HACE1 cDNA ORF clone, Gallus gallus(chicken)

The following HACE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HACE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa00114 NM_001278155.1
Latest version!
Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OGa33965 XM_015284529.2
Latest version!
Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa33966 XM_015284530.2
Latest version!
Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa33967 XM_015284531.1
Latest version!
Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OGa33966 XM_015284530.1 Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa33965 XM_015284529.1 Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa00114
    Clone ID Related Accession (Same CDS sequence) NM_001278155.1
    Accession Version NM_001278155.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-09-22
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HACE1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000003.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201361, SAMEA2201363 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGAGAG CCATGGAGCA GCTCAACCGC CTCACCAGGT CCTTGCGCCG CGCCCGCACC 
    GTGGAGCTGC CGGACGATAA CGAAACTGCT GTTTATACAC TAATGCCAAT GGTGATGGCT
    GACCAGCACA GGTCTGTCTC AGAGCTGCTA TCAAATTCAA AATTTGATGT GAATTATGCA
    TTTGGACGTG TGAAGAGAAG TTTGCTTCAT ATTGCAGCAA ACTGTGGCTC AGTTGAATGC
    CTTGTTCTGT TATTAAAAAA AGGAGCAAAT CCAAACTATC AGGACATCTC AGGATGCACA
    CCTCTCCATT TAGCAGCAAG GAATGGCCAG AAAAAATGCA TGAGCAAGCT GCTGGAATAC
    AGTGCAGATG TCAACATTTG TAATAACGAA GGCTTGACTG CAATTCATTG GTTGGCTGTC
    AATGGACGGA CAGAATTGCT TCATGATCTT GTGCAGCATG TCAGTAATGT AGATGTTGAA
    GATGCAATGG GACAGACAGC CCTTCATGTA GCTTGTCAAA ATGGACACAA GACAACAGTA
    CAGTGTCTGC TGGACAGTGG TGCAGATATA AATAGGCCCA ATGTATCAGG TGCAACTCCA
    CTCTATTTTG CTTGCAGCCA TGGTCAGAGA GACACTGCAC AAATTCTTTT GATGAGAGGA
    GCCAAATACT TACCAGACAA AAATGGCATT ACTCCACTGG ATCTCTGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT CTTAATACAG TACCATCCTC GACTTTTCCA GACAATAATT
    CAAATGACTC AGAATGAAGA TCTACGGGAA AACATGTTGC GGCAAGTTCT GGAACATTTG
    TCTCAGCAGA GTGAAAGCCA GTATCTTAAA ATCCTGACAA GTCTTGCTGA AGTTGCTACA
    ACTAATGGTC ACAAACTACT TAGCTTGTCA AGTAATTATG AAGCTCAAAT GAAGAGTCTC
    TTAAGGATCG TGAGGATATT TTGCCATGTG TTTCGCATTG GCCCATCCTC TCCCAGTAAT
    GGAAATGACA TGGGCTACAA TGGAAATAAA ACTCCAAGAA GTCAGGTGTT CAAGGTCAGA
    AAAGTATATG ATGTTGTTAG GAAGATAGAC GTTAAAGAGA TGAATTTCAC AAAGCATGCA
    TTCATTAATC AGACATCTCA TGAACAGGAA CCACTAGAAC TGCTGTGGCA CTCATTAGAC
    GAATGGCTAG TTCTTATAGC CACAGAGCTG ATGAAAAACA AAAGGGACTC TGCCAACATT
    ACTTCTATTT TGCTGAAACA AAAAGGACCA GATCACCAGG ATGCTACTCC TACGCCTTCC
    TTTGCCGCTG CAGGAACAGA GAGCAGAAAA GAGCTATCCA CTGACACAGG GGACTCAAAG
    ACATATGAAG TTGCGGGGAA GCAGGAAGCT TATGCTGATT GCCAGGATGT TATCTCCATG
    ACAGCAAACA GGCTAAGTGC TGTCATTCAA GCATTTTATA TGTGCTGCTC CTGTCAGATG
    CCTCAGGGGA TGACGTCACC TCGTTTTATA GAATTTGTTT GTAAACACGA TGATGTCCTT
    AAGTGTTTTG TTAACCGGAA TCCCAAAATC ATTTTTGATC ACTTTCATTT CCTTCTTGAG
    TGTCCTGAAC TAATGTCCAG ATTTATGCAC ATCATAAAGG CTCAGCCATT TAAAGATCGT
    TGTGAATGGT TCTATGAACA TCTGCATTCA GGGCAACCAG ACTCAGACAT GGTGCACAGA
    CCTGTCAATG AAAATGACAT CCTTCTGGTT CACAGAGATT CTATTTTTAG GAGTAGCTGT
    GAAGTTGTCT CTAAAGCAAA TTGTGCAAAA CTAAAGCAAG GGATTGCTGT GCGATTTCAC
    GGAGAAGAAG GCATGGGACA GGGTGTGGTG CGTGAGTGGT TTGATATCTT ATCCAGTGAA
    ATAGTTAACC CAGATTATGC CTTATTTACC CAGTCAGCTG ATGGAACAAC CTTCCAGCCA
    AACAGCAACT CTTCTGTAAA CCCAGATCAT TTGAACTACT TCCGCTTTGC AGGCCAGATC
    CTGGGATTAG CTCTCAATCA CAGGCAGCTG GTGAACATTT ACTTCACAAG ATCATTCTAC
    AAGCATATTC TTGGTATACC TGTAAATTAT CAGGATGTAG CATCTATTGA TCCAGAGTAT
    GCAAAGAATT TGCAGTGGAT TTTAGATAAT GATATCAGTG ATCTGGGTTT AGAGCTGACT
    TTTTCTGTTG AGACTGACGT ATTTGGAGCA ATGGAAGAAG TGCCATTAAA GCCTGGGGGT
    GCGAGTATAC TTGTTACACA GGAAAACAAG GCTGAGTATG TCCAGCTTGT CACTGAACTC
    AGAATGACAA GAGCCATTCA ACCTCAGATA AATGCCTTTT TACAAGGCTT CCATATGTTC
    ATTCCACCAT CTTTGATTCA ACTTTTTGAT GAATATGAAC TGGAGCTTTT GTTGTCTGGT
    ATGCCGGAAA TTGATGTGAA TGATTGGTTA AAAAATACAG AATATACCAG TGGCTATGAG
    AGAGGAGACC AAGTAATCCA GTGGTTCTGG GATGTTGTGG AAGAATTAAC TCAGGAAGAG
    AGAGTACTAC TATTACAGTT TGTTACTGGC AGTTCCAGAG TGCCTCATGG TGGCTTTGCC
    CATATAATGG GTGGGAGCGG ATTGCAAAAT TTTACAATTG CTGCTGTGCC ATATACTGCG
    AACCTCTTAC CAACATCGAG CACGTGTATC AACATGCTCA AGTTACCTGA ATACCCAAGT
    AAAGAAATCC TGAAGGACAG GCTTCTTGTG GCATTGCACT GTGGAAGCTA TGGTTACACA
    ATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001265084.1
    CDS96..2924
    Translation

    Target ORF information:

    RefSeq Version NM_001278155.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001278155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGAGAG CCATGGAGCA GCTCAACCGC CTCACCAGGT CCTTGCGCCG CGCCCGCACC 
    GTGGAGCTGC CGGACGATAA CGAAACTGCT GTTTATACAC TAATGCCAAT GGTGATGGCT
    GACCAGCACA GGTCTGTCTC AGAGCTGCTA TCAAATTCAA AATTTGATGT GAATTATGCA
    TTTGGACGTG TGAAGAGAAG TTTGCTTCAT ATTGCAGCAA ACTGTGGCTC AGTTGAATGC
    CTTGTTCTGT TATTAAAAAA AGGAGCAAAT CCAAACTATC AGGACATCTC AGGATGCACA
    CCTCTCCATT TAGCAGCAAG GAATGGCCAG AAAAAATGCA TGAGCAAGCT GCTGGAATAC
    AGTGCAGATG TCAACATTTG TAATAACGAA GGCTTGACTG CAATTCATTG GTTGGCTGTC
    AATGGACGGA CAGAATTGCT TCATGATCTT GTGCAGCATG TCAGTAATGT AGATGTTGAA
    GATGCAATGG GACAGACAGC CCTTCATGTA GCTTGTCAAA ATGGACACAA GACAACAGTA
    CAGTGTCTGC TGGACAGTGG TGCAGATATA AATAGGCCCA ATGTATCAGG TGCAACTCCA
    CTCTATTTTG CTTGCAGCCA TGGTCAGAGA GACACTGCAC AAATTCTTTT GATGAGAGGA
    GCCAAATACT TACCAGACAA AAATGGCATT ACTCCACTGG ATCTCTGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT CTTAATACAG TACCATCCTC GACTTTTCCA GACAATAATT
    CAAATGACTC AGAATGAAGA TCTACGGGAA AACATGTTGC GGCAAGTTCT GGAACATTTG
    TCTCAGCAGA GTGAAAGCCA GTATCTTAAA ATCCTGACAA GTCTTGCTGA AGTTGCTACA
    ACTAATGGTC ACAAACTACT TAGCTTGTCA AGTAATTATG AAGCTCAAAT GAAGAGTCTC
    TTAAGGATCG TGAGGATATT TTGCCATGTG TTTCGCATTG GCCCATCCTC TCCCAGTAAT
    GGAAATGACA TGGGCTACAA TGGAAATAAA ACTCCAAGAA GTCAGGTGTT CAAGGTCAGA
    AAAGTATATG ATGTTGTTAG GAAGATAGAC GTTAAAGAGA TGAATTTCAC AAAGCATGCA
    TTCATTAATC AGACATCTCA TGAACAGGAA CCACTAGAAC TGCTGTGGCA CTCATTAGAC
    GAATGGCTAG TTCTTATAGC CACAGAGCTG ATGAAAAACA AAAGGGACTC TGCCAACATT
    ACTTCTATTT TGCTGAAACA AAAAGGACCA GATCACCAGG ATGCTACTCC TACGCCTTCC
    TTTGCCGCTG CAGGAACAGA GAGCAGAAAA GAGCTATCCA CTGACACAGG GGACTCAAAG
    ACATATGAAG TTGCGGGGAA GCAGGAAGCT TATGCTGATT GCCAGGATGT TATCTCCATG
    ACAGCAAACA GGCTAAGTGC TGTCATTCAA GCATTTTATA TGTGCTGCTC CTGTCAGATG
    CCTCAGGGGA TGACGTCACC TCGTTTTATA GAATTTGTTT GTAAACACGA TGATGTCCTT
    AAGTGTTTTG TTAACCGGAA TCCCAAAATC ATTTTTGATC ACTTTCATTT CCTTCTTGAG
    TGTCCTGAAC TAATGTCCAG ATTTATGCAC ATCATAAAGG CTCAGCCATT TAAAGATCGT
    TGTGAATGGT TCTATGAACA TCTGCATTCA GGGCAACCAG ACTCAGACAT GGTGCACAGA
    CCTGTCAATG AAAATGACAT CCTTCTGGTT CACAGAGATT CTATTTTTAG GAGTAGCTGT
    GAAGTTGTCT CTAAAGCAAA TTGTGCAAAA CTAAAGCAAG GGATTGCTGT GCGATTTCAC
    GGAGAAGAAG GCATGGGACA GGGTGTGGTG CGTGAGTGGT TTGATATCTT ATCCAGTGAA
    ATAGTTAACC CAGATTATGC CTTATTTACC CAGTCAGCTG ATGGAACAAC CTTCCAGCCA
    AACAGCAACT CTTCTGTAAA CCCAGATCAT TTGAACTACT TCCGCTTTGC AGGCCAGATC
    CTGGGATTAG CTCTCAATCA CAGGCAGCTG GTGAACATTT ACTTCACAAG ATCATTCTAC
    AAGCATATTC TTGGTATACC TGTAAATTAT CAGGATGTAG CATCTATTGA TCCAGAGTAT
    GCAAAGAATT TGCAGTGGAT TTTAGATAAT GATATCAGTG ATCTGGGTTT AGAGCTGACT
    TTTTCTGTTG AGACTGACGT ATTTGGAGCA ATGGAAGAAG TGCCATTAAA GCCTGGGGGT
    GCGAGTATAC TTGTTACACA GGAAAACAAG GCTGAGTATG TCCAGCTTGT CACTGAACTC
    AGAATGACAA GAGCCATTCA ACCTCAGATA AATGCCTTTT TACAAGGCTT CCATATGTTC
    ATTCCACCAT CTTTGATTCA ACTTTTTGAT GAATATGAAC TGGAGCTTTT GTTGTCTGGT
    ATGCCGGAAA TTGATGTGAA TGATTGGTTA AAAAATACAG AATATACCAG TGGCTATGAG
    AGAGGAGACC AAGTAATCCA GTGGTTCTGG GATGTTGTGG AAGAATTAAC TCAGGAAGAG
    AGAGTACTAC TATTACAGTT TGTTACTGGC AGTTCCAGAG TGCCTCATGG TGGCTTTGCC
    CATATAATGG GTGGGAGCGG ATTGCAAAAT TTTACAATTG CTGCTGTGCC ATATACTGCG
    AACCTCTTAC CAACATCGAG CACGTGTATC AACATGCTCA AGTTACCTGA ATACCCAAGT
    AAAGAAATCC TGAAGGACAG GCTTCTTGTG GCATTGCACT GTGGAAGCTA TGGTTACACA
    ATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33965
    Clone ID Related Accession (Same CDS sequence) XM_015284529.1 , XM_015284529.2
    Accession Version XM_015284529.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455841.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CCATGGAGCA GCTCAACCGC CTCACCAGGT CCTTGCGCCG CGCCCGCACC 
    GTGGAGCTGC CGGACGATAA CGAAACTGCT GTTTATACAC TAATGCCAAT GGTGATGGCT
    GACCAGCACA GGTCTGTCTC AGAGCTGCTA TCAAATTCAA AATTTGATGT GAATTATGCA
    TTTGGACGTG TGAAGAGAAG TTTGCTTCAT ATTGCAGCAA ACTGTGGCTC AGTTGAATGC
    CTTGTTCTGT TATTAAAAAA AGGAGCAAAT CCAAACTATC AGGACATCTC AGGATGCACA
    CCTCTCCATT TAGCAGCAAG GAATGGCCAG AAAAAATGCA TGAGCAAGCT GCTGGAATAC
    AGTGCAGATG TCAACATTTG TAATAACGAA GGCTTGACTG CAATTCATTG GTTGGCTGTC
    AATGGACGGA CAGAATTGCT TCATGATCTT GTGCAGCATG TCAGTAATGT AGATGTTGAA
    GATGCAATGG GACAGACAGC CCTTCATGTA GCTTGTCAAA ATGGACACAA GACAACAGTA
    CAGTGTCTGC TGGACAGTGG TGCAGATATA AATAGGCCCA ATGTATCAGG TGCAACTCCA
    CTCTATTTTG CTTGCAGCCA TGGTCAGAGA GACACTGCAC AAATTCTTTT GATGAGAGGA
    GCCAAATACT TACCAGACAA AAATGGCATT ACTCCACTGG ATCTCTGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT CTTAATACAG TACCATCCTC GACTTTTCCA GACAATAATT
    CAAATGACTC AGAATGAAGA TCTACGGGAA AACATGTTGC GGCAAGTTCT GGAACATTTG
    TCTCAGCAGA GTGAAAGCCA GTATCTTAAA ATCCTGACAA GTCTTGCTGA AGTTGCTACA
    ACTAATGGTC ACAAACTACT TAGCTTGTCA AGTAATTATG AAGCTCAAAT GAAGAGTCTC
    TTAAGGATCG TGAGGATATT TTGCCATGTG TTTCGCATTG GCCCATCCTC TCCCAGTAAT
    GGAAATGACA TGGGCTACAA TGGAAATAAA ACTCCAAGAA GTCAGGTGTT CAAGCCACTA
    GAACTGCTGT GGCACTCATT AGACGAATGG CTAGTTCTTA TAGCCACAGA GCTGATGAAA
    AACAAAAGGG ACTCTGCCAA CATTACTTCT ATTTTGCTGA AACAAAAAGG ACCAGATCAC
    CAGGATGCTA CTCCTACGCC TTCCTTTGCC GCTGCAGGAA CAGAGAGCAG AAAAGAGCTA
    TCCACTGACA CAGGGGACTC AAAGACATAT GAAGTTGCGG GGAAGCAGGA AGCTTATGCT
    GATTGCCAGG ATGTTATCTC CATGACAGCA AACAGGCTAA GTGCTGTCAT TCAAGCATTT
    TATATGTGCT GCTCCTGTCA GATGCCTCAG GGGATGACGT CACCTCGTTT TATAGAATTT
    GTTTGTAAAC ACGATGATGT CCTTAAGTGT TTTGTTAACC GGAATCCCAA AATCATTTTT
    GATCACTTTC ATTTCCTTCT TGAGTGTCCT GAACTAATGT CCAGATTTAT GCACATCATA
    AAGGCTCAGC CATTTAAAGA TCGTTGTGAA TGGTTCTATG AACATCTGCA TTCAGGGCAA
    CCAGACTCAG ACATGGTGCA CAGACCTGTC AATGAAAATG ACATCCTTCT GGTTCACAGA
    GATTCTATTT TTAGGAGTAG CTGTGAAGTT GTCTCTAAAG CAAATTGTGC AAAACTAAAG
    CAAGGGATTG CTGTGCGATT TCACGGAGAA GAAGGCATGG GACAGGGTGT GGTGCGTGAG
    TGGTTTGATA TCTTATCCAG TGAAATAGTT AACCCAGATT ATGCCTTATT TACCCAGTCA
    GCTGATGGAA CAACCTTCCA GCCAAACAGC AACTCTTCTG TAAACCCAGA TCATTTGAAC
    TACTTCCGCT TTGCAGGCCA GATCCTGGGA TTAGCTCTCA ATCACAGGCA GCTGGTGAAC
    ATTTACTTCA CAAGATCATT CTACAAGCAT ATTCTTGGTA TACCTGTAAA TTATCAGGAT
    GTAGCATCTA TTGATCCAGA GTATGCAAAG AATTTGCAGT GGATTTTAGA TAATGATATC
    AGTGATCTGG GTTTAGAGCT GACTTTTTCT GTTGAGACTG ACGTATTTGG AGCAATGGAA
    GAAGTGCCAT TAAAGCCTGG GGGTGCGAGT ATACTTGTTA CACAGGAAAA CAAGGCTGAG
    TATGTCCAGC TTGTCACTGA ACTCAGAATG ACAAGAGCCA TTCAACCTCA GATAAATGCC
    TTTTTACAAG GCTTCCATAT GTTCATTCCA CCATCTTTGA TTCAACTTTT TGATGAATAT
    GAACTGGAGC TTTTGTTGTC TGGTATGCCG GAAATTGATG TGAATGATTG GTTAAAAAAT
    ACAGAATATA CCAGTGGCTA TGAGAGAGGA GACCAAGTAA TCCAGTGGTT CTGGGATGTT
    GTGGAAGAAT TAACTCAGGA AGAGAGAGTA CTACTATTAC AGTTTGTTAC TGGCAGTTCC
    AGAGTGCCTC ATGGTGGCTT TGCCCATATA ATGGGTGGGA GCGGATTGCA AAATTTTACA
    ATTGCTGCTG TGCCATATAC TGCGAACCTC TTACCAACAT CGAGCACGTG TATCAACATG
    CTCAAGTTAC CTGAATACCC AAGTAAAGAA ATCCTGAAGG ACAGGCTTCT TGTGGCATTG
    CACTGTGGAA GCTATGGTTA CACAATGGCA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140015.1
    CDS96..2828
    Translation

    Target ORF information:

    RefSeq Version XM_015284529.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CCATGGAGCA GCTCAACCGC CTCACCAGGT CCTTGCGCCG CGCCCGCACC 
    GTGGAGCTGC CGGACGATAA CGAAACTGCT GTTTATACAC TAATGCCAAT GGTGATGGCT
    GACCAGCACA GGTCTGTCTC AGAGCTGCTA TCAAATTCAA AATTTGATGT GAATTATGCA
    TTTGGACGTG TGAAGAGAAG TTTGCTTCAT ATTGCAGCAA ACTGTGGCTC AGTTGAATGC
    CTTGTTCTGT TATTAAAAAA AGGAGCAAAT CCAAACTATC AGGACATCTC AGGATGCACA
    CCTCTCCATT TAGCAGCAAG GAATGGCCAG AAAAAATGCA TGAGCAAGCT GCTGGAATAC
    AGTGCAGATG TCAACATTTG TAATAACGAA GGCTTGACTG CAATTCATTG GTTGGCTGTC
    AATGGACGGA CAGAATTGCT TCATGATCTT GTGCAGCATG TCAGTAATGT AGATGTTGAA
    GATGCAATGG GACAGACAGC CCTTCATGTA GCTTGTCAAA ATGGACACAA GACAACAGTA
    CAGTGTCTGC TGGACAGTGG TGCAGATATA AATAGGCCCA ATGTATCAGG TGCAACTCCA
    CTCTATTTTG CTTGCAGCCA TGGTCAGAGA GACACTGCAC AAATTCTTTT GATGAGAGGA
    GCCAAATACT TACCAGACAA AAATGGCATT ACTCCACTGG ATCTCTGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT CTTAATACAG TACCATCCTC GACTTTTCCA GACAATAATT
    CAAATGACTC AGAATGAAGA TCTACGGGAA AACATGTTGC GGCAAGTTCT GGAACATTTG
    TCTCAGCAGA GTGAAAGCCA GTATCTTAAA ATCCTGACAA GTCTTGCTGA AGTTGCTACA
    ACTAATGGTC ACAAACTACT TAGCTTGTCA AGTAATTATG AAGCTCAAAT GAAGAGTCTC
    TTAAGGATCG TGAGGATATT TTGCCATGTG TTTCGCATTG GCCCATCCTC TCCCAGTAAT
    GGAAATGACA TGGGCTACAA TGGAAATAAA ACTCCAAGAA GTCAGGTGTT CAAGCCACTA
    GAACTGCTGT GGCACTCATT AGACGAATGG CTAGTTCTTA TAGCCACAGA GCTGATGAAA
    AACAAAAGGG ACTCTGCCAA CATTACTTCT ATTTTGCTGA AACAAAAAGG ACCAGATCAC
    CAGGATGCTA CTCCTACGCC TTCCTTTGCC GCTGCAGGAA CAGAGAGCAG AAAAGAGCTA
    TCCACTGACA CAGGGGACTC AAAGACATAT GAAGTTGCGG GGAAGCAGGA AGCTTATGCT
    GATTGCCAGG ATGTTATCTC CATGACAGCA AACAGGCTAA GTGCTGTCAT TCAAGCATTT
    TATATGTGCT GCTCCTGTCA GATGCCTCAG GGGATGACGT CACCTCGTTT TATAGAATTT
    GTTTGTAAAC ACGATGATGT CCTTAAGTGT TTTGTTAACC GGAATCCCAA AATCATTTTT
    GATCACTTTC ATTTCCTTCT TGAGTGTCCT GAACTAATGT CCAGATTTAT GCACATCATA
    AAGGCTCAGC CATTTAAAGA TCGTTGTGAA TGGTTCTATG AACATCTGCA TTCAGGGCAA
    CCAGACTCAG ACATGGTGCA CAGACCTGTC AATGAAAATG ACATCCTTCT GGTTCACAGA
    GATTCTATTT TTAGGAGTAG CTGTGAAGTT GTCTCTAAAG CAAATTGTGC AAAACTAAAG
    CAAGGGATTG CTGTGCGATT TCACGGAGAA GAAGGCATGG GACAGGGTGT GGTGCGTGAG
    TGGTTTGATA TCTTATCCAG TGAAATAGTT AACCCAGATT ATGCCTTATT TACCCAGTCA
    GCTGATGGAA CAACCTTCCA GCCAAACAGC AACTCTTCTG TAAACCCAGA TCATTTGAAC
    TACTTCCGCT TTGCAGGCCA GATCCTGGGA TTAGCTCTCA ATCACAGGCA GCTGGTGAAC
    ATTTACTTCA CAAGATCATT CTACAAGCAT ATTCTTGGTA TACCTGTAAA TTATCAGGAT
    GTAGCATCTA TTGATCCAGA GTATGCAAAG AATTTGCAGT GGATTTTAGA TAATGATATC
    AGTGATCTGG GTTTAGAGCT GACTTTTTCT GTTGAGACTG ACGTATTTGG AGCAATGGAA
    GAAGTGCCAT TAAAGCCTGG GGGTGCGAGT ATACTTGTTA CACAGGAAAA CAAGGCTGAG
    TATGTCCAGC TTGTCACTGA ACTCAGAATG ACAAGAGCCA TTCAACCTCA GATAAATGCC
    TTTTTACAAG GCTTCCATAT GTTCATTCCA CCATCTTTGA TTCAACTTTT TGATGAATAT
    GAACTGGAGC TTTTGTTGTC TGGTATGCCG GAAATTGATG TGAATGATTG GTTAAAAAAT
    ACAGAATATA CCAGTGGCTA TGAGAGAGGA GACCAAGTAA TCCAGTGGTT CTGGGATGTT
    GTGGAAGAAT TAACTCAGGA AGAGAGAGTA CTACTATTAC AGTTTGTTAC TGGCAGTTCC
    AGAGTGCCTC ATGGTGGCTT TGCCCATATA ATGGGTGGGA GCGGATTGCA AAATTTTACA
    ATTGCTGCTG TGCCATATAC TGCGAACCTC TTACCAACAT CGAGCACGTG TATCAACATG
    CTCAAGTTAC CTGAATACCC AAGTAAAGAA ATCCTGAAGG ACAGGCTTCT TGTGGCATTG
    CACTGTGGAA GCTATGGTTA CACAATGGCA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33965
    Clone ID Related Accession (Same CDS sequence) XM_015284529.1 , XM_015284529.2
    Accession Version XM_015284529.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CCATGGAGCA GCTCAACCGC CTCACCAGGT CCTTGCGCCG CGCCCGCACC 
    GTGGAGCTGC CGGACGATAA CGAAACTGCT GTTTATACAC TAATGCCAAT GGTGATGGCT
    GACCAGCACA GGTCTGTCTC AGAGCTGCTA TCAAATTCAA AATTTGATGT GAATTATGCA
    TTTGGACGTG TGAAGAGAAG TTTGCTTCAT ATTGCAGCAA ACTGTGGCTC AGTTGAATGC
    CTTGTTCTGT TATTAAAAAA AGGAGCAAAT CCAAACTATC AGGACATCTC AGGATGCACA
    CCTCTCCATT TAGCAGCAAG GAATGGCCAG AAAAAATGCA TGAGCAAGCT GCTGGAATAC
    AGTGCAGATG TCAACATTTG TAATAACGAA GGCTTGACTG CAATTCATTG GTTGGCTGTC
    AATGGACGGA CAGAATTGCT TCATGATCTT GTGCAGCATG TCAGTAATGT AGATGTTGAA
    GATGCAATGG GACAGACAGC CCTTCATGTA GCTTGTCAAA ATGGACACAA GACAACAGTA
    CAGTGTCTGC TGGACAGTGG TGCAGATATA AATAGGCCCA ATGTATCAGG TGCAACTCCA
    CTCTATTTTG CTTGCAGCCA TGGTCAGAGA GACACTGCAC AAATTCTTTT GATGAGAGGA
    GCCAAATACT TACCAGACAA AAATGGCATT ACTCCACTGG ATCTCTGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT CTTAATACAG TACCATCCTC GACTTTTCCA GACAATAATT
    CAAATGACTC AGAATGAAGA TCTACGGGAA AACATGTTGC GGCAAGTTCT GGAACATTTG
    TCTCAGCAGA GTGAAAGCCA GTATCTTAAA ATCCTGACAA GTCTTGCTGA AGTTGCTACA
    ACTAATGGTC ACAAACTACT TAGCTTGTCA AGTAATTATG AAGCTCAAAT GAAGAGTCTC
    TTAAGGATCG TGAGGATATT TTGCCATGTG TTTCGCATTG GCCCATCCTC TCCCAGTAAT
    GGAAATGACA TGGGCTACAA TGGAAATAAA ACTCCAAGAA GTCAGGTGTT CAAGCCACTA
    GAACTGCTGT GGCACTCATT AGACGAATGG CTAGTTCTTA TAGCCACAGA GCTGATGAAA
    AACAAAAGGG ACTCTGCCAA CATTACTTCT ATTTTGCTGA AACAAAAAGG ACCAGATCAC
    CAGGATGCTA CTCCTACGCC TTCCTTTGCC GCTGCAGGAA CAGAGAGCAG AAAAGAGCTA
    TCCACTGACA CAGGGGACTC AAAGACATAT GAAGTTGCGG GGAAGCAGGA AGCTTATGCT
    GATTGCCAGG ATGTTATCTC CATGACAGCA AACAGGCTAA GTGCTGTCAT TCAAGCATTT
    TATATGTGCT GCTCCTGTCA GATGCCTCAG GGGATGACGT CACCTCGTTT TATAGAATTT
    GTTTGTAAAC ACGATGATGT CCTTAAGTGT TTTGTTAACC GGAATCCCAA AATCATTTTT
    GATCACTTTC ATTTCCTTCT TGAGTGTCCT GAACTAATGT CCAGATTTAT GCACATCATA
    AAGGCTCAGC CATTTAAAGA TCGTTGTGAA TGGTTCTATG AACATCTGCA TTCAGGGCAA
    CCAGACTCAG ACATGGTGCA CAGACCTGTC AATGAAAATG ACATCCTTCT GGTTCACAGA
    GATTCTATTT TTAGGAGTAG CTGTGAAGTT GTCTCTAAAG CAAATTGTGC AAAACTAAAG
    CAAGGGATTG CTGTGCGATT TCACGGAGAA GAAGGCATGG GACAGGGTGT GGTGCGTGAG
    TGGTTTGATA TCTTATCCAG TGAAATAGTT AACCCAGATT ATGCCTTATT TACCCAGTCA
    GCTGATGGAA CAACCTTCCA GCCAAACAGC AACTCTTCTG TAAACCCAGA TCATTTGAAC
    TACTTCCGCT TTGCAGGCCA GATCCTGGGA TTAGCTCTCA ATCACAGGCA GCTGGTGAAC
    ATTTACTTCA CAAGATCATT CTACAAGCAT ATTCTTGGTA TACCTGTAAA TTATCAGGAT
    GTAGCATCTA TTGATCCAGA GTATGCAAAG AATTTGCAGT GGATTTTAGA TAATGATATC
    AGTGATCTGG GTTTAGAGCT GACTTTTTCT GTTGAGACTG ACGTATTTGG AGCAATGGAA
    GAAGTGCCAT TAAAGCCTGG GGGTGCGAGT ATACTTGTTA CACAGGAAAA CAAGGCTGAG
    TATGTCCAGC TTGTCACTGA ACTCAGAATG ACAAGAGCCA TTCAACCTCA GATAAATGCC
    TTTTTACAAG GCTTCCATAT GTTCATTCCA CCATCTTTGA TTCAACTTTT TGATGAATAT
    GAACTGGAGC TTTTGTTGTC TGGTATGCCG GAAATTGATG TGAATGATTG GTTAAAAAAT
    ACAGAATATA CCAGTGGCTA TGAGAGAGGA GACCAAGTAA TCCAGTGGTT CTGGGATGTT
    GTGGAAGAAT TAACTCAGGA AGAGAGAGTA CTACTATTAC AGTTTGTTAC TGGCAGTTCC
    AGAGTGCCTC ATGGTGGCTT TGCCCATATA ATGGGTGGGA GCGGATTGCA AAATTTTACA
    ATTGCTGCTG TGCCATATAC TGCGAACCTC TTACCAACAT CGAGCACGTG TATCAACATG
    CTCAAGTTAC CTGAATACCC AAGTAAAGAA ATCCTGAAGG ACAGGCTTCT TGTGGCATTG
    CACTGTGGAA GCTATGGTTA CACAATGGCA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140015.1
    CDS96..2828
    Translation

    Target ORF information:

    RefSeq Version XM_015284529.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284529.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CCATGGAGCA GCTCAACCGC CTCACCAGGT CCTTGCGCCG CGCCCGCACC 
    GTGGAGCTGC CGGACGATAA CGAAACTGCT GTTTATACAC TAATGCCAAT GGTGATGGCT
    GACCAGCACA GGTCTGTCTC AGAGCTGCTA TCAAATTCAA AATTTGATGT GAATTATGCA
    TTTGGACGTG TGAAGAGAAG TTTGCTTCAT ATTGCAGCAA ACTGTGGCTC AGTTGAATGC
    CTTGTTCTGT TATTAAAAAA AGGAGCAAAT CCAAACTATC AGGACATCTC AGGATGCACA
    CCTCTCCATT TAGCAGCAAG GAATGGCCAG AAAAAATGCA TGAGCAAGCT GCTGGAATAC
    AGTGCAGATG TCAACATTTG TAATAACGAA GGCTTGACTG CAATTCATTG GTTGGCTGTC
    AATGGACGGA CAGAATTGCT TCATGATCTT GTGCAGCATG TCAGTAATGT AGATGTTGAA
    GATGCAATGG GACAGACAGC CCTTCATGTA GCTTGTCAAA ATGGACACAA GACAACAGTA
    CAGTGTCTGC TGGACAGTGG TGCAGATATA AATAGGCCCA ATGTATCAGG TGCAACTCCA
    CTCTATTTTG CTTGCAGCCA TGGTCAGAGA GACACTGCAC AAATTCTTTT GATGAGAGGA
    GCCAAATACT TACCAGACAA AAATGGCATT ACTCCACTGG ATCTCTGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT CTTAATACAG TACCATCCTC GACTTTTCCA GACAATAATT
    CAAATGACTC AGAATGAAGA TCTACGGGAA AACATGTTGC GGCAAGTTCT GGAACATTTG
    TCTCAGCAGA GTGAAAGCCA GTATCTTAAA ATCCTGACAA GTCTTGCTGA AGTTGCTACA
    ACTAATGGTC ACAAACTACT TAGCTTGTCA AGTAATTATG AAGCTCAAAT GAAGAGTCTC
    TTAAGGATCG TGAGGATATT TTGCCATGTG TTTCGCATTG GCCCATCCTC TCCCAGTAAT
    GGAAATGACA TGGGCTACAA TGGAAATAAA ACTCCAAGAA GTCAGGTGTT CAAGCCACTA
    GAACTGCTGT GGCACTCATT AGACGAATGG CTAGTTCTTA TAGCCACAGA GCTGATGAAA
    AACAAAAGGG ACTCTGCCAA CATTACTTCT ATTTTGCTGA AACAAAAAGG ACCAGATCAC
    CAGGATGCTA CTCCTACGCC TTCCTTTGCC GCTGCAGGAA CAGAGAGCAG AAAAGAGCTA
    TCCACTGACA CAGGGGACTC AAAGACATAT GAAGTTGCGG GGAAGCAGGA AGCTTATGCT
    GATTGCCAGG ATGTTATCTC CATGACAGCA AACAGGCTAA GTGCTGTCAT TCAAGCATTT
    TATATGTGCT GCTCCTGTCA GATGCCTCAG GGGATGACGT CACCTCGTTT TATAGAATTT
    GTTTGTAAAC ACGATGATGT CCTTAAGTGT TTTGTTAACC GGAATCCCAA AATCATTTTT
    GATCACTTTC ATTTCCTTCT TGAGTGTCCT GAACTAATGT CCAGATTTAT GCACATCATA
    AAGGCTCAGC CATTTAAAGA TCGTTGTGAA TGGTTCTATG AACATCTGCA TTCAGGGCAA
    CCAGACTCAG ACATGGTGCA CAGACCTGTC AATGAAAATG ACATCCTTCT GGTTCACAGA
    GATTCTATTT TTAGGAGTAG CTGTGAAGTT GTCTCTAAAG CAAATTGTGC AAAACTAAAG
    CAAGGGATTG CTGTGCGATT TCACGGAGAA GAAGGCATGG GACAGGGTGT GGTGCGTGAG
    TGGTTTGATA TCTTATCCAG TGAAATAGTT AACCCAGATT ATGCCTTATT TACCCAGTCA
    GCTGATGGAA CAACCTTCCA GCCAAACAGC AACTCTTCTG TAAACCCAGA TCATTTGAAC
    TACTTCCGCT TTGCAGGCCA GATCCTGGGA TTAGCTCTCA ATCACAGGCA GCTGGTGAAC
    ATTTACTTCA CAAGATCATT CTACAAGCAT ATTCTTGGTA TACCTGTAAA TTATCAGGAT
    GTAGCATCTA TTGATCCAGA GTATGCAAAG AATTTGCAGT GGATTTTAGA TAATGATATC
    AGTGATCTGG GTTTAGAGCT GACTTTTTCT GTTGAGACTG ACGTATTTGG AGCAATGGAA
    GAAGTGCCAT TAAAGCCTGG GGGTGCGAGT ATACTTGTTA CACAGGAAAA CAAGGCTGAG
    TATGTCCAGC TTGTCACTGA ACTCAGAATG ACAAGAGCCA TTCAACCTCA GATAAATGCC
    TTTTTACAAG GCTTCCATAT GTTCATTCCA CCATCTTTGA TTCAACTTTT TGATGAATAT
    GAACTGGAGC TTTTGTTGTC TGGTATGCCG GAAATTGATG TGAATGATTG GTTAAAAAAT
    ACAGAATATA CCAGTGGCTA TGAGAGAGGA GACCAAGTAA TCCAGTGGTT CTGGGATGTT
    GTGGAAGAAT TAACTCAGGA AGAGAGAGTA CTACTATTAC AGTTTGTTAC TGGCAGTTCC
    AGAGTGCCTC ATGGTGGCTT TGCCCATATA ATGGGTGGGA GCGGATTGCA AAATTTTACA
    ATTGCTGCTG TGCCATATAC TGCGAACCTC TTACCAACAT CGAGCACGTG TATCAACATG
    CTCAAGTTAC CTGAATACCC AAGTAAAGAA ATCCTGAAGG ACAGGCTTCT TGTGGCATTG
    CACTGTGGAA GCTATGGTTA CACAATGGCA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33966
    Clone ID Related Accession (Same CDS sequence) XM_015284530.2 , XM_015284530.1
    Accession Version XM_015284530.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HACE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAGCAAGC TGCTGGAATA CAGTGCAGAT GTCAACATTT GTAATAACGA AGGCTTGACT 
    GCAATTCATT GGTTGGCTGT CAATGGACGG ACAGAATTGC TTCATGATCT TGTGCAGCAT
    GTCAGTAATG TAGATGTTGA AGATGCAATG GGACAGACAG CCCTTCATGT AGCTTGTCAA
    AATGGACACA AGACAACAGT ACAGTGTCTG CTGGACAGTG GTGCAGATAT AAATAGGCCC
    AATGTATCAG GTGCAACTCC ACTCTATTTT GCTTGCAGCC ATGGTCAGAG AGACACTGCA
    CAAATTCTTT TGATGAGAGG AGCCAAATAC TTACCAGACA AAAATGGCAT TACTCCACTG
    GATCTCTGTG TACAGGGTGG ATATGGAGAG ACTTGTGAAG TCTTAATACA GTACCATCCT
    CGACTTTTCC AGACAATAAT TCAAATGACT CAGAATGAAG ATCTACGGGA AAACATGTTG
    CGGCAAGTTC TGGAACATTT GTCTCAGCAG AGTGAAAGCC AGTATCTTAA AATCCTGACA
    AGTCTTGCTG AAGTTGCTAC AACTAATGGT CACAAACTAC TTAGCTTGTC AAGTAATTAT
    GAAGCTCAAA TGAAGAGTCT CTTAAGGATC GTGAGGATAT TTTGCCATGT GTTTCGCATT
    GGCCCATCCT CTCCCAGTAA TGGAAATGAC ATGGGCTACA ATGGAAATAA AACTCCAAGA
    AGTCAGGTGT TCAAGGTCAG AAAAGTATAT GATGTTGTTA GGAAGATAGA CGTTAAAGAG
    ATGAATTTCA CAAAGCATGC ATTCATTAAT CAGACATCTC ATGAACAGGA ACCACTAGAA
    CTGCTGTGGC ACTCATTAGA CGAATGGCTA GTTCTTATAG CCACAGAGCT GATGAAAAAC
    AAAAGGGACT CTGCCAACAT TACTTCTATT TTGCTGAAAC AAAAAGGACC AGATCACCAG
    GATGCTACTC CTACGCCTTC CTTTGCCGCT GCAGGAACAG AGAGCAGAAA AGAGCTATCC
    ACTGACACAG GGGACTCAAA GACATATGAA GTTGCGGGGA AGCAGGAAGC TTATGCTGAT
    TGCCAGGATG TTATCTCCAT GACAGCAAAC AGGCTAAGTG CTGTCATTCA AGCATTTTAT
    ATGTGCTGCT CCTGTCAGAT GCCTCAGGGG ATGACGTCAC CTCGTTTTAT AGAATTTGTT
    TGTAAACACG ATGATGTCCT TAAGTGTTTT GTTAACCGGA ATCCCAAAAT CATTTTTGAT
    CACTTTCATT TCCTTCTTGA GTGTCCTGAA CTAATGTCCA GATTTATGCA CATCATAAAG
    GCTCAGCCAT TTAAAGATCG TTGTGAATGG TTCTATGAAC ATCTGCATTC AGGGCAACCA
    GACTCAGACA TGGTGCACAG ACCTGTCAAT GAAAATGACA TCCTTCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTC TCTAAAGCAA ATTGTGCAAA ACTAAAGCAA
    GGGATTGCTG TGCGATTTCA CGGAGAAGAA GGCATGGGAC AGGGTGTGGT GCGTGAGTGG
    TTTGATATCT TATCCAGTGA AATAGTTAAC CCAGATTATG CCTTATTTAC CCAGTCAGCT
    GATGGAACAA CCTTCCAGCC AAACAGCAAC TCTTCTGTAA ACCCAGATCA TTTGAACTAC
    TTCCGCTTTG CAGGCCAGAT CCTGGGATTA GCTCTCAATC ACAGGCAGCT GGTGAACATT
    TACTTCACAA GATCATTCTA CAAGCATATT CTTGGTATAC CTGTAAATTA TCAGGATGTA
    GCATCTATTG ATCCAGAGTA TGCAAAGAAT TTGCAGTGGA TTTTAGATAA TGATATCAGT
    GATCTGGGTT TAGAGCTGAC TTTTTCTGTT GAGACTGACG TATTTGGAGC AATGGAAGAA
    GTGCCATTAA AGCCTGGGGG TGCGAGTATA CTTGTTACAC AGGAAAACAA GGCTGAGTAT
    GTCCAGCTTG TCACTGAACT CAGAATGACA AGAGCCATTC AACCTCAGAT AAATGCCTTT
    TTACAAGGCT TCCATATGTT CATTCCACCA TCTTTGATTC AACTTTTTGA TGAATATGAA
    CTGGAGCTTT TGTTGTCTGG TATGCCGGAA ATTGATGTGA ATGATTGGTT AAAAAATACA
    GAATATACCA GTGGCTATGA GAGAGGAGAC CAAGTAATCC AGTGGTTCTG GGATGTTGTG
    GAAGAATTAA CTCAGGAAGA GAGAGTACTA CTATTACAGT TTGTTACTGG CAGTTCCAGA
    GTGCCTCATG GTGGCTTTGC CCATATAATG GGTGGGAGCG GATTGCAAAA TTTTACAATT
    GCTGCTGTGC CATATACTGC GAACCTCTTA CCAACATCGA GCACGTGTAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATC CTGAAGGACA GGCTTCTTGT GGCATTGCAC
    TGTGGAAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140016.1
    CDS332..2821
    Translation

    Target ORF information:

    RefSeq Version XM_015284530.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284530.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAGCAAGC TGCTGGAATA CAGTGCAGAT GTCAACATTT GTAATAACGA AGGCTTGACT 
    GCAATTCATT GGTTGGCTGT CAATGGACGG ACAGAATTGC TTCATGATCT TGTGCAGCAT
    GTCAGTAATG TAGATGTTGA AGATGCAATG GGACAGACAG CCCTTCATGT AGCTTGTCAA
    AATGGACACA AGACAACAGT ACAGTGTCTG CTGGACAGTG GTGCAGATAT AAATAGGCCC
    AATGTATCAG GTGCAACTCC ACTCTATTTT GCTTGCAGCC ATGGTCAGAG AGACACTGCA
    CAAATTCTTT TGATGAGAGG AGCCAAATAC TTACCAGACA AAAATGGCAT TACTCCACTG
    GATCTCTGTG TACAGGGTGG ATATGGAGAG ACTTGTGAAG TCTTAATACA GTACCATCCT
    CGACTTTTCC AGACAATAAT TCAAATGACT CAGAATGAAG ATCTACGGGA AAACATGTTG
    CGGCAAGTTC TGGAACATTT GTCTCAGCAG AGTGAAAGCC AGTATCTTAA AATCCTGACA
    AGTCTTGCTG AAGTTGCTAC AACTAATGGT CACAAACTAC TTAGCTTGTC AAGTAATTAT
    GAAGCTCAAA TGAAGAGTCT CTTAAGGATC GTGAGGATAT TTTGCCATGT GTTTCGCATT
    GGCCCATCCT CTCCCAGTAA TGGAAATGAC ATGGGCTACA ATGGAAATAA AACTCCAAGA
    AGTCAGGTGT TCAAGGTCAG AAAAGTATAT GATGTTGTTA GGAAGATAGA CGTTAAAGAG
    ATGAATTTCA CAAAGCATGC ATTCATTAAT CAGACATCTC ATGAACAGGA ACCACTAGAA
    CTGCTGTGGC ACTCATTAGA CGAATGGCTA GTTCTTATAG CCACAGAGCT GATGAAAAAC
    AAAAGGGACT CTGCCAACAT TACTTCTATT TTGCTGAAAC AAAAAGGACC AGATCACCAG
    GATGCTACTC CTACGCCTTC CTTTGCCGCT GCAGGAACAG AGAGCAGAAA AGAGCTATCC
    ACTGACACAG GGGACTCAAA GACATATGAA GTTGCGGGGA AGCAGGAAGC TTATGCTGAT
    TGCCAGGATG TTATCTCCAT GACAGCAAAC AGGCTAAGTG CTGTCATTCA AGCATTTTAT
    ATGTGCTGCT CCTGTCAGAT GCCTCAGGGG ATGACGTCAC CTCGTTTTAT AGAATTTGTT
    TGTAAACACG ATGATGTCCT TAAGTGTTTT GTTAACCGGA ATCCCAAAAT CATTTTTGAT
    CACTTTCATT TCCTTCTTGA GTGTCCTGAA CTAATGTCCA GATTTATGCA CATCATAAAG
    GCTCAGCCAT TTAAAGATCG TTGTGAATGG TTCTATGAAC ATCTGCATTC AGGGCAACCA
    GACTCAGACA TGGTGCACAG ACCTGTCAAT GAAAATGACA TCCTTCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTC TCTAAAGCAA ATTGTGCAAA ACTAAAGCAA
    GGGATTGCTG TGCGATTTCA CGGAGAAGAA GGCATGGGAC AGGGTGTGGT GCGTGAGTGG
    TTTGATATCT TATCCAGTGA AATAGTTAAC CCAGATTATG CCTTATTTAC CCAGTCAGCT
    GATGGAACAA CCTTCCAGCC AAACAGCAAC TCTTCTGTAA ACCCAGATCA TTTGAACTAC
    TTCCGCTTTG CAGGCCAGAT CCTGGGATTA GCTCTCAATC ACAGGCAGCT GGTGAACATT
    TACTTCACAA GATCATTCTA CAAGCATATT CTTGGTATAC CTGTAAATTA TCAGGATGTA
    GCATCTATTG ATCCAGAGTA TGCAAAGAAT TTGCAGTGGA TTTTAGATAA TGATATCAGT
    GATCTGGGTT TAGAGCTGAC TTTTTCTGTT GAGACTGACG TATTTGGAGC AATGGAAGAA
    GTGCCATTAA AGCCTGGGGG TGCGAGTATA CTTGTTACAC AGGAAAACAA GGCTGAGTAT
    GTCCAGCTTG TCACTGAACT CAGAATGACA AGAGCCATTC AACCTCAGAT AAATGCCTTT
    TTACAAGGCT TCCATATGTT CATTCCACCA TCTTTGATTC AACTTTTTGA TGAATATGAA
    CTGGAGCTTT TGTTGTCTGG TATGCCGGAA ATTGATGTGA ATGATTGGTT AAAAAATACA
    GAATATACCA GTGGCTATGA GAGAGGAGAC CAAGTAATCC AGTGGTTCTG GGATGTTGTG
    GAAGAATTAA CTCAGGAAGA GAGAGTACTA CTATTACAGT TTGTTACTGG CAGTTCCAGA
    GTGCCTCATG GTGGCTTTGC CCATATAATG GGTGGGAGCG GATTGCAAAA TTTTACAATT
    GCTGCTGTGC CATATACTGC GAACCTCTTA CCAACATCGA GCACGTGTAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATC CTGAAGGACA GGCTTCTTGT GGCATTGCAC
    TGTGGAAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33966
    Clone ID Related Accession (Same CDS sequence) XM_015284530.2 , XM_015284530.1
    Accession Version XM_015284530.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HACE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455841.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAGCAAGC TGCTGGAATA CAGTGCAGAT GTCAACATTT GTAATAACGA AGGCTTGACT 
    GCAATTCATT GGTTGGCTGT CAATGGACGG ACAGAATTGC TTCATGATCT TGTGCAGCAT
    GTCAGTAATG TAGATGTTGA AGATGCAATG GGACAGACAG CCCTTCATGT AGCTTGTCAA
    AATGGACACA AGACAACAGT ACAGTGTCTG CTGGACAGTG GTGCAGATAT AAATAGGCCC
    AATGTATCAG GTGCAACTCC ACTCTATTTT GCTTGCAGCC ATGGTCAGAG AGACACTGCA
    CAAATTCTTT TGATGAGAGG AGCCAAATAC TTACCAGACA AAAATGGCAT TACTCCACTG
    GATCTCTGTG TACAGGGTGG ATATGGAGAG ACTTGTGAAG TCTTAATACA GTACCATCCT
    CGACTTTTCC AGACAATAAT TCAAATGACT CAGAATGAAG ATCTACGGGA AAACATGTTG
    CGGCAAGTTC TGGAACATTT GTCTCAGCAG AGTGAAAGCC AGTATCTTAA AATCCTGACA
    AGTCTTGCTG AAGTTGCTAC AACTAATGGT CACAAACTAC TTAGCTTGTC AAGTAATTAT
    GAAGCTCAAA TGAAGAGTCT CTTAAGGATC GTGAGGATAT TTTGCCATGT GTTTCGCATT
    GGCCCATCCT CTCCCAGTAA TGGAAATGAC ATGGGCTACA ATGGAAATAA AACTCCAAGA
    AGTCAGGTGT TCAAGGTCAG AAAAGTATAT GATGTTGTTA GGAAGATAGA CGTTAAAGAG
    ATGAATTTCA CAAAGCATGC ATTCATTAAT CAGACATCTC ATGAACAGGA ACCACTAGAA
    CTGCTGTGGC ACTCATTAGA CGAATGGCTA GTTCTTATAG CCACAGAGCT GATGAAAAAC
    AAAAGGGACT CTGCCAACAT TACTTCTATT TTGCTGAAAC AAAAAGGACC AGATCACCAG
    GATGCTACTC CTACGCCTTC CTTTGCCGCT GCAGGAACAG AGAGCAGAAA AGAGCTATCC
    ACTGACACAG GGGACTCAAA GACATATGAA GTTGCGGGGA AGCAGGAAGC TTATGCTGAT
    TGCCAGGATG TTATCTCCAT GACAGCAAAC AGGCTAAGTG CTGTCATTCA AGCATTTTAT
    ATGTGCTGCT CCTGTCAGAT GCCTCAGGGG ATGACGTCAC CTCGTTTTAT AGAATTTGTT
    TGTAAACACG ATGATGTCCT TAAGTGTTTT GTTAACCGGA ATCCCAAAAT CATTTTTGAT
    CACTTTCATT TCCTTCTTGA GTGTCCTGAA CTAATGTCCA GATTTATGCA CATCATAAAG
    GCTCAGCCAT TTAAAGATCG TTGTGAATGG TTCTATGAAC ATCTGCATTC AGGGCAACCA
    GACTCAGACA TGGTGCACAG ACCTGTCAAT GAAAATGACA TCCTTCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTC TCTAAAGCAA ATTGTGCAAA ACTAAAGCAA
    GGGATTGCTG TGCGATTTCA CGGAGAAGAA GGCATGGGAC AGGGTGTGGT GCGTGAGTGG
    TTTGATATCT TATCCAGTGA AATAGTTAAC CCAGATTATG CCTTATTTAC CCAGTCAGCT
    GATGGAACAA CCTTCCAGCC AAACAGCAAC TCTTCTGTAA ACCCAGATCA TTTGAACTAC
    TTCCGCTTTG CAGGCCAGAT CCTGGGATTA GCTCTCAATC ACAGGCAGCT GGTGAACATT
    TACTTCACAA GATCATTCTA CAAGCATATT CTTGGTATAC CTGTAAATTA TCAGGATGTA
    GCATCTATTG ATCCAGAGTA TGCAAAGAAT TTGCAGTGGA TTTTAGATAA TGATATCAGT
    GATCTGGGTT TAGAGCTGAC TTTTTCTGTT GAGACTGACG TATTTGGAGC AATGGAAGAA
    GTGCCATTAA AGCCTGGGGG TGCGAGTATA CTTGTTACAC AGGAAAACAA GGCTGAGTAT
    GTCCAGCTTG TCACTGAACT CAGAATGACA AGAGCCATTC AACCTCAGAT AAATGCCTTT
    TTACAAGGCT TCCATATGTT CATTCCACCA TCTTTGATTC AACTTTTTGA TGAATATGAA
    CTGGAGCTTT TGTTGTCTGG TATGCCGGAA ATTGATGTGA ATGATTGGTT AAAAAATACA
    GAATATACCA GTGGCTATGA GAGAGGAGAC CAAGTAATCC AGTGGTTCTG GGATGTTGTG
    GAAGAATTAA CTCAGGAAGA GAGAGTACTA CTATTACAGT TTGTTACTGG CAGTTCCAGA
    GTGCCTCATG GTGGCTTTGC CCATATAATG GGTGGGAGCG GATTGCAAAA TTTTACAATT
    GCTGCTGTGC CATATACTGC GAACCTCTTA CCAACATCGA GCACGTGTAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATC CTGAAGGACA GGCTTCTTGT GGCATTGCAC
    TGTGGAAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140016.1
    CDS332..2821
    Translation

    Target ORF information:

    RefSeq Version XM_015284530.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284530.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAGCAAGC TGCTGGAATA CAGTGCAGAT GTCAACATTT GTAATAACGA AGGCTTGACT 
    GCAATTCATT GGTTGGCTGT CAATGGACGG ACAGAATTGC TTCATGATCT TGTGCAGCAT
    GTCAGTAATG TAGATGTTGA AGATGCAATG GGACAGACAG CCCTTCATGT AGCTTGTCAA
    AATGGACACA AGACAACAGT ACAGTGTCTG CTGGACAGTG GTGCAGATAT AAATAGGCCC
    AATGTATCAG GTGCAACTCC ACTCTATTTT GCTTGCAGCC ATGGTCAGAG AGACACTGCA
    CAAATTCTTT TGATGAGAGG AGCCAAATAC TTACCAGACA AAAATGGCAT TACTCCACTG
    GATCTCTGTG TACAGGGTGG ATATGGAGAG ACTTGTGAAG TCTTAATACA GTACCATCCT
    CGACTTTTCC AGACAATAAT TCAAATGACT CAGAATGAAG ATCTACGGGA AAACATGTTG
    CGGCAAGTTC TGGAACATTT GTCTCAGCAG AGTGAAAGCC AGTATCTTAA AATCCTGACA
    AGTCTTGCTG AAGTTGCTAC AACTAATGGT CACAAACTAC TTAGCTTGTC AAGTAATTAT
    GAAGCTCAAA TGAAGAGTCT CTTAAGGATC GTGAGGATAT TTTGCCATGT GTTTCGCATT
    GGCCCATCCT CTCCCAGTAA TGGAAATGAC ATGGGCTACA ATGGAAATAA AACTCCAAGA
    AGTCAGGTGT TCAAGGTCAG AAAAGTATAT GATGTTGTTA GGAAGATAGA CGTTAAAGAG
    ATGAATTTCA CAAAGCATGC ATTCATTAAT CAGACATCTC ATGAACAGGA ACCACTAGAA
    CTGCTGTGGC ACTCATTAGA CGAATGGCTA GTTCTTATAG CCACAGAGCT GATGAAAAAC
    AAAAGGGACT CTGCCAACAT TACTTCTATT TTGCTGAAAC AAAAAGGACC AGATCACCAG
    GATGCTACTC CTACGCCTTC CTTTGCCGCT GCAGGAACAG AGAGCAGAAA AGAGCTATCC
    ACTGACACAG GGGACTCAAA GACATATGAA GTTGCGGGGA AGCAGGAAGC TTATGCTGAT
    TGCCAGGATG TTATCTCCAT GACAGCAAAC AGGCTAAGTG CTGTCATTCA AGCATTTTAT
    ATGTGCTGCT CCTGTCAGAT GCCTCAGGGG ATGACGTCAC CTCGTTTTAT AGAATTTGTT
    TGTAAACACG ATGATGTCCT TAAGTGTTTT GTTAACCGGA ATCCCAAAAT CATTTTTGAT
    CACTTTCATT TCCTTCTTGA GTGTCCTGAA CTAATGTCCA GATTTATGCA CATCATAAAG
    GCTCAGCCAT TTAAAGATCG TTGTGAATGG TTCTATGAAC ATCTGCATTC AGGGCAACCA
    GACTCAGACA TGGTGCACAG ACCTGTCAAT GAAAATGACA TCCTTCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTC TCTAAAGCAA ATTGTGCAAA ACTAAAGCAA
    GGGATTGCTG TGCGATTTCA CGGAGAAGAA GGCATGGGAC AGGGTGTGGT GCGTGAGTGG
    TTTGATATCT TATCCAGTGA AATAGTTAAC CCAGATTATG CCTTATTTAC CCAGTCAGCT
    GATGGAACAA CCTTCCAGCC AAACAGCAAC TCTTCTGTAA ACCCAGATCA TTTGAACTAC
    TTCCGCTTTG CAGGCCAGAT CCTGGGATTA GCTCTCAATC ACAGGCAGCT GGTGAACATT
    TACTTCACAA GATCATTCTA CAAGCATATT CTTGGTATAC CTGTAAATTA TCAGGATGTA
    GCATCTATTG ATCCAGAGTA TGCAAAGAAT TTGCAGTGGA TTTTAGATAA TGATATCAGT
    GATCTGGGTT TAGAGCTGAC TTTTTCTGTT GAGACTGACG TATTTGGAGC AATGGAAGAA
    GTGCCATTAA AGCCTGGGGG TGCGAGTATA CTTGTTACAC AGGAAAACAA GGCTGAGTAT
    GTCCAGCTTG TCACTGAACT CAGAATGACA AGAGCCATTC AACCTCAGAT AAATGCCTTT
    TTACAAGGCT TCCATATGTT CATTCCACCA TCTTTGATTC AACTTTTTGA TGAATATGAA
    CTGGAGCTTT TGTTGTCTGG TATGCCGGAA ATTGATGTGA ATGATTGGTT AAAAAATACA
    GAATATACCA GTGGCTATGA GAGAGGAGAC CAAGTAATCC AGTGGTTCTG GGATGTTGTG
    GAAGAATTAA CTCAGGAAGA GAGAGTACTA CTATTACAGT TTGTTACTGG CAGTTCCAGA
    GTGCCTCATG GTGGCTTTGC CCATATAATG GGTGGGAGCG GATTGCAAAA TTTTACAATT
    GCTGCTGTGC CATATACTGC GAACCTCTTA CCAACATCGA GCACGTGTAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATC CTGAAGGACA GGCTTCTTGT GGCATTGCAC
    TGTGGAAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33967
    Clone ID Related Accession (Same CDS sequence) XM_015284531.1
    Accession Version XM_015284531.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HACE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455841.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAGAGAG CCATGGAGCA GCTCAACCGC CTCACCAGGT CCTTGCGCCG CGCCCGCACC 
    GTGGAGCTGC CGGACGATAA CGAAACTGCT GTTTATACAC TAATGCCAAT GGTGATGGCT
    GACCAGCACA GGTCTGTCTC AGAGCTGCTA TCAAATTCAA AATTTGATGT GAATTATGCA
    TTTGGACGTG TGAAGAGAAG TTTGCTTCAT ATTGCAGCAA ACTGTGGCTC AGTTGAATGC
    CTTGTTCTGT TATTAAAAAA AGGAGCAAAT CCAAACTATC AGGACATCTC AGGATGCACA
    CCTCTCCATT TAGCAGCAAG GAATGGCCAG AAAAAATGCA TGAGCAAGCT GCTGGAATAC
    AGTGCAGATG TCAACATTTG TAATAACGAA GGCTTGACTG CAATTCATTG GTTGGCTGTC
    AATGGACGGA CAGAATTGCT TCATGATCTT GTGCAGCATG TCAGTAATGT AGATGTTGAA
    GATGCAATGG GACAGACAGC CCTTCATGTA GCTTGTCAAA ATGGACACAA GACAACAGTA
    CAGTGTCTGC TGGACAGTGG TGCAGATATA AATAGGCCCA ATGTATCAGG TGCAACTCCA
    CTCTATTTTG CTTGCAGCCA TGGTCAGAGA GACACTGCAC AAATTCTTTT GATGAGAGGA
    GCCAAATACT TACCAGACAA AAATGGCATT ACTCCACTGG ATCTCTGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT CTTAATACAG TACCATCCTC GACTTTTCCA GACAATAATT
    CAAATGACTC AGAATGAAGA TCTACGGGAA AACATGTTGC GGCAAGTTCT GGAACATTTG
    TCTCAGCAGA GTGAAAGCCA GTATCTTAAA ATCCTGACAA GTCTTGCTGA AGTTGCTACA
    ACTAATGGTC ACAAACTACT TAGCTTGTCA AGTAATTATG AAGCTCAAAT GAAGAGTCTC
    TTAAGGATCG TGAGGATATT TTGCCATGTG TTTCGCATTG GCCCATCCTC TCCCAGTAAT
    GGAAATGACA TGGGCTACAA TGGAAATAAA ACTCCAAGAA GTCAGGTGTT CAAGGTCAGA
    AAAGTATATG ATGTTGTTAG GAAGATAGAC GTTAAAGAGA TGAATTTCAC AAAGCATGCA
    TTCATTAATC AGACATCTCA TGAACAGGAA CCACTAGAAC TGCTGTGGCA CTCATTAGAC
    GAATGGCTAG TTCTTATAGC CACAGAGCTG ATGAAAAACA AAAGGGACTC TGCCAACATT
    ACTTCTATTT TGCTGAAACA AAAAGGACCA GATCACCAGG ATGCTACTCC TACGCCTTCC
    TTTGCCGCTG CAGGAACAGA GAGCAGAAAA GAGCTATCCA CTGACACAGG GGACTCAAAG
    ACATATGAAG TTGCGGGGAA GCAGGAAGCT TATGCTGATT GCCAGGATGT TATCTCCATG
    ACAGCAAACA GGCTAAGTGC TGTCATTCAA GCATTTTATA TGTGCTGCTC CTGTCAGATG
    CCTCAGGGGA TGACGTCACC TCGTTTTATA GAATTTGTTT GTAAACACGA TGATGTCCTT
    AAGTGTTTTG TTAACCGGAA TCCCAAAATC ATTTTTGATC ACTTTCATTT CCTTCTTGAG
    TGTCCTGAAC TAATGTCCAG ATTTATGCAC ATCATAAAGG CTCAGCCATT TAAAGATCGT
    TGTGAATGGT TCTATGAACA TCTGCATTCA GGGCAACCAG ACTCAGACAT GGTGCACAGA
    CCTGTCAATG AAAATGACAT CCTTCTGGTT CACAGAGATT CTATTTTTAG GAGTAGCTGT
    GAAGTTGTCT CTAAAGCAAA TTGTGCAAAA CTAAAGCAAG GGATTGCTGT GCGATTTCAC
    GGAGAAGAAG GCATGGGACA GGGTGTGGTG CGTGAGTGGT TTGATATCTT ATCCAGTGAA
    ATAGTTAACC CAGATTATGC CTTATTTACC CAGTCAGCTG ATGGAACAAC CTTCCAGCCA
    AACAGCAACT CTTCTGTAAA CCCAGATCAT TTGAACTACT TCCGCTTTGC AGGCCAGATC
    CTGGGATTAG CTCTCAATCA CAGGCAGCTG GTGAACATTT ACTTCACAAG ATCATTCTAC
    AAGCATATTC TTGGTATACC TGTAAATTAT CAGGATGTAG CATCTATTGA TCCAGAGTAT
    GCAAAGAATT TGCAGTGGAT TTTAGATAAT GATATCAGTG ATCTGGGTTT AGAGCTGACT
    TTTTCTGTTG AGACTGACGT ATTTGGAGCA ATGGAAGAAG TGCCATTAAA GCCTGGGGGT
    GCGAGTATAC TTGTTACACA GGAAAACAAG GTCCATCTTG ACTGGCTGCT GCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140017.1
    CDS96..2432
    Translation

    Target ORF information:

    RefSeq Version XM_015284531.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAGAGAG CCATGGAGCA GCTCAACCGC CTCACCAGGT CCTTGCGCCG CGCCCGCACC 
    GTGGAGCTGC CGGACGATAA CGAAACTGCT GTTTATACAC TAATGCCAAT GGTGATGGCT
    GACCAGCACA GGTCTGTCTC AGAGCTGCTA TCAAATTCAA AATTTGATGT GAATTATGCA
    TTTGGACGTG TGAAGAGAAG TTTGCTTCAT ATTGCAGCAA ACTGTGGCTC AGTTGAATGC
    CTTGTTCTGT TATTAAAAAA AGGAGCAAAT CCAAACTATC AGGACATCTC AGGATGCACA
    CCTCTCCATT TAGCAGCAAG GAATGGCCAG AAAAAATGCA TGAGCAAGCT GCTGGAATAC
    AGTGCAGATG TCAACATTTG TAATAACGAA GGCTTGACTG CAATTCATTG GTTGGCTGTC
    AATGGACGGA CAGAATTGCT TCATGATCTT GTGCAGCATG TCAGTAATGT AGATGTTGAA
    GATGCAATGG GACAGACAGC CCTTCATGTA GCTTGTCAAA ATGGACACAA GACAACAGTA
    CAGTGTCTGC TGGACAGTGG TGCAGATATA AATAGGCCCA ATGTATCAGG TGCAACTCCA
    CTCTATTTTG CTTGCAGCCA TGGTCAGAGA GACACTGCAC AAATTCTTTT GATGAGAGGA
    GCCAAATACT TACCAGACAA AAATGGCATT ACTCCACTGG ATCTCTGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT CTTAATACAG TACCATCCTC GACTTTTCCA GACAATAATT
    CAAATGACTC AGAATGAAGA TCTACGGGAA AACATGTTGC GGCAAGTTCT GGAACATTTG
    TCTCAGCAGA GTGAAAGCCA GTATCTTAAA ATCCTGACAA GTCTTGCTGA AGTTGCTACA
    ACTAATGGTC ACAAACTACT TAGCTTGTCA AGTAATTATG AAGCTCAAAT GAAGAGTCTC
    TTAAGGATCG TGAGGATATT TTGCCATGTG TTTCGCATTG GCCCATCCTC TCCCAGTAAT
    GGAAATGACA TGGGCTACAA TGGAAATAAA ACTCCAAGAA GTCAGGTGTT CAAGGTCAGA
    AAAGTATATG ATGTTGTTAG GAAGATAGAC GTTAAAGAGA TGAATTTCAC AAAGCATGCA
    TTCATTAATC AGACATCTCA TGAACAGGAA CCACTAGAAC TGCTGTGGCA CTCATTAGAC
    GAATGGCTAG TTCTTATAGC CACAGAGCTG ATGAAAAACA AAAGGGACTC TGCCAACATT
    ACTTCTATTT TGCTGAAACA AAAAGGACCA GATCACCAGG ATGCTACTCC TACGCCTTCC
    TTTGCCGCTG CAGGAACAGA GAGCAGAAAA GAGCTATCCA CTGACACAGG GGACTCAAAG
    ACATATGAAG TTGCGGGGAA GCAGGAAGCT TATGCTGATT GCCAGGATGT TATCTCCATG
    ACAGCAAACA GGCTAAGTGC TGTCATTCAA GCATTTTATA TGTGCTGCTC CTGTCAGATG
    CCTCAGGGGA TGACGTCACC TCGTTTTATA GAATTTGTTT GTAAACACGA TGATGTCCTT
    AAGTGTTTTG TTAACCGGAA TCCCAAAATC ATTTTTGATC ACTTTCATTT CCTTCTTGAG
    TGTCCTGAAC TAATGTCCAG ATTTATGCAC ATCATAAAGG CTCAGCCATT TAAAGATCGT
    TGTGAATGGT TCTATGAACA TCTGCATTCA GGGCAACCAG ACTCAGACAT GGTGCACAGA
    CCTGTCAATG AAAATGACAT CCTTCTGGTT CACAGAGATT CTATTTTTAG GAGTAGCTGT
    GAAGTTGTCT CTAAAGCAAA TTGTGCAAAA CTAAAGCAAG GGATTGCTGT GCGATTTCAC
    GGAGAAGAAG GCATGGGACA GGGTGTGGTG CGTGAGTGGT TTGATATCTT ATCCAGTGAA
    ATAGTTAACC CAGATTATGC CTTATTTACC CAGTCAGCTG ATGGAACAAC CTTCCAGCCA
    AACAGCAACT CTTCTGTAAA CCCAGATCAT TTGAACTACT TCCGCTTTGC AGGCCAGATC
    CTGGGATTAG CTCTCAATCA CAGGCAGCTG GTGAACATTT ACTTCACAAG ATCATTCTAC
    AAGCATATTC TTGGTATACC TGTAAATTAT CAGGATGTAG CATCTATTGA TCCAGAGTAT
    GCAAAGAATT TGCAGTGGAT TTTAGATAAT GATATCAGTG ATCTGGGTTT AGAGCTGACT
    TTTTCTGTTG AGACTGACGT ATTTGGAGCA ATGGAAGAAG TGCCATTAAA GCCTGGGGGT
    GCGAGTATAC TTGTTACACA GGAAAACAAG GTCCATCTTG ACTGGCTGCT GCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.