Vps13 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps13
Entrez Gene ID	35693
Full Name	Vacuolar protein sorting 13
Synonyms	BEST:LD02743,CG2093,Dmel\CG2093
General protein information	Preferred Names Vacuolar protein sorting 13 Names CG2093 gene product from transcript CG2093-RB CG2093-PA CG2093-PB Vps13-PA Vps13-PB vacuolar protein sorting 13
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 43D7-43E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273852.1	NP_001260781.1	vacuolar protein sorting 13, isoform B
NM_136455.3	NP_610299.2	vacuolar protein sorting 13, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS13A	NP_150648.2
	LOC705323	XP_002808150.1
Canis lupus familiaris (dog)	VPS13A	XP_533522.3
Bos taurus (cattle)	VPS13A	XP_002689716.3
Drosophila melanogaster (fruit fly)	Vps13	NP_001260781.1
Saccharomyces cerevisiae (baker's yeast)	VPS13	NP_013060.1
Eremothecium gossypii	AGOS_ACL064C	NP_983340.2
Magnaporthe oryzae (rice blast fungus)	MGG_06537	XP_003717004.1
Rattus norvegicus (Norway rat)	Vps13a	NP_001094445.2
Kluyveromyces lactis	KLLA0E00705g	XP_453978.1
Pan troglodytes (chimpanzee)	VPS13A	XP_001148547.1
Mus musculus (house mouse)	Vps13a	NP_766616.2
Xenopus tropicalis (tropical clawed frog)	vps13a	XP_002937242.2
Gallus gallus (chicken)	VPS13A	NP_001264765.1
Neurospora crassa	NCU05837	XP_960097.1
Danio rerio (zebrafish)	vps13a	NP_001112365.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005082	XP_558472.3
Caenorhabditis elegans (roundworm)	T08G11.1	NP_740900.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila Vps13 mutants are suitable to investigate the function of Vps13 in the brain, to identify genetic enhancers and suppressors and to screen for potential therapeutic targets for Chorea-Acanthocytosis
Title: Drosophila Vps13 Is Required for Protein Homeostasis in the Brain.

The following Vps13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09160	NM_136455.3 Latest version!	Drosophila melanogaster vacuolar protein sorting 13, transcript variant A (Vps13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa09160	NM_001273852.1 Latest version!	Drosophila melanogaster vacuolar protein sorting 13, transcript variant B (Vps13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps13 ( NM_001273852.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260781.1 Drosophila melanogaster vacuolar protein sorting 13, transcript variant B (Vps13), mRNA.

Price & Availability↑

CloneID	OFa09160
Clone ID Related Accession (Same CDS sequence)	NM_001273852.1 , NM_136455.3
Accession Version	NM_001273852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 13, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCTTCG AGGCGGTGGT GGCCGATGTG CTGAACAAGG TGCTCGGCGA CTACATCGAG 
AACTTGGACC GGAACCAGCT GAAGATTGGC ATTTGGGGAG GCGATGTCGT CTTGCAGAAC 
CTCAAGATAC GGGAGAATGC CCTGGACGAG TTGGACCTGC CCGTACAGCT TATCTATGGT 
TACTTGGGCA AACTAGTGCT TAAGATCCCC TGGAAGAATT TGTACAGCCA GCCGGTAATT 
GTCAACATTG AGGATCTTTA TGTCTTAGTT TCACCCAACA ACAATGTCCA GTACAACGCC 
GAAAAGGAGG CCAAATATGA GATGGACCTC AAGAAGGCGG CGCTTGATGC CCTGGAGGCA 
GCTCGCAAAA AGGAGTTGGA AATGGATCAG CCCAAAGCAG ATGCAGGATT TGCCGAAAAG 
CTAACGGCGC AGATCGTTAA CAATCTGCAG GTGCAGATAA CCAACGTTCA TCTGCGATAC 
GAGGATACCA CAACGACGGG TAGTCCCTTC TCCTTCGGCA TATCCCTGCA TGAGCTCGAA 
CTTTACACCA CCGATTGCGA CTGGGAGAAG TGCTACATGG CCCAGCAGGC GTCGCAGGTC 
TTCAAAATAG CGAATCTCTC ATGTCTTTCC GCCTATTTGA ACTGTGGTGG ACAACTGTAC 
GCGAACAACA AGAGCGACCT TTCACAGCAG TTCAAGACGA ATATTGCTTG CAAGGAAACA 
AAACCCAACT ACAACTATGT GCTGGGTCCT ATCTCTTGCA ATGCCAAGCT AAAGCTAAAC 
ATGAACCCGG AGCTCGATGA TCCGCCATTT GAAAAACCTA AAATCGATCT TACGCTGGAA 
ATGGAAAAGC TTAATGTCGG CTTGACAAAC ACACAGTTCG ACAATCTCAT GAAGCTTGGA 
GACGCTATGA ACCGCCAGCA GTTGGGCATA CCGTACCGGA AATATCGTCC CTACAATATA 
CCATACAAAG GCCACGCCAG GGACTGGTGG CACTTTGCAA TAACTTCTAT TCTAGAGGAG 
GAAGTAAGGA AACCCAGAGA GAGCTGGACT TGGGGACACA TCAAGACCCA CAGAGAACGC 
TGCAACACTT ATGCACAGAA ATACAAGGAG CAGTGCTTAA GCAAAAAACC GTCAGCCGTG 
CTTACGGAAA CCTGCCGTTT GCTAGAAACG GAGCTGGACG TTTTTAACTT GCTGCTAATC 
CGGCAGAGGG TCAACATCGA GATAGCCAAG CAACGAGAGG CAGTGCCTGA ACAAAAGTCG 
GGCTGGTTCA GTGGTTGGGG ATGGGGCGGC GGAGCCAAGA AGGATGACCA AACCACCAGT 
CAAAAACTTG TTGAGAAGTT TGAAGCGGCA ATGACCAGTG AAGAGAAAGA GAAGATGTAC 
AGAGCTATTG GTTACCAAGA GAACGCCAAG CCCACAGACC TTCCAGAGAG CTACGAGGCC 
ATCAGGATGA ACTTTAAACT GATAGCCTTA GAGGTCGGCC TTTACAAGGA TGAGCGTAAT 
AGTTCCGCGG CAACGAAGGA CTTCCATGAG CTGCCGTCGC TGGTCCTTCT TAACTTTTCT 
ATGGCCACCG CGTTGATCAC CCAACGGCCA GCTGCCGAAG CCATCAGCAT TATTGCTGGC 
ATGAGAGAAA TCAAGGTAAC TGGTCTCACG CGCAACGATT ACACGCCTTT ATTGGTTGAG 
TCAAAAATCA CCGACGAGTT CAACCTGCTG GAAGTGTTTT TCGAGACAAA CCCACTGGAC 
AAGTTGTGCG ACCAGCGAGT GAAGGTTGTG GCGCGCCCGT TGCAGATAAC GTATGATGCG 
CCAACCATAT TGGCCCTGAT AAACGCATTC CAGACGCCTG GCGATGTCAC GCTCTCCAAG 
TTTGAGGATG CTGCCAGCAC GAAAATCTCG AACTTCAAGG AGCGTTCAGC CACTGGAATG 
CAGTACATGA TCGACAAGAA GGCAGTGCTG GATGTGGACA TCCTGTTAAT GCCAAACATC 
CTGGTCGTAC CACATAAAGG AGTCTACGAT GCGGGCAACG TATCCCTGCT AGTAGTTAGC 
ATGGGCCAGG TGCACCTCTC CAGCCAACCG CGTAGGGAGT CGAACAAGCT GCAGCATCTT 
TTCTCAGCCG GCGAGGATAA GGATGAGATC CTCAAGACGG TAATGGAAAA TGCTTATGAC 
AGGTTTACAG TCGCAGTTGA CGATGTGCAG ATGCTGGTCG TGCGAGCTGG AGAGCCGTGG 
CAAAATGCGC TAGCGGAGGC AAACTCAACG GAGATGCACG TTTTGCGCCC CGTCTCCCTA 
AAAGTAACTG CCGCACTATG TGTGGTTGAC AATGATCCGC GTCTTCCAAA TATTAAAGTG 
GACATCGACT TGCCTGCGAT CCTAGTTAAT GTCTCAGAGG ATCGCATTTT CCTCGCCATA 
AAGGTGGCAA CAAGTATTCC CCTGCCGGAG CAAAAGGAAC CGGCTAGTAG ACTCACACAG 
ACGAACTCGC GCAGCTCAAT GTCCATATCC AATTTCATCA ACAAGGAAGT GAAAAAGATT 
GGGCCTTCGG CATCGGGGAG CTCTGCCAGT AAGGATCCGT TGCTGGACGA GATCATTCAG 
TACACCAGTC TCGATGTAAA TTTTTCTCTT GGAGAAATCA ACTTCGTTTT GTTCCAGTCC 
AGTAGAAAAT GCGAGACAAG CCCCGATGTT TCTATTGAAT TTCTGACTCC TGATGGTGAT 
GTCCTTCCTT CTCAGTTGAC TGAGAATATC CAGGAGCCTA TTGAAGAGCT ACCCCCAACG 
CCACCTCAGC AGATTCTCTC AATCGACATT CGACGCCTGG AGGCCCACTT TGTGTCCAAA 
ACCTATGAAT CGGTGGCCAC AGTCAAACTT GGAGATATCA ACCTGCGACA GTATGACTGC 
CAGGACAGTG ACATGGATGT ACTAGATGTT ATCTATACGC CGAAACAGGA GAATAGTTCC 
AATTACCTGT TCACCGTCTC GTGCACAATT GCAGACAAGT CGTCGCCAGA GTTCAGCACC 
AAGTACAACT CCACTGAACA GTTGGTCGTG GCTAACTTCG AGGTACTTCA AATCGTGCTG 
CACCAGGAAT GCCTACAAAG GATTATGGAG GTCGTAAACA ATTTCCAAAG AAACCTAGAT 
CTTGTACTAT CAAGTACCCG ACCACGGGAT CGGATGGGCT CCATTGGGGG AGGAGATGGC 
ATTAAACGTA CGCTTAATGT TATTCTTGAG GACACAGAGG AAATAATGAC CACCGACCAG 
ATGAAGCGGC GAAAGAAGAC GCGCCGCACT CACGTCGTAG AGACGGTGAA AGTTCGTGTT 
ATCGCTAATT TGGATCAAGT GGGTCTGGTG CTGACCGGCC GAAAGCGGCC GATAGCAGAG 
ATGAATGTGA AGAAATTCGT GTCCAGTCTG ATCATAAAGT CGTCCTACAC GGAAGTCAAC 
ATTGGCCTTA AAGACATTCA GGTGTTGGAC CTAAATCCTT ACACTATTCA CAAGAATATT 
CTGAGTATTG TTGGCAAGGA CGCTTTCAAC TGCCAGATTG TGATCTACAA CAAGGAGGAG 
ACCCAAGACT ACAACTCTGA TGATATGAAA ATAACCGTGG ACATTGGCTG CATGAAGATC 
ATCTTCCTCA ATTGGTTTGT GGCAGGTGTT ATGAACTTCT TAAACAACTT CACAGCGGCC 
CAAGCAACCA TATCGCAGGC TGGTGCTGCG GCTGCCGAGT CCGCTCGCCA GAAGGCAATG 
GATGCCTATG AAACGGCCAC TCGTATGAAA CTAAATATCC GCATCAAGGC TCCCATTATC 
ATTGTGCCAA TTGGGTCACA GGACAGGAAT GCCTTGCTGC TCGATTTGGG CTTACTGGAG 
CTAACGAATA ACACGGTGGA GGTGGCGGTG GCCGAAGAGG AACGGCTAGC TGTTATAGAC 
GAGATCAAGT TGCAAATATG CGATGTTAAG ATTTCCAAAA TTGTCTTATT AGATGGCAAC 
GAGTCAACGG TTGATGAAGT GGACGCGGAA GTGGGTTTCC TCTCTAAATT CAATATGATG 
AATCCCATGA GTTGCACCTT ATCAATCACG CGCAATTTGT CATACACATG GTACCGGGAT 
GTGCCCGAAC TAAATCTTTC CGGTCGATTA AAGTCCATTG AGCTCACTCT ATTCGCGGAT 
GATTATGCTT TGGTAATGCT TGTTCTCAAT CGCAATTTAA ACGAGGGCTT GGAAGAGTTC 
CCTCCAAGCG AGGAGGCGCC GCAGGAAGCA CAAGTTAGAC CCGAAAGACG CAACTCTCGT 
GCGGGACGTT TATCTCGTAC CGTTCAAGTT TCTCCGATAA GGGAAAAGAT TCACGAATCA 
ATCAAGTTTA ACTTTCAGTT TGACGGTGTG GTCATAAATT TAATGGAGGG TGAAGGAGCA 
GGACTCGCCA GGTTTGGAAT ATATTTCCTC TCCGTTAAGG GCACCAAGTT GGACAACGGC 
ACACTGAGCA CTTCTGTTGT ATTGTGCAAT ATTCAAATGG ATGACATGCG AAGTAATTCC 
AAAAGCCAGA TCCGACAGTA CTTGAGTCGC AAGGATTGGG TTCAGCCTAA GTTGGATACA 
GACGAGATCA TCGATGCCTG TTACAACGAG CGCAACTTCA TGGTCGATGT GACGGCTATA 
ATAAAAGAAG ATGACACGTT CGCTGAGGTC CGTGTGCGTG GCTTCGATCT GATCGTCTGC 
ATCGACTTCC TGCTGAAACT GACTACGTTT CTTACCTTGC CGCCTGAGGA GAATCCGCGC 
GAGTCTGTTT ATATAAAGCC AGCGCCTGTT TCGGAAACTG CTCGGGATAC CAAGCACTCT 
ATTCGCTCCT CCGCCATATT AGCCGCCCAA GAGCTGGTTC CTGTCGAGTC ATCCAGTCAC 
GAAGTTCCAA ACCGAAAAAT GAATTTAATA CTGCATATTG ACGAGCCAGA TATAATATTA 
GTGGAAAATC TGGAAGATCT TAATACCAGT TGTATTATTT TCAATGCTCA AGTGCATTTG 
AATTACCGCA GCATAAACGA CAAACAGATT GTAAACGGAC AGATCGATGC TCTGAAGATG 
TACATGTGTG CTTTCCTACC AGAGCGGCGA GAGATGACGC GTCATTATAT CCTTCACCCG 
TGCGTTATTA GCCTGCAAGG ATCAACTCCC GAAGAAGAGG GCATGCACAT TAGCCTAAAG 
CTCAGTGACA TTATCATCAA CGTGTCGCCG GCCACCATCG AGCTGCTAAA CAAGGCAATG 
CTGAGCGTCT CCAGTGGCAC CATGACCAAG TGCGCTATTG CCGAGGAGTC GCGGAACTAT 
TCGAATCTGT GGCATCAGCA CCACTTCCAT AGCCGCACGT ATTGGTTCAC CAAGGTGGAA 
CAGGGCGTCG ATGCCCTGGA GGCTGAGCAG AGAAGCGTCA GCACTGACAA CGAAAAGCAG 
AAGACAGAAA AGTGTGTAAT TGAAATTCCC AGCATTACGC TCGTGATTGA GTCCGGCGTA 
GGCTACTACA CGAAGCCACT TATTTCGCTA GACACCCGCA TTACTGCCGT TTTCAACAAT 
TGGAGCCGCA GTCTCACCGC TCATGGCTCG TTGACACTAA ATATGAATTA CTATAACCAA 
GCCCTTGCCG AATGGGAGCC TATTATTGAG CTGAACGAGG TCATTGGTCG CAACGGCGTT 
CGTGAATACA CGCCATGGGA GCTTAAGTTC GAAATGGGCA TGGAGAAGGT GCAGAGCGAA 
TTGGAAGACG ATGCTGAACA GCAGGCGATG CACATGAACA TCCACTCGGC AGAGACGCTG 
GAGATTACGT TGAGCAAGAC GTGCCTGGGT CTATTGAGCG AACTGGCCGA GGCCTTCTCG 
CAGGCTATTG ACCAGAACGG TCTTACCAAG CCAGACATCG TGGCTCCTTA CGTGCTGGAA 
AACGACACTG GCTTCGATGT TAACCTGAAT CTGCGGAAGG GCATCTTTAC ACTGCACGAA 
GTGCACCGCG GCGGCACACC TGTGGGTGCA AATAGTACCC TTCTTATGGT GGCCCAATCT 
GAAGAGGTCG ATCCCAGCGT CATAAAAACC TGCACCATTT CGACGGGCGG AAGGGCATAT 
CTGCAGACTA AGGATCTGAG CACGTTATCA GAAGAGGATT CCGAGGACTA CACTCTTTAT 
GTGACGATTG GAGATATCAA CAAGGAGATC GCACTGCCCG TTTCCAAGTC GGACACGCGT 
TTCTTCAACC TGATGCGCAG TACCTCTCAC GAGCCTTGGG GCATTATTTC TGAAGTAAAG 
CAGGAGTACG GCACAACCAA AGTCAATATC CATGGTGTTG TTAGTGTTCA TAATCACTTT 
ACGACCGGCT TGAATATTTA TCGACGCAAT CCTGCCCCTA CTGCACAATG TTTCGAGGAT 
ATTTTCGTCG GACGTGTGCG TCCCGGCGAA GTGTTCCATG TACCACTGCA CGCCATATAC 
GCGGAATCCA AGGATTTATT CTTTTCCATG CGCGGCTACA GAAGATCAGT GCAGGGCATT 
TCGTGGGCTT CCAATCCCTC GGACCTTAAC TATTCGCATC AGTTGCATTG TGATCCGACC 
AATACGTTCG AACCGCTTAT CATGAACGCT CGAAGATCAA AATCGGAGGT GTACTTCGAA 
AACACCAACA AGTATACATT GCTAAGCGCC TTCTACACGA TTCATCTGCG CCCTCCACTC 
TATCTGCGCA ACTCTCTGCC CATTAATATC CAAGTGTCAG TTGCCGGCTG CTCTGTCCGA 
AAAGAGGATG GCCTGGATGC ACAGTCGTCG CAGCGCTTTG TTGACAGAGG CTATCGTAAG 
GAAGACTTTC TGGACTATGG AGAAAAGCCT GTAAATTCCG GCGACGTGCT CCACCTGCCG 
ACCGTTCGAT TGGCCTCGAA AGGCAAGGAA TCGAAGAGTT TCCTAGTTGT GCGGCTGGTT 
CAATATCTGG AGAAAGACTG GTCCTGCGCT ACCGAGATCT GGGATTACAC TGATGATGTT 
ATTACCTGGA CGTTTTCATC ATATGACTCG GAAATGAAGG TGGACATGGA TCTTTATGTG 
AAAACAGAGA ACCGACATGG CAGCTTGATG CTTACGCTTT TTAGCCCTTT TTGGATGATC 
AATAAGACCG GCATGATGCT CACTTACAAA TCGGAAACGA CCTCCGTGGA AGTTCTCTAC 
CATCCTCCTG AGTATTCTGG TCCCATTTTG TTCACTTTCC GGGATAAGCT ATTCTTTGAC 
AAGAAAAAAG CTTCAATTCG CATAGACAAT GGTCAATGGA GTGAAAAGAT TCCATTGGAC 
GTGGCGGGTT CGGTTGGCGA AGTGATCTGC TTTGCGAACA ATCAGAAATA CCCTGTCGGT 
GTTCACAACC ACTTGACACA GAACTCTTTG ACCAAGCAGA TAACGTTCAT ACCCTTTTAT 
ATTGTGTGCA ACAAGTGTCA CTTTGATATA GAACTGCAGG AGCAGAGTCG TCCAGCCGAT 
CCATGGCTCC ATCTCGAGCC AAATGAGATG GAACCTCTCT GGCCGAGAAA CGACACAAAG 
AACAATTTGG TGGTGCGCGT GGATGGCAAG ATTACACCGG CCTTCGACTT TACGGAGGTG 
ATCTGTACGC TCCTGAAACT GGAGGACAGC AAGTACGGCG GTATCAATGT GGACGTTCAG 
ACAACGGAAG GCGGCGTCTA TATCACCTTT ACGGACTATA AGCCAGCCGA TGCGCCTGGC 
CTGCTCATCA ATCACACTGG CAAGCAGATT GTTTACCACG AGAAGGGAAC CAAAAACGAG 
CATATCCTAA ATGCAAAGAG TACTATAATG TATGCCTGGG ACGATCCTAC AGGTCCAAAA 
ATGCTTGTGT TCGGCACAAA CAAGGAAGAG ACGGATCTGA AACGAGACGG AATTGGCGAA 
GTGATCATGC AAGACGGCGG AAAGGTTCTA TGGGTATCAT TCCTCGACGG CCTGCAACGT 
GTGCTGCTCT TTACGGAAAA CGAATCGATC GCCAATCGTA CGGAAAGCAC GGCCTCGTTA 
CAGTCTATTA CCCAGAGCAT TGATCTGCGT ATTCACGGCA TTGGCTTGTC AGTGATCAAC 
AACGAGACGG GCCTTGACAT ACTTTATCTG GGAGTTACCA GCTCCGGCAT TATATGGGAG 
TCAAAGAAAG TGACCAAGAA TCGCTTTAAG GAGTTAACCA TTAACGAAAA CGCGCTTTTG 
GAAATTGAGT ATCAAAAATA TCTAGTTCAC AAGAGTGTCA ACGATGTGCA GACCTACAAG 
CTAGATAACA AGTTCCCGAT CGACTTCGAC CTTATGATCC TCAAGAAGAC GGTGGAGAGA 
AACTTAAGAC GCAGTTTCTA TCCAGCGATT TGGTTGTCCC GCAAGTCGTC GCCATTCCAA 
AGCCAGCTGC ATGTGAAAAT TAATCGTATA CAGGTGGATA ATCAGTTCCT GGATCCAATA 
TTCCCTGTGG TGTTGGCGCC AATTCCACCC CCGAAGAGTG TGGCAAGTAC CACCAGTCTT 
AAACCATTTA TCGAGTGCAG CATGGTGCAG CGCATCATGC CGAACTCAAC TGTGCGTCAG 
TTCAAGTACG CCAGGATACT GATCCAAGAG TTCCTCTTCA AAGTCGATTT GAACTTCCTT 
ACTGCCATCG CCGAAATGTT TGCCAAGGAG GTCTCGGACG AGGCAGCAGC TAAGCAGTTC 
CGTCAGGATG TTGAATCAAT TGAGCTCCCG CTTTCAGCGT TCTTTGAAGA GCACTCGTTG 
GAGGAGCAGA AGAGCTTTTA CGATAACCTG CATTTGGGTC CGCTCAAGAT CCATGTCAGC 
TTCTCGATGG CCGGGTCGGA CACAAAGGCG TTGCCCGGTT TCTTGGGCTC ATTGGTCCAG 
GGCGTGGGTG TGACTCTAAC GGACGTAAAC GATGTTGTTT TCCGTCTGGC CTTCTTCGAG 
CGCGAGTATC AATTCTTCTC CCAAAAGCAG CTAATCAACG AGATCACCTC ACACTACACT 
GGCCAAGCGT TAAAGCAGCT GTATGTGCTC GTCCTCGGAC TTGATGTCCT GGGCAATCCC 
TATGGCCTAG TGGTTGGTCT TAAGAAGGGC GTCGAAGACT TGTTCTATGA ACCGTTCCAG 
GGTGCCATCC AAGGTCCTGG AGAATTCGCC GAGGGTTTGG TCCTGGGCGT AAAGTCTCTT 
TTCGGCCACA CTGTGGGTGG TGCTGCGGGA GCGGTTTCGA AAATAACTGG TGCTATGGGA 
AAGGGTTTGG CTGCGCTGAC CTTCGACGAG GACTATCAAA AGAAACGACG ACAGGGTATT 
CAAAACAAAC CGAAGAACTT CCACGAAGGA TTGGCCCGCA GCAGCAAGGG TCTAGTGATG 
GGCTTTGTGG ACGGTGTCAC TGGCGTTGTA ACCAAACCAG TGACTGGGGC TCGCGATAAC 
GGGGTCGAGG GCTTCTTCAA GGGCCTGGGT AAGGGTGCAA TTGGCCTGGT TGCTCGCCCC 
ACCGCTGGCG TGGTTGACTT TGCCAGTGGC AGCTTCGAGG CTGTGAAACG GGCGGCGGAT 
GCAAGCGAGG ATGTGAAGCG TATGCGACCG CCGCGTTTCC AGCACTACGA CTTCGTGTTG 
AGACCCTATT GCTTGATGGA GGCGACAGGG AATAAAATAA TGAAGGAAAC CGACAAGGGT 
AAATTTGCCA CCACAGATAA CTTCATTCAC TGTGAGGAGA TTATCCAAAA GTCCGAGTAC 
TTGGTGGTGA CCAATTACCG GGTGATGTAC GTGCAACGTA ACGAGATGTT CGGCGTTTGG 
ACGTCTCTTT GGTCCTACCT ATGGAACGAG ATTAGCTCCG TGGCTGCAAC GGCTCGCGGT 
GTCCAGTTCA CAGTAAAGAC GGATGGCAAG AAGGTACTTG GTCTGTTTAG CAGCAAGGAG 
TCACCTCGAA AGTTAGTGCT CGTGGCAGAT GAGCGCAAAC GTGACGCTCT TGTGGACATT 
ATAGAATCTC AGCGCTCCGA TCCCAATCCG TTAAGGGCCA CCATCGCCTA TCCGGCTCAT 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260781.1
CDS	311..10276
Translation	MVFEAVVADVLNKVLGDYIENLDRNQLKIGIWGGDVVLQNLKIRENALDELDLPVQLIYGYLGKLVLKIPWKNLYSQPVIVNIEDLYVLVSPNNNVQYNAEKEAKYEMDLKKAALDALEAARKKELEMDQPKADAGFAEKLTAQIVNNLQVQITNVHLRYEDTTTTGSPFSFGISLHELELYTTDCDWEKCYMAQQASQVFKIANLSCLSAYLNCGGQLYANNKSDLSQQFKTNIACKETKPNYNYVLGPISCNAKLKLNMNPELDDPPFEKPKIDLTLEMEKLNVGLTNTQFDNLMKLGDAMNRQQLGIPYRKYRPYNIPYKGHARDWWHFAITSILEEEVRKPRESWTWGHIKTHRERCNTYAQKYKEQCLSKKPSAVLTETCRLLETELDVFNLLLIRQRVNIEIAKQREAVPEQKSGWFSGWGWGGGAKKDDQTTSQKLVEKFEAAMTSEEKEKMYRAIGYQENAKPTDLPESYEAIRMNFKLIALEVGLYKDERNSSAATKDFHELPSLVLLNFSMATALITQRPAAEAISIIAGMREIKVTGLTRNDYTPLLVESKITDEFNLLEVFFETNPLDKLCDQRVKVVARPLQITYDAPTILALINAFQTPGDVTLSKFEDAASTKISNFKERSATGMQYMIDKKAVLDVDILLMPNILVVPHKGVYDAGNVSLLVVSMGQVHLSSQPRRESNKLQHLFSAGEDKDEILKTVMENAYDRFTVAVDDVQMLVVRAGEPWQNALAEANSTEMHVLRPVSLKVTAALCVVDNDPRLPNIKVDIDLPAILVNVSEDRIFLAIKVATSIPLPEQKEPASRLTQTNSRSSMSISNFINKEVKKIGPSASGSSASKDPLLDEIIQYTSLDVNFSLGEINFVLFQSSRKCETSPDVSIEFLTPDGDVLPSQLTENIQEPIEELPPTPPQQILSIDIRRLEAHFVSKTYESVATVKLGDINLRQYDCQDSDMDVLDVIYTPKQENSSNYLFTVSCTIADKSSPEFSTKYNSTEQLVVANFEVLQIVLHQECLQRIMEVVNNFQRNLDLVLSSTRPRDRMGSIGGGDGIKRTLNVILEDTEEIMTTDQMKRRKKTRRTHVVETVKVRVIANLDQVGLVLTGRKRPIAEMNVKKFVSSLIIKSSYTEVNIGLKDIQVLDLNPYTIHKNILSIVGKDAFNCQIVIYNKEETQDYNSDDMKITVDIGCMKIIFLNWFVAGVMNFLNNFTAAQATISQAGAAAAESARQKAMDAYETATRMKLNIRIKAPIIIVPIGSQDRNALLLDLGLLELTNNTVEVAVAEEERLAVIDEIKLQICDVKISKIVLLDGNESTVDEVDAEVGFLSKFNMMNPMSCTLSITRNLSYTWYRDVPELNLSGRLKSIELTLFADDYALVMLVLNRNLNEGLEEFPPSEEAPQEAQVRPERRNSRAGRLSRTVQVSPIREKIHESIKFNFQFDGVVINLMEGEGAGLARFGIYFLSVKGTKLDNGTLSTSVVLCNIQMDDMRSNSKSQIRQYLSRKDWVQPKLDTDEIIDACYNERNFMVDVTAIIKEDDTFAEVRVRGFDLIVCIDFLLKLTTFLTLPPEENPRESVYIKPAPVSETARDTKHSIRSSAILAAQELVPVESSSHEVPNRKMNLILHIDEPDIILVENLEDLNTSCIIFNAQVHLNYRSINDKQIVNGQIDALKMYMCAFLPERREMTRHYILHPCVISLQGSTPEEEGMHISLKLSDIIINVSPATIELLNKAMLSVSSGTMTKCAIAEESRNYSNLWHQHHFHSRTYWFTKVEQGVDALEAEQRSVSTDNEKQKTEKCVIEIPSITLVIESGVGYYTKPLISLDTRITAVFNNWSRSLTAHGSLTLNMNYYNQALAEWEPIIELNEVIGRNGVREYTPWELKFEMGMEKVQSELEDDAEQQAMHMNIHSAETLEITLSKTCLGLLSELAEAFSQAIDQNGLTKPDIVAPYVLENDTGFDVNLNLRKGIFTLHEVHRGGTPVGANSTLLMVAQSEEVDPSVIKTCTISTGGRAYLQTKDLSTLSEEDSEDYTLYVTIGDINKEIALPVSKSDTRFFNLMRSTSHEPWGIISEVKQEYGTTKVNIHGVVSVHNHFTTGLNIYRRNPAPTAQCFEDIFVGRVRPGEVFHVPLHAIYAESKDLFFSMRGYRRSVQGISWASNPSDLNYSHQLHCDPTNTFEPLIMNARRSKSEVYFENTNKYTLLSAFYTIHLRPPLYLRNSLPINIQVSVAGCSVRKEDGLDAQSSQRFVDRGYRKEDFLDYGEKPVNSGDVLHLPTVRLASKGKESKSFLVVRLVQYLEKDWSCATEIWDYTDDVITWTFSSYDSEMKVDMDLYVKTENRHGSLMLTLFSPFWMINKTGMMLTYKSETTSVEVLYHPPEYSGPILFTFRDKLFFDKKKASIRIDNGQWSEKIPLDVAGSVGEVICFANNQKYPVGVHNHLTQNSLTKQITFIPFYIVCNKCHFDIELQEQSRPADPWLHLEPNEMEPLWPRNDTKNNLVVRVDGKITPAFDFTEVICTLLKLEDSKYGGINVDVQTTEGGVYITFTDYKPADAPGLLINHTGKQIVYHEKGTKNEHILNAKSTIMYAWDDPTGPKMLVFGTNKEETDLKRDGIGEVIMQDGGKVLWVSFLDGLQRVLLFTENESIANRTESTASLQSITQSIDLRIHGIGLSVINNETGLDILYLGVTSSGIIWESKKVTKNRFKELTINENALLEIEYQKYLVHKSVNDVQTYKLDNKFPIDFDLMILKKTVERNLRRSFYPAIWLSRKSSPFQSQLHVKINRIQVDNQFLDPIFPVVLAPIPPPKSVASTTSLKPFIECSMVQRIMPNSTVRQFKYARILIQEFLFKVDLNFLTAIAEMFAKEVSDEAAAKQFRQDVESIELPLSAFFEEHSLEEQKSFYDNLHLGPLKIHVSFSMAGSDTKALPGFLGSLVQGVGVTLTDVNDVVFRLAFFEREYQFFSQKQLINEITSHYTGQALKQLYVLVLGLDVLGNPYGLVVGLKKGVEDLFYEPFQGAIQGPGEFAEGLVLGVKSLFGHTVGGAAGAVSKITGAMGKGLAALTFDEDYQKKRRQGIQNKPKNFHEGLARSSKGLVMGFVDGVTGVVTKPVTGARDNGVEGFFKGLGKGAIGLVARPTAGVVDFASGSFEAVKRAADASEDVKRMRPPRFQHYDFVLRPYCLMEATGNKIMKETDKGKFATTDNFIHCEEIIQKSEYLVVTNYRVMYVQRNEMFGVWTSLWSYLWNEISSVAATARGVQFTVKTDGKKVLGLFSSKESPRKLVLVADERKRDALVDIIESQRSDPNPLRATIAYPAHN

Target ORF information:

RefSeq Version	NM_001273852.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 13, transcript variant B (Vps13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273852.1

ORF Insert Sequence:

ATGGTCTTCG AGGCGGTGGT GGCCGATGTG CTGAACAAGG TGCTCGGCGA CTACATCGAG 
AACTTGGACC GGAACCAGCT GAAGATTGGC ATTTGGGGAG GCGATGTCGT CTTGCAGAAC 
CTCAAGATAC GGGAGAATGC CCTGGACGAG TTGGACCTGC CCGTACAGCT TATCTATGGT 
TACTTGGGCA AACTAGTGCT TAAGATCCCC TGGAAGAATT TGTACAGCCA GCCGGTAATT 
GTCAACATTG AGGATCTTTA TGTCTTAGTT TCACCCAACA ACAATGTCCA GTACAACGCC 
GAAAAGGAGG CCAAATATGA GATGGACCTC AAGAAGGCGG CGCTTGATGC CCTGGAGGCA 
GCTCGCAAAA AGGAGTTGGA AATGGATCAG CCCAAAGCAG ATGCAGGATT TGCCGAAAAG 
CTAACGGCGC AGATCGTTAA CAATCTGCAG GTGCAGATAA CCAACGTTCA TCTGCGATAC 
GAGGATACCA CAACGACGGG TAGTCCCTTC TCCTTCGGCA TATCCCTGCA TGAGCTCGAA 
CTTTACACCA CCGATTGCGA CTGGGAGAAG TGCTACATGG CCCAGCAGGC GTCGCAGGTC 
TTCAAAATAG CGAATCTCTC ATGTCTTTCC GCCTATTTGA ACTGTGGTGG ACAACTGTAC 
GCGAACAACA AGAGCGACCT TTCACAGCAG TTCAAGACGA ATATTGCTTG CAAGGAAACA 
AAACCCAACT ACAACTATGT GCTGGGTCCT ATCTCTTGCA ATGCCAAGCT AAAGCTAAAC 
ATGAACCCGG AGCTCGATGA TCCGCCATTT GAAAAACCTA AAATCGATCT TACGCTGGAA 
ATGGAAAAGC TTAATGTCGG CTTGACAAAC ACACAGTTCG ACAATCTCAT GAAGCTTGGA 
GACGCTATGA ACCGCCAGCA GTTGGGCATA CCGTACCGGA AATATCGTCC CTACAATATA 
CCATACAAAG GCCACGCCAG GGACTGGTGG CACTTTGCAA TAACTTCTAT TCTAGAGGAG 
GAAGTAAGGA AACCCAGAGA GAGCTGGACT TGGGGACACA TCAAGACCCA CAGAGAACGC 
TGCAACACTT ATGCACAGAA ATACAAGGAG CAGTGCTTAA GCAAAAAACC GTCAGCCGTG 
CTTACGGAAA CCTGCCGTTT GCTAGAAACG GAGCTGGACG TTTTTAACTT GCTGCTAATC 
CGGCAGAGGG TCAACATCGA GATAGCCAAG CAACGAGAGG CAGTGCCTGA ACAAAAGTCG 
GGCTGGTTCA GTGGTTGGGG ATGGGGCGGC GGAGCCAAGA AGGATGACCA AACCACCAGT 
CAAAAACTTG TTGAGAAGTT TGAAGCGGCA ATGACCAGTG AAGAGAAAGA GAAGATGTAC 
AGAGCTATTG GTTACCAAGA GAACGCCAAG CCCACAGACC TTCCAGAGAG CTACGAGGCC 
ATCAGGATGA ACTTTAAACT GATAGCCTTA GAGGTCGGCC TTTACAAGGA TGAGCGTAAT 
AGTTCCGCGG CAACGAAGGA CTTCCATGAG CTGCCGTCGC TGGTCCTTCT TAACTTTTCT 
ATGGCCACCG CGTTGATCAC CCAACGGCCA GCTGCCGAAG CCATCAGCAT TATTGCTGGC 
ATGAGAGAAA TCAAGGTAAC TGGTCTCACG CGCAACGATT ACACGCCTTT ATTGGTTGAG 
TCAAAAATCA CCGACGAGTT CAACCTGCTG GAAGTGTTTT TCGAGACAAA CCCACTGGAC 
AAGTTGTGCG ACCAGCGAGT GAAGGTTGTG GCGCGCCCGT TGCAGATAAC GTATGATGCG 
CCAACCATAT TGGCCCTGAT AAACGCATTC CAGACGCCTG GCGATGTCAC GCTCTCCAAG 
TTTGAGGATG CTGCCAGCAC GAAAATCTCG AACTTCAAGG AGCGTTCAGC CACTGGAATG 
CAGTACATGA TCGACAAGAA GGCAGTGCTG GATGTGGACA TCCTGTTAAT GCCAAACATC 
CTGGTCGTAC CACATAAAGG AGTCTACGAT GCGGGCAACG TATCCCTGCT AGTAGTTAGC 
ATGGGCCAGG TGCACCTCTC CAGCCAACCG CGTAGGGAGT CGAACAAGCT GCAGCATCTT 
TTCTCAGCCG GCGAGGATAA GGATGAGATC CTCAAGACGG TAATGGAAAA TGCTTATGAC 
AGGTTTACAG TCGCAGTTGA CGATGTGCAG ATGCTGGTCG TGCGAGCTGG AGAGCCGTGG 
CAAAATGCGC TAGCGGAGGC AAACTCAACG GAGATGCACG TTTTGCGCCC CGTCTCCCTA 
AAAGTAACTG CCGCACTATG TGTGGTTGAC AATGATCCGC GTCTTCCAAA TATTAAAGTG 
GACATCGACT TGCCTGCGAT CCTAGTTAAT GTCTCAGAGG ATCGCATTTT CCTCGCCATA 
AAGGTGGCAA CAAGTATTCC CCTGCCGGAG CAAAAGGAAC CGGCTAGTAG ACTCACACAG 
ACGAACTCGC GCAGCTCAAT GTCCATATCC AATTTCATCA ACAAGGAAGT GAAAAAGATT 
GGGCCTTCGG CATCGGGGAG CTCTGCCAGT AAGGATCCGT TGCTGGACGA GATCATTCAG 
TACACCAGTC TCGATGTAAA TTTTTCTCTT GGAGAAATCA ACTTCGTTTT GTTCCAGTCC 
AGTAGAAAAT GCGAGACAAG CCCCGATGTT TCTATTGAAT TTCTGACTCC TGATGGTGAT 
GTCCTTCCTT CTCAGTTGAC TGAGAATATC CAGGAGCCTA TTGAAGAGCT ACCCCCAACG 
CCACCTCAGC AGATTCTCTC AATCGACATT CGACGCCTGG AGGCCCACTT TGTGTCCAAA 
ACCTATGAAT CGGTGGCCAC AGTCAAACTT GGAGATATCA ACCTGCGACA GTATGACTGC 
CAGGACAGTG ACATGGATGT ACTAGATGTT ATCTATACGC CGAAACAGGA GAATAGTTCC 
AATTACCTGT TCACCGTCTC GTGCACAATT GCAGACAAGT CGTCGCCAGA GTTCAGCACC 
AAGTACAACT CCACTGAACA GTTGGTCGTG GCTAACTTCG AGGTACTTCA AATCGTGCTG 
CACCAGGAAT GCCTACAAAG GATTATGGAG GTCGTAAACA ATTTCCAAAG AAACCTAGAT 
CTTGTACTAT CAAGTACCCG ACCACGGGAT CGGATGGGCT CCATTGGGGG AGGAGATGGC 
ATTAAACGTA CGCTTAATGT TATTCTTGAG GACACAGAGG AAATAATGAC CACCGACCAG 
ATGAAGCGGC GAAAGAAGAC GCGCCGCACT CACGTCGTAG AGACGGTGAA AGTTCGTGTT 
ATCGCTAATT TGGATCAAGT GGGTCTGGTG CTGACCGGCC GAAAGCGGCC GATAGCAGAG 
ATGAATGTGA AGAAATTCGT GTCCAGTCTG ATCATAAAGT CGTCCTACAC GGAAGTCAAC 
ATTGGCCTTA AAGACATTCA GGTGTTGGAC CTAAATCCTT ACACTATTCA CAAGAATATT 
CTGAGTATTG TTGGCAAGGA CGCTTTCAAC TGCCAGATTG TGATCTACAA CAAGGAGGAG 
ACCCAAGACT ACAACTCTGA TGATATGAAA ATAACCGTGG ACATTGGCTG CATGAAGATC 
ATCTTCCTCA ATTGGTTTGT GGCAGGTGTT ATGAACTTCT TAAACAACTT CACAGCGGCC 
CAAGCAACCA TATCGCAGGC TGGTGCTGCG GCTGCCGAGT CCGCTCGCCA GAAGGCAATG 
GATGCCTATG AAACGGCCAC TCGTATGAAA CTAAATATCC GCATCAAGGC TCCCATTATC 
ATTGTGCCAA TTGGGTCACA GGACAGGAAT GCCTTGCTGC TCGATTTGGG CTTACTGGAG 
CTAACGAATA ACACGGTGGA GGTGGCGGTG GCCGAAGAGG AACGGCTAGC TGTTATAGAC 
GAGATCAAGT TGCAAATATG CGATGTTAAG ATTTCCAAAA TTGTCTTATT AGATGGCAAC 
GAGTCAACGG TTGATGAAGT GGACGCGGAA GTGGGTTTCC TCTCTAAATT CAATATGATG 
AATCCCATGA GTTGCACCTT ATCAATCACG CGCAATTTGT CATACACATG GTACCGGGAT 
GTGCCCGAAC TAAATCTTTC CGGTCGATTA AAGTCCATTG AGCTCACTCT ATTCGCGGAT 
GATTATGCTT TGGTAATGCT TGTTCTCAAT CGCAATTTAA ACGAGGGCTT GGAAGAGTTC 
CCTCCAAGCG AGGAGGCGCC GCAGGAAGCA CAAGTTAGAC CCGAAAGACG CAACTCTCGT 
GCGGGACGTT TATCTCGTAC CGTTCAAGTT TCTCCGATAA GGGAAAAGAT TCACGAATCA 
ATCAAGTTTA ACTTTCAGTT TGACGGTGTG GTCATAAATT TAATGGAGGG TGAAGGAGCA 
GGACTCGCCA GGTTTGGAAT ATATTTCCTC TCCGTTAAGG GCACCAAGTT GGACAACGGC 
ACACTGAGCA CTTCTGTTGT ATTGTGCAAT ATTCAAATGG ATGACATGCG AAGTAATTCC 
AAAAGCCAGA TCCGACAGTA CTTGAGTCGC AAGGATTGGG TTCAGCCTAA GTTGGATACA 
GACGAGATCA TCGATGCCTG TTACAACGAG CGCAACTTCA TGGTCGATGT GACGGCTATA 
ATAAAAGAAG ATGACACGTT CGCTGAGGTC CGTGTGCGTG GCTTCGATCT GATCGTCTGC 
ATCGACTTCC TGCTGAAACT GACTACGTTT CTTACCTTGC CGCCTGAGGA GAATCCGCGC 
GAGTCTGTTT ATATAAAGCC AGCGCCTGTT TCGGAAACTG CTCGGGATAC CAAGCACTCT 
ATTCGCTCCT CCGCCATATT AGCCGCCCAA GAGCTGGTTC CTGTCGAGTC ATCCAGTCAC 
GAAGTTCCAA ACCGAAAAAT GAATTTAATA CTGCATATTG ACGAGCCAGA TATAATATTA 
GTGGAAAATC TGGAAGATCT TAATACCAGT TGTATTATTT TCAATGCTCA AGTGCATTTG 
AATTACCGCA GCATAAACGA CAAACAGATT GTAAACGGAC AGATCGATGC TCTGAAGATG 
TACATGTGTG CTTTCCTACC AGAGCGGCGA GAGATGACGC GTCATTATAT CCTTCACCCG 
TGCGTTATTA GCCTGCAAGG ATCAACTCCC GAAGAAGAGG GCATGCACAT TAGCCTAAAG 
CTCAGTGACA TTATCATCAA CGTGTCGCCG GCCACCATCG AGCTGCTAAA CAAGGCAATG 
CTGAGCGTCT CCAGTGGCAC CATGACCAAG TGCGCTATTG CCGAGGAGTC GCGGAACTAT 
TCGAATCTGT GGCATCAGCA CCACTTCCAT AGCCGCACGT ATTGGTTCAC CAAGGTGGAA 
CAGGGCGTCG ATGCCCTGGA GGCTGAGCAG AGAAGCGTCA GCACTGACAA CGAAAAGCAG 
AAGACAGAAA AGTGTGTAAT TGAAATTCCC AGCATTACGC TCGTGATTGA GTCCGGCGTA 
GGCTACTACA CGAAGCCACT TATTTCGCTA GACACCCGCA TTACTGCCGT TTTCAACAAT 
TGGAGCCGCA GTCTCACCGC TCATGGCTCG TTGACACTAA ATATGAATTA CTATAACCAA 
GCCCTTGCCG AATGGGAGCC TATTATTGAG CTGAACGAGG TCATTGGTCG CAACGGCGTT 
CGTGAATACA CGCCATGGGA GCTTAAGTTC GAAATGGGCA TGGAGAAGGT GCAGAGCGAA 
TTGGAAGACG ATGCTGAACA GCAGGCGATG CACATGAACA TCCACTCGGC AGAGACGCTG 
GAGATTACGT TGAGCAAGAC GTGCCTGGGT CTATTGAGCG AACTGGCCGA GGCCTTCTCG 
CAGGCTATTG ACCAGAACGG TCTTACCAAG CCAGACATCG TGGCTCCTTA CGTGCTGGAA 
AACGACACTG GCTTCGATGT TAACCTGAAT CTGCGGAAGG GCATCTTTAC ACTGCACGAA 
GTGCACCGCG GCGGCACACC TGTGGGTGCA AATAGTACCC TTCTTATGGT GGCCCAATCT 
GAAGAGGTCG ATCCCAGCGT CATAAAAACC TGCACCATTT CGACGGGCGG AAGGGCATAT 
CTGCAGACTA AGGATCTGAG CACGTTATCA GAAGAGGATT CCGAGGACTA CACTCTTTAT 
GTGACGATTG GAGATATCAA CAAGGAGATC GCACTGCCCG TTTCCAAGTC GGACACGCGT 
TTCTTCAACC TGATGCGCAG TACCTCTCAC GAGCCTTGGG GCATTATTTC TGAAGTAAAG 
CAGGAGTACG GCACAACCAA AGTCAATATC CATGGTGTTG TTAGTGTTCA TAATCACTTT 
ACGACCGGCT TGAATATTTA TCGACGCAAT CCTGCCCCTA CTGCACAATG TTTCGAGGAT 
ATTTTCGTCG GACGTGTGCG TCCCGGCGAA GTGTTCCATG TACCACTGCA CGCCATATAC 
GCGGAATCCA AGGATTTATT CTTTTCCATG CGCGGCTACA GAAGATCAGT GCAGGGCATT 
TCGTGGGCTT CCAATCCCTC GGACCTTAAC TATTCGCATC AGTTGCATTG TGATCCGACC 
AATACGTTCG AACCGCTTAT CATGAACGCT CGAAGATCAA AATCGGAGGT GTACTTCGAA 
AACACCAACA AGTATACATT GCTAAGCGCC TTCTACACGA TTCATCTGCG CCCTCCACTC 
TATCTGCGCA ACTCTCTGCC CATTAATATC CAAGTGTCAG TTGCCGGCTG CTCTGTCCGA 
AAAGAGGATG GCCTGGATGC ACAGTCGTCG CAGCGCTTTG TTGACAGAGG CTATCGTAAG 
GAAGACTTTC TGGACTATGG AGAAAAGCCT GTAAATTCCG GCGACGTGCT CCACCTGCCG 
ACCGTTCGAT TGGCCTCGAA AGGCAAGGAA TCGAAGAGTT TCCTAGTTGT GCGGCTGGTT 
CAATATCTGG AGAAAGACTG GTCCTGCGCT ACCGAGATCT GGGATTACAC TGATGATGTT 
ATTACCTGGA CGTTTTCATC ATATGACTCG GAAATGAAGG TGGACATGGA TCTTTATGTG 
AAAACAGAGA ACCGACATGG CAGCTTGATG CTTACGCTTT TTAGCCCTTT TTGGATGATC 
AATAAGACCG GCATGATGCT CACTTACAAA TCGGAAACGA CCTCCGTGGA AGTTCTCTAC 
CATCCTCCTG AGTATTCTGG TCCCATTTTG TTCACTTTCC GGGATAAGCT ATTCTTTGAC 
AAGAAAAAAG CTTCAATTCG CATAGACAAT GGTCAATGGA GTGAAAAGAT TCCATTGGAC 
GTGGCGGGTT CGGTTGGCGA AGTGATCTGC TTTGCGAACA ATCAGAAATA CCCTGTCGGT 
GTTCACAACC ACTTGACACA GAACTCTTTG ACCAAGCAGA TAACGTTCAT ACCCTTTTAT 
ATTGTGTGCA ACAAGTGTCA CTTTGATATA GAACTGCAGG AGCAGAGTCG TCCAGCCGAT 
CCATGGCTCC ATCTCGAGCC AAATGAGATG GAACCTCTCT GGCCGAGAAA CGACACAAAG 
AACAATTTGG TGGTGCGCGT GGATGGCAAG ATTACACCGG CCTTCGACTT TACGGAGGTG 
ATCTGTACGC TCCTGAAACT GGAGGACAGC AAGTACGGCG GTATCAATGT GGACGTTCAG 
ACAACGGAAG GCGGCGTCTA TATCACCTTT ACGGACTATA AGCCAGCCGA TGCGCCTGGC 
CTGCTCATCA ATCACACTGG CAAGCAGATT GTTTACCACG AGAAGGGAAC CAAAAACGAG 
CATATCCTAA ATGCAAAGAG TACTATAATG TATGCCTGGG ACGATCCTAC AGGTCCAAAA 
ATGCTTGTGT TCGGCACAAA CAAGGAAGAG ACGGATCTGA AACGAGACGG AATTGGCGAA 
GTGATCATGC AAGACGGCGG AAAGGTTCTA TGGGTATCAT TCCTCGACGG CCTGCAACGT 
GTGCTGCTCT TTACGGAAAA CGAATCGATC GCCAATCGTA CGGAAAGCAC GGCCTCGTTA 
CAGTCTATTA CCCAGAGCAT TGATCTGCGT ATTCACGGCA TTGGCTTGTC AGTGATCAAC 
AACGAGACGG GCCTTGACAT ACTTTATCTG GGAGTTACCA GCTCCGGCAT TATATGGGAG 
TCAAAGAAAG TGACCAAGAA TCGCTTTAAG GAGTTAACCA TTAACGAAAA CGCGCTTTTG 
GAAATTGAGT ATCAAAAATA TCTAGTTCAC AAGAGTGTCA ACGATGTGCA GACCTACAAG 
CTAGATAACA AGTTCCCGAT CGACTTCGAC CTTATGATCC TCAAGAAGAC GGTGGAGAGA 
AACTTAAGAC GCAGTTTCTA TCCAGCGATT TGGTTGTCCC GCAAGTCGTC GCCATTCCAA 
AGCCAGCTGC ATGTGAAAAT TAATCGTATA CAGGTGGATA ATCAGTTCCT GGATCCAATA 
TTCCCTGTGG TGTTGGCGCC AATTCCACCC CCGAAGAGTG TGGCAAGTAC CACCAGTCTT 
AAACCATTTA TCGAGTGCAG CATGGTGCAG CGCATCATGC CGAACTCAAC TGTGCGTCAG 
TTCAAGTACG CCAGGATACT GATCCAAGAG TTCCTCTTCA AAGTCGATTT GAACTTCCTT 
ACTGCCATCG CCGAAATGTT TGCCAAGGAG GTCTCGGACG AGGCAGCAGC TAAGCAGTTC 
CGTCAGGATG TTGAATCAAT TGAGCTCCCG CTTTCAGCGT TCTTTGAAGA GCACTCGTTG 
GAGGAGCAGA AGAGCTTTTA CGATAACCTG CATTTGGGTC CGCTCAAGAT CCATGTCAGC 
TTCTCGATGG CCGGGTCGGA CACAAAGGCG TTGCCCGGTT TCTTGGGCTC ATTGGTCCAG 
GGCGTGGGTG TGACTCTAAC GGACGTAAAC GATGTTGTTT TCCGTCTGGC CTTCTTCGAG 
CGCGAGTATC AATTCTTCTC CCAAAAGCAG CTAATCAACG AGATCACCTC ACACTACACT 
GGCCAAGCGT TAAAGCAGCT GTATGTGCTC GTCCTCGGAC TTGATGTCCT GGGCAATCCC 
TATGGCCTAG TGGTTGGTCT TAAGAAGGGC GTCGAAGACT TGTTCTATGA ACCGTTCCAG 
GGTGCCATCC AAGGTCCTGG AGAATTCGCC GAGGGTTTGG TCCTGGGCGT AAAGTCTCTT 
TTCGGCCACA CTGTGGGTGG TGCTGCGGGA GCGGTTTCGA AAATAACTGG TGCTATGGGA 
AAGGGTTTGG CTGCGCTGAC CTTCGACGAG GACTATCAAA AGAAACGACG ACAGGGTATT 
CAAAACAAAC CGAAGAACTT CCACGAAGGA TTGGCCCGCA GCAGCAAGGG TCTAGTGATG 
GGCTTTGTGG ACGGTGTCAC TGGCGTTGTA ACCAAACCAG TGACTGGGGC TCGCGATAAC 
GGGGTCGAGG GCTTCTTCAA GGGCCTGGGT AAGGGTGCAA TTGGCCTGGT TGCTCGCCCC 
ACCGCTGGCG TGGTTGACTT TGCCAGTGGC AGCTTCGAGG CTGTGAAACG GGCGGCGGAT 
GCAAGCGAGG ATGTGAAGCG TATGCGACCG CCGCGTTTCC AGCACTACGA CTTCGTGTTG 
AGACCCTATT GCTTGATGGA GGCGACAGGG AATAAAATAA TGAAGGAAAC CGACAAGGGT 
AAATTTGCCA CCACAGATAA CTTCATTCAC TGTGAGGAGA TTATCCAAAA GTCCGAGTAC 
TTGGTGGTGA CCAATTACCG GGTGATGTAC GTGCAACGTA ACGAGATGTT CGGCGTTTGG 
ACGTCTCTTT GGTCCTACCT ATGGAACGAG ATTAGCTCCG TGGCTGCAAC GGCTCGCGGT 
GTCCAGTTCA CAGTAAAGAC GGATGGCAAG AAGGTACTTG GTCTGTTTAG CAGCAAGGAG 
TCACCTCGAA AGTTAGTGCT CGTGGCAGAT GAGCGCAAAC GTGACGCTCT TGTGGACATT 
ATAGAATCTC AGCGCTCCGA TCCCAATCCG TTAAGGGCCA CCATCGCCTA TCCGGCTCAT 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps13 ( NM_136455.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610299.2 Drosophila melanogaster vacuolar protein sorting 13, transcript variant A (Vps13), mRNA.

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CloneID	OFa09160
Clone ID Related Accession (Same CDS sequence)	NM_001273852.1 , NM_136455.3
Accession Version	NM_136455.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 13, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136455.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCTTCG AGGCGGTGGT GGCCGATGTG CTGAACAAGG TGCTCGGCGA CTACATCGAG 
AACTTGGACC GGAACCAGCT GAAGATTGGC ATTTGGGGAG GCGATGTCGT CTTGCAGAAC 
CTCAAGATAC GGGAGAATGC CCTGGACGAG TTGGACCTGC CCGTACAGCT TATCTATGGT 
TACTTGGGCA AACTAGTGCT TAAGATCCCC TGGAAGAATT TGTACAGCCA GCCGGTAATT 
GTCAACATTG AGGATCTTTA TGTCTTAGTT TCACCCAACA ACAATGTCCA GTACAACGCC 
GAAAAGGAGG CCAAATATGA GATGGACCTC AAGAAGGCGG CGCTTGATGC CCTGGAGGCA 
GCTCGCAAAA AGGAGTTGGA AATGGATCAG CCCAAAGCAG ATGCAGGATT TGCCGAAAAG 
CTAACGGCGC AGATCGTTAA CAATCTGCAG GTGCAGATAA CCAACGTTCA TCTGCGATAC 
GAGGATACCA CAACGACGGG TAGTCCCTTC TCCTTCGGCA TATCCCTGCA TGAGCTCGAA 
CTTTACACCA CCGATTGCGA CTGGGAGAAG TGCTACATGG CCCAGCAGGC GTCGCAGGTC 
TTCAAAATAG CGAATCTCTC ATGTCTTTCC GCCTATTTGA ACTGTGGTGG ACAACTGTAC 
GCGAACAACA AGAGCGACCT TTCACAGCAG TTCAAGACGA ATATTGCTTG CAAGGAAACA 
AAACCCAACT ACAACTATGT GCTGGGTCCT ATCTCTTGCA ATGCCAAGCT AAAGCTAAAC 
ATGAACCCGG AGCTCGATGA TCCGCCATTT GAAAAACCTA AAATCGATCT TACGCTGGAA 
ATGGAAAAGC TTAATGTCGG CTTGACAAAC ACACAGTTCG ACAATCTCAT GAAGCTTGGA 
GACGCTATGA ACCGCCAGCA GTTGGGCATA CCGTACCGGA AATATCGTCC CTACAATATA 
CCATACAAAG GCCACGCCAG GGACTGGTGG CACTTTGCAA TAACTTCTAT TCTAGAGGAG 
GAAGTAAGGA AACCCAGAGA GAGCTGGACT TGGGGACACA TCAAGACCCA CAGAGAACGC 
TGCAACACTT ATGCACAGAA ATACAAGGAG CAGTGCTTAA GCAAAAAACC GTCAGCCGTG 
CTTACGGAAA CCTGCCGTTT GCTAGAAACG GAGCTGGACG TTTTTAACTT GCTGCTAATC 
CGGCAGAGGG TCAACATCGA GATAGCCAAG CAACGAGAGG CAGTGCCTGA ACAAAAGTCG 
GGCTGGTTCA GTGGTTGGGG ATGGGGCGGC GGAGCCAAGA AGGATGACCA AACCACCAGT 
CAAAAACTTG TTGAGAAGTT TGAAGCGGCA ATGACCAGTG AAGAGAAAGA GAAGATGTAC 
AGAGCTATTG GTTACCAAGA GAACGCCAAG CCCACAGACC TTCCAGAGAG CTACGAGGCC 
ATCAGGATGA ACTTTAAACT GATAGCCTTA GAGGTCGGCC TTTACAAGGA TGAGCGTAAT 
AGTTCCGCGG CAACGAAGGA CTTCCATGAG CTGCCGTCGC TGGTCCTTCT TAACTTTTCT 
ATGGCCACCG CGTTGATCAC CCAACGGCCA GCTGCCGAAG CCATCAGCAT TATTGCTGGC 
ATGAGAGAAA TCAAGGTAAC TGGTCTCACG CGCAACGATT ACACGCCTTT ATTGGTTGAG 
TCAAAAATCA CCGACGAGTT CAACCTGCTG GAAGTGTTTT TCGAGACAAA CCCACTGGAC 
AAGTTGTGCG ACCAGCGAGT GAAGGTTGTG GCGCGCCCGT TGCAGATAAC GTATGATGCG 
CCAACCATAT TGGCCCTGAT AAACGCATTC CAGACGCCTG GCGATGTCAC GCTCTCCAAG 
TTTGAGGATG CTGCCAGCAC GAAAATCTCG AACTTCAAGG AGCGTTCAGC CACTGGAATG 
CAGTACATGA TCGACAAGAA GGCAGTGCTG GATGTGGACA TCCTGTTAAT GCCAAACATC 
CTGGTCGTAC CACATAAAGG AGTCTACGAT GCGGGCAACG TATCCCTGCT AGTAGTTAGC 
ATGGGCCAGG TGCACCTCTC CAGCCAACCG CGTAGGGAGT CGAACAAGCT GCAGCATCTT 
TTCTCAGCCG GCGAGGATAA GGATGAGATC CTCAAGACGG TAATGGAAAA TGCTTATGAC 
AGGTTTACAG TCGCAGTTGA CGATGTGCAG ATGCTGGTCG TGCGAGCTGG AGAGCCGTGG 
CAAAATGCGC TAGCGGAGGC AAACTCAACG GAGATGCACG TTTTGCGCCC CGTCTCCCTA 
AAAGTAACTG CCGCACTATG TGTGGTTGAC AATGATCCGC GTCTTCCAAA TATTAAAGTG 
GACATCGACT TGCCTGCGAT CCTAGTTAAT GTCTCAGAGG ATCGCATTTT CCTCGCCATA 
AAGGTGGCAA CAAGTATTCC CCTGCCGGAG CAAAAGGAAC CGGCTAGTAG ACTCACACAG 
ACGAACTCGC GCAGCTCAAT GTCCATATCC AATTTCATCA ACAAGGAAGT GAAAAAGATT 
GGGCCTTCGG CATCGGGGAG CTCTGCCAGT AAGGATCCGT TGCTGGACGA GATCATTCAG 
TACACCAGTC TCGATGTAAA TTTTTCTCTT GGAGAAATCA ACTTCGTTTT GTTCCAGTCC 
AGTAGAAAAT GCGAGACAAG CCCCGATGTT TCTATTGAAT TTCTGACTCC TGATGGTGAT 
GTCCTTCCTT CTCAGTTGAC TGAGAATATC CAGGAGCCTA TTGAAGAGCT ACCCCCAACG 
CCACCTCAGC AGATTCTCTC AATCGACATT CGACGCCTGG AGGCCCACTT TGTGTCCAAA 
ACCTATGAAT CGGTGGCCAC AGTCAAACTT GGAGATATCA ACCTGCGACA GTATGACTGC 
CAGGACAGTG ACATGGATGT ACTAGATGTT ATCTATACGC CGAAACAGGA GAATAGTTCC 
AATTACCTGT TCACCGTCTC GTGCACAATT GCAGACAAGT CGTCGCCAGA GTTCAGCACC 
AAGTACAACT CCACTGAACA GTTGGTCGTG GCTAACTTCG AGGTACTTCA AATCGTGCTG 
CACCAGGAAT GCCTACAAAG GATTATGGAG GTCGTAAACA ATTTCCAAAG AAACCTAGAT 
CTTGTACTAT CAAGTACCCG ACCACGGGAT CGGATGGGCT CCATTGGGGG AGGAGATGGC 
ATTAAACGTA CGCTTAATGT TATTCTTGAG GACACAGAGG AAATAATGAC CACCGACCAG 
ATGAAGCGGC GAAAGAAGAC GCGCCGCACT CACGTCGTAG AGACGGTGAA AGTTCGTGTT 
ATCGCTAATT TGGATCAAGT GGGTCTGGTG CTGACCGGCC GAAAGCGGCC GATAGCAGAG 
ATGAATGTGA AGAAATTCGT GTCCAGTCTG ATCATAAAGT CGTCCTACAC GGAAGTCAAC 
ATTGGCCTTA AAGACATTCA GGTGTTGGAC CTAAATCCTT ACACTATTCA CAAGAATATT 
CTGAGTATTG TTGGCAAGGA CGCTTTCAAC TGCCAGATTG TGATCTACAA CAAGGAGGAG 
ACCCAAGACT ACAACTCTGA TGATATGAAA ATAACCGTGG ACATTGGCTG CATGAAGATC 
ATCTTCCTCA ATTGGTTTGT GGCAGGTGTT ATGAACTTCT TAAACAACTT CACAGCGGCC 
CAAGCAACCA TATCGCAGGC TGGTGCTGCG GCTGCCGAGT CCGCTCGCCA GAAGGCAATG 
GATGCCTATG AAACGGCCAC TCGTATGAAA CTAAATATCC GCATCAAGGC TCCCATTATC 
ATTGTGCCAA TTGGGTCACA GGACAGGAAT GCCTTGCTGC TCGATTTGGG CTTACTGGAG 
CTAACGAATA ACACGGTGGA GGTGGCGGTG GCCGAAGAGG AACGGCTAGC TGTTATAGAC 
GAGATCAAGT TGCAAATATG CGATGTTAAG ATTTCCAAAA TTGTCTTATT AGATGGCAAC 
GAGTCAACGG TTGATGAAGT GGACGCGGAA GTGGGTTTCC TCTCTAAATT CAATATGATG 
AATCCCATGA GTTGCACCTT ATCAATCACG CGCAATTTGT CATACACATG GTACCGGGAT 
GTGCCCGAAC TAAATCTTTC CGGTCGATTA AAGTCCATTG AGCTCACTCT ATTCGCGGAT 
GATTATGCTT TGGTAATGCT TGTTCTCAAT CGCAATTTAA ACGAGGGCTT GGAAGAGTTC 
CCTCCAAGCG AGGAGGCGCC GCAGGAAGCA CAAGTTAGAC CCGAAAGACG CAACTCTCGT 
GCGGGACGTT TATCTCGTAC CGTTCAAGTT TCTCCGATAA GGGAAAAGAT TCACGAATCA 
ATCAAGTTTA ACTTTCAGTT TGACGGTGTG GTCATAAATT TAATGGAGGG TGAAGGAGCA 
GGACTCGCCA GGTTTGGAAT ATATTTCCTC TCCGTTAAGG GCACCAAGTT GGACAACGGC 
ACACTGAGCA CTTCTGTTGT ATTGTGCAAT ATTCAAATGG ATGACATGCG AAGTAATTCC 
AAAAGCCAGA TCCGACAGTA CTTGAGTCGC AAGGATTGGG TTCAGCCTAA GTTGGATACA 
GACGAGATCA TCGATGCCTG TTACAACGAG CGCAACTTCA TGGTCGATGT GACGGCTATA 
ATAAAAGAAG ATGACACGTT CGCTGAGGTC CGTGTGCGTG GCTTCGATCT GATCGTCTGC 
ATCGACTTCC TGCTGAAACT GACTACGTTT CTTACCTTGC CGCCTGAGGA GAATCCGCGC 
GAGTCTGTTT ATATAAAGCC AGCGCCTGTT TCGGAAACTG CTCGGGATAC CAAGCACTCT 
ATTCGCTCCT CCGCCATATT AGCCGCCCAA GAGCTGGTTC CTGTCGAGTC ATCCAGTCAC 
GAAGTTCCAA ACCGAAAAAT GAATTTAATA CTGCATATTG ACGAGCCAGA TATAATATTA 
GTGGAAAATC TGGAAGATCT TAATACCAGT TGTATTATTT TCAATGCTCA AGTGCATTTG 
AATTACCGCA GCATAAACGA CAAACAGATT GTAAACGGAC AGATCGATGC TCTGAAGATG 
TACATGTGTG CTTTCCTACC AGAGCGGCGA GAGATGACGC GTCATTATAT CCTTCACCCG 
TGCGTTATTA GCCTGCAAGG ATCAACTCCC GAAGAAGAGG GCATGCACAT TAGCCTAAAG 
CTCAGTGACA TTATCATCAA CGTGTCGCCG GCCACCATCG AGCTGCTAAA CAAGGCAATG 
CTGAGCGTCT CCAGTGGCAC CATGACCAAG TGCGCTATTG CCGAGGAGTC GCGGAACTAT 
TCGAATCTGT GGCATCAGCA CCACTTCCAT AGCCGCACGT ATTGGTTCAC CAAGGTGGAA 
CAGGGCGTCG ATGCCCTGGA GGCTGAGCAG AGAAGCGTCA GCACTGACAA CGAAAAGCAG 
AAGACAGAAA AGTGTGTAAT TGAAATTCCC AGCATTACGC TCGTGATTGA GTCCGGCGTA 
GGCTACTACA CGAAGCCACT TATTTCGCTA GACACCCGCA TTACTGCCGT TTTCAACAAT 
TGGAGCCGCA GTCTCACCGC TCATGGCTCG TTGACACTAA ATATGAATTA CTATAACCAA 
GCCCTTGCCG AATGGGAGCC TATTATTGAG CTGAACGAGG TCATTGGTCG CAACGGCGTT 
CGTGAATACA CGCCATGGGA GCTTAAGTTC GAAATGGGCA TGGAGAAGGT GCAGAGCGAA 
TTGGAAGACG ATGCTGAACA GCAGGCGATG CACATGAACA TCCACTCGGC AGAGACGCTG 
GAGATTACGT TGAGCAAGAC GTGCCTGGGT CTATTGAGCG AACTGGCCGA GGCCTTCTCG 
CAGGCTATTG ACCAGAACGG TCTTACCAAG CCAGACATCG TGGCTCCTTA CGTGCTGGAA 
AACGACACTG GCTTCGATGT TAACCTGAAT CTGCGGAAGG GCATCTTTAC ACTGCACGAA 
GTGCACCGCG GCGGCACACC TGTGGGTGCA AATAGTACCC TTCTTATGGT GGCCCAATCT 
GAAGAGGTCG ATCCCAGCGT CATAAAAACC TGCACCATTT CGACGGGCGG AAGGGCATAT 
CTGCAGACTA AGGATCTGAG CACGTTATCA GAAGAGGATT CCGAGGACTA CACTCTTTAT 
GTGACGATTG GAGATATCAA CAAGGAGATC GCACTGCCCG TTTCCAAGTC GGACACGCGT 
TTCTTCAACC TGATGCGCAG TACCTCTCAC GAGCCTTGGG GCATTATTTC TGAAGTAAAG 
CAGGAGTACG GCACAACCAA AGTCAATATC CATGGTGTTG TTAGTGTTCA TAATCACTTT 
ACGACCGGCT TGAATATTTA TCGACGCAAT CCTGCCCCTA CTGCACAATG TTTCGAGGAT 
ATTTTCGTCG GACGTGTGCG TCCCGGCGAA GTGTTCCATG TACCACTGCA CGCCATATAC 
GCGGAATCCA AGGATTTATT CTTTTCCATG CGCGGCTACA GAAGATCAGT GCAGGGCATT 
TCGTGGGCTT CCAATCCCTC GGACCTTAAC TATTCGCATC AGTTGCATTG TGATCCGACC 
AATACGTTCG AACCGCTTAT CATGAACGCT CGAAGATCAA AATCGGAGGT GTACTTCGAA 
AACACCAACA AGTATACATT GCTAAGCGCC TTCTACACGA TTCATCTGCG CCCTCCACTC 
TATCTGCGCA ACTCTCTGCC CATTAATATC CAAGTGTCAG TTGCCGGCTG CTCTGTCCGA 
AAAGAGGATG GCCTGGATGC ACAGTCGTCG CAGCGCTTTG TTGACAGAGG CTATCGTAAG 
GAAGACTTTC TGGACTATGG AGAAAAGCCT GTAAATTCCG GCGACGTGCT CCACCTGCCG 
ACCGTTCGAT TGGCCTCGAA AGGCAAGGAA TCGAAGAGTT TCCTAGTTGT GCGGCTGGTT 
CAATATCTGG AGAAAGACTG GTCCTGCGCT ACCGAGATCT GGGATTACAC TGATGATGTT 
ATTACCTGGA CGTTTTCATC ATATGACTCG GAAATGAAGG TGGACATGGA TCTTTATGTG 
AAAACAGAGA ACCGACATGG CAGCTTGATG CTTACGCTTT TTAGCCCTTT TTGGATGATC 
AATAAGACCG GCATGATGCT CACTTACAAA TCGGAAACGA CCTCCGTGGA AGTTCTCTAC 
CATCCTCCTG AGTATTCTGG TCCCATTTTG TTCACTTTCC GGGATAAGCT ATTCTTTGAC 
AAGAAAAAAG CTTCAATTCG CATAGACAAT GGTCAATGGA GTGAAAAGAT TCCATTGGAC 
GTGGCGGGTT CGGTTGGCGA AGTGATCTGC TTTGCGAACA ATCAGAAATA CCCTGTCGGT 
GTTCACAACC ACTTGACACA GAACTCTTTG ACCAAGCAGA TAACGTTCAT ACCCTTTTAT 
ATTGTGTGCA ACAAGTGTCA CTTTGATATA GAACTGCAGG AGCAGAGTCG TCCAGCCGAT 
CCATGGCTCC ATCTCGAGCC AAATGAGATG GAACCTCTCT GGCCGAGAAA CGACACAAAG 
AACAATTTGG TGGTGCGCGT GGATGGCAAG ATTACACCGG CCTTCGACTT TACGGAGGTG 
ATCTGTACGC TCCTGAAACT GGAGGACAGC AAGTACGGCG GTATCAATGT GGACGTTCAG 
ACAACGGAAG GCGGCGTCTA TATCACCTTT ACGGACTATA AGCCAGCCGA TGCGCCTGGC 
CTGCTCATCA ATCACACTGG CAAGCAGATT GTTTACCACG AGAAGGGAAC CAAAAACGAG 
CATATCCTAA ATGCAAAGAG TACTATAATG TATGCCTGGG ACGATCCTAC AGGTCCAAAA 
ATGCTTGTGT TCGGCACAAA CAAGGAAGAG ACGGATCTGA AACGAGACGG AATTGGCGAA 
GTGATCATGC AAGACGGCGG AAAGGTTCTA TGGGTATCAT TCCTCGACGG CCTGCAACGT 
GTGCTGCTCT TTACGGAAAA CGAATCGATC GCCAATCGTA CGGAAAGCAC GGCCTCGTTA 
CAGTCTATTA CCCAGAGCAT TGATCTGCGT ATTCACGGCA TTGGCTTGTC AGTGATCAAC 
AACGAGACGG GCCTTGACAT ACTTTATCTG GGAGTTACCA GCTCCGGCAT TATATGGGAG 
TCAAAGAAAG TGACCAAGAA TCGCTTTAAG GAGTTAACCA TTAACGAAAA CGCGCTTTTG 
GAAATTGAGT ATCAAAAATA TCTAGTTCAC AAGAGTGTCA ACGATGTGCA GACCTACAAG 
CTAGATAACA AGTTCCCGAT CGACTTCGAC CTTATGATCC TCAAGAAGAC GGTGGAGAGA 
AACTTAAGAC GCAGTTTCTA TCCAGCGATT TGGTTGTCCC GCAAGTCGTC GCCATTCCAA 
AGCCAGCTGC ATGTGAAAAT TAATCGTATA CAGGTGGATA ATCAGTTCCT GGATCCAATA 
TTCCCTGTGG TGTTGGCGCC AATTCCACCC CCGAAGAGTG TGGCAAGTAC CACCAGTCTT 
AAACCATTTA TCGAGTGCAG CATGGTGCAG CGCATCATGC CGAACTCAAC TGTGCGTCAG 
TTCAAGTACG CCAGGATACT GATCCAAGAG TTCCTCTTCA AAGTCGATTT GAACTTCCTT 
ACTGCCATCG CCGAAATGTT TGCCAAGGAG GTCTCGGACG AGGCAGCAGC TAAGCAGTTC 
CGTCAGGATG TTGAATCAAT TGAGCTCCCG CTTTCAGCGT TCTTTGAAGA GCACTCGTTG 
GAGGAGCAGA AGAGCTTTTA CGATAACCTG CATTTGGGTC CGCTCAAGAT CCATGTCAGC 
TTCTCGATGG CCGGGTCGGA CACAAAGGCG TTGCCCGGTT TCTTGGGCTC ATTGGTCCAG 
GGCGTGGGTG TGACTCTAAC GGACGTAAAC GATGTTGTTT TCCGTCTGGC CTTCTTCGAG 
CGCGAGTATC AATTCTTCTC CCAAAAGCAG CTAATCAACG AGATCACCTC ACACTACACT 
GGCCAAGCGT TAAAGCAGCT GTATGTGCTC GTCCTCGGAC TTGATGTCCT GGGCAATCCC 
TATGGCCTAG TGGTTGGTCT TAAGAAGGGC GTCGAAGACT TGTTCTATGA ACCGTTCCAG 
GGTGCCATCC AAGGTCCTGG AGAATTCGCC GAGGGTTTGG TCCTGGGCGT AAAGTCTCTT 
TTCGGCCACA CTGTGGGTGG TGCTGCGGGA GCGGTTTCGA AAATAACTGG TGCTATGGGA 
AAGGGTTTGG CTGCGCTGAC CTTCGACGAG GACTATCAAA AGAAACGACG ACAGGGTATT 
CAAAACAAAC CGAAGAACTT CCACGAAGGA TTGGCCCGCA GCAGCAAGGG TCTAGTGATG 
GGCTTTGTGG ACGGTGTCAC TGGCGTTGTA ACCAAACCAG TGACTGGGGC TCGCGATAAC 
GGGGTCGAGG GCTTCTTCAA GGGCCTGGGT AAGGGTGCAA TTGGCCTGGT TGCTCGCCCC 
ACCGCTGGCG TGGTTGACTT TGCCAGTGGC AGCTTCGAGG CTGTGAAACG GGCGGCGGAT 
GCAAGCGAGG ATGTGAAGCG TATGCGACCG CCGCGTTTCC AGCACTACGA CTTCGTGTTG 
AGACCCTATT GCTTGATGGA GGCGACAGGG AATAAAATAA TGAAGGAAAC CGACAAGGGT 
AAATTTGCCA CCACAGATAA CTTCATTCAC TGTGAGGAGA TTATCCAAAA GTCCGAGTAC 
TTGGTGGTGA CCAATTACCG GGTGATGTAC GTGCAACGTA ACGAGATGTT CGGCGTTTGG 
ACGTCTCTTT GGTCCTACCT ATGGAACGAG ATTAGCTCCG TGGCTGCAAC GGCTCGCGGT 
GTCCAGTTCA CAGTAAAGAC GGATGGCAAG AAGGTACTTG GTCTGTTTAG CAGCAAGGAG 
TCACCTCGAA AGTTAGTGCT CGTGGCAGAT GAGCGCAAAC GTGACGCTCT TGTGGACATT 
ATAGAATCTC AGCGCTCCGA TCCCAATCCG TTAAGGGCCA CCATCGCCTA TCCGGCTCAT 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610299.2
CDS	195..10160
Translation	MVFEAVVADVLNKVLGDYIENLDRNQLKIGIWGGDVVLQNLKIRENALDELDLPVQLIYGYLGKLVLKIPWKNLYSQPVIVNIEDLYVLVSPNNNVQYNAEKEAKYEMDLKKAALDALEAARKKELEMDQPKADAGFAEKLTAQIVNNLQVQITNVHLRYEDTTTTGSPFSFGISLHELELYTTDCDWEKCYMAQQASQVFKIANLSCLSAYLNCGGQLYANNKSDLSQQFKTNIACKETKPNYNYVLGPISCNAKLKLNMNPELDDPPFEKPKIDLTLEMEKLNVGLTNTQFDNLMKLGDAMNRQQLGIPYRKYRPYNIPYKGHARDWWHFAITSILEEEVRKPRESWTWGHIKTHRERCNTYAQKYKEQCLSKKPSAVLTETCRLLETELDVFNLLLIRQRVNIEIAKQREAVPEQKSGWFSGWGWGGGAKKDDQTTSQKLVEKFEAAMTSEEKEKMYRAIGYQENAKPTDLPESYEAIRMNFKLIALEVGLYKDERNSSAATKDFHELPSLVLLNFSMATALITQRPAAEAISIIAGMREIKVTGLTRNDYTPLLVESKITDEFNLLEVFFETNPLDKLCDQRVKVVARPLQITYDAPTILALINAFQTPGDVTLSKFEDAASTKISNFKERSATGMQYMIDKKAVLDVDILLMPNILVVPHKGVYDAGNVSLLVVSMGQVHLSSQPRRESNKLQHLFSAGEDKDEILKTVMENAYDRFTVAVDDVQMLVVRAGEPWQNALAEANSTEMHVLRPVSLKVTAALCVVDNDPRLPNIKVDIDLPAILVNVSEDRIFLAIKVATSIPLPEQKEPASRLTQTNSRSSMSISNFINKEVKKIGPSASGSSASKDPLLDEIIQYTSLDVNFSLGEINFVLFQSSRKCETSPDVSIEFLTPDGDVLPSQLTENIQEPIEELPPTPPQQILSIDIRRLEAHFVSKTYESVATVKLGDINLRQYDCQDSDMDVLDVIYTPKQENSSNYLFTVSCTIADKSSPEFSTKYNSTEQLVVANFEVLQIVLHQECLQRIMEVVNNFQRNLDLVLSSTRPRDRMGSIGGGDGIKRTLNVILEDTEEIMTTDQMKRRKKTRRTHVVETVKVRVIANLDQVGLVLTGRKRPIAEMNVKKFVSSLIIKSSYTEVNIGLKDIQVLDLNPYTIHKNILSIVGKDAFNCQIVIYNKEETQDYNSDDMKITVDIGCMKIIFLNWFVAGVMNFLNNFTAAQATISQAGAAAAESARQKAMDAYETATRMKLNIRIKAPIIIVPIGSQDRNALLLDLGLLELTNNTVEVAVAEEERLAVIDEIKLQICDVKISKIVLLDGNESTVDEVDAEVGFLSKFNMMNPMSCTLSITRNLSYTWYRDVPELNLSGRLKSIELTLFADDYALVMLVLNRNLNEGLEEFPPSEEAPQEAQVRPERRNSRAGRLSRTVQVSPIREKIHESIKFNFQFDGVVINLMEGEGAGLARFGIYFLSVKGTKLDNGTLSTSVVLCNIQMDDMRSNSKSQIRQYLSRKDWVQPKLDTDEIIDACYNERNFMVDVTAIIKEDDTFAEVRVRGFDLIVCIDFLLKLTTFLTLPPEENPRESVYIKPAPVSETARDTKHSIRSSAILAAQELVPVESSSHEVPNRKMNLILHIDEPDIILVENLEDLNTSCIIFNAQVHLNYRSINDKQIVNGQIDALKMYMCAFLPERREMTRHYILHPCVISLQGSTPEEEGMHISLKLSDIIINVSPATIELLNKAMLSVSSGTMTKCAIAEESRNYSNLWHQHHFHSRTYWFTKVEQGVDALEAEQRSVSTDNEKQKTEKCVIEIPSITLVIESGVGYYTKPLISLDTRITAVFNNWSRSLTAHGSLTLNMNYYNQALAEWEPIIELNEVIGRNGVREYTPWELKFEMGMEKVQSELEDDAEQQAMHMNIHSAETLEITLSKTCLGLLSELAEAFSQAIDQNGLTKPDIVAPYVLENDTGFDVNLNLRKGIFTLHEVHRGGTPVGANSTLLMVAQSEEVDPSVIKTCTISTGGRAYLQTKDLSTLSEEDSEDYTLYVTIGDINKEIALPVSKSDTRFFNLMRSTSHEPWGIISEVKQEYGTTKVNIHGVVSVHNHFTTGLNIYRRNPAPTAQCFEDIFVGRVRPGEVFHVPLHAIYAESKDLFFSMRGYRRSVQGISWASNPSDLNYSHQLHCDPTNTFEPLIMNARRSKSEVYFENTNKYTLLSAFYTIHLRPPLYLRNSLPINIQVSVAGCSVRKEDGLDAQSSQRFVDRGYRKEDFLDYGEKPVNSGDVLHLPTVRLASKGKESKSFLVVRLVQYLEKDWSCATEIWDYTDDVITWTFSSYDSEMKVDMDLYVKTENRHGSLMLTLFSPFWMINKTGMMLTYKSETTSVEVLYHPPEYSGPILFTFRDKLFFDKKKASIRIDNGQWSEKIPLDVAGSVGEVICFANNQKYPVGVHNHLTQNSLTKQITFIPFYIVCNKCHFDIELQEQSRPADPWLHLEPNEMEPLWPRNDTKNNLVVRVDGKITPAFDFTEVICTLLKLEDSKYGGINVDVQTTEGGVYITFTDYKPADAPGLLINHTGKQIVYHEKGTKNEHILNAKSTIMYAWDDPTGPKMLVFGTNKEETDLKRDGIGEVIMQDGGKVLWVSFLDGLQRVLLFTENESIANRTESTASLQSITQSIDLRIHGIGLSVINNETGLDILYLGVTSSGIIWESKKVTKNRFKELTINENALLEIEYQKYLVHKSVNDVQTYKLDNKFPIDFDLMILKKTVERNLRRSFYPAIWLSRKSSPFQSQLHVKINRIQVDNQFLDPIFPVVLAPIPPPKSVASTTSLKPFIECSMVQRIMPNSTVRQFKYARILIQEFLFKVDLNFLTAIAEMFAKEVSDEAAAKQFRQDVESIELPLSAFFEEHSLEEQKSFYDNLHLGPLKIHVSFSMAGSDTKALPGFLGSLVQGVGVTLTDVNDVVFRLAFFEREYQFFSQKQLINEITSHYTGQALKQLYVLVLGLDVLGNPYGLVVGLKKGVEDLFYEPFQGAIQGPGEFAEGLVLGVKSLFGHTVGGAAGAVSKITGAMGKGLAALTFDEDYQKKRRQGIQNKPKNFHEGLARSSKGLVMGFVDGVTGVVTKPVTGARDNGVEGFFKGLGKGAIGLVARPTAGVVDFASGSFEAVKRAADASEDVKRMRPPRFQHYDFVLRPYCLMEATGNKIMKETDKGKFATTDNFIHCEEIIQKSEYLVVTNYRVMYVQRNEMFGVWTSLWSYLWNEISSVAATARGVQFTVKTDGKKVLGLFSSKESPRKLVLVADERKRDALVDIIESQRSDPNPLRATIAYPAHN

Target ORF information:

RefSeq Version	NM_136455.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 13, transcript variant A (Vps13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136455.3

ORF Insert Sequence:

ATGGTCTTCG AGGCGGTGGT GGCCGATGTG CTGAACAAGG TGCTCGGCGA CTACATCGAG 
AACTTGGACC GGAACCAGCT GAAGATTGGC ATTTGGGGAG GCGATGTCGT CTTGCAGAAC 
CTCAAGATAC GGGAGAATGC CCTGGACGAG TTGGACCTGC CCGTACAGCT TATCTATGGT 
TACTTGGGCA AACTAGTGCT TAAGATCCCC TGGAAGAATT TGTACAGCCA GCCGGTAATT 
GTCAACATTG AGGATCTTTA TGTCTTAGTT TCACCCAACA ACAATGTCCA GTACAACGCC 
GAAAAGGAGG CCAAATATGA GATGGACCTC AAGAAGGCGG CGCTTGATGC CCTGGAGGCA 
GCTCGCAAAA AGGAGTTGGA AATGGATCAG CCCAAAGCAG ATGCAGGATT TGCCGAAAAG 
CTAACGGCGC AGATCGTTAA CAATCTGCAG GTGCAGATAA CCAACGTTCA TCTGCGATAC 
GAGGATACCA CAACGACGGG TAGTCCCTTC TCCTTCGGCA TATCCCTGCA TGAGCTCGAA 
CTTTACACCA CCGATTGCGA CTGGGAGAAG TGCTACATGG CCCAGCAGGC GTCGCAGGTC 
TTCAAAATAG CGAATCTCTC ATGTCTTTCC GCCTATTTGA ACTGTGGTGG ACAACTGTAC 
GCGAACAACA AGAGCGACCT TTCACAGCAG TTCAAGACGA ATATTGCTTG CAAGGAAACA 
AAACCCAACT ACAACTATGT GCTGGGTCCT ATCTCTTGCA ATGCCAAGCT AAAGCTAAAC 
ATGAACCCGG AGCTCGATGA TCCGCCATTT GAAAAACCTA AAATCGATCT TACGCTGGAA 
ATGGAAAAGC TTAATGTCGG CTTGACAAAC ACACAGTTCG ACAATCTCAT GAAGCTTGGA 
GACGCTATGA ACCGCCAGCA GTTGGGCATA CCGTACCGGA AATATCGTCC CTACAATATA 
CCATACAAAG GCCACGCCAG GGACTGGTGG CACTTTGCAA TAACTTCTAT TCTAGAGGAG 
GAAGTAAGGA AACCCAGAGA GAGCTGGACT TGGGGACACA TCAAGACCCA CAGAGAACGC 
TGCAACACTT ATGCACAGAA ATACAAGGAG CAGTGCTTAA GCAAAAAACC GTCAGCCGTG 
CTTACGGAAA CCTGCCGTTT GCTAGAAACG GAGCTGGACG TTTTTAACTT GCTGCTAATC 
CGGCAGAGGG TCAACATCGA GATAGCCAAG CAACGAGAGG CAGTGCCTGA ACAAAAGTCG 
GGCTGGTTCA GTGGTTGGGG ATGGGGCGGC GGAGCCAAGA AGGATGACCA AACCACCAGT 
CAAAAACTTG TTGAGAAGTT TGAAGCGGCA ATGACCAGTG AAGAGAAAGA GAAGATGTAC 
AGAGCTATTG GTTACCAAGA GAACGCCAAG CCCACAGACC TTCCAGAGAG CTACGAGGCC 
ATCAGGATGA ACTTTAAACT GATAGCCTTA GAGGTCGGCC TTTACAAGGA TGAGCGTAAT 
AGTTCCGCGG CAACGAAGGA CTTCCATGAG CTGCCGTCGC TGGTCCTTCT TAACTTTTCT 
ATGGCCACCG CGTTGATCAC CCAACGGCCA GCTGCCGAAG CCATCAGCAT TATTGCTGGC 
ATGAGAGAAA TCAAGGTAAC TGGTCTCACG CGCAACGATT ACACGCCTTT ATTGGTTGAG 
TCAAAAATCA CCGACGAGTT CAACCTGCTG GAAGTGTTTT TCGAGACAAA CCCACTGGAC 
AAGTTGTGCG ACCAGCGAGT GAAGGTTGTG GCGCGCCCGT TGCAGATAAC GTATGATGCG 
CCAACCATAT TGGCCCTGAT AAACGCATTC CAGACGCCTG GCGATGTCAC GCTCTCCAAG 
TTTGAGGATG CTGCCAGCAC GAAAATCTCG AACTTCAAGG AGCGTTCAGC CACTGGAATG 
CAGTACATGA TCGACAAGAA GGCAGTGCTG GATGTGGACA TCCTGTTAAT GCCAAACATC 
CTGGTCGTAC CACATAAAGG AGTCTACGAT GCGGGCAACG TATCCCTGCT AGTAGTTAGC 
ATGGGCCAGG TGCACCTCTC CAGCCAACCG CGTAGGGAGT CGAACAAGCT GCAGCATCTT 
TTCTCAGCCG GCGAGGATAA GGATGAGATC CTCAAGACGG TAATGGAAAA TGCTTATGAC 
AGGTTTACAG TCGCAGTTGA CGATGTGCAG ATGCTGGTCG TGCGAGCTGG AGAGCCGTGG 
CAAAATGCGC TAGCGGAGGC AAACTCAACG GAGATGCACG TTTTGCGCCC CGTCTCCCTA 
AAAGTAACTG CCGCACTATG TGTGGTTGAC AATGATCCGC GTCTTCCAAA TATTAAAGTG 
GACATCGACT TGCCTGCGAT CCTAGTTAAT GTCTCAGAGG ATCGCATTTT CCTCGCCATA 
AAGGTGGCAA CAAGTATTCC CCTGCCGGAG CAAAAGGAAC CGGCTAGTAG ACTCACACAG 
ACGAACTCGC GCAGCTCAAT GTCCATATCC AATTTCATCA ACAAGGAAGT GAAAAAGATT 
GGGCCTTCGG CATCGGGGAG CTCTGCCAGT AAGGATCCGT TGCTGGACGA GATCATTCAG 
TACACCAGTC TCGATGTAAA TTTTTCTCTT GGAGAAATCA ACTTCGTTTT GTTCCAGTCC 
AGTAGAAAAT GCGAGACAAG CCCCGATGTT TCTATTGAAT TTCTGACTCC TGATGGTGAT 
GTCCTTCCTT CTCAGTTGAC TGAGAATATC CAGGAGCCTA TTGAAGAGCT ACCCCCAACG 
CCACCTCAGC AGATTCTCTC AATCGACATT CGACGCCTGG AGGCCCACTT TGTGTCCAAA 
ACCTATGAAT CGGTGGCCAC AGTCAAACTT GGAGATATCA ACCTGCGACA GTATGACTGC 
CAGGACAGTG ACATGGATGT ACTAGATGTT ATCTATACGC CGAAACAGGA GAATAGTTCC 
AATTACCTGT TCACCGTCTC GTGCACAATT GCAGACAAGT CGTCGCCAGA GTTCAGCACC 
AAGTACAACT CCACTGAACA GTTGGTCGTG GCTAACTTCG AGGTACTTCA AATCGTGCTG 
CACCAGGAAT GCCTACAAAG GATTATGGAG GTCGTAAACA ATTTCCAAAG AAACCTAGAT 
CTTGTACTAT CAAGTACCCG ACCACGGGAT CGGATGGGCT CCATTGGGGG AGGAGATGGC 
ATTAAACGTA CGCTTAATGT TATTCTTGAG GACACAGAGG AAATAATGAC CACCGACCAG 
ATGAAGCGGC GAAAGAAGAC GCGCCGCACT CACGTCGTAG AGACGGTGAA AGTTCGTGTT 
ATCGCTAATT TGGATCAAGT GGGTCTGGTG CTGACCGGCC GAAAGCGGCC GATAGCAGAG 
ATGAATGTGA AGAAATTCGT GTCCAGTCTG ATCATAAAGT CGTCCTACAC GGAAGTCAAC 
ATTGGCCTTA AAGACATTCA GGTGTTGGAC CTAAATCCTT ACACTATTCA CAAGAATATT 
CTGAGTATTG TTGGCAAGGA CGCTTTCAAC TGCCAGATTG TGATCTACAA CAAGGAGGAG 
ACCCAAGACT ACAACTCTGA TGATATGAAA ATAACCGTGG ACATTGGCTG CATGAAGATC 
ATCTTCCTCA ATTGGTTTGT GGCAGGTGTT ATGAACTTCT TAAACAACTT CACAGCGGCC 
CAAGCAACCA TATCGCAGGC TGGTGCTGCG GCTGCCGAGT CCGCTCGCCA GAAGGCAATG 
GATGCCTATG AAACGGCCAC TCGTATGAAA CTAAATATCC GCATCAAGGC TCCCATTATC 
ATTGTGCCAA TTGGGTCACA GGACAGGAAT GCCTTGCTGC TCGATTTGGG CTTACTGGAG 
CTAACGAATA ACACGGTGGA GGTGGCGGTG GCCGAAGAGG AACGGCTAGC TGTTATAGAC 
GAGATCAAGT TGCAAATATG CGATGTTAAG ATTTCCAAAA TTGTCTTATT AGATGGCAAC 
GAGTCAACGG TTGATGAAGT GGACGCGGAA GTGGGTTTCC TCTCTAAATT CAATATGATG 
AATCCCATGA GTTGCACCTT ATCAATCACG CGCAATTTGT CATACACATG GTACCGGGAT 
GTGCCCGAAC TAAATCTTTC CGGTCGATTA AAGTCCATTG AGCTCACTCT ATTCGCGGAT 
GATTATGCTT TGGTAATGCT TGTTCTCAAT CGCAATTTAA ACGAGGGCTT GGAAGAGTTC 
CCTCCAAGCG AGGAGGCGCC GCAGGAAGCA CAAGTTAGAC CCGAAAGACG CAACTCTCGT 
GCGGGACGTT TATCTCGTAC CGTTCAAGTT TCTCCGATAA GGGAAAAGAT TCACGAATCA 
ATCAAGTTTA ACTTTCAGTT TGACGGTGTG GTCATAAATT TAATGGAGGG TGAAGGAGCA 
GGACTCGCCA GGTTTGGAAT ATATTTCCTC TCCGTTAAGG GCACCAAGTT GGACAACGGC 
ACACTGAGCA CTTCTGTTGT ATTGTGCAAT ATTCAAATGG ATGACATGCG AAGTAATTCC 
AAAAGCCAGA TCCGACAGTA CTTGAGTCGC AAGGATTGGG TTCAGCCTAA GTTGGATACA 
GACGAGATCA TCGATGCCTG TTACAACGAG CGCAACTTCA TGGTCGATGT GACGGCTATA 
ATAAAAGAAG ATGACACGTT CGCTGAGGTC CGTGTGCGTG GCTTCGATCT GATCGTCTGC 
ATCGACTTCC TGCTGAAACT GACTACGTTT CTTACCTTGC CGCCTGAGGA GAATCCGCGC 
GAGTCTGTTT ATATAAAGCC AGCGCCTGTT TCGGAAACTG CTCGGGATAC CAAGCACTCT 
ATTCGCTCCT CCGCCATATT AGCCGCCCAA GAGCTGGTTC CTGTCGAGTC ATCCAGTCAC 
GAAGTTCCAA ACCGAAAAAT GAATTTAATA CTGCATATTG ACGAGCCAGA TATAATATTA 
GTGGAAAATC TGGAAGATCT TAATACCAGT TGTATTATTT TCAATGCTCA AGTGCATTTG 
AATTACCGCA GCATAAACGA CAAACAGATT GTAAACGGAC AGATCGATGC TCTGAAGATG 
TACATGTGTG CTTTCCTACC AGAGCGGCGA GAGATGACGC GTCATTATAT CCTTCACCCG 
TGCGTTATTA GCCTGCAAGG ATCAACTCCC GAAGAAGAGG GCATGCACAT TAGCCTAAAG 
CTCAGTGACA TTATCATCAA CGTGTCGCCG GCCACCATCG AGCTGCTAAA CAAGGCAATG 
CTGAGCGTCT CCAGTGGCAC CATGACCAAG TGCGCTATTG CCGAGGAGTC GCGGAACTAT 
TCGAATCTGT GGCATCAGCA CCACTTCCAT AGCCGCACGT ATTGGTTCAC CAAGGTGGAA 
CAGGGCGTCG ATGCCCTGGA GGCTGAGCAG AGAAGCGTCA GCACTGACAA CGAAAAGCAG 
AAGACAGAAA AGTGTGTAAT TGAAATTCCC AGCATTACGC TCGTGATTGA GTCCGGCGTA 
GGCTACTACA CGAAGCCACT TATTTCGCTA GACACCCGCA TTACTGCCGT TTTCAACAAT 
TGGAGCCGCA GTCTCACCGC TCATGGCTCG TTGACACTAA ATATGAATTA CTATAACCAA 
GCCCTTGCCG AATGGGAGCC TATTATTGAG CTGAACGAGG TCATTGGTCG CAACGGCGTT 
CGTGAATACA CGCCATGGGA GCTTAAGTTC GAAATGGGCA TGGAGAAGGT GCAGAGCGAA 
TTGGAAGACG ATGCTGAACA GCAGGCGATG CACATGAACA TCCACTCGGC AGAGACGCTG 
GAGATTACGT TGAGCAAGAC GTGCCTGGGT CTATTGAGCG AACTGGCCGA GGCCTTCTCG 
CAGGCTATTG ACCAGAACGG TCTTACCAAG CCAGACATCG TGGCTCCTTA CGTGCTGGAA 
AACGACACTG GCTTCGATGT TAACCTGAAT CTGCGGAAGG GCATCTTTAC ACTGCACGAA 
GTGCACCGCG GCGGCACACC TGTGGGTGCA AATAGTACCC TTCTTATGGT GGCCCAATCT 
GAAGAGGTCG ATCCCAGCGT CATAAAAACC TGCACCATTT CGACGGGCGG AAGGGCATAT 
CTGCAGACTA AGGATCTGAG CACGTTATCA GAAGAGGATT CCGAGGACTA CACTCTTTAT 
GTGACGATTG GAGATATCAA CAAGGAGATC GCACTGCCCG TTTCCAAGTC GGACACGCGT 
TTCTTCAACC TGATGCGCAG TACCTCTCAC GAGCCTTGGG GCATTATTTC TGAAGTAAAG 
CAGGAGTACG GCACAACCAA AGTCAATATC CATGGTGTTG TTAGTGTTCA TAATCACTTT 
ACGACCGGCT TGAATATTTA TCGACGCAAT CCTGCCCCTA CTGCACAATG TTTCGAGGAT 
ATTTTCGTCG GACGTGTGCG TCCCGGCGAA GTGTTCCATG TACCACTGCA CGCCATATAC 
GCGGAATCCA AGGATTTATT CTTTTCCATG CGCGGCTACA GAAGATCAGT GCAGGGCATT 
TCGTGGGCTT CCAATCCCTC GGACCTTAAC TATTCGCATC AGTTGCATTG TGATCCGACC 
AATACGTTCG AACCGCTTAT CATGAACGCT CGAAGATCAA AATCGGAGGT GTACTTCGAA 
AACACCAACA AGTATACATT GCTAAGCGCC TTCTACACGA TTCATCTGCG CCCTCCACTC 
TATCTGCGCA ACTCTCTGCC CATTAATATC CAAGTGTCAG TTGCCGGCTG CTCTGTCCGA 
AAAGAGGATG GCCTGGATGC ACAGTCGTCG CAGCGCTTTG TTGACAGAGG CTATCGTAAG 
GAAGACTTTC TGGACTATGG AGAAAAGCCT GTAAATTCCG GCGACGTGCT CCACCTGCCG 
ACCGTTCGAT TGGCCTCGAA AGGCAAGGAA TCGAAGAGTT TCCTAGTTGT GCGGCTGGTT 
CAATATCTGG AGAAAGACTG GTCCTGCGCT ACCGAGATCT GGGATTACAC TGATGATGTT 
ATTACCTGGA CGTTTTCATC ATATGACTCG GAAATGAAGG TGGACATGGA TCTTTATGTG 
AAAACAGAGA ACCGACATGG CAGCTTGATG CTTACGCTTT TTAGCCCTTT TTGGATGATC 
AATAAGACCG GCATGATGCT CACTTACAAA TCGGAAACGA CCTCCGTGGA AGTTCTCTAC 
CATCCTCCTG AGTATTCTGG TCCCATTTTG TTCACTTTCC GGGATAAGCT ATTCTTTGAC 
AAGAAAAAAG CTTCAATTCG CATAGACAAT GGTCAATGGA GTGAAAAGAT TCCATTGGAC 
GTGGCGGGTT CGGTTGGCGA AGTGATCTGC TTTGCGAACA ATCAGAAATA CCCTGTCGGT 
GTTCACAACC ACTTGACACA GAACTCTTTG ACCAAGCAGA TAACGTTCAT ACCCTTTTAT 
ATTGTGTGCA ACAAGTGTCA CTTTGATATA GAACTGCAGG AGCAGAGTCG TCCAGCCGAT 
CCATGGCTCC ATCTCGAGCC AAATGAGATG GAACCTCTCT GGCCGAGAAA CGACACAAAG 
AACAATTTGG TGGTGCGCGT GGATGGCAAG ATTACACCGG CCTTCGACTT TACGGAGGTG 
ATCTGTACGC TCCTGAAACT GGAGGACAGC AAGTACGGCG GTATCAATGT GGACGTTCAG 
ACAACGGAAG GCGGCGTCTA TATCACCTTT ACGGACTATA AGCCAGCCGA TGCGCCTGGC 
CTGCTCATCA ATCACACTGG CAAGCAGATT GTTTACCACG AGAAGGGAAC CAAAAACGAG 
CATATCCTAA ATGCAAAGAG TACTATAATG TATGCCTGGG ACGATCCTAC AGGTCCAAAA 
ATGCTTGTGT TCGGCACAAA CAAGGAAGAG ACGGATCTGA AACGAGACGG AATTGGCGAA 
GTGATCATGC AAGACGGCGG AAAGGTTCTA TGGGTATCAT TCCTCGACGG CCTGCAACGT 
GTGCTGCTCT TTACGGAAAA CGAATCGATC GCCAATCGTA CGGAAAGCAC GGCCTCGTTA 
CAGTCTATTA CCCAGAGCAT TGATCTGCGT ATTCACGGCA TTGGCTTGTC AGTGATCAAC 
AACGAGACGG GCCTTGACAT ACTTTATCTG GGAGTTACCA GCTCCGGCAT TATATGGGAG 
TCAAAGAAAG TGACCAAGAA TCGCTTTAAG GAGTTAACCA TTAACGAAAA CGCGCTTTTG 
GAAATTGAGT ATCAAAAATA TCTAGTTCAC AAGAGTGTCA ACGATGTGCA GACCTACAAG 
CTAGATAACA AGTTCCCGAT CGACTTCGAC CTTATGATCC TCAAGAAGAC GGTGGAGAGA 
AACTTAAGAC GCAGTTTCTA TCCAGCGATT TGGTTGTCCC GCAAGTCGTC GCCATTCCAA 
AGCCAGCTGC ATGTGAAAAT TAATCGTATA CAGGTGGATA ATCAGTTCCT GGATCCAATA 
TTCCCTGTGG TGTTGGCGCC AATTCCACCC CCGAAGAGTG TGGCAAGTAC CACCAGTCTT 
AAACCATTTA TCGAGTGCAG CATGGTGCAG CGCATCATGC CGAACTCAAC TGTGCGTCAG 
TTCAAGTACG CCAGGATACT GATCCAAGAG TTCCTCTTCA AAGTCGATTT GAACTTCCTT 
ACTGCCATCG CCGAAATGTT TGCCAAGGAG GTCTCGGACG AGGCAGCAGC TAAGCAGTTC 
CGTCAGGATG TTGAATCAAT TGAGCTCCCG CTTTCAGCGT TCTTTGAAGA GCACTCGTTG 
GAGGAGCAGA AGAGCTTTTA CGATAACCTG CATTTGGGTC CGCTCAAGAT CCATGTCAGC 
TTCTCGATGG CCGGGTCGGA CACAAAGGCG TTGCCCGGTT TCTTGGGCTC ATTGGTCCAG 
GGCGTGGGTG TGACTCTAAC GGACGTAAAC GATGTTGTTT TCCGTCTGGC CTTCTTCGAG 
CGCGAGTATC AATTCTTCTC CCAAAAGCAG CTAATCAACG AGATCACCTC ACACTACACT 
GGCCAAGCGT TAAAGCAGCT GTATGTGCTC GTCCTCGGAC TTGATGTCCT GGGCAATCCC 
TATGGCCTAG TGGTTGGTCT TAAGAAGGGC GTCGAAGACT TGTTCTATGA ACCGTTCCAG 
GGTGCCATCC AAGGTCCTGG AGAATTCGCC GAGGGTTTGG TCCTGGGCGT AAAGTCTCTT 
TTCGGCCACA CTGTGGGTGG TGCTGCGGGA GCGGTTTCGA AAATAACTGG TGCTATGGGA 
AAGGGTTTGG CTGCGCTGAC CTTCGACGAG GACTATCAAA AGAAACGACG ACAGGGTATT 
CAAAACAAAC CGAAGAACTT CCACGAAGGA TTGGCCCGCA GCAGCAAGGG TCTAGTGATG 
GGCTTTGTGG ACGGTGTCAC TGGCGTTGTA ACCAAACCAG TGACTGGGGC TCGCGATAAC 
GGGGTCGAGG GCTTCTTCAA GGGCCTGGGT AAGGGTGCAA TTGGCCTGGT TGCTCGCCCC 
ACCGCTGGCG TGGTTGACTT TGCCAGTGGC AGCTTCGAGG CTGTGAAACG GGCGGCGGAT 
GCAAGCGAGG ATGTGAAGCG TATGCGACCG CCGCGTTTCC AGCACTACGA CTTCGTGTTG 
AGACCCTATT GCTTGATGGA GGCGACAGGG AATAAAATAA TGAAGGAAAC CGACAAGGGT 
AAATTTGCCA CCACAGATAA CTTCATTCAC TGTGAGGAGA TTATCCAAAA GTCCGAGTAC 
TTGGTGGTGA CCAATTACCG GGTGATGTAC GTGCAACGTA ACGAGATGTT CGGCGTTTGG 
ACGTCTCTTT GGTCCTACCT ATGGAACGAG ATTAGCTCCG TGGCTGCAAC GGCTCGCGGT 
GTCCAGTTCA CAGTAAAGAC GGATGGCAAG AAGGTACTTG GTCTGTTTAG CAGCAAGGAG 
TCACCTCGAA AGTTAGTGCT CGTGGCAGAT GAGCGCAAAC GTGACGCTCT TGTGGACATT 
ATAGAATCTC AGCGCTCCGA TCCCAATCCG TTAAGGGCCA CCATCGCCTA TCCGGCTCAT 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vps13 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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