAGA AATCATAAAA TATGATGGAC AGCTTCTGAA GAAGAAATGT 
AGATCTACAA ACCCTTCTAA GAAATCATTT GTTATTAATA TTGTCCCAGA AAAGGATAAT 
TTGGCTTCCC TGTCAGTGTA TTCGGAAGAT GGTTGGGACT TACCATATGT CCTGCACTTG 
TGGCCACCCA TCCTCATCCG GAACCTTCTT CCCTACAAAG TTGCTTATTA CATTGAGGGC 
ATTGAGAACA CTGTTGTTAC TCTAAGTGAA GGACATTCAT CACAGATCTA TAATGTAGAA 
ATGGACCAAG CCAAGCTACA CTTAAAATTA CTCGACTATC TTAATCATGA TTGGAAAAGT 
GAATTTTATA TAAGATCTAG TCAACAAGAC ATCAATTTCA TCAACTTTAC CTGTCTCACA 
GAAATGGAAA AGAGTGACTT GGATATTGCT ATCCATATGA CTTACAACAC TGGTCAGACA 
GTTGTGGCAT TTCATAGTCC GTATTGGATG GTCAATAAAA CCAATCGGAT GCTACAGTAC 
AAAGCAGATG GAATTCACCG AAAGCATCCA CCTAATTATA CAAAACCAGT TCTCTTTTCC 
TTTCAGCCAA ATCACTTTTT TAACAACAAT AAGGTTCAGC TTATGGTAAC TGATAGTGAG 
CTTTCAGATC AGTTTTCAAT TGATACTGTT GGTAGTCATG GAGCCATTAG ATGCAAAGGT 
CTGAAAATGG AATACCAAGT TGGTGTCACT ATAAATCTCA GCAGTTTTAA CATTACCAGA 
ATTGTGACAT TTATTCCGTT TTATATGATT AAAAACAAAA GCAAATACCA TATATCAGTA 
GCAGAAGAAG GAAGTGATAA ATGGCTTTCT CTTGACTTAG AGCAGAGTAT CCCCTTTTGG 
CCTGAGAATG CCTCCAATAT TCTTCTTATT CAAGTTGAGA GGAGTGAAGA TCCTCCAAAA 
AGGATATATT TTAACAAGCA AGATAATTGT ATTTTATTGA GACTGAATAA TGAGCTTGGA 
GGTATTATTG CAGAAGTTAA TTTGGCTGAG CACTCTACAG TTATTACATT TTCAGATTAC 
CATGATGGAG CAGCTACATT CCTATTAATA AATCATACCA AGAGTGACCC TGTTCAGTAC 
AACCAAAGTT CTCTCGGTGA AATAGAAGAT TCTCTTCCTC CTGGAAAAGC AGTCTATTAT 
ACATGGGCTG ACCCAGTGGG CTCTAGAAAG CTGAAGTGGA GCTGTGGGCA AAGCTATGGT 
GAAGTAACCC ATAAGGATGA TATGATGACG CCCATAAGTG TGGGGAAAAA AACCATTTAT 
TTAGTGTCGT TCTTTGAAGG CCTGCAGCGT ATTATTTTAT TCACTGAAGA TCCAAGAGTG 
TTTAAAGTAA CGTATGAAAG TGAAAAGGCA GAGTTAGCGG AGCTGGAAGT TGTGCTGGCG 
TTACAGGATG TCGGGATTTC ACTTGTCAAT AACTATACAA AGCAAGAAGT AGCCTACATA 
GGCATTACAA GTTCTGATGT GGTTTGGGAG GCAAAACCAA AGAAGAAGGC CAGGTGGAAG 
CCCATGAGTG TGAAGCACAC AGAGAAGCTG GAGAAGGAGT TCAGGGAGTA CACAGAGGCC 
TCGCCCTTGG AGGACAAAGT GGTCGAGTTG GACAACATTC CGGTTCGTTT AACACCTAGT 
GGCAATGACA TGAAAATTCT GCAACCACAT GTGATACCTG TCCGGAGAAA TTACCTGCCA 
GCACTAAAAG TAGAATATAA CACATCTGCA CATCAGTCGT CATTCAGAAT TCAGATTTAT 
AGAATACAGA TCCAAAATCA GATTCATGGT GCTATTTTCC CTTTCGTGTT TTATCCTATT 
AAACCTCCAA GATCTGTCAC CATGGACTCA GCACCAAAGC CGTTTACAGA TGTCAGTATT 
GTCATGAGAT CTGCAGGACA TTCCCAAATA TCACGCATTA AGTATTTCAA AGTGTTGATT 
CAAGAAATGG ACCTCAGTTT GGATCTTGGA TTTGTGTATG CCCTAGCAGA CCTTGTCACA 
AAAGCTGAAG TGACTGAGAA GACAGAGGTG GAACATTTTC ATAAGGATGT AGAAGCATTT 
GAACAAGAAT ATGAAGTTGT TTCATCAGTA GATCAATCAC AAGTCAATCT CTTTGAATAT 
TTTCATATAT CTCCTATCAA GTTGCACTTG AGTGTTTCCC TGAGCTCTGG TAGAGATGAA 
GCGAAAGACT CAGAACAACA TGGAGGACTA ATTCCTGTTC ATTCGTTAAA TCTTTTGCTG 
AAGAGTATTG GTGCCACCCT CACAGATGTA CAAGATGTAG TTTTTAAGCT TGCCTTTTTT 
GAACTCAACT ACCAGTTCCA TACAACATCT GAATTGCAAT CTGAAGTAAT AAGACACTAT 
TCCAAACAGG CTATTAAACA AATGTATGTA CTCATTCTTG GACTTGACGT CTTGGGAAAT 
CCCTTTGGCT TAATTAGAGA ATTTTCAGAA GGAGTAGAAG CGTTTTTTTA TGAACCTTAC 
CAGGGGGCTA TCCAGGGTCC TGAAGAGTTT GTGGAGGGAA TGGCGCTAGG ATTGAAAGCA 
CTAGTTGGTG GAGCCGTTGG TGGGCTAGCT GGTGCTGCCT CCAAAATCAC CAGTGCTATG 
GCTAAAGGAG TAGCGGCCAT GACCATGGAT GAAGACTACC AGCAGAAGAG GAGAGAAGCA 
ATGAATAAGC AGCCTGCTGG CCTTCGAGAG GGCATCACTC GAGGAGGAAA AGGCCTTGTC 
TCTGGTTTTG TAAGTGGCAT TACAGGGATT GTTACAAAGC CAATTAAAGG TGCTCAAAAG 
GAAGGAGCAG CAGGTTTCTT CAAAGGTGTT GGAAAAGGTT TAGTTGGAGC AGTGACAAGG 
CCAACGGGTG GCATCATAGA CATGGCCAGC AGTACCTTTC AGGGAATCAA AAGAGCTACA 
GAGACTTCTG AAGTAGAGAG TTTACGACCT CCCAGATTCT TTAATGAAGA TGGAGTCATC 
AGACCTTACA GGTTGCGCGA TGGTTCAGGA AATCAAATGT TACAGAACAT TCAGGCCTAC 
AGGGAATGGA TTCTGACTCA CAGTAGCAGT AGTGAGGAGG AGGAGGAGGA GGAGGAAGAG 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps13a ( XM_011247256.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245558.1 Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X1, mRNA.

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CloneID	OMu74479
Clone ID Related Accession (Same CDS sequence)	XM_011247256.2 , XM_011247256.3
Accession Version	XM_011247256.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 13A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAC GCCTGCTAAC 
CACTCTGTCG CTGACAAGAT CCCTTTAACA AAAGTGGGAC GCCGTCTGTA CACTGTAAGA 
CACAGAGAAT CTGGGGTCGA AAGATCTATC ATTTGTCAAA TTGACACAGT TGAAGGAAGT 
AAGAAGGTAA CAATCCGCTC ACCAGTGCAG ATTAAGAATC ATTTTTCAAT CCCAATTTCC 
GTTTTTGAAG GAGATACTTT ATTGGGAATT GCTTCACCTG AAAATGAATT TAACATACCA 
TTAGCATCTT ACCGATCATC CCTTTCCCTG GTGCCAGAAG ACCAGGACTA CCAGCTATGT 
GAAGGAATCG ACTTTGAAGA AATCATAAAA TATGATGGAC AGCTTCTGAA GAAGAAATGT 
AGATCTACAA ACCCTTCTAA GAAATCATTT GTTATTAATA TTGTCCCAGA AAAGGATAAT 
TTGGCTTCCC TGTCAGTGTA TTCGGAAGAT GGTTGGGACT TACCATATGT CCTGCACTTG 
TGGCCACCCA TCCTCATCCG GAACCTTCTT CCCTACAAAG TTGCTTATTA CATTGAGGGC 
ATTGAGAACA CTGTTGTTAC TCTAAGTGAA GGACATTCAT CACAGATCTA TAATGTAGAA 
ATGGACCAAG CCAAGCTACA CTTAAAATTA CTCGACTATC TTAATCATGA TTGGAAAAGT 
GAATTTTATA TAAGATCTAG TCAACAAGAC ATCAATTTCA TCAACTTTAC CTGTCTCACA 
GAAATGGAAA AGAGTGACTT GGATATTGCT ATCCATATGA CTTACAACAC TGGTCAGACA 
GTTGTGGCAT TTCATAGTCC GTATTGGATG GTCAATAAAA CCAATCGGAT GCTACAGTAC 
AAAGCAGATG GAATTCACCG AAAGCATCCA CCTAATTATA CAAAACCAGT TCTCTTTTCC 
TTTCAGCCAA ATCACTTTTT TAACAACAAT AAGGTTCAGC TTATGGTAAC TGATAGTGAG 
CTTTCAGATC AGTTTTCAAT TGATACTGTT GGTAGTCATG GAGCCATTAG ATGCAAAGGT 
CTGAAAATGG AATACCAAGT TGGTGTCACT ATAAATCTCA GCAGTTTTAA CATTACCAGA 
ATTGTGACAT TTATTCCGTT TTATATGATT AAAAACAAAA GCAAATACCA TATATCAGTA 
GCAGAAGAAG GAAGTGATAA ATGGCTTTCT CTTGACTTAG AGCAGAGTAT CCCCTTTTGG 
CCTGAGAATG CCTCCAATAT TCTTCTTATT CAAGTTGAGA GGAGTGAAGA TCCTCCAAAA 
AGGATATATT TTAACAAGCA AGATAATTGT ATTTTATTGA GACTGAATAA TGAGCTTGGA 
GGTATTATTG CAGAAGTTAA TTTGGCTGAG CACTCTACAG TTATTACATT TTCAGATTAC 
CATGATGGAG CAGCTACATT CCTATTAATA AATCATACCA AGAGTGACCC TGTTCAGTAC 
AACCAAAGTT CTCTCGGTGA AATAGAAGAT TCTCTTCCTC CTGGAAAAGC AGTCTATTAT 
ACATGGGCTG ACCCAGTGGG CTCTAGAAAG CTGAAGTGGA GCTGTGGGCA AAGCTATGGT 
GAAGTAACCC ATAAGGATGA TATGATGACG CCCATAAGTG TGGGGAAAAA AACCATTTAT 
TTAGTGTCGT TCTTTGAAGG CCTGCAGCGT ATTATTTTAT TCACTGAAGA TCCAAGAGTG 
TTTAAAGTAA CGTATGAAAG TGAAAAGGCA GAGTTAGCGG AGCTGGAAGT TGTGCTGGCG 
TTACAGGATG TCGGGATTTC ACTTGTCAAT AACTATACAA AGCAAGAAGT AGCCTACATA 
GGCATTACAA GTTCTGATGT GGTTTGGGAG GCAAAACCAA AGAAGAAGGC CAGGTGGAAG 
CCCATGAGTG TGAAGCACAC AGAGAAGCTG GAGAAGGAGT TCAGGGAGTA CACAGAGGCC 
TCGCCCTTGG AGGACAAAGT GGTCGAGTTG GACAACATTC CGGTTCGTTT AACACCTAGT 
GGCAATGACA TGAAAATTCT GCAACCACAT GTGATACCTG TCCGGAGAAA TTACCTGCCA 
GCACTAAAAG TAGAATATAA CACATCTGCA CATCAGTCGT CATTCAGAAT TCAGATTTAT 
AGAATACAGA TCCAAAATCA GATTCATGGT GCTATTTTCC CTTTCGTGTT TTATCCTATT 
AAACCTCCAA GATCTGTCAC CATGGACTCA GCACCAAAGC CGTTTACAGA TGTCAGTATT 
GTCATGAGAT CTGCAGGACA TTCCCAAATA TCACGCATTA AGTATTTCAA AGTGTTGATT 
CAAGAAATGG ACCTCAGTTT GGATCTTGGA TTTGTGTATG CCCTAGCAGA CCTTGTCACA 
AAAGCTGAAG TGACTGAGAA GACAGAGGTG GAACATTTTC ATAAGGATGT AGAAGCATTT 
GAACAAGAAT ATGAAGTTGT TTCATCAGTA GATCAATCAC AAGTCAATCT CTTTGAATAT 
TTTCATATAT CTCCTATCAA GTTGCACTTG AGTGTTTCCC TGAGCTCTGG TAGAGATGAA 
GCGAAAGACT CAGAACAACA TGGAGGACTA ATTCCTGTTC ATTCGTTAAA TCTTTTGCTG 
AAGAGTATTG GTGCCACCCT CACAGATGTA CAAGATGTAG TTTTTAAGCT TGCCTTTTTT 
GAACTCAACT ACCAGTTCCA TACAACATCT GAATTGCAAT CTGAAGTAAT AAGACACTAT 
TCCAAACAGG CTATTAAACA AATGTATGTA CTCATTCTTG GACTTGACGT CTTGGGAAAT 
CCCTTTGGCT TAATTAGAGA ATTTTCAGAA GGAGTAGAAG CGTTTTTTTA TGAACCTTAC 
CAGGGGGCTA TCCAGGGTCC TGAAGAGTTT GTGGAGGGAA TGGCGCTAGG ATTGAAAGCA 
CTAGTTGGTG GAGCCGTTGG TGGGCTAGCT GGTGCTGCCT CCAAAATCAC CAGTGCTATG 
GCTAAAGGAG TAGCGGCCAT GACCATGGAT GAAGACTACC AGCAGAAGAG GAGAGAAGCA 
ATGAATAAGC AGCCTGCTGG CCTTCGAGAG GGCATCACTC GAGGAGGAAA AGGCCTTGTC 
TCTGGTTTTG TAAGTGGCAT TACAGGGATT GTTACAAAGC CAATTAAAGG TGCTCAAAAG 
GAAGGAGCAG CAGGTTTCTT CAAAGGTGTT GGAAAAGGTT TAGTTGGAGC AGTGACAAGG 
CCAACGGGTG GCATCATAGA CATGGCCAGC AGTACCTTTC AGGGAATCAA AAGAGCTACA 
GAGACTTCTG AAGTAGAGAG TTTACGACCT CCCAGATTCT TTAATGAAGA TGGAGTCATC 
AGACCTTACA GGTTGCGCGA TGGTTCAGGA AATCAAATGT TACAGAACAT TCAGGCCTAC 
AGGGAATGGA TTCTGACTCA CAGTAGCAGT AGTGAGGAGG AGGAGGAGGA GGAGGAAGAG 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245558.1
CDS	301..9546
Translation	MVFESVVVEVLNRFLGDYVVNLDESQLSLGIWKGAVALKNLVIKENALHELDVPFKVKVGHIGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCLHDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKALKNGIVNENIVPEGYDFVFRPISASAKLQMNRRSDFDFSDPKINLAVDLHTIAIEFNKPQYFSLMELLESIDMMTQNQPYRKFKPSVPLHLHAKEWWAYAIHSILEVNVCPSLRMWSWEHIRNHRYKMKRYREFYKKKLTSKKPSPEILMSLEELEKTLDVFNITIARQQAEVEAKKAGYKIYKEGVKDPEDNAGWFGWLWTWSESNANQQQDVKPGILEEMLTPEEKSLLYEAIGYSETAVDPTLPKTFEALKFFVHLKSMSIVLRENHQKPELLNVVVEGLSTSVVQRPGAQAIKFETKIDSFHITGLPDDFKKPHLLSSLDDTSLLQITFEINPLNETVAQRCTIEAEPLEIIYDARTVNSIVEFFRPPKDVHLAQLTSVTLTKLEEFRAKTATGLLYVIETQKVLDLRINVKASYVIVPQYGNFSPTSNLLLLDLGHLKVSSKRRSLLPDVRPSEASLEDIMHRAYDSFDIQLTSIQLLYSRVGDNWKEARKLNVSTQHILIPMHVNVELSKAMVFMDIKMPKFKISGKLPLVSLRISDKKLQGIMELLGSIPKPEPVTDVSAPARSFQIQASALPVSHISQKLIPLLEQPVTEDDSEEEFFDAPCSPLEECPQVSCRDKCTRQKKLQKKDCVMNLIQLRMRFEVAEVSIQFYHLVGDCELPVLEMGALGLGTEAEFRTFDLKGSAFLKELWLKCPEYLDENKKPVYLITTLDNTMEDLLTLEFMKVEKNAPNLNSTYNNVLQLIKVNFSSLDIHLHTEALLNTMNYLNNILPELREKSASVSAAEPEDKGDIIKKLALKLPTNEDIITLQLLAELSCLQIFIQDQKQNISEIKIEGLDSEMIMKPLVTEINAKLRNIIVLDSDKMAIYKKALYITGKEVFSFKMISYMDATAGYAYTDMSVVDIRVHLTVGCIEVVFITKFLYSILAFIDNFQAVKDALAEATVQAAEMAADGVKELARKSSRFALDVNIKAPVVLIPQSPVSQNVFVADFGLITMKNIFVTVTETQSNIPPVIDLITIKLSKMRLYRSQFRNDTYQEVLDLLLPLNLEVIVERNLSWEWYKEVPCFNIKAQLKPMEFILSQEDLTTVFQTLHGNIWYGQDLSAPSSANKDPETMTSGVTSPPDHSPATVVTAAVVEVHPQASQAHTMLNVSFQTDYLTMALYSPGPDEASFTDVRDPSLELAEFKLENIISSLKIYTDDSTVFSFSVKNCILDDKRSHVMKATPRMIGLTVGFDKKDMVDIKYRKIKTFVVTDAVVQEMYVCASVEFLMTVAHIFFDAYMTSTALETSVQTRTTREAPAQELGKWEMNILIKNPEIVFVADMTRNDAPALVITTQCEICCKGEPTSNTVTAAIKDLQVRACPFLPVKRKGKVTTVLQPCDLFYQATQLGRDPQMIDISVKSLTLKVSPVIINTIITITSALYTTKETVPEENTSNIAHLWDKKDTKNLKMWFLEESNESEKVVPTNEVMPGGETLNLRIDSIFIVLEAGIGHRTVPMLLAKACFSGESKNWLSLINLHCHLELEVHYYNEMFGVWEPLLEPLEIDQTDDFRPWNLGIKMKKKAKEAIVESDSEAENYKVPEYKTAISFYSRDQLNITLSKCGLVMLNNLVEAFTEAATGSSSVFLRDLAPFMIFNSLGLTVSVSPSDSFSVLNVPLAKSYELKNDESLSMDYVRTKDNDHFNAMTSLSSKLFFILLTPANHSVADKIPLTKVGRRLYTVRHRESGVERSIICQIDTVEGSKKVTIRSPVQIKNHFSIPISVFEGDTLLGIASPENEFNIPLASYRSSLSLVPEDQDYQLCEGIDFEEIIKYDGQLLKKKCRSTNPSKKSFVINIVPEKDNLASLSVYSEDGWDLPYVLHLWPPILIRNLLPYKVAYYIEGIENTVVTLSEGHSSQIYNVEMDQAKLHLKLLDYLNHDWKSEFYIRSSQQDINFINFTCLTEMEKSDLDIAIHMTYNTGQTVVAFHSPYWMVNKTNRMLQYKADGIHRKHPPNYTKPVLFSFQPNHFFNNNKVQLMVTDSELSDQFSIDTVGSHGAIRCKGLKMEYQVGVTINLSSFNITRIVTFIPFYMIKNKSKYHISVAEEGSDKWLSLDLEQSIPFWPENASNILLIQVERSEDPPKRIYFNKQDNCILLRLNNELGGIIAEVNLAEHSTVITFSDYHDGAATFLLINHTKSDPVQYNQSSLGEIEDSLPPGKAVYYTWADPVGSRKLKWSCGQSYGEVTHKDDMMTPISVGKKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAELEVVLALQDVGISLVNNYTKQEVAYIGITSSDVVWEAKPKKKARWKPMSVKHTEKLEKEFREYTEASPLEDKVVELDNIPVRLTPSGNDMKILQPHVIPVRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAIFPFVFYPIKPPRSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLSLDLGFVYALADLVTKAEVTEKTEVEHFHKDVEAFEQEYEVVSSVDQSQVNLFEYFHISPIKLHLSVSLSSGRDEAKDSEQHGGLIPVHSLNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSELQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITSAMAKGVAAMTMDEDYQQKRREAMNKQPAGLREGITRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRATETSEVESLRPPRFFNEDGVIRPYRLRDGSGNQMLQNIQAYREWILTHSSSSEEEEEEEEET

Target ORF information:

RefSeq Version	XM_011247256.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus vacuolar protein sorting 13A (Vps13a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247256.3

ORF Insert Sequence:

ATGGTATTCG AGTCGGTGGT CGTGGAAGTG TTGAACCGGT TCCTGGGAGA CTATGTGGTG 
AACCTGGACG AGTCCCAGCT CTCCCTGGGC ATTTGGAAAG GAGCTGTGGC CCTCAAGAAC 
CTTGTAATTA AAGAAAATGC ATTGCATGAA TTGGATGTAC CATTCAAAGT TAAAGTTGGT 
CATATAGGTA GTCTTAAACT TAAAATTCCA TGGAAAAACC TTTATACTCA ACCAGTTGAA 
GCTGTTTTGG AAGAAATTTT TTTACTCATA GTGCCTTCTT CTAGAATACA GTATGATCCT 
ATCAAAGAAG AAAAGCAACT CATGGAAACA AAGCAACAGG AACTAAAAAG AATAGAAAAA 
GCAAAACAGA AAGTATTTGA TAAAGAAAAG CCTCGGGAGG AGAAACAGGA CACGTTTACA 
GAAAAGTTAG TTACTCAGAT CATACAGAAC CTTCAAGTGC AAATCTCCAG CATCCATATT 
CGTTATGAAG ACGATATCAC AAATGGAGAC AAACCACTCT CTTTTGGTAT TTCCCTTCAA 
AATATCAGCC TTCAGACAAC TGATCAATAC TGGATCCCAT GTTTACATGA TAATACTGAA 
AAGCTAGTCC GGAAGCTAAT CCGATTAGAT AATCTCTTTG CTTACTGGAA TGTGAACTCT 
GAGATGTTTT ATCTGAATGA TTACGATGAA TCGCTGAAAG CCTTGAAAAA TGGTATTGTG 
AATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTTTTTC GACCTATATC TGCTAGTGCC 
AAACTTCAGA TGAATCGACG ATCAGATTTT GACTTTTCTG ACCCCAAAAT AAACCTGGCT 
GTTGATTTAC ATACCATAGC AATTGAGTTT AATAAACCAC AGTATTTCAG CCTCATGGAG 
CTTCTCGAGT CAATTGACAT GATGACACAA AACCAGCCGT ACAGAAAGTT CAAGCCCAGT 
GTGCCTCTCC ACCTCCACGC CAAAGAGTGG TGGGCTTATG CTATACATAG TATTCTTGAA 
GTAAATGTTT GTCCCAGTTT ACGGATGTGG TCGTGGGAGC ATATTAGAAA CCATAGATAC 
AAAATGAAAC GATACAGAGA ATTTTATAAG AAAAAATTAA CAAGTAAGAA GCCATCTCCT 
GAAATTCTCA TGTCTTTGGA GGAGTTGGAG AAAACTTTGG ATGTTTTTAA TATAACCATA 
GCTAGACAAC AGGCAGAAGT TGAGGCAAAG AAAGCTGGAT ATAAAATTTA CAAAGAAGGA 
GTCAAAGATC CAGAAGATAA TGCAGGATGG TTTGGCTGGT TGTGGACTTG GTCAGAATCA 
AATGCTAATC AACAACAAGA TGTGAAGCCT GGAATTCTGG AAGAAATGTT GACACCTGAA 
GAAAAGTCGT TACTTTATGA AGCAATTGGT TATAGTGAAA CAGCAGTTGA CCCAACCTTG 
CCAAAAACAT TTGAAGCCTT GAAGTTTTTT GTTCACTTGA AAAGCATGTC TATTGTTCTA 
AGAGAAAATC ACCAAAAGCC GGAGCTGTTA AACGTTGTAG TGGAAGGACT TAGTACTTCT 
GTTGTGCAAA GACCAGGAGC ACAAGCCATA AAATTTGAAA CCAAAATTGA TTCATTTCAT 
ATTACTGGCT TACCAGATGA TTTCAAAAAA CCCCACCTAC TGTCTTCATT GGATGATACG 
TCACTCCTCC AGATTACATT TGAGATAAAT CCTTTAAATG AAACTGTTGC TCAGAGGTGC 
ACCATAGAGG CTGAGCCTCT AGAGATAATA TATGATGCAA GGACAGTCAA TAGTATAGTG 
GAGTTCTTCA GACCTCCGAA AGATGTCCAT CTAGCCCAAC TTACTTCAGT AACTTTGACA 
AAACTTGAAG AATTTCGAGC AAAAACAGCA ACAGGTTTGC TGTATGTTAT TGAAACACAG 
AAAGTTCTTG ATCTCAGAAT TAATGTAAAG GCTTCATATG TTATTGTCCC ACAATATGGA 
AATTTTAGCC CTACGTCAAA TCTACTTCTC CTGGATCTTG GTCATTTAAA GGTGTCAAGT 
AAGCGTCGAT CACTGCTGCC AGATGTGAGA CCCAGTGAGG CCAGCCTTGA AGATATAATG 
CACAGAGCGT ATGACTCCTT TGATATTCAG CTTACGAGCA TACAGTTGCT CTACAGTAGA 
GTGGGTGATA ATTGGAAAGA GGCACGAAAG CTCAATGTAT CTACACAGCA TATTTTGATA 
CCTATGCATG TCAATGTGGA GCTCTCTAAA GCCATGGTTT TCATGGATAT CAAGATGCCC 
AAATTCAAGA TTTCTGGAAA GTTACCTCTT GTTTCATTAC GAATTTCGGA TAAAAAACTG 
CAAGGCATTA TGGAGTTGTT GGGAAGCATT CCAAAGCCTG AACCTGTAAC TGATGTATCT 
GCCCCTGCCA GATCATTCCA GATTCAAGCA TCTGCTTTGC CAGTATCACA CATTTCTCAG 
AAGTTAATCC CTTTATTGGA ACAGCCTGTT ACTGAAGATG ACTCAGAGGA AGAATTTTTT 
GATGCACCAT GTAGTCCCTT GGAGGAGTGT CCTCAAGTGT CATGTAGAGA TAAATGTACT 
CGACAGAAAA AATTACAAAA GAAAGATTGT GTGATGAATC TTATTCAACT TAGGATGAGA 
TTTGAAGTAG CAGAGGTGTC CATTCAGTTT TATCACCTGG TGGGAGACTG TGAACTACCT 
GTGCTGGAAA TGGGTGCTTT AGGATTGGGA ACAGAAGCTG AGTTTAGAAC ATTTGACTTG 
AAAGGAAGTG CCTTTTTGAA AGAATTATGG CTAAAATGTC CAGAATATTT AGATGAAAAC 
AAGAAACCAG TTTATTTGAT CACGACCCTG GATAACACAA TGGAAGATTT ATTAACATTG 
GAATTCATGA AGGTTGAAAA AAATGCACCA AATTTGAATA GTACCTATAA CAATGTTTTA 
CAACTTATTA AGGTGAATTT TTCCTCTTTG GATATTCATT TACACACTGA AGCACTTCTG 
AATACAATGA ATTATCTTAA TAATATTCTT CCGGAATTAA GGGAAAAATC TGCCTCGGTG 
TCTGCTGCAG AGCCGGAAGA CAAAGGGGAC ATCATTAAAA AACTTGCTTT AAAGCTACCC 
ACGAATGAAG ATATTATTAC CTTGCAGCTT TTGGCAGAAT TATCTTGTCT ACAGATTTTT 
ATTCAGGATC AGAAACAAAA CATTTCTGAA ATTAAAATTG AAGGGCTTGA TTCTGAGATG 
ATTATGAAAC CTCTAGTCAC TGAAATAAAT GCAAAGCTAA GGAATATAAT TGTGTTAGAT 
TCTGATAAAA TGGCTATCTA CAAAAAGGCT CTTTATATCA CTGGAAAAGA AGTTTTCAGC 
TTCAAAATGA TTTCTTACAT GGATGCAACT GCTGGCTATG CATACACAGA TATGAGTGTG 
GTTGACATTC GGGTTCATTT AACTGTTGGT TGCATTGAAG TAGTGTTCAT CACAAAATTC 
CTGTATTCTA TATTGGCATT TATAGATAAT TTTCAAGCAG TTAAAGATGC CTTGGCTGAA 
GCAACTGTTC AAGCAGCAGA GATGGCTGCT GATGGTGTAA AGGAACTGGC TCGAAAGAGC 
TCTAGGTTTG CATTGGATGT TAACATCAAG GCTCCAGTTG TGCTCATTCC ACAGTCTCCA 
GTGTCTCAGA ACGTTTTTGT TGCTGATTTT GGATTAATTA CAATGAAAAA TATATTTGTT 
ACTGTAACAG AGACCCAGAG TAATATCCCA CCTGTAATTG ATTTGATAAC AATAAAGTTG 
AGTAAAATGA GACTATACAG GTCTCAGTTT AGGAATGATA CATACCAGGA AGTGCTGGAC 
CTGCTACTAC CTTTGAACTT GGAAGTGATT GTCGAACGGA ACTTATCCTG GGAATGGTAC 
AAGGAAGTCC CTTGTTTTAA TATAAAAGCT CAGCTAAAGC CCATGGAGTT CATTCTTAGT 
CAGGAAGACT TAACAACCGT CTTTCAAACA TTGCATGGCA ATATATGGTA TGGACAAGAT 
CTGAGTGCTC CATCTTCTGC AAATAAGGAC CCAGAGACCA TGACCAGTGG GGTCACCAGT 
CCTCCTGACC ATTCGCCAGC TACTGTGGTG ACCGCTGCTG TGGTGGAGGT GCACCCACAG 
GCTTCTCAAG CGCACACGAT GCTGAACGTG AGCTTCCAGA CCGATTACCT CACCATGGCA 
CTCTACAGTC CGGGGCCGGA TGAGGCCTCC TTTACAGATG TTCGCGATCC TTCTCTGGAA 
CTTGCTGAAT TTAAGTTGGA GAATATTATA AGTAGTTTAA AAATATATAC AGATGATTCT 
ACAGTCTTTT CTTTCTCAGT AAAAAACTGT ATTTTAGATG ATAAAAGGTC ACATGTCATG 
AAAGCCACTC CTAGAATGAT AGGATTGACT GTTGGCTTTG ACAAGAAGGA CATGGTGGAT 
ATAAAGTATA GAAAAATCAA AACTTTTGTT GTGACTGATG CAGTTGTCCA AGAAATGTAC 
GTTTGTGCGA GTGTGGAGTT TCTGATGACT GTTGCCCATA TCTTTTTTGA TGCCTACATG 
ACTAGTACGG CTTTAGAAAC CAGCGTTCAA ACAAGGACTA CTAGAGAAGC ACCTGCACAG 
GAATTAGGGA AGTGGGAAAT GAATATTCTT ATTAAAAATC CAGAGATTGT GTTTGTGGCT 
GACATGACAA GAAACGATGC TCCTGCCTTA GTCATTACGA CACAGTGTGA AATTTGCTGC 
AAAGGTGAAC CTACAAGCAA TACAGTGACT GCTGCCATTA AAGATCTCCA AGTCAGAGCG 
TGCCCATTTC TTCCAGTCAA GAGAAAGGGC AAAGTCACCA CTGTTTTGCA GCCTTGTGAC 
TTGTTTTATC AGGCTACTCA ATTAGGTAGA GATCCACAAA TGATTGATAT ATCAGTAAAA 
TCTCTTACGC TAAAGGTTTC ACCAGTTATC ATAAATACCA TAATAACTAT TACTTCAGCA 
CTTTACACAA CTAAGGAAAC TGTCCCAGAA GAAAACACTT CTAATATAGC ACATTTATGG 
GACAAGAAGG ATACAAAGAA TCTAAAAATG TGGTTTCTTG AAGAATCAAA TGAAAGTGAA 
AAAGTAGTTC CCACGAATGA AGTGATGCCG GGAGGAGAGA CACTGAACTT GCGTATTGAT 
TCTATTTTTA TTGTTCTGGA GGCTGGAATC GGTCATAGAA CAGTGCCGAT GCTGTTGGCA 
AAGGCGTGCT TCTCGGGGGA AAGCAAAAAC TGGCTCTCTC TAATCAATCT TCACTGTCAC 
CTGGAACTGG AAGTTCACTA TTATAATGAA ATGTTTGGTG TTTGGGAACC TTTACTGGAA 
CCCTTAGAAA TTGATCAGAC TGATGATTTT AGACCCTGGA ATCTGGGCAT CAAGATGAAA 
AAGAAAGCAA AAGAGGCCAT TGTTGAGTCT GACAGTGAAG CAGAAAACTA CAAAGTACCA 
GAATATAAGA CTGCCATAAG TTTTTATTCT AGAGACCAGT TAAACATTAC ATTATCCAAA 
TGTGGCCTTG TGATGTTAAA TAATTTAGTT GAGGCGTTTA CTGAAGCTGC TACTGGATCG 
TCATCTGTCT TCCTAAGAGA TCTAGCACCA TTCATGATTT TCAATTCTCT TGGACTTACT 
GTCTCTGTCT CACCAAGCGA TTCTTTTAGC GTTCTCAACG TTCCTTTGGC AAAATCATAT 
GAACTGAAAA ATGATGAGAG TTTAAGTATG GATTATGTAC GAACGAAGGA CAATGATCAT 
TTCAATGCAA TGACCAGCCT AAGCAGCAAG CTCTTCTTCA TTCTCCTTAC GCCTGCTAAC 
CACTCTGTCG CTGACAAGAT CCCTTTAACA AAAGTGGGAC GCCGTCTGTA CACTGTAAGA 
CACAGAGAAT CTGGGGTCGA AAGATCTATC ATTTGTCAAA TTGACACAGT TGAAGGAAGT 
AAGAAGGTAA CAATCCGCTC ACCAGTGCAG ATTAAGAATC ATTTTTCAAT CCCAATTTCC 
GTTTTTGAAG GAGATACTTT ATTGGGAATT GCTTCACCTG AAAATGAATT TAACATACCA 
TTAGCATCTT ACCGATCATC CCTTTCCCTG GTGCCAGAAG ACCAGGACTA CCAGCTATGT 
GAAGGAATCG ACTTTGAAGA AATCATAAAA TATGATGGAC AGCTTCTGAA GAAGAAATGT 
AGATCTACAA ACCCTTCTAA GAAATCATTT GTTATTAATA TTGTCCCAGA AAAGGATAAT 
TTGGCTTCCC TGTCAGTGTA TTCGGAAGAT GGTTGGGACT TACCATATGT CCTGCACTTG 
TGGCCACCCA TCCTCATCCG GAACCTTCTT CCCTACAAAG TTGCTTATTA CATTGAGGGC 
ATTGAGAACA CTGTTGTTAC TCTAAGTGAA GGACATTCAT CACAGATCTA TAATGTAGAA 
ATGGACCAAG CCAAGCTACA CTTAAAATTA CTCGACTATC TTAATCATGA TTGGAAAAGT 
GAATTTTATA TAAGATCTAG TCAACAAGAC ATCAATTTCA TCAACTTTAC CTGTCTCACA 
GAAATGGAAA AGAGTGACTT GGATATTGCT ATCCATATGA CTTACAACAC TGGTCAGACA 
GTTGTGGCAT TTCATAGTCC GTATTGGATG GTCAATAAAA CCAATCGGAT GCTACAGTAC 
AAAGCAGATG GAATTCACCG AAAGCATCCA CCTAATTATA CAAAACCAGT TCTCTTTTCC 
TTTCAGCCAA ATCACTTTTT TAACAACAAT AAGGTTCAGC TTATGGTAAC TGATAGTGAG 
CTTTCAGATC AGTTTTCAAT TGATACTGTT GGTAGTCATG GAGCCATTAG ATGCAAAGGT 
CTGAAAATGG AATACCAAGT TGGTGTCACT ATAAATCTCA GCAGTTTTAA CATTACCAGA 
ATTGTGACAT TTATTCCGTT TTATATGATT AAAAACAAAA GCAAATACCA TATATCAGTA 
GCAGAAGAAG GAAGTGATAA ATGGCTTTCT CTTGACTTAG AGCAGAGTAT CCCCTTTTGG 
CCTGAGAATG CCTCCAATAT TCTTCTTATT CAAGTTGAGA GGAGTGAAGA TCCTCCAAAA 
AGGATATATT TTAACAAGCA AGATAATTGT ATTTTATTGA GACTGAATAA TGAGCTTGGA 
GGTATTATTG CAGAAGTTAA TTTGGCTGAG CACTCTACAG TTATTACATT TTCAGATTAC 
CATGATGGAG CAGCTACATT CCTATTAATA AATCATACCA AGAGTGACCC TGTTCAGTAC 
AACCAAAGTT CTCTCGGTGA AATAGAAGAT TCTCTTCCTC CTGGAAAAGC AGTCTATTAT 
ACATGGGCTG ACCCAGTGGG CTCTAGAAAG CTGAAGTGGA GCTGTGGGCA AAGCTATGGT 
GAAGTAACCC ATAAGGATGA TATGATGACG CCCATAAGTG TGGGGAAAAA AACCATTTAT 
TTAGTGTCGT TCTTTGAAGG CCTGCAGCGT ATTATTTTAT TCACTGAAGA TCCAAGAGTG 
TTTAAAGTAA CGTATGAAAG TGAAAAGGCA GAGTTAGCGG AGCTGGAAGT TGTGCTGGCG 
TTACAGGATG TCGGGATTTC ACTTGTCAAT AACTATACAA AGCAAGAAGT AGCCTACATA 
GGCATTACAA GTTCTGATGT GGTTTGGGAG GCAAAACCAA AGAAGAAGGC CAGGTGGAAG 
CCCATGAGTG TGAAGCACAC AGAGAAGCTG GAGAAGGAGT TCAGGGAGTA CACAGAGGCC 
TCGCCCTTGG AGGACAAAGT GGTCGAGTTG GACAACATTC CGGTTCGTTT AACACCTAGT 
GGCAATGACA TGAAAATTCT GCAACCACAT GTGATACCTG TCCGGAGAAA TTACCTGCCA 
GCACTAAAAG TAGAATATAA CACATCTGCA CATCAGTCGT CATTCAGAAT TCAGATTTAT 
AGAATACAGA TCCAAAATCA GATTCATGGT GCTATTTTCC CTTTCGTGTT TTATCCTATT 
AAACCTCCAA GATCTGTCAC CATGGACTCA GCACCAAAGC CGTTTACAGA TGTCAGTATT 
GTCATGAGAT CTGCAGGACA TTCCCAAATA TCACGCATTA AGTATTTCAA AGTGTTGATT 
CAAGAAATGG ACCTCAGTTT GGATCTTGGA TTTGTGTATG CCCTAGCAGA CCTTGTCACA 
AAAGCTGAAG TGACTGAGAA GACAGAGGTG GAACATTTTC ATAAGGATGT AGAAGCATTT 
GAACAAGAAT ATGAAGTTGT TTCATCAGTA GATCAATCAC AAGTCAATCT CTTTGAATAT 
TTTCATATAT CTCCTATCAA GTTGCACTTG AGTGTTTCCC TGAGCTCTGG TAGAGATGAA 
GCGAAAGACT CAGAACAACA TGGAGGACTA ATTCCTGTTC ATTCGTTAAA TCTTTTGCTG 
AAGAGTATTG GTGCCACCCT CACAGATGTA CAAGATGTAG TTTTTAAGCT TGCCTTTTTT 
GAACTCAACT ACCAGTTCCA TACAACATCT GAATTGCAAT CTGAAGTAAT AAGACACTAT 
TCCAAACAGG CTATTAAACA AATGTATGTA CTCATTCTTG GACTTGACGT CTTGGGAAAT 
CCCTTTGGCT TAATTAGAGA ATTTTCAGAA GGAGTAGAAG CGTTTTTTTA TGAACCTTAC 
CAGGGGGCTA TCCAGGGTCC TGAAGAGTTT GTGGAGGGAA TGGCGCTAGG ATTGAAAGCA 
CTAGTTGGTG GAGCCGTTGG TGGGCTAGCT GGTGCTGCCT CCAAAATCAC CAGTGCTATG 
GCTAAAGGAG TAGCGGCCAT GACCATGGAT GAAGACTACC AGCAGAAGAG GAGAGAAGCA 
ATGAATAAGC AGCCTGCTGG CCTTCGAGAG GGCATCACTC GAGGAGGAAA AGGCCTTGTC 
TCTGGTTTTG TAAGTGGCAT TACAGGGATT GTTACAAAGC CAATTAAAGG TGCTCAAAAG 
GAAGGAGCAG CAGGTTTCTT CAAAGGTGTT GGAAAAGGTT TAGTTGGAGC AGTGACAAGG 
CCAACGGGTG GCATCATAGA CATGGCCAGC AGTACCTTTC AGGGAATCAA AAGAGCTACA 
GAGACTTCTG AAGTAGAGAG TTTACGACCT CCCAGATTCT TTAATGAAGA TGGAGTCATC 
AGACCTTACA GGTTGCGCGA TGGTTCAGGA AATCAAATGT TACAGAACAT TCAGGCCTAC 
AGGGAATGGA TTCTGACTCA CAGTAGCAGT AGTGAGGAGG AGGAGGAGGA GGAGGAAGAG 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Phenotypic abnormalities in a chorea-acanthocytosis mouse model are modulated by strain background.
Biochemical and biophysical research communications472(1)118-24(2016 Mar)
Sakimoto H,Nakamura M,Nagata O,Yokoyama I,Sano A

Chorein, the protein responsible for chorea-acanthocytosis, interacts with ?-adducin and ?-actin.
Biochemical and biophysical research communications441(1)96-101(2013 Nov)
Shiokawa N,Nakamura M,Sameshima M,Deguchi A,Hayashi T,Sasaki N,Sano A

Subcellular localization and putative role of VPS13A/chorein in dopaminergic neuronal cells.
Biochemical and biophysical research communications419(3)511-6(2012 Mar)
Hayashi T,Kishida M,Nishizawa Y,Iijima M,Koriyama C,Nakamura M,Sano A,Kishida S

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Vps13a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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