COL6A2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL6A2
Entrez Gene ID	1292
Full Name	collagen type VI alpha 2 chain
Synonyms	BTHLM1,PP3610,UCMD1
General protein information	Preferred Names collagen type VI alpha 2 chain Names collagen alpha-2(VI) chain collagen VI, alpha-2 polypeptide collagen, type VI, alpha 2 human mRNA for collagen VI alpha-2 C-terminal globular domain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.3
Summary	This gene encodes one of the three alpha chains of type VI collagen, a beaded filament collagen found in most connective tissues. The product of this gene contains several domains similar to von Willebrand Factor type A domains. These domains have been shown to bind extracellular matrix proteins, an interaction that explains the importance of this collagen in organizing matrix components. Mutations in this gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	120240
Disorder Html:	Bethlem myopathy 1, 158810 (3); Ullrich congenital muscular dystrophy 1, 254090 (3); ?Myosclerosis, congenital, 255600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011529452.1	XP_011527754.1	collagen alpha-2(VI) chain isoform X1
XM_011529451.1	XP_011527753.1	collagen alpha-2(VI) chain isoform X1
NM_001849.4	NP_001840.3	collagen alpha-2(VI) chain isoform 2C2 precursor
NM_058175.2	NP_478055.2	collagen alpha-2(VI) chain isoform 2C2a' precursor
NM_001849.3	NP_001840.3	collagen alpha-2(VI) chain isoform 2C2 precursor
NM_058174.3	NP_478054.2	collagen alpha-2(VI) chain isoform 2C2a precursor
NM_058174.2	NP_478054.2	collagen alpha-2(VI) chain isoform 2C2a precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL6A2	XP_531504.3
Gallus gallus (chicken)	COL6A2	NP_990679.1
Homo sapiens (human)	COL6A2	NP_001840.3
Mus musculus (house mouse)	Col6a2	NP_666119.1
Xenopus tropicalis (tropical clawed frog)	LOC100145523	NP_001120436.1
Danio rerio (zebrafish)	col6a2	XP_696164.2
	COL6A2	XP_001099130.2
Rattus norvegicus (Norway rat)	Col6a2	NP_001094211.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic characterization of human multiple myeloma bone marrow extracellular matrix.
Glavey SV, Naba A, Manier S, Clauser K, Tahri S, Park J, Reagan MR, Moschetta M, Mishima Y, Gambella M, Rocci A, Sacco A, O'Dwyer ME, Asara JM, Palumbo A, Roccaro AM, Hynes RO, Ghobrial IM
Leukemia31(11)2426-2434(2017 Nov)

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR, Hynes RO
Journal of proteome research16(8)3083-3091(2017 Aug)

Comprehensive proteomic characterization of stem cell-derived extracellular matrices.
Ragelle H, Naba A, Larson BL, Zhou F, Priji? M, Whittaker CA, Del Rosario A, Langer R, Hynes RO, Anderson DG
Biomaterials128147-159(2017 06)

Keloids, Spontaneous or After Minor Skin Injury: Importance of Not Missing Bethlem Myopathy.
Echeverr?a C, Diaz A, Suarez B, Bevilacqua JA, Bonnemann C, Bertini E, Castiglioni C
Acta dermato-venereologica97(2)297-298(2017 02)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic study showed a missense mutation in COL6A2 (c.820 G>A, p.Gly268Ser) that causes a glycine substitution in the Gly-X-Y collagenous motif, at the beginning of the collagenous triple helical domain. The c.820 G>A mutation segregated in all the affected patients.
Title: Keloids, Spontaneous or After Minor Skin Injury: Importance of Not Missing Bethlem Myopathy.

Mutations in COL6A2 gene are associated with aberrant mitochondria in Bethlem myopathy.
Title: Aberrant mitochondria in a Bethlem myopathy patient with a homozygous amino acid substitution that destabilizes the collagen VI ?2(VI) chain.

Parental mosaicism was confirmed in the four families through quantitative analysis of the ratio of mutant versus wild-type allele (COL6A1, COL6A2, and COL6A3) in genomic DNA from various tissues; consistent with somatic mosaicism, parental samples had lower ratios of mutant versus wild-type allele compared with the fully heterozygote offspring.
Title: Mosaicism for dominant collagen 6 mutations as a cause for intrafamilial phenotypic variability.

In UCMD, 8 mutations were identified in COL6A2 in Chinese patients.
Title: Novel collagen VI mutations identified in Chinese patients with Ullrich congenital muscular dystrophy.

Mutations in each of the three collagen VI genes, COL6A1, COL6A2 and COL6A3, cause four types of muscle disorders: Ullrich congenital muscular dystrophy, Bethlem myopathy, limb-girdle muscular dystrophy, and autosomal recessive myosclerosis. (Review)
Title: Collagen type VI myopathies.

The following COL6A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL6A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18654	NM_001849.4 Latest version!	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu18654	XM_011529452.1 Latest version!	Homo sapiens collagen, type VI, alpha 2 (COL6A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu18654	XM_011529451.1 Latest version!	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu16184	NM_058174.3 Latest version!	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu16294	NM_058175.2 Latest version!	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2a', mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$580.30 $829.00
View Old Accession Versions >>
OHu18654	NM_001849.3	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu16184	NM_058174.2	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL6A2 ( NM_001849.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001840.3 Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2, mRNA.

Price & Availability↑

CloneID	OHu18654
Clone ID Related Accession (Same CDS sequence)	XM_011529452.1 , XM_011529451.1 , NM_001849.4 , NM_001849.3
Accession Version	NM_001849.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	collagen alpha-2(VI) chain isoform 2C2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001471.1, CN352155.1, BC065509.1, CN352095.1 and AY029208.1. On Aug 31, 2019 this sequence version replaced NM_001849.3. Transcript Variant: This variant (2C2), that differs from the other variants in the last exon, encodes the full-length protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065509.1, SRR7410570.683931.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GAGCTGTCCG TGGCACAGTG CACGCAGCGG CCCGTGGACA TCGTCTTCCT GCTGGACGGC 
TCCGAGCGGC TGGGTGAGCA GAACTTCCAC AAGGCCCGGC GCTTCGTGGA GCAGGTGGCG 
CGGCGGCTGA CGCTGGCCCG GAGGGACGAC GACCCTCTCA ACGCACGCGT GGCGCTGCTG 
CAGTTTGGTG GCCCCGGCGA GCAGCAGGTG GCCTTCCCGC TGAGCCACAA CCTCACGGCC 
ATCCACGAGG CGCTGGAGAC CACACAATAC CTGAACTCCT TCTCGCACGT GGGCGCAGGC 
GTGGTGCACG CCATCAATGC CATCGTGCGC AGCCCGCGTG GCGGGGCCCG GAGGCACGCA 
GAGCTGTCCT TCGTGTTCCT CACGGACGGC GTCACGGGCA ACGACAGTCT GCACGAGTCG 
GCGCACTCCA TGCGCAAGCA GAACGTGGTA CCCACCGTGC TGGCCTTGGG CAGCGACGTG 
GACATGGACG TGCTCACCAC GCTCAGCCTG GGTGACCGCG CCGCCGTGTT CCACGAGAAG 
GACTATGACA GCCTGGCGCA ACCCGGCTTC TTCGACCGCT TCATCCGCTG GATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001840.3
CDS	90..3149
Translation	MLQGTCSVLLLWGILGAIQAQQQEVISPDTTERNNNCPEKTDCPIHVYFVLDTSESVTMQSPTDILLFHMKQFVPQFISQLQNEFYLDQVALSWRYGGLHFSDQVEVFSPPGSDRASFIKNLQGISSFRRGTFTDCALANMTEQIRQDRSKGTVHFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVAPNQNLKEQGLRDIASTPHELYRNDYATMLPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPSGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDPGNRGPDGYPGEAGSPGERGDQGGKGDPGRPGRRGPPGEIGAKGSKGYQGNSGAPGSPGVKGAKGGPGPRGPKGEPGRRGDPGTKGSPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGEKGEPGPRGPEGGRGDFGLKGEPGRKGEKGEPADPGPPGEPGPRGPRGVPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYDRLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTELSVAQCTQRPVDIVFLLDGSERLGEQNFHKARRFVEQVARRLTLARRDDDPLNARVALLQFGGPGEQQVAFPLSHNLTAIHEALETTQYLNSFSHVGAGVVHAINAIVRSPRGGARRHAELSFVFLTDGVTGNDSLHESAHSMRKQNVVPTVLALGSDVDMDVLTTLSLGDRAAVFHEKDYDSLAQPGFFDRFIRWIC

Target ORF information:

RefSeq Version	NM_001849.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001849.4

ORF Insert Sequence:

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GAGCTGTCCG TGGCACAGTG CACGCAGCGG CCCGTGGACA TCGTCTTCCT GCTGGACGGC 
TCCGAGCGGC TGGGTGAGCA GAACTTCCAC AAGGCCCGGC GCTTCGTGGA GCAGGTGGCG 
CGGCGGCTGA CGCTGGCCCG GAGGGACGAC GACCCTCTCA ACGCACGCGT GGCGCTGCTG 
CAGTTTGGTG GCCCCGGCGA GCAGCAGGTG GCCTTCCCGC TGAGCCACAA CCTCACGGCC 
ATCCACGAGG CGCTGGAGAC CACACAATAC CTGAACTCCT TCTCGCACGT GGGCGCAGGC 
GTGGTGCACG CCATCAATGC CATCGTGCGC AGCCCGCGTG GCGGGGCCCG GAGGCACGCA 
GAGCTGTCCT TCGTGTTCCT CACGGACGGC GTCACGGGCA ACGACAGTCT GCACGAGTCG 
GCGCACTCCA TGCGCAAGCA GAACGTGGTA CCCACCGTGC TGGCCTTGGG CAGCGACGTG 
GACATGGACG TGCTCACCAC GCTCAGCCTG GGTGACCGCG CCGCCGTGTT CCACGAGAAG 
GACTATGACA GCCTGGCGCA ACCCGGCTTC TTCGACCGCT TCATCCGCTG GATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A2 ( NM_001849.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001840.3 Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2, mRNA.

Price & Availability↑

CloneID	OHu18654
Clone ID Related Accession (Same CDS sequence)	XM_011529452.1 , XM_011529451.1 , NM_001849.4 , NM_001849.3
Accession Version	NM_001849.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	collagen alpha-2(VI) chain isoform 2C2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN352155.1, BC065509.1, CN352095.1, AY029208.1 and AL044156.1. This sequence is a reference standard in the RefSeqGene project. On Oct 4, 2006 this sequence version replaced NM_001849.2. Transcript Variant: This variant (2C2), that differs from the other variants in the last exon, encodes the full-length protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GAGCTGTCCG TGGCACAGTG CACGCAGCGG CCCGTGGACA TCGTCTTCCT GCTGGACGGC 
TCCGAGCGGC TGGGTGAGCA GAACTTCCAC AAGGCCCGGC GCTTCGTGGA GCAGGTGGCG 
CGGCGGCTGA CGCTGGCCCG GAGGGACGAC GACCCTCTCA ACGCACGCGT GGCGCTGCTG 
CAGTTTGGTG GCCCCGGCGA GCAGCAGGTG GCCTTCCCGC TGAGCCACAA CCTCACGGCC 
ATCCACGAGG CGCTGGAGAC CACACAATAC CTGAACTCCT TCTCGCACGT GGGCGCAGGC 
GTGGTGCACG CCATCAATGC CATCGTGCGC AGCCCGCGTG GCGGGGCCCG GAGGCACGCA 
GAGCTGTCCT TCGTGTTCCT CACGGACGGC GTCACGGGCA ACGACAGTCT GCACGAGTCG 
GCGCACTCCA TGCGCAAGCA GAACGTGGTA CCCACCGTGC TGGCCTTGGG CAGCGACGTG 
GACATGGACG TGCTCACCAC GCTCAGCCTG GGTGACCGCG CCGCCGTGTT CCACGAGAAG 
GACTATGACA GCCTGGCGCA ACCCGGCTTC TTCGACCGCT TCATCCGCTG GATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001840.3
CDS	83..3142
Translation	MLQGTCSVLLLWGILGAIQAQQQEVISPDTTERNNNCPEKTDCPIHVYFVLDTSESVTMQSPTDILLFHMKQFVPQFISQLQNEFYLDQVALSWRYGGLHFSDQVEVFSPPGSDRASFIKNLQGISSFRRGTFTDCALANMTEQIRQDRSKGTVHFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVAPNQNLKEQGLRDIASTPHELYRNDYATMLPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPSGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDPGNRGPDGYPGEAGSPGERGDQGGKGDPGRPGRRGPPGEIGAKGSKGYQGNSGAPGSPGVKGAKGGPGPRGPKGEPGRRGDPGTKGSPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGEKGEPGPRGPEGGRGDFGLKGEPGRKGEKGEPADPGPPGEPGPRGPRGVPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYDRLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTELSVAQCTQRPVDIVFLLDGSERLGEQNFHKARRFVEQVARRLTLARRDDDPLNARVALLQFGGPGEQQVAFPLSHNLTAIHEALETTQYLNSFSHVGAGVVHAINAIVRSPRGGARRHAELSFVFLTDGVTGNDSLHESAHSMRKQNVVPTVLALGSDVDMDVLTTLSLGDRAAVFHEKDYDSLAQPGFFDRFIRWIC

Target ORF information:

RefSeq Version	NM_001849.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001849.3

ORF Insert Sequence:

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GAGCTGTCCG TGGCACAGTG CACGCAGCGG CCCGTGGACA TCGTCTTCCT GCTGGACGGC 
TCCGAGCGGC TGGGTGAGCA GAACTTCCAC AAGGCCCGGC GCTTCGTGGA GCAGGTGGCG 
CGGCGGCTGA CGCTGGCCCG GAGGGACGAC GACCCTCTCA ACGCACGCGT GGCGCTGCTG 
CAGTTTGGTG GCCCCGGCGA GCAGCAGGTG GCCTTCCCGC TGAGCCACAA CCTCACGGCC 
ATCCACGAGG CGCTGGAGAC CACACAATAC CTGAACTCCT TCTCGCACGT GGGCGCAGGC 
GTGGTGCACG CCATCAATGC CATCGTGCGC AGCCCGCGTG GCGGGGCCCG GAGGCACGCA 
GAGCTGTCCT TCGTGTTCCT CACGGACGGC GTCACGGGCA ACGACAGTCT GCACGAGTCG 
GCGCACTCCA TGCGCAAGCA GAACGTGGTA CCCACCGTGC TGGCCTTGGG CAGCGACGTG 
GACATGGACG TGCTCACCAC GCTCAGCCTG GGTGACCGCG CCGCCGTGTT CCACGAGAAG 
GACTATGACA GCCTGGCGCA ACCCGGCTTC TTCGACCGCT TCATCCGCTG GATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A2 ( XM_011529452.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527754.1 Homo sapiens collagen, type VI, alpha 2 (COL6A2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu18654
Clone ID Related Accession (Same CDS sequence)	XM_011529452.1 , XM_011529451.1 , NM_001849.4 , NM_001849.3
Accession Version	XM_011529452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	collagen alpha-2(VI) chain isoform X1
Comment

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GAGCTGTCCG TGGCACAGTG CACGCAGCGG CCCGTGGACA TCGTCTTCCT GCTGGACGGC 
TCCGAGCGGC TGGGTGAGCA GAACTTCCAC AAGGCCCGGC GCTTCGTGGA GCAGGTGGCG 
CGGCGGCTGA CGCTGGCCCG GAGGGACGAC GACCCTCTCA ACGCACGCGT GGCGCTGCTG 
CAGTTTGGTG GCCCCGGCGA GCAGCAGGTG GCCTTCCCGC TGAGCCACAA CCTCACGGCC 
ATCCACGAGG CGCTGGAGAC CACACAATAC CTGAACTCCT TCTCGCACGT GGGCGCAGGC 
GTGGTGCACG CCATCAATGC CATCGTGCGC AGCCCGCGTG GCGGGGCCCG GAGGCACGCA 
GAGCTGTCCT TCGTGTTCCT CACGGACGGC GTCACGGGCA ACGACAGTCT GCACGAGTCG 
GCGCACTCCA TGCGCAAGCA GAACGTGGTA CCCACCGTGC TGGCCTTGGG CAGCGACGTG 
GACATGGACG TGCTCACCAC GCTCAGCCTG GGTGACCGCG CCGCCGTGTT CCACGAGAAG 
GACTATGACA GCCTGGCGCA ACCCGGCTTC TTCGACCGCT TCATCCGCTG GATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011527754.1

Translation

MLQGTCSVLLLWGILGAIQAQQQEVISPDTTERNNNCPEKTDCPIHVYFVLDTSESVTMQSPTDILLFHMKQFVPQFISQLQNEFYLDQVALSWRYGGLHFSDQVEVFSPPGSDRASFIKNLQGISSFRRGTFTDCALANMTEQIRQDRSKGTVHFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVAPNQNLKEQGLRDIASTPHELYRNDYATMLPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPSGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDPGNRGPDGYPGEAGSPGERGDQGGKGDPGRPGRRGPPGEIGAKGSKGYQGNSGAPGSPGVKGAKGGPGPRGPKGEPGRRGDPGTKGSPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGEKGEPGPRGPEGGRGDFGLKGEPGRKGEKGEPADPGPPGEPGPRGPRGVPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYDRLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTELSVAQCTQRPVDIVFLLDGSERLGEQNFHKARRFVEQVARRLTLARRDDDPLNARVALLQFGGPGEQQVAFPLSHNLTAIHEALETTQYLNSFSHVGAGVVHAINAIVRSPRGGARRHAELSFVFLTDGVTGNDSLHESAHSMRKQNVVPTVLALGSDVDMDVLTTLSLGDRAAVFHEKDYDSLAQPGFFDRFIRWIC

Target ORF information:

RefSeq Version	XM_011529452.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens collagen, type VI, alpha 2 (COL6A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529452.1

ORF Insert Sequence:

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GAGCTGTCCG TGGCACAGTG CACGCAGCGG CCCGTGGACA TCGTCTTCCT GCTGGACGGC 
TCCGAGCGGC TGGGTGAGCA GAACTTCCAC AAGGCCCGGC GCTTCGTGGA GCAGGTGGCG 
CGGCGGCTGA CGCTGGCCCG GAGGGACGAC GACCCTCTCA ACGCACGCGT GGCGCTGCTG 
CAGTTTGGTG GCCCCGGCGA GCAGCAGGTG GCCTTCCCGC TGAGCCACAA CCTCACGGCC 
ATCCACGAGG CGCTGGAGAC CACACAATAC CTGAACTCCT TCTCGCACGT GGGCGCAGGC 
GTGGTGCACG CCATCAATGC CATCGTGCGC AGCCCGCGTG GCGGGGCCCG GAGGCACGCA 
GAGCTGTCCT TCGTGTTCCT CACGGACGGC GTCACGGGCA ACGACAGTCT GCACGAGTCG 
GCGCACTCCA TGCGCAAGCA GAACGTGGTA CCCACCGTGC TGGCCTTGGG CAGCGACGTG 
GACATGGACG TGCTCACCAC GCTCAGCCTG GGTGACCGCG CCGCCGTGTT CCACGAGAAG 
GACTATGACA GCCTGGCGCA ACCCGGCTTC TTCGACCGCT TCATCCGCTG GATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A2 ( XM_011529451.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527753.1 Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu18654
Clone ID Related Accession (Same CDS sequence)	XM_011529452.1 , XM_011529451.1 , NM_001849.4 , NM_001849.3
Accession Version	XM_011529451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-2(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GAGCTGTCCG TGGCACAGTG CACGCAGCGG CCCGTGGACA TCGTCTTCCT GCTGGACGGC 
TCCGAGCGGC TGGGTGAGCA GAACTTCCAC AAGGCCCGGC GCTTCGTGGA GCAGGTGGCG 
CGGCGGCTGA CGCTGGCCCG GAGGGACGAC GACCCTCTCA ACGCACGCGT GGCGCTGCTG 
CAGTTTGGTG GCCCCGGCGA GCAGCAGGTG GCCTTCCCGC TGAGCCACAA CCTCACGGCC 
ATCCACGAGG CGCTGGAGAC CACACAATAC CTGAACTCCT TCTCGCACGT GGGCGCAGGC 
GTGGTGCACG CCATCAATGC CATCGTGCGC AGCCCGCGTG GCGGGGCCCG GAGGCACGCA 
GAGCTGTCCT TCGTGTTCCT CACGGACGGC GTCACGGGCA ACGACAGTCT GCACGAGTCG 
GCGCACTCCA TGCGCAAGCA GAACGTGGTA CCCACCGTGC TGGCCTTGGG CAGCGACGTG 
GACATGGACG TGCTCACCAC GCTCAGCCTG GGTGACCGCG CCGCCGTGTT CCACGAGAAG 
GACTATGACA GCCTGGCGCA ACCCGGCTTC TTCGACCGCT TCATCCGCTG GATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527753.1
CDS	374..3433
Translation	MLQGTCSVLLLWGILGAIQAQQQEVISPDTTERNNNCPEKTDCPIHVYFVLDTSESVTMQSPTDILLFHMKQFVPQFISQLQNEFYLDQVALSWRYGGLHFSDQVEVFSPPGSDRASFIKNLQGISSFRRGTFTDCALANMTEQIRQDRSKGTVHFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVAPNQNLKEQGLRDIASTPHELYRNDYATMLPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPSGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDPGNRGPDGYPGEAGSPGERGDQGGKGDPGRPGRRGPPGEIGAKGSKGYQGNSGAPGSPGVKGAKGGPGPRGPKGEPGRRGDPGTKGSPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGEKGEPGPRGPEGGRGDFGLKGEPGRKGEKGEPADPGPPGEPGPRGPRGVPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYDRLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTELSVAQCTQRPVDIVFLLDGSERLGEQNFHKARRFVEQVARRLTLARRDDDPLNARVALLQFGGPGEQQVAFPLSHNLTAIHEALETTQYLNSFSHVGAGVVHAINAIVRSPRGGARRHAELSFVFLTDGVTGNDSLHESAHSMRKQNVVPTVLALGSDVDMDVLTTLSLGDRAAVFHEKDYDSLAQPGFFDRFIRWIC

Target ORF information:

RefSeq Version	XM_011529451.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529451.1

ORF Insert Sequence:

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GAGCTGTCCG TGGCACAGTG CACGCAGCGG CCCGTGGACA TCGTCTTCCT GCTGGACGGC 
TCCGAGCGGC TGGGTGAGCA GAACTTCCAC AAGGCCCGGC GCTTCGTGGA GCAGGTGGCG 
CGGCGGCTGA CGCTGGCCCG GAGGGACGAC GACCCTCTCA ACGCACGCGT GGCGCTGCTG 
CAGTTTGGTG GCCCCGGCGA GCAGCAGGTG GCCTTCCCGC TGAGCCACAA CCTCACGGCC 
ATCCACGAGG CGCTGGAGAC CACACAATAC CTGAACTCCT TCTCGCACGT GGGCGCAGGC 
GTGGTGCACG CCATCAATGC CATCGTGCGC AGCCCGCGTG GCGGGGCCCG GAGGCACGCA 
GAGCTGTCCT TCGTGTTCCT CACGGACGGC GTCACGGGCA ACGACAGTCT GCACGAGTCG 
GCGCACTCCA TGCGCAAGCA GAACGTGGTA CCCACCGTGC TGGCCTTGGG CAGCGACGTG 
GACATGGACG TGCTCACCAC GCTCAGCCTG GGTGACCGCG CCGCCGTGTT CCACGAGAAG 
GACTATGACA GCCTGGCGCA ACCCGGCTTC TTCGACCGCT TCATCCGCTG GATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A2 ( NM_058174.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_478054.2 Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2a, mRNA.

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CloneID	OHu16184
Clone ID Related Accession (Same CDS sequence)	NM_058174.3 , NM_058174.2
Accession Version	NM_058174.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	collagen alpha-2(VI) chain isoform 2C2a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001471.1, CN352155.1, BC065509.1, CN352095.1, X15977.1, BM989656.1 and AI275476.1. On Jun 2, 2019 this sequence version replaced NM_058174.2. Transcript Variant: This variant (2C2a) has a different last exon than the other variants. Isoform 2C2a is 101 amino acids shorter than isoform 2C2 and 91 amino acids longer than isoform 2C2a'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1803616.162961.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GATGCACCGT GGCCTGGCGG CGAGCCCCCG GTCACCTTCC TCCGCACGGA AGAGGGGCCG 
GACGCCACCT TCCCCAGGAC CATTCCCCTG ATCCAACAGT TGCTAAACGC CACGGAGCTC 
ACGCAGGACC CGGCCGCCTA CTCCCAGCTG GTGGCCGTGC TGGTCTACAC CGCCGAGCGG 
GCCAAGTTCG CCACCGGGGT AGAGCGGCAG GACTGGATGG AGCTGTTCAT TGACACCTTT 
AAGCTGGTGC ACAGGGACAT CGTGGGGGAC CCCGAGACCG CGCTGGCCCT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_478054.2
CDS	90..2846
Translation	MLQGTCSVLLLWGILGAIQAQQQEVISPDTTERNNNCPEKTDCPIHVYFVLDTSESVTMQSPTDILLFHMKQFVPQFISQLQNEFYLDQVALSWRYGGLHFSDQVEVFSPPGSDRASFIKNLQGISSFRRGTFTDCALANMTEQIRQDRSKGTVHFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVAPNQNLKEQGLRDIASTPHELYRNDYATMLPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPSGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDPGNRGPDGYPGEAGSPGERGDQGGKGDPGRPGRRGPPGEIGAKGSKGYQGNSGAPGSPGVKGAKGGPGPRGPKGEPGRRGDPGTKGSPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGEKGEPGPRGPEGGRGDFGLKGEPGRKGEKGEPADPGPPGEPGPRGPRGVPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYDRLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTDAPWPGGEPPVTFLRTEEGPDATFPRTIPLIQQLLNATELTQDPAAYSQLVAVLVYTAERAKFATGVERQDWMELFIDTFKLVHRDIVGDPETALALC

Target ORF information:

RefSeq Version	NM_058174.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058174.3

ORF Insert Sequence:

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GATGCACCGT GGCCTGGCGG CGAGCCCCCG GTCACCTTCC TCCGCACGGA AGAGGGGCCG 
GACGCCACCT TCCCCAGGAC CATTCCCCTG ATCCAACAGT TGCTAAACGC CACGGAGCTC 
ACGCAGGACC CGGCCGCCTA CTCCCAGCTG GTGGCCGTGC TGGTCTACAC CGCCGAGCGG 
GCCAAGTTCG CCACCGGGGT AGAGCGGCAG GACTGGATGG AGCTGTTCAT TGACACCTTT 
AAGCTGGTGC ACAGGGACAT CGTGGGGGAC CCCGAGACCG CGCTGGCCCT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A2 ( NM_058174.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_478054.2 Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2a, mRNA.

Price & Availability↑

CloneID	OHu16184
Clone ID Related Accession (Same CDS sequence)	NM_058174.3 , NM_058174.2
Accession Version	NM_058174.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	collagen alpha-2(VI) chain isoform 2C2a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN352155.1, BC065509.1, CN352095.1, X15977.1, BM989656.1 and AI275476.1. On Oct 4, 2006 this sequence version replaced NM_058174.1. Transcript Variant: This variant (2C2a) has a different last exon than the other variants. Isoform 2C2a is 101 amino acids shorter than isoform 2C2 and 91 amino acids longer than isoform 2C2a'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GATGCACCGT GGCCTGGCGG CGAGCCCCCG GTCACCTTCC TCCGCACGGA AGAGGGGCCG 
GACGCCACCT TCCCCAGGAC CATTCCCCTG ATCCAACAGT TGCTAAACGC CACGGAGCTC 
ACGCAGGACC CGGCCGCCTA CTCCCAGCTG GTGGCCGTGC TGGTCTACAC CGCCGAGCGG 
GCCAAGTTCG CCACCGGGGT AGAGCGGCAG GACTGGATGG AGCTGTTCAT TGACACCTTT 
AAGCTGGTGC ACAGGGACAT CGTGGGGGAC CCCGAGACCG CGCTGGCCCT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_478054.2
CDS	83..2839
Translation	MLQGTCSVLLLWGILGAIQAQQQEVISPDTTERNNNCPEKTDCPIHVYFVLDTSESVTMQSPTDILLFHMKQFVPQFISQLQNEFYLDQVALSWRYGGLHFSDQVEVFSPPGSDRASFIKNLQGISSFRRGTFTDCALANMTEQIRQDRSKGTVHFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVAPNQNLKEQGLRDIASTPHELYRNDYATMLPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPSGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDPGNRGPDGYPGEAGSPGERGDQGGKGDPGRPGRRGPPGEIGAKGSKGYQGNSGAPGSPGVKGAKGGPGPRGPKGEPGRRGDPGTKGSPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGEKGEPGPRGPEGGRGDFGLKGEPGRKGEKGEPADPGPPGEPGPRGPRGVPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYDRLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTDAPWPGGEPPVTFLRTEEGPDATFPRTIPLIQQLLNATELTQDPAAYSQLVAVLVYTAERAKFATGVERQDWMELFIDTFKLVHRDIVGDPETALALC

Target ORF information:

RefSeq Version	NM_058174.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058174.2

ORF Insert Sequence:

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GATGCACCGT GGCCTGGCGG CGAGCCCCCG GTCACCTTCC TCCGCACGGA AGAGGGGCCG 
GACGCCACCT TCCCCAGGAC CATTCCCCTG ATCCAACAGT TGCTAAACGC CACGGAGCTC 
ACGCAGGACC CGGCCGCCTA CTCCCAGCTG GTGGCCGTGC TGGTCTACAC CGCCGAGCGG 
GCCAAGTTCG CCACCGGGGT AGAGCGGCAG GACTGGATGG AGCTGTTCAT TGACACCTTT 
AAGCTGGTGC ACAGGGACAT CGTGGGGGAC CCCGAGACCG CGCTGGCCCT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A2 ( NM_058175.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_478055.2 Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2a', mRNA.

Price & Availability↑

CloneID	OHu16294
Clone ID Related Accession (Same CDS sequence)	NM_058175.2
Accession Version	NM_058175.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Homo sapiens(human)
Product	collagen alpha-2(VI) chain isoform 2C2a' precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN352155.1, BC065509.1, CN352095.1, M34570.1, BM989656.1 and AI275476.1. On Oct 4, 2006 this sequence version replaced NM_058175.1. Transcript Variant: This variant (2C2a') encodes the shortest polypeptide. The last exon in this variant results in the use of a stop codon that shortens the protein by 192 amino acids compared to the full-length protein, although the transcripts are nearly the same length. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GGTTTGGACG GAGCTGTTTT GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_478055.2
CDS	83..2569
Translation	MLQGTCSVLLLWGILGAIQAQQQEVISPDTTERNNNCPEKTDCPIHVYFVLDTSESVTMQSPTDILLFHMKQFVPQFISQLQNEFYLDQVALSWRYGGLHFSDQVEVFSPPGSDRASFIKNLQGISSFRRGTFTDCALANMTEQIRQDRSKGTVHFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVAPNQNLKEQGLRDIASTPHELYRNDYATMLPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPSGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDPGNRGPDGYPGEAGSPGERGDQGGKGDPGRPGRRGPPGEIGAKGSKGYQGNSGAPGSPGVKGAKGGPGPRGPKGEPGRRGDPGTKGSPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGEKGEPGPRGPEGGRGDFGLKGEPGRKGEKGEPADPGPPGEPGPRGPRGVPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYDRLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTGLDGAVLC

Target ORF information:

RefSeq Version	NM_058175.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript variant 2C2a', mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058175.2

ORF Insert Sequence:

ATGCTCCAGG GCACCTGCTC CGTGCTCCTG CTCTGGGGAA TCCTGGGGGC CATCCAGGCC 
CAGCAGCAGG AGGTCATCTC GCCGGACACT ACCGAGAGAA ACAACAACTG CCCAGAGAAG 
ACCGACTGCC CCATCCACGT GTACTTCGTG CTGGACACCT CGGAGAGCGT CACCATGCAG 
TCCCCCACGG ACATCCTGCT CTTCCACATG AAGCAGTTCG TGCCGCAGTT CATCAGCCAG 
CTGCAGAACG AGTTCTACCT GGACCAGGTG GCGCTGAGCT GGCGCTACGG CGGCCTGCAC 
TTCTCTGACC AGGTGGAGGT GTTCAGCCCA CCGGGCAGCG ACCGGGCCTC CTTCATCAAG 
AACCTGCAGG GCATCAGCTC CTTCCGCCGC GGCACCTTCA CCGACTGCGC GCTGGCCAAC 
ATGACGGAGC AGATCCGGCA GGACCGCAGC AAGGGCACCG TCCACTTCGC CGTGGTCATC 
ACCGACGGCC ACGTCACCGG CAGCCCCTGC GGGGGCATCA AGCTGCAGGC CGAGCGGGCC 
CGCGAGGAGG GCATCCGGCT CTTCGCCGTG GCCCCCAACC AGAACCTGAA GGAGCAGGGC 
CTGCGGGACA TCGCCAGCAC GCCGCACGAG CTCTACCGCA ACGACTACGC CACCATGCTG 
CCCGACTCCA CCGAGATCGA CCAGGACACC ATCAACCGCA TCATCAAGGT CATGAAACAC 
GAAGCCTACG GAGAGTGCTA CAAGGTGAGC TGCCTGGAAA TCCCTGGGCC CTCTGGCCCC 
AAGGGCTACC GTGGACAGAA GGGTGCCAAG GGCAACATGG GTGAGCCGGG AGAGCCTGGC 
CAGAAGGGAA GACAGGGAGA CCCGGGCATC GAAGGCCCCA TTGGATTCCC AGGACCCAAG 
GGCGTTCCTG GCTTCAAAGG AGAGAAGGGT GAATTTGGAG CCGACGGTCG CAAGGGGGCC 
CCTGGCCTGG CTGGCAAGAA CGGGACCGAT GGACAGAAGG GCAAGCTGGG GCGCATCGGA 
CCTCCTGGCT GCAAGGGAGA CCCTGGAAAC CGGGGCCCCG ACGGTTACCC GGGGGAAGCA 
GGGAGTCCAG GGGAGCGAGG AGACCAAGGC GGCAAGGGGG ACCCTGGCCG CCCAGGACGC 
AGAGGGCCCC CGGGAGAAAT CGGGGCCAAG GGAAGCAAGG GGTATCAAGG CAACAGTGGA 
GCCCCAGGAA GTCCTGGTGT GAAAGGAGCC AAGGGCGGGC CTGGGCCCCG CGGACCCAAA 
GGCGAGCCGG GGCGCAGGGG AGACCCCGGC ACCAAGGGCA GCCCAGGCAG CGATGGCCCC 
AAGGGGGAGA AGGGGGACCC TGGCCCTGAG GGGCCCCGCG GCCTGGCTGG AGAGGTTGGC 
AACAAAGGAG CCAAGGGAGA CCGAGGCTTG CCTGGACCCA GAGGCCCCCA GGGAGCTCTT 
GGGGAGCCCG GAAAGCAGGG ATCTCGGGGA GACCCCGGTG ATGCAGGACC CCGTGGAGAC 
TCAGGACAGC CAGGCCCCAA GGGAGACCCC GGCAGGCCTG GATTCAGCTA CCCAGGACCC 
CGAGGAGCAC CCGGAGAAAA AGGCGAGCCC GGCCCACGCG GCCCCGAGGG AGGCCGAGGC 
GACTTTGGCT TGAAAGGAGA ACCTGGGAGG AAAGGAGAGA AAGGAGAGCC TGCGGATCCT 
GGTCCCCCTG GTGAGCCAGG CCCTCGGGGG CCAAGAGGAG TCCCAGGACC CGAGGGTGAG 
CCCGGCCCCC CTGGAGACCC CGGTCTCACG GAGTGTGACG TCATGACCTA CGTGAGGGAG 
ACCTGCGGGT GCTGCGACTG TGAGAAGCGC TGTGGCGCCC TGGACGTGGT CTTCGTCATC 
GACAGCTCCG AGAGCATTGG GTACACCAAC TTCACACTGG AGAAGAACTT CGTCATCAAC 
GTGGTCAACA GGCTGGGTGC CATCGCTAAG GACCCCAAGT CCGAGACAGG GACGCGTGTG 
GGCGTGGTGC AGTACAGCCA CGAGGGCACC TTTGAGGCCA TCCAGCTGGA CGACGAACGT 
ATCGACTCCC TGTCGAGCTT CAAGGAGGCT GTCAAGAACC TCGAGTGGAT TGCGGGCGGC 
ACCTGGACAC CCTCAGCCCT CAAGTTTGCC TACGACCGCC TCATCAAGGA GAGCCGGCGC 
CAGAAGACAC GTGTGTTTGC GGTGGTCATC ACGGACGGGC GCCACGACCC TCGGGACGAT 
GACCTCAACT TGCGGGCGCT GTGCGACCGC GACGTCACAG TGACGGCCAT CGGCATCGGG 
GACATGTTCC ACGAGAAGCA CGAGAGTGAA AACCTCTACT CCATCGCCTG CGACAAGCCA 
CAGCAGGTGC GCAACATGAC GCTGTTCTCC GACCTGGTCG CTGAGAAGTT CATCGATGAC 
ATGGAGGACG TCCTCTGCCC GGACCCTCAG ATCGTGTGCC CAGACCTTCC CTGCCAAACA 
GGTTTGGACG GAGCTGTTTT GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic characterization of human multiple myeloma bone marrow extracellular matrix.
Leukemia31(11)2426-2434(2017 Nov)
Glavey SV,Naba A,Manier S,Clauser K,Tahri S,Park J,Reagan MR,Moschetta M,Mishima Y,Gambella M,Rocci A,Sacco A,O'Dwyer ME,Asara JM,Palumbo A,Roccaro AM,Hynes RO,Ghobrial IM

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Journal of proteome research16(8)3083-3091(2017 Aug)
Naba A,Pearce OMT,Del Rosario A,Ma D,Ding H,Rajeeve V,Cutillas PR,Balkwill FR,Hynes RO

Comprehensive proteomic characterization of stem cell-derived extracellular matrices.
Biomaterials128147-159(2017 06)
Ragelle H,Naba A,Larson BL,Zhou F,Priji? M,Whittaker CA,Del Rosario A,Langer R,Hynes RO,Anderson DG

Keloids, Spontaneous or After Minor Skin Injury: Importance of Not Missing Bethlem Myopathy.
Acta dermato-venereologica97(2)297-298(2017 02)
Echeverr?a C,Diaz A,Suarez B,Bevilacqua JA,Bonnemann C,Bertini E,Castiglioni C

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COL6A2 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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