Herc6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Herc6
Entrez Gene ID	362376
Full Name	HECT and RLD domain containing E3 ubiquitin protein ligase family member 6
General protein information	Preferred Names HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 Names probable E3 ubiquitin-protein ligase HERC6 hect domain and RLD 6 potential ubiquitin ligase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q24

mRNA and Protein(s)

mRNA	Protein	Name
XM_008775746.2	XP_008773968.1	probable E3 ubiquitin-protein ligase HERC6 isoform X2
XM_008762963.2	XP_008761185.1	probable E3 ubiquitin-protein ligase HERC6 isoform X1

Pathways from BioSystems

Homologies

	HERC6	XP_002804182.1
Bos taurus (cattle)	HERC6	NP_001179573.1
Homo sapiens (human)	HERC6	NP_060382.3
Canis lupus familiaris (dog)	HERC6	XP_851549.1
Pan troglodytes (chimpanzee)	HERC6	XP_001160851.1
Xenopus tropicalis (tropical clawed frog)	herc6	XP_002938670.2
Mus musculus (house mouse)	Herc6	NP_080268.1
Rattus norvegicus (Norway rat)	Herc6	XP_006236645.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Herc6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Herc6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa48209	XM_008775746.2 Latest version!	Rattus norvegicus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (Herc6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa48209	XM_008762963.2 Latest version!	Rattus norvegicus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (Herc6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Herc6 ( XM_008775746.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008773968.1 Rattus norvegicus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (Herc6), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa48209
Clone ID Related Accession (Same CDS sequence)	XM_008775746.2 , XM_008762963.2
Accession Version	XM_008775746.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000072.1) and transcript sequence (BC085921.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008775746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases assembly gap :: added 151 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTACTTCA CCTGGGCTCC AGGAAGGCGG CAGCCTCGGC GCCTGGAGGC AGGAACTGGT 
CTTAAGCTCT TGAAGGCGGC CAGCGGGGAA CGCCACTCGC TGCTACTGTT CAGCAACCAC 
AGGGTGTACT CCTGCGGGGA CAACAGCAGG GGCCAGCTGG GCCAGAAGAG TCCGCAGAGC 
ACCAAACGGC CAGAACCAAT TCAGGCACTA AGTACTGTAC ACATTGACCT GGTGAGCTGT 
GGGAAGGAGC ACTCAGTGGC TGTCTGCCAC CAAGGAAGGG TCTTCACATG GGGAGCCGGT 
TCTGAAGGGC AGCTGGGGAT TGGAGAATCT AAGGAAATAA GCTTCATGCC TACGAAAATA 
AACTCTCTGG CTGGCATAAA AATAATCCAA GTTTCCTGTG GACACTACCA CTCTTTGGCA 
TTATCAGAAG ATGGCCAAGT GTTTTCATGG GGAAGTAACA GACAAGGACA ACTGGGCCTG 
GGGAATAATT TATGTTCCCA AGCCAGCCCA CAGAAGGTGA AGTCCCTGGA GGGGATCCCA 
CTGGCTCAAG TAGCTGCAGG TGGCACACAC AGTTTTGCTC TGTCTCTCAT GGGGACTTCA 
TTTGGCTGGG GGAACAACAG ATCTGGGCAG CTGGCCCTCA GTGGGAACAG TGCTAAAGAG 
CAAATCTACA AGCCTCATTC AATTGGTGCC CTGAAGACTC TCAATGTGGT TTATATTAGC 
TGTGGTTATG AGCACACTTC AGTGCTTACC GAGGATGGAC AAGTGTTTAC ATTTGGAGGC 
AGTAGCTCTG AACAGCTACA GCACAGCCCC AGGAGTGGGA GAGGAGGTCC ACAACTCATA 
GAAGGGATTG GCGGCCATGT TTCACAGATA GAATGTGGAA GTTATCACAC CATTGCATAT 
GTCTACACCA CTGGTCAGGT GGTATCTTTA GGCCGTGGAC CAAGTTGCAC AAGCAACGCA 
ACTCATCAGG AGGCTGCTTG CTCTGACGTT ACCTGCTTGC TATCTGCCAA GGACCTTGTG 
GATATTGAAG TCAAAGACAT TTTTGCTGGA GCACACGCCA ACTTTGTGAC AACCTGCCGG 
CATACTAGGT CCACAAGTGT TTCCATGAAA ATCCTGCCAG AAATTATCCG AATCACCCAG 
CCACTGGTAA AGAAATGGCG AGCAGCAAAG AAAGGAAAAG TCCATGAAGA CGCCAAAAGA 
GAAATCAGCT TGATATTTTC ATCTCCTGCT TGTTTGACTG CAAGTTTTTT GAAGAAAAGA 
GATGCTGGAG AGAATAATTT CATTAACGTG GACTTAAAAC TGGCAAGGGA TGTCTTCAAG 
ATGTTAACAG CAGAGAAATG GATTTCCTCC TTGATAACCA CTTGTCTTGA GGAGACTCTG 
CTTAGAGGTC TTCCATACAG CTCTCCACAT CAAGAAGCTC TATTAGTTTT TCTTTTGCTC 
CCGGAATGTT CCATTATGCA GGATCCCAAG AACTGGAAGA CACTGGCATT TGAATTTGCA 
AGCGCTATTC ATAAAATGAG GTCAAAGTCA TTAACATTCC TGAGAAACTG GTGGGCATCT 
TTGGAAGTTT CTTCTCTGAA CTCACTGGTC CAGATGCTAA AGAAAACCAT CGTCTCTCAA 
CTGCATAATG AAGTTCCAAT GATGAAGGAT ACGTCTAACC TTAAAGTTTC TTTAGAAATG 
ATGAAAGAAG TGCAAAAGAT ACTAGAGAAA ACCAACTCTC ATCTTCATAA TGAAGTTCCA 
AAGATGGAGG ATACGTCTAA TCTTAAAGTT CTCTTAGAAG TGATGAAAGA AGTGCATGAG 
GCTAACTGTC AACTTCCAGA AAATGCTTTC ATCATGAATG ACATCTTTAA CTTCAATACA 
GAAGAAGGAA TAATGGCCAA ACAGCAAATG TTCTTTATAA AATACTATTC TATGGTTTTC 
AATGATTTTC CATTTATCCT TGATTTGCCA TCCAAAATTA TATTAATGAA ACATGACTCA 
TCTGTAAAGC TGTCGGATGA AGTCAAAGTC GCTGCATCTC CAGAAAAGAT GGGATGCCCG 
TTCTTTATAC TGAAAGTCAG ACGAAGTCAT CTGGTGGAAG ACACCTTGCG TCAATTAAGA 
CAAGCTGAAG ATTTTGACCT TAGGAAGACA CTATCGGTCG GATTTATTAA TGAAATTCGT 
CCTGAGGGTG GGGGAGTAAG TTCTGAGTTC TTCCACTGTA TATTTGAAGA GATGACAGAC 
CCCAAATATG AAATGTTCAT GTATCCTGAA AATGGTTCCA ACATGTGGTT TCCAGTCAAT 
CCCAAGTTTG AGAAGAGCAG ATATTTCCTC TTTGGGATCT TATGTGGACT CTCCCTAAAT 
AATTTAAATG TTATCAACCT CTCTTTCCCA CTGGCTTTAT ATAAAAAACT TTTGGAGCAA 
AAGCCATCTT TGGAAGATTT AAAAGACCTT AGTCTTCTCC TGGGGAGGAA TTTGCAAGAA 
GTTCTAAATT GTGAGGCTGG TGTTATTGAA GAACTTCACA TGTATTTTTC TATATACTGG 
GATCAAAGAG ATGTTGATTT AATTCCAGAT GGGATCTCTG TGCCTGTGAA TGAAACAAAC 
AAGAGAGACT ATGTGTCCAA GTGTGTTGAT TACATTTTTA ACATTTCTAT AAAGACAATT 
TATGACGAAT TTCATAGAGG ATTTTACAAG GTGTGTAACC GGGACAGTAT TAGGCATTTC 
CAGCCTGAAG AACTAATGGC AGCAATAATT GGAAACCCTA CTTGTGACTG GAAACAATTT 
GAAAATAATT CAAAGTATGA AAATGGATAT TCCAAATCAC ACCCAACTAT TCTACTGTTT 
TGGAAGGCTT TCCATGAACT AACTTTGGAT GAAAAGAAAA AGTTCCTCTT GTTTCTTACA 
GGATGTGATA GGCTACATGT AAAAGGCCTA CAGAATGAAG GAATAAGATT CCGGTGTCCT 
GAAGTTTTCA GTGAAAGAGA TAACCCAAGA TCATTGACAT GTCACAGTAT TCTAGACCTG 
CCTAAGTATT CCACAATGAG AAGGATGAAG GAAGCACTTC AGGTTGCAAT CAACAATAAC 
AAGGGGTTTA TCTCACCGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008773968.1
CDS	245..3328
Translation	MYFTWAPGRRQPRRLEAGTGLKLLKAASGERHSLLLFSNHRVYSCGDNSRGQLGQKSPQSTKRPEPIQALSTVHIDLVSCGKEHSVAVCHQGRVFTWGAGSEGQLGIGESKEISFMPTKINSLAGIKIIQVSCGHYHSLALSEDGQVFSWGSNRQGQLGLGNNLCSQASPQKVKSLEGIPLAQVAAGGTHSFALSLMGTSFGWGNNRSGQLALSGNSAKEQIYKPHSIGALKTLNVVYISCGYEHTSVLTEDGQVFTFGGSSSEQLQHSPRSGRGGPQLIEGIGGHVSQIECGSYHTIAYVYTTGQVVSLGRGPSCTSNATHQEAACSDVTCLLSAKDLVDIEVKDIFAGAHANFVTTCRHTRSTSVSMKILPEIIRITQPLVKKWRAAKKGKVHEDAKREISLIFSSPACLTASFLKKRDAGENNFINVDLKLARDVFKMLTAEKWISSLITTCLEETLLRGLPYSSPHQEALLVFLLLPECSIMQDPKNWKTLAFEFASAIHKMRSKSLTFLRNWWASLEVSSLNSLVQMLKKTIVSQLHNEVPMMKDTSNLKVSLEMMKEVQKILEKTNSHLHNEVPKMEDTSNLKVLLEVMKEVHEANCQLPENAFIMNDIFNFNTEEGIMAKQQMFFIKYYSMVFNDFPFILDLPSKIILMKHDSSVKLSDEVKVAASPEKMGCPFFILKVRRSHLVEDTLRQLRQAEDFDLRKTLSVGFINEIRPEGGGVSSEFFHCIFEEMTDPKYEMFMYPENGSNMWFPVNPKFEKSRYFLFGILCGLSLNNLNVINLSFPLALYKKLLEQKPSLEDLKDLSLLLGRNLQEVLNCEAGVIEELHMYFSIYWDQRDVDLIPDGISVPVNETNKRDYVSKCVDYIFNISIKTIYDEFHRGFYKVCNRDSIRHFQPEELMAAIIGNPTCDWKQFENNSKYENGYSKSHPTILLFWKAFHELTLDEKKKFLLFLTGCDRLHVKGLQNEGIRFRCPEVFSERDNPRSLTCHSILDLPKYSTMRRMKEALQVAINNNKGFISPE

Target ORF information:

RefSeq Version	XM_008775746.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (Herc6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008775746.2

ORF Insert Sequence:

ATGTACTTCA CCTGGGCTCC AGGAAGGCGG CAGCCTCGGC GCCTGGAGGC AGGAACTGGT 
CTTAAGCTCT TGAAGGCGGC CAGCGGGGAA CGCCACTCGC TGCTACTGTT CAGCAACCAC 
AGGGTGTACT CCTGCGGGGA CAACAGCAGG GGCCAGCTGG GCCAGAAGAG TCCGCAGAGC 
ACCAAACGGC CAGAACCAAT TCAGGCACTA AGTACTGTAC ACATTGACCT GGTGAGCTGT 
GGGAAGGAGC ACTCAGTGGC TGTCTGCCAC CAAGGAAGGG TCTTCACATG GGGAGCCGGT 
TCTGAAGGGC AGCTGGGGAT TGGAGAATCT AAGGAAATAA GCTTCATGCC TACGAAAATA 
AACTCTCTGG CTGGCATAAA AATAATCCAA GTTTCCTGTG GACACTACCA CTCTTTGGCA 
TTATCAGAAG ATGGCCAAGT GTTTTCATGG GGAAGTAACA GACAAGGACA ACTGGGCCTG 
GGGAATAATT TATGTTCCCA AGCCAGCCCA CAGAAGGTGA AGTCCCTGGA GGGGATCCCA 
CTGGCTCAAG TAGCTGCAGG TGGCACACAC AGTTTTGCTC TGTCTCTCAT GGGGACTTCA 
TTTGGCTGGG GGAACAACAG ATCTGGGCAG CTGGCCCTCA GTGGGAACAG TGCTAAAGAG 
CAAATCTACA AGCCTCATTC AATTGGTGCC CTGAAGACTC TCAATGTGGT TTATATTAGC 
TGTGGTTATG AGCACACTTC AGTGCTTACC GAGGATGGAC AAGTGTTTAC ATTTGGAGGC 
AGTAGCTCTG AACAGCTACA GCACAGCCCC AGGAGTGGGA GAGGAGGTCC ACAACTCATA 
GAAGGGATTG GCGGCCATGT TTCACAGATA GAATGTGGAA GTTATCACAC CATTGCATAT 
GTCTACACCA CTGGTCAGGT GGTATCTTTA GGCCGTGGAC CAAGTTGCAC AAGCAACGCA 
ACTCATCAGG AGGCTGCTTG CTCTGACGTT ACCTGCTTGC TATCTGCCAA GGACCTTGTG 
GATATTGAAG TCAAAGACAT TTTTGCTGGA GCACACGCCA ACTTTGTGAC AACCTGCCGG 
CATACTAGGT CCACAAGTGT TTCCATGAAA ATCCTGCCAG AAATTATCCG AATCACCCAG 
CCACTGGTAA AGAAATGGCG AGCAGCAAAG AAAGGAAAAG TCCATGAAGA CGCCAAAAGA 
GAAATCAGCT TGATATTTTC ATCTCCTGCT TGTTTGACTG CAAGTTTTTT GAAGAAAAGA 
GATGCTGGAG AGAATAATTT CATTAACGTG GACTTAAAAC TGGCAAGGGA TGTCTTCAAG 
ATGTTAACAG CAGAGAAATG GATTTCCTCC TTGATAACCA CTTGTCTTGA GGAGACTCTG 
CTTAGAGGTC TTCCATACAG CTCTCCACAT CAAGAAGCTC TATTAGTTTT TCTTTTGCTC 
CCGGAATGTT CCATTATGCA GGATCCCAAG AACTGGAAGA CACTGGCATT TGAATTTGCA 
AGCGCTATTC ATAAAATGAG GTCAAAGTCA TTAACATTCC TGAGAAACTG GTGGGCATCT 
TTGGAAGTTT CTTCTCTGAA CTCACTGGTC CAGATGCTAA AGAAAACCAT CGTCTCTCAA 
CTGCATAATG AAGTTCCAAT GATGAAGGAT ACGTCTAACC TTAAAGTTTC TTTAGAAATG 
ATGAAAGAAG TGCAAAAGAT ACTAGAGAAA ACCAACTCTC ATCTTCATAA TGAAGTTCCA 
AAGATGGAGG ATACGTCTAA TCTTAAAGTT CTCTTAGAAG TGATGAAAGA AGTGCATGAG 
GCTAACTGTC AACTTCCAGA AAATGCTTTC ATCATGAATG ACATCTTTAA CTTCAATACA 
GAAGAAGGAA TAATGGCCAA ACAGCAAATG TTCTTTATAA AATACTATTC TATGGTTTTC 
AATGATTTTC CATTTATCCT TGATTTGCCA TCCAAAATTA TATTAATGAA ACATGACTCA 
TCTGTAAAGC TGTCGGATGA AGTCAAAGTC GCTGCATCTC CAGAAAAGAT GGGATGCCCG 
TTCTTTATAC TGAAAGTCAG ACGAAGTCAT CTGGTGGAAG ACACCTTGCG TCAATTAAGA 
CAAGCTGAAG ATTTTGACCT TAGGAAGACA CTATCGGTCG GATTTATTAA TGAAATTCGT 
CCTGAGGGTG GGGGAGTAAG TTCTGAGTTC TTCCACTGTA TATTTGAAGA GATGACAGAC 
CCCAAATATG AAATGTTCAT GTATCCTGAA AATGGTTCCA ACATGTGGTT TCCAGTCAAT 
CCCAAGTTTG AGAAGAGCAG ATATTTCCTC TTTGGGATCT TATGTGGACT CTCCCTAAAT 
AATTTAAATG TTATCAACCT CTCTTTCCCA CTGGCTTTAT ATAAAAAACT TTTGGAGCAA 
AAGCCATCTT TGGAAGATTT AAAAGACCTT AGTCTTCTCC TGGGGAGGAA TTTGCAAGAA 
GTTCTAAATT GTGAGGCTGG TGTTATTGAA GAACTTCACA TGTATTTTTC TATATACTGG 
GATCAAAGAG ATGTTGATTT AATTCCAGAT GGGATCTCTG TGCCTGTGAA TGAAACAAAC 
AAGAGAGACT ATGTGTCCAA GTGTGTTGAT TACATTTTTA ACATTTCTAT AAAGACAATT 
TATGACGAAT TTCATAGAGG ATTTTACAAG GTGTGTAACC GGGACAGTAT TAGGCATTTC 
CAGCCTGAAG AACTAATGGC AGCAATAATT GGAAACCCTA CTTGTGACTG GAAACAATTT 
GAAAATAATT CAAAGTATGA AAATGGATAT TCCAAATCAC ACCCAACTAT TCTACTGTTT 
TGGAAGGCTT TCCATGAACT AACTTTGGAT GAAAAGAAAA AGTTCCTCTT GTTTCTTACA 
GGATGTGATA GGCTACATGT AAAAGGCCTA CAGAATGAAG GAATAAGATT CCGGTGTCCT 
GAAGTTTTCA GTGAAAGAGA TAACCCAAGA TCATTGACAT GTCACAGTAT TCTAGACCTG 
CCTAAGTATT CCACAATGAG AAGGATGAAG GAAGCACTTC AGGTTGCAAT CAACAATAAC 
AAGGGGTTTA TCTCACCGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Herc6 ( XM_008762963.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761185.1 Rattus norvegicus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (Herc6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa48209
Clone ID Related Accession (Same CDS sequence)	XM_008775746.2 , XM_008762963.2
Accession Version	XM_008762963.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762963.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCA CCTGGGCTCC AGGAAGGCGG CAGCCTCGGC GCCTGGAGGC AGGAACTGGT 
CTTAAGCTCT TGAAGGCGGC CAGCGGGGAA CGCCACTCGC TGCTACTGTT CAGCAACCAC 
AGGGTGTACT CCTGCGGGGA CAACAGCAGG GGCCAGCTGG GCCAGAAGAG TCCGCAGAGC 
ACCAAACGGC CAGAACCAAT TCAGGCACTA AGTACTGTAC ACATTGACCT GGTGAGCTGT 
GGGAAGGAGC ACTCAGTGGC TGTCTGCCAC CAAGGAAGGG TCTTCACATG GGGAGCCGGT 
TCTGAAGGGC AGCTGGGGAT TGGAGAATCT AAGGAAATAA GCTTCATGCC TACGAAAATA 
AACTCTCTGG CTGGCATAAA AATAATCCAA GTTTCCTGTG GACACTACCA CTCTTTGGCA 
TTATCAGAAG ATGGCCAAGT GTTTTCATGG GGAAGTAACA GACAAGGACA ACTGGGCCTG 
GGGAATAATT TATGTTCCCA AGCCAGCCCA CAGAAGGTGA AGTCCCTGGA GGGGATCCCA 
CTGGCTCAAG TAGCTGCAGG TGGCACACAC AGTTTTGCTC TGTCTCTCAT GGGGACTTCA 
TTTGGCTGGG GGAACAACAG ATCTGGGCAG CTGGCCCTCA GTGGGAACAG TGCTAAAGAG 
CAAATCTACA AGCCTCATTC AATTGGTGCC CTGAAGACTC TCAATGTGGT TTATATTAGC 
TGTGGTTATG AGCACACTTC AGTGCTTACC GAGGATGGAC AAGTGTTTAC ATTTGGAGGC 
AGTAGCTCTG AACAGCTACA GCACAGCCCC AGGAGTGGGA GAGGAGGTCC ACAACTCATA 
GAAGGGATTG GCGGCCATGT TTCACAGATA GAATGTGGAA GTTATCACAC CATTGCATAT 
GTCTACACCA CTGGTCAGGT GGTATCTTTA GGCCGTGGAC CAAGTTGCAC AAGCAACGCA 
ACTCATCAGG AGGCTGCTTG CTCTGACGTT ACCTGCTTGC TATCTGCCAA GGACCTTGTG 
GATATTGAAG TCAAAGACAT TTTTGCTGGA GCACACGCCA ACTTTGTGAC AACCTGCCGG 
CATACTAGGT CCACAAGTGT TTCCATGAAA ATCCTGCCAG AAATTATCCG AATCACCCAG 
CCACTGGTAA AGAAATGGCG AGCAGCAAAG AAAGGAAAAG TCCATGAAGA CGCCAAAAGA 
GAAATCAGCT TGATATTTTC ATCTCCTGCT TGTTTGACTG CAAGTTTTTT GAAGAAAAGA 
GATGCTGGAG AGAATAATTT CATTAACGTG GACTTAAAAC TGGCAAGGGA TGTCTTCAAG 
ATGTTAACAG CAGAGAAATG GATTTCCTCC TTGATAACCA CTTGTCTTGA GGAGACTCTG 
CTTAGAGGTC TTCCATACAG CTCTCCACAT CAAGAAGCTC TATTAGTTTT TCTTTTGCTC 
CCGGAATGTT CCATTATGCA GGATCCCAAG AACTGGAAGA CACTGGCATT TGAATTTGCA 
AGCGCTATTC ATAAAATGAG GTCAAAGTCA TTAACATTCC TGAGAAACTG GTGGGCATCT 
TTGGAAGTTT CTTCTCTGAA CTCACTGGTC CAGATGCTAA AGAAAACCAT CGTCTCTCAA 
CTGCATAATG AAGTTCCAAT GATGAAGGAT ACGTCTAACC TTAAAGTTTC TTTAGAAATG 
ATGAAAGAAG TGCAAAAGAT ACTAGAGAAA ACCAACTCTC ATCTTCATAA TGAAGTTCCA 
AAGATGGAGG ATACGTCTAA TCTTAAAGTT CTCTTAGAAG TGATGAAAGA AGTGCATGAG 
GCTAACTGTC AACTTCCAGA AAATGCTTTC ATCATGAATG ACATCTTTAA CTTCAATACA 
GAAGAAGGAA TAATGGCCAA ACAGCAAATG TTCTTTATAA AATACTATTC TATGGTTTTC 
AATGATTTTC CATTTATCCT TGATTTGCCA TCCAAAATTA TATTAATGAA ACATGACTCA 
TCTGTAAAGC TGTCGGATGA AGTCAAAGTC GCTGCATCTC CAGAAAAGAT GGGATGCCCG 
TTCTTTATAC TGAAAGTCAG ACGAAGTCAT CTGGTGGAAG ACACCTTGCG TCAATTAAGA 
CAAGCTGAAG ATTTTGACCT TAGGAAGACA CTATCGGTCG GATTTATTAA TGAAATTCGT 
CCTGAGGGTG GGGGAGTAAG TTCTGAGTTC TTCCACTGTA TATTTGAAGA GATGACAGAC 
CCCAAATATG AAATGTTCAT GTATCCTGAA AATGGTTCCA ACATGTGGTT TCCAGTCAAT 
CCCAAGTTTG AGAAGAGCAG ATATTTCCTC TTTGGGATCT TATGTGGACT CTCCCTAAAT 
AATTTAAATG TTATCAACCT CTCTTTCCCA CTGGCTTTAT ATAAAAAACT TTTGGAGCAA 
AAGCCATCTT TGGAAGATTT AAAAGACCTT AGTCTTCTCC TGGGGAGGAA TTTGCAAGAA 
GTTCTAAATT GTGAGGCTGG TGTTATTGAA GAACTTCACA TGTATTTTTC TATATACTGG 
GATCAAAGAG ATGTTGATTT AATTCCAGAT GGGATCTCTG TGCCTGTGAA TGAAACAAAC 
AAGAGAGACT ATGTGTCCAA GTGTGTTGAT TACATTTTTA ACATTTCTAT AAAGACAATT 
TATGACGAAT TTCATAGAGG ATTTTACAAG GTGTGTAACC GGGACAGTAT TAGGCATTTC 
CAGCCTGAAG AACTAATGGC AGCAATAATT GGAAACCCTA CTTGTGACTG GAAACAATTT 
GAAAATAATT CAAAGTATGA AAATGGATAT TCCAAATCAC ACCCAACTAT TCTACTGTTT 
TGGAAGGCTT TCCATGAACT AACTTTGGAT GAAAAGAAAA AGTTCCTCTT GTTTCTTACA 
GGATGTGATA GGCTACATGT AAAAGGCCTA CAGAATGAAG GAATAAGATT CCGGTGTCCT 
GAAGTTTTCA GTGAAAGAGA TAACCCAAGA TCATTGACAT GTCACAGTAT TCTAGACCTG 
CCTAAGTATT CCACAATGAG AAGGATGAAG GAAGCACTTC AGGTTGCAAT CAACAATAAC 
AAGGGGTTTA TCTCACCGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761185.1
CDS	240..3323
Translation	MYFTWAPGRRQPRRLEAGTGLKLLKAASGERHSLLLFSNHRVYSCGDNSRGQLGQKSPQSTKRPEPIQALSTVHIDLVSCGKEHSVAVCHQGRVFTWGAGSEGQLGIGESKEISFMPTKINSLAGIKIIQVSCGHYHSLALSEDGQVFSWGSNRQGQLGLGNNLCSQASPQKVKSLEGIPLAQVAAGGTHSFALSLMGTSFGWGNNRSGQLALSGNSAKEQIYKPHSIGALKTLNVVYISCGYEHTSVLTEDGQVFTFGGSSSEQLQHSPRSGRGGPQLIEGIGGHVSQIECGSYHTIAYVYTTGQVVSLGRGPSCTSNATHQEAACSDVTCLLSAKDLVDIEVKDIFAGAHANFVTTCRHTRSTSVSMKILPEIIRITQPLVKKWRAAKKGKVHEDAKREISLIFSSPACLTASFLKKRDAGENNFINVDLKLARDVFKMLTAEKWISSLITTCLEETLLRGLPYSSPHQEALLVFLLLPECSIMQDPKNWKTLAFEFASAIHKMRSKSLTFLRNWWASLEVSSLNSLVQMLKKTIVSQLHNEVPMMKDTSNLKVSLEMMKEVQKILEKTNSHLHNEVPKMEDTSNLKVLLEVMKEVHEANCQLPENAFIMNDIFNFNTEEGIMAKQQMFFIKYYSMVFNDFPFILDLPSKIILMKHDSSVKLSDEVKVAASPEKMGCPFFILKVRRSHLVEDTLRQLRQAEDFDLRKTLSVGFINEIRPEGGGVSSEFFHCIFEEMTDPKYEMFMYPENGSNMWFPVNPKFEKSRYFLFGILCGLSLNNLNVINLSFPLALYKKLLEQKPSLEDLKDLSLLLGRNLQEVLNCEAGVIEELHMYFSIYWDQRDVDLIPDGISVPVNETNKRDYVSKCVDYIFNISIKTIYDEFHRGFYKVCNRDSIRHFQPEELMAAIIGNPTCDWKQFENNSKYENGYSKSHPTILLFWKAFHELTLDEKKKFLLFLTGCDRLHVKGLQNEGIRFRCPEVFSERDNPRSLTCHSILDLPKYSTMRRMKEALQVAINNNKGFISPE

Target ORF information:

RefSeq Version	XM_008762963.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (Herc6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762963.2

ORF Insert Sequence:

ATGTACTTCA CCTGGGCTCC AGGAAGGCGG CAGCCTCGGC GCCTGGAGGC AGGAACTGGT 
CTTAAGCTCT TGAAGGCGGC CAGCGGGGAA CGCCACTCGC TGCTACTGTT CAGCAACCAC 
AGGGTGTACT CCTGCGGGGA CAACAGCAGG GGCCAGCTGG GCCAGAAGAG TCCGCAGAGC 
ACCAAACGGC CAGAACCAAT TCAGGCACTA AGTACTGTAC ACATTGACCT GGTGAGCTGT 
GGGAAGGAGC ACTCAGTGGC TGTCTGCCAC CAAGGAAGGG TCTTCACATG GGGAGCCGGT 
TCTGAAGGGC AGCTGGGGAT TGGAGAATCT AAGGAAATAA GCTTCATGCC TACGAAAATA 
AACTCTCTGG CTGGCATAAA AATAATCCAA GTTTCCTGTG GACACTACCA CTCTTTGGCA 
TTATCAGAAG ATGGCCAAGT GTTTTCATGG GGAAGTAACA GACAAGGACA ACTGGGCCTG 
GGGAATAATT TATGTTCCCA AGCCAGCCCA CAGAAGGTGA AGTCCCTGGA GGGGATCCCA 
CTGGCTCAAG TAGCTGCAGG TGGCACACAC AGTTTTGCTC TGTCTCTCAT GGGGACTTCA 
TTTGGCTGGG GGAACAACAG ATCTGGGCAG CTGGCCCTCA GTGGGAACAG TGCTAAAGAG 
CAAATCTACA AGCCTCATTC AATTGGTGCC CTGAAGACTC TCAATGTGGT TTATATTAGC 
TGTGGTTATG AGCACACTTC AGTGCTTACC GAGGATGGAC AAGTGTTTAC ATTTGGAGGC 
AGTAGCTCTG AACAGCTACA GCACAGCCCC AGGAGTGGGA GAGGAGGTCC ACAACTCATA 
GAAGGGATTG GCGGCCATGT TTCACAGATA GAATGTGGAA GTTATCACAC CATTGCATAT 
GTCTACACCA CTGGTCAGGT GGTATCTTTA GGCCGTGGAC CAAGTTGCAC AAGCAACGCA 
ACTCATCAGG AGGCTGCTTG CTCTGACGTT ACCTGCTTGC TATCTGCCAA GGACCTTGTG 
GATATTGAAG TCAAAGACAT TTTTGCTGGA GCACACGCCA ACTTTGTGAC AACCTGCCGG 
CATACTAGGT CCACAAGTGT TTCCATGAAA ATCCTGCCAG AAATTATCCG AATCACCCAG 
CCACTGGTAA AGAAATGGCG AGCAGCAAAG AAAGGAAAAG TCCATGAAGA CGCCAAAAGA 
GAAATCAGCT TGATATTTTC ATCTCCTGCT TGTTTGACTG CAAGTTTTTT GAAGAAAAGA 
GATGCTGGAG AGAATAATTT CATTAACGTG GACTTAAAAC TGGCAAGGGA TGTCTTCAAG 
ATGTTAACAG CAGAGAAATG GATTTCCTCC TTGATAACCA CTTGTCTTGA GGAGACTCTG 
CTTAGAGGTC TTCCATACAG CTCTCCACAT CAAGAAGCTC TATTAGTTTT TCTTTTGCTC 
CCGGAATGTT CCATTATGCA GGATCCCAAG AACTGGAAGA CACTGGCATT TGAATTTGCA 
AGCGCTATTC ATAAAATGAG GTCAAAGTCA TTAACATTCC TGAGAAACTG GTGGGCATCT 
TTGGAAGTTT CTTCTCTGAA CTCACTGGTC CAGATGCTAA AGAAAACCAT CGTCTCTCAA 
CTGCATAATG AAGTTCCAAT GATGAAGGAT ACGTCTAACC TTAAAGTTTC TTTAGAAATG 
ATGAAAGAAG TGCAAAAGAT ACTAGAGAAA ACCAACTCTC ATCTTCATAA TGAAGTTCCA 
AAGATGGAGG ATACGTCTAA TCTTAAAGTT CTCTTAGAAG TGATGAAAGA AGTGCATGAG 
GCTAACTGTC AACTTCCAGA AAATGCTTTC ATCATGAATG ACATCTTTAA CTTCAATACA 
GAAGAAGGAA TAATGGCCAA ACAGCAAATG TTCTTTATAA AATACTATTC TATGGTTTTC 
AATGATTTTC CATTTATCCT TGATTTGCCA TCCAAAATTA TATTAATGAA ACATGACTCA 
TCTGTAAAGC TGTCGGATGA AGTCAAAGTC GCTGCATCTC CAGAAAAGAT GGGATGCCCG 
TTCTTTATAC TGAAAGTCAG ACGAAGTCAT CTGGTGGAAG ACACCTTGCG TCAATTAAGA 
CAAGCTGAAG ATTTTGACCT TAGGAAGACA CTATCGGTCG GATTTATTAA TGAAATTCGT 
CCTGAGGGTG GGGGAGTAAG TTCTGAGTTC TTCCACTGTA TATTTGAAGA GATGACAGAC 
CCCAAATATG AAATGTTCAT GTATCCTGAA AATGGTTCCA ACATGTGGTT TCCAGTCAAT 
CCCAAGTTTG AGAAGAGCAG ATATTTCCTC TTTGGGATCT TATGTGGACT CTCCCTAAAT 
AATTTAAATG TTATCAACCT CTCTTTCCCA CTGGCTTTAT ATAAAAAACT TTTGGAGCAA 
AAGCCATCTT TGGAAGATTT AAAAGACCTT AGTCTTCTCC TGGGGAGGAA TTTGCAAGAA 
GTTCTAAATT GTGAGGCTGG TGTTATTGAA GAACTTCACA TGTATTTTTC TATATACTGG 
GATCAAAGAG ATGTTGATTT AATTCCAGAT GGGATCTCTG TGCCTGTGAA TGAAACAAAC 
AAGAGAGACT ATGTGTCCAA GTGTGTTGAT TACATTTTTA ACATTTCTAT AAAGACAATT 
TATGACGAAT TTCATAGAGG ATTTTACAAG GTGTGTAACC GGGACAGTAT TAGGCATTTC 
CAGCCTGAAG AACTAATGGC AGCAATAATT GGAAACCCTA CTTGTGACTG GAAACAATTT 
GAAAATAATT CAAAGTATGA AAATGGATAT TCCAAATCAC ACCCAACTAT TCTACTGTTT 
TGGAAGGCTT TCCATGAACT AACTTTGGAT GAAAAGAAAA AGTTCCTCTT GTTTCTTACA 
GGATGTGATA GGCTACATGT AAAAGGCCTA CAGAATGAAG GAATAAGATT CCGGTGTCCT 
GAAGTTTTCA GTGAAAGAGA TAACCCAAGA TCATTGACAT GTCACAGTAT TCTAGACCTG 
CCTAAGTATT CCACAATGAG AAGGATGAAG GAAGCACTTC AGGTTGCAAT CAACAATAAC 
AAGGGGTTTA TCTCACCGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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Herc6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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