Mlycd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mlycd
Entrez Gene ID	85239
Full Name	malonyl-CoA decarboxylase
Synonyms	Mcd
General protein information	Preferred Names malonyl-CoA decarboxylase Names malonyl-CoA decarboxylase, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12
Summary	plays a role in malonyl-CoA catabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053477.1	NP_445929.1	malonyl-CoA decarboxylase, mitochondrial

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mlycd	NP_064350.2
Arabidopsis thaliana (thale cress)	AT4G04320	NP_974511.1
Oryza sativa (rice)	Os09g0394100	NP_001063091.1
Xenopus tropicalis (tropical clawed frog)	mlycd	NP_001120413.1
Danio rerio (zebrafish)	LOC100334385	XP_002665369.2
Caenorhabditis elegans (roundworm)	mlcd-1	NP_001022561.1
Homo sapiens (human)	MLYCD	NP_036345.2
Gallus gallus (chicken)	MLYCD	XP_414174.4
Bos taurus (cattle)	MLYCD	NP_001092416.1
Rattus norvegicus (Norway rat)	Mlycd	NP_445929.1
Danio rerio (zebrafish)	mlycd	NP_001082972.1
Pan troglodytes (chimpanzee)	MLYCD	XP_523518.2
	MLYCD	XP_001112117.1
Canis lupus familiaris (dog)	MLYCD	XP_852152.2

Gene Ontology
Bibliography

Related articles in PubMed

Nutritional stress exacerbates hepatic steatosis induced by deletion of the histidine nucleotide-binding (Hint2) mitochondrial protein.
Martin J, Balmer ML, Rajendran S, Maurhofer O, Dufour JF, St-Pierre MV
American journal of physiology. Gastrointestinal and liver physiology310(7)G497-509(2016 Apr)

Crystal structures of malonyl-coenzyme A decarboxylase provide insights into its catalytic mechanism and disease-causing mutations.
Froese DS, Forouhar F, Tran TH, Vollmar M, Kim YS, Lew S, Neely H, Seetharaman J, Shen Y, Xiao R, Acton TB, Everett JK, Cannone G, Puranik S, Savitsky P, Krojer T, Pilka ES, Kiyani W, Lee WH, Marsden BD, von Delft F, Allerston CK, Spagnolo L, Gileadi O, Montelione GT, Oppermann U, Yue WW, Tong L
Structure (London, England : 1993)21(7)1182-92(2013 Jul)

SIRT4 coordinates the balance between lipid synthesis and catabolism by repressing malonyl CoA decarboxylase.
Laurent G, German NJ, Saha AK, de Boer VC, Davies M, Koves TR, Dephoure N, Fischer F, Boanca G, Vaitheesvaran B, Lovitch SB, Sharpe AH, Kurland IJ, Steegborn C, Gygi SP, Muoio DM, Ruderman NB, Haigis MC
Molecular cell50(5)686-98(2013 Jun)

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Ghosh D, Berg JM
Journal of the American Chemical Society132(11)3973-9(2010 Mar)

Malonyl CoenzymeA decarboxylase regulates lipid and glucose metabolism in human skeletal muscle.
Bouzakri K, Austin R, Rune A, Lassman ME, Garcia-Roves PM, Berger JP, Krook A, Chibalin AV, Zhang BB, Zierath JR
Diabetes57(6)1508-16(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

substantial amounts of MCD protein and activity were found in the cytosol, mitochondria and peroxisomes, the latter with the highest specific activity
Title: Malonyl-CoA decarboxylase is present in the cytosolic, mitochondrial and peroxisomal compartments of rat hepatocytes.

differential regulation of malonyl CoA decarboxylase expression and activity in the mitochondria by AMP kinase may potentially regulate malonyl-CoA levels at sites nearby CPT-I on the mitochondria
Title: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5'AMP-activated protein kinase (AMPK). Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique.

Increasing myocardial malonyl CoA levels decreases fatty acid oxidation & accelerates glucose oxidation in ex vivo rat hearts This switch in energy substrate preference improves cardiac function during & after ischemia.
Title: Malonyl coenzyme a decarboxylase inhibition protects the ischemic heart by inhibiting fatty acid oxidation and stimulating glucose oxidation.

Peroxisomal-proliferator-activated receptor alpha activates transcription of the hepatic malonyl-CoA decarboxylase gene.
Title: Peroxisomal-proliferator-activated receptor alpha activates transcription of the rat hepatic malonyl-CoA decarboxylase gene: a key regulation of malonyl-CoA level.

Genomic organization and characterization of the promoter of teh malonyl-CoA decarboxylase gene
Title: Genomic organization and characterization of the promoter of rat malonyl-CoA decarboxylase gene.

The following Mlycd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mlycd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12440	NM_053477.1 Latest version!	Rattus norvegicus malonyl-CoA decarboxylase (Mlycd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mlycd ( NM_053477.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445929.1 Rattus norvegicus malonyl-CoA decarboxylase (Mlycd), mRNA.

Price & Availability↑

CloneID	ORa12440
Clone ID Related Accession (Same CDS sequence)	NM_053477.1
Accession Version	NM_053477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	malonyl-CoA decarboxylase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ007704.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ007704.1, EV768436.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAGAGGCT TGGGGCCAAG CTTGAGGGCT CGGCGCCTGC TCCCACTGCG GTACCCGCCG 
CGGCCTCCGG GGCCTCGGGG ACCTAGGCTG TGCAGCGGGC TCACGGCTAG CGCCATGGAC 
GAGCTGCTAC GGCGAGCCGT GCCACCCACG CCGGCCTACG AGCTGCGCGA GAAGACGCCG 
GCCCCGGCCG AGGGGCAGTG CGCGGACTTC GTGAGCTTTT ACGGCGGCCT GGCCGAGGCG 
GCCCAGCGCG CCGAGCTGCT CGGCCGCCTG GCTCAGGGCT TCGGCGTGGA TCACGGCCAG 
GTAGCGGAGC AGAGCGCCGG AGTGCTGCAG CTGCGCCAGC AGTCGCGCGA GGCGGCCGTG 
CTGCTGCAGG CAGAGGACCG GCTACGCTAT GCCCTCGTGC CGCGATACCG TGGCCTCTTC 
CACCACATCA GTAAGCTGGA CGGCGGCGTG CGCTTCCTGG TACAGCTGCG GGCCGATCTG 
CTGGAGGCGC AGGCCCTCAA GCTGGTGGAA GGGCCGCACG TCCGGGAAAT GAACGGAGTG 
CTAAAAAGCA TGCTGTCCGA GTGGTTCTCC TCTGGCTTCC TGAACCTGGA GCGGGTCACC 
TGGCACTCGC CCTGTGAGGT GCTTCAGAAG ATCAGCGATT GTGAGGCTGT GCAGCCTGTG 
AAAAACTGGA TGGACATGAA GCGGCGTGTG GGGCCATACC GGAGGTGTTA CTTCTTCTCC 
CACTGCTCCA CCCCCGGGGA CCCCCTGGTT GTTCTGCATG TGGCTCTGAC CGGTGACATT 
TCCAACAACA TCCAGAGCAT TGTGAAAGAG TGCCCTCCGT CTGAAACAGA GGAGAAGAAC 
CGGATCGCTG CTGCTGTCTT CTACTCCATC AGCCTGACCC AGCAGGGCCT GCAGGGCGTG 
GGACTCGGCA CCTTCCTCAT AAAGCGAGTG GTCAAGGAGC TGCAGAAGGA GTTTCCTCAT 
CTGGGGGCCT TTTCCAGCCT GTCACCTATA CCCGGATTCA CCAAGTGGCT GCTGGGCCTT 
CTGAATGTGC AGGGAAAGGA GTATGGGAGG AACGAGCTGT TCACAGACTC AGAGTGCAAA 
GAAATCGCAG AGGTCACGGG CGACCCTGTT CACGAGAGCC TCAAGGGTCT CCTGAGCAGT 
GGTGAGTGGG CGAAGTCTGA GAAGCTGGCA CAGGCACTGC AGGGGCCACT GATGAGACTG 
TGTGCCTGGT ACCTTTACGG TGAGAAGCAC CGATACGCCC TCAATCCAGT GGCCAACTTC 
CACCTGCAGA ACGGGGCTGT GATGTGGCGT ATCAACTGGA TGGCTGACAG CAGCCTCAAA 
GGCCTCACCA GCTCGTGCGG CCTCATGGTC AACTACCGTT ACTACCTGGA GGAGACCGGC 
CCCAACAGCA TCTCCTACCT GGGCTCCAAG AACATCAAAG CTTCCGAGCA GATCCTCAGC 
CTGGTAGCCC AGTTCCAGAG CAACAGCAAA CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445929.1
CDS	1..1476
Translation	MRGLGPSLRARRLLPLRYPPRPPGPRGPRLCSGLTASAMDELLRRAVPPTPAYELREKTPAPAEGQCADFVSFYGGLAEAAQRAELLGRLAQGFGVDHGQVAEQSAGVLQLRQQSREAAVLLQAEDRLRYALVPRYRGLFHHISKLDGGVRFLVQLRADLLEAQALKLVEGPHVREMNGVLKSMLSEWFSSGFLNLERVTWHSPCEVLQKISDCEAVQPVKNWMDMKRRVGPYRRCYFFSHCSTPGDPLVVLHVALTGDISNNIQSIVKECPPSETEEKNRIAAAVFYSISLTQQGLQGVGLGTFLIKRVVKELQKEFPHLGAFSSLSPIPGFTKWLLGLLNVQGKEYGRNELFTDSECKEIAEVTGDPVHESLKGLLSSGEWAKSEKLAQALQGPLMRLCAWYLYGEKHRYALNPVANFHLQNGAVMWRINWMADSSLKGLTSSCGLMVNYRYYLEETGPNSISYLGSKNIKASEQILSLVAQFQSNSKL

Target ORF information:

RefSeq Version	NM_053477.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus malonyl-CoA decarboxylase (Mlycd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053477.1

ORF Insert Sequence:

ATGAGAGGCT TGGGGCCAAG CTTGAGGGCT CGGCGCCTGC TCCCACTGCG GTACCCGCCG 
CGGCCTCCGG GGCCTCGGGG ACCTAGGCTG TGCAGCGGGC TCACGGCTAG CGCCATGGAC 
GAGCTGCTAC GGCGAGCCGT GCCACCCACG CCGGCCTACG AGCTGCGCGA GAAGACGCCG 
GCCCCGGCCG AGGGGCAGTG CGCGGACTTC GTGAGCTTTT ACGGCGGCCT GGCCGAGGCG 
GCCCAGCGCG CCGAGCTGCT CGGCCGCCTG GCTCAGGGCT TCGGCGTGGA TCACGGCCAG 
GTAGCGGAGC AGAGCGCCGG AGTGCTGCAG CTGCGCCAGC AGTCGCGCGA GGCGGCCGTG 
CTGCTGCAGG CAGAGGACCG GCTACGCTAT GCCCTCGTGC CGCGATACCG TGGCCTCTTC 
CACCACATCA GTAAGCTGGA CGGCGGCGTG CGCTTCCTGG TACAGCTGCG GGCCGATCTG 
CTGGAGGCGC AGGCCCTCAA GCTGGTGGAA GGGCCGCACG TCCGGGAAAT GAACGGAGTG 
CTAAAAAGCA TGCTGTCCGA GTGGTTCTCC TCTGGCTTCC TGAACCTGGA GCGGGTCACC 
TGGCACTCGC CCTGTGAGGT GCTTCAGAAG ATCAGCGATT GTGAGGCTGT GCAGCCTGTG 
AAAAACTGGA TGGACATGAA GCGGCGTGTG GGGCCATACC GGAGGTGTTA CTTCTTCTCC 
CACTGCTCCA CCCCCGGGGA CCCCCTGGTT GTTCTGCATG TGGCTCTGAC CGGTGACATT 
TCCAACAACA TCCAGAGCAT TGTGAAAGAG TGCCCTCCGT CTGAAACAGA GGAGAAGAAC 
CGGATCGCTG CTGCTGTCTT CTACTCCATC AGCCTGACCC AGCAGGGCCT GCAGGGCGTG 
GGACTCGGCA CCTTCCTCAT AAAGCGAGTG GTCAAGGAGC TGCAGAAGGA GTTTCCTCAT 
CTGGGGGCCT TTTCCAGCCT GTCACCTATA CCCGGATTCA CCAAGTGGCT GCTGGGCCTT 
CTGAATGTGC AGGGAAAGGA GTATGGGAGG AACGAGCTGT TCACAGACTC AGAGTGCAAA 
GAAATCGCAG AGGTCACGGG CGACCCTGTT CACGAGAGCC TCAAGGGTCT CCTGAGCAGT 
GGTGAGTGGG CGAAGTCTGA GAAGCTGGCA CAGGCACTGC AGGGGCCACT GATGAGACTG 
TGTGCCTGGT ACCTTTACGG TGAGAAGCAC CGATACGCCC TCAATCCAGT GGCCAACTTC 
CACCTGCAGA ACGGGGCTGT GATGTGGCGT ATCAACTGGA TGGCTGACAG CAGCCTCAAA 
GGCCTCACCA GCTCGTGCGG CCTCATGGTC AACTACCGTT ACTACCTGGA GGAGACCGGC 
CCCAACAGCA TCTCCTACCT GGGCTCCAAG AACATCAAAG CTTCCGAGCA GATCCTCAGC 
CTGGTAGCCC AGTTCCAGAG CAACAGCAAA CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nutritional stress exacerbates hepatic steatosis induced by deletion of the histidine nucleotide-binding (Hint2) mitochondrial protein.
American journal of physiology. Gastrointestinal and liver physiology310(7)G497-509(2016 Apr)
Martin J,Balmer ML,Rajendran S,Maurhofer O,Dufour JF,St-Pierre MV

Crystal structures of malonyl-coenzyme A decarboxylase provide insights into its catalytic mechanism and disease-causing mutations.
Structure (London, England : 1993)21(7)1182-92(2013 Jul)
Froese DS,Forouhar F,Tran TH,Vollmar M,Kim YS,Lew S,Neely H,Seetharaman J,Shen Y,Xiao R,Acton TB,Everett JK,Cannone G,Puranik S,Savitsky P,Krojer T,Pilka ES,Kiyani W,Lee WH,Marsden BD,von Delft F,Allerston CK,Spagnolo L,Gileadi O,Montelione GT,Oppermann U,Yue WW,Tong L

SIRT4 coordinates the balance between lipid synthesis and catabolism by repressing malonyl CoA decarboxylase.
Molecular cell50(5)686-98(2013 Jun)
Laurent G,German NJ,Saha AK,de Boer VC,Davies M,Koves TR,Dephoure N,Fischer F,Boanca G,Vaitheesvaran B,Lovitch SB,Sharpe AH,Kurland IJ,Steegborn C,Gygi SP,Muoio DM,Ruderman NB,Haigis MC

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Journal of the American Chemical Society132(11)3973-9(2010 Mar)
Ghosh D,Berg JM

Malonyl CoenzymeA decarboxylase regulates lipid and glucose metabolism in human skeletal muscle.
Diabetes57(6)1508-16(2008 Jun)
Bouzakri K,Austin R,Rune A,Lassman ME,Garcia-Roves PM,Berger JP,Krook A,Chibalin AV,Zhang BB,Zierath JR

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Mlycd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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