Arf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arf1
Entrez Gene ID	64310
Full Name	ADP-ribosylation factor 1
General protein information	Preferred Names ADP-ribosylation factor 1 Names ADP-ribosylation factor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q22
Summary	plays a role in the activation of non-voltage-activated Na(+)-selective current [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008767870.2	XP_008766092.1	ADP-ribosylation factor 1 isoform X1
XM_006246525.3	XP_006246587.1	ADP-ribosylation factor 1 isoform X2
NM_022518.3	NP_071963.1	ADP-ribosylation factor 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARF1	XP_001145129.1
Bos taurus (cattle)	ARF1	NP_788826.1
Gallus gallus (chicken)	ARF1	NP_001006352.1
Pan troglodytes (chimpanzee)	LOC457177	XP_513698.2
Canis lupus familiaris (dog)	LOC607365	NP_001238856.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012014	XP_320516.2
Homo sapiens (human)	ARF1	NP_001019399.1
Mus musculus (house mouse)	Arf1	NP_031502.1
Rattus norvegicus (Norway rat)	Arf1	NP_071963.1
Xenopus tropicalis (tropical clawed frog)	arf5	NP_989018.1
	ARF1	XP_002801945.1
Caenorhabditis elegans (roundworm)	arf-1.2	NP_498235.1
Danio rerio (zebrafish)	arf1	NP_958860.1
Drosophila melanogaster (fruit fly)	Arf79F	NP_730760.1

Gene Ontology
Bibliography

Related articles in PubMed

Hepatitis C virus triggers Golgi fragmentation and autophagy through the immunity-related GTPase M.
Hansen MD, Johnsen IB, Stiberg KA, Sherstova T, Wakita T, Richard GM, Kandasamy RK, Meurs EF, Anthonsen MW
Proceedings of the National Academy of Sciences of the United States of America114(17)E3462-E3471(2017 Apr)

The Arf GAP Asap promotes Arf1 function at the Golgi for cleavage furrow biosynthesis in Drosophila.
Rodrigues FF, Shao W, Harris TJ
Molecular biology of the cell27(20)3143-3155(2016 Oct)

The small GTPase Arf1 modulates Arp2/3-mediated actin polymerization via PICK1 to regulate synaptic plasticity.
Rocca DL, Amici M, Antoniou A, Blanco Suarez E, Halemani N, Murk K, McGarvey J, Jaafari N, Mellor JR, Collingridge GL, Hanley JG
Neuron79(2)293-307(2013 Jul)

GBF1 bears a novel phosphatidylinositol-phosphate binding module, BP3K, to link PI3K? activity with Arf1 activation involved in GPCR-mediated neutrophil chemotaxis and superoxide production.
Mazaki Y, Nishimura Y, Sabe H
Molecular biology of the cell23(13)2457-67(2012 Jul)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Arf1 is a critical regulator of actin dynamics and synaptic function via modulation of PICK1.
Title: The small GTPase Arf1 modulates Arp2/3-mediated actin polymerization via PICK1 to regulate synaptic plasticity.

ARF1-dependent trafficking of procathepsin B and the maturation of autophagosomes results in cathepsin B-mediated trypsinogen activation induced by caerulein.
Title: ADP-ribosylation factor 1 protein regulates trypsinogen activation via organellar trafficking of procathepsin B protein and autophagic maturation in acute pancreatitis.

Egr1 mediates p53-independent c-Myc-induced apoptosis via a noncanonical ARF-dependent transcriptional mechanism
Title: Egr1 mediates p53-independent c-Myc-induced apoptosis via a noncanonical ARF-dependent transcriptional mechanism.

Arf1-GTP-induced tubule formation suggests a function of Arf family proteins in curvature acquisition at sites of vesicle budding.
Title: Arf1-GTP-induced tubule formation suggests a function of Arf family proteins in curvature acquisition at sites of vesicle budding.

p24A is a regulator of signal-dependent trafficking that controls ARF1-dependent resensitization of PAR-2
Title: p24A, a type I transmembrane protein, controls ARF1-dependent resensitization of protease-activated receptor-2 by influence on receptor trafficking.

The following Arf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05821	NM_022518.3 Latest version!	Rattus norvegicus ADP-ribosylation factor 1 (Arf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa05821	XM_006246525.3 Latest version!	Rattus norvegicus ADP-ribosylation factor 1 (Arf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa33197	XM_008767870.2 Latest version!	Rattus norvegicus ADP-ribosylation factor 1 (Arf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arf1 ( NM_022518.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071963.1 Rattus norvegicus ADP-ribosylation factor 1 (Arf1), mRNA.

Price & Availability↑

CloneID	ORa05821
Clone ID Related Accession (Same CDS sequence)	XM_006246525.3 , NM_022518.3
Accession Version	NM_022518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	ADP-ribosylation factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L12380.1. On Jun 10, 2007 this sequence version replaced NM_022518.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L12380.1, CK366603.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACAACAATTC TATACAAACT GAAGCTGGGC 
GAAATTGTGA CCACCATTCC CACCATTGGT TTCAACGTGG AGACTGTTGA ATACAAGAAT 
ATCAGCTTCA CCGTGTGGGA TGTGGGTGGC CAGGACAAGA TTCGGCCGCT GTGGCGCCAC 
TACTTCCAGA ACACCCAAGG CTTGATCTTC GTAGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAAGAGCT TATGAGGATG CTAGCTGAAG ATGAGCTCCG AGATGCCGTT 
CTCTTGGTGT TTGCCAACAA GCAGGACCTC CCCAATGCCA TGAATGCGGC CGAAATCACA 
GACAAGCTGG GGCTGCACTC TCTACGCCAC AGGAACTGGT ACATTCAGGC TACCTGTGCC 
ACCAGCGGAG ACGGGCTCTA TGAAGGACTA GATTGGCTGT CTAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071963.1
CDS	75..620
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	NM_022518.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP-ribosylation factor 1 (Arf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022518.3

ORF Insert Sequence:

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACAACAATTC TATACAAACT GAAGCTGGGC 
GAAATTGTGA CCACCATTCC CACCATTGGT TTCAACGTGG AGACTGTTGA ATACAAGAAT 
ATCAGCTTCA CCGTGTGGGA TGTGGGTGGC CAGGACAAGA TTCGGCCGCT GTGGCGCCAC 
TACTTCCAGA ACACCCAAGG CTTGATCTTC GTAGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAAGAGCT TATGAGGATG CTAGCTGAAG ATGAGCTCCG AGATGCCGTT 
CTCTTGGTGT TTGCCAACAA GCAGGACCTC CCCAATGCCA TGAATGCGGC CGAAATCACA 
GACAAGCTGG GGCTGCACTC TCTACGCCAC AGGAACTGGT ACATTCAGGC TACCTGTGCC 
ACCAGCGGAG ACGGGCTCTA TGAAGGACTA GATTGGCTGT CTAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arf1 ( XM_006246525.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246587.1 Rattus norvegicus ADP-ribosylation factor 1 (Arf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa05821
Clone ID Related Accession (Same CDS sequence)	XM_006246525.3 , NM_022518.3
Accession Version	XM_006246525.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ADP-ribosylation factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246525.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACAACAATTC TATACAAACT GAAGCTGGGC 
GAAATTGTGA CCACCATTCC CACCATTGGT TTCAACGTGG AGACTGTTGA ATACAAGAAT 
ATCAGCTTCA CCGTGTGGGA TGTGGGTGGC CAGGACAAGA TTCGGCCGCT GTGGCGCCAC 
TACTTCCAGA ACACCCAAGG CTTGATCTTC GTAGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAAGAGCT TATGAGGATG CTAGCTGAAG ATGAGCTCCG AGATGCCGTT 
CTCTTGGTGT TTGCCAACAA GCAGGACCTC CCCAATGCCA TGAATGCGGC CGAAATCACA 
GACAAGCTGG GGCTGCACTC TCTACGCCAC AGGAACTGGT ACATTCAGGC TACCTGTGCC 
ACCAGCGGAG ACGGGCTCTA TGAAGGACTA GATTGGCTGT CTAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246587.1
CDS	144..689
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	XM_006246525.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP-ribosylation factor 1 (Arf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246525.3

ORF Insert Sequence:

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACAACAATTC TATACAAACT GAAGCTGGGC 
GAAATTGTGA CCACCATTCC CACCATTGGT TTCAACGTGG AGACTGTTGA ATACAAGAAT 
ATCAGCTTCA CCGTGTGGGA TGTGGGTGGC CAGGACAAGA TTCGGCCGCT GTGGCGCCAC 
TACTTCCAGA ACACCCAAGG CTTGATCTTC GTAGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAAGAGCT TATGAGGATG CTAGCTGAAG ATGAGCTCCG AGATGCCGTT 
CTCTTGGTGT TTGCCAACAA GCAGGACCTC CCCAATGCCA TGAATGCGGC CGAAATCACA 
GACAAGCTGG GGCTGCACTC TCTACGCCAC AGGAACTGGT ACATTCAGGC TACCTGTGCC 
ACCAGCGGAG ACGGGCTCTA TGAAGGACTA GATTGGCTGT CTAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arf1 ( XM_008767870.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766092.1 Rattus norvegicus ADP-ribosylation factor 1 (Arf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33197
Clone ID Related Accession (Same CDS sequence)	XM_008767870.2
Accession Version	XM_008767870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ADP-ribosylation factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTCT CTGGCCAGTA TCGCTTCCAC CTGTCCACAA ACATGGGGAA TATCTTTGCA 
AACCTCTTCA AGGGCCTTTT TGGCAAAAAA GAAATGCGCA TTCTCATGGT GGGCCTGGAT 
GCTGCAGGGA AGACAACAAT TCTATACAAA CTGAAGCTGG GCGAAATTGT GACCACCATT 
CCCACCATTG GTTTCAACGT GGAGACTGTT GAATACAAGA ATATCAGCTT CACCGTGTGG 
GATGTGGGTG GCCAGGACAA GATTCGGCCG CTGTGGCGCC ACTACTTCCA GAACACCCAA 
GGCTTGATCT TCGTAGTGGA CAGCAATGAC AGAGAGCGTG TGAACGAGGC CCGTGAAGAG 
CTTATGAGGA TGCTAGCTGA AGATGAGCTC CGAGATGCCG TTCTCTTGGT GTTTGCCAAC 
AAGCAGGACC TCCCCAATGC CATGAATGCG GCCGAAATCA CAGACAAGCT GGGGCTGCAC 
TCTCTACGCC ACAGGAACTG GTACATTCAG GCTACCTGTG CCACCAGCGG AGACGGGCTC 
TATGAAGGAC TAGATTGGCT GTCTAATCAG CTCCGGAACC AGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766092.1
CDS	124..711
Translation	MWVSGQYRFHLSTNMGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	XM_008767870.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP-ribosylation factor 1 (Arf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767870.2

ORF Insert Sequence:

ATGTGGGTCT CTGGCCAGTA TCGCTTCCAC CTGTCCACAA ACATGGGGAA TATCTTTGCA 
AACCTCTTCA AGGGCCTTTT TGGCAAAAAA GAAATGCGCA TTCTCATGGT GGGCCTGGAT 
GCTGCAGGGA AGACAACAAT TCTATACAAA CTGAAGCTGG GCGAAATTGT GACCACCATT 
CCCACCATTG GTTTCAACGT GGAGACTGTT GAATACAAGA ATATCAGCTT CACCGTGTGG 
GATGTGGGTG GCCAGGACAA GATTCGGCCG CTGTGGCGCC ACTACTTCCA GAACACCCAA 
GGCTTGATCT TCGTAGTGGA CAGCAATGAC AGAGAGCGTG TGAACGAGGC CCGTGAAGAG 
CTTATGAGGA TGCTAGCTGA AGATGAGCTC CGAGATGCCG TTCTCTTGGT GTTTGCCAAC 
AAGCAGGACC TCCCCAATGC CATGAATGCG GCCGAAATCA CAGACAAGCT GGGGCTGCAC 
TCTCTACGCC ACAGGAACTG GTACATTCAG GCTACCTGTG CCACCAGCGG AGACGGGCTC 
TATGAAGGAC TAGATTGGCT GTCTAATCAG CTCCGGAACC AGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hepatitis C virus triggers Golgi fragmentation and autophagy through the immunity-related GTPase M.
Proceedings of the National Academy of Sciences of the United States of America114(17)E3462-E3471(2017 Apr)
Hansen MD,Johnsen IB,Stiberg KA,Sherstova T,Wakita T,Richard GM,Kandasamy RK,Meurs EF,Anthonsen MW

The Arf GAP Asap promotes Arf1 function at the Golgi for cleavage furrow biosynthesis in Drosophila.
Molecular biology of the cell27(20)3143-3155(2016 Oct)
Rodrigues FF,Shao W,Harris TJ

The small GTPase Arf1 modulates Arp2/3-mediated actin polymerization via PICK1 to regulate synaptic plasticity.
Neuron79(2)293-307(2013 Jul)
Rocca DL,Amici M,Antoniou A,Blanco Suarez E,Halemani N,Murk K,McGarvey J,Jaafari N,Mellor JR,Collingridge GL,Hanley JG

GBF1 bears a novel phosphatidylinositol-phosphate binding module, BP3K, to link PI3K? activity with Arf1 activation involved in GPCR-mediated neutrophil chemotaxis and superoxide production.
Molecular biology of the cell23(13)2457-67(2012 Jul)
Mazaki Y,Nishimura Y,Sabe H

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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Arf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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