HACE1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005210843.3	XP_005210900.1	E3 ubiquitin-protein ligase HACE1 isoform X4
NM_001192645.2	NP_001179574.2	E3 ubiquitin-protein ligase HACE1
XM_005210841.3	XP_005210898.1	E3 ubiquitin-protein ligase HACE1 isoform X1
XM_005210842.3	XP_005210899.1	E3 ubiquitin-protein ligase HACE1 isoform X2
XM_024996667.1	XP_024852435.1	E3 ubiquitin-protein ligase HACE1 isoform X1
XM_024996669.1	XP_024852437.1	E3 ubiquitin-protein ligase HACE1 isoform X3
XM_024996670.1	XP_024852438.1	E3 ubiquitin-protein ligase HACE1 isoform X4
XM_024996672.1	XP_024852440.1	E3 ubiquitin-protein ligase HACE1 isoform X8
XM_010808467.2	XP_010806769.1	E3 ubiquitin-protein ligase HACE1 isoform X3
XM_005210843.4	XP_005210900.1	E3 ubiquitin-protein ligase HACE1 isoform X6
XM_024996668.1	XP_024852436.1	E3 ubiquitin-protein ligase HACE1 isoform X2
XM_010808467.3	XP_010806769.1	E3 ubiquitin-protein ligase HACE1 isoform X5
XM_024996671.1	XP_024852439.1	E3 ubiquitin-protein ligase HACE1 isoform X7
XM_024996673.1	XP_024852441.1	E3 ubiquitin-protein ligase HACE1 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa00714	NM_001192645.2 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa22599	XM_005210841.3 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa22600	XM_005210842.3 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa22601	XM_005210843.4 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa51409	XM_010808467.3 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa237783	XM_024996667.1 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa237784	XM_024996668.1 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa237785	XM_024996669.1 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa237786	XM_024996670.1 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa237787	XM_024996671.1 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa237788	XM_024996672.1 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa237789	XM_024996673.1 Latest version!	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OBa22601	XM_005210843.3	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa51409	XM_010808467.2	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

HACE1 ( NM_001192645.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179574.2 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), mRNA.

Price & Availability↑

CloneID	OBa00714
Clone ID Related Accession (Same CDS sequence)	NM_001192645.2
Accession Version	NM_001192645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH179641.1 and NKLS02000009.1. On Apr 22, 2011 this sequence version replaced NM_001192645.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571263.4530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG 
GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA 
CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT 
GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC 
CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG 
GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT 
TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT 
GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC 
GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG 
GTCCCACATG GTGGATTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTCACAATT 
GCTGCTGTGC CATATACTCC AAATCTTCTA CCTACTTCAA GCACATGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG CAAAGAAATA CTCAAGGACA GGCTTCTTGT GGCACTACAT 
TGTGGCAGCT ATGGTTACAC AATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179574.2
CDS	180..2909
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001192645.2
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192645.2

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG 
GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA 
CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT 
GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC 
CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG 
GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT 
TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT 
GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC 
GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG 
GTCCCACATG GTGGATTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTCACAATT 
GCTGCTGTGC CATATACTCC AAATCTTCTA CCTACTTCAA GCACATGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG CAAAGAAATA CTCAAGGACA GGCTTCTTGT GGCACTACAT 
TGTGGCAGCT ATGGTTACAC AATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_005210841.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210898.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa22599
Clone ID Related Accession (Same CDS sequence)	XM_005210841.3
Accession Version	XM_005210841.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104193.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210841.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GTTGTGGATC AGTGGAATGC TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT 
CCTAACTATC AAGATATTTC GGGCTGTACA CCTCTTCATT TGGCAGCTAG AAATGGGCAA 
AAGAAATGCA TGAGTAAATT ATTAGAATAC AGTGCTGATG TCAACATTTG TAATAATGAA 
GGTCTTACTG CAATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT 
GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG 
GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT 
AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA 
GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA 
ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG 
TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA 
AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG 
ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT 
AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC 
TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA 
ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG 
CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT 
ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT 
CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG 
CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC 
CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA 
ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT 
GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA 
TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG 
TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT 
GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG 
TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA 
GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT 
CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC 
TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA 
GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT 
CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC 
TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT 
GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT 
CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA 
AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA 
TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA 
ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT 
CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT 
CTCTTGCAGT TTGTTACTGG CAGTTCCAGG GTCCCACATG GTGGATTTGC TAATATCATG 
GGTGGAAGTG GATTGCAAAA CTTCACAATT GCTGCTGTGC CATATACTCC AAATCTTCTA 
CCTACTTCAA GCACATGCAT CAACATGCTC AAGTTACCTG AATACCCAAG CAAAGAAATA 
CTCAAGGACA GGCTTCTTGT GGCACTACAT TGTGGCAGCT ATGGTTACAC AATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210898.1
CDS	240..2879
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHSCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_005210841.3
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210841.3

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GTTGTGGATC AGTGGAATGC TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT 
CCTAACTATC AAGATATTTC GGGCTGTACA CCTCTTCATT TGGCAGCTAG AAATGGGCAA 
AAGAAATGCA TGAGTAAATT ATTAGAATAC AGTGCTGATG TCAACATTTG TAATAATGAA 
GGTCTTACTG CAATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT 
GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG 
GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT 
AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA 
GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA 
ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG 
TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA 
AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG 
ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT 
AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC 
TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA 
ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG 
CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT 
ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT 
CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG 
CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC 
CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA 
ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT 
GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA 
TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG 
TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT 
GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG 
TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA 
GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT 
CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC 
TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA 
GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT 
CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC 
TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT 
GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT 
CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA 
AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA 
TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA 
ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT 
CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT 
CTCTTGCAGT TTGTTACTGG CAGTTCCAGG GTCCCACATG GTGGATTTGC TAATATCATG 
GGTGGAAGTG GATTGCAAAA CTTCACAATT GCTGCTGTGC CATATACTCC AAATCTTCTA 
CCTACTTCAA GCACATGCAT CAACATGCTC AAGTTACCTG AATACCCAAG CAAAGAAATA 
CTCAAGGACA GGCTTCTTGT GGCACTACAT TGTGGCAGCT ATGGTTACAC AATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_005210842.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210899.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa22600
Clone ID Related Accession (Same CDS sequence)	XM_005210842.3
Accession Version	XM_005210842.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104193.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210842.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACC CTTCAGCACC AGTTTATAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCAG TGGAATGCTT GGTTCTGCTT TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCGG GCTGTACACC TCTTCATTTG GCAGCTAGAA ATGGGCAAAA GAAATGCATG 
AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG TCTTACTGCA 
ATACACTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT ACAACACGTC 
AGTGATGTCG ATGTTGAGGA TGCGATGGGG CAGACAGCGC TGCATGTGGC CTGCCAGAAT 
GGTCACAAGA CGACAGTGCA ATGCTTGCTG GACAGTGGTG CTGATATTAA CAGACCAAAT 
GTATCAGGAG CCACTCCACT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAG 
ATTCTTCTAT TACGAGGTGC CAAGTATCTG TCAGATAAAA ACGGAGTAAC CCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACC TGTGAAGTAT TAATTCAGTA TCACCCTAGG 
CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ATTTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCCACAAC AAATGGTCAT AAATTGCTTA GCATATCTAG TAATTACGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT 
CCATCCTCTC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGGAGC 
CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA 
GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTGCTGAAA 
CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAGGG GAATCTAAAC CAGATGTGCT TGGAGGGAAA 
CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACTTCGCCC 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAACAGGAAT 
CCCAAGATTA TATTTGACCA CTTTCACTTC CTTCTCGAAT GTCCTGAATT GATGTCAAGA 
TTCATGCATA TCATAAAGGC ACAGCCATTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGACATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ATAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTCC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAACCCA ATAGCAACTC CTCTGTAAAT 
CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TCTGAACCAT 
AGGCAACTGG TGAACATTTA CTTCACGAGA TCATTCTATA AGCACATTCT TGGTATTCCT 
GTGAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAACTT GCAATGGATT 
TTAGATAATG ATATTAGTGA TTTGGGTCTA GAATTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA CCTGGGGGTG GAAGTATTCT TGTAACACAA 
AATAACAAAG CGGAGTACGT CCAGCTTGTC ACTGAACTGC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCATC CCTCATACAA 
CTTTTTGATG AATACGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTGGA AGATATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT 
GTTACTGGCA GTTCCAGGGT CCCACATGGT GGATTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TCACAATTGC TGCTGTGCCA TATACTCCAA ATCTTCTACC TACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGCA AAGAAATACT CAAGGACAGG 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210899.1
CDS	149..2776
Translation	MRNPSAPVYRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_005210842.3
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210842.3

ORF Insert Sequence:

ATGAGGAACC CTTCAGCACC AGTTTATAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCAG TGGAATGCTT GGTTCTGCTT TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCGG GCTGTACACC TCTTCATTTG GCAGCTAGAA ATGGGCAAAA GAAATGCATG 
AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG TCTTACTGCA 
ATACACTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT ACAACACGTC 
AGTGATGTCG ATGTTGAGGA TGCGATGGGG CAGACAGCGC TGCATGTGGC CTGCCAGAAT 
GGTCACAAGA CGACAGTGCA ATGCTTGCTG GACAGTGGTG CTGATATTAA CAGACCAAAT 
GTATCAGGAG CCACTCCACT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAG 
ATTCTTCTAT TACGAGGTGC CAAGTATCTG TCAGATAAAA ACGGAGTAAC CCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACC TGTGAAGTAT TAATTCAGTA TCACCCTAGG 
CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ATTTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCCACAAC AAATGGTCAT AAATTGCTTA GCATATCTAG TAATTACGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT 
CCATCCTCTC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGGAGC 
CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA 
GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTGCTGAAA 
CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAGGG GAATCTAAAC CAGATGTGCT TGGAGGGAAA 
CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACTTCGCCC 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAACAGGAAT 
CCCAAGATTA TATTTGACCA CTTTCACTTC CTTCTCGAAT GTCCTGAATT GATGTCAAGA 
TTCATGCATA TCATAAAGGC ACAGCCATTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGACATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ATAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTCC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAACCCA ATAGCAACTC CTCTGTAAAT 
CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TCTGAACCAT 
AGGCAACTGG TGAACATTTA CTTCACGAGA TCATTCTATA AGCACATTCT TGGTATTCCT 
GTGAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAACTT GCAATGGATT 
TTAGATAATG ATATTAGTGA TTTGGGTCTA GAATTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA CCTGGGGGTG GAAGTATTCT TGTAACACAA 
AATAACAAAG CGGAGTACGT CCAGCTTGTC ACTGAACTGC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCATC CCTCATACAA 
CTTTTTGATG AATACGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTGGA AGATATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT 
GTTACTGGCA GTTCCAGGGT CCCACATGGT GGATTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TCACAATTGC TGCTGTGCCA TATACTCCAA ATCTTCTACC TACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGCA AAGAAATACT CAAGGACAGG 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_005210843.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210900.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa22601
Clone ID Related Accession (Same CDS sequence)	XM_005210843.3 , XM_005210843.4
Accession Version	XM_005210843.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104193.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG 
GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG 
TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC 
TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG 
AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT 
GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT 
AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG 
AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG 
GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG 
CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC 
TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG 
GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC 
CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT 
AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG 
TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT 
TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT 
GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA 
TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA 
GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA 
CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC 
ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT 
CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT 
GGATTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TCACAATTGC TGCTGTGCCA 
TATACTCCAA ATCTTCTACC TACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGCA AAGAAATACT CAAGGACAGG CTTCTTGTGG CACTACATTG TGGCAGCTAT 
GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210900.1
CDS	240..2837
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTATVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_005210843.3
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210843.3

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG 
GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG 
TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC 
TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG 
AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT 
GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT 
AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG 
AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG 
GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG 
CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC 
TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG 
GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC 
CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT 
AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG 
TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT 
TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT 
GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA 
TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA 
GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA 
CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC 
ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT 
CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT 
GGATTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TCACAATTGC TGCTGTGCCA 
TATACTCCAA ATCTTCTACC TACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGCA AAGAAATACT CAAGGACAGG CTTCTTGTGG CACTACATTG TGGCAGCTAT 
GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_005210843.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210900.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X6, mRNA.

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CloneID	OBa22601
Clone ID Related Accession (Same CDS sequence)	XM_005210843.3 , XM_005210843.4
Accession Version	XM_005210843.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210843.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG 
GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG 
TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC 
TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG 
AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT 
GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT 
AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG 
AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG 
GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG 
CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC 
TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG 
GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC 
CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT 
AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG 
TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT 
TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT 
GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA 
TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA 
GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA 
CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC 
ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT 
CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT 
GGATTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TCACAATTGC TGCTGTGCCA 
TATACTCCAA ATCTTCTACC TACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGCA AAGAAATACT CAAGGACAGG CTTCTTGTGG CACTACATTG TGGCAGCTAT 
GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210900.1
CDS	239..2836
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTATVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_005210843.4
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210843.4

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG 
GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG 
TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC 
TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG 
AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT 
GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT 
AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG 
AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG 
GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG 
CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC 
TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG 
GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC 
CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT 
AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG 
TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT 
TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT 
GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA 
TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA 
GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA 
CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC 
ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT 
CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT 
GGATTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TCACAATTGC TGCTGTGCCA 
TATACTCCAA ATCTTCTACC TACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGCA AAGAAATACT CAAGGACAGG CTTCTTGTGG CACTACATTG TGGCAGCTAT 
GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_010808467.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806769.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa51409
Clone ID Related Accession (Same CDS sequence)	XM_010808467.2 , XM_010808467.3
Accession Version	XM_010808467.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104193.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808467.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG 
GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA 
CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT 
GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC 
CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG 
GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT 
TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT 
GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC 
GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806769.1
CDS	242..2857
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_010808467.2
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808467.2

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG 
GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA 
CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT 
GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC 
CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG 
GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT 
TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT 
GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC 
GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_010808467.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806769.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa51409
Clone ID Related Accession (Same CDS sequence)	XM_010808467.2 , XM_010808467.3
Accession Version	XM_010808467.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010808467.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG 
GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA 
CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT 
GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC 
CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG 
GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT 
TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT 
GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC 
GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806769.1
CDS	239..2854
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_010808467.3
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808467.3

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG 
GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA 
CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT 
GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC 
CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG 
GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT 
TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT 
GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC 
GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_024996667.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852435.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa237783
Clone ID Related Accession (Same CDS sequence)	XM_024996667.1
Accession Version	XM_024996667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG 
GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA 
CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT 
GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC 
CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG 
GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT 
TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT 
GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC 
GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCCGTGAA 
GAGATACCTC ACGTCCAAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAT ATCATGGGTG 
GAAGTGGATT GCAAAACTTC ACAATTGCTG CTGTGCCATA TACTCCAAAT CTTCTACCTA 
CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA 
AGGACAGGCT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG GCATAATGAA 
GTCTGGAAAA TTCTTCTGAC TGCTGATGAG CAATTCAGAG TGGCAGAAGT AATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852435.1
CDS	251..3067
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSREEIPHVQVPGSHMVDLLISWVEVDCKTSQLLLCHILQIFYLLQAHASTCSSYLNTQAKKYSRTGFLWHYIVAAMVTQWHNEVWKILLTADEQFRVAEVI

Target ORF information:

RefSeq Version	XM_024996667.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996667.1

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG 
GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA 
CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT 
GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC 
CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG 
GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT 
TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT 
GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC 
GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCCGTGAA 
GAGATACCTC ACGTCCAAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAT ATCATGGGTG 
GAAGTGGATT GCAAAACTTC ACAATTGCTG CTGTGCCATA TACTCCAAAT CTTCTACCTA 
CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA 
AGGACAGGCT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG GCATAATGAA 
GTCTGGAAAA TTCTTCTGAC TGCTGATGAG CAATTCAGAG TGGCAGAAGT AATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_024996668.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852436.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa237784
Clone ID Related Accession (Same CDS sequence)	XM_024996668.1
Accession Version	XM_024996668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GTTGTGGATC AGTGGAATGC TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT 
CCTAACTATC AAGATATTTC GGGCTGTACA CCTCTTCATT TGGCAGCTAG AAATGGGCAA 
AAGAAATGCA TGAGTAAATT ATTAGAATAC AGTGCTGATG TCAACATTTG TAATAATGAA 
GGTCTTACTG CAATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT 
GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG 
GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT 
AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA 
GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA 
ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG 
TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA 
AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG 
ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT 
AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC 
TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA 
ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG 
CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT 
ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT 
CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG 
CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC 
CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA 
ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT 
GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA 
TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG 
TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT 
GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG 
TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA 
GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT 
CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC 
TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA 
GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT 
CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC 
TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT 
GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT 
CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA 
AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA 
TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA 
ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT 
CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT 
CTCTTGCAGT TTGTTACTGG CAGCCGTGAA GAGATACCTC ACGTCCAAGT TCCAGGGTCC 
CACATGGTGG ATTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG 
CTGTGCCATA TACTCCAAAT CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT 
TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG 
GCAGCTATGG TTACACAATG GCATAATGAA GTCTGGAAAA TTCTTCTGAC TGCTGATGAG 
CAATTCAGAG TGGCAGAAGT AATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852436.1
CDS	251..2977
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHSCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSREEIPHVQVPGSHMVDLLISWVEVDCKTSQLLLCHILQIFYLLQAHASTCSSYLNTQAKKYSRTGFLWHYIVAAMVTQWHNEVWKILLTADEQFRVAEVI

Target ORF information:

RefSeq Version	XM_024996668.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996668.1

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GTTGTGGATC AGTGGAATGC TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT 
CCTAACTATC AAGATATTTC GGGCTGTACA CCTCTTCATT TGGCAGCTAG AAATGGGCAA 
AAGAAATGCA TGAGTAAATT ATTAGAATAC AGTGCTGATG TCAACATTTG TAATAATGAA 
GGTCTTACTG CAATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT 
GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG 
GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT 
AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA 
GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA 
ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG 
TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA 
AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG 
ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT 
AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC 
TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA 
ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG 
CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT 
ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT 
CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG 
CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC 
CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA 
ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT 
GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA 
TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG 
TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT 
GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG 
TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA 
GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT 
CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC 
TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA 
GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT 
CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC 
TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT 
GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT 
CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA 
AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA 
TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA 
ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT 
CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT 
CTCTTGCAGT TTGTTACTGG CAGCCGTGAA GAGATACCTC ACGTCCAAGT TCCAGGGTCC 
CACATGGTGG ATTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG 
CTGTGCCATA TACTCCAAAT CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT 
TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG 
GCAGCTATGG TTACACAATG GCATAATGAA GTCTGGAAAA TTCTTCTGAC TGCTGATGAG 
CAATTCAGAG TGGCAGAAGT AATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_024996669.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852437.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa237785
Clone ID Related Accession (Same CDS sequence)	XM_024996669.1
Accession Version	XM_024996669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACC CTTCAGCACC AGTTTATAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCAG TGGAATGCTT GGTTCTGCTT TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCGG GCTGTACACC TCTTCATTTG GCAGCTAGAA ATGGGCAAAA GAAATGCATG 
AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG TCTTACTGCA 
ATACACTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT ACAACACGTC 
AGTGATGTCG ATGTTGAGGA TGCGATGGGG CAGACAGCGC TGCATGTGGC CTGCCAGAAT 
GGTCACAAGA CGACAGTGCA ATGCTTGCTG GACAGTGGTG CTGATATTAA CAGACCAAAT 
GTATCAGGAG CCACTCCACT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAG 
ATTCTTCTAT TACGAGGTGC CAAGTATCTG TCAGATAAAA ACGGAGTAAC CCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACC TGTGAAGTAT TAATTCAGTA TCACCCTAGG 
CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ATTTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCCACAAC AAATGGTCAT AAATTGCTTA GCATATCTAG TAATTACGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT 
CCATCCTCTC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGGAGC 
CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA 
GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTGCTGAAA 
CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAGGG GAATCTAAAC CAGATGTGCT TGGAGGGAAA 
CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACTTCGCCC 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAACAGGAAT 
CCCAAGATTA TATTTGACCA CTTTCACTTC CTTCTCGAAT GTCCTGAATT GATGTCAAGA 
TTCATGCATA TCATAAAGGC ACAGCCATTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGACATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ATAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTCC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAACCCA ATAGCAACTC CTCTGTAAAT 
CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TCTGAACCAT 
AGGCAACTGG TGAACATTTA CTTCACGAGA TCATTCTATA AGCACATTCT TGGTATTCCT 
GTGAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAACTT GCAATGGATT 
TTAGATAATG ATATTAGTGA TTTGGGTCTA GAATTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA CCTGGGGGTG GAAGTATTCT TGTAACACAA 
AATAACAAAG CGGAGTACGT CCAGCTTGTC ACTGAACTGC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCATC CCTCATACAA 
CTTTTTGATG AATACGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTGGA AGATATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT 
GTTACTGGCA GCCGTGAAGA GATACCTCAC GTCCAAGTTC CAGGGTCCCA CATGGTGGAT 
TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTCAC AATTGCTGCT GTGCCATATA 
CTCCAAATCT TCTACCTACT TCAAGCACAT GCATCAACAT GCTCAAGTTA CCTGAATACC 
CAAGCAAAGA AATACTCAAG GACAGGCTTC TTGTGGCACT ACATTGTGGC AGCTATGGTT 
ACACAATGGC ATAATGAAGT CTGGAAAATT CTTCTGACTG CTGATGAGCA ATTCAGAGTG 
GCAGAAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852437.1
CDS	499..3213
Translation	MRNPSAPVYRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSREEIPHVQVPGSHMVDLLISWVEVDCKTSQLLLCHILQIFYLLQAHASTCSSYLNTQAKKYSRTGFLWHYIVAAMVTQWHNEVWKILLTADEQFRVAEVI

Target ORF information:

RefSeq Version	XM_024996669.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996669.1

ORF Insert Sequence:

ATGAGGAACC CTTCAGCACC AGTTTATAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCAG TGGAATGCTT GGTTCTGCTT TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCGG GCTGTACACC TCTTCATTTG GCAGCTAGAA ATGGGCAAAA GAAATGCATG 
AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG TCTTACTGCA 
ATACACTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT ACAACACGTC 
AGTGATGTCG ATGTTGAGGA TGCGATGGGG CAGACAGCGC TGCATGTGGC CTGCCAGAAT 
GGTCACAAGA CGACAGTGCA ATGCTTGCTG GACAGTGGTG CTGATATTAA CAGACCAAAT 
GTATCAGGAG CCACTCCACT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAG 
ATTCTTCTAT TACGAGGTGC CAAGTATCTG TCAGATAAAA ACGGAGTAAC CCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACC TGTGAAGTAT TAATTCAGTA TCACCCTAGG 
CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ATTTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCCACAAC AAATGGTCAT AAATTGCTTA GCATATCTAG TAATTACGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT 
CCATCCTCTC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGGAGC 
CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA 
GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTGCTGAAA 
CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAGGG GAATCTAAAC CAGATGTGCT TGGAGGGAAA 
CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACTTCGCCC 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAACAGGAAT 
CCCAAGATTA TATTTGACCA CTTTCACTTC CTTCTCGAAT GTCCTGAATT GATGTCAAGA 
TTCATGCATA TCATAAAGGC ACAGCCATTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGACATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ATAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTCC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAACCCA ATAGCAACTC CTCTGTAAAT 
CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TCTGAACCAT 
AGGCAACTGG TGAACATTTA CTTCACGAGA TCATTCTATA AGCACATTCT TGGTATTCCT 
GTGAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAACTT GCAATGGATT 
TTAGATAATG ATATTAGTGA TTTGGGTCTA GAATTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA CCTGGGGGTG GAAGTATTCT TGTAACACAA 
AATAACAAAG CGGAGTACGT CCAGCTTGTC ACTGAACTGC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCATC CCTCATACAA 
CTTTTTGATG AATACGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTGGA AGATATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT 
GTTACTGGCA GCCGTGAAGA GATACCTCAC GTCCAAGTTC CAGGGTCCCA CATGGTGGAT 
TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTCAC AATTGCTGCT GTGCCATATA 
CTCCAAATCT TCTACCTACT TCAAGCACAT GCATCAACAT GCTCAAGTTA CCTGAATACC 
CAAGCAAAGA AATACTCAAG GACAGGCTTC TTGTGGCACT ACATTGTGGC AGCTATGGTT 
ACACAATGGC ATAATGAAGT CTGGAAAATT CTTCTGACTG CTGATGAGCA ATTCAGAGTG 
GCAGAAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_024996670.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852438.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa237786
Clone ID Related Accession (Same CDS sequence)	XM_024996670.1
Accession Version	XM_024996670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG 
GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG 
TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC 
TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG 
AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT 
GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT 
AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG 
AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG 
GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG 
CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC 
TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG 
GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC 
CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT 
AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG 
TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT 
TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT 
GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA 
TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA 
GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA 
CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC 
ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT 
CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GCCGTGAAGA GATACCTCAC 
GTCCAAGTTC CAGGGTCCCA CATGGTGGAT TTGCTAATAT CATGGGTGGA AGTGGATTGC 
AAAACTTCAC AATTGCTGCT GTGCCATATA CTCCAAATCT TCTACCTACT TCAAGCACAT 
GCATCAACAT GCTCAAGTTA CCTGAATACC CAAGCAAAGA AATACTCAAG GACAGGCTTC 
TTGTGGCACT ACATTGTGGC AGCTATGGTT ACACAATGGC ATAATGAAGT CTGGAAAATT 
CTTCTGACTG CTGATGAGCA ATTCAGAGTG GCAGAAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852438.1
CDS	239..2923
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTATVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSREEIPHVQVPGSHMVDLLISWVEVDCKTSQLLLCHILQIFYLLQAHASTCSSYLNTQAKKYSRTGFLWHYIVAAMVTQWHNEVWKILLTADEQFRVAEVI

Target ORF information:

RefSeq Version	XM_024996670.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996670.1

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA 
CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG 
GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG 
TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC 
TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG 
AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT 
GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT 
AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG 
AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG 
GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG 
CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC 
TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG 
GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC 
CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT 
AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG 
TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT 
TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT 
GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA 
TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA 
GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA 
CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC 
ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT 
CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GCCGTGAAGA GATACCTCAC 
GTCCAAGTTC CAGGGTCCCA CATGGTGGAT TTGCTAATAT CATGGGTGGA AGTGGATTGC 
AAAACTTCAC AATTGCTGCT GTGCCATATA CTCCAAATCT TCTACCTACT TCAAGCACAT 
GCATCAACAT GCTCAAGTTA CCTGAATACC CAAGCAAAGA AATACTCAAG GACAGGCTTC 
TTGTGGCACT ACATTGTGGC AGCTATGGTT ACACAATGGC ATAATGAAGT CTGGAAAATT 
CTTCTGACTG CTGATGAGCA ATTCAGAGTG GCAGAAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_024996671.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852439.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa237787
Clone ID Related Accession (Same CDS sequence)	XM_024996671.1
Accession Version	XM_024996671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAC AGATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT 
GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG 
GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT 
AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA 
GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA 
ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG 
TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA 
AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG 
ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT 
AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC 
TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA 
ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG 
CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT 
ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT 
CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG 
CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC 
CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA 
ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT 
GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA 
TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG 
TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT 
GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG 
TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA 
GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT 
CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC 
TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA 
GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT 
CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC 
TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT 
GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT 
CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA 
AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA 
TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA 
ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT 
CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT 
CTCTTGCAGT TTGTTACTGG CAGCCGTGAA GAGATACCTC ACGTCCAAGT TCCAGGGTCC 
CACATGGTGG ATTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG 
CTGTGCCATA TACTCCAAAT CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT 
TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG 
GCAGCTATGG TTACACAATG GCATAATGAA GTCTGGAAAA TTCTTCTGAC TGCTGATGAG 
CAATTCAGAG TGGCAGAAGT AATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852439.1
CDS	85..2511
Translation	MAVQIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSREEIPHVQVPGSHMVDLLISWVEVDCKTSQLLLCHILQIFYLLQAHASTCSSYLNTQAKKYSRTGFLWHYIVAAMVTQWHNEVWKILLTADEQFRVAEVI

Target ORF information:

RefSeq Version	XM_024996671.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996671.1

ORF Insert Sequence:

ATGGCTGTAC AGATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT 
GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG 
GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT 
AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA 
GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA 
ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG 
TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA 
AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG 
ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT 
AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC 
TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA 
ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG 
CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT 
ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT 
CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG 
CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC 
CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA 
ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT 
GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA 
TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG 
TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT 
GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG 
TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA 
GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT 
CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC 
TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA 
GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT 
CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC 
TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT 
GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT 
CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA 
AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA 
TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA 
ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT 
CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT 
CTCTTGCAGT TTGTTACTGG CAGCCGTGAA GAGATACCTC ACGTCCAAGT TCCAGGGTCC 
CACATGGTGG ATTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG 
CTGTGCCATA TACTCCAAAT CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT 
TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG 
GCAGCTATGG TTACACAATG GCATAATGAA GTCTGGAAAA TTCTTCTGAC TGCTGATGAG 
CAATTCAGAG TGGCAGAAGT AATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_024996672.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852440.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa237788
Clone ID Related Accession (Same CDS sequence)	XM_024996672.1
Accession Version	XM_024996672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGAC AGAACTACTC CATGACCTTG TACAACACGT CAGTGATGTC GATGTTGAGG 
ATGCGATGGG GCAGACAGCG CTGCATGTGG CCTGCCAGAA TGGTCACAAG ACGTCATGGT 
CAGAGAGACA CAGCACAGAT TCTTCTATTA CGAGGTGCCA AGTATCTGTC AGATAAAAAC 
GGAGTAACCC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACCTG TGAAGTATTA 
ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAT 
TTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGTCATAA ATTGCTTAGC 
ATATCTAGTA ATTACGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG GATATTTTGT 
CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGGAGCCA GGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT 
GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC 
ACTTCTATTT TGCTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAGGGGA ATCTAAACCA 
GATGTGCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCAGGAATGA CTTCGCCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ACAGGAATCC CAAGATTATA TTTGACCACT TTCACTTCCT TCTCGAATGT 
CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAGGCAC AGCCATTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGACATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAT AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTCCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAACCCAAT 
AGCAACTCCT CTGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG 
GGATTAGCTC TGAACCATAG GCAACTGGTG AACATTTACT TCACGAGATC ATTCTATAAG 
CACATTCTTG GTATTCCTGT GAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC 
AAAAACTTGC AATGGATTTT AGATAATGAT ATTAGTGATT TGGGTCTAGA ATTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CCTTGAAACC TGGGGGTGGA 
AGTATTCTTG TAACACAAAA TAACAAAGCG GAGTACGTCC AGCTTGTCAC TGAACTGCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCATCCC TCATACAACT TTTTGATGAA TACGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ATATTACTCC AGAGGAGAGA 
GTTCTTCTCT TGCAGTTTGT TACTGGCAGC CGTGAAGAGA TACCTCACGT CCAAGTTCCA 
GGGTCCCACA TGGTGGATTT GCTAATATCA TGGGTGGAAG TGGATTGCAA AACTTCACAA 
TTGCTGCTGT GCCATATACT CCAAATCTTC TACCTACTTC AAGCACATGC ATCAACATGC 
TCAAGTTACC TGAATACCCA AGCAAAGAAA TACTCAAGGA CAGGCTTCTT GTGGCACTAC 
ATTGTGGCAG CTATGGTTAC ACAATGGCAT AATGAAGTCT GGAAAATTCT TCTGACTGCT 
GATGAGCAAT TCAGAGTGGC AGAAGTAATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852440.1
CDS	671..2983
Translation	MGGQNYSMTLYNTSVMSMLRMRWGRQRCMWPARMVTRRHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSREEIPHVQVPGSHMVDLLISWVEVDCKTSQLLLCHILQIFYLLQAHASTCSSYLNTQAKKYSRTGFLWHYIVAAMVTQWHNEVWKILLTADEQFRVAEVI

Target ORF information:

RefSeq Version	XM_024996672.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996672.1

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TACAACACGT CAGTGATGTC GATGTTGAGG 
ATGCGATGGG GCAGACAGCG CTGCATGTGG CCTGCCAGAA TGGTCACAAG ACGTCATGGT 
CAGAGAGACA CAGCACAGAT TCTTCTATTA CGAGGTGCCA AGTATCTGTC AGATAAAAAC 
GGAGTAACCC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACCTG TGAAGTATTA 
ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAT 
TTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGTCATAA ATTGCTTAGC 
ATATCTAGTA ATTACGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG GATATTTTGT 
CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGGAGCCA GGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT 
GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC 
ACTTCTATTT TGCTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAGGGGA ATCTAAACCA 
GATGTGCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCAGGAATGA CTTCGCCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ACAGGAATCC CAAGATTATA TTTGACCACT TTCACTTCCT TCTCGAATGT 
CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAGGCAC AGCCATTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGACATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAT AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTCCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAACCCAAT 
AGCAACTCCT CTGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG 
GGATTAGCTC TGAACCATAG GCAACTGGTG AACATTTACT TCACGAGATC ATTCTATAAG 
CACATTCTTG GTATTCCTGT GAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC 
AAAAACTTGC AATGGATTTT AGATAATGAT ATTAGTGATT TGGGTCTAGA ATTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CCTTGAAACC TGGGGGTGGA 
AGTATTCTTG TAACACAAAA TAACAAAGCG GAGTACGTCC AGCTTGTCAC TGAACTGCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCATCCC TCATACAACT TTTTGATGAA TACGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ATATTACTCC AGAGGAGAGA 
GTTCTTCTCT TGCAGTTTGT TACTGGCAGC CGTGAAGAGA TACCTCACGT CCAAGTTCCA 
GGGTCCCACA TGGTGGATTT GCTAATATCA TGGGTGGAAG TGGATTGCAA AACTTCACAA 
TTGCTGCTGT GCCATATACT CCAAATCTTC TACCTACTTC AAGCACATGC ATCAACATGC 
TCAAGTTACC TGAATACCCA AGCAAAGAAA TACTCAAGGA CAGGCTTCTT GTGGCACTAC 
ATTGTGGCAG CTATGGTTAC ACAATGGCAT AATGAAGTCT GGAAAATTCT TCTGACTGCT 
GATGAGCAAT TCAGAGTGGC AGAAGTAATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_024996673.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852441.1 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa237789
Clone ID Related Accession (Same CDS sequence)	XM_024996673.1
Accession Version	XM_024996673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HACE1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGAC AGAACTACTC CATGACCTTG TACAACACGT CAGTGATGTC GATGTTGAGG 
ATGCGATGGG GCAGACAGCG CTGCATGTGG CCTGCCAGAA TGGTCACAAG ACGTCATGGT 
CAGAGAGACA CAGCACAGAT TCTTCTATTA CGAGGTGCCA AGTATCTGTC AGATAAAAAC 
GGAGTAACCC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACCTG TGAAGTATTA 
ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAT 
TTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGTCATAA ATTGCTTAGC 
ATATCTAGTA ATTACGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG GATATTTTGT 
CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGGAGCCA GGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT 
GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC 
ACTTCTATTT TGCTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAGGGGA ATCTAAACCA 
GATGTGCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCAGGAATGA CTTCGCCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ACAGGAATCC CAAGATTATA TTTGACCACT TTCACTTCCT TCTCGAATGT 
CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAGGCAC AGCCATTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGACATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAT AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTCCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAACCCAAT 
AGCAACTCCT CTGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG 
GGATTAGCTC TGAACCATAG GCAACTGGTG AACATTTACT TCACGAGATC ATTCTATAAG 
CACATTCTTG GTATTCCTGT GAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC 
AAAAACTTGC AATGGATTTT AGATAATGAT ATTAGTGATT TGGGTCTAGA ATTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CCTTGAAACC TGGGGGTGGA 
AGTATTCTTG TAACACAAAA TAACAAAGCG GAGTACGTCC AGCTTGTCAC TGAACTGCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCATCCC TCATACAACT TTTTGATGAA TACGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ATATTACTCC AGAGGAGAGA 
GTTCTTCTCT TGCAGTTTGT TACTGGCAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG CTGTGCCATA TACTCCAAAT 
CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGCAAA 
GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852441.1
CDS	671..2896
Translation	MGGQNYSMTLYNTSVMSMLRMRWGRQRCMWPARMVTRRHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSTGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_024996673.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996673.1

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TACAACACGT CAGTGATGTC GATGTTGAGG 
ATGCGATGGG GCAGACAGCG CTGCATGTGG CCTGCCAGAA TGGTCACAAG ACGTCATGGT 
CAGAGAGACA CAGCACAGAT TCTTCTATTA CGAGGTGCCA AGTATCTGTC AGATAAAAAC 
GGAGTAACCC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACCTG TGAAGTATTA 
ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAT 
TTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGTCATAA ATTGCTTAGC 
ATATCTAGTA ATTACGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG GATATTTTGT 
CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGGAGCCA GGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT 
GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC 
ACTTCTATTT TGCTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAGGGGA ATCTAAACCA 
GATGTGCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCAGGAATGA CTTCGCCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ACAGGAATCC CAAGATTATA TTTGACCACT TTCACTTCCT TCTCGAATGT 
CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAGGCAC AGCCATTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGACATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAT AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTCCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAACCCAAT 
AGCAACTCCT CTGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG 
GGATTAGCTC TGAACCATAG GCAACTGGTG AACATTTACT TCACGAGATC ATTCTATAAG 
CACATTCTTG GTATTCCTGT GAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC 
AAAAACTTGC AATGGATTTT AGATAATGAT ATTAGTGATT TGGGTCTAGA ATTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CCTTGAAACC TGGGGGTGGA 
AGTATTCTTG TAACACAAAA TAACAAAGCG GAGTACGTCC AGCTTGTCAC TGAACTGCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCATCCC TCATACAACT TTTTGATGAA TACGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ATATTACTCC AGAGGAGAGA 
GTTCTTCTCT TGCAGTTTGT TACTGGCAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG CTGTGCCATA TACTCCAAAT 
CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGCAAA 
GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HACE1
Entrez Gene ID	527565
Full Name	HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1
General protein information	Preferred Names HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase HACE1 HECT-type E3 ubiquitin transferase HACE1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	HACE1	NP_065822.2
Canis lupus familiaris (dog)	HACE1	XP_854232.1
Pan troglodytes (chimpanzee)	HACE1	XP_518654.2
Rattus norvegicus (Norway rat)	Hace1	NP_001102009.2
Gallus gallus (chicken)	HACE1	NP_001265084.1
Xenopus tropicalis (tropical clawed frog)	hace1	NP_001039269.1
Bos taurus (cattle)	HACE1	NP_001179574.2
Mus musculus (house mouse)	Hace1	NP_766061.2
Danio rerio (zebrafish)	hace1	XP_701235.5

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HACE1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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