ARF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARF1
Entrez Gene ID	375
Full Name	ADP ribosylation factor 1
General protein information	Preferred Names ADP ribosylation factor 1 Names ADP-ribosylation factor 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.13
Summary	ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	103180

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024226.1	NP_001019397.1	ADP-ribosylation factor 1
NM_001658.4	NP_001649.1	ADP-ribosylation factor 1
NM_001024228.2	NP_001019399.1	ADP-ribosylation factor 1
NM_001024227.1	NP_001019398.1	ADP-ribosylation factor 1
NM_001024228.1	NP_001019399.1	ADP-ribosylation factor 1
NM_001658.3	NP_001649.1	ADP-ribosylation factor 1
XM_017001233.1	XP_016856722.1	ADP-ribosylation factor 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARF1	XP_001145129.1
Bos taurus (cattle)	ARF1	NP_788826.1
Gallus gallus (chicken)	ARF1	NP_001006352.1
Pan troglodytes (chimpanzee)	LOC457177	XP_513698.2
Canis lupus familiaris (dog)	LOC607365	NP_001238856.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012014	XP_320516.2
Homo sapiens (human)	ARF1	NP_001019399.1
Mus musculus (house mouse)	Arf1	NP_031502.1
Rattus norvegicus (Norway rat)	Arf1	NP_071963.1
Xenopus tropicalis (tropical clawed frog)	arf5	NP_989018.1
	ARF1	XP_002801945.1
Caenorhabditis elegans (roundworm)	arf-1.2	NP_498235.1
Danio rerio (zebrafish)	arf1	NP_958860.1
Drosophila melanogaster (fruit fly)	Arf79F	NP_730760.1

Gene Ontology
Bibliography

Related articles in PubMed

ARF1 recruits RAC1 to leading edge in neutrophil chemotaxis.
Mazaki Y, Onodera Y, Higashi T, Horinouchi T, Oikawa T, Sabe H
Cell communication and signaling : CCS15(1)36(2017 Oct)

Combined targeting of Arf1 and Ras potentiates anticancer activity for prostate cancer therapeutics.
Lang L, Shay C, Zhao X, Teng Y
Journal of experimental & clinical cancer research : CR36(1)112(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Experiments using a mutant form of ARF1 affecting GTP hydrolysis suggest that ARF1[GTP] is functionally required for the tubules to form.
Title: A novel physiological role for ARF1 in the formation of bidirectional tubules from the Golgi.

ARF1 may reverse CAM-DR by regulating phosphorylation of p27 at T157 in MM. our data shed new light on the molecular mechanism of CAM-DR in MM, and targeting ARF1 may be a novel therapeutic approach for improving the effectiveness of chemotherapy in MM.
Title: ADP-ribosylation factor 1 (ARF1) takes part in cell proliferation and cell adhesion-mediated drug resistance (CAM-DR).

ARNO-ARF1 regulates formation of podosomes by inhibition of RhoA/myosin-II and promotion of actin core assembly.
Title: Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO.

We report here that 2-methylcoprophilinamide (M-COPA), a compound that induces disassembly of the Golgi apparatus by inactivating ADP-ribosylation factor 1 (Arf1), suppresses Stx-induced apoptosis. M-COPA inhibited transport of Stx from the plasma membrane to the Golgi apparatus and suppressed degradation of anti-apoptotic proteins and the activation of caspases
Title: M-COPA, a novel Golgi system disruptor, suppresses apoptosis induced by Shiga toxin.

changes in distinct lipid ratios may converge on ARF1 to increase SBP-1/SREBP-1 activity.
Title: Cholesterol-Independent SREBP-1 Maturation Is Linked to ARF1 Inactivation.

The following ARF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17243	NM_001024228.2 Latest version!	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17243	NM_001658.4 Latest version!	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17243	NM_001024227.1 Latest version!	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17243	NM_001024226.1 Latest version!	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu87365	XM_017001233.1 Latest version!	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu17243	NM_001024228.1	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17243	NM_001658.3	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARF1 ( NM_001024226.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001019397.1 Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu17243
Clone ID Related Accession (Same CDS sequence)	NM_001024226.1 , NM_001658.4 , NM_001024228.2 , NM_001024227.1 , NM_001024228.1 , NM_001658.3
Accession Version	NM_001024226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Homo sapiens(human)
Product	ADP-ribosylation factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000620.1, BM844480.1 and BF337061.1. Transcript Variant: This variant (3) has an alternate and shorter 5' UTR, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.138700.1, SRR1803614.130644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019397.1
CDS	229..774
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	NM_001024226.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024226.1

ORF Insert Sequence:

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARF1 ( NM_001658.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001649.1 Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu17243
Clone ID Related Accession (Same CDS sequence)	NM_001024226.1 , NM_001658.4 , NM_001024228.2 , NM_001024227.1 , NM_001024228.1 , NM_001658.3
Accession Version	NM_001658.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	ADP-ribosylation factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000620.1 and BF337061.1. On Nov 22, 2018 this sequence version replaced NM_001658.3. Transcript Variant: This variant (4) has an alternate and shorter 5' UTR, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.686679.1, SRR3476690.739226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000272102.10/ ENSP00000272102.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001649.1
CDS	96..641
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	NM_001658.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001658.4

ORF Insert Sequence:

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARF1 ( NM_001024228.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001019399.1 Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17243
Clone ID Related Accession (Same CDS sequence)	NM_001024226.1 , NM_001658.4 , NM_001024228.2 , NM_001024227.1 , NM_001024228.1 , NM_001658.3
Accession Version	NM_001024228.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	ADP-ribosylation factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136379.19, BQ949763.1, BM353046.1, AK000620.1 and BF337061.1. On May 31, 2019 this sequence version replaced NM_001024228.1. Transcript Variant: This variant (2) lacks a segment in the 5' UTR, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.141165.1, SRR1803613.221056.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019399.1
CDS	257..802
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	NM_001024228.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024228.2

ORF Insert Sequence:

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARF1 ( NM_001024227.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001019398.1 Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17243
Clone ID Related Accession (Same CDS sequence)	NM_001024226.1 , NM_001658.4 , NM_001024228.2 , NM_001024227.1 , NM_001024228.1 , NM_001658.3
Accession Version	NM_001024227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	ADP-ribosylation factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG421840.1, BQ935470.1, AK000620.1 and BF337061.1. Transcript Variant: This variant (1) represents the longest transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.27707.1, SRR1163655.159587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019398.1
CDS	263..808
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	NM_001024227.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024227.1

ORF Insert Sequence:

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARF1 ( NM_001024228.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001019399.1 Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17243
Clone ID Related Accession (Same CDS sequence)	NM_001024226.1 , NM_001658.4 , NM_001024228.2 , NM_001024227.1 , NM_001024228.1 , NM_001658.3
Accession Version	NM_001024228.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	ADP-ribosylation factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ949763.1, BM353046.1, AK000620.1 and BF337061.1. Transcript Variant: This variant (2) lacks a segment in the 5' UTR, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.141165.1, SRR1803613.221056.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019399.1
CDS	241..786
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	NM_001024228.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024228.1

ORF Insert Sequence:

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARF1 ( NM_001658.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001649.1 Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 4, mRNA.

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CloneID	OHu17243
Clone ID Related Accession (Same CDS sequence)	NM_001024226.1 , NM_001658.4 , NM_001024228.2 , NM_001024227.1 , NM_001024228.1 , NM_001658.3
Accession Version	NM_001658.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	ADP-ribosylation factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000620.1 and BF337061.1. On Jun 2, 2005 this sequence version replaced NM_001658.2. Transcript Variant: This variant (4) has an alternate and shorter 5' UTR, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000620.1, SRR1803614.220133.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001649.1
CDS	144..689
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	NM_001658.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001658.3

ORF Insert Sequence:

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGACCTC CCCAACGCCA TGAATGCGGC CGAGATCACA 
GACAAGCTGG GGCTGCACTC ACTACGCCAC AGGAACTGGT ACATTCAGGC CACCTGCGCC 
ACCAGCGGCG ACGGGCTCTA TGAAGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARF1 ( XM_017001233.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856722.1 Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu87365
Clone ID Related Accession (Same CDS sequence)	XM_017001233.1
Accession Version	XM_017001233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ADP-ribosylation factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGTAGGC GCCCGGGCCA GCCTGGGGAA TGTGAGGAGC 
CAGTGTGGGT TCCGCCTGGT GGTAGGGGTT ACTGGAGGCT GGTGGGGCCC CTTTCTCTGT 
CCTGTGGACA GCCCTTCCCA CCAACCCTTC CTTCCCCCAG GACCTCCCCA ACGCCATGAA 
TGCGGCCGAG ATCACAGACA AGCTGGGGCT GCACTCACTA CGCCACAGGA ACTGGTACAT 
TCAGGCCACC TGCGCCACCA GCGGCGACGG GCTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856722.1
CDS	96..734
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQVGARASLGNVRSQCGFRLVVGVTGGWWGPFLCPVDSPSHQPFLPPGPPQRHECGRDHRQAGAALTTPQELVHSGHLRHQRRRAL

Target ORF information:

RefSeq Version	XM_017001233.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001233.1

ORF Insert Sequence:

ATGGGGAACA TCTTCGCCAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATC 
CTCATGGTGG GCCTGGATGC TGCAGGGAAG ACCACGATCC TCTACAAGCT TAAGCTGGGT 
GAGATCGTGA CCACCATTCC CACCATAGGC TTCAACGTGG AAACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCCCT GTGGCGCCAC 
TACTTCCAGA ACACACAAGG CCTGATCTTC GTGGTGGACA GCAATGACAG AGAGCGTGTG 
AACGAGGCCC GTGAGGAGCT CATGAGGATG CTGGCCGAGG ACGAGCTCCG GGATGCTGTC 
CTCCTGGTGT TCGCCAACAA GCAGGTAGGC GCCCGGGCCA GCCTGGGGAA TGTGAGGAGC 
CAGTGTGGGT TCCGCCTGGT GGTAGGGGTT ACTGGAGGCT GGTGGGGCCC CTTTCTCTGT 
CCTGTGGACA GCCCTTCCCA CCAACCCTTC CTTCCCCCAG GACCTCCCCA ACGCCATGAA 
TGCGGCCGAG ATCACAGACA AGCTGGGGCT GCACTCACTA CGCCACAGGA ACTGGTACAT 
TCAGGCCACC TGCGCCACCA GCGGCGACGG GCTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ARF1 recruits RAC1 to leading edge in neutrophil chemotaxis.
Cell communication and signaling : CCS15(1)36(2017 Oct)
Mazaki Y,Onodera Y,Higashi T,Horinouchi T,Oikawa T,Sabe H

Combined targeting of Arf1 and Ras potentiates anticancer activity for prostate cancer therapeutics.
Journal of experimental & clinical cancer research : CR36(1)112(2017 Aug)
Lang L,Shay C,Zhao X,Teng Y

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ARF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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