HERC6 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HERC6
Entrez Gene ID	527520
Full Name	HECT and RLD domain containing E3 ubiquitin protein ligase family member 6
General protein information	Preferred Names HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 Names probable E3 ubiquitin-protein ligase HERC6 hect domain and RLD 6
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010806029.2	XP_010804331.1	probable E3 ubiquitin-protein ligase HERC6 isoform X1
XM_010806029.3	XP_010804331.2	probable E3 ubiquitin-protein ligase HERC6 isoform X1
XM_024993241.1	XP_024849009.1	probable E3 ubiquitin-protein ligase HERC6 isoform X2
NM_001192644.2	NP_001179573.2	probable E3 ubiquitin-protein ligase HERC6
NM_001192644.1	NP_001179573.1	probable E3 ubiquitin-protein ligase HERC6
NM_001192644.3	NP_001179573.3	probable E3 ubiquitin-protein ligase HERC6

Pathways from BioSystems

Homologies

	HERC6	XP_002804182.1
Bos taurus (cattle)	HERC6	NP_001179573.1
Homo sapiens (human)	HERC6	NP_060382.3
Canis lupus familiaris (dog)	HERC6	XP_851549.1
Pan troglodytes (chimpanzee)	HERC6	XP_001160851.1
Xenopus tropicalis (tropical clawed frog)	herc6	XP_002938670.2
Mus musculus (house mouse)	Herc6	NP_080268.1
Rattus norvegicus (Norway rat)	Herc6	XP_006236645.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HERC6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HERC6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa235329	XM_024993241.1 Latest version!	Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa216171	NM_001192644.2 Latest version!	Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa235328	XM_010806029.3 Latest version!	Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa01694	NM_001192644.3 Latest version!	Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa51406	XM_010806029.2 Latest version!	Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa01694	NM_001192644.1	Bos taurus hect domain and RLD 6 (HERC6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HERC6 ( XM_024993241.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849009.1 Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X2, mRNA.

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CloneID	OBa235329
Clone ID Related Accession (Same CDS sequence)	XM_024993241.1
Accession Version	XM_024993241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGAAGG ATACTAGTTC CACCAGTGTT CCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGACAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATGATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
GATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CGCATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CATTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTATAT AACTCTGGTG 
GTTCCATTTG CAGAGGCTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCGG 
TGTTGGACGT CTTTGCAAGA ATCTTCTCTC AGCTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATCTCTC AAATGGTTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG ATACCTGCAG AAAACTTCAG TCCGATTATT 
TTCAGTGATT TTCCATTTGT CTTTAATTTG ATGTCCAAAA TTAAATTATT GCAAGCTGAT 
TCCCAAATAA GAGTGCTGAA ATCTGAAGTG AACAACTACA TGGATTTTAG GGGAATAATT 
CTGCAAAGAA GGGATGAATC TCCTTTATTT ACACTTAGCG TCAGACGAAG TCACTTAGTT 
GAAGATGCTC TATGTCAGTT AAGCCAAGCT GAAGACACTG ACCTCCAGAA AATATTAGTG 
GTTGAATTTA TTAAAGAAAT TCGTTCTGGA GGGGAAGGGG TGAAGTCTGA GTTTTTTCAC 
TGTATATTTG AATCTATGAC CAAGGAAGAA TATGGAATGT TCATGTATCC CGAAGAGGAT 
TCCTACATGT GGTTTCCTGT CAAACCTAAA TTTGAGAAGA AGATGTATTT CCTCTTTGGG 
ATGCTGTGTG GACTCTCCCT ATATAATTTT AATGTTGTCT ACCTCCCTTT CCCACTGGCT 
CTGTTTAAGA AACTTCTGGA CCAGGAGCCA TCATTGGAAG ATTTAAAAGA ACTCAGTCCT 
TCTTTTGGAA AGTGCTTGCA AGAAGTTCTA AATGATGATG CTAATGACAT TAAAGAAGAA 
TTTGGCCTAC GTTTTTCTAT ACACTGGGAC CAAAATGATG TTGATTTAAT TCCAAATGGG 
ATTTCTGTAT TTGTAGACCA AAGCAACAAG AAAGACTATG TTTCTAAGTG TGTTGATTAT 
GTTTTAAACA CCTCTGTAAA GGCAGTTTAT GAGGAATTTC AGAGAGGATT TTATAAACTT 
TTTGACAAAG AGATACTTAA ACATTTCAAG CCTGAAGAAC TAATGAGAGC AATAATTGGA 
AATACTGACT ATGACTGGGA ACAATTTGAA AAGAATTCTA TTTATGAGCA AGGATACCAT 
GAGTCACATC CTACTATACT GATGTTTTGG AAAGCTTTCC ATAGTTTAAC TTTGGATGAA 
AAGAGAAAAT TCCTCTTTTT TCTTACAGGA AATGATAGAC TACATGTAAA AGGCATACAG 
AAACCAGGAA TCCGGTTTCG CTGTCCTGAG ACTTGCAGTG AAAGCGATTT CCCAAAATCA 
CTGACATGTC ATAATATTCT GGAACTCCCT GAATATTCTA CAATGAAAAA AGTGAAGGAA 
GCACTTCAAA GAGCCATCAA CAGCAACAAA GGATTTATCT CAGCCACAGT CACAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849009.1
CDS	123..3062
Translation	MYFCWRAGGRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLNVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGEFKETTFIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGTNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGHHSFALSLSGTSFGWGSNSAGQLALSGNNAPVQRYKPVSIGALKTLGVVSISCGYKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSDHTLAYIYTTGQVVSFGRGPGCASRSPHPGAESSDISCLVSAKDLADVQVKHIFAGTYANFVTTSQKDTSSTSVPRKTLPEISRINQSLTEKWMAVAKRSIEDEVAKSEIRVIFSSPACLTASFLKKREPGEMISIDVDLEMARDTFKKLTEKEWISSMITACLKDNLLGALPCHSSHQEALSVFLLLPECPVMLDSRNYITLVVPFAEAIRKMTNQSSKVLKRCWTSLQESSLSSLVQMLKTAIISQMVCWTETVQSNHNVKTLLEVMKDVYKIPAENFSPIIFSDFPFVFNLMSKIKLLQADSQIRVLKSEVNNYMDFRGIILQRRDESPLFTLSVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSGGEGVKSEFFHCIFESMTKEEYGMFMYPEEDSYMWFPVKPKFEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLDQEPSLEDLKELSPSFGKCLQEVLNDDANDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVLNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHSLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFRCPETCSESDFPKSLTCHNILELPEYSTMKKVKEALQRAINSNKGFISATVTG

Target ORF information:

RefSeq Version	XM_024993241.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993241.1

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGAAGG ATACTAGTTC CACCAGTGTT CCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGACAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATGATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
GATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CGCATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CATTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTATAT AACTCTGGTG 
GTTCCATTTG CAGAGGCTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCGG 
TGTTGGACGT CTTTGCAAGA ATCTTCTCTC AGCTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATCTCTC AAATGGTTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG ATACCTGCAG AAAACTTCAG TCCGATTATT 
TTCAGTGATT TTCCATTTGT CTTTAATTTG ATGTCCAAAA TTAAATTATT GCAAGCTGAT 
TCCCAAATAA GAGTGCTGAA ATCTGAAGTG AACAACTACA TGGATTTTAG GGGAATAATT 
CTGCAAAGAA GGGATGAATC TCCTTTATTT ACACTTAGCG TCAGACGAAG TCACTTAGTT 
GAAGATGCTC TATGTCAGTT AAGCCAAGCT GAAGACACTG ACCTCCAGAA AATATTAGTG 
GTTGAATTTA TTAAAGAAAT TCGTTCTGGA GGGGAAGGGG TGAAGTCTGA GTTTTTTCAC 
TGTATATTTG AATCTATGAC CAAGGAAGAA TATGGAATGT TCATGTATCC CGAAGAGGAT 
TCCTACATGT GGTTTCCTGT CAAACCTAAA TTTGAGAAGA AGATGTATTT CCTCTTTGGG 
ATGCTGTGTG GACTCTCCCT ATATAATTTT AATGTTGTCT ACCTCCCTTT CCCACTGGCT 
CTGTTTAAGA AACTTCTGGA CCAGGAGCCA TCATTGGAAG ATTTAAAAGA ACTCAGTCCT 
TCTTTTGGAA AGTGCTTGCA AGAAGTTCTA AATGATGATG CTAATGACAT TAAAGAAGAA 
TTTGGCCTAC GTTTTTCTAT ACACTGGGAC CAAAATGATG TTGATTTAAT TCCAAATGGG 
ATTTCTGTAT TTGTAGACCA AAGCAACAAG AAAGACTATG TTTCTAAGTG TGTTGATTAT 
GTTTTAAACA CCTCTGTAAA GGCAGTTTAT GAGGAATTTC AGAGAGGATT TTATAAACTT 
TTTGACAAAG AGATACTTAA ACATTTCAAG CCTGAAGAAC TAATGAGAGC AATAATTGGA 
AATACTGACT ATGACTGGGA ACAATTTGAA AAGAATTCTA TTTATGAGCA AGGATACCAT 
GAGTCACATC CTACTATACT GATGTTTTGG AAAGCTTTCC ATAGTTTAAC TTTGGATGAA 
AAGAGAAAAT TCCTCTTTTT TCTTACAGGA AATGATAGAC TACATGTAAA AGGCATACAG 
AAACCAGGAA TCCGGTTTCG CTGTCCTGAG ACTTGCAGTG AAAGCGATTT CCCAAAATCA 
CTGACATGTC ATAATATTCT GGAACTCCCT GAATATTCTA CAATGAAAAA AGTGAAGGAA 
GCACTTCAAA GAGCCATCAA CAGCAACAAA GGATTTATCT CAGCCACAGT CACAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC6 ( NM_001192644.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179573.2 Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), mRNA.

Price & Availability↑

CloneID	OBa216171
Clone ID Related Accession (Same CDS sequence)	NM_001192644.2
Accession Version	NM_001192644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase HERC6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02017014.1. On Jun 30, 2017 this sequence version replaced NM_001192644.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGGATA CTAGTTCCAC CAGTGTTCCC AGGAAAACCC TGCCAGAAAT AAGCCGAATT 
AACCAGTCTC TGACAGAAAA ATGGATGGCA GTGGCAAAAA GAAGTATAGA AGATGAAGTG 
GCTAAGAGTG AAATTAGAGT GATATTTTCA TCTCCTGCTT GTCTGACTGC AAGTTTTTTA 
AAGAAGAGAG AACCTGGAGA AATGATTTCC ATTGATGTGG ACTTAGAAAT GGCAAGAGAT 
ACCTTCAAGA AGTTAACAGA AAAAGAATGG ATTTCTTCGA TGATAACCGC ATGTCTCAAG 
GATAATCTGC TTGGGGCTCT TCCATGTCAT TCTTCACACC AAGAAGCTTT ATCAGTCTTC 
CTTCTGCTCC CAGAGTGTCC TGTCATGCTT GATTCCAGGA ACTATATAAC TCTGGTGGTT 
CCATTTGCAG AGGCTATTCG TAAAATGACC AATCAATCTT CAAAAGTCCT GAAGCGGTGT 
TGGACGTCTT TGCAAGAATC TTCTCTCAGC TCACTTGTCC AGATGCTGAA AACTGCCATC 
ATCTCTCAAA TGGTTTGCTG GACTGAAACT GTTCAGAGTA ACCACAATGT TAAAACTCTT 
CTGGAAGTGA TGAAAGACGT GTATAAGGTA AACAAAACTA ACTGTCGATT ATCAGAAGAT 
ACGTTCCACA TAAATGAAGT CTCCTTTTTG TTGAACTTTT ATGAAGATAG AATAAGAGTG 
ATTAATAGGG AAAAAAACCT GATACCTGCA GAAAACTTCA GTCCGATTAT TTTCAGTGAT 
TTTCCATTTG TCTTTAATTT GATGTCCAAA ATTAAATTAT TGCAAGCTGA TTCCCAAATA 
AGAGTGCTGA AATCTGAAGT GAACAACTAC ATGGATTTTA GGGGAATAAT TCTGCAAAGA 
AGGGATGAAT CTCCTTTATT TACACTTAGC GTCAGACGAA GTCACTTAGT TGAAGATGCT 
CTATGTCAGT TAAGCCAAGC TGAAGACACT GACCTCCAGA AAATATTAGT GGTTGAATTT 
ATTAAAGAAA TTCGTTCTGG AGGGGAAGGG GTGAAGTCTG AGTTTTTTCA CTGTATATTT 
GAATCTATGA CCAAGGAAGA ATATGGAATG TTCATGTATC CCGAAGAGGA TTCCTACATG 
TGGTTTCCTG TCAAACCTAA ATTTGAGAAG AAGATGTATT TCCTCTTTGG GATGCTGTGT 
GGACTCTCCC TATATAATTT TAATGTTGTC TACCTCCCTT TCCCACTGGC TCTGTTTAAG 
AAACTTCTGG ACCAGGAGCC ATCATTGGAA GATTTAAAAG AACTCAGTCC TTCTTTTGGA 
AAGTGCTTGC AAGAAGTTCT AAATGATGAT GTTAATGACA TTAAAGAAGA ATTTGGCCTA 
CGTTTTTCTA TACACTGGGA CCAAAATGAT GTTGATTTAA TTCCAAATGG GATTTCTGTA 
TTTGTAGACC AAAGCAACAA GAAAGACTAT GTTTCTAAGT GTGTTGATTA TGTTTTAAAC 
ACCTCTGTAA AGGCAGTTTA TGAGGAATTT CAGAGAGGAT TTTATAAACT TTTTGACAAA 
GAGATACTTA AACATTTCAA GCCTGAAGAA CTAATGAGAG CAATAATTGG AAATACTGAC 
TATGACTGGG AACAATTTGA AAAGAATTCT ATTTATGAGC AAGGATACCA TGAGTCACAT 
CCTACTATAC TGATGTTTTG GAAAGCTTTC CATAGTTTAA CTTTGGATGA AAAGAGAAAA 
TTCCTCTTTT TTCTTACAGG AAATGATAGA CTACATGTAA AAGGCATACA GAAACCAGGA 
ATCCGGTTTC GCTGTCCTGA GACTTGCAGT GAAAGCGATT TCCCAAAATC ACTGACATGT 
CATAATATTC TGGAACTCCC TGAATATTCT ACAATGAAAA AAGTGAAGGA AGCACTTCAA 
AGAGCCATCA ACAGCAACAA AGGATTTATC TCAGCCACAG TCACAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179573.2
CDS	121..3171
Translation	MYFCWRAGGRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLNVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGEFKETTFIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGTNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGHHSFALSLSGTSFGWGSNSAGQLALSGNNAPVQRYKPVSIGALKTLGVVSISCGYKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSDHTLAYIYTTGQVVSFGRGPGCASRSPHPGAESSDISCLVSAKDLADVQVKHIFAGTYANFVTTSQDTSSTSVPRKTLPEISRINQSLTEKWMAVAKRSIEDEVAKSEIRVIFSSPACLTASFLKKREPGEMISIDVDLEMARDTFKKLTEKEWISSMITACLKDNLLGALPCHSSHQEALSVFLLLPECPVMLDSRNYITLVVPFAEAIRKMTNQSSKVLKRCWTSLQESSLSSLVQMLKTAIISQMVCWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLSEDTFHINEVSFLLNFYEDRIRVINREKNLIPAENFSPIIFSDFPFVFNLMSKIKLLQADSQIRVLKSEVNNYMDFRGIILQRRDESPLFTLSVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSGGEGVKSEFFHCIFESMTKEEYGMFMYPEEDSYMWFPVKPKFEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLDQEPSLEDLKELSPSFGKCLQEVLNDDVNDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVLNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHSLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFRCPETCSESDFPKSLTCHNILELPEYSTMKKVKEALQRAINSNKGFISATVTG

Target ORF information:

RefSeq Version	NM_001192644.2
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192644.2

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGGATA CTAGTTCCAC CAGTGTTCCC AGGAAAACCC TGCCAGAAAT AAGCCGAATT 
AACCAGTCTC TGACAGAAAA ATGGATGGCA GTGGCAAAAA GAAGTATAGA AGATGAAGTG 
GCTAAGAGTG AAATTAGAGT GATATTTTCA TCTCCTGCTT GTCTGACTGC AAGTTTTTTA 
AAGAAGAGAG AACCTGGAGA AATGATTTCC ATTGATGTGG ACTTAGAAAT GGCAAGAGAT 
ACCTTCAAGA AGTTAACAGA AAAAGAATGG ATTTCTTCGA TGATAACCGC ATGTCTCAAG 
GATAATCTGC TTGGGGCTCT TCCATGTCAT TCTTCACACC AAGAAGCTTT ATCAGTCTTC 
CTTCTGCTCC CAGAGTGTCC TGTCATGCTT GATTCCAGGA ACTATATAAC TCTGGTGGTT 
CCATTTGCAG AGGCTATTCG TAAAATGACC AATCAATCTT CAAAAGTCCT GAAGCGGTGT 
TGGACGTCTT TGCAAGAATC TTCTCTCAGC TCACTTGTCC AGATGCTGAA AACTGCCATC 
ATCTCTCAAA TGGTTTGCTG GACTGAAACT GTTCAGAGTA ACCACAATGT TAAAACTCTT 
CTGGAAGTGA TGAAAGACGT GTATAAGGTA AACAAAACTA ACTGTCGATT ATCAGAAGAT 
ACGTTCCACA TAAATGAAGT CTCCTTTTTG TTGAACTTTT ATGAAGATAG AATAAGAGTG 
ATTAATAGGG AAAAAAACCT GATACCTGCA GAAAACTTCA GTCCGATTAT TTTCAGTGAT 
TTTCCATTTG TCTTTAATTT GATGTCCAAA ATTAAATTAT TGCAAGCTGA TTCCCAAATA 
AGAGTGCTGA AATCTGAAGT GAACAACTAC ATGGATTTTA GGGGAATAAT TCTGCAAAGA 
AGGGATGAAT CTCCTTTATT TACACTTAGC GTCAGACGAA GTCACTTAGT TGAAGATGCT 
CTATGTCAGT TAAGCCAAGC TGAAGACACT GACCTCCAGA AAATATTAGT GGTTGAATTT 
ATTAAAGAAA TTCGTTCTGG AGGGGAAGGG GTGAAGTCTG AGTTTTTTCA CTGTATATTT 
GAATCTATGA CCAAGGAAGA ATATGGAATG TTCATGTATC CCGAAGAGGA TTCCTACATG 
TGGTTTCCTG TCAAACCTAA ATTTGAGAAG AAGATGTATT TCCTCTTTGG GATGCTGTGT 
GGACTCTCCC TATATAATTT TAATGTTGTC TACCTCCCTT TCCCACTGGC TCTGTTTAAG 
AAACTTCTGG ACCAGGAGCC ATCATTGGAA GATTTAAAAG AACTCAGTCC TTCTTTTGGA 
AAGTGCTTGC AAGAAGTTCT AAATGATGAT GTTAATGACA TTAAAGAAGA ATTTGGCCTA 
CGTTTTTCTA TACACTGGGA CCAAAATGAT GTTGATTTAA TTCCAAATGG GATTTCTGTA 
TTTGTAGACC AAAGCAACAA GAAAGACTAT GTTTCTAAGT GTGTTGATTA TGTTTTAAAC 
ACCTCTGTAA AGGCAGTTTA TGAGGAATTT CAGAGAGGAT TTTATAAACT TTTTGACAAA 
GAGATACTTA AACATTTCAA GCCTGAAGAA CTAATGAGAG CAATAATTGG AAATACTGAC 
TATGACTGGG AACAATTTGA AAAGAATTCT ATTTATGAGC AAGGATACCA TGAGTCACAT 
CCTACTATAC TGATGTTTTG GAAAGCTTTC CATAGTTTAA CTTTGGATGA AAAGAGAAAA 
TTCCTCTTTT TTCTTACAGG AAATGATAGA CTACATGTAA AAGGCATACA GAAACCAGGA 
ATCCGGTTTC GCTGTCCTGA GACTTGCAGT GAAAGCGATT TCCCAAAATC ACTGACATGT 
CATAATATTC TGGAACTCCC TGAATATTCT ACAATGAAAA AAGTGAAGGA AGCACTTCAA 
AGAGCCATCA ACAGCAACAA AGGATTTATC TCAGCCACAG TCACAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC6 ( XM_010806029.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804331.2 Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X1, mRNA.

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CloneID	OBa235328
Clone ID Related Accession (Same CDS sequence)	XM_010806029.3
Accession Version	XM_010806029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGAAGG ATACTAGTTC CACCAGTGTT CCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGACAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATGATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
GATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CGCATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CATTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTATAT AACTCTGGTG 
GTTCCATTTG CAGAGGCTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCGG 
TGTTGGACGT CTTTGCAAGA ATCTTCTCTC AGCTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATCTCTC AAATGGTTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG GTAAACAAAA CTAACTGTCG ATTATCAGAA 
GATACGTTCC ACATAAATGA AGTCTCCTTT TTGTTGAACT TTTATGAAGA TAGAATAAGA 
GTGATTAATA GGGAAAAAAA CCTGATACCT GCAGAAAACT TCAGTCCGAT TATTTTCAGT 
GATTTTCCAT TTGTCTTTAA TTTGATGTCC AAAATTAAAT TATTGCAAGC TGATTCCCAA 
ATAAGAGTGC TGAAATCTGA AGTGAACAAC TACATGGATT TTAGGGGAAT AATTCTGCAA 
AGAAGGGATG AATCTCCTTT ATTTACACTT AGCGTCAGAC GAAGTCACTT AGTTGAAGAT 
GCTCTATGTC AGTTAAGCCA AGCTGAAGAC ACTGACCTCC AGAAAATATT AGTGGTTGAA 
TTTATTAAAG AAATTCGTTC TGGAGGGGAA GGGGTGAAGT CTGAGTTTTT TCACTGTATA 
TTTGAATCTA TGACCAAGGA AGAATATGGA ATGTTCATGT ATCCCGAAGA GGATTCCTAC 
ATGTGGTTTC CTGTCAAACC TAAATTTGAG AAGAAGATGT ATTTCCTCTT TGGGATGCTG 
TGTGGACTCT CCCTATATAA TTTTAATGTT GTCTACCTCC CTTTCCCACT GGCTCTGTTT 
AAGAAACTTC TGGACCAGGA GCCATCATTG GAAGATTTAA AAGAACTCAG TCCTTCTTTT 
GGAAAGTGCT TGCAAGAAGT TCTAAATGAT GATGCTAATG ACATTAAAGA AGAATTTGGC 
CTACGTTTTT CTATACACTG GGACCAAAAT GATGTTGATT TAATTCCAAA TGGGATTTCT 
GTATTTGTAG ACCAAAGCAA CAAGAAAGAC TATGTTTCTA AGTGTGTTGA TTATGTTTTA 
AACACCTCTG TAAAGGCAGT TTATGAGGAA TTTCAGAGAG GATTTTATAA ACTTTTTGAC 
AAAGAGATAC TTAAACATTT CAAGCCTGAA GAACTAATGA GAGCAATAAT TGGAAATACT 
GACTATGACT GGGAACAATT TGAAAAGAAT TCTATTTATG AGCAAGGATA CCATGAGTCA 
CATCCTACTA TACTGATGTT TTGGAAAGCT TTCCATAGTT TAACTTTGGA TGAAAAGAGA 
AAATTCCTCT TTTTTCTTAC AGGAAATGAT AGACTACATG TAAAAGGCAT ACAGAAACCA 
GGAATCCGGT TTCGCTGTCC TGAGACTTGC AGTGAAAGCG ATTTCCCAAA ATCACTGACA 
TGTCATAATA TTCTGGAACT CCCTGAATAT TCTACAATGA AAAAAGTGAA GGAAGCACTT 
CAAAGAGCCA TCAACAGCAA CAAAGGATTT ATCTCAGCCA CAGTCACAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804331.2
CDS	141..3194
Translation	MYFCWRAGGRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLNVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGEFKETTFIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGTNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGHHSFALSLSGTSFGWGSNSAGQLALSGNNAPVQRYKPVSIGALKTLGVVSISCGYKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSDHTLAYIYTTGQVVSFGRGPGCASRSPHPGAESSDISCLVSAKDLADVQVKHIFAGTYANFVTTSQKDTSSTSVPRKTLPEISRINQSLTEKWMAVAKRSIEDEVAKSEIRVIFSSPACLTASFLKKREPGEMISIDVDLEMARDTFKKLTEKEWISSMITACLKDNLLGALPCHSSHQEALSVFLLLPECPVMLDSRNYITLVVPFAEAIRKMTNQSSKVLKRCWTSLQESSLSSLVQMLKTAIISQMVCWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLSEDTFHINEVSFLLNFYEDRIRVINREKNLIPAENFSPIIFSDFPFVFNLMSKIKLLQADSQIRVLKSEVNNYMDFRGIILQRRDESPLFTLSVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSGGEGVKSEFFHCIFESMTKEEYGMFMYPEEDSYMWFPVKPKFEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLDQEPSLEDLKELSPSFGKCLQEVLNDDANDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVLNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHSLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFRCPETCSESDFPKSLTCHNILELPEYSTMKKVKEALQRAINSNKGFISATVTG

Target ORF information:

RefSeq Version	XM_010806029.3
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806029.3

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGAAGG ATACTAGTTC CACCAGTGTT CCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGACAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATGATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
GATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CGCATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CATTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTATAT AACTCTGGTG 
GTTCCATTTG CAGAGGCTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCGG 
TGTTGGACGT CTTTGCAAGA ATCTTCTCTC AGCTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATCTCTC AAATGGTTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG GTAAACAAAA CTAACTGTCG ATTATCAGAA 
GATACGTTCC ACATAAATGA AGTCTCCTTT TTGTTGAACT TTTATGAAGA TAGAATAAGA 
GTGATTAATA GGGAAAAAAA CCTGATACCT GCAGAAAACT TCAGTCCGAT TATTTTCAGT 
GATTTTCCAT TTGTCTTTAA TTTGATGTCC AAAATTAAAT TATTGCAAGC TGATTCCCAA 
ATAAGAGTGC TGAAATCTGA AGTGAACAAC TACATGGATT TTAGGGGAAT AATTCTGCAA 
AGAAGGGATG AATCTCCTTT ATTTACACTT AGCGTCAGAC GAAGTCACTT AGTTGAAGAT 
GCTCTATGTC AGTTAAGCCA AGCTGAAGAC ACTGACCTCC AGAAAATATT AGTGGTTGAA 
TTTATTAAAG AAATTCGTTC TGGAGGGGAA GGGGTGAAGT CTGAGTTTTT TCACTGTATA 
TTTGAATCTA TGACCAAGGA AGAATATGGA ATGTTCATGT ATCCCGAAGA GGATTCCTAC 
ATGTGGTTTC CTGTCAAACC TAAATTTGAG AAGAAGATGT ATTTCCTCTT TGGGATGCTG 
TGTGGACTCT CCCTATATAA TTTTAATGTT GTCTACCTCC CTTTCCCACT GGCTCTGTTT 
AAGAAACTTC TGGACCAGGA GCCATCATTG GAAGATTTAA AAGAACTCAG TCCTTCTTTT 
GGAAAGTGCT TGCAAGAAGT TCTAAATGAT GATGCTAATG ACATTAAAGA AGAATTTGGC 
CTACGTTTTT CTATACACTG GGACCAAAAT GATGTTGATT TAATTCCAAA TGGGATTTCT 
GTATTTGTAG ACCAAAGCAA CAAGAAAGAC TATGTTTCTA AGTGTGTTGA TTATGTTTTA 
AACACCTCTG TAAAGGCAGT TTATGAGGAA TTTCAGAGAG GATTTTATAA ACTTTTTGAC 
AAAGAGATAC TTAAACATTT CAAGCCTGAA GAACTAATGA GAGCAATAAT TGGAAATACT 
GACTATGACT GGGAACAATT TGAAAAGAAT TCTATTTATG AGCAAGGATA CCATGAGTCA 
CATCCTACTA TACTGATGTT TTGGAAAGCT TTCCATAGTT TAACTTTGGA TGAAAAGAGA 
AAATTCCTCT TTTTTCTTAC AGGAAATGAT AGACTACATG TAAAAGGCAT ACAGAAACCA 
GGAATCCGGT TTCGCTGTCC TGAGACTTGC AGTGAAAGCG ATTTCCCAAA ATCACTGACA 
TGTCATAATA TTCTGGAACT CCCTGAATAT TCTACAATGA AAAAAGTGAA GGAAGCACTT 
CAAAGAGCCA TCAACAGCAA CAAAGGATTT ATCTCAGCCA CAGTCACAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC6 ( NM_001192644.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179573.1 Bos taurus hect domain and RLD 6 (HERC6), mRNA.

Price & Availability↑

CloneID	OBa01694
Clone ID Related Accession (Same CDS sequence)	NM_001192644.1 , NM_001192644.3
Accession Version	NM_001192644.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-10-23
Organism	Bos taurus(Cattle)
Product	probable E3 ubiquitin-protein ligase HERC6
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03039442.1, AAFC03039444.1, AAFC03124174.1 and AAFC03039445.1. On Jul 17, 2010 this sequence version replaced XM_605913.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGGATA CTAGTTCCAC CAGTGTTCCC AGGAAAACCC TGCCAGAAAT AAGCCGAATT 
AACCAGTCTC TGACAGAAAA ATGGATGGCA GTGGCAAAAA GAAGTATAGA AGATGAAGTG 
GCTAAGAGTG AAATTAGAGT GATATTTTCA TCTCCTGCTT GTCTGACTGC AAGTTTTTTA 
AAGAAGAGAG AACCTGGAGA AATGATTTCC ATTGATGTGG ACTTAGAAAT GGCAAGAGAT 
ACCTTCAAGA AGTTAACAGA AAAAGAATGG ATTTCTTCGA TGATAACCGC ATGTCTCAAG 
GATAATCTGC TTGGGGCTCT TCCATGTCAT TCTTCACACC AAGAAGCTTT ATCAGTCTTC 
CTTCTGCTCC CAGAGTGTCC TGTCATGCTT GATTCCAGGA ACTATATAAC TCTGGTGGTT 
CCATTTGCAG AGGCTATTCG TAAAATGACC AATCAATCTT CAAAAGTCCT GAAGCGGTGT 
TGGACGTCTT TGCAAGAATC TTCTCTCAGC TCACTTGTCC AGATGCTGAA AACTGCCATC 
ATCTCTCAAA TGGTTTGCTG GACTGAAACT GTTCAGAGTA ACCACAATGT TAAAACTCTT 
CTGGAAGTGA TGAAAGACGT GTATAAGGTA AACAAAACTA ACTGTCGATT ATCAGAAGAT 
ACGTTCCACA TAAATGAAGT CTCCTTTTTG TTGAACTTTT ATGAAGATAG AATAAGAGTG 
ATTAATAGGG AAAAAAACCT GATACCTGCA GAAAACTTCA GTCCGATTAT TTTCAGTGAT 
TTTCCATTTG TCTTTAATTT GATGTCCAAA ATTAAATTAT TGCAAGCTGA TTCCCAAATA 
AGAGTGCTGA AATCTGAAGT GAACAACTAC ATGGATTTTA GGGGAATAAT TCTGCAAAGA 
AGGGATGAAT CTCCTTTATT TACACTTAGC GTCAGACGAA GTCACTTAGT TGAAGATGCT 
CTATGTCAGT TAAGCCAAGC TGAAGACACT GACCTCCAGA AAATATTAGT GGTTGAATTT 
ATTAAAGAAA TTCGTTCTGG AGGGGAAGGG GTGAAGTCTG AGTTTTTTCA CTGTATATTT 
GAATCTATGA CCAAGGAAGA ATATGGAATG TTCATGTATC CCGAAGAGGA TTCCTACATG 
TGGTTTCCTG TCAAACCTAA ATTTGAGAAG AAGATGTATT TCCTCTTTGG GATGCTGTGT 
GGACTCTCCC TATATAATTT TAATGTTGTC TACCTCCCTT TCCCACTGGC TCTGTTTAAG 
AAACTTCTGG ACCAGGAGCC ATCATTGGAA GATTTAAAAG AACTCAGTCC TTCTTTTGGA 
AAGTGCTTGC AAGAAGTTCT AAATGATGAT GCTAATGACA TTAAAGAAGA ATTTGGCCTA 
CGTTTTTCTA TACACTGGGA CCAAAATGAT GTTGATTTAA TTCCAAATGG GATTTCTGTA 
TTTGTAGACC AAAGCAACAA GAAAGACTAT GTTTCTAAGT GTGTTGATTA TGTTTTAAAC 
ACCTCTGTAA AGGCAGTTTA TGAGGAATTT CAGAGAGGAT TTTATAAACT TTTTGACAAA 
GAGATACTTA AACATTTCAA GCCTGAAGAA CTAATGAGAG CAATAATTGG AAATACTGAC 
TATGACTGGG AACAATTTGA AAAGAATTCT ATTTATGAGC AAGGATACCA TGAGTCACAT 
CCTACTATAC TGATGTTTTG GAAAGCTTTC CATAGTTTAA CTTTGGATGA AAAGAGAAAA 
TTCCTCTTTT TTCTTACAGG AAATGATAGA CTACATGTAA AAGGCATACA GAAACCAGGA 
ATCCGGTTTC GCTGTCCTGA GACTTGCAGT GAAAGCGATT TCCCAAAATC ACTGACATGT 
CATAATATTC TGGAACTCCC TGAATATTCT ACAATGAAAA AAGTGAAGGA AGCACTTCAA 
AGAGCCATCA ACAGCAACAA AGGATTTATC TCAGCCACAG TCACAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179573.1
CDS	121..3171
Translation	MYFCWRAGGRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLNVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGEFKETTFIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGTNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGHHSFALSLSGTSFGWGSNSAGQLALSGNNAPVQRYKPVSIGALKTLGVVSISCGYKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSDHTLAYIYTTGQVVSFGRGPGCASRSPHPGAESSDISCLVSAKDLADVQVKHIFAGTYANFVTTSQDTSSTSVPRKTLPEISRINQSLTEKWMAVAKRSIEDEVAKSEIRVIFSSPACLTASFLKKREPGEMISIDVDLEMARDTFKKLTEKEWISSMITACLKDNLLGALPCHSSHQEALSVFLLLPECPVMLDSRNYITLVVPFAEAIRKMTNQSSKVLKRCWTSLQESSLSSLVQMLKTAIISQMVCWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLSEDTFHINEVSFLLNFYEDRIRVINREKNLIPAENFSPIIFSDFPFVFNLMSKIKLLQADSQIRVLKSEVNNYMDFRGIILQRRDESPLFTLSVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSGGEGVKSEFFHCIFESMTKEEYGMFMYPEEDSYMWFPVKPKFEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLDQEPSLEDLKELSPSFGKCLQEVLNDDANDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVLNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHSLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFRCPETCSESDFPKSLTCHNILELPEYSTMKKVKEALQRAINSNKGFISATVTG

Target ORF information:

RefSeq Version	NM_001192644.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus hect domain and RLD 6 (HERC6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192644.1

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGGATA CTAGTTCCAC CAGTGTTCCC AGGAAAACCC TGCCAGAAAT AAGCCGAATT 
AACCAGTCTC TGACAGAAAA ATGGATGGCA GTGGCAAAAA GAAGTATAGA AGATGAAGTG 
GCTAAGAGTG AAATTAGAGT GATATTTTCA TCTCCTGCTT GTCTGACTGC AAGTTTTTTA 
AAGAAGAGAG AACCTGGAGA AATGATTTCC ATTGATGTGG ACTTAGAAAT GGCAAGAGAT 
ACCTTCAAGA AGTTAACAGA AAAAGAATGG ATTTCTTCGA TGATAACCGC ATGTCTCAAG 
GATAATCTGC TTGGGGCTCT TCCATGTCAT TCTTCACACC AAGAAGCTTT ATCAGTCTTC 
CTTCTGCTCC CAGAGTGTCC TGTCATGCTT GATTCCAGGA ACTATATAAC TCTGGTGGTT 
CCATTTGCAG AGGCTATTCG TAAAATGACC AATCAATCTT CAAAAGTCCT GAAGCGGTGT 
TGGACGTCTT TGCAAGAATC TTCTCTCAGC TCACTTGTCC AGATGCTGAA AACTGCCATC 
ATCTCTCAAA TGGTTTGCTG GACTGAAACT GTTCAGAGTA ACCACAATGT TAAAACTCTT 
CTGGAAGTGA TGAAAGACGT GTATAAGGTA AACAAAACTA ACTGTCGATT ATCAGAAGAT 
ACGTTCCACA TAAATGAAGT CTCCTTTTTG TTGAACTTTT ATGAAGATAG AATAAGAGTG 
ATTAATAGGG AAAAAAACCT GATACCTGCA GAAAACTTCA GTCCGATTAT TTTCAGTGAT 
TTTCCATTTG TCTTTAATTT GATGTCCAAA ATTAAATTAT TGCAAGCTGA TTCCCAAATA 
AGAGTGCTGA AATCTGAAGT GAACAACTAC ATGGATTTTA GGGGAATAAT TCTGCAAAGA 
AGGGATGAAT CTCCTTTATT TACACTTAGC GTCAGACGAA GTCACTTAGT TGAAGATGCT 
CTATGTCAGT TAAGCCAAGC TGAAGACACT GACCTCCAGA AAATATTAGT GGTTGAATTT 
ATTAAAGAAA TTCGTTCTGG AGGGGAAGGG GTGAAGTCTG AGTTTTTTCA CTGTATATTT 
GAATCTATGA CCAAGGAAGA ATATGGAATG TTCATGTATC CCGAAGAGGA TTCCTACATG 
TGGTTTCCTG TCAAACCTAA ATTTGAGAAG AAGATGTATT TCCTCTTTGG GATGCTGTGT 
GGACTCTCCC TATATAATTT TAATGTTGTC TACCTCCCTT TCCCACTGGC TCTGTTTAAG 
AAACTTCTGG ACCAGGAGCC ATCATTGGAA GATTTAAAAG AACTCAGTCC TTCTTTTGGA 
AAGTGCTTGC AAGAAGTTCT AAATGATGAT GCTAATGACA TTAAAGAAGA ATTTGGCCTA 
CGTTTTTCTA TACACTGGGA CCAAAATGAT GTTGATTTAA TTCCAAATGG GATTTCTGTA 
TTTGTAGACC AAAGCAACAA GAAAGACTAT GTTTCTAAGT GTGTTGATTA TGTTTTAAAC 
ACCTCTGTAA AGGCAGTTTA TGAGGAATTT CAGAGAGGAT TTTATAAACT TTTTGACAAA 
GAGATACTTA AACATTTCAA GCCTGAAGAA CTAATGAGAG CAATAATTGG AAATACTGAC 
TATGACTGGG AACAATTTGA AAAGAATTCT ATTTATGAGC AAGGATACCA TGAGTCACAT 
CCTACTATAC TGATGTTTTG GAAAGCTTTC CATAGTTTAA CTTTGGATGA AAAGAGAAAA 
TTCCTCTTTT TTCTTACAGG AAATGATAGA CTACATGTAA AAGGCATACA GAAACCAGGA 
ATCCGGTTTC GCTGTCCTGA GACTTGCAGT GAAAGCGATT TCCCAAAATC ACTGACATGT 
CATAATATTC TGGAACTCCC TGAATATTCT ACAATGAAAA AAGTGAAGGA AGCACTTCAA 
AGAGCCATCA ACAGCAACAA AGGATTTATC TCAGCCACAG TCACAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC6 ( NM_001192644.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179573.3 Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), mRNA.

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CloneID	OBa01694
Clone ID Related Accession (Same CDS sequence)	NM_001192644.1 , NM_001192644.3
Accession Version	NM_001192644.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase HERC6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000006.1. On May 30, 2018 this sequence version replaced NM_001192644.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGGATA CTAGTTCCAC CAGTGTTCCC AGGAAAACCC TGCCAGAAAT AAGCCGAATT 
AACCAGTCTC TGACAGAAAA ATGGATGGCA GTGGCAAAAA GAAGTATAGA AGATGAAGTG 
GCTAAGAGTG AAATTAGAGT GATATTTTCA TCTCCTGCTT GTCTGACTGC AAGTTTTTTA 
AAGAAGAGAG AACCTGGAGA AATGATTTCC ATTGATGTGG ACTTAGAAAT GGCAAGAGAT 
ACCTTCAAGA AGTTAACAGA AAAAGAATGG ATTTCTTCGA TGATAACCGC ATGTCTCAAG 
GATAATCTGC TTGGGGCTCT TCCATGTCAT TCTTCACACC AAGAAGCTTT ATCAGTCTTC 
CTTCTGCTCC CAGAGTGTCC TGTCATGCTT GATTCCAGGA ACTATATAAC TCTGGTGGTT 
CCATTTGCAG AGGCTATTCG TAAAATGACC AATCAATCTT CAAAAGTCCT GAAGCGGTGT 
TGGACGTCTT TGCAAGAATC TTCTCTCAGC TCACTTGTCC AGATGCTGAA AACTGCCATC 
ATCTCTCAAA TGGTTTGCTG GACTGAAACT GTTCAGAGTA ACCACAATGT TAAAACTCTT 
CTGGAAGTGA TGAAAGACGT GTATAAGGTA AACAAAACTA ACTGTCGATT ATCAGAAGAT 
ACGTTCCACA TAAATGAAGT CTCCTTTTTG TTGAACTTTT ATGAAGATAG AATAAGAGTG 
ATTAATAGGG AAAAAAACCT GATACCTGCA GAAAACTTCA GTCCGATTAT TTTCAGTGAT 
TTTCCATTTG TCTTTAATTT GATGTCCAAA ATTAAATTAT TGCAAGCTGA TTCCCAAATA 
AGAGTGCTGA AATCTGAAGT GAACAACTAC ATGGATTTTA GGGGAATAAT TCTGCAAAGA 
AGGGATGAAT CTCCTTTATT TACACTTAGC GTCAGACGAA GTCACTTAGT TGAAGATGCT 
CTATGTCAGT TAAGCCAAGC TGAAGACACT GACCTCCAGA AAATATTAGT GGTTGAATTT 
ATTAAAGAAA TTCGTTCTGG AGGGGAAGGG GTGAAGTCTG AGTTTTTTCA CTGTATATTT 
GAATCTATGA CCAAGGAAGA ATATGGAATG TTCATGTATC CCGAAGAGGA TTCCTACATG 
TGGTTTCCTG TCAAACCTAA ATTTGAGAAG AAGATGTATT TCCTCTTTGG GATGCTGTGT 
GGACTCTCCC TATATAATTT TAATGTTGTC TACCTCCCTT TCCCACTGGC TCTGTTTAAG 
AAACTTCTGG ACCAGGAGCC ATCATTGGAA GATTTAAAAG AACTCAGTCC TTCTTTTGGA 
AAGTGCTTGC AAGAAGTTCT AAATGATGAT GCTAATGACA TTAAAGAAGA ATTTGGCCTA 
CGTTTTTCTA TACACTGGGA CCAAAATGAT GTTGATTTAA TTCCAAATGG GATTTCTGTA 
TTTGTAGACC AAAGCAACAA GAAAGACTAT GTTTCTAAGT GTGTTGATTA TGTTTTAAAC 
ACCTCTGTAA AGGCAGTTTA TGAGGAATTT CAGAGAGGAT TTTATAAACT TTTTGACAAA 
GAGATACTTA AACATTTCAA GCCTGAAGAA CTAATGAGAG CAATAATTGG AAATACTGAC 
TATGACTGGG AACAATTTGA AAAGAATTCT ATTTATGAGC AAGGATACCA TGAGTCACAT 
CCTACTATAC TGATGTTTTG GAAAGCTTTC CATAGTTTAA CTTTGGATGA AAAGAGAAAA 
TTCCTCTTTT TTCTTACAGG AAATGATAGA CTACATGTAA AAGGCATACA GAAACCAGGA 
ATCCGGTTTC GCTGTCCTGA GACTTGCAGT GAAAGCGATT TCCCAAAATC ACTGACATGT 
CATAATATTC TGGAACTCCC TGAATATTCT ACAATGAAAA AAGTGAAGGA AGCACTTCAA 
AGAGCCATCA ACAGCAACAA AGGATTTATC TCAGCCACAG TCACAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179573.3
CDS	121..3171
Translation	MYFCWRAGGRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLNVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGEFKETTFIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGTNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGHHSFALSLSGTSFGWGSNSAGQLALSGNNAPVQRYKPVSIGALKTLGVVSISCGYKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSDHTLAYIYTTGQVVSFGRGPGCASRSPHPGAESSDISCLVSAKDLADVQVKHIFAGTYANFVTTSQDTSSTSVPRKTLPEISRINQSLTEKWMAVAKRSIEDEVAKSEIRVIFSSPACLTASFLKKREPGEMISIDVDLEMARDTFKKLTEKEWISSMITACLKDNLLGALPCHSSHQEALSVFLLLPECPVMLDSRNYITLVVPFAEAIRKMTNQSSKVLKRCWTSLQESSLSSLVQMLKTAIISQMVCWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLSEDTFHINEVSFLLNFYEDRIRVINREKNLIPAENFSPIIFSDFPFVFNLMSKIKLLQADSQIRVLKSEVNNYMDFRGIILQRRDESPLFTLSVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSGGEGVKSEFFHCIFESMTKEEYGMFMYPEEDSYMWFPVKPKFEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLDQEPSLEDLKELSPSFGKCLQEVLNDDANDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVLNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHSLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFRCPETCSESDFPKSLTCHNILELPEYSTMKKVKEALQRAINSNKGFISATVTG

Target ORF information:

RefSeq Version	NM_001192644.3
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192644.3

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGGATA CTAGTTCCAC CAGTGTTCCC AGGAAAACCC TGCCAGAAAT AAGCCGAATT 
AACCAGTCTC TGACAGAAAA ATGGATGGCA GTGGCAAAAA GAAGTATAGA AGATGAAGTG 
GCTAAGAGTG AAATTAGAGT GATATTTTCA TCTCCTGCTT GTCTGACTGC AAGTTTTTTA 
AAGAAGAGAG AACCTGGAGA AATGATTTCC ATTGATGTGG ACTTAGAAAT GGCAAGAGAT 
ACCTTCAAGA AGTTAACAGA AAAAGAATGG ATTTCTTCGA TGATAACCGC ATGTCTCAAG 
GATAATCTGC TTGGGGCTCT TCCATGTCAT TCTTCACACC AAGAAGCTTT ATCAGTCTTC 
CTTCTGCTCC CAGAGTGTCC TGTCATGCTT GATTCCAGGA ACTATATAAC TCTGGTGGTT 
CCATTTGCAG AGGCTATTCG TAAAATGACC AATCAATCTT CAAAAGTCCT GAAGCGGTGT 
TGGACGTCTT TGCAAGAATC TTCTCTCAGC TCACTTGTCC AGATGCTGAA AACTGCCATC 
ATCTCTCAAA TGGTTTGCTG GACTGAAACT GTTCAGAGTA ACCACAATGT TAAAACTCTT 
CTGGAAGTGA TGAAAGACGT GTATAAGGTA AACAAAACTA ACTGTCGATT ATCAGAAGAT 
ACGTTCCACA TAAATGAAGT CTCCTTTTTG TTGAACTTTT ATGAAGATAG AATAAGAGTG 
ATTAATAGGG AAAAAAACCT GATACCTGCA GAAAACTTCA GTCCGATTAT TTTCAGTGAT 
TTTCCATTTG TCTTTAATTT GATGTCCAAA ATTAAATTAT TGCAAGCTGA TTCCCAAATA 
AGAGTGCTGA AATCTGAAGT GAACAACTAC ATGGATTTTA GGGGAATAAT TCTGCAAAGA 
AGGGATGAAT CTCCTTTATT TACACTTAGC GTCAGACGAA GTCACTTAGT TGAAGATGCT 
CTATGTCAGT TAAGCCAAGC TGAAGACACT GACCTCCAGA AAATATTAGT GGTTGAATTT 
ATTAAAGAAA TTCGTTCTGG AGGGGAAGGG GTGAAGTCTG AGTTTTTTCA CTGTATATTT 
GAATCTATGA CCAAGGAAGA ATATGGAATG TTCATGTATC CCGAAGAGGA TTCCTACATG 
TGGTTTCCTG TCAAACCTAA ATTTGAGAAG AAGATGTATT TCCTCTTTGG GATGCTGTGT 
GGACTCTCCC TATATAATTT TAATGTTGTC TACCTCCCTT TCCCACTGGC TCTGTTTAAG 
AAACTTCTGG ACCAGGAGCC ATCATTGGAA GATTTAAAAG AACTCAGTCC TTCTTTTGGA 
AAGTGCTTGC AAGAAGTTCT AAATGATGAT GCTAATGACA TTAAAGAAGA ATTTGGCCTA 
CGTTTTTCTA TACACTGGGA CCAAAATGAT GTTGATTTAA TTCCAAATGG GATTTCTGTA 
TTTGTAGACC AAAGCAACAA GAAAGACTAT GTTTCTAAGT GTGTTGATTA TGTTTTAAAC 
ACCTCTGTAA AGGCAGTTTA TGAGGAATTT CAGAGAGGAT TTTATAAACT TTTTGACAAA 
GAGATACTTA AACATTTCAA GCCTGAAGAA CTAATGAGAG CAATAATTGG AAATACTGAC 
TATGACTGGG AACAATTTGA AAAGAATTCT ATTTATGAGC AAGGATACCA TGAGTCACAT 
CCTACTATAC TGATGTTTTG GAAAGCTTTC CATAGTTTAA CTTTGGATGA AAAGAGAAAA 
TTCCTCTTTT TTCTTACAGG AAATGATAGA CTACATGTAA AAGGCATACA GAAACCAGGA 
ATCCGGTTTC GCTGTCCTGA GACTTGCAGT GAAAGCGATT TCCCAAAATC ACTGACATGT 
CATAATATTC TGGAACTCCC TGAATATTCT ACAATGAAAA AAGTGAAGGA AGCACTTCAA 
AGAGCCATCA ACAGCAACAA AGGATTTATC TCAGCCACAG TCACAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC6 ( XM_010806029.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804331.1 Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X1, mRNA.

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CloneID	OBa51406
Clone ID Related Accession (Same CDS sequence)	XM_010806029.2
Accession Version	XM_010806029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103970.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGAAGG ATACTAGTTC CACCAGTGTT CCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGACAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATGATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
GATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CGCATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CATTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTATAT AACTCTGGTG 
GTTCCATTTG CAGAGGCTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCGG 
TGTTGGACGT CTTTGCAAGA ATCTTCTCTC AGCTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATCTCTC AAATGGTTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG GTAAACAAAA CTAACTGTCG ATTATCAGAA 
GATACGTTCC ACATAAATGA AGTCTCCTTT TTGTTGAACT TTTATGAAGA TAGAATAAGA 
GTGATTAATA GGGAAAAAAA CCTGATACCT GCAGAAAACT TCAGTCCGAT TATTTTCAGT 
GATTTTCCAT TTGTCTTTAA TTTGATGTCC AAAATTAAAT TATTGCAAGC TGATTCCCAA 
ATAAGAGTGC TGAAATCTGA AGTGAACAAC TACATGGATT TTAGGGGAAT AATTCTGCAA 
AGAAGGGATG AATCTCCTTT ATTTACACTT AGCGTCAGAC GAAGTCACTT AGTTGAAGAT 
GCTCTATGTC AGTTAAGCCA AGCTGAAGAC ACTGACCTCC AGAAAATATT AGTGGTTGAA 
TTTATTAAAG AAATTCGTTC TGGAGGGGAA GGGGTGAAGT CTGAGTTTTT TCACTGTATA 
TTTGAATCTA TGACCAAGGA AGAATATGGA ATGTTCATGT ATCCCGAAGA GGATTCCTAC 
ATGTGGTTTC CTGTCAAACC TAAATTTGAG AAGAAGATGT ATTTCCTCTT TGGGATGCTG 
TGTGGACTCT CCCTATATAA TTTTAATGTT GTCTACCTCC CTTTCCCACT GGCTCTGTTT 
AAGAAACTTC TGGACCAGGA GCCATCATTG GAAGATTTAA AAGAACTCAG TCCTTCTTTT 
GGAAAGTGCT TGCAAGAAGT TCTAAATGAT GATGTTAATG ACATTAAAGA AGAATTTGGC 
CTACGTTTTT CTATACACTG GGACCAAAAT GATGTTGATT TAATTCCAAA TGGGATTTCT 
GTATTTGTAG ACCAAAGCAA CAAGAAAGAC TATGTTTCTA AGTGTGTTGA TTATGTTTTA 
AACACCTCTG TAAAGGCAGT TTATGAGGAA TTTCAGAGAG GATTTTATAA ACTTTTTGAC 
AAAGAGATAC TTAAACATTT CAAGCCTGAA GAACTAATGA GAGCAATAAT TGGAAATACT 
GACTATGACT GGGAACAATT TGAAAAGAAT TCTATTTATG AGCAAGGATA CCATGAGTCA 
CATCCTACTA TACTGATGTT TTGGAAAGCT TTCCATAGTT TAACTTTGGA TGAAAAGAGA 
AAATTCCTCT TTTTTCTTAC AGGAAATGAT AGACTACATG TAAAAGGCAT ACAGAAACCA 
GGAATCCGGT TTCGCTGTCC TGAGACTTGC AGTGAAAGCG ATTTCCCAAA ATCACTGACA 
TGTCATAATA TTCTGGAACT CCCTGAATAT TCTACAATGA AAAAAGTGAA GGAAGCACTT 
CAAAGAGCCA TCAACAGCAA CAAAGGATTT ATCTCAGCCA CAGTCACAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804331.1
CDS	137..3190
Translation	MYFCWRAGGRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLNVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGEFKETTFIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGTNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGHHSFALSLSGTSFGWGSNSAGQLALSGNNAPVQRYKPVSIGALKTLGVVSISCGYKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSDHTLAYIYTTGQVVSFGRGPGCASRSPHPGAESSDISCLVSAKDLADVQVKHIFAGTYANFVTTSQKDTSSTSVPRKTLPEISRINQSLTEKWMAVAKRSIEDEVAKSEIRVIFSSPACLTASFLKKREPGEMISIDVDLEMARDTFKKLTEKEWISSMITACLKDNLLGALPCHSSHQEALSVFLLLPECPVMLDSRNYITLVVPFAEAIRKMTNQSSKVLKRCWTSLQESSLSSLVQMLKTAIISQMVCWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLSEDTFHINEVSFLLNFYEDRIRVINREKNLIPAENFSPIIFSDFPFVFNLMSKIKLLQADSQIRVLKSEVNNYMDFRGIILQRRDESPLFTLSVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSGGEGVKSEFFHCIFESMTKEEYGMFMYPEEDSYMWFPVKPKFEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLDQEPSLEDLKELSPSFGKCLQEVLNDDVNDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVLNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHSLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFRCPETCSESDFPKSLTCHNILELPEYSTMKKVKEALQRAINSNKGFISATVTG

Target ORF information:

RefSeq Version	XM_010806029.2
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806029.2

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCGGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGAGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGAACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT CGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GAATTTAAGG AAACAACTTT TATACCTAAG 
AAGATAAAAA CTCTGGCTGG TATCAAAATT ATACAAGTTG CCTGTGGACA CTACCACTCC 
GTGGCATTAT CAGAAGATGG CCAAGTGTTT TCATGGGGGA CGAACAGCCA TGGACAGCTG 
GGCCTGGGGA AGGAATTCCC CTCCCAAGCC AGCCCACAGA GGGTGAGGTC CCTGGAGGGG 
ATCCCGCTGG CACAGGTGGC TGCAGGAGGG CACCACAGCT TTGCCCTGTC ACTCTCTGGG 
ACTTCATTTG GCTGGGGAAG CAACAGTGCA GGGCAGCTGG CCCTCAGTGG GAATAACGCC 
CCAGTTCAAA GATATAAGCC TGTATCAATT GGTGCACTGA AGACTCTCGG TGTGGTTTCT 
ATCAGCTGTG GTTACAAGCA CACTGCAGTG CTTACCCAGG ATGGGAAAGT ATTCACATTT 
GGAGACAATA GCTATGGACA GCTGGGACAT GATCCCACTG CTGAGAAGAG GGGTCCACAA 
CTTGTGGAAA GAATTGAAGG CCTCGTCTCA CAGATAGACT GTGGAAGCGA TCACACCCTT 
GCATACATCT ATACCACGGG TCAGGTGGTG TCTTTTGGTC GTGGACCAGG TTGTGCAAGC 
AGATCACCAC ATCCGGGGGC AGAGAGCTCT GACATTAGCT GCCTGGTTTC TGCTAAGGAC 
CTTGCGGATG TTCAAGTCAA ACATATTTTT GCTGGAACAT ATGCCAACTT TGTGACAACT 
TCCCAGAAGG ATACTAGTTC CACCAGTGTT CCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGACAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATGATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
GATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CGCATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CATTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTATAT AACTCTGGTG 
GTTCCATTTG CAGAGGCTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCGG 
TGTTGGACGT CTTTGCAAGA ATCTTCTCTC AGCTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATCTCTC AAATGGTTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG GTAAACAAAA CTAACTGTCG ATTATCAGAA 
GATACGTTCC ACATAAATGA AGTCTCCTTT TTGTTGAACT TTTATGAAGA TAGAATAAGA 
GTGATTAATA GGGAAAAAAA CCTGATACCT GCAGAAAACT TCAGTCCGAT TATTTTCAGT 
GATTTTCCAT TTGTCTTTAA TTTGATGTCC AAAATTAAAT TATTGCAAGC TGATTCCCAA 
ATAAGAGTGC TGAAATCTGA AGTGAACAAC TACATGGATT TTAGGGGAAT AATTCTGCAA 
AGAAGGGATG AATCTCCTTT ATTTACACTT AGCGTCAGAC GAAGTCACTT AGTTGAAGAT 
GCTCTATGTC AGTTAAGCCA AGCTGAAGAC ACTGACCTCC AGAAAATATT AGTGGTTGAA 
TTTATTAAAG AAATTCGTTC TGGAGGGGAA GGGGTGAAGT CTGAGTTTTT TCACTGTATA 
TTTGAATCTA TGACCAAGGA AGAATATGGA ATGTTCATGT ATCCCGAAGA GGATTCCTAC 
ATGTGGTTTC CTGTCAAACC TAAATTTGAG AAGAAGATGT ATTTCCTCTT TGGGATGCTG 
TGTGGACTCT CCCTATATAA TTTTAATGTT GTCTACCTCC CTTTCCCACT GGCTCTGTTT 
AAGAAACTTC TGGACCAGGA GCCATCATTG GAAGATTTAA AAGAACTCAG TCCTTCTTTT 
GGAAAGTGCT TGCAAGAAGT TCTAAATGAT GATGTTAATG ACATTAAAGA AGAATTTGGC 
CTACGTTTTT CTATACACTG GGACCAAAAT GATGTTGATT TAATTCCAAA TGGGATTTCT 
GTATTTGTAG ACCAAAGCAA CAAGAAAGAC TATGTTTCTA AGTGTGTTGA TTATGTTTTA 
AACACCTCTG TAAAGGCAGT TTATGAGGAA TTTCAGAGAG GATTTTATAA ACTTTTTGAC 
AAAGAGATAC TTAAACATTT CAAGCCTGAA GAACTAATGA GAGCAATAAT TGGAAATACT 
GACTATGACT GGGAACAATT TGAAAAGAAT TCTATTTATG AGCAAGGATA CCATGAGTCA 
CATCCTACTA TACTGATGTT TTGGAAAGCT TTCCATAGTT TAACTTTGGA TGAAAAGAGA 
AAATTCCTCT TTTTTCTTAC AGGAAATGAT AGACTACATG TAAAAGGCAT ACAGAAACCA 
GGAATCCGGT TTCGCTGTCC TGAGACTTGC AGTGAAAGCG ATTTCCCAAA ATCACTGACA 
TGTCATAATA TTCTGGAACT CCCTGAATAT TCTACAATGA AAAAAGTGAA GGAAGCACTT 
CAAAGAGCCA TCAACAGCAA CAAAGGATTT ATCTCAGCCA CAGTCACAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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HERC6 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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