VPS13A cDNA ORF clone, Danio rerio(Zebrafish)

The following VPS13A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS13A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa40323 XM_009304311.1
Latest version!
Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa34180 NM_001118893.2
Latest version!
Danio rerio vacuolar protein sorting 13 homolog A (vps13a), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa14675 XM_005167334.4
Latest version!
Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa40321 XM_009304309.3
Latest version!
Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa40322 XM_009304310.3
Latest version!
Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa14675 XM_005167334.3 Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa40322 XM_009304310.2 Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa40321 XM_009304309.2 Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa40323
    Clone ID Related Accession (Same CDS sequence) XM_009304311.1
    Accession Version XM_009304311.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product vacuolar protein sorting-associated protein 13A isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATCACAT CCAGGATGAT GGCGATGCGT CAGGACCGAG AGGACAGTGT GATGGTGGAC 
    GTCAGCTACC GCCAGAGTCG GGACGGGACT CGTCTGGACA CCCTCGTGCA AGACCTTTAT
    CTTTGTGCCA GTATGGAGTT TCTGCTCACT GTAGCTGATG TGTTTCTTAA AGCTCTGTCT
    GACGGCTTCT CCGGTCCTCC AAAGAGTAAC AAACAACCGG CCAGCACCTC TAAAGACCCA
    GCGGCTCCTG CTCCACCAGT GGTTGCTAAA ACCGAGATGA ATGTGGTGGT GAAGAACCCA
    GAGATCGTGT TTGTGGCGGA TCTGACGCGT GCTGAAGCTC CGGCTCTGGT CATGACCACT
    CAGTGTGAGC TGGTGATGAA GAGCGAGCCC AGCGGTCAGT GCATGACGGC CGCCATTTCA
    GACCTGAAGA TTGTGGCCTG TCCGTTCCTG CGGGAGAAGA GGGAGAACAA CGTGACGACG
    GTCCTACAGC CCTGCGAGGT TTTCTTCCAG AGCCGACAGT CAAACACTGA GCCGCAGTGC
    ATCGAGCTGT CCATCAGCGC ACTCACGCTC AAGGTGTCTC CGATCATCAT AAACACGGTC
    ATCACCATTC AGACGGCTCT AAGTCCTGCC GCTGAGACGC CGCAGGAGCT GGAGAGCCCC
    ATCCCACAGG ACCTATGGGA GCGCCGCAGC CTTCGCGACC TGAAGCTCTG GTTCCTGGAG
    GAGGAGGGCG AGGAGGTCAG CACGTCCGTC ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG
    AAGATGAGCA TCGCCTCCAT CTGCCTGACG CTGGAGGCCG GAGTGGGACA CCGCACCGTC
    CCCATGCTGC TGGCCAAAGC CTCGTTCCAG GGGGACGTCA AGGACTGGAG CACTCTCATC
    AACCTGCACT GCCACCTCAG CATGGAGGTG AACTACTATA ATGAGATGAT GGGTTTATGG
    GAGCCGCTGT TGGAGCCGCT GGACGATGAG GATAAAGACG GTTTTAGACC CTGGACTCTC
    GCACTGAAGA TGAAGAAGAA GCCGGTGGTC AGTTCTGGGG TGTCTGATGA GGTGGACGGC
    GCTGTGCCTG ATTATAAAAC GGTCATCAGC TTCACTAGTA AAGACCAGCT TAACGTCACC
    CTGTCCAAGT GTGGTCTGCA CATGCTCAGT AATCTGGGAA CGGCTTTTGC AGAGGCCGCT
    AAACAGACGG CCGAGTGCTT TCAGACAGAT CAGACACCAT TCGTGGTGAA GAATCGCCTG
    GGTTTGGCGG TGTCTGTGCT GCACAGCGAA GTGTTTCGAC CCATCGGGAA GGAGTCTAAG
    GATCGTGTGG TGGAGCTCCC GGATGGAGAG AGTCTGAATT TGGACTATAC CCTCACCGCC
    GAGTCCAGCG ACCAGTTCTC TGCCATGACC AGCCTCAGTG CCAAAAACTA CATACAGCCC
    ACTCCAGTCG GCCACACGAC CGCAAGTGTG ATCCCGCTCA TCAAAACGGG CCGGGGCATG
    TACAGCGTCA TGCACAACGA GTCCCGCGTC ACTCGCTTCT TCGTCTGTCA GATTTACACC
    GTCGAGGGCA GCAAATATGT CAAGATTCGC TCTCCCTTCC AGATTATCAA CCACTTTTCA
    GTGCCGTTCC GTGTTTATGA AGGATCCGTG TTTCTGGGCG AGTCTCTGCC GACAGAGGAG
    TTCTGTGTGC CGCTGGGATC CTACAGAGCT GAGCTGAGTC TGCAGCCTGT GCCTGATGGC
    GGTGAAGAGT ACGAGCACTC CGAGAGCTTC ACCTACACTG AGGTTGTCCA GAAACCCGGA
    AAGTGTCTGG AGAAAAACTG CCATCTGCGT GGTGACAGCG CCCAGATTCT GAAGGTCAAC
    ATGGTTCCTC TTGCGGACAC AGTGACGTCC CACAGCAGCG AGGAGGAGTT CGATAAGCCC
    ATCGTACTGC ATTTGTGGCC CACCGTCCTG CTGCGCAACC TGCTGCCCTA CCCAATTACT
    TTCAAGCTAA AGGGCATCGG AGATTCCCCC TCGGTGTCGC TGAAGGAGGG CCACTCATCC
    CAGATCCACA CCGCCATCAT GGACTTCTCC GTGTTGGATC TGAGGCTGCT GGGATACTTG
    CAGCAGGACT GGGAGTCTCA GTACAGCCTC AGCAGTGAAC AAGACGAGAT CAGCTTCATT
    AAGTTTCGCT CGCTCGCACG TGAGGAGGAG ACTGAGAGCC GGCCGGAGCT GGACATCGCT
    ATGCATGTGA AGAAGGAGGA GGGTCAGGTT GTGCTGGCCG TACACTCGCC GTACTGGATG
    GTGAATAAAA CAAGCCGACT GCTGCAGTAC AAGGCGGATG ATATTCATCG CAAACACCCC
    AAGGATTACG ACATGCCGCT GCTCTTCTCC TTCAAGCCAC GCAACTTCCT GCAAAACAAC
    AAGATCCGCC TGATGATCTC AGAAAGTGAG CTGTCAGATG ATTTCTCCAT CGACACTGTC
    GGGAGTTACG GAGATGTCAA ATGCAAAGGC AGACAGAAGG ATTACTTGGT GGGAGTGAAG
    ATCGATGCTA GCAGCTTCAG CTTGACACGT TTGGTGACTT TTCTGCCATT CTACATGCTG
    GTGAACAGAA CCAAACATGT GATTCGGGTC TGTGAGGAGG GGCAGGAGAA CTGGACAGAA
    GCCCGACCCG GACAGGCTGC TGTTCCATTC TGGCCAGAAA GAGACTCAAA GAAGCTGAGA
    GTGAAGGTGG AGGGCAGTCA GTCGTCACCA CGCGCCTTTG ACTTCCACCA GCCAGAGAAC
    TGCCTCCTGC TGCATCTGGA CAAAAGCTTG GGTGGCATCA TTGTGGATGT GAATCTGACA
    GAGCATTCAG CTGTGATCCT CTTCTCTGAT TATCATGATG GAGCCGCTCC CTTCCTCCTC
    ATCAACCACA CTAAGGACGA GACGCTGCAG TACCACCAGA GCAACAGTAA GAGGAAGCCT
    TCGACGTGGT TTCAGGATGA AATACTGGAT TTATCAATGA GGGATGAGAC TGATTCCCTC
    TGCTCTCAGG CCGAGATCGA TGAACTGAGT CCAGGTCAGG CGGTTTACTA CACCTGGGCT
    GAACCAACCG GCTCCAGGAT CCTCAGCTGG AAACTGGGCA AATACAAAGG AGAGCTCAAG
    AGTGAAGAGG ATCAGTTGCT GGATGTGGAC AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT
    TATGAAGGCC TCCAGCGTGT GGCGCTCTTC ACCAGCGAGC AGCGCATCTT CAAGAAGATC
    TGCGACAGTG AGAAGATAGA GCTGGCTCAG CAAGAAATCA GCATCTTGCT GCAGAACATG
    GGCATTTCGC TGGTCAACAA CAACTCCAGC CAGGAAGTGG CCTTCATTGG CATCACCAGC
    TCTGATGTTG TGTGGGAAAC CAAGCCGAAG AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT
    AAAGAGGCAG CAGTTCTGGA GCAGCACTAC AGAGACTACA TGGAGAACGG CCCAGTGGAC
    AGCACCATCA TAGACCTGGA CAACGACTAC CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC
    ATGAGGATCA TGCAGCCCTA CGATGCTCCA CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG
    GTGGAGTACA GCGTTTCAGC CCGCCAGAAA ACATACAGAG TCCAGATCAA CAGGATCCAG
    ATCCAAAACC AGCTGCAAGG AGCCATATTC CCGTTTGTGT TTTACCCCAT CAAACCGCCA
    AAGTCTGTCA GCATGGACTC AGAGCCAAAA CCGCTGGCTG ACGTGAGCAT CATCACCAGA
    GCAGCAGGAC ACTCCGACAT TCTGCGCATC AAATATTTTA TGGTGTTGAT TCAGGAGATG
    GACCTGCGGC TGGATCTGGG CTTTCTGTAT GCCATACTTG AGCTCTTCAC TGCTGAACAC
    ACCAGTGCTG TGACGTCTGA ACAGGAGGTG GAGTTGTTTG ATAAAGACAT AGAGTATCTA
    AAGACTGAGT TAAATCATGT TTCAGCTACA GACACATCTC CCATCAGCCT CTACGAGTAC
    TTCCACATCT CTCCTATAAA GCTTCACTTG AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC
    AGCAATAAGC AAGAGCGCGA GACGGAGCTG ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC
    AAGAGCATCG GGGCTACACT TACAGACGCA CAGGACGTCC TCTTTAAACT GGGCTTCTTC
    GAGCTGAGGT ATCAGTTTCG CACCACTCAG CAGCTGCAGT GGGAGGTCGT TCGCCATTAC
    TCCAAACAGG CTATCAAGCA GATGTATGTC CTTGTTCTGG GTTTGGACGT TCTTGGGAAT
    CCATTCGGGC TGATCCGGGG CCTGTCTGAA GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT
    CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT
    CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC GGAGCGGCGT CTCGCATCAC TGGAGCGATG
    GCTAAAGGTG TGGCGGCGAT GACGATGGAT GAAGAGTACC AGCAGAAGAG ACGAGAGGCC
    ATGAACAAAC AGCCCAGCGG ACTGAGAGAA GGACTGACAC GAGGAGGAAA AGGACTGGTG
    TCTGGCTTCG TGAGTGGCAT CACGGGCATT GTGACCAAAC CCATTAAAGG AGCTCAGAAG
    GAAGGAGCGG CAGGTTTCTT CAAGGGTATG GGTAAGGGTT TAGTTGGAGC TCTGACCAGA
    CCAACAGGGG GCATCATTGA CATGGCCAGC AGCACCTTCC AGGGCATCAA GAGAGCTGCA
    GAGCTGTCTC AGGATGTGGA GAGTCTGCGC CCTCCACGCT TCATCCACGA GGACGGAGTG
    ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC GGCAGTCAGA TGCTGCAGAG TATTGAAAAC
    GGACGGTTTG CCAAGTACAG GTACTTTGCT CACGCTAAAG TCAACGAGTC TGATTTCCTG
    ATGATCACTA AAAGGGGGAT CTTTTTCGTG ACAAAGGGCA CGTTTGGTCA GTTGACATGT
    GAATGGCAGT ATCTGTTCGA TGAGTTTACT AAAGCACCCA TGATTGTGGA AGGCAGGAGA
    CTGCGCATCG AAGCGAAGGA GAGAGTCAAG TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG
    ATCATCAATT TCAAAACTCC AGAATTAGCA AAGTGGGTTC TTGCCAAGCT TGAAGACGCT
    CGGGAGAATC TGCCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302586.1
    CDS1..5241
    Translation

    Target ORF information:

    RefSeq Version XM_009304311.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304311.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATCACAT CCAGGATGAT GGCGATGCGT CAGGACCGAG AGGACAGTGT GATGGTGGAC 
    GTCAGCTACC GCCAGAGTCG GGACGGGACT CGTCTGGACA CCCTCGTGCA AGACCTTTAT
    CTTTGTGCCA GTATGGAGTT TCTGCTCACT GTAGCTGATG TGTTTCTTAA AGCTCTGTCT
    GACGGCTTCT CCGGTCCTCC AAAGAGTAAC AAACAACCGG CCAGCACCTC TAAAGACCCA
    GCGGCTCCTG CTCCACCAGT GGTTGCTAAA ACCGAGATGA ATGTGGTGGT GAAGAACCCA
    GAGATCGTGT TTGTGGCGGA TCTGACGCGT GCTGAAGCTC CGGCTCTGGT CATGACCACT
    CAGTGTGAGC TGGTGATGAA GAGCGAGCCC AGCGGTCAGT GCATGACGGC CGCCATTTCA
    GACCTGAAGA TTGTGGCCTG TCCGTTCCTG CGGGAGAAGA GGGAGAACAA CGTGACGACG
    GTCCTACAGC CCTGCGAGGT TTTCTTCCAG AGCCGACAGT CAAACACTGA GCCGCAGTGC
    ATCGAGCTGT CCATCAGCGC ACTCACGCTC AAGGTGTCTC CGATCATCAT AAACACGGTC
    ATCACCATTC AGACGGCTCT AAGTCCTGCC GCTGAGACGC CGCAGGAGCT GGAGAGCCCC
    ATCCCACAGG ACCTATGGGA GCGCCGCAGC CTTCGCGACC TGAAGCTCTG GTTCCTGGAG
    GAGGAGGGCG AGGAGGTCAG CACGTCCGTC ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG
    AAGATGAGCA TCGCCTCCAT CTGCCTGACG CTGGAGGCCG GAGTGGGACA CCGCACCGTC
    CCCATGCTGC TGGCCAAAGC CTCGTTCCAG GGGGACGTCA AGGACTGGAG CACTCTCATC
    AACCTGCACT GCCACCTCAG CATGGAGGTG AACTACTATA ATGAGATGAT GGGTTTATGG
    GAGCCGCTGT TGGAGCCGCT GGACGATGAG GATAAAGACG GTTTTAGACC CTGGACTCTC
    GCACTGAAGA TGAAGAAGAA GCCGGTGGTC AGTTCTGGGG TGTCTGATGA GGTGGACGGC
    GCTGTGCCTG ATTATAAAAC GGTCATCAGC TTCACTAGTA AAGACCAGCT TAACGTCACC
    CTGTCCAAGT GTGGTCTGCA CATGCTCAGT AATCTGGGAA CGGCTTTTGC AGAGGCCGCT
    AAACAGACGG CCGAGTGCTT TCAGACAGAT CAGACACCAT TCGTGGTGAA GAATCGCCTG
    GGTTTGGCGG TGTCTGTGCT GCACAGCGAA GTGTTTCGAC CCATCGGGAA GGAGTCTAAG
    GATCGTGTGG TGGAGCTCCC GGATGGAGAG AGTCTGAATT TGGACTATAC CCTCACCGCC
    GAGTCCAGCG ACCAGTTCTC TGCCATGACC AGCCTCAGTG CCAAAAACTA CATACAGCCC
    ACTCCAGTCG GCCACACGAC CGCAAGTGTG ATCCCGCTCA TCAAAACGGG CCGGGGCATG
    TACAGCGTCA TGCACAACGA GTCCCGCGTC ACTCGCTTCT TCGTCTGTCA GATTTACACC
    GTCGAGGGCA GCAAATATGT CAAGATTCGC TCTCCCTTCC AGATTATCAA CCACTTTTCA
    GTGCCGTTCC GTGTTTATGA AGGATCCGTG TTTCTGGGCG AGTCTCTGCC GACAGAGGAG
    TTCTGTGTGC CGCTGGGATC CTACAGAGCT GAGCTGAGTC TGCAGCCTGT GCCTGATGGC
    GGTGAAGAGT ACGAGCACTC CGAGAGCTTC ACCTACACTG AGGTTGTCCA GAAACCCGGA
    AAGTGTCTGG AGAAAAACTG CCATCTGCGT GGTGACAGCG CCCAGATTCT GAAGGTCAAC
    ATGGTTCCTC TTGCGGACAC AGTGACGTCC CACAGCAGCG AGGAGGAGTT CGATAAGCCC
    ATCGTACTGC ATTTGTGGCC CACCGTCCTG CTGCGCAACC TGCTGCCCTA CCCAATTACT
    TTCAAGCTAA AGGGCATCGG AGATTCCCCC TCGGTGTCGC TGAAGGAGGG CCACTCATCC
    CAGATCCACA CCGCCATCAT GGACTTCTCC GTGTTGGATC TGAGGCTGCT GGGATACTTG
    CAGCAGGACT GGGAGTCTCA GTACAGCCTC AGCAGTGAAC AAGACGAGAT CAGCTTCATT
    AAGTTTCGCT CGCTCGCACG TGAGGAGGAG ACTGAGAGCC GGCCGGAGCT GGACATCGCT
    ATGCATGTGA AGAAGGAGGA GGGTCAGGTT GTGCTGGCCG TACACTCGCC GTACTGGATG
    GTGAATAAAA CAAGCCGACT GCTGCAGTAC AAGGCGGATG ATATTCATCG CAAACACCCC
    AAGGATTACG ACATGCCGCT GCTCTTCTCC TTCAAGCCAC GCAACTTCCT GCAAAACAAC
    AAGATCCGCC TGATGATCTC AGAAAGTGAG CTGTCAGATG ATTTCTCCAT CGACACTGTC
    GGGAGTTACG GAGATGTCAA ATGCAAAGGC AGACAGAAGG ATTACTTGGT GGGAGTGAAG
    ATCGATGCTA GCAGCTTCAG CTTGACACGT TTGGTGACTT TTCTGCCATT CTACATGCTG
    GTGAACAGAA CCAAACATGT GATTCGGGTC TGTGAGGAGG GGCAGGAGAA CTGGACAGAA
    GCCCGACCCG GACAGGCTGC TGTTCCATTC TGGCCAGAAA GAGACTCAAA GAAGCTGAGA
    GTGAAGGTGG AGGGCAGTCA GTCGTCACCA CGCGCCTTTG ACTTCCACCA GCCAGAGAAC
    TGCCTCCTGC TGCATCTGGA CAAAAGCTTG GGTGGCATCA TTGTGGATGT GAATCTGACA
    GAGCATTCAG CTGTGATCCT CTTCTCTGAT TATCATGATG GAGCCGCTCC CTTCCTCCTC
    ATCAACCACA CTAAGGACGA GACGCTGCAG TACCACCAGA GCAACAGTAA GAGGAAGCCT
    TCGACGTGGT TTCAGGATGA AATACTGGAT TTATCAATGA GGGATGAGAC TGATTCCCTC
    TGCTCTCAGG CCGAGATCGA TGAACTGAGT CCAGGTCAGG CGGTTTACTA CACCTGGGCT
    GAACCAACCG GCTCCAGGAT CCTCAGCTGG AAACTGGGCA AATACAAAGG AGAGCTCAAG
    AGTGAAGAGG ATCAGTTGCT GGATGTGGAC AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT
    TATGAAGGCC TCCAGCGTGT GGCGCTCTTC ACCAGCGAGC AGCGCATCTT CAAGAAGATC
    TGCGACAGTG AGAAGATAGA GCTGGCTCAG CAAGAAATCA GCATCTTGCT GCAGAACATG
    GGCATTTCGC TGGTCAACAA CAACTCCAGC CAGGAAGTGG CCTTCATTGG CATCACCAGC
    TCTGATGTTG TGTGGGAAAC CAAGCCGAAG AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT
    AAAGAGGCAG CAGTTCTGGA GCAGCACTAC AGAGACTACA TGGAGAACGG CCCAGTGGAC
    AGCACCATCA TAGACCTGGA CAACGACTAC CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC
    ATGAGGATCA TGCAGCCCTA CGATGCTCCA CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG
    GTGGAGTACA GCGTTTCAGC CCGCCAGAAA ACATACAGAG TCCAGATCAA CAGGATCCAG
    ATCCAAAACC AGCTGCAAGG AGCCATATTC CCGTTTGTGT TTTACCCCAT CAAACCGCCA
    AAGTCTGTCA GCATGGACTC AGAGCCAAAA CCGCTGGCTG ACGTGAGCAT CATCACCAGA
    GCAGCAGGAC ACTCCGACAT TCTGCGCATC AAATATTTTA TGGTGTTGAT TCAGGAGATG
    GACCTGCGGC TGGATCTGGG CTTTCTGTAT GCCATACTTG AGCTCTTCAC TGCTGAACAC
    ACCAGTGCTG TGACGTCTGA ACAGGAGGTG GAGTTGTTTG ATAAAGACAT AGAGTATCTA
    AAGACTGAGT TAAATCATGT TTCAGCTACA GACACATCTC CCATCAGCCT CTACGAGTAC
    TTCCACATCT CTCCTATAAA GCTTCACTTG AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC
    AGCAATAAGC AAGAGCGCGA GACGGAGCTG ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC
    AAGAGCATCG GGGCTACACT TACAGACGCA CAGGACGTCC TCTTTAAACT GGGCTTCTTC
    GAGCTGAGGT ATCAGTTTCG CACCACTCAG CAGCTGCAGT GGGAGGTCGT TCGCCATTAC
    TCCAAACAGG CTATCAAGCA GATGTATGTC CTTGTTCTGG GTTTGGACGT TCTTGGGAAT
    CCATTCGGGC TGATCCGGGG CCTGTCTGAA GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT
    CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT
    CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC GGAGCGGCGT CTCGCATCAC TGGAGCGATG
    GCTAAAGGTG TGGCGGCGAT GACGATGGAT GAAGAGTACC AGCAGAAGAG ACGAGAGGCC
    ATGAACAAAC AGCCCAGCGG ACTGAGAGAA GGACTGACAC GAGGAGGAAA AGGACTGGTG
    TCTGGCTTCG TGAGTGGCAT CACGGGCATT GTGACCAAAC CCATTAAAGG AGCTCAGAAG
    GAAGGAGCGG CAGGTTTCTT CAAGGGTATG GGTAAGGGTT TAGTTGGAGC TCTGACCAGA
    CCAACAGGGG GCATCATTGA CATGGCCAGC AGCACCTTCC AGGGCATCAA GAGAGCTGCA
    GAGCTGTCTC AGGATGTGGA GAGTCTGCGC CCTCCACGCT TCATCCACGA GGACGGAGTG
    ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC GGCAGTCAGA TGCTGCAGAG TATTGAAAAC
    GGACGGTTTG CCAAGTACAG GTACTTTGCT CACGCTAAAG TCAACGAGTC TGATTTCCTG
    ATGATCACTA AAAGGGGGAT CTTTTTCGTG ACAAAGGGCA CGTTTGGTCA GTTGACATGT
    GAATGGCAGT ATCTGTTCGA TGAGTTTACT AAAGCACCCA TGATTGTGGA AGGCAGGAGA
    CTGCGCATCG AAGCGAAGGA GAGAGTCAAG TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG
    ATCATCAATT TCAAAACTCC AGAATTAGCA AAGTGGGTTC TTGCCAAGCT TGAAGACGCT
    CGGGAGAATC TGCCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa34180
    Clone ID Related Accession (Same CDS sequence) NM_001118893.2
    Accession Version NM_001118893.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-07
    Organism Danio rerio(zebrafish)
    Product vacuolar protein sorting-associated protein 13A
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005248.13. On Jul 23, 2009 this sequence version replaced NM_001118893.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTT TTGAGGATGT GGTGAATTTG CACATCCGCG CAGACCTCAA CTGTCTGAAG
    GTGTTCATAC GAGGAGAGAA AGCCAGGATC TCTGAGATCA GCATAGAGGG ACTGGTTTCT
    GAAGTTCAGA TGAAGAAGAA GTCTGTGGAG ATTCTGGCTA ACTTGAAGAA CATCATCATT
    CTGGATTGTG ACAAAGAGGC ACTTTATAAA AAGGCAGTGT CCATCGCTGA TAAGGAGGTG
    TTTGATTTCC GCATGGTGAA CCATGTCGAT GCAACGGAGG GTGACGCTTA CACAGACATG
    CACATTGTGG ACACTAGCGT AACCCTGACC GTGGGCTGCA TTCAAGTTAT TTTCCTCAAC
    AAGTTTATGT CCTCAATACT GGCATTTATT AATAATTTCC AGGAGGCTAA AAATGCATTG
    GCGGAGGTGA CGGCCCAGGC AGCAGAGAAA GCAGCCACGG GGGTTCTGGA GCTGGCAGAG
    AGAAGTACTC GGATCTCCCT GGACGTCGAC ATCAAAGCTC CTGTTGTCTT CCTCCCACAA
    TCCTCAGTTT CCCACAACGT CATTGTGGCA GATCTGGGTT TAGTGACTGT AAAGAACCGC
    TTTGAGATCG TCTCCTCTCC AACACACACC AAGATCCCTC CTGTCATGGA CATCATGGCT
    GTCGGATTGA GTGATCTGAA AATGTACAGG ACAACGTTCA CTGATGACAA GTTTAACAGC
    GAGATTCAGT TGCTCAAGCC AGTCAACATG GACCTGTCAA TCAAACGCAA TCTCTCCGCC
    TCCTGGTATC ACAGCATACC CGACATCCAG ATCAATGCTC ACCTCAAGCC CATGAGCCTG
    ATCTTGAGTC AGGAGGATCT GACTGTGGTG CTGAAGACTC TGAGTGAGAA TCTGTCCGAG
    ACGTCTGATG CTCCTCCAGC TCCGTCCAGC TCTGATAAAG GAGATACGGT TTCTAACAAA
    GGCTCCCAGG GTTCTGGAGG CAACACCGTT GTGACCGCAG CAGTGGTGGA GGGTCAGAAA
    TCAGGCAAAC TGAAGAACAC CCTAAAACTG AACTTCAAGT TTGACTCCAT GACGCTGGTC
    CTCTACAGCC CGAACAGCGA GCAAGCGAAA TTGTCCGACT CTCGTGATGA GAGTTTGAAG
    TTGGCCGAGT TCACACTGGG CACCATCTCC ACCAGTGTGC ACATGTTCTC CGACAGCTCC
    ATGAAGGCCT CGCTCAAACT CACCAACTGT CTGCTGGATG ACAAGAGGCA GGGAGTGAAG
    ATGATTACAT CCAGGATGAT GGCGATGCGT CAGGACCGAG AGGACAGTGT GATGGTGGAC
    GTCAGCTACC GCCAGAGTCG GGACGGGACT CGTCTGGACA CCCTCGTGCA AGACCTTTAT
    CTTTGTGCCA GTATGGAGTT TCTGCTCACT GTAGCTGATG TGTTTCTTAA AGCTCTGTCT
    GACGGCTTCT CCGGTCCTCC AAAGATTGCT CTGTGGCTGC TGAAATGCTT GGCCCCCGAT
    AAATTGGTTG CTAAAACCGA GATGAATGTG GTGGTGAAGA ACCCAGAGAT CGTGTTTGTG
    GCGGATCTGA CGCGTGCTGA AGCTCCGGCT CTGGTCATGA CCACTCAGTG TGAGCTGGTG
    ATGAAGAGCG AGCCCAGCGG TCAGTGCATG ACGGCCGCCA TTTCAGACCT GAAGATTGTG
    GCCTGTCCGT TCCTGCGGGA GAAGAGGGAG AACAACGTGA CGACGGTCCT ACAGCCCTGC
    GAGGTTTTCT TCCAGAGCCG ACAGTCAAAC ACTGAGCCGC AGTGCATCGA GCTGTCCATC
    AGCGCACTCA CGCTCAAGGT GTCTCCGATC ATCATAAACA CGGTCATCAC CATTCAGACG
    GCTCTAAGTC CTGCCGCTGA GACGCCGCAG GAGCTGGAGA GCCCCATCCC ACAGGACCTA
    TGGGAGCGCC GCAGCCTTCG CGACCTGAAG CTCTGGTTCC TGGAGGAGGA GGGCGAGGAG
    GTCAGCACGT CCGTCACGCG TCTCATTCCT CAGGGAGAGT CTCTGAAGAT GAGCATCGCC
    TCCATCTGCC TGACGCTGGA GGCCGGAGTG GGACACCGCA CCGTCCCCAT GCTGCTGGCC
    AAAGCCTCGT TCCAGGGGGA CGTCAAGGAC TGGAGCACTC TCATCAACCT GCACTGCCAC
    CTCAGCATGG AGGTGAACTA CTATAATGAG ATGATGGGTT TATGGGAGCC GCTGTTGGAG
    CCGCTGGACG ATGAGGATAA AGACGGTTTT AGACCCTGGA CTCTCGCACT GAAGATGAAG
    AAGAAGCCGG TGGTCAGTTC TGGGGTGTCT GATGAGGTGG ACGGCGCTGT GCCTGATTAT
    AAAACGGTCA TCAGCTTCAC TAGTAAAGAC CAGCTTAACG TCACCCTGTC CAAGTGTGGT
    CTGCACATGC TCAGTAATCT GGGAACGGCT TTTGCAGAGG CCGCTAAACA GACGGCCGAG
    TGCTTTCAGA CAGATCAGAC ACCATTCGTG GTGAAGAATC GCCTGGGTTT GGCGGTGTCT
    GTGCTGCACA GCGAAGTGTT TCGACCCATC GGGAAGGAGT CTAAGGATCG TGTGGTGGAG
    CTCCCGGATG GAGAGAGTCT GAATTTGGAC TATACCCTCA CCGCCGAGTC CAGCGACCAG
    TTCTCTGCCA TGACCAGCCT CAGTGCCAAA AACTACATAC AGCCCACTCC AGTCGGCCAC
    ACGACCGCAA GTGTGATCCC GCTCATCAAA ACGGGCCGGG GCATGTACAG CGTCATGCAC
    AACGAGTCCC GCGTCACTCG CTTCTTCGTC TGTCAGATTT ACACCGTCGA GGGCAGCAAA
    TATGTCAAGA TTCGCTCTCC CTTCCAGATT ATCAACCACT TTTCAGTGCC GTTCCGTGTT
    TATGAAGGAT CCGTGTTTCT GGGCGAGTCT CTGCCGACAG AGGAGTTCTG TGTGCCGCTG
    GGATCCTACA GGTCTCTTAC AGCATTTCTG CAGCCTGTGC CTGATGGCGG TGAAGAGTAC
    GAGCACTCCG AGAGCTTCAC CTACACTGAG GTTGTCCAGA AACCCGGAAA GTGTCTGGAG
    AAAAACTGCC ATCTGCGTGG TGACAGCGCC CAGATTCTGA AGGTCAACAT GGTTCCTCTT
    GCGGACACAG TGACGTCCCA CAGCAGCGAG GAGGAGTTCG ATAAGCCCAT CGTACTGCAT
    TTGTGGCCCA CCGTCCTGCT GCGCAACCTG CTGCCCTACC CAATTACTTT CAAGCTAAAG
    GGCATCGGAG ATTCCCCCTC GGTGTCGCTG AAGGAGGGCC ACTCATCCCA GATCCACACC
    GCCATCATGG ACTTCTCCGT GTTGGATCTG AGGCTGCTGG GATACTTGCA GCAGGACTGG
    GAGTCTCAGT ACAGCCTCAG CAGTGAACAA GACGAGATCA GCTTCATTAA GTTTCGCTCG
    CTCGCACGTG AGGAGGAGAC TGAGAGCCGG CCGGAGCTGG ACATCGCTAT GCATGTGAAG
    AAGGAGGAGG GTCAGGTTGT GCTGGCCGTA CACTCGCCGT ACTGGATGGT GAATAAAACA
    AGCCGACTGC TGCAGTACAA GGCGGATGAT ATTCATCGCA AACACCCCAA GGATTACGAC
    ATGCCGCTGC TCTTCTCCTT CAAGCCACGC AACTTCCTGC AAAACAACAA GATCCGCCTG
    ATGATCTCAG AAAGTGAGCT GTCAGATGAT TTCTCCATCG ACACTGTCGG GAGTTACGGA
    GATGTCAAAT GCAAAGGCAG ACAGAAGGAT TACTTGGTGG GAGTGAAGAT CGATGCTAGC
    AGCTTCAGCT TGACACGTTT GGTGACTTTT CTGCCATTCT ACATGCTGGT GAACAGAACC
    AAACATGTGA TTCGGGTCTG TGAGGAGGGG CAGGAGAACT GGTTAAGTTC TTCTGTTCAG
    GCTGCTGTTC CATTCTGGCC AGAAAGAGAC TCAAAGAAGC TGAGAGTGAA GGTGGAGGGC
    AGTCAGTCGT CACCACGCGC CTTTGACTTC CACCAGCCAG AGAACTGCCT CCTGCTGCAT
    CTGGACAAAA GCTTGGGTGG CATCATTGTG GATGTGAATC TGACAGAGCA TTCAGCTGTG
    ATCCTCTTCT CTGATTATCA TGATGGAGCC GCTCCCTTCC TCCTCATCAA CCACACTAAG
    GACGAGACGC TGCAGTACCA CCAGAGCTCT CAGGCCGAGA TCGATGAACT GAGTCCAGGT
    CAGGCGGTTT ACTACACCTG GGCTGAACCA ACCGGCTCCA GGATCCTCAG CTGGAAACTG
    GGCAAATACA AAGGAGAGCT CAAGAGTGAA GAGGATCAGT TGCTGGATGT GGACAAGGAG
    GGCAAGTTGT TTTTGGTGTC GTTTTATGAA GGCCTCCAGC GTGTGGCGCT CTTCACCAGC
    GAGCAGCGCA TCTTCAAGAA GATCTGCGAC AGTGAGAAGA TAGAGCTGGC TCAGCAAGAA
    ATCAGCATCT TGCTGCAGAA CATGGGCATT TCGCTGGTCA ACAACAACTC CAGCCAGGAA
    GTGGCCTTCA TTGGCATCAC CAGCTCTGAT GTTGTGTGGG AAACCAAGCC GAAGAAGCGC
    AGCCGCTGGA AAGTTCTGAG TGTTAAAGAG GCAGCAGTTC TGGAGCAGCA CTACAGAGAC
    TACATGGAGA ACGGCCCAGT GGACAGCACC ATCATAGACC TGGACAACGA CTACCAGGTG
    TTGTTTGCTG CTAACGGTGT GGACATGAGG ATCATGCAGC CCTACGATGC TCCACTCAGA
    AGGAACTTCC TGCCGGCGTT GAAGGTGGAG TACAGCGTTT CAGCCCGCCA GAAAACATAC
    AGAGTCCAGA TCAACAGGAT CCAGATCCAA AACCAGCTGC AAGGAGCCAT ATTCCCGTTT
    GTGTTTTACC CCATCAAACC GCCAAAGTCT GTCAGCATGG ACTCAGAGCC AAAACCGCTG
    GCTGACGTGA GCATCATCAC CAGAGCAGCA GGACACTCCG ACATTCTGCG CATCAAATAT
    TTTATGGTGT TGATTCAGGA GATGGACCTG CGGCTGGATC TGGGCTTTCT GTATGCCATA
    CTTGAGCTCT TCACTGCTGA ACACACCAGT GCTGTGACGT CTGAACAGGA GGTGGAGTTG
    TTTGATAAAG ACATAGAGTA TCTAAAGACT GAGTTAAATC ATGTTTCAGC TACAGACACA
    TCTCCCATCA GCCTCTACGA GTACTTCCAC ATCTCTCCTA TAAAGCTTCA CTTGAGCTTG
    TCTCTGAGCA GTGGTGGTGA AGACAGCAAT AAGCAAGAGC GCGAGACGGA GCTGATCCCA
    GTTCAGTCTC TCCATCTGCT GCTCAAGAGC ATCGGGGCTA CACTTACAGA CGCACAGGAC
    GTCCTCTTTA AACTGGGCTT CTTCGAGCTG AGGTATCAGT TTCGCACCAC TCAGCAGCTG
    CAGTGGGAGG TCGTTCGCCA TTACTCCAAA CAGGCTATCA AGCAGATGTA TGTCCTTGTT
    CTGGGTTTGG ACGTTCTTGG GAATCCATTC GGGCTGATCC GGGGCCTGTC TGAAGGCGTC
    GAGGCTTTCT TTTACGAGCC CTATCAGGGT GCAATTCAGG GTCCAGAGGA GTTCATGGAG
    GGCATGGCTC TCGGCGTGAA GGCTCTGGTT GGAGGCGCTG TGGGTGGTCT GGCCGGAGCG
    GCGTCTCGCA TCACTGGAGC GATGGCTAAA GGTGTGGCGG CGATGACGAT GGATGAAGAG
    TACCAGCAGA AGAGACGAGA GGCCATGAAC AAACAGCCCA GCGGACTGAG AGAAGGACTG
    ACACGAGGAG GAAAAGGACT GGTGTCTGGC TTCGTGAGTG GCATCACGGG CATTGTGACC
    AAACCCATTA AAGGAGCTCA GAAGGAAGGA GCGGCAGGTT TCTTCAAGGG TATGGGTAAG
    GGTTTAGTTG GAGCTCTGAC CAGACCAACA GGGGGCATCA TTGACATGGC CAGCAGCACC
    TTCCAGGGCA TCAAGAGAGC TGCAGAGCTG TCTCAGGATG TGGAGAGTCT GCGCCCTCCA
    CGCTTCATCC ACGAGGACGG AGTGATCCGG CCGTACAGAG AGAGAGAAGG CCTCGGCAGT
    CAGATGCTGC AGAGTATTGA AAACGGACGG TTTGCCAAGT ACAGGTACTT TGCTCACGCT
    AAAGTCAACG AGTCTGATTT CCTGATGATC ACTAAAAGGG GGATCTTTTT CGTGACAAAG
    GGCACGTTTG GTCAGTTGAC ATGTGAATGG CAGTATCTGT TCGATGAGTT TACTAAAGCA
    CCCATGATTG TGGAAGGCAG GAGACTGCGC ATCGAAGCGA AGGAGAGAGT CAAGTCAGTG
    TTTCACGCCA AAGAGTTCGG GAAGATCATC AATTTCAAAA CTCCAGAATT AGCAAAGTGG
    GTTCTTGCCA AGCTTGAAGA CGCTCGGGAG AATCTGCCCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001112365.1
    CDS51..9635
    Translation

    Target ORF information:

    RefSeq Version NM_001118893.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio vacuolar protein sorting 13 homolog A (vps13a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001118893.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTT TTGAGGATGT GGTGAATTTG CACATCCGCG CAGACCTCAA CTGTCTGAAG
    GTGTTCATAC GAGGAGAGAA AGCCAGGATC TCTGAGATCA GCATAGAGGG ACTGGTTTCT
    GAAGTTCAGA TGAAGAAGAA GTCTGTGGAG ATTCTGGCTA ACTTGAAGAA CATCATCATT
    CTGGATTGTG ACAAAGAGGC ACTTTATAAA AAGGCAGTGT CCATCGCTGA TAAGGAGGTG
    TTTGATTTCC GCATGGTGAA CCATGTCGAT GCAACGGAGG GTGACGCTTA CACAGACATG
    CACATTGTGG ACACTAGCGT AACCCTGACC GTGGGCTGCA TTCAAGTTAT TTTCCTCAAC
    AAGTTTATGT CCTCAATACT GGCATTTATT AATAATTTCC AGGAGGCTAA AAATGCATTG
    GCGGAGGTGA CGGCCCAGGC AGCAGAGAAA GCAGCCACGG GGGTTCTGGA GCTGGCAGAG
    AGAAGTACTC GGATCTCCCT GGACGTCGAC ATCAAAGCTC CTGTTGTCTT CCTCCCACAA
    TCCTCAGTTT CCCACAACGT CATTGTGGCA GATCTGGGTT TAGTGACTGT AAAGAACCGC
    TTTGAGATCG TCTCCTCTCC AACACACACC AAGATCCCTC CTGTCATGGA CATCATGGCT
    GTCGGATTGA GTGATCTGAA AATGTACAGG ACAACGTTCA CTGATGACAA GTTTAACAGC
    GAGATTCAGT TGCTCAAGCC AGTCAACATG GACCTGTCAA TCAAACGCAA TCTCTCCGCC
    TCCTGGTATC ACAGCATACC CGACATCCAG ATCAATGCTC ACCTCAAGCC CATGAGCCTG
    ATCTTGAGTC AGGAGGATCT GACTGTGGTG CTGAAGACTC TGAGTGAGAA TCTGTCCGAG
    ACGTCTGATG CTCCTCCAGC TCCGTCCAGC TCTGATAAAG GAGATACGGT TTCTAACAAA
    GGCTCCCAGG GTTCTGGAGG CAACACCGTT GTGACCGCAG CAGTGGTGGA GGGTCAGAAA
    TCAGGCAAAC TGAAGAACAC CCTAAAACTG AACTTCAAGT TTGACTCCAT GACGCTGGTC
    CTCTACAGCC CGAACAGCGA GCAAGCGAAA TTGTCCGACT CTCGTGATGA GAGTTTGAAG
    TTGGCCGAGT TCACACTGGG CACCATCTCC ACCAGTGTGC ACATGTTCTC CGACAGCTCC
    ATGAAGGCCT CGCTCAAACT CACCAACTGT CTGCTGGATG ACAAGAGGCA GGGAGTGAAG
    ATGATTACAT CCAGGATGAT GGCGATGCGT CAGGACCGAG AGGACAGTGT GATGGTGGAC
    GTCAGCTACC GCCAGAGTCG GGACGGGACT CGTCTGGACA CCCTCGTGCA AGACCTTTAT
    CTTTGTGCCA GTATGGAGTT TCTGCTCACT GTAGCTGATG TGTTTCTTAA AGCTCTGTCT
    GACGGCTTCT CCGGTCCTCC AAAGATTGCT CTGTGGCTGC TGAAATGCTT GGCCCCCGAT
    AAATTGGTTG CTAAAACCGA GATGAATGTG GTGGTGAAGA ACCCAGAGAT CGTGTTTGTG
    GCGGATCTGA CGCGTGCTGA AGCTCCGGCT CTGGTCATGA CCACTCAGTG TGAGCTGGTG
    ATGAAGAGCG AGCCCAGCGG TCAGTGCATG ACGGCCGCCA TTTCAGACCT GAAGATTGTG
    GCCTGTCCGT TCCTGCGGGA GAAGAGGGAG AACAACGTGA CGACGGTCCT ACAGCCCTGC
    GAGGTTTTCT TCCAGAGCCG ACAGTCAAAC ACTGAGCCGC AGTGCATCGA GCTGTCCATC
    AGCGCACTCA CGCTCAAGGT GTCTCCGATC ATCATAAACA CGGTCATCAC CATTCAGACG
    GCTCTAAGTC CTGCCGCTGA GACGCCGCAG GAGCTGGAGA GCCCCATCCC ACAGGACCTA
    TGGGAGCGCC GCAGCCTTCG CGACCTGAAG CTCTGGTTCC TGGAGGAGGA GGGCGAGGAG
    GTCAGCACGT CCGTCACGCG TCTCATTCCT CAGGGAGAGT CTCTGAAGAT GAGCATCGCC
    TCCATCTGCC TGACGCTGGA GGCCGGAGTG GGACACCGCA CCGTCCCCAT GCTGCTGGCC
    AAAGCCTCGT TCCAGGGGGA CGTCAAGGAC TGGAGCACTC TCATCAACCT GCACTGCCAC
    CTCAGCATGG AGGTGAACTA CTATAATGAG ATGATGGGTT TATGGGAGCC GCTGTTGGAG
    CCGCTGGACG ATGAGGATAA AGACGGTTTT AGACCCTGGA CTCTCGCACT GAAGATGAAG
    AAGAAGCCGG TGGTCAGTTC TGGGGTGTCT GATGAGGTGG ACGGCGCTGT GCCTGATTAT
    AAAACGGTCA TCAGCTTCAC TAGTAAAGAC CAGCTTAACG TCACCCTGTC CAAGTGTGGT
    CTGCACATGC TCAGTAATCT GGGAACGGCT TTTGCAGAGG CCGCTAAACA GACGGCCGAG
    TGCTTTCAGA CAGATCAGAC ACCATTCGTG GTGAAGAATC GCCTGGGTTT GGCGGTGTCT
    GTGCTGCACA GCGAAGTGTT TCGACCCATC GGGAAGGAGT CTAAGGATCG TGTGGTGGAG
    CTCCCGGATG GAGAGAGTCT GAATTTGGAC TATACCCTCA CCGCCGAGTC CAGCGACCAG
    TTCTCTGCCA TGACCAGCCT CAGTGCCAAA AACTACATAC AGCCCACTCC AGTCGGCCAC
    ACGACCGCAA GTGTGATCCC GCTCATCAAA ACGGGCCGGG GCATGTACAG CGTCATGCAC
    AACGAGTCCC GCGTCACTCG CTTCTTCGTC TGTCAGATTT ACACCGTCGA GGGCAGCAAA
    TATGTCAAGA TTCGCTCTCC CTTCCAGATT ATCAACCACT TTTCAGTGCC GTTCCGTGTT
    TATGAAGGAT CCGTGTTTCT GGGCGAGTCT CTGCCGACAG AGGAGTTCTG TGTGCCGCTG
    GGATCCTACA GGTCTCTTAC AGCATTTCTG CAGCCTGTGC CTGATGGCGG TGAAGAGTAC
    GAGCACTCCG AGAGCTTCAC CTACACTGAG GTTGTCCAGA AACCCGGAAA GTGTCTGGAG
    AAAAACTGCC ATCTGCGTGG TGACAGCGCC CAGATTCTGA AGGTCAACAT GGTTCCTCTT
    GCGGACACAG TGACGTCCCA CAGCAGCGAG GAGGAGTTCG ATAAGCCCAT CGTACTGCAT
    TTGTGGCCCA CCGTCCTGCT GCGCAACCTG CTGCCCTACC CAATTACTTT CAAGCTAAAG
    GGCATCGGAG ATTCCCCCTC GGTGTCGCTG AAGGAGGGCC ACTCATCCCA GATCCACACC
    GCCATCATGG ACTTCTCCGT GTTGGATCTG AGGCTGCTGG GATACTTGCA GCAGGACTGG
    GAGTCTCAGT ACAGCCTCAG CAGTGAACAA GACGAGATCA GCTTCATTAA GTTTCGCTCG
    CTCGCACGTG AGGAGGAGAC TGAGAGCCGG CCGGAGCTGG ACATCGCTAT GCATGTGAAG
    AAGGAGGAGG GTCAGGTTGT GCTGGCCGTA CACTCGCCGT ACTGGATGGT GAATAAAACA
    AGCCGACTGC TGCAGTACAA GGCGGATGAT ATTCATCGCA AACACCCCAA GGATTACGAC
    ATGCCGCTGC TCTTCTCCTT CAAGCCACGC AACTTCCTGC AAAACAACAA GATCCGCCTG
    ATGATCTCAG AAAGTGAGCT GTCAGATGAT TTCTCCATCG ACACTGTCGG GAGTTACGGA
    GATGTCAAAT GCAAAGGCAG ACAGAAGGAT TACTTGGTGG GAGTGAAGAT CGATGCTAGC
    AGCTTCAGCT TGACACGTTT GGTGACTTTT CTGCCATTCT ACATGCTGGT GAACAGAACC
    AAACATGTGA TTCGGGTCTG TGAGGAGGGG CAGGAGAACT GGTTAAGTTC TTCTGTTCAG
    GCTGCTGTTC CATTCTGGCC AGAAAGAGAC TCAAAGAAGC TGAGAGTGAA GGTGGAGGGC
    AGTCAGTCGT CACCACGCGC CTTTGACTTC CACCAGCCAG AGAACTGCCT CCTGCTGCAT
    CTGGACAAAA GCTTGGGTGG CATCATTGTG GATGTGAATC TGACAGAGCA TTCAGCTGTG
    ATCCTCTTCT CTGATTATCA TGATGGAGCC GCTCCCTTCC TCCTCATCAA CCACACTAAG
    GACGAGACGC TGCAGTACCA CCAGAGCTCT CAGGCCGAGA TCGATGAACT GAGTCCAGGT
    CAGGCGGTTT ACTACACCTG GGCTGAACCA ACCGGCTCCA GGATCCTCAG CTGGAAACTG
    GGCAAATACA AAGGAGAGCT CAAGAGTGAA GAGGATCAGT TGCTGGATGT GGACAAGGAG
    GGCAAGTTGT TTTTGGTGTC GTTTTATGAA GGCCTCCAGC GTGTGGCGCT CTTCACCAGC
    GAGCAGCGCA TCTTCAAGAA GATCTGCGAC AGTGAGAAGA TAGAGCTGGC TCAGCAAGAA
    ATCAGCATCT TGCTGCAGAA CATGGGCATT TCGCTGGTCA ACAACAACTC CAGCCAGGAA
    GTGGCCTTCA TTGGCATCAC CAGCTCTGAT GTTGTGTGGG AAACCAAGCC GAAGAAGCGC
    AGCCGCTGGA AAGTTCTGAG TGTTAAAGAG GCAGCAGTTC TGGAGCAGCA CTACAGAGAC
    TACATGGAGA ACGGCCCAGT GGACAGCACC ATCATAGACC TGGACAACGA CTACCAGGTG
    TTGTTTGCTG CTAACGGTGT GGACATGAGG ATCATGCAGC CCTACGATGC TCCACTCAGA
    AGGAACTTCC TGCCGGCGTT GAAGGTGGAG TACAGCGTTT CAGCCCGCCA GAAAACATAC
    AGAGTCCAGA TCAACAGGAT CCAGATCCAA AACCAGCTGC AAGGAGCCAT ATTCCCGTTT
    GTGTTTTACC CCATCAAACC GCCAAAGTCT GTCAGCATGG ACTCAGAGCC AAAACCGCTG
    GCTGACGTGA GCATCATCAC CAGAGCAGCA GGACACTCCG ACATTCTGCG CATCAAATAT
    TTTATGGTGT TGATTCAGGA GATGGACCTG CGGCTGGATC TGGGCTTTCT GTATGCCATA
    CTTGAGCTCT TCACTGCTGA ACACACCAGT GCTGTGACGT CTGAACAGGA GGTGGAGTTG
    TTTGATAAAG ACATAGAGTA TCTAAAGACT GAGTTAAATC ATGTTTCAGC TACAGACACA
    TCTCCCATCA GCCTCTACGA GTACTTCCAC ATCTCTCCTA TAAAGCTTCA CTTGAGCTTG
    TCTCTGAGCA GTGGTGGTGA AGACAGCAAT AAGCAAGAGC GCGAGACGGA GCTGATCCCA
    GTTCAGTCTC TCCATCTGCT GCTCAAGAGC ATCGGGGCTA CACTTACAGA CGCACAGGAC
    GTCCTCTTTA AACTGGGCTT CTTCGAGCTG AGGTATCAGT TTCGCACCAC TCAGCAGCTG
    CAGTGGGAGG TCGTTCGCCA TTACTCCAAA CAGGCTATCA AGCAGATGTA TGTCCTTGTT
    CTGGGTTTGG ACGTTCTTGG GAATCCATTC GGGCTGATCC GGGGCCTGTC TGAAGGCGTC
    GAGGCTTTCT TTTACGAGCC CTATCAGGGT GCAATTCAGG GTCCAGAGGA GTTCATGGAG
    GGCATGGCTC TCGGCGTGAA GGCTCTGGTT GGAGGCGCTG TGGGTGGTCT GGCCGGAGCG
    GCGTCTCGCA TCACTGGAGC GATGGCTAAA GGTGTGGCGG CGATGACGAT GGATGAAGAG
    TACCAGCAGA AGAGACGAGA GGCCATGAAC AAACAGCCCA GCGGACTGAG AGAAGGACTG
    ACACGAGGAG GAAAAGGACT GGTGTCTGGC TTCGTGAGTG GCATCACGGG CATTGTGACC
    AAACCCATTA AAGGAGCTCA GAAGGAAGGA GCGGCAGGTT TCTTCAAGGG TATGGGTAAG
    GGTTTAGTTG GAGCTCTGAC CAGACCAACA GGGGGCATCA TTGACATGGC CAGCAGCACC
    TTCCAGGGCA TCAAGAGAGC TGCAGAGCTG TCTCAGGATG TGGAGAGTCT GCGCCCTCCA
    CGCTTCATCC ACGAGGACGG AGTGATCCGG CCGTACAGAG AGAGAGAAGG CCTCGGCAGT
    CAGATGCTGC AGAGTATTGA AAACGGACGG TTTGCCAAGT ACAGGTACTT TGCTCACGCT
    AAAGTCAACG AGTCTGATTT CCTGATGATC ACTAAAAGGG GGATCTTTTT CGTGACAAAG
    GGCACGTTTG GTCAGTTGAC ATGTGAATGG CAGTATCTGT TCGATGAGTT TACTAAAGCA
    CCCATGATTG TGGAAGGCAG GAGACTGCGC ATCGAAGCGA AGGAGAGAGT CAAGTCAGTG
    TTTCACGCCA AAGAGTTCGG GAAGATCATC AATTTCAAAA CTCCAGAATT AGCAAAGTGG
    GTTCTTGCCA AGCTTGAAGA CGCTCGGGAG AATCTGCCCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa14675
    Clone ID Related Accession (Same CDS sequence) XM_005167334.4 , XM_005167334.3
    Accession Version XM_005167334.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product vacuolar protein sorting-associated protein 13A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167334.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT
    TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT
    CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG
    AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC
    AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC
    ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG
    CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC
    CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG
    AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC
    AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC
    CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA
    CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA
    ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC
    CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA
    CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC
    AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT
    GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG
    GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA
    GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG
    AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG
    ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA
    CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG
    CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC
    CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA
    GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC
    ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC
    GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT
    GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA
    GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT
    GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG
    GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC
    AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC
    CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC
    GGCAGTCAGA TGCTGCAGAG TATTGAAAAC GGACGGTTTG CCAAGTACAG GTACTTTGCT
    CACGCTAAAG TCAACGAGTC TGATTTCCTG ATGATCACTA AAAGGGGGAT CTTTTTCGTG
    ACAAAGGGCA CGTTTGGTCA GTTGACATGT GAATGGCAGT ATCTGTTCGA TGAGTTTACT
    AAAGCACCCA TGATTGTGGA AGGCAGGAGA CTGCGCATCG AAGCGAAGGA GAGAGTCAAG
    TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG ATCATCAATT TCAAAACTCC AGAATTAGCA
    AAGTGGGTTC TTGCCAAGCT TGAAGACGCT CGGGAGAATC TGCCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167391.1
    CDS153..9863
    Translation

    Target ORF information:

    RefSeq Version XM_005167334.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167334.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT
    TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT
    CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG
    AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC
    AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC
    ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG
    CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC
    CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG
    AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC
    AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC
    CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA
    CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA
    ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC
    CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA
    CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC
    AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT
    GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG
    GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA
    GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG
    AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG
    ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA
    CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG
    CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC
    CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA
    GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC
    ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC
    GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT
    GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA
    GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT
    GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG
    GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC
    AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC
    CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC
    GGCAGTCAGA TGCTGCAGAG TATTGAAAAC GGACGGTTTG CCAAGTACAG GTACTTTGCT
    CACGCTAAAG TCAACGAGTC TGATTTCCTG ATGATCACTA AAAGGGGGAT CTTTTTCGTG
    ACAAAGGGCA CGTTTGGTCA GTTGACATGT GAATGGCAGT ATCTGTTCGA TGAGTTTACT
    AAAGCACCCA TGATTGTGGA AGGCAGGAGA CTGCGCATCG AAGCGAAGGA GAGAGTCAAG
    TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG ATCATCAATT TCAAAACTCC AGAATTAGCA
    AAGTGGGTTC TTGCCAAGCT TGAAGACGCT CGGGAGAATC TGCCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa14675
    Clone ID Related Accession (Same CDS sequence) XM_005167334.4 , XM_005167334.3
    Accession Version XM_005167334.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product vacuolar protein sorting-associated protein 13A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167334.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT
    TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT
    CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG
    AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC
    AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC
    ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG
    CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC
    CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG
    AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC
    AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC
    CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA
    CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA
    ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC
    CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA
    CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC
    AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT
    GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG
    GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA
    GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG
    AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG
    ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA
    CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG
    CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC
    CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA
    GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC
    ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC
    GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT
    GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA
    GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT
    GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG
    GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC
    AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC
    CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC
    GGCAGTCAGA TGCTGCAGAG TATTGAAAAC GGACGGTTTG CCAAGTACAG GTACTTTGCT
    CACGCTAAAG TCAACGAGTC TGATTTCCTG ATGATCACTA AAAGGGGGAT CTTTTTCGTG
    ACAAAGGGCA CGTTTGGTCA GTTGACATGT GAATGGCAGT ATCTGTTCGA TGAGTTTACT
    AAAGCACCCA TGATTGTGGA AGGCAGGAGA CTGCGCATCG AAGCGAAGGA GAGAGTCAAG
    TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG ATCATCAATT TCAAAACTCC AGAATTAGCA
    AAGTGGGTTC TTGCCAAGCT TGAAGACGCT CGGGAGAATC TGCCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167391.1
    CDS152..9862
    Translation

    Target ORF information:

    RefSeq Version XM_005167334.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167334.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT
    TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT
    CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG
    AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC
    AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC
    ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG
    CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC
    CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG
    AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC
    AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC
    CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA
    CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA
    ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC
    CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA
    CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC
    AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT
    GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG
    GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA
    GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG
    AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG
    ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA
    CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG
    CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC
    CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA
    GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC
    ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC
    GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT
    GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA
    GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT
    GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG
    GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC
    AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC
    CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC
    GGCAGTCAGA TGCTGCAGAG TATTGAAAAC GGACGGTTTG CCAAGTACAG GTACTTTGCT
    CACGCTAAAG TCAACGAGTC TGATTTCCTG ATGATCACTA AAAGGGGGAT CTTTTTCGTG
    ACAAAGGGCA CGTTTGGTCA GTTGACATGT GAATGGCAGT ATCTGTTCGA TGAGTTTACT
    AAAGCACCCA TGATTGTGGA AGGCAGGAGA CTGCGCATCG AAGCGAAGGA GAGAGTCAAG
    TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG ATCATCAATT TCAAAACTCC AGAATTAGCA
    AAGTGGGTTC TTGCCAAGCT TGAAGACGCT CGGGAGAATC TGCCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa40321
    Clone ID Related Accession (Same CDS sequence) XM_009304309.3 , XM_009304309.2
    Accession Version XM_009304309.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product vacuolar protein sorting-associated protein 13A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304309.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCTCTCAGGC CGAGATCGAT GAACTGAGTC CAGGTCAGGC GGTTTACTAC
    ACCTGGGCTG AACCAACCGG CTCCAGGATC CTCAGCTGGA AACTGGGCAA ATACAAAGGA
    GAGCTCAAGA GTGAAGAGGA TCAGTTGCTG GATGTGGACA AGGAGGGCAA GTTGTTTTTG
    GTGTCGTTTT ATGAAGGCCT CCAGCGTGTG GCGCTCTTCA CCAGCGAGCA GCGCATCTTC
    AAGAAGATCT GCGACAGTGA GAAGATAGAG CTGGCTCAGC AAGAAATCAG CATCTTGCTG
    CAGAACATGG GCATTTCGCT GGTCAACAAC AACTCCAGCC AGGAAGTGGC CTTCATTGGC
    ATCACCAGCT CTGATGTTGT GTGGGAAACC AAGCCGAAGA AGCGCAGCCG CTGGAAAGTT
    CTGAGTGTTA AAGAGGCAGC AGTTCTGGAG CAGCACTACA GAGACTACAT GGAGAACGGC
    CCAGTGGACA GCACCATCAT AGACCTGGAC AACGACTACC AGGTGTTGTT TGCTGCTAAC
    GGTGTGGACA TGAGGATCAT GCAGCCCTAC GATGCTCCAC TCAGAAGGAA CTTCCTGCCG
    GCGTTGAAGG TGGAGTACAG CGTTTCAGCC CGCCAGAAAA CATACAGAGT CCAGATCAAC
    AGGATCCAGA TCCAAAACCA GCTGCAAGGA GCCATATTCC CGTTTGTGTT TTACCCCATC
    AAACCGCCAA AGTCTGTCAG CATGGACTCA GAGCCAAAAC CGCTGGCTGA CGTGAGCATC
    ATCACCAGAG CAGCAGGACA CTCCGACATT CTGCGCATCA AATATTTTAT GGTGTTGATT
    CAGGAGATGG ACCTGCGGCT GGATCTGGGC TTTCTGTATG CCATACTTGA GCTCTTCACT
    GCTGAACACA CCAGTGCTGT GACGTCTGAA CAGGAGGTGG AGTTGTTTGA TAAAGACATA
    GAGTATCTAA AGACTGAGTT AAATCATGTT TCAGCTACAG ACACATCTCC CATCAGCCTC
    TACGAGTACT TCCACATCTC TCCTATAAAG CTTCACTTGA GCTTGTCTCT GAGCAGTGGT
    GGTGAAGACA GCAATAAGCA AGAGCGCGAG ACGGAGCTGA TCCCAGTTCA GTCTCTCCAT
    CTGCTGCTCA AGAGCATCGG GGCTACACTT ACAGACGCAC AGGACGTCCT CTTTAAACTG
    GGCTTCTTCG AGCTGAGGTA TCAGTTTCGC ACCACTCAGC AGCTGCAGTG GGAGGTCGTT
    CGCCATTACT CCAAACAGGC TATCAAGCAG ATGTATGTCC TTGTTCTGGG TTTGGACGTT
    CTTGGGAATC CATTCGGGCT GATCCGGGGC CTGTCTGAAG GCGTCGAGGC TTTCTTTTAC
    GAGCCCTATC AGGGTGCAAT TCAGGGTCCA GAGGAGTTCA TGGAGGGCAT GGCTCTCGGC
    GTGAAGGCTC TGGTTGGAGG CGCTGTGGGT GGTCTGGCCG GAGCGGCGTC TCGCATCACT
    GGAGCGATGG CTAAAGGTGT GGCGGCGATG ACGATGGATG AAGAGTACCA GCAGAAGAGA
    CGAGAGGCCA TGAACAAACA GCCCAGCGGA CTGAGAGAAG GACTGACACG AGGAGGAAAA
    GGACTGGTGT CTGGCTTCGT GAGTGGCATC ACGGGCATTG TGACCAAACC CATTAAAGGA
    GCTCAGAAGG AAGGAGCGGC AGGTTTCTTC AAGGGTATGG GTAAGGGTTT AGTTGGAGCT
    CTGACCAGAC CAACAGGGGG CATCATTGAC ATGGCCAGCA GCACCTTCCA GGGCATCAAG
    AGAGCTGCAG AGCTGTCTCA GGATGTGGAG AGTCTGCGCC CTCCACGCTT CATCCACGAG
    GACGGAGTGA TCCGGCCGTA CAGAGAGAGA GAAGGCCTCG GCAGTCAGAT GCTGCAGAGT
    ATTGAAAACG GACGGTTTGC CAAGTACAGG TACTTTGCTC ACGCTAAAGT CAACGAGTCT
    GATTTCCTGA TGATCACTAA AAGGGGGATC TTTTTCGTGA CAAAGGGCAC GTTTGGTCAG
    TTGACATGTG AATGGCAGTA TCTGTTCGAT GAGTTTACTA AAGCACCCAT GATTGTGGAA
    GGCAGGAGAC TGCGCATCGA AGCGAAGGAG AGAGTCAAGT CAGTGTTTCA CGCCAAAGAG
    TTCGGGAAGA TCATCAATTT CAAAACTCCA GAATTAGCAA AGTGGGTTCT TGCCAAGCTT
    GAAGACGCTC GGGAGAATCT GCCCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302584.1
    CDS51..9680
    Translation

    Target ORF information:

    RefSeq Version XM_009304309.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304309.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCTCTCAGGC CGAGATCGAT GAACTGAGTC CAGGTCAGGC GGTTTACTAC
    ACCTGGGCTG AACCAACCGG CTCCAGGATC CTCAGCTGGA AACTGGGCAA ATACAAAGGA
    GAGCTCAAGA GTGAAGAGGA TCAGTTGCTG GATGTGGACA AGGAGGGCAA GTTGTTTTTG
    GTGTCGTTTT ATGAAGGCCT CCAGCGTGTG GCGCTCTTCA CCAGCGAGCA GCGCATCTTC
    AAGAAGATCT GCGACAGTGA GAAGATAGAG CTGGCTCAGC AAGAAATCAG CATCTTGCTG
    CAGAACATGG GCATTTCGCT GGTCAACAAC AACTCCAGCC AGGAAGTGGC CTTCATTGGC
    ATCACCAGCT CTGATGTTGT GTGGGAAACC AAGCCGAAGA AGCGCAGCCG CTGGAAAGTT
    CTGAGTGTTA AAGAGGCAGC AGTTCTGGAG CAGCACTACA GAGACTACAT GGAGAACGGC
    CCAGTGGACA GCACCATCAT AGACCTGGAC AACGACTACC AGGTGTTGTT TGCTGCTAAC
    GGTGTGGACA TGAGGATCAT GCAGCCCTAC GATGCTCCAC TCAGAAGGAA CTTCCTGCCG
    GCGTTGAAGG TGGAGTACAG CGTTTCAGCC CGCCAGAAAA CATACAGAGT CCAGATCAAC
    AGGATCCAGA TCCAAAACCA GCTGCAAGGA GCCATATTCC CGTTTGTGTT TTACCCCATC
    AAACCGCCAA AGTCTGTCAG CATGGACTCA GAGCCAAAAC CGCTGGCTGA CGTGAGCATC
    ATCACCAGAG CAGCAGGACA CTCCGACATT CTGCGCATCA AATATTTTAT GGTGTTGATT
    CAGGAGATGG ACCTGCGGCT GGATCTGGGC TTTCTGTATG CCATACTTGA GCTCTTCACT
    GCTGAACACA CCAGTGCTGT GACGTCTGAA CAGGAGGTGG AGTTGTTTGA TAAAGACATA
    GAGTATCTAA AGACTGAGTT AAATCATGTT TCAGCTACAG ACACATCTCC CATCAGCCTC
    TACGAGTACT TCCACATCTC TCCTATAAAG CTTCACTTGA GCTTGTCTCT GAGCAGTGGT
    GGTGAAGACA GCAATAAGCA AGAGCGCGAG ACGGAGCTGA TCCCAGTTCA GTCTCTCCAT
    CTGCTGCTCA AGAGCATCGG GGCTACACTT ACAGACGCAC AGGACGTCCT CTTTAAACTG
    GGCTTCTTCG AGCTGAGGTA TCAGTTTCGC ACCACTCAGC AGCTGCAGTG GGAGGTCGTT
    CGCCATTACT CCAAACAGGC TATCAAGCAG ATGTATGTCC TTGTTCTGGG TTTGGACGTT
    CTTGGGAATC CATTCGGGCT GATCCGGGGC CTGTCTGAAG GCGTCGAGGC TTTCTTTTAC
    GAGCCCTATC AGGGTGCAAT TCAGGGTCCA GAGGAGTTCA TGGAGGGCAT GGCTCTCGGC
    GTGAAGGCTC TGGTTGGAGG CGCTGTGGGT GGTCTGGCCG GAGCGGCGTC TCGCATCACT
    GGAGCGATGG CTAAAGGTGT GGCGGCGATG ACGATGGATG AAGAGTACCA GCAGAAGAGA
    CGAGAGGCCA TGAACAAACA GCCCAGCGGA CTGAGAGAAG GACTGACACG AGGAGGAAAA
    GGACTGGTGT CTGGCTTCGT GAGTGGCATC ACGGGCATTG TGACCAAACC CATTAAAGGA
    GCTCAGAAGG AAGGAGCGGC AGGTTTCTTC AAGGGTATGG GTAAGGGTTT AGTTGGAGCT
    CTGACCAGAC CAACAGGGGG CATCATTGAC ATGGCCAGCA GCACCTTCCA GGGCATCAAG
    AGAGCTGCAG AGCTGTCTCA GGATGTGGAG AGTCTGCGCC CTCCACGCTT CATCCACGAG
    GACGGAGTGA TCCGGCCGTA CAGAGAGAGA GAAGGCCTCG GCAGTCAGAT GCTGCAGAGT
    ATTGAAAACG GACGGTTTGC CAAGTACAGG TACTTTGCTC ACGCTAAAGT CAACGAGTCT
    GATTTCCTGA TGATCACTAA AAGGGGGATC TTTTTCGTGA CAAAGGGCAC GTTTGGTCAG
    TTGACATGTG AATGGCAGTA TCTGTTCGAT GAGTTTACTA AAGCACCCAT GATTGTGGAA
    GGCAGGAGAC TGCGCATCGA AGCGAAGGAG AGAGTCAAGT CAGTGTTTCA CGCCAAAGAG
    TTCGGGAAGA TCATCAATTT CAAAACTCCA GAATTAGCAA AGTGGGTTCT TGCCAAGCTT
    GAAGACGCTC GGGAGAATCT GCCCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa40321
    Clone ID Related Accession (Same CDS sequence) XM_009304309.3 , XM_009304309.2
    Accession Version XM_009304309.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product vacuolar protein sorting-associated protein 13A isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304309.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCTCTCAGGC CGAGATCGAT GAACTGAGTC CAGGTCAGGC GGTTTACTAC
    ACCTGGGCTG AACCAACCGG CTCCAGGATC CTCAGCTGGA AACTGGGCAA ATACAAAGGA
    GAGCTCAAGA GTGAAGAGGA TCAGTTGCTG GATGTGGACA AGGAGGGCAA GTTGTTTTTG
    GTGTCGTTTT ATGAAGGCCT CCAGCGTGTG GCGCTCTTCA CCAGCGAGCA GCGCATCTTC
    AAGAAGATCT GCGACAGTGA GAAGATAGAG CTGGCTCAGC AAGAAATCAG CATCTTGCTG
    CAGAACATGG GCATTTCGCT GGTCAACAAC AACTCCAGCC AGGAAGTGGC CTTCATTGGC
    ATCACCAGCT CTGATGTTGT GTGGGAAACC AAGCCGAAGA AGCGCAGCCG CTGGAAAGTT
    CTGAGTGTTA AAGAGGCAGC AGTTCTGGAG CAGCACTACA GAGACTACAT GGAGAACGGC
    CCAGTGGACA GCACCATCAT AGACCTGGAC AACGACTACC AGGTGTTGTT TGCTGCTAAC
    GGTGTGGACA TGAGGATCAT GCAGCCCTAC GATGCTCCAC TCAGAAGGAA CTTCCTGCCG
    GCGTTGAAGG TGGAGTACAG CGTTTCAGCC CGCCAGAAAA CATACAGAGT CCAGATCAAC
    AGGATCCAGA TCCAAAACCA GCTGCAAGGA GCCATATTCC CGTTTGTGTT TTACCCCATC
    AAACCGCCAA AGTCTGTCAG CATGGACTCA GAGCCAAAAC CGCTGGCTGA CGTGAGCATC
    ATCACCAGAG CAGCAGGACA CTCCGACATT CTGCGCATCA AATATTTTAT GGTGTTGATT
    CAGGAGATGG ACCTGCGGCT GGATCTGGGC TTTCTGTATG CCATACTTGA GCTCTTCACT
    GCTGAACACA CCAGTGCTGT GACGTCTGAA CAGGAGGTGG AGTTGTTTGA TAAAGACATA
    GAGTATCTAA AGACTGAGTT AAATCATGTT TCAGCTACAG ACACATCTCC CATCAGCCTC
    TACGAGTACT TCCACATCTC TCCTATAAAG CTTCACTTGA GCTTGTCTCT GAGCAGTGGT
    GGTGAAGACA GCAATAAGCA AGAGCGCGAG ACGGAGCTGA TCCCAGTTCA GTCTCTCCAT
    CTGCTGCTCA AGAGCATCGG GGCTACACTT ACAGACGCAC AGGACGTCCT CTTTAAACTG
    GGCTTCTTCG AGCTGAGGTA TCAGTTTCGC ACCACTCAGC AGCTGCAGTG GGAGGTCGTT
    CGCCATTACT CCAAACAGGC TATCAAGCAG ATGTATGTCC TTGTTCTGGG TTTGGACGTT
    CTTGGGAATC CATTCGGGCT GATCCGGGGC CTGTCTGAAG GCGTCGAGGC TTTCTTTTAC
    GAGCCCTATC AGGGTGCAAT TCAGGGTCCA GAGGAGTTCA TGGAGGGCAT GGCTCTCGGC
    GTGAAGGCTC TGGTTGGAGG CGCTGTGGGT GGTCTGGCCG GAGCGGCGTC TCGCATCACT
    GGAGCGATGG CTAAAGGTGT GGCGGCGATG ACGATGGATG AAGAGTACCA GCAGAAGAGA
    CGAGAGGCCA TGAACAAACA GCCCAGCGGA CTGAGAGAAG GACTGACACG AGGAGGAAAA
    GGACTGGTGT CTGGCTTCGT GAGTGGCATC ACGGGCATTG TGACCAAACC CATTAAAGGA
    GCTCAGAAGG AAGGAGCGGC AGGTTTCTTC AAGGGTATGG GTAAGGGTTT AGTTGGAGCT
    CTGACCAGAC CAACAGGGGG CATCATTGAC ATGGCCAGCA GCACCTTCCA GGGCATCAAG
    AGAGCTGCAG AGCTGTCTCA GGATGTGGAG AGTCTGCGCC CTCCACGCTT CATCCACGAG
    GACGGAGTGA TCCGGCCGTA CAGAGAGAGA GAAGGCCTCG GCAGTCAGAT GCTGCAGAGT
    ATTGAAAACG GACGGTTTGC CAAGTACAGG TACTTTGCTC ACGCTAAAGT CAACGAGTCT
    GATTTCCTGA TGATCACTAA AAGGGGGATC TTTTTCGTGA CAAAGGGCAC GTTTGGTCAG
    TTGACATGTG AATGGCAGTA TCTGTTCGAT GAGTTTACTA AAGCACCCAT GATTGTGGAA
    GGCAGGAGAC TGCGCATCGA AGCGAAGGAG AGAGTCAAGT CAGTGTTTCA CGCCAAAGAG
    TTCGGGAAGA TCATCAATTT CAAAACTCCA GAATTAGCAA AGTGGGTTCT TGCCAAGCTT
    GAAGACGCTC GGGAGAATCT GCCCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302584.1
    CDS152..9781
    Translation

    Target ORF information:

    RefSeq Version XM_009304309.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304309.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCTCTCAGGC CGAGATCGAT GAACTGAGTC CAGGTCAGGC GGTTTACTAC
    ACCTGGGCTG AACCAACCGG CTCCAGGATC CTCAGCTGGA AACTGGGCAA ATACAAAGGA
    GAGCTCAAGA GTGAAGAGGA TCAGTTGCTG GATGTGGACA AGGAGGGCAA GTTGTTTTTG
    GTGTCGTTTT ATGAAGGCCT CCAGCGTGTG GCGCTCTTCA CCAGCGAGCA GCGCATCTTC
    AAGAAGATCT GCGACAGTGA GAAGATAGAG CTGGCTCAGC AAGAAATCAG CATCTTGCTG
    CAGAACATGG GCATTTCGCT GGTCAACAAC AACTCCAGCC AGGAAGTGGC CTTCATTGGC
    ATCACCAGCT CTGATGTTGT GTGGGAAACC AAGCCGAAGA AGCGCAGCCG CTGGAAAGTT
    CTGAGTGTTA AAGAGGCAGC AGTTCTGGAG CAGCACTACA GAGACTACAT GGAGAACGGC
    CCAGTGGACA GCACCATCAT AGACCTGGAC AACGACTACC AGGTGTTGTT TGCTGCTAAC
    GGTGTGGACA TGAGGATCAT GCAGCCCTAC GATGCTCCAC TCAGAAGGAA CTTCCTGCCG
    GCGTTGAAGG TGGAGTACAG CGTTTCAGCC CGCCAGAAAA CATACAGAGT CCAGATCAAC
    AGGATCCAGA TCCAAAACCA GCTGCAAGGA GCCATATTCC CGTTTGTGTT TTACCCCATC
    AAACCGCCAA AGTCTGTCAG CATGGACTCA GAGCCAAAAC CGCTGGCTGA CGTGAGCATC
    ATCACCAGAG CAGCAGGACA CTCCGACATT CTGCGCATCA AATATTTTAT GGTGTTGATT
    CAGGAGATGG ACCTGCGGCT GGATCTGGGC TTTCTGTATG CCATACTTGA GCTCTTCACT
    GCTGAACACA CCAGTGCTGT GACGTCTGAA CAGGAGGTGG AGTTGTTTGA TAAAGACATA
    GAGTATCTAA AGACTGAGTT AAATCATGTT TCAGCTACAG ACACATCTCC CATCAGCCTC
    TACGAGTACT TCCACATCTC TCCTATAAAG CTTCACTTGA GCTTGTCTCT GAGCAGTGGT
    GGTGAAGACA GCAATAAGCA AGAGCGCGAG ACGGAGCTGA TCCCAGTTCA GTCTCTCCAT
    CTGCTGCTCA AGAGCATCGG GGCTACACTT ACAGACGCAC AGGACGTCCT CTTTAAACTG
    GGCTTCTTCG AGCTGAGGTA TCAGTTTCGC ACCACTCAGC AGCTGCAGTG GGAGGTCGTT
    CGCCATTACT CCAAACAGGC TATCAAGCAG ATGTATGTCC TTGTTCTGGG TTTGGACGTT
    CTTGGGAATC CATTCGGGCT GATCCGGGGC CTGTCTGAAG GCGTCGAGGC TTTCTTTTAC
    GAGCCCTATC AGGGTGCAAT TCAGGGTCCA GAGGAGTTCA TGGAGGGCAT GGCTCTCGGC
    GTGAAGGCTC TGGTTGGAGG CGCTGTGGGT GGTCTGGCCG GAGCGGCGTC TCGCATCACT
    GGAGCGATGG CTAAAGGTGT GGCGGCGATG ACGATGGATG AAGAGTACCA GCAGAAGAGA
    CGAGAGGCCA TGAACAAACA GCCCAGCGGA CTGAGAGAAG GACTGACACG AGGAGGAAAA
    GGACTGGTGT CTGGCTTCGT GAGTGGCATC ACGGGCATTG TGACCAAACC CATTAAAGGA
    GCTCAGAAGG AAGGAGCGGC AGGTTTCTTC AAGGGTATGG GTAAGGGTTT AGTTGGAGCT
    CTGACCAGAC CAACAGGGGG CATCATTGAC ATGGCCAGCA GCACCTTCCA GGGCATCAAG
    AGAGCTGCAG AGCTGTCTCA GGATGTGGAG AGTCTGCGCC CTCCACGCTT CATCCACGAG
    GACGGAGTGA TCCGGCCGTA CAGAGAGAGA GAAGGCCTCG GCAGTCAGAT GCTGCAGAGT
    ATTGAAAACG GACGGTTTGC CAAGTACAGG TACTTTGCTC ACGCTAAAGT CAACGAGTCT
    GATTTCCTGA TGATCACTAA AAGGGGGATC TTTTTCGTGA CAAAGGGCAC GTTTGGTCAG
    TTGACATGTG AATGGCAGTA TCTGTTCGAT GAGTTTACTA AAGCACCCAT GATTGTGGAA
    GGCAGGAGAC TGCGCATCGA AGCGAAGGAG AGAGTCAAGT CAGTGTTTCA CGCCAAAGAG
    TTCGGGAAGA TCATCAATTT CAAAACTCCA GAATTAGCAA AGTGGGTTCT TGCCAAGCTT
    GAAGACGCTC GGGAGAATCT GCCCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa40322
    Clone ID Related Accession (Same CDS sequence) XM_009304310.2 , XM_009304310.3
    Accession Version XM_009304310.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product vacuolar protein sorting-associated protein 13A isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    181
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT
    TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT
    CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG
    AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC
    AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC
    ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG
    CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC
    CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG
    AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC
    AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC
    CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA
    CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA
    ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC
    CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA
    CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC
    AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT
    GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG
    GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA
    GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG
    AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG
    ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA
    CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG
    CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC
    CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA
    GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC
    ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC
    GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT
    GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA
    GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT
    GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG
    GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC
    AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC
    CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC
    GGCAGTCAGA TGCTGCAGAG CAGAACTATC GTCTCTCAGT GGAAAACTAC TGATGAGGAC
    TCTGATGAGG ACCAGGAAAA TCAGCTTAAT GACTTCTATT TTCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302585.1
    CDS152..9619
    Translation

    Target ORF information:

    RefSeq Version XM_009304310.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304310.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT
    TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT
    CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG
    AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC
    AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC
    ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG
    CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC
    CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG
    AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC
    AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC
    CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA
    CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA
    ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC
    CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA
    CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC
    AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT
    GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG
    GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA
    GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG
    AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG
    ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA
    CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG
    CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC
    CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA
    GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC
    ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC
    GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT
    GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA
    GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT
    GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG
    GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC
    AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC
    CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC
    GGCAGTCAGA TGCTGCAGAG CAGAACTATC GTCTCTCAGT GGAAAACTAC TGATGAGGAC
    TCTGATGAGG ACCAGGAAAA TCAGCTTAAT GACTTCTATT TTCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa40322
    Clone ID Related Accession (Same CDS sequence) XM_009304310.2 , XM_009304310.3
    Accession Version XM_009304310.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product vacuolar protein sorting-associated protein 13A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304310.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT
    TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT
    CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG
    AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC
    AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC
    ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG
    CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC
    CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG
    AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC
    AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC
    CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA
    CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA
    ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC
    CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA
    CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC
    AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT
    GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG
    GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA
    GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG
    AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG
    ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA
    CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG
    CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC
    CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA
    GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC
    ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC
    GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT
    GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA
    GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT
    GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG
    GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC
    AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC
    CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC
    GGCAGTCAGA TGCTGCAGAG CAGAACTATC GTCTCTCAGT GGAAAACTAC TGATGAGGAC
    TCTGATGAGG ACCAGGAAAA TCAGCTTAAT GACTTCTATT TTCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302585.1
    CDS51..9518
    Translation

    Target ORF information:

    RefSeq Version XM_009304310.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304310.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
    AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC
    CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA
    CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG
    GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA
    GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG
    ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG
    GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC
    CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA
    AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC
    AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA
    GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC
    ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC
    AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG
    GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG
    CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT
    GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG
    GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG
    CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG
    AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG
    GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG
    GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA
    GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG
    CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC
    AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG
    TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG
    GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG
    TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT
    GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC
    GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA
    ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA
    CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC
    ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT
    GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG
    GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC
    AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT
    CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG
    TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG
    GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG
    CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT
    GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA
    GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC
    ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG
    CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT
    ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC
    AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG
    TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT
    TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT
    CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG
    GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG
    GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC
    TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT
    CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG
    AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT
    TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG
    ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG
    GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT
    AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC
    GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG
    ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT
    ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG
    AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC
    GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG
    GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC
    ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC
    AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC
    ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC
    CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG
    GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC
    AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC
    GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA
    CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG
    TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC
    ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT
    CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT
    CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT
    CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT
    GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC
    AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA
    ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT
    GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC
    AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG
    CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG
    AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC
    AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC
    GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC
    CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC
    ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG
    CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG
    GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG
    AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG
    GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC
    AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC
    TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT
    AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT
    CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA
    GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG
    AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC
    AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG
    ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC
    ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC
    TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG
    TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT
    GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC
    ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT
    GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC
    CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG
    CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC
    TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC
    GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC
    AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG
    ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT
    GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC
    AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC
    TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG
    CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC
    AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT
    TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC
    TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC
    TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA
    CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG
    GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT
    TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG
    TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT
    TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT
    CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG
    AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC
    AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC
    ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG
    CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC
    CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG
    AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC
    AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC
    CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA
    CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA
    ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC
    CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA
    CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC
    AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT
    GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG
    GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA
    GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG
    AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG
    ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA
    CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG
    CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC
    CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA
    GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC
    ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC
    GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT
    GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA
    GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT
    GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG
    GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC
    AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC
    CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC
    GGCAGTCAGA TGCTGCAGAG CAGAACTATC GTCTCTCAGT GGAAAACTAC TGATGAGGAC
    TCTGATGAGG ACCAGGAAAA TCAGCTTAAT GACTTCTATT TTCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.