Col6a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col6a2
Entrez Gene ID	361821
Full Name	collagen type VI alpha 2 chain
General protein information	Preferred Names collagen type VI alpha 2 chain Names collagen alpha-2(VI) chain collagen, type VI, alpha 2 procollagen, type VI, alpha 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100741.1	NP_001094211.1	collagen alpha-2(VI) chain precursor
XM_006256300.3	XP_006256362.1	collagen alpha-2(VI) chain isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL6A2	XP_531504.3
Gallus gallus (chicken)	COL6A2	NP_990679.1
Homo sapiens (human)	COL6A2	NP_001840.3
Mus musculus (house mouse)	Col6a2	NP_666119.1
Xenopus tropicalis (tropical clawed frog)	LOC100145523	NP_001120436.1
Danio rerio (zebrafish)	col6a2	XP_696164.2
	COL6A2	XP_001099130.2
Rattus norvegicus (Norway rat)	Col6a2	NP_001094211.1

Gene Ontology
Bibliography

Related articles in PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Chiasserini D, van Weering JR, Piersma SR, Pham TV, Malekzadeh A, Teunissen CE, de Wit H, Jim?nez CR
Journal of proteomics106191-204(2014 Jun)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
Soteriou D, Iskender B, Byron A, Humphries JD, Borg-Bartolo S, Haddock MC, Baxter MA, Knight D, Humphries MJ, Kimber SJ
The Journal of biological chemistry288(26)18716-31(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Col6a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col6a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa39365	XM_006256300.3 Latest version!	Rattus norvegicus collagen, type VI, alpha 2 (Col6a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa07712	NM_001100741.1 Latest version!	Rattus norvegicus collagen type VI alpha 2 chain (Col6a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col6a2 ( XM_006256300.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256362.1 Rattus norvegicus collagen, type VI, alpha 2 (Col6a2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39365
Clone ID Related Accession (Same CDS sequence)	XM_006256300.3
Accession Version	XM_006256300.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-2(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256300.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACTA CTACCAAGAT GCTCCAGGGT TCTTTTTCTG TGCTCCTGCT TGGGGGACTC 
TTGGGGGTCC TCCATGCCCA GCAGCAGGAA GCCATCTCAC CTGATATCTC TACTACTGAC 
AGGAACAACA ACTGTCCAGA GAAGGCTGAC TGCCCAGTCA ACGTGTACTT CGTGTTGGAC 
ACCTCGGAGA GCGTGGCCAT GCAGTCCCCC ACAGACAGCC TGCTTTATCA CATGCAGCAG 
TTCGTACCAC AATTTATCAG CCAGCTGCAG AACGAGTTCT ACCTGGAGCA GGTGGCCCTG 
AGCTGGCGCT ATGGTGGCCT ACACTTCTCG GACCAGGTGG AGGTGTTCAG CCCCCCAGGC 
AGCGACCGGG CCTCCTTCAC TAAGAGCCTA CAAGGCATCC GCTCCTTCCG CAGGGGCACC 
TTCACTGACT GTGCCTTGGC CAACATGACC CAGCAGATCC GGCAGCACGT AGGCCGGGGT 
GTAGTCAACT TTGCCGTGGT CATCACCGAT GGCCACGTCA CAGGCAATCC CTGTGGGGGC 
ATCAAAATGC AGGCTGAGCG TGCCCGCGAG GAGGGCATCC GGCTCTTTGC CGTGGCCCCC 
AATAGGAACC TAAATGAACA AGGCCTGCGG GATATCGCCA ACACCCCACA TGAGCTATAC 
CGAAACAACT ATGCCACCAT GCGACCTGAC TCTACTGAGA TTGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TACGGAGAGT GCTACAAGGT GAGCTGCCTG 
GAGATTCCGG GACCCCACGG ACCCAAGGGT TACCGAGGAC AGAAGGGCGC CAAGGGCAAC 
ATGGGCGAGC CAGGAGAACC TGGACAGAAA GGACGACAGG GAGACCCTGG CATTGAAGGC 
CCCATTGGAT TCCCAGGACC CAAGGGTGTG CCTGGCTTCA AGGGAGAGAA GGGTGAATTC 
GGATCAGATG GTCGGAAGGG AGCTCCGGGT CTAGCAGGCA AGAACGGAAC AGATGGACAG 
AAGGGCAAAC TGGGCCGTAT TGGGCCTCCT GGTTGCAAGG GAGACCCGGG AAGTCGGGGC 
CCCGATGGAT ACCCCGGAGA AGCTGGAAGC CCGGGAGAGC AAGGAGACCA AGGTGCCAAG 
GGGGACTCTG GCCGCCCAGG ACGCAGGGGA CCACCAGGAA ATCCTGGAGA CAAAGGAAGC 
AAGGGATATC GAGGCAACAG TGGAGCTCCC GGAAGTCCAG GAGTGAAAGG AGGCAAGGGA 
GGGCCTGGCC CCCGTGGACC AAAAGGAGAG CCTGGACGCA GGGGAGATCC TGGGACCAAG 
GGCGGCCCGG GCAGTGATGG TCCAAAGGGG GAGAAGGGAG ACCCTGGTCC TGAGGGGCCA 
CGAGGTCTGG CTGGAGAAAT CGGCAGTAAA GGAGCCAAGG GAGACAGAGG TTTGCCTGGA 
CCCAGAGGCC CCCAGGGAGC TCTTGGGGAG CCAGGGAAGC AGGGATCTCG GGGAGACCCT 
GGGGATGCTG GGCCTCGAGG AGATTCAGGA CAGCCAGGAC CCAAGGGAGA TCCTGGAAGG 
CCTGGATTCA GCTACCCAGG ACCCCGAGGG ACACCCGGTG AAAAAGGCGA GCCCGGCCCA 
CCAGGCCCTG AGGGAGGCCG AGGAGACTTT GGTCTGAAAG GAGCACCCGG ACGGAAGGGA 
GAAAAAGGAG AGCCAGCTGA TCCTGGTCCC CCTGGTGAAC CTGGCCCTCG GGGGCCAAGA 
GGAATCCCAG GACCTGAGGG AGAACCCGGC CCTCCAGGAG ATCCTGGTCT CACGGAATGT 
GACGTCATGA CCTATGTGAG GGAGACCTGT GGGTGCTGTG ACTGTGAGAA GCGCTGCGGT 
GCCCTGGATG TGGTCTTCGT CATCGACAGC TCTGAGAGTA TTGGCTACAC CAACTTCACC 
TTGGAGAAGA ACTTTGTCAT CAATGTGGTC AACAGGCTGG GCGCCATTGC CAAGGACCCC 
AAGTCTGAAA CGGGTACACG TGTGGGTGTG GTACAGTACA GCCATGAGGG CACCTTTGAG 
GCCATCCGGC TGGACGACGA GCGGGTCAAC TCCCTGTCCA GTTTCAAGGA GGCCGTCAAA 
AACCTTGAGT GGATTGCCGG CGGCACTTGG ACGCCCTCTG CCCTCAAGTT CGCCTATAAT 
CAGCTCATCA AAGAGAGCCG GCGCCAGAAG ACCCGGGTGT TCGCAGTGGT CATCACAGAT 
GGGCGCCATG ACCCTCGAGA TGATGACCTC AATCTTCGGG CACTGTGTGA CCGAGACGTC 
ACCGTGACAG CCATCGGCAT CGGTGACATG TTCCACGAGA CCCACGAGAG CGAGAACCTC 
TACTCCATCG CCTGTGACAA GCCCCAGCAG GTGCGCAACA TGACGCTGTT CTCTGACCTG 
GTGGCCGAGA AATTCATCGA TGACATGGAA GACGTCCTTT GTCCGGACCC CCAGATCGTG 
TGTCCAGAAC TTCCCTGCCA AACAGATGAA CCATGGCCCG GGAACAAGCC CCCGGTCACC 
TTCCTCCGCA CGGAAGAGGG TCCAGACCCC ACCTTCCCCA AGACCATCCC CCTGATCCAG 
CAGTTGCTAA ACGCCACGGA GTTCACACAG AACCCAGCTG CCTACTCCCA ACTGGTGGCT 
GTGATGGTCT ACACCGCCGA GAGGGCCAAG TTCTCCACAG GGGTCGAGCG GCAGGACTGG 
ATGCAGCTGT TCATTGACAC CTTTAAACTG GTGCACAGGG ATATCACAGG GGACCCAGAG 
AGCGTGCTGG CACTCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256362.1
CDS	128..2908
Translation	MTTTTKMLQGSFSVLLLGGLLGVLHAQQQEAISPDISTTDRNNNCPEKADCPVNVYFVLDTSESVAMQSPTDSLLYHMQQFVPQFISQLQNEFYLEQVALSWRYGGLHFSDQVEVFSPPGSDRASFTKSLQGIRSFRRGTFTDCALANMTQQIRQHVGRGVVNFAVVITDGHVTGNPCGGIKMQAERAREEGIRLFAVAPNRNLNEQGLRDIANTPHELYRNNYATMRPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPHGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGSDGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDPGSRGPDGYPGEAGSPGEQGDQGAKGDSGRPGRRGPPGNPGDKGSKGYRGNSGAPGSPGVKGGKGGPGPRGPKGEPGRRGDPGTKGGPGSDGPKGEKGDPGPEGPRGLAGEIGSKGAKGDRGLPGPRGPQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGTPGEKGEPGPPGPEGGRGDFGLKGAPGRKGEKGEPADPGPPGEPGPRGPRGIPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIRLDDERVNSLSSFKEAVKNLEWIAGGTWTPSALKFAYNQLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHETHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPELPCQTDEPWPGNKPPVTFLRTEEGPDPTFPKTIPLIQQLLNATEFTQNPAAYSQLVAVMVYTAERAKFSTGVERQDWMQLFIDTFKLVHRDITGDPESVLALC

Target ORF information:

RefSeq Version	XM_006256300.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type VI, alpha 2 (Col6a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256300.3

ORF Insert Sequence:

ATGACGACTA CTACCAAGAT GCTCCAGGGT TCTTTTTCTG TGCTCCTGCT TGGGGGACTC 
TTGGGGGTCC TCCATGCCCA GCAGCAGGAA GCCATCTCAC CTGATATCTC TACTACTGAC 
AGGAACAACA ACTGTCCAGA GAAGGCTGAC TGCCCAGTCA ACGTGTACTT CGTGTTGGAC 
ACCTCGGAGA GCGTGGCCAT GCAGTCCCCC ACAGACAGCC TGCTTTATCA CATGCAGCAG 
TTCGTACCAC AATTTATCAG CCAGCTGCAG AACGAGTTCT ACCTGGAGCA GGTGGCCCTG 
AGCTGGCGCT ATGGTGGCCT ACACTTCTCG GACCAGGTGG AGGTGTTCAG CCCCCCAGGC 
AGCGACCGGG CCTCCTTCAC TAAGAGCCTA CAAGGCATCC GCTCCTTCCG CAGGGGCACC 
TTCACTGACT GTGCCTTGGC CAACATGACC CAGCAGATCC GGCAGCACGT AGGCCGGGGT 
GTAGTCAACT TTGCCGTGGT CATCACCGAT GGCCACGTCA CAGGCAATCC CTGTGGGGGC 
ATCAAAATGC AGGCTGAGCG TGCCCGCGAG GAGGGCATCC GGCTCTTTGC CGTGGCCCCC 
AATAGGAACC TAAATGAACA AGGCCTGCGG GATATCGCCA ACACCCCACA TGAGCTATAC 
CGAAACAACT ATGCCACCAT GCGACCTGAC TCTACTGAGA TTGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TACGGAGAGT GCTACAAGGT GAGCTGCCTG 
GAGATTCCGG GACCCCACGG ACCCAAGGGT TACCGAGGAC AGAAGGGCGC CAAGGGCAAC 
ATGGGCGAGC CAGGAGAACC TGGACAGAAA GGACGACAGG GAGACCCTGG CATTGAAGGC 
CCCATTGGAT TCCCAGGACC CAAGGGTGTG CCTGGCTTCA AGGGAGAGAA GGGTGAATTC 
GGATCAGATG GTCGGAAGGG AGCTCCGGGT CTAGCAGGCA AGAACGGAAC AGATGGACAG 
AAGGGCAAAC TGGGCCGTAT TGGGCCTCCT GGTTGCAAGG GAGACCCGGG AAGTCGGGGC 
CCCGATGGAT ACCCCGGAGA AGCTGGAAGC CCGGGAGAGC AAGGAGACCA AGGTGCCAAG 
GGGGACTCTG GCCGCCCAGG ACGCAGGGGA CCACCAGGAA ATCCTGGAGA CAAAGGAAGC 
AAGGGATATC GAGGCAACAG TGGAGCTCCC GGAAGTCCAG GAGTGAAAGG AGGCAAGGGA 
GGGCCTGGCC CCCGTGGACC AAAAGGAGAG CCTGGACGCA GGGGAGATCC TGGGACCAAG 
GGCGGCCCGG GCAGTGATGG TCCAAAGGGG GAGAAGGGAG ACCCTGGTCC TGAGGGGCCA 
CGAGGTCTGG CTGGAGAAAT CGGCAGTAAA GGAGCCAAGG GAGACAGAGG TTTGCCTGGA 
CCCAGAGGCC CCCAGGGAGC TCTTGGGGAG CCAGGGAAGC AGGGATCTCG GGGAGACCCT 
GGGGATGCTG GGCCTCGAGG AGATTCAGGA CAGCCAGGAC CCAAGGGAGA TCCTGGAAGG 
CCTGGATTCA GCTACCCAGG ACCCCGAGGG ACACCCGGTG AAAAAGGCGA GCCCGGCCCA 
CCAGGCCCTG AGGGAGGCCG AGGAGACTTT GGTCTGAAAG GAGCACCCGG ACGGAAGGGA 
GAAAAAGGAG AGCCAGCTGA TCCTGGTCCC CCTGGTGAAC CTGGCCCTCG GGGGCCAAGA 
GGAATCCCAG GACCTGAGGG AGAACCCGGC CCTCCAGGAG ATCCTGGTCT CACGGAATGT 
GACGTCATGA CCTATGTGAG GGAGACCTGT GGGTGCTGTG ACTGTGAGAA GCGCTGCGGT 
GCCCTGGATG TGGTCTTCGT CATCGACAGC TCTGAGAGTA TTGGCTACAC CAACTTCACC 
TTGGAGAAGA ACTTTGTCAT CAATGTGGTC AACAGGCTGG GCGCCATTGC CAAGGACCCC 
AAGTCTGAAA CGGGTACACG TGTGGGTGTG GTACAGTACA GCCATGAGGG CACCTTTGAG 
GCCATCCGGC TGGACGACGA GCGGGTCAAC TCCCTGTCCA GTTTCAAGGA GGCCGTCAAA 
AACCTTGAGT GGATTGCCGG CGGCACTTGG ACGCCCTCTG CCCTCAAGTT CGCCTATAAT 
CAGCTCATCA AAGAGAGCCG GCGCCAGAAG ACCCGGGTGT TCGCAGTGGT CATCACAGAT 
GGGCGCCATG ACCCTCGAGA TGATGACCTC AATCTTCGGG CACTGTGTGA CCGAGACGTC 
ACCGTGACAG CCATCGGCAT CGGTGACATG TTCCACGAGA CCCACGAGAG CGAGAACCTC 
TACTCCATCG CCTGTGACAA GCCCCAGCAG GTGCGCAACA TGACGCTGTT CTCTGACCTG 
GTGGCCGAGA AATTCATCGA TGACATGGAA GACGTCCTTT GTCCGGACCC CCAGATCGTG 
TGTCCAGAAC TTCCCTGCCA AACAGATGAA CCATGGCCCG GGAACAAGCC CCCGGTCACC 
TTCCTCCGCA CGGAAGAGGG TCCAGACCCC ACCTTCCCCA AGACCATCCC CCTGATCCAG 
CAGTTGCTAA ACGCCACGGA GTTCACACAG AACCCAGCTG CCTACTCCCA ACTGGTGGCT 
GTGATGGTCT ACACCGCCGA GAGGGCCAAG TTCTCCACAG GGGTCGAGCG GCAGGACTGG 
ATGCAGCTGT TCATTGACAC CTTTAAACTG GTGCACAGGG ATATCACAGG GGACCCAGAG 
AGCGTGCTGG CACTCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a2 ( NM_001100741.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094211.1 Rattus norvegicus collagen type VI alpha 2 chain (Col6a2), mRNA.

Price & Availability↑

CloneID	ORa07712
Clone ID Related Accession (Same CDS sequence)	NM_001100741.1
Accession Version	NM_001100741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-2(VI) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM036514.1, FM048712.1, CK365920.1, DN935075.1, CB616438.1, FM093132.1 and BC089923.1. On or before Dec 20, 2009 this sequence version replaced XM_001079642.1, XM_342115.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END##

ATGACGACTA CTACCAAGAT GCTCCAGGGT TCTTTTTCTG TGCTCCTGCT TGGGGGACTC 
TTGGGGGTCC TCCATGCCCA GCAGCAGGAA GCCATCTCAC CTGATATCTC TACTACTGAC 
AGGAACAACA ACTGTCCAGA GAAGGCTGAC TGCCCAGTCA ACGTGTACTT CGTGTTGGAC 
ACCTCGGAGA GCGTGGCCAT GCAGTCCCCC ACAGACAGCC TGCTTTATCA CATGCAGCAG 
TTCGTACCAC AATTTATCAG CCAGCTGCAG AACGAGTTCT ACCTGGAGCA GGTGGCCCTG 
AGCTGGCGCT ATGGTGGCCT ACACTTCTCG GACCAGGTGG AGGTGTTCAG CCCCCCAGGC 
AGCGACCGGG CCTCCTTCAC TAAGAGCCTA CAAGGCATCC GCTCCTTCCG CAGGGGCACC 
TTCACTGACT GTGCCTTGGC CAACATGACC CAGCAGATCC GGCAGCACGT AGGCCGGGGT 
GTAGTCAACT TTGCCGTGGT CATCACCGAT GGCCACGTCA CAGGCAATCC CTGTGGGGGC 
ATCAAAATGC AGGCTGAGCG TGCCCGCGAG GAGGGCATCC GGCTCTTTGC CGTGGCCCCC 
AATAGGAACC TAAATGAACA AGGCCTGCGG GATATCGCCA ACACCCCACA TGAGCTATAC 
CGAAACAACT ATGCCACCAT GCGACCTGAC TCTACTGAGA TTGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TACGGAGAGT GCTACAAGGT GAGCTGCCTG 
GAGATTCCGG GACCCCACGG ACCCAAGGGT TACCGAGGAC AGAAGGGCGC CAAGGGCAAC 
ATGGGCGAGC CAGGAGAACC TGGACAGAAA GGACGACAGG GAGACCCTGG CATTGAAGGC 
CCCATTGGAT TCCCAGGACC CAAGGGTGTG CCTGGCTTCA AGGGAGAGAA GGGTGAATTC 
GGATCAGATG GTCGGAAGGG AGCTCCGGGT CTAGCAGGCA AGAACGGAAC AGATGGACAG 
AAGGGCAAAC TGGGCCGTAT TGGGCCTCCT GGTTGCAAGG GAGACCCGGG AAGTCGGGGC 
CCCGATGGAT ACCCCGGAGA AGCTGGAAGC CCGGGAGAGC AAGGAGACCA AGGTGCCAAG 
GGGGACTCTG GCCGCCCAGG ACGCAGGGGA CCACCAGGAA ATCCTGGAGA CAAAGGAAGC 
AAGGGATATC GAGGCAACAG TGGAGCTCCC GGAAGTCCAG GAGTGAAAGG AGGCAAGGGA 
GGGCCTGGCC CCCGTGGACC AAAAGGAGAG CCTGGACGCA GGGGAGATCC TGGGACCAAG 
GGCGGCCCGG GCAGTGATGG TCCAAAGGGG GAGAAGGGAG ACCCTGGTCC TGAGGGGCCA 
CGAGGTCTGG CTGGAGAAAT CGGCAGTAAA GGAGCCAAGG GAGACAGAGG TTTGCCTGGA 
CCCAGAGGCC CCCAGGGAGC TCTTGGGGAG CCAGGGAAGC AGGGATCTCG GGGAGACCCT 
GGGGATGCTG GGCCTCGAGG AGATTCAGGA CAGCCAGGAC CCAAGGGAGA TCCTGGAAGG 
CCTGGATTCA GCTACCCAGG ACCCCGAGGG ACACCCGGTG AAAAAGGCGA GCCCGGCCCA 
CCAGGCCCTG AGGGAGGCCG AGGAGACTTT GGTCTGAAAG GAGCACCCGG ACGGAAGGGA 
GAAAAAGGAG AGCCAGCTGA TCCTGGTCCC CCTGGTGAAC CTGGCCCTCG GGGGCCAAGA 
GGAATCCCAG GACCTGAGGG AGAACCCGGC CCTCCAGGAG ATCCTGGTCT CACGGAATGT 
GACGTCATGA CCTATGTGAG GGAGACCTGT GGGTGCTGTG ACTGTGAGAA GCGCTGCGGT 
GCCCTGGATG TGGTCTTCGT CATCGACAGC TCTGAGAGTA TTGGCTACAC CAACTTCACC 
TTGGAGAAGA ACTTTGTCAT CAATGTGGTC AACAGGCTGG GCGCCATTGC CAAGGACCCC 
AAGTCTGAAA CGGGTACACG TGTGGGTGTG GTACAGTACA GCCATGAGGG CACCTTTGAG 
GCCATCCGGC TGGACGACGA GCGGGTCAAC TCCCTGTCCA GTTTCAAGGA GGCCGTCAAA 
AACCTTGAGT GGATTGCCGG CGGCACTTGG ACGCCCTCTG CCCTCAAGTT CGCCTATAAT 
CAGCTCATCA AAGAGAGCCG GCGCCAGAAG ACCCGGGTGT TCGCAGTGGT CATCACAGAT 
GGGCGCCATG ACCCTCGAGA TGATGACCTC AATCTTCGGG CACTGTGTGA CCGAGACGTC 
ACCGTGACAG CCATCGGCAT CGGTGACATG TTCCACGAGA CCCACGAGAG CGAGAACCTC 
TACTCCATCG CCTGTGACAA GCCCCAGCAG GTGCGCAACA TGACGCTGTT CTCTGACCTG 
GTGGCCGAGA AATTCATCGA TGACATGGAA GACGTCCTTT GTCCGGACCC CCAGATCGTG 
TGTCCAGAAC TTCCCTGCCA AACAGAGCTC TATGTGGCCC AGTGCACACA ACGGCCCGTG 
GACATTGTCT TCCTGCTGGA TGGCTCGGAG CGGCTGGGCG AGCAGAACTT CTACAAGGCA 
CGGCGCTTCG TGGAGGAGGT GTCACGGCGC CTGACTCTGG CACGAAGGGA TGACGACCCA 
CTCAATGCCC GCATGGCCCT GTTGCAGTAT GGAAGCCAGA ACCAGCAACA GGTGGCCTTC 
CCGTTGACCT ACAATGTGAC CACCATCCAC GAGGCCCTGG AGAGAACCAC CTACCTCAAT 
TCTTTCTCGC ACGTGGGCAC AGGCATCGTG CACGCCATCA ACAACGTGGT GCGGGGGGCA 
CGGGGTGGGG CCCGGCGTCA CGCGGAGCTG TCCTTCGTCT TCCTCACGGA CGGCGTCACG 
GGCAACGACA GCCTGGAGGA GTCCGTACAC TCCATGCGTA AGCAGAATGT GGTGCCCACC 
GTGGTCGCTG TGGGTGGTGA TGTGGACATG GATGTACTTA CCAAGATCAG CCTGGGTGAC 
AGGGCGGCCA TCTTCCGGGA GAAAGACTTC GACAGTCTAG CCCAGCCCAG CTTCTTCGAC 
AGGTTCATCC GCTGGATCTG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094211.1
CDS	80..3163
Translation	MTTTTKMLQGSFSVLLLGGLLGVLHAQQQEAISPDISTTDRNNNCPEKADCPVNVYFVLDTSESVAMQSPTDSLLYHMQQFVPQFISQLQNEFYLEQVALSWRYGGLHFSDQVEVFSPPGSDRASFTKSLQGIRSFRRGTFTDCALANMTQQIRQHVGRGVVNFAVVITDGHVTGNPCGGIKMQAERAREEGIRLFAVAPNRNLNEQGLRDIANTPHELYRNNYATMRPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPHGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGSDGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDPGSRGPDGYPGEAGSPGEQGDQGAKGDSGRPGRRGPPGNPGDKGSKGYRGNSGAPGSPGVKGGKGGPGPRGPKGEPGRRGDPGTKGGPGSDGPKGEKGDPGPEGPRGLAGEIGSKGAKGDRGLPGPRGPQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGTPGEKGEPGPPGPEGGRGDFGLKGAPGRKGEKGEPADPGPPGEPGPRGPRGIPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIRLDDERVNSLSSFKEAVKNLEWIAGGTWTPSALKFAYNQLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHETHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPELPCQTELYVAQCTQRPVDIVFLLDGSERLGEQNFYKARRFVEEVSRRLTLARRDDDPLNARMALLQYGSQNQQQVAFPLTYNVTTIHEALERTTYLNSFSHVGTGIVHAINNVVRGARGGARRHAELSFVFLTDGVTGNDSLEESVHSMRKQNVVPTVVAVGGDVDMDVLTKISLGDRAAIFREKDFDSLAQPSFFDRFIRWIC

Target ORF information:

RefSeq Version	NM_001100741.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type VI alpha 2 chain (Col6a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100741.1

ORF Insert Sequence:

ATGACGACTA CTACCAAGAT GCTCCAGGGT TCTTTTTCTG TGCTCCTGCT TGGGGGACTC 
TTGGGGGTCC TCCATGCCCA GCAGCAGGAA GCCATCTCAC CTGATATCTC TACTACTGAC 
AGGAACAACA ACTGTCCAGA GAAGGCTGAC TGCCCAGTCA ACGTGTACTT CGTGTTGGAC 
ACCTCGGAGA GCGTGGCCAT GCAGTCCCCC ACAGACAGCC TGCTTTATCA CATGCAGCAG 
TTCGTACCAC AATTTATCAG CCAGCTGCAG AACGAGTTCT ACCTGGAGCA GGTGGCCCTG 
AGCTGGCGCT ATGGTGGCCT ACACTTCTCG GACCAGGTGG AGGTGTTCAG CCCCCCAGGC 
AGCGACCGGG CCTCCTTCAC TAAGAGCCTA CAAGGCATCC GCTCCTTCCG CAGGGGCACC 
TTCACTGACT GTGCCTTGGC CAACATGACC CAGCAGATCC GGCAGCACGT AGGCCGGGGT 
GTAGTCAACT TTGCCGTGGT CATCACCGAT GGCCACGTCA CAGGCAATCC CTGTGGGGGC 
ATCAAAATGC AGGCTGAGCG TGCCCGCGAG GAGGGCATCC GGCTCTTTGC CGTGGCCCCC 
AATAGGAACC TAAATGAACA AGGCCTGCGG GATATCGCCA ACACCCCACA TGAGCTATAC 
CGAAACAACT ATGCCACCAT GCGACCTGAC TCTACTGAGA TTGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TACGGAGAGT GCTACAAGGT GAGCTGCCTG 
GAGATTCCGG GACCCCACGG ACCCAAGGGT TACCGAGGAC AGAAGGGCGC CAAGGGCAAC 
ATGGGCGAGC CAGGAGAACC TGGACAGAAA GGACGACAGG GAGACCCTGG CATTGAAGGC 
CCCATTGGAT TCCCAGGACC CAAGGGTGTG CCTGGCTTCA AGGGAGAGAA GGGTGAATTC 
GGATCAGATG GTCGGAAGGG AGCTCCGGGT CTAGCAGGCA AGAACGGAAC AGATGGACAG 
AAGGGCAAAC TGGGCCGTAT TGGGCCTCCT GGTTGCAAGG GAGACCCGGG AAGTCGGGGC 
CCCGATGGAT ACCCCGGAGA AGCTGGAAGC CCGGGAGAGC AAGGAGACCA AGGTGCCAAG 
GGGGACTCTG GCCGCCCAGG ACGCAGGGGA CCACCAGGAA ATCCTGGAGA CAAAGGAAGC 
AAGGGATATC GAGGCAACAG TGGAGCTCCC GGAAGTCCAG GAGTGAAAGG AGGCAAGGGA 
GGGCCTGGCC CCCGTGGACC AAAAGGAGAG CCTGGACGCA GGGGAGATCC TGGGACCAAG 
GGCGGCCCGG GCAGTGATGG TCCAAAGGGG GAGAAGGGAG ACCCTGGTCC TGAGGGGCCA 
CGAGGTCTGG CTGGAGAAAT CGGCAGTAAA GGAGCCAAGG GAGACAGAGG TTTGCCTGGA 
CCCAGAGGCC CCCAGGGAGC TCTTGGGGAG CCAGGGAAGC AGGGATCTCG GGGAGACCCT 
GGGGATGCTG GGCCTCGAGG AGATTCAGGA CAGCCAGGAC CCAAGGGAGA TCCTGGAAGG 
CCTGGATTCA GCTACCCAGG ACCCCGAGGG ACACCCGGTG AAAAAGGCGA GCCCGGCCCA 
CCAGGCCCTG AGGGAGGCCG AGGAGACTTT GGTCTGAAAG GAGCACCCGG ACGGAAGGGA 
GAAAAAGGAG AGCCAGCTGA TCCTGGTCCC CCTGGTGAAC CTGGCCCTCG GGGGCCAAGA 
GGAATCCCAG GACCTGAGGG AGAACCCGGC CCTCCAGGAG ATCCTGGTCT CACGGAATGT 
GACGTCATGA CCTATGTGAG GGAGACCTGT GGGTGCTGTG ACTGTGAGAA GCGCTGCGGT 
GCCCTGGATG TGGTCTTCGT CATCGACAGC TCTGAGAGTA TTGGCTACAC CAACTTCACC 
TTGGAGAAGA ACTTTGTCAT CAATGTGGTC AACAGGCTGG GCGCCATTGC CAAGGACCCC 
AAGTCTGAAA CGGGTACACG TGTGGGTGTG GTACAGTACA GCCATGAGGG CACCTTTGAG 
GCCATCCGGC TGGACGACGA GCGGGTCAAC TCCCTGTCCA GTTTCAAGGA GGCCGTCAAA 
AACCTTGAGT GGATTGCCGG CGGCACTTGG ACGCCCTCTG CCCTCAAGTT CGCCTATAAT 
CAGCTCATCA AAGAGAGCCG GCGCCAGAAG ACCCGGGTGT TCGCAGTGGT CATCACAGAT 
GGGCGCCATG ACCCTCGAGA TGATGACCTC AATCTTCGGG CACTGTGTGA CCGAGACGTC 
ACCGTGACAG CCATCGGCAT CGGTGACATG TTCCACGAGA CCCACGAGAG CGAGAACCTC 
TACTCCATCG CCTGTGACAA GCCCCAGCAG GTGCGCAACA TGACGCTGTT CTCTGACCTG 
GTGGCCGAGA AATTCATCGA TGACATGGAA GACGTCCTTT GTCCGGACCC CCAGATCGTG 
TGTCCAGAAC TTCCCTGCCA AACAGAGCTC TATGTGGCCC AGTGCACACA ACGGCCCGTG 
GACATTGTCT TCCTGCTGGA TGGCTCGGAG CGGCTGGGCG AGCAGAACTT CTACAAGGCA 
CGGCGCTTCG TGGAGGAGGT GTCACGGCGC CTGACTCTGG CACGAAGGGA TGACGACCCA 
CTCAATGCCC GCATGGCCCT GTTGCAGTAT GGAAGCCAGA ACCAGCAACA GGTGGCCTTC 
CCGTTGACCT ACAATGTGAC CACCATCCAC GAGGCCCTGG AGAGAACCAC CTACCTCAAT 
TCTTTCTCGC ACGTGGGCAC AGGCATCGTG CACGCCATCA ACAACGTGGT GCGGGGGGCA 
CGGGGTGGGG CCCGGCGTCA CGCGGAGCTG TCCTTCGTCT TCCTCACGGA CGGCGTCACG 
GGCAACGACA GCCTGGAGGA GTCCGTACAC TCCATGCGTA AGCAGAATGT GGTGCCCACC 
GTGGTCGCTG TGGGTGGTGA TGTGGACATG GATGTACTTA CCAAGATCAG CCTGGGTGAC 
AGGGCGGCCA TCTTCCGGGA GAAAGACTTC GACAGTCTAG CCCAGCCCAG CTTCTTCGAC 
AGGTTCATCC GCTGGATCTG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Journal of proteomics106191-204(2014 Jun)
Chiasserini D,van Weering JR,Piersma SR,Pham TV,Malekzadeh A,Teunissen CE,de Wit H,Jim?nez CR

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
The Journal of biological chemistry288(26)18716-31(2013 Jun)
Soteriou D,Iskender B,Byron A,Humphries JD,Borg-Bartolo S,Haddock MC,Baxter MA,Knight D,Humphries MJ,Kimber SJ

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Col6a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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