Hace1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hace1
Entrez Gene ID	361866
Full Name	HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1
General protein information	Preferred Names HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase HACE1 HECT-type E3 ubiquitin transferase HACE1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q13

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256595.3	XP_006256657.1	E3 ubiquitin-protein ligase HACE1 isoform X1
NM_001108539.3	NP_001102009.2	E3 ubiquitin-protein ligase HACE1
XM_006256598.2	XP_006256660.1	E3 ubiquitin-protein ligase HACE1 isoform X4
XM_017601695.1	XP_017457184.1	E3 ubiquitin-protein ligase HACE1 isoform X5
XM_006256597.3	XP_006256659.1	E3 ubiquitin-protein ligase HACE1 isoform X2
XM_017601694.1	XP_017457183.1	E3 ubiquitin-protein ligase HACE1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	HACE1	NP_065822.2
Canis lupus familiaris (dog)	HACE1	XP_854232.1
Pan troglodytes (chimpanzee)	HACE1	XP_518654.2
Rattus norvegicus (Norway rat)	Hace1	NP_001102009.2
Gallus gallus (chicken)	HACE1	NP_001265084.1
Xenopus tropicalis (tropical clawed frog)	hace1	NP_001039269.1
Bos taurus (cattle)	HACE1	NP_001179574.2
Mus musculus (house mouse)	Hace1	NP_766061.2
Danio rerio (zebrafish)	hace1	XP_701235.5

Gene Ontology
Bibliography

Related articles in PubMed

The E3 ubiquitin-ligase HACE1 catalyzes the ubiquitylation of active Rac1.
Torrino S, Visvikis O, Doye A, Boyer L, Stefani C, Munro P, Bertoglio J, Gacon G, Mettouchi A, Lemichez E
Developmental cell21(5)959-65(2011 Nov)

The ubiquitin ligase HACE1 regulates Golgi membrane dynamics during the cell cycle.
Tang D, Xiang Y, De Renzis S, Rink J, Zheng G, Zerial M, Wang Y
Nature communications2501(2011 Oct)

Differential expression of a novel ankyrin containing E3 ubiquitin-protein ligase, Hace1, in sporadic Wilms' tumor versus normal kidney.
Anglesio MS, Evdokimova V, Melnyk N, Zhang L, Fernandez CV, Grundy PE, Leach S, Marra MA, Brooks-Wilson AR, Penninger J, Sorensen PH
Human molecular genetics13(18)2061-74(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression analysis of the human Hace1 ortholog and demonstration of its ubiquitin ligase activity.
Title: Differential expression of a novel ankyrin containing E3 ubiquitin-protein ligase, Hace1, in sporadic Wilms' tumor versus normal kidney.

The following Hace1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hace1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00363	NM_001108539.3 Latest version!	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
ORa39553	XM_006256595.3 Latest version!	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa39555	XM_006256597.3 Latest version!	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa39556	XM_006256598.2 Latest version!	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa60132	XM_017601694.1 Latest version!	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa60133	XM_017601695.1 Latest version!	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hace1 ( NM_001108539.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102009.2 Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), mRNA.

Price & Availability↑

CloneID	ORa00363
Clone ID Related Accession (Same CDS sequence)	NM_001108539.3
Accession Version	NM_001108539.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-23
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HACE1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07045530.1, AABR07045531.1, AABR07045532.1 and AABR07045534.1. On Nov 27, 2008 this sequence version replaced NM_001108539.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG 
GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC 
TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG 
CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC 
AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG 
AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG 
GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG 
CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA 
CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA 
GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC 
TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT 
CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG 
TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA 
ACAAATGGCC ATAAACTGCT TAGTCTGTCC AGCAGCTACG AGGCGCAGAT GAAGAGCCTC 
CTGCGGATCG TGAGGATCTT CTGCCACGTC TTCCGCATTG GCCCCTCGTC TCCCAGTAAC 
GGCATGGATA TGGGCTACAA TGGGAATAAG ACTCCAAGGA GCCAGGTGTT CAAGCCTTTG 
GAATTGCTTT GGCACTCTCT GGATGAGTGG TTGGTTTTAA TAGCCACAGA ATTAATGAAG 
AACAAAGAGG ACTCAGCGGA TATCACCTCC ATTTTACTGA AACAGAAAGG CCAGGATCAA 
GAGGCCTCTT GTATTTCAGC GTTTGAGCCA CCAGGACCTG GGAGCTATGA ACGCCTGTCC 
ACTGGTCCCG GGGAATCCAA ACCAGATGTG CTTGCCGGGA AGCAGGAAGC CAGTGCGGAC 
TGTCAGGATG TCATTTCTGT GACGGCCAAC CGGCTAAGTG CTGTCATTCA AGCCTTTTAC 
ATGTGCTGCT CCTGTCAGAT GCCTCCAGGA ATGACGTCGC CTCGGTTCAT TGAGTTTGTC 
TGCAAGCATG ATGAAGTGCT AAAGTGCTTT GTGAATAGAA ATCCCAAAAT TATATTTGAC 
CATTTTCACT TTCTTCTTGA ATGTCCTGAA CTGATGTCGA GATTCATGCA CATCATAAAG 
GCACAGCCAT TCAAAGACCG CTGTGAATGG TTCTATGAGC ATTTGCACTC AGGACAGCCA 
GACTCAGACA TGGTGCACAG ACCAGTGAGT GAAAACGACA TTCTCCTGGT TCACAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAATCGTA TCAAAGGCCA ACTGTGCAAA GCTGAAGCAG 
GGGATCGCTG TGCGCTTTCA TGGAGAGGAA GGCATGGGTC AAGGTGTTGT ACGTGAATGG 
TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGACTATG CACTGTTTAC CCAGTCAGCA 
GATGGAACAA CTTTTCAGCC CAATAGCAAC TCCTATGTCA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAATC ACAGGCAGTT GGTCAATATT 
TACTTCACAC GATCCTTCTA CAAACACATT CTTGGTATTC CTGTGAATTA CCAAGATGTA 
GCGTCTATCG ATCCAGAATA TGCCAAAAAT CTGCAGTGGA TTTTAGACAA TGACATTAGT 
GACCTGGGTC TAGAACTGAC TTTCTCTGTT GAGACTGATG TGTTTGGAGC TATGGAAGAG 
GTACCGTTAA AGCCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAC 
GTCCAGCTGG TTACTGAACT CCGGATGACA AGAGCCATTC AGCCTCAGAT CAATGCGTTT 
TTACAGGGTT TCCATATGTT CATCCCTCCT TCCCTCATAC AGCTGTTCGA CGAGTATGAA 
TTGGAGCTAC TGCTCTCTGG CATGCCGGAA ATTGACGTGA ATGATTGGAT CAAAAATACA 
GAATACACAA GTGGCTATGA AAGGGAAGAT CCAGTTATTC AGTGGTTCTG GGAGGTTGTG 
GAGGACATGA CTCAAGAAGA GCGGGTCCTT CTCTTACAGT TCGTGACTGG CAGTTCCAGG 
GTCCCGCATG GTGGCTTTGC CAATATCATG GGTGGCAGTG GTTTGCAAAA CTTTACAATT 
GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCCA GCACGTGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG TAAAGAGATC CTCAAGGACA GACTCCTCGT AGCTCTGCAT 
TGTGGCAGCT ACGGCTATAC AATGGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102009.2
CDS	1..2730
Translation	MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVAGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHLSQQSESQYLKILTGLAEVATTNGHKLLSLSSSYEAQMKSLLRIVRIFCHVFRIGPSSPSNGMDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKEDSADITSILLKQKGQDQEASCISAFEPPGPGSYERLSTGPGESKPDVLAGKQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVSENDILLVHRDSIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDMTQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001108539.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108539.3

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG 
GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC 
TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG 
CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC 
AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG 
AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG 
GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG 
CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA 
CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA 
GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC 
TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT 
CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG 
TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA 
ACAAATGGCC ATAAACTGCT TAGTCTGTCC AGCAGCTACG AGGCGCAGAT GAAGAGCCTC 
CTGCGGATCG TGAGGATCTT CTGCCACGTC TTCCGCATTG GCCCCTCGTC TCCCAGTAAC 
GGCATGGATA TGGGCTACAA TGGGAATAAG ACTCCAAGGA GCCAGGTGTT CAAGCCTTTG 
GAATTGCTTT GGCACTCTCT GGATGAGTGG TTGGTTTTAA TAGCCACAGA ATTAATGAAG 
AACAAAGAGG ACTCAGCGGA TATCACCTCC ATTTTACTGA AACAGAAAGG CCAGGATCAA 
GAGGCCTCTT GTATTTCAGC GTTTGAGCCA CCAGGACCTG GGAGCTATGA ACGCCTGTCC 
ACTGGTCCCG GGGAATCCAA ACCAGATGTG CTTGCCGGGA AGCAGGAAGC CAGTGCGGAC 
TGTCAGGATG TCATTTCTGT GACGGCCAAC CGGCTAAGTG CTGTCATTCA AGCCTTTTAC 
ATGTGCTGCT CCTGTCAGAT GCCTCCAGGA ATGACGTCGC CTCGGTTCAT TGAGTTTGTC 
TGCAAGCATG ATGAAGTGCT AAAGTGCTTT GTGAATAGAA ATCCCAAAAT TATATTTGAC 
CATTTTCACT TTCTTCTTGA ATGTCCTGAA CTGATGTCGA GATTCATGCA CATCATAAAG 
GCACAGCCAT TCAAAGACCG CTGTGAATGG TTCTATGAGC ATTTGCACTC AGGACAGCCA 
GACTCAGACA TGGTGCACAG ACCAGTGAGT GAAAACGACA TTCTCCTGGT TCACAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAATCGTA TCAAAGGCCA ACTGTGCAAA GCTGAAGCAG 
GGGATCGCTG TGCGCTTTCA TGGAGAGGAA GGCATGGGTC AAGGTGTTGT ACGTGAATGG 
TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGACTATG CACTGTTTAC CCAGTCAGCA 
GATGGAACAA CTTTTCAGCC CAATAGCAAC TCCTATGTCA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAATC ACAGGCAGTT GGTCAATATT 
TACTTCACAC GATCCTTCTA CAAACACATT CTTGGTATTC CTGTGAATTA CCAAGATGTA 
GCGTCTATCG ATCCAGAATA TGCCAAAAAT CTGCAGTGGA TTTTAGACAA TGACATTAGT 
GACCTGGGTC TAGAACTGAC TTTCTCTGTT GAGACTGATG TGTTTGGAGC TATGGAAGAG 
GTACCGTTAA AGCCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAC 
GTCCAGCTGG TTACTGAACT CCGGATGACA AGAGCCATTC AGCCTCAGAT CAATGCGTTT 
TTACAGGGTT TCCATATGTT CATCCCTCCT TCCCTCATAC AGCTGTTCGA CGAGTATGAA 
TTGGAGCTAC TGCTCTCTGG CATGCCGGAA ATTGACGTGA ATGATTGGAT CAAAAATACA 
GAATACACAA GTGGCTATGA AAGGGAAGAT CCAGTTATTC AGTGGTTCTG GGAGGTTGTG 
GAGGACATGA CTCAAGAAGA GCGGGTCCTT CTCTTACAGT TCGTGACTGG CAGTTCCAGG 
GTCCCGCATG GTGGCTTTGC CAATATCATG GGTGGCAGTG GTTTGCAAAA CTTTACAATT 
GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCCA GCACGTGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG TAAAGAGATC CTCAAGGACA GACTCCTCGT AGCTCTGCAT 
TGTGGCAGCT ACGGCTATAC AATGGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hace1 ( XM_006256595.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256657.1 Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39553
Clone ID Related Accession (Same CDS sequence)	XM_006256595.3
Accession Version	XM_006256595.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HACE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256595.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG 
GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC 
TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG 
CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC 
AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG 
AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG 
GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG 
CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA 
CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA 
GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC 
TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT 
CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG 
TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA 
ACAAATGGCC ATAAACTGCT TAGTGTGTTC CGGTTGGTCT CCAGTCTGTC CAGCAGCTAC 
GAGGCGCAGA TGAAGAGCCT CCTGCGGATC GTGAGGATCT TCTGCCACGT CTTCCGCATT 
GGCCCCTCGT CTCCCAGTAA CGGCATGGAT ATGGGCTACA ATGGGAATAA GACTCCAAGG 
AGCCAGGTGT TCAAGCCTTT GGAATTGCTT TGGCACTCTC TGGATGAGTG GTTGGTTTTA 
ATAGCCACAG AATTAATGAA GAACAAAGAG GACTCAGCGG ATATCACCTC CATTTTACTG 
AAACAGAAAG GCCAGGATCA AGAGGCCTCT TGTATTTCAG CGTTTGAGCC ACCAGGACCT 
GGGAGCTATG AACGCCTGTC CACTGGTCCC GGGGAATCCA AACCAGATGT GCTTGCCGGG 
AAGCAGGAAG CCAGTGCGGA CTGTCAGGAT GTCATTTCTG TGACGGCCAA CCGGCTAAGT 
GCTGTCATTC AAGCCTTTTA CATGTGCTGC TCCTGTCAGA TGCCTCCAGG AATGACGTCG 
CCTCGGTTCA TTGAGTTTGT CTGCAAGCAT GATGAAGTGC TAAAGTGCTT TGTGAATAGA 
AATCCCAAAA TTATATTTGA CCATTTTCAC TTTCTTCTTG AATGTCCTGA ACTGATGTCG 
AGATTCATGC ACATCATAAA GGCACAGCCA TTCAAAGACC GCTGTGAATG GTTCTATGAG 
CATTTGCACT CAGGACAGCC AGACTCAGAC ATGGTGCACA GACCAGTGAG TGAAAACGAC 
ATTCTCCTGG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAATCGT ATCAAAGGCC 
AACTGTGCAA AGCTGAAGCA GGGGATCGCT GTGCGCTTTC ATGGAGAGGA AGGCATGGGT 
CAAGGTGTTG TACGTGAATG GTTTGATATT CTGTCTAATG AGATAGTCAA TCCTGACTAT 
GCACTGTTTA CCCAGTCAGC AGATGGAACA ACTTTTCAGC CCAATAGCAA CTCCTATGTC 
AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAT 
CACAGGCAGT TGGTCAATAT TTACTTCACA CGATCCTTCT ACAAACACAT TCTTGGTATT 
CCTGTGAATT ACCAAGATGT AGCGTCTATC GATCCAGAAT ATGCCAAAAA TCTGCAGTGG 
ATTTTAGACA ATGACATTAG TGACCTGGGT CTAGAACTGA CTTTCTCTGT TGAGACTGAT 
GTGTTTGGAG CTATGGAAGA GGTACCGTTA AAGCCTGGGG GTGGAAGTAT TCTTGTGACA 
CAAAATAACA AAGCGGAGTA CGTCCAGCTG GTTACTGAAC TCCGGATGAC AAGAGCCATT 
CAGCCTCAGA TCAATGCGTT TTTACAGGGT TTCCATATGT TCATCCCTCC TTCCCTCATA 
CAGCTGTTCG ACGAGTATGA ATTGGAGCTA CTGCTCTCTG GCATGCCGGA AATTGACGTG 
AATGATTGGA TCAAAAATAC AGAATACACA AGTGGCTATG AAAGGGAAGA TCCAGTTATT 
CAGTGGTTCT GGGAGGTTGT GGAGGACATG ACTCAAGAAG AGCGGGTCCT TCTCTTACAG 
TTCGTGACTG GCAGTTCCAG GGTCCCGCAT GGTGGCTTTG CCAATATCAT GGGTGGCAGT 
GGTTTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTTTT ACCAACTTCC 
AGCACGTGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAGAT CCTCAAGGAC 
AGACTCCTCG TAGCTCTGCA TTGTGGCAGC TACGGCTATA CAATGGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256657.1
CDS	257..3007
Translation	MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVAGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHLSQQSESQYLKILTGLAEVATTNGHKLLSVFRLVSSLSSSYEAQMKSLLRIVRIFCHVFRIGPSSPSNGMDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKEDSADITSILLKQKGQDQEASCISAFEPPGPGSYERLSTGPGESKPDVLAGKQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVSENDILLVHRDSIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDMTQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_006256595.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256595.3

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG 
GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC 
TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG 
CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC 
AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG 
AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG 
GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG 
CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA 
CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA 
GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC 
TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT 
CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG 
TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA 
ACAAATGGCC ATAAACTGCT TAGTGTGTTC CGGTTGGTCT CCAGTCTGTC CAGCAGCTAC 
GAGGCGCAGA TGAAGAGCCT CCTGCGGATC GTGAGGATCT TCTGCCACGT CTTCCGCATT 
GGCCCCTCGT CTCCCAGTAA CGGCATGGAT ATGGGCTACA ATGGGAATAA GACTCCAAGG 
AGCCAGGTGT TCAAGCCTTT GGAATTGCTT TGGCACTCTC TGGATGAGTG GTTGGTTTTA 
ATAGCCACAG AATTAATGAA GAACAAAGAG GACTCAGCGG ATATCACCTC CATTTTACTG 
AAACAGAAAG GCCAGGATCA AGAGGCCTCT TGTATTTCAG CGTTTGAGCC ACCAGGACCT 
GGGAGCTATG AACGCCTGTC CACTGGTCCC GGGGAATCCA AACCAGATGT GCTTGCCGGG 
AAGCAGGAAG CCAGTGCGGA CTGTCAGGAT GTCATTTCTG TGACGGCCAA CCGGCTAAGT 
GCTGTCATTC AAGCCTTTTA CATGTGCTGC TCCTGTCAGA TGCCTCCAGG AATGACGTCG 
CCTCGGTTCA TTGAGTTTGT CTGCAAGCAT GATGAAGTGC TAAAGTGCTT TGTGAATAGA 
AATCCCAAAA TTATATTTGA CCATTTTCAC TTTCTTCTTG AATGTCCTGA ACTGATGTCG 
AGATTCATGC ACATCATAAA GGCACAGCCA TTCAAAGACC GCTGTGAATG GTTCTATGAG 
CATTTGCACT CAGGACAGCC AGACTCAGAC ATGGTGCACA GACCAGTGAG TGAAAACGAC 
ATTCTCCTGG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAATCGT ATCAAAGGCC 
AACTGTGCAA AGCTGAAGCA GGGGATCGCT GTGCGCTTTC ATGGAGAGGA AGGCATGGGT 
CAAGGTGTTG TACGTGAATG GTTTGATATT CTGTCTAATG AGATAGTCAA TCCTGACTAT 
GCACTGTTTA CCCAGTCAGC AGATGGAACA ACTTTTCAGC CCAATAGCAA CTCCTATGTC 
AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAT 
CACAGGCAGT TGGTCAATAT TTACTTCACA CGATCCTTCT ACAAACACAT TCTTGGTATT 
CCTGTGAATT ACCAAGATGT AGCGTCTATC GATCCAGAAT ATGCCAAAAA TCTGCAGTGG 
ATTTTAGACA ATGACATTAG TGACCTGGGT CTAGAACTGA CTTTCTCTGT TGAGACTGAT 
GTGTTTGGAG CTATGGAAGA GGTACCGTTA AAGCCTGGGG GTGGAAGTAT TCTTGTGACA 
CAAAATAACA AAGCGGAGTA CGTCCAGCTG GTTACTGAAC TCCGGATGAC AAGAGCCATT 
CAGCCTCAGA TCAATGCGTT TTTACAGGGT TTCCATATGT TCATCCCTCC TTCCCTCATA 
CAGCTGTTCG ACGAGTATGA ATTGGAGCTA CTGCTCTCTG GCATGCCGGA AATTGACGTG 
AATGATTGGA TCAAAAATAC AGAATACACA AGTGGCTATG AAAGGGAAGA TCCAGTTATT 
CAGTGGTTCT GGGAGGTTGT GGAGGACATG ACTCAAGAAG AGCGGGTCCT TCTCTTACAG 
TTCGTGACTG GCAGTTCCAG GGTCCCGCAT GGTGGCTTTG CCAATATCAT GGGTGGCAGT 
GGTTTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTTTT ACCAACTTCC 
AGCACGTGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAGAT CCTCAAGGAC 
AGACTCCTCG TAGCTCTGCA TTGTGGCAGC TACGGCTATA CAATGGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hace1 ( XM_006256597.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256659.1 Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa39555
Clone ID Related Accession (Same CDS sequence)	XM_006256597.3
Accession Version	XM_006256597.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HACE1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG 
GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC 
TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG 
CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC 
AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG 
AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG 
GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG 
CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA 
CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA 
GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC 
TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT 
CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG 
TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA 
ACAAATGGCC ATAAACTGCT TAGTGTGTTC CGGTTGGTCT CCAGTCTGTC CAGCAGCTAC 
GAGGCGCAGA TGAAGAGCCT CCTGCGGATC GTGAGGATCT TCTGCCACGT CTTCCGCATT 
GGCCCCTCGT CTCCCAGTAA CGGCATGGAT ATGGGCTACA ATGGGAATAA GACTCCAAGG 
AGCCAGGTGT TCAAGCCTTT GGAATTGCTT TGGCACTCTC TGGATGAGTG GTTGGTTTTA 
ATAGCCACAG AATTAATGAA GAACAAAGAG GACTCAGCGG ATATCACCTC CATTTTACTG 
AAACAGAAAG GCCAGGATCA AGAGGCCTCT TGTATTTCAG CGTTTGAGCC ACCAGGACCT 
GGGAGCTATG AACGCCTGTC CACTGGTCCC GGGGAATCCA AACCAGATGT GCTTGCCGGG 
AAGCAGGAAG CCAGTGCGGA CTGTCAGGAT GTCATTTCTG TGACGGCCAA CCGGCTAAGT 
GCTGTCATTC AAGCCTTTTA CATGTGCTGC TCCTGTCAGA TGCCTCCAGG AATGACGTCG 
CCTCGGTTCA TTGAGTTTGT CTGCAAGCAT GATGAAGTGC TAAAGTGCTT TGTGAATAGA 
AATCCCAAAA TTATATTTGA CCATTTTCAC TTTCTTCTTG AATGTCCTGA ACTGATGTCG 
AGATTCATGC ACATCATAAA GGCACAGCCA TTCAAAGACC GCTGTGAATG GTTCTATGAG 
CATTTGCACT CAGGACAGCC AGACTCAGAC ATGGTGCACA GACCAGTGAG TGAAAACGAC 
ATTCTCCTGG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAATCGT ATCAAAGGCC 
AACTGTGCAA AGCTGAAGCA GGGGATCGCT GTGCGCTTTC ATGGAGAGGA AGGCATGGGT 
CAAGGTGTTG TACGTGAATG GTTTGATATT CTGTCTAATG AGATAGTCAA TCCTGACTAT 
GCACTGTTTA CCCAGTCAGC AGATGGAACA ACTTTTCAGC CCAATAGCAA CTCCTATGTC 
AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAT 
CACAGGCAGT TGGTCAATAT TTACTTCACA CGATCCTTCT ACAAACACAT TCTTGGTATT 
CCTGTGAATT ACCAAGATGT AGCGTCTATC GATCCAGAAT ATGCCAAAAA TCTGCAGTGG 
ATTTTAGACA ATGACATTAG TGACCTGGGT CTAGAACTGA CTTTCTCTGT TGAGACTGAT 
GTGTTTGGAG CTATGGAAGA GGTACCGTTA AAGCCTGGGG GTGGAAGTAT TCTTGTGACA 
CAAAATAACA AAGAGCTACT GCTCTCTGGC ATGCCGGAAA TTGACGTGAA TGATTGGATC 
AAAAATACAG AATACACAAG TGGCTATGAA AGGGAAGATC CAGTTATTCA GTGGTTCTGG 
GAGGTTGTGG AGGACATGAC TCAAGAAGAG CGGGTCCTTC TCTTACAGTT CGTGACTGGC 
AGTTCCAGGG TCCCGCATGG TGGCTTTGCC AATATCATGG GTGGCAGTGG TTTGCAAAAC 
TTTACAATTG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCCAG CACGTGCATC 
AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAGATCC TCAAGGACAG ACTCCTCGTA 
GCTCTGCATT GTGGCAGCTA CGGCTATACA ATGGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256659.1
CDS	257..2875
Translation	MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVAGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHLSQQSESQYLKILTGLAEVATTNGHKLLSVFRLVSSLSSSYEAQMKSLLRIVRIFCHVFRIGPSSPSNGMDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKEDSADITSILLKQKGQDQEASCISAFEPPGPGSYERLSTGPGESKPDVLAGKQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVSENDILLVHRDSIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDMTQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_006256597.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256597.3

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG 
GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC 
TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG 
CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC 
AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG 
AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG 
GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG 
CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA 
CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA 
GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC 
TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT 
CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG 
TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA 
ACAAATGGCC ATAAACTGCT TAGTGTGTTC CGGTTGGTCT CCAGTCTGTC CAGCAGCTAC 
GAGGCGCAGA TGAAGAGCCT CCTGCGGATC GTGAGGATCT TCTGCCACGT CTTCCGCATT 
GGCCCCTCGT CTCCCAGTAA CGGCATGGAT ATGGGCTACA ATGGGAATAA GACTCCAAGG 
AGCCAGGTGT TCAAGCCTTT GGAATTGCTT TGGCACTCTC TGGATGAGTG GTTGGTTTTA 
ATAGCCACAG AATTAATGAA GAACAAAGAG GACTCAGCGG ATATCACCTC CATTTTACTG 
AAACAGAAAG GCCAGGATCA AGAGGCCTCT TGTATTTCAG CGTTTGAGCC ACCAGGACCT 
GGGAGCTATG AACGCCTGTC CACTGGTCCC GGGGAATCCA AACCAGATGT GCTTGCCGGG 
AAGCAGGAAG CCAGTGCGGA CTGTCAGGAT GTCATTTCTG TGACGGCCAA CCGGCTAAGT 
GCTGTCATTC AAGCCTTTTA CATGTGCTGC TCCTGTCAGA TGCCTCCAGG AATGACGTCG 
CCTCGGTTCA TTGAGTTTGT CTGCAAGCAT GATGAAGTGC TAAAGTGCTT TGTGAATAGA 
AATCCCAAAA TTATATTTGA CCATTTTCAC TTTCTTCTTG AATGTCCTGA ACTGATGTCG 
AGATTCATGC ACATCATAAA GGCACAGCCA TTCAAAGACC GCTGTGAATG GTTCTATGAG 
CATTTGCACT CAGGACAGCC AGACTCAGAC ATGGTGCACA GACCAGTGAG TGAAAACGAC 
ATTCTCCTGG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAATCGT ATCAAAGGCC 
AACTGTGCAA AGCTGAAGCA GGGGATCGCT GTGCGCTTTC ATGGAGAGGA AGGCATGGGT 
CAAGGTGTTG TACGTGAATG GTTTGATATT CTGTCTAATG AGATAGTCAA TCCTGACTAT 
GCACTGTTTA CCCAGTCAGC AGATGGAACA ACTTTTCAGC CCAATAGCAA CTCCTATGTC 
AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAT 
CACAGGCAGT TGGTCAATAT TTACTTCACA CGATCCTTCT ACAAACACAT TCTTGGTATT 
CCTGTGAATT ACCAAGATGT AGCGTCTATC GATCCAGAAT ATGCCAAAAA TCTGCAGTGG 
ATTTTAGACA ATGACATTAG TGACCTGGGT CTAGAACTGA CTTTCTCTGT TGAGACTGAT 
GTGTTTGGAG CTATGGAAGA GGTACCGTTA AAGCCTGGGG GTGGAAGTAT TCTTGTGACA 
CAAAATAACA AAGAGCTACT GCTCTCTGGC ATGCCGGAAA TTGACGTGAA TGATTGGATC 
AAAAATACAG AATACACAAG TGGCTATGAA AGGGAAGATC CAGTTATTCA GTGGTTCTGG 
GAGGTTGTGG AGGACATGAC TCAAGAAGAG CGGGTCCTTC TCTTACAGTT CGTGACTGGC 
AGTTCCAGGG TCCCGCATGG TGGCTTTGCC AATATCATGG GTGGCAGTGG TTTGCAAAAC 
TTTACAATTG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCCAG CACGTGCATC 
AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAGATCC TCAAGGACAG ACTCCTCGTA 
GCTCTGCATT GTGGCAGCTA CGGCTATACA ATGGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hace1 ( XM_006256598.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256660.1 Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa39556
Clone ID Related Accession (Same CDS sequence)	XM_006256598.2
Accession Version	XM_006256598.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HACE1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAC AGAGCTACTC CATGACCTGG TGCAGCACGT CACGGATGTG GATGTGGAGG 
ATGCAATGGG GCAGACAGCA TTACATGTGG CCTGCCAGAA CGGGCACAAA ACGTCATGGC 
CAGAGAGATA CTGCGCAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAC 
GGAGTAACCC CTCTGGACCT GTGTGTGCAG GGTGGCTATG GACAGACTTG CGAAGTGTTA 
ATTCAGTATC ACCCCAGGCT TTTCCAGACG ATTGTTCAAA TGACGCAGAA TGAAGACCTT 
CGGGAGAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAGAGTGA AAGCCAGTAC 
CTAAAGATCC TGACAGGTCT TGCTGAAGTT GCTACAACAA ATGGCCATAA ACTGCTTAGT 
GTGTTCCGGT TGGTCTCCAG TCTGTCCAGC AGCTACGAGG CGCAGATGAA GAGCCTCCTG 
CGGATCGTGA GGATCTTCTG CCACGTCTTC CGCATTGGCC CCTCGTCTCC CAGTAACGGC 
ATGGATATGG GCTACAATGG GAATAAGACT CCAAGGAGCC AGGTGTTCAA GCCTTTGGAA 
TTGCTTTGGC ACTCTCTGGA TGAGTGGTTG GTTTTAATAG CCACAGAATT AATGAAGAAC 
AAAGAGGACT CAGCGGATAT CACCTCCATT TTACTGAAAC AGAAAGGCCA GGATCAAGAG 
GCCTCTTGTA TTTCAGCGTT TGAGCCACCA GGACCTGGGA GCTATGAACG CCTGTCCACT 
GGTCCCGGGG AATCCAAACC AGATGTGCTT GCCGGGAAGC AGGAAGCCAG TGCGGACTGT 
CAGGATGTCA TTTCTGTGAC GGCCAACCGG CTAAGTGCTG TCATTCAAGC CTTTTACATG 
TGCTGCTCCT GTCAGATGCC TCCAGGAATG ACGTCGCCTC GGTTCATTGA GTTTGTCTGC 
AAGCATGATG AAGTGCTAAA GTGCTTTGTG AATAGAAATC CCAAAATTAT ATTTGACCAT 
TTTCACTTTC TTCTTGAATG TCCTGAACTG ATGTCGAGAT TCATGCACAT CATAAAGGCA 
CAGCCATTCA AAGACCGCTG TGAATGGTTC TATGAGCATT TGCACTCAGG ACAGCCAGAC 
TCAGACATGG TGCACAGACC AGTGAGTGAA AACGACATTC TCCTGGTTCA CAGAGATTCT 
ATTTTTAGGA GTAGCTGTGA AATCGTATCA AAGGCCAACT GTGCAAAGCT GAAGCAGGGG 
ATCGCTGTGC GCTTTCATGG AGAGGAAGGC ATGGGTCAAG GTGTTGTACG TGAATGGTTT 
GATATTCTGT CTAATGAGAT AGTCAATCCT GACTATGCAC TGTTTACCCA GTCAGCAGAT 
GGAACAACTT TTCAGCCCAA TAGCAACTCC TATGTCAATC CTGATCACTT GAACTATTTC 
CGGTTTGCTG GACAGATCTT GGGATTAGCT TTGAATCACA GGCAGTTGGT CAATATTTAC 
TTCACACGAT CCTTCTACAA ACACATTCTT GGTATTCCTG TGAATTACCA AGATGTAGCG 
TCTATCGATC CAGAATATGC CAAAAATCTG CAGTGGATTT TAGACAATGA CATTAGTGAC 
CTGGGTCTAG AACTGACTTT CTCTGTTGAG ACTGATGTGT TTGGAGCTAT GGAAGAGGTA 
CCGTTAAAGC CTGGGGGTGG AAGTATTCTT GTGACACAAA ATAACAAAGC GGAGTACGTC 
CAGCTGGTTA CTGAACTCCG GATGACAAGA GCCATTCAGC CTCAGATCAA TGCGTTTTTA 
CAGGGTTTCC ATATGTTCAT CCCTCCTTCC CTCATACAGC TGTTCGACGA GTATGAATTG 
GAGCTACTGC TCTCTGGCAT GCCGGAAATT GACGTGAATG ATTGGATCAA AAATACAGAA 
TACACAAGTG GCTATGAAAG GGAAGATCCA GTTATTCAGT GGTTCTGGGA GGTTGTGGAG 
GACATGACTC AAGAAGAGCG GGTCCTTCTC TTACAGTTCG TGACTGGCAG TTCCAGGGTC 
CCGCATGGTG GCTTTGCCAA TATCATGGGT GGCAGTGGTT TGCAAAACTT TACAATTGCT 
GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCCAGCA CGTGCATCAA CATGCTCAAG 
TTACCTGAAT ACCCAAGTAA AGAGATCCTC AAGGACAGAC TCCTCGTAGC TCTGCATTGT 
GGCAGCTACG GCTATACAAT GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256660.1
CDS	96..2342
Translation	MEGQSYSMTWCSTSRMWMWRMQWGRQHYMWPARTGTKRHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHLSQQSESQYLKILTGLAEVATTNGHKLLSVFRLVSSLSSSYEAQMKSLLRIVRIFCHVFRIGPSSPSNGMDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKEDSADITSILLKQKGQDQEASCISAFEPPGPGSYERLSTGPGESKPDVLAGKQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVSENDILLVHRDSIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDMTQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_006256598.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256598.2

ORF Insert Sequence:

ATGGAAGGAC AGAGCTACTC CATGACCTGG TGCAGCACGT CACGGATGTG GATGTGGAGG 
ATGCAATGGG GCAGACAGCA TTACATGTGG CCTGCCAGAA CGGGCACAAA ACGTCATGGC 
CAGAGAGATA CTGCGCAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAC 
GGAGTAACCC CTCTGGACCT GTGTGTGCAG GGTGGCTATG GACAGACTTG CGAAGTGTTA 
ATTCAGTATC ACCCCAGGCT TTTCCAGACG ATTGTTCAAA TGACGCAGAA TGAAGACCTT 
CGGGAGAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAGAGTGA AAGCCAGTAC 
CTAAAGATCC TGACAGGTCT TGCTGAAGTT GCTACAACAA ATGGCCATAA ACTGCTTAGT 
GTGTTCCGGT TGGTCTCCAG TCTGTCCAGC AGCTACGAGG CGCAGATGAA GAGCCTCCTG 
CGGATCGTGA GGATCTTCTG CCACGTCTTC CGCATTGGCC CCTCGTCTCC CAGTAACGGC 
ATGGATATGG GCTACAATGG GAATAAGACT CCAAGGAGCC AGGTGTTCAA GCCTTTGGAA 
TTGCTTTGGC ACTCTCTGGA TGAGTGGTTG GTTTTAATAG CCACAGAATT AATGAAGAAC 
AAAGAGGACT CAGCGGATAT CACCTCCATT TTACTGAAAC AGAAAGGCCA GGATCAAGAG 
GCCTCTTGTA TTTCAGCGTT TGAGCCACCA GGACCTGGGA GCTATGAACG CCTGTCCACT 
GGTCCCGGGG AATCCAAACC AGATGTGCTT GCCGGGAAGC AGGAAGCCAG TGCGGACTGT 
CAGGATGTCA TTTCTGTGAC GGCCAACCGG CTAAGTGCTG TCATTCAAGC CTTTTACATG 
TGCTGCTCCT GTCAGATGCC TCCAGGAATG ACGTCGCCTC GGTTCATTGA GTTTGTCTGC 
AAGCATGATG AAGTGCTAAA GTGCTTTGTG AATAGAAATC CCAAAATTAT ATTTGACCAT 
TTTCACTTTC TTCTTGAATG TCCTGAACTG ATGTCGAGAT TCATGCACAT CATAAAGGCA 
CAGCCATTCA AAGACCGCTG TGAATGGTTC TATGAGCATT TGCACTCAGG ACAGCCAGAC 
TCAGACATGG TGCACAGACC AGTGAGTGAA AACGACATTC TCCTGGTTCA CAGAGATTCT 
ATTTTTAGGA GTAGCTGTGA AATCGTATCA AAGGCCAACT GTGCAAAGCT GAAGCAGGGG 
ATCGCTGTGC GCTTTCATGG AGAGGAAGGC ATGGGTCAAG GTGTTGTACG TGAATGGTTT 
GATATTCTGT CTAATGAGAT AGTCAATCCT GACTATGCAC TGTTTACCCA GTCAGCAGAT 
GGAACAACTT TTCAGCCCAA TAGCAACTCC TATGTCAATC CTGATCACTT GAACTATTTC 
CGGTTTGCTG GACAGATCTT GGGATTAGCT TTGAATCACA GGCAGTTGGT CAATATTTAC 
TTCACACGAT CCTTCTACAA ACACATTCTT GGTATTCCTG TGAATTACCA AGATGTAGCG 
TCTATCGATC CAGAATATGC CAAAAATCTG CAGTGGATTT TAGACAATGA CATTAGTGAC 
CTGGGTCTAG AACTGACTTT CTCTGTTGAG ACTGATGTGT TTGGAGCTAT GGAAGAGGTA 
CCGTTAAAGC CTGGGGGTGG AAGTATTCTT GTGACACAAA ATAACAAAGC GGAGTACGTC 
CAGCTGGTTA CTGAACTCCG GATGACAAGA GCCATTCAGC CTCAGATCAA TGCGTTTTTA 
CAGGGTTTCC ATATGTTCAT CCCTCCTTCC CTCATACAGC TGTTCGACGA GTATGAATTG 
GAGCTACTGC TCTCTGGCAT GCCGGAAATT GACGTGAATG ATTGGATCAA AAATACAGAA 
TACACAAGTG GCTATGAAAG GGAAGATCCA GTTATTCAGT GGTTCTGGGA GGTTGTGGAG 
GACATGACTC AAGAAGAGCG GGTCCTTCTC TTACAGTTCG TGACTGGCAG TTCCAGGGTC 
CCGCATGGTG GCTTTGCCAA TATCATGGGT GGCAGTGGTT TGCAAAACTT TACAATTGCT 
GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCCAGCA CGTGCATCAA CATGCTCAAG 
TTACCTGAAT ACCCAAGTAA AGAGATCCTC AAGGACAGAC TCCTCGTAGC TCTGCATTGT 
GGCAGCTACG GCTATACAAT GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hace1 ( XM_017601694.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457183.1 Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa60132
Clone ID Related Accession (Same CDS sequence)	XM_017601694.1
Accession Version	XM_017601694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HACE1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAT TACTGGAGTA CAGTGCAGAC GTCAACATTT GCAATAATGA AGGCCTTACC 
GCAATCCACT GGCTGGCTGT GAATGGAAGG ACAGAGCTAC TCCATGACCT GGTGCAGCAC 
GTCACGGATG TGGATGTGGA GGATGCAATG GGGCAGACAG CATTACATGT GGCCTGCCAG 
AACGGGCACA AAACGACCGT GCAGTGTCTG CTGGACAGCG GTGCTGACAT CAACCGGCCG 
AATGTGGCAG GAGCTACGCC ACTGTACTTT GCTTGCAGTC ATGGCCAGAG AGATACTGCG 
CAGATTCTTC TATTACGAGG AGCCAAATAT TTACCAGATA AAAACGGAGT AACCCCTCTG 
GACCTGTGTG TGCAGGGTGG CTATGGACAG ACTTGCGAAG TGTTAATTCA GTATCACCCC 
AGGCTTTTCC AGACGATTGT TCAAATGACG CAGAATGAAG ACCTTCGGGA GAACATGTTA 
CGGCAAGTTC TGCAGCATTT GTCTCAGCAG AGTGAAAGCC AGTACCTAAA GATCCTGACA 
GGTCTTGCTG AAGTTGCTAC AACAAATGGC CATAAACTGC TTAGTGTGTT CCGGTTGGTC 
TCCAGTCTGT CCAGCAGCTA CGAGGCGCAG ATGAAGAGCC TCCTGCGGAT CGTGAGGATC 
TTCTGCCACG TCTTCCGCAT TGGCCCCTCG TCTCCCAGTA ACGGCATGGA TATGGGCTAC 
AATGGGAATA AGACTCCAAG GAGCCAGGTG TTCAAGCCTT TGGAATTGCT TTGGCACTCT 
CTGGATGAGT GGTTGGTTTT AATAGCCACA GAATTAATGA AGAACAAAGA GGACTCAGCG 
GATATCACCT CCATTTTACT GAAACAGAAA GGCCAGGATC AAGAGGCCTC TTGTATTTCA 
GCGTTTGAGC CACCAGGACC TGGGAGCTAT GAACGCCTGT CCACTGGTCC CGGGGAATCC 
AAACCAGATG TGCTTGCCGG GAAGCAGGAA GCCAGTGCGG ACTGTCAGGA TGTCATTTCT 
GTGACGGCCA ACCGGCTAAG TGCTGTCATT CAAGCCTTTT ACATGTGCTG CTCCTGTCAG 
ATGCCTCCAG GAATGACGTC GCCTCGGTTC ATTGAGTTTG TCTGCAAGCA TGATGAAGTG 
CTAAAGTGCT TTGTGAATAG AAATCCCAAA ATTATATTTG ACCATTTTCA CTTTCTTCTT 
GAATGTCCTG AACTGATGTC GAGATTCATG CACATCATAA AGGCACAGCC ATTCAAAGAC 
CGCTGTGAAT GGTTCTATGA GCATTTGCAC TCAGGACAGC CAGACTCAGA CATGGTGCAC 
AGACCAGTGA GTGAAAACGA CATTCTCCTG GTTCACAGAG ATTCTATTTT TAGGAGTAGC 
TGTGAAATCG TATCAAAGGC CAACTGTGCA AAGCTGAAGC AGGGGATCGC TGTGCGCTTT 
CATGGAGAGG AAGGCATGGG TCAAGGTGTT GTACGTGAAT GGTTTGATAT TCTGTCTAAT 
GAGATAGTCA ATCCTGACTA TGCACTGTTT ACCCAGTCAG CAGATGGAAC AACTTTTCAG 
CCCAATAGCA ACTCCTATGT CAATCCTGAT CACTTGAACT ATTTCCGGTT TGCTGGACAG 
ATCTTGGGAT TAGCTTTGAA TCACAGGCAG TTGGTCAATA TTTACTTCAC ACGATCCTTC 
TACAAACACA TTCTTGGTAT TCCTGTGAAT TACCAAGATG TAGCGTCTAT CGATCCAGAA 
TATGCCAAAA ATCTGCAGTG GATTTTAGAC AATGACATTA GTGACCTGGG TCTAGAACTG 
ACTTTCTCTG TTGAGACTGA TGTGTTTGGA GCTATGGAAG AGGTACCGTT AAAGCCTGGG 
GGTGGAAGTA TTCTTGTGAC ACAAAATAAC AAAGCGGAGT ACGTCCAGCT GGTTACTGAA 
CTCCGGATGA CAAGAGCCAT TCAGCCTCAG ATCAATGCGT TTTTACAGGG TTTCCATATG 
TTCATCCCTC CTTCCCTCAT ACAGCTGTTC GACGAGTATG AATTGGAGCT ACTGCTCTCT 
GGCATGCCGG AAATTGACGT GAATGATTGG ATCAAAAATA CAGAATACAC AAGTGGCTAT 
GAAAGGGAAG ATCCAGTTAT TCAGTGGTTC TGGGAGGTTG TGGAGGACAT GACTCAAGAA 
GAGCGGGTCC TTCTCTTACA GTTCGTGACT GGCAGTTCCA GGGTCCCGCA TGGTGGCTTT 
GCCAATATCA TGGGTGGCAG TGGTTTGCAA AACTTTACAA TTGCTGCTGT GCCATATACT 
CCAAATCTTT TACCAACTTC CAGCACGTGC ATCAACATGC TCAAGTTACC TGAATACCCA 
AGTAAAGAGA TCCTCAAGGA CAGACTCCTC GTAGCTCTGC ATTGTGGCAG CTACGGCTAT 
ACAATGGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457183.1
CDS	1051..3462
Translation	MSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVAGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHLSQQSESQYLKILTGLAEVATTNGHKLLSVFRLVSSLSSSYEAQMKSLLRIVRIFCHVFRIGPSSPSNGMDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKEDSADITSILLKQKGQDQEASCISAFEPPGPGSYERLSTGPGESKPDVLAGKQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVSENDILLVHRDSIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDMTQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_017601694.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601694.1

ORF Insert Sequence:

ATGAGTAAAT TACTGGAGTA CAGTGCAGAC GTCAACATTT GCAATAATGA AGGCCTTACC 
GCAATCCACT GGCTGGCTGT GAATGGAAGG ACAGAGCTAC TCCATGACCT GGTGCAGCAC 
GTCACGGATG TGGATGTGGA GGATGCAATG GGGCAGACAG CATTACATGT GGCCTGCCAG 
AACGGGCACA AAACGACCGT GCAGTGTCTG CTGGACAGCG GTGCTGACAT CAACCGGCCG 
AATGTGGCAG GAGCTACGCC ACTGTACTTT GCTTGCAGTC ATGGCCAGAG AGATACTGCG 
CAGATTCTTC TATTACGAGG AGCCAAATAT TTACCAGATA AAAACGGAGT AACCCCTCTG 
GACCTGTGTG TGCAGGGTGG CTATGGACAG ACTTGCGAAG TGTTAATTCA GTATCACCCC 
AGGCTTTTCC AGACGATTGT TCAAATGACG CAGAATGAAG ACCTTCGGGA GAACATGTTA 
CGGCAAGTTC TGCAGCATTT GTCTCAGCAG AGTGAAAGCC AGTACCTAAA GATCCTGACA 
GGTCTTGCTG AAGTTGCTAC AACAAATGGC CATAAACTGC TTAGTGTGTT CCGGTTGGTC 
TCCAGTCTGT CCAGCAGCTA CGAGGCGCAG ATGAAGAGCC TCCTGCGGAT CGTGAGGATC 
TTCTGCCACG TCTTCCGCAT TGGCCCCTCG TCTCCCAGTA ACGGCATGGA TATGGGCTAC 
AATGGGAATA AGACTCCAAG GAGCCAGGTG TTCAAGCCTT TGGAATTGCT TTGGCACTCT 
CTGGATGAGT GGTTGGTTTT AATAGCCACA GAATTAATGA AGAACAAAGA GGACTCAGCG 
GATATCACCT CCATTTTACT GAAACAGAAA GGCCAGGATC AAGAGGCCTC TTGTATTTCA 
GCGTTTGAGC CACCAGGACC TGGGAGCTAT GAACGCCTGT CCACTGGTCC CGGGGAATCC 
AAACCAGATG TGCTTGCCGG GAAGCAGGAA GCCAGTGCGG ACTGTCAGGA TGTCATTTCT 
GTGACGGCCA ACCGGCTAAG TGCTGTCATT CAAGCCTTTT ACATGTGCTG CTCCTGTCAG 
ATGCCTCCAG GAATGACGTC GCCTCGGTTC ATTGAGTTTG TCTGCAAGCA TGATGAAGTG 
CTAAAGTGCT TTGTGAATAG AAATCCCAAA ATTATATTTG ACCATTTTCA CTTTCTTCTT 
GAATGTCCTG AACTGATGTC GAGATTCATG CACATCATAA AGGCACAGCC ATTCAAAGAC 
CGCTGTGAAT GGTTCTATGA GCATTTGCAC TCAGGACAGC CAGACTCAGA CATGGTGCAC 
AGACCAGTGA GTGAAAACGA CATTCTCCTG GTTCACAGAG ATTCTATTTT TAGGAGTAGC 
TGTGAAATCG TATCAAAGGC CAACTGTGCA AAGCTGAAGC AGGGGATCGC TGTGCGCTTT 
CATGGAGAGG AAGGCATGGG TCAAGGTGTT GTACGTGAAT GGTTTGATAT TCTGTCTAAT 
GAGATAGTCA ATCCTGACTA TGCACTGTTT ACCCAGTCAG CAGATGGAAC AACTTTTCAG 
CCCAATAGCA ACTCCTATGT CAATCCTGAT CACTTGAACT ATTTCCGGTT TGCTGGACAG 
ATCTTGGGAT TAGCTTTGAA TCACAGGCAG TTGGTCAATA TTTACTTCAC ACGATCCTTC 
TACAAACACA TTCTTGGTAT TCCTGTGAAT TACCAAGATG TAGCGTCTAT CGATCCAGAA 
TATGCCAAAA ATCTGCAGTG GATTTTAGAC AATGACATTA GTGACCTGGG TCTAGAACTG 
ACTTTCTCTG TTGAGACTGA TGTGTTTGGA GCTATGGAAG AGGTACCGTT AAAGCCTGGG 
GGTGGAAGTA TTCTTGTGAC ACAAAATAAC AAAGCGGAGT ACGTCCAGCT GGTTACTGAA 
CTCCGGATGA CAAGAGCCAT TCAGCCTCAG ATCAATGCGT TTTTACAGGG TTTCCATATG 
TTCATCCCTC CTTCCCTCAT ACAGCTGTTC GACGAGTATG AATTGGAGCT ACTGCTCTCT 
GGCATGCCGG AAATTGACGT GAATGATTGG ATCAAAAATA CAGAATACAC AAGTGGCTAT 
GAAAGGGAAG ATCCAGTTAT TCAGTGGTTC TGGGAGGTTG TGGAGGACAT GACTCAAGAA 
GAGCGGGTCC TTCTCTTACA GTTCGTGACT GGCAGTTCCA GGGTCCCGCA TGGTGGCTTT 
GCCAATATCA TGGGTGGCAG TGGTTTGCAA AACTTTACAA TTGCTGCTGT GCCATATACT 
CCAAATCTTT TACCAACTTC CAGCACGTGC ATCAACATGC TCAAGTTACC TGAATACCCA 
AGTAAAGAGA TCCTCAAGGA CAGACTCCTC GTAGCTCTGC ATTGTGGCAG CTACGGCTAT 
ACAATGGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hace1 ( XM_017601695.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457184.1 Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa60133
Clone ID Related Accession (Same CDS sequence)	XM_017601695.1
Accession Version	XM_017601695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HACE1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAC AGAGCTACTC CATGACCTGG TGCAGCACGT CACGGATGTG GATGTGGAGG 
ATGCAATGGG GCAGACAGCA TTACATGTGG CCTGCCAGAA CGGGCACAAA ACGTCATGGC 
CAGAGAGATA CTGCGCAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAC 
GGAGTAACCC CTCTGGACCT GTGTGTGCAG GGTGGCTATG GACAGACTTG CGAAGTGTTA 
ATTCAGTATC ACCCCAGGCT TTTCCAGACG ATTGTTCAAA TGACGCAGAA TGAAGACCTT 
CGGGAGAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAGAGTGA AAGCCAGTAC 
CTAAAGATCC TGACAGGTCT TGCTGAAGTT GCTACAACAA ATGGCCATAA ACTGCTTAGT 
CTGTCCAGCA GCTACGAGGC GCAGATGAAG AGCCTCCTGC GGATCGTGAG GATCTTCTGC 
CACGTCTTCC GCATTGGCCC CTCGTCTCCC AGTAACGGCA TGGATATGGG CTACAATGGG 
AATAAGACTC CAAGGAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCTCTGGAT 
GAGTGGTTGG TTTTAATAGC CACAGAATTA ATGAAGAACA AAGAGGACTC AGCGGATATC 
ACCTCCATTT TACTGAAACA GAAAGGCCAG GATCAAGAGG CCTCTTGTAT TTCAGCGTTT 
GAGCCACCAG GACCTGGGAG CTATGAACGC CTGTCCACTG GTCCCGGGGA ATCCAAACCA 
GATGTGCTTG CCGGGAAGCA GGAAGCCAGT GCGGACTGTC AGGATGTCAT TTCTGTGACG 
GCCAACCGGC TAAGTGCTGT CATTCAAGCC TTTTACATGT GCTGCTCCTG TCAGATGCCT 
CCAGGAATGA CGTCGCCTCG GTTCATTGAG TTTGTCTGCA AGCATGATGA AGTGCTAAAG 
TGCTTTGTGA ATAGAAATCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT 
CCTGAACTGA TGTCGAGATT CATGCACATC ATAAAGGCAC AGCCATTCAA AGACCGCTGT 
GAATGGTTCT ATGAGCATTT GCACTCAGGA CAGCCAGACT CAGACATGGT GCACAGACCA 
GTGAGTGAAA ACGACATTCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
ATCGTATCAA AGGCCAACTG TGCAAAGCTG AAGCAGGGGA TCGCTGTGCG CTTTCATGGA 
GAGGAAGGCA TGGGTCAAGG TGTTGTACGT GAATGGTTTG ATATTCTGTC TAATGAGATA 
GTCAATCCTG ACTATGCACT GTTTACCCAG TCAGCAGATG GAACAACTTT TCAGCCCAAT 
AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG 
GGATTAGCTT TGAATCACAG GCAGTTGGTC AATATTTACT TCACACGATC CTTCTACAAA 
CACATTCTTG GTATTCCTGT GAATTACCAA GATGTAGCGT CTATCGATCC AGAATATGCC 
AAAAATCTGC AGTGGATTTT AGACAATGAC ATTAGTGACC TGGGTCTAGA ACTGACTTTC 
TCTGTTGAGA CTGATGTGTT TGGAGCTATG GAAGAGGTAC CGTTAAAGCC TGGGGGTGGA 
AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTACGTCC AGCTGGTTAC TGAACTCCGG 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCGTTTTTAC AGGGTTTCCA TATGTTCATC 
CCTCCTTCCC TCATACAGCT GTTCGACGAG TATGAATTGG AGCTACTGCT CTCTGGCATG 
CCGGAAATTG ACGTGAATGA TTGGATCAAA AATACAGAAT ACACAAGTGG CTATGAAAGG 
GAAGATCCAG TTATTCAGTG GTTCTGGGAG GTTGTGGAGG ACATGACTCA AGAAGAGCGG 
GTCCTTCTCT TACAGTTCGT GACTGGCAGT TCCAGGGTCC CGCATGGTGG CTTTGCCAAT 
ATCATGGGTG GCAGTGGTTT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCCAGCAC GTGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAGATCCTCA AGGACAGACT CCTCGTAGCT CTGCATTGTG GCAGCTACGG CTATACAATG 
GCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457184.1
CDS	96..2321
Translation	MEGQSYSMTWCSTSRMWMWRMQWGRQHYMWPARTGTKRHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHLSQQSESQYLKILTGLAEVATTNGHKLLSLSSSYEAQMKSLLRIVRIFCHVFRIGPSSPSNGMDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKEDSADITSILLKQKGQDQEASCISAFEPPGPGSYERLSTGPGESKPDVLAGKQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVSENDILLVHRDSIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDMTQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_017601695.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601695.1

ORF Insert Sequence:

ATGGAAGGAC AGAGCTACTC CATGACCTGG TGCAGCACGT CACGGATGTG GATGTGGAGG 
ATGCAATGGG GCAGACAGCA TTACATGTGG CCTGCCAGAA CGGGCACAAA ACGTCATGGC 
CAGAGAGATA CTGCGCAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAC 
GGAGTAACCC CTCTGGACCT GTGTGTGCAG GGTGGCTATG GACAGACTTG CGAAGTGTTA 
ATTCAGTATC ACCCCAGGCT TTTCCAGACG ATTGTTCAAA TGACGCAGAA TGAAGACCTT 
CGGGAGAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAGAGTGA AAGCCAGTAC 
CTAAAGATCC TGACAGGTCT TGCTGAAGTT GCTACAACAA ATGGCCATAA ACTGCTTAGT 
CTGTCCAGCA GCTACGAGGC GCAGATGAAG AGCCTCCTGC GGATCGTGAG GATCTTCTGC 
CACGTCTTCC GCATTGGCCC CTCGTCTCCC AGTAACGGCA TGGATATGGG CTACAATGGG 
AATAAGACTC CAAGGAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCTCTGGAT 
GAGTGGTTGG TTTTAATAGC CACAGAATTA ATGAAGAACA AAGAGGACTC AGCGGATATC 
ACCTCCATTT TACTGAAACA GAAAGGCCAG GATCAAGAGG CCTCTTGTAT TTCAGCGTTT 
GAGCCACCAG GACCTGGGAG CTATGAACGC CTGTCCACTG GTCCCGGGGA ATCCAAACCA 
GATGTGCTTG CCGGGAAGCA GGAAGCCAGT GCGGACTGTC AGGATGTCAT TTCTGTGACG 
GCCAACCGGC TAAGTGCTGT CATTCAAGCC TTTTACATGT GCTGCTCCTG TCAGATGCCT 
CCAGGAATGA CGTCGCCTCG GTTCATTGAG TTTGTCTGCA AGCATGATGA AGTGCTAAAG 
TGCTTTGTGA ATAGAAATCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT 
CCTGAACTGA TGTCGAGATT CATGCACATC ATAAAGGCAC AGCCATTCAA AGACCGCTGT 
GAATGGTTCT ATGAGCATTT GCACTCAGGA CAGCCAGACT CAGACATGGT GCACAGACCA 
GTGAGTGAAA ACGACATTCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
ATCGTATCAA AGGCCAACTG TGCAAAGCTG AAGCAGGGGA TCGCTGTGCG CTTTCATGGA 
GAGGAAGGCA TGGGTCAAGG TGTTGTACGT GAATGGTTTG ATATTCTGTC TAATGAGATA 
GTCAATCCTG ACTATGCACT GTTTACCCAG TCAGCAGATG GAACAACTTT TCAGCCCAAT 
AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG 
GGATTAGCTT TGAATCACAG GCAGTTGGTC AATATTTACT TCACACGATC CTTCTACAAA 
CACATTCTTG GTATTCCTGT GAATTACCAA GATGTAGCGT CTATCGATCC AGAATATGCC 
AAAAATCTGC AGTGGATTTT AGACAATGAC ATTAGTGACC TGGGTCTAGA ACTGACTTTC 
TCTGTTGAGA CTGATGTGTT TGGAGCTATG GAAGAGGTAC CGTTAAAGCC TGGGGGTGGA 
AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTACGTCC AGCTGGTTAC TGAACTCCGG 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCGTTTTTAC AGGGTTTCCA TATGTTCATC 
CCTCCTTCCC TCATACAGCT GTTCGACGAG TATGAATTGG AGCTACTGCT CTCTGGCATG 
CCGGAAATTG ACGTGAATGA TTGGATCAAA AATACAGAAT ACACAAGTGG CTATGAAAGG 
GAAGATCCAG TTATTCAGTG GTTCTGGGAG GTTGTGGAGG ACATGACTCA AGAAGAGCGG 
GTCCTTCTCT TACAGTTCGT GACTGGCAGT TCCAGGGTCC CGCATGGTGG CTTTGCCAAT 
ATCATGGGTG GCAGTGGTTT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCCAGCAC GTGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAGATCCTCA AGGACAGACT CCTCGTAGCT CTGCATTGTG GCAGCTACGG CTATACAATG 
GCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The E3 ubiquitin-ligase HACE1 catalyzes the ubiquitylation of active Rac1.
Developmental cell21(5)959-65(2011 Nov)
Torrino S,Visvikis O,Doye A,Boyer L,Stefani C,Munro P,Bertoglio J,Gacon G,Mettouchi A,Lemichez E

The ubiquitin ligase HACE1 regulates Golgi membrane dynamics during the cell cycle.
Nature communications2501(2011 Oct)
Tang D,Xiang Y,De Renzis S,Rink J,Zheng G,Zerial M,Wang Y

Differential expression of a novel ankyrin containing E3 ubiquitin-protein ligase, Hace1, in sporadic Wilms' tumor versus normal kidney.
Human molecular genetics13(18)2061-74(2004 Sep)
Anglesio MS,Evdokimova V,Melnyk N,Zhang L,Fernandez CV,Grundy PE,Leach S,Marra MA,Brooks-Wilson AR,Penninger J,Sorensen PH

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Hace1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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