col6a2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	col6a2
Entrez Gene ID	567771
Full Name	collagen, type VI, alpha 2
General protein information	Preferred Names collagen, type VI, alpha 2 Names collagen alpha-2(VI) chain
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021479873.1	XP_021335548.1	collagen alpha-2(VI) chain isoform X3
XM_691072.8	XP_696164.2	collagen alpha-2(VI) chain isoform X4
XM_009306168.3	XP_009304443.2	collagen alpha-2(VI) chain isoform X2
XM_009306168.2	XP_009304443.2	collagen alpha-2(VI) chain isoform X1
XM_691072.7	XP_696164.2	collagen alpha-2(VI) chain isoform X2
XM_021479872.1	XP_021335547.1	collagen alpha-2(VI) chain isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL6A2	XP_531504.3
Gallus gallus (chicken)	COL6A2	NP_990679.1
Homo sapiens (human)	COL6A2	NP_001840.3
Mus musculus (house mouse)	Col6a2	NP_666119.1
Xenopus tropicalis (tropical clawed frog)	LOC100145523	NP_001120436.1
Danio rerio (zebrafish)	col6a2	XP_696164.2
	COL6A2	XP_001099130.2
Rattus norvegicus (Norway rat)	Col6a2	NP_001094211.1

Gene Ontology

The following col6a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col6a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148036	XM_021479872.1 Latest version!	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa148037	XM_021479873.1 Latest version!	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa17233	XM_691072.8 Latest version!	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa54364	XM_009306168.3 Latest version!	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
ODa54364	XM_009306168.2	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa17233	XM_691072.7	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col6a2 ( XM_021479872.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335547.1 Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa148036
Clone ID Related Accession (Same CDS sequence)	XM_021479872.1
Accession Version	XM_021479872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGG ATCCAGACTT CTGTTCAAGC TGGGAGAAGC GCAGAGGAAA CCTCTCCTGC 
AGCTTGGCTT CTCACTCACT CCCTCATTTT GGACTTTATT CTGATTTCTG CTTCTACCTG 
GTTCGGAATG TATGCTTGGT AGTCCTCAAC ATAAGTCATC AGTCGAGAAG TTCTTTAGAT 
TCTCTGGTCT TGGGAAGGAG AGTGAGGATG ATGAGCTTGT CGATCCTCCT CCTGTTGTTT 
GTGGGTGTTC ATGCACAGGG CCAGACTCCA GCCGGCTCTT CGTGTAGTCC TATAATGTGT 
TCTGTCCCTT CAGAGAGTAC CGAATGCCCG ATCGACTTGT ACTTTGCTAT TGACACATCT 
GAGACCATCG CTCTTCAGGA GAATCCGCCA GGCAGCTTGG TAGAGAGTAT CAAAGATTTC 
ACCATCAGAT TTGCTGAGAA GCTCCAAAAT GTGAACTACA GAGGATCCGT GAACATCACC 
TGGGCCATCG GAGGACTCCA CTTCTCCCAG CTCCAGGAGT TCTTCAGCAC CATCACCACT 
AAAGAGAAGT TCATCAGTAA TCTCCGGCCC ATCAGATACC TAGGCAGGGG CACGCACATT 
GATTGCGCCA TCACCAACAT GACCAAGCAG CTGGTCCGCT TTCCATCCAG ACCCGATGCC 
AAACGATTCG CCGTGGTCAT CACTGATGGT CACGTGACTG CGAACCCATG CGGAGGGATC 
AAAGTGGCGG CAGAACGGGC ACGCGATGAA AATATTAGGA TCTTCGCTGT GGCATCGTCC 
AGGAATTTGG AGGAGACGGG ACTCAGGGAG ATAGCAAACT CACCCGCTGG AGTTTACCGG 
GATAAATACA TGGCGGTGAA TCTCACTGGA ACACGGCCGG TCATTGTGAC GTCCACAATC 
GATCGCATTT ATGACACAAT GCTTCATATG GCTTACCAAG AGTGCTACTC TACCAAATGC 
TTGGAGACTC CAGGACCTCC AGGTCCCCCA GGACACAGAG GACAGAAAGG AGCTAAAGGA 
GATAATGGGG GCGCTGGCCT TAAAGGTGAA AGGGGACGAC CTGGAGATCC TGGTATTGAA 
GGACCCATTG GACATCCTGG TCCTAAAGGA GAGCCTGGTC TCAAAGGAGA AAAGGGAGAA 
ATTGGAGCAC AAGGAAAAAA GGGTGTTGCA GGAATAGCTG GCCGTGATGG AAAAGATGGA 
CAGAAGGGAA AATTTGGCAG GATTGGTGCT CCTGGCTGCA AAGGTGATCC AGGTGACAAG 
GGTCCTGATG GTCATCCTGG AGATGTTGGA GAGCCTGGTC TTCCAGGCGC TTTGGGAGAT 
AAGGGTGATC GAGGTCGTCC AGGAAGATCA GGTCCTCCAG GCCCAGCTGG AGATCCTGGA 
CCAAAAGGAG AGAGAGGAAG TCCTGGATCG CCTGGAATTC CTGGCACAAA AGGAGGCGCT 
GGGACAGCTG GAGGACCTGG ACCCAAAGGA GATGCTGGGC GAAGAGGAGA TTTTGGACCT 
AAAGGAAGCA CTGGACCTAA TGGGGCAAAA GGAGAGAAGG GTGAACCAGG TGCTGAGGGA 
CCCAGAGGTC TTGCAGGTGA AGTTGGCGGC AAAGGATCCA AGGGTGATAA CGGACTTCCA 
GGACCAAGAG GCCCACCAGG AGCTCCTGGA GATGCAGGCA GAAAGGGTTC ATCAGGTGAC 
CCAGGTGATT CTGGCCCTAG AGGTGATCCT GGCCCTCCAG GACCAAAGGG TGATTCTGGC 
AGACCTGGAT TCAGCTATCC TGGACCAAGA GGACCAACGG GTGACAGGGG TGAAAAAGGT 
TCTCCTGGAC CCAGGGGAAG CAGAGGAGAC TGTGGGCCAA AGGGTGACCC AGGTCTAAAG 
GGAACTCCAG GAGAGCCGGG AGAACCTGGC CCAGCAGGAG AGCCTGGACA GAGGGGTCCT 
AGAGGAGATG GAGGAAGAGA TGGTGATCCT GGACCAGAGG GAGATCCTGG CCTTACTGAA 
TGTGATGTCA TGAACTACAT CAGAGAAACC TGTGGATGCT GTGATTGTGA AAAGAGATGC 
GGTGCCCTGG ATATTGTGTT TGTGATTGAC AGCTCTGAGA GTGTGGGGCT GACCAACTTC 
ACCCTGGAGA AGAATTTTGT GATCAACACC ATTAACAGAC TGGGCTCAAT CGCTAAAGAT 
CCCAGCTCTG AAACAGGAAC TCGAGTTGGT GTCGTCCAGT ACAGTCACAA CGGAACATTC 
CAGGCCATTC GACTCAACGA CTCCAAGATC GACTCCATGT CAGCCTTCAA AGATGCTGTG 
AAGAAGCTAG AGTGGATTGC TGGAGGAACG TGGACTCCTT CAGCCCTGAA GTTCGCCTAC 
GATAACCTGA TTCGTGACAG CCGTCGGTCA AAAGCCAATG TGACGGTGGT CGTAATCACA 
GATGGTCGGT ATGATCCCCG GGATGATGAC AAACTGCTCA ATTACCTCTG CACCGACACC 
AGCATTGATG TCAACGCTAT TGGTATTGGA GATATGTTTG ACCAGCCGGA GGAAAACGAA 
AGCTTGAAGT CTATCGCCTG TAGAAAAGAT GGAAGAGTCA TGGGAATGAG GCGTTTCGCT 
GATCTGGTCG CAGAAGATTT CATCGACAGG ATTGAGACTG TGCTTTGTCC GGATCCTGTG 
GTCATTTGCC CTGATCTTCC ATGTAAAAGT GAGCCAGCCG TGGCCAACTG CATCCAGCGG 
CCTGTTGATC TGGTCTTCAT GCTGGACGGC TCAGAGCGTA TGGGCCAGGA GAATTTCCGC 
TATGCACGTG AATTCGTGGA GAATGTGGCC AACCGTCTCA CACTAGCTCA GGGAGATGAT 
GATGAACGCA ATGCAAGGGT GGCTCTCCTG CAATATGGAG ATGAAAACCA ACATCAGTTG 
GCCTTCAAAC TAACAAATAA CTTCACCGTC ATCGCTGATG GCCTGGCCAA CATGAGGTAC 
CTAGATTCAA CCTCCAATGT TGGAAGTGGC ATCATCTACG CCATCAACAA TATCGTCACA 
AGCAGAGGCA CTCGATTGGC AAGACGAAAT GCAGAGTTGT CGTTTGTCTT CATTACCGAT 
GGGGTCACCA GTAACAAAAA CCTGGAAGAG GGGATTAGTG CCATGCGCAG AGCCGAGGGC 
GTTCCCACTG TCATTGCCAT GAGCAATGAT GTTGACAAGG AAATCCTTGC AAAGATTTCC 
CTGGGAGACC AGACGGCCGT TTTCAGGGGT GAAGATTTTG CCCGTCTGAA CAAAGCCAGC 
TTTTTTGAGC GATTCGTTCG GTGGATCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335547.1
CDS	103..3375
Translation	MKEDPDFCSSWEKRRGNLSCSLASHSLPHFGLYSDFCFYLVRNVCLVVLNISHQSRSSLDSLVLGRRVRMMSLSILLLLFVGVHAQGQTPAGSSCSPIMCSVPSESTECPIDLYFAIDTSETIALQENPPGSLVESIKDFTIRFAEKLQNVNYRGSVNITWAIGGLHFSQLQEFFSTITTKEKFISNLRPIRYLGRGTHIDCAITNMTKQLVRFPSRPDAKRFAVVITDGHVTANPCGGIKVAAERARDENIRIFAVASSRNLEETGLREIANSPAGVYRDKYMAVNLTGTRPVIVTSTIDRIYDTMLHMAYQECYSTKCLETPGPPGPPGHRGQKGAKGDNGGAGLKGERGRPGDPGIEGPIGHPGPKGEPGLKGEKGEIGAQGKKGVAGIAGRDGKDGQKGKFGRIGAPGCKGDPGDKGPDGHPGDVGEPGLPGALGDKGDRGRPGRSGPPGPAGDPGPKGERGSPGSPGIPGTKGGAGTAGGPGPKGDAGRRGDFGPKGSTGPNGAKGEKGEPGAEGPRGLAGEVGGKGSKGDNGLPGPRGPPGAPGDAGRKGSSGDPGDSGPRGDPGPPGPKGDSGRPGFSYPGPRGPTGDRGEKGSPGPRGSRGDCGPKGDPGLKGTPGEPGEPGPAGEPGQRGPRGDGGRDGDPGPEGDPGLTECDVMNYIRETCGCCDCEKRCGALDIVFVIDSSESVGLTNFTLEKNFVINTINRLGSIAKDPSSETGTRVGVVQYSHNGTFQAIRLNDSKIDSMSAFKDAVKKLEWIAGGTWTPSALKFAYDNLIRDSRRSKANVTVVVITDGRYDPRDDDKLLNYLCTDTSIDVNAIGIGDMFDQPEENESLKSIACRKDGRVMGMRRFADLVAEDFIDRIETVLCPDPVVICPDLPCKSEPAVANCIQRPVDLVFMLDGSERMGQENFRYAREFVENVANRLTLAQGDDDERNARVALLQYGDENQHQLAFKLTNNFTVIADGLANMRYLDSTSNVGSGIIYAINNIVTSRGTRLARRNAELSFVFITDGVTSNKNLEEGISAMRRAEGVPTVIAMSNDVDKEILAKISLGDQTAVFRGEDFARLNKASFFERFVRWIC

Target ORF information:

RefSeq Version	XM_021479872.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479872.1

ORF Insert Sequence:

ATGAAGGAGG ATCCAGACTT CTGTTCAAGC TGGGAGAAGC GCAGAGGAAA CCTCTCCTGC 
AGCTTGGCTT CTCACTCACT CCCTCATTTT GGACTTTATT CTGATTTCTG CTTCTACCTG 
GTTCGGAATG TATGCTTGGT AGTCCTCAAC ATAAGTCATC AGTCGAGAAG TTCTTTAGAT 
TCTCTGGTCT TGGGAAGGAG AGTGAGGATG ATGAGCTTGT CGATCCTCCT CCTGTTGTTT 
GTGGGTGTTC ATGCACAGGG CCAGACTCCA GCCGGCTCTT CGTGTAGTCC TATAATGTGT 
TCTGTCCCTT CAGAGAGTAC CGAATGCCCG ATCGACTTGT ACTTTGCTAT TGACACATCT 
GAGACCATCG CTCTTCAGGA GAATCCGCCA GGCAGCTTGG TAGAGAGTAT CAAAGATTTC 
ACCATCAGAT TTGCTGAGAA GCTCCAAAAT GTGAACTACA GAGGATCCGT GAACATCACC 
TGGGCCATCG GAGGACTCCA CTTCTCCCAG CTCCAGGAGT TCTTCAGCAC CATCACCACT 
AAAGAGAAGT TCATCAGTAA TCTCCGGCCC ATCAGATACC TAGGCAGGGG CACGCACATT 
GATTGCGCCA TCACCAACAT GACCAAGCAG CTGGTCCGCT TTCCATCCAG ACCCGATGCC 
AAACGATTCG CCGTGGTCAT CACTGATGGT CACGTGACTG CGAACCCATG CGGAGGGATC 
AAAGTGGCGG CAGAACGGGC ACGCGATGAA AATATTAGGA TCTTCGCTGT GGCATCGTCC 
AGGAATTTGG AGGAGACGGG ACTCAGGGAG ATAGCAAACT CACCCGCTGG AGTTTACCGG 
GATAAATACA TGGCGGTGAA TCTCACTGGA ACACGGCCGG TCATTGTGAC GTCCACAATC 
GATCGCATTT ATGACACAAT GCTTCATATG GCTTACCAAG AGTGCTACTC TACCAAATGC 
TTGGAGACTC CAGGACCTCC AGGTCCCCCA GGACACAGAG GACAGAAAGG AGCTAAAGGA 
GATAATGGGG GCGCTGGCCT TAAAGGTGAA AGGGGACGAC CTGGAGATCC TGGTATTGAA 
GGACCCATTG GACATCCTGG TCCTAAAGGA GAGCCTGGTC TCAAAGGAGA AAAGGGAGAA 
ATTGGAGCAC AAGGAAAAAA GGGTGTTGCA GGAATAGCTG GCCGTGATGG AAAAGATGGA 
CAGAAGGGAA AATTTGGCAG GATTGGTGCT CCTGGCTGCA AAGGTGATCC AGGTGACAAG 
GGTCCTGATG GTCATCCTGG AGATGTTGGA GAGCCTGGTC TTCCAGGCGC TTTGGGAGAT 
AAGGGTGATC GAGGTCGTCC AGGAAGATCA GGTCCTCCAG GCCCAGCTGG AGATCCTGGA 
CCAAAAGGAG AGAGAGGAAG TCCTGGATCG CCTGGAATTC CTGGCACAAA AGGAGGCGCT 
GGGACAGCTG GAGGACCTGG ACCCAAAGGA GATGCTGGGC GAAGAGGAGA TTTTGGACCT 
AAAGGAAGCA CTGGACCTAA TGGGGCAAAA GGAGAGAAGG GTGAACCAGG TGCTGAGGGA 
CCCAGAGGTC TTGCAGGTGA AGTTGGCGGC AAAGGATCCA AGGGTGATAA CGGACTTCCA 
GGACCAAGAG GCCCACCAGG AGCTCCTGGA GATGCAGGCA GAAAGGGTTC ATCAGGTGAC 
CCAGGTGATT CTGGCCCTAG AGGTGATCCT GGCCCTCCAG GACCAAAGGG TGATTCTGGC 
AGACCTGGAT TCAGCTATCC TGGACCAAGA GGACCAACGG GTGACAGGGG TGAAAAAGGT 
TCTCCTGGAC CCAGGGGAAG CAGAGGAGAC TGTGGGCCAA AGGGTGACCC AGGTCTAAAG 
GGAACTCCAG GAGAGCCGGG AGAACCTGGC CCAGCAGGAG AGCCTGGACA GAGGGGTCCT 
AGAGGAGATG GAGGAAGAGA TGGTGATCCT GGACCAGAGG GAGATCCTGG CCTTACTGAA 
TGTGATGTCA TGAACTACAT CAGAGAAACC TGTGGATGCT GTGATTGTGA AAAGAGATGC 
GGTGCCCTGG ATATTGTGTT TGTGATTGAC AGCTCTGAGA GTGTGGGGCT GACCAACTTC 
ACCCTGGAGA AGAATTTTGT GATCAACACC ATTAACAGAC TGGGCTCAAT CGCTAAAGAT 
CCCAGCTCTG AAACAGGAAC TCGAGTTGGT GTCGTCCAGT ACAGTCACAA CGGAACATTC 
CAGGCCATTC GACTCAACGA CTCCAAGATC GACTCCATGT CAGCCTTCAA AGATGCTGTG 
AAGAAGCTAG AGTGGATTGC TGGAGGAACG TGGACTCCTT CAGCCCTGAA GTTCGCCTAC 
GATAACCTGA TTCGTGACAG CCGTCGGTCA AAAGCCAATG TGACGGTGGT CGTAATCACA 
GATGGTCGGT ATGATCCCCG GGATGATGAC AAACTGCTCA ATTACCTCTG CACCGACACC 
AGCATTGATG TCAACGCTAT TGGTATTGGA GATATGTTTG ACCAGCCGGA GGAAAACGAA 
AGCTTGAAGT CTATCGCCTG TAGAAAAGAT GGAAGAGTCA TGGGAATGAG GCGTTTCGCT 
GATCTGGTCG CAGAAGATTT CATCGACAGG ATTGAGACTG TGCTTTGTCC GGATCCTGTG 
GTCATTTGCC CTGATCTTCC ATGTAAAAGT GAGCCAGCCG TGGCCAACTG CATCCAGCGG 
CCTGTTGATC TGGTCTTCAT GCTGGACGGC TCAGAGCGTA TGGGCCAGGA GAATTTCCGC 
TATGCACGTG AATTCGTGGA GAATGTGGCC AACCGTCTCA CACTAGCTCA GGGAGATGAT 
GATGAACGCA ATGCAAGGGT GGCTCTCCTG CAATATGGAG ATGAAAACCA ACATCAGTTG 
GCCTTCAAAC TAACAAATAA CTTCACCGTC ATCGCTGATG GCCTGGCCAA CATGAGGTAC 
CTAGATTCAA CCTCCAATGT TGGAAGTGGC ATCATCTACG CCATCAACAA TATCGTCACA 
AGCAGAGGCA CTCGATTGGC AAGACGAAAT GCAGAGTTGT CGTTTGTCTT CATTACCGAT 
GGGGTCACCA GTAACAAAAA CCTGGAAGAG GGGATTAGTG CCATGCGCAG AGCCGAGGGC 
GTTCCCACTG TCATTGCCAT GAGCAATGAT GTTGACAAGG AAATCCTTGC AAAGATTTCC 
CTGGGAGACC AGACGGCCGT TTTCAGGGGT GAAGATTTTG CCCGTCTGAA CAAAGCCAGC 
TTTTTTGAGC GATTCGTTCG GTGGATCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a2 ( XM_021479873.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335548.1 Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa148037
Clone ID Related Accession (Same CDS sequence)	XM_021479873.1
Accession Version	XM_021479873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGCT TGTCGATCCT CCTCCTGTTG TTTGTGGGTG TTCATGCACA GGGCCAGACT 
CCAGCCGGCT CTTCGTGTAG TCCTATAATG TGTTCTGTCC CTTCAGAGAG TACCGAATGC 
CCGATCGACT TGTACTTTGC TATTGACACA TCTGAGACCA TCGCTCTTCA GGAGAATCCG 
CCAGGCAGCT TGGTAGAGAG TATCAAAGAT TTCACCATCA GATTTGCTGA GAAGCTCCAA 
AATGTGAACT ACAGAGGATC CGTGAACATC ACCTGGGCCA TCGGAGGACT CCACTTCTCC 
CAGCTCCAGG AGTTCTTCAG CACCATCACC ACTAAAGAGA AGTTCATCAG TAATCTCCGG 
CCCATCAGAT ACCTAGGCAG GGGCACGCAC ATTGATTGCG CCATCACCAA CATGACCAAG 
CAGCTGGTCC GCTTTCCATC CAGACCCGAT GCCAAACGAT TCGCCGTGGT CATCACTGAT 
GGTCACGTGA CTGCGAACCC ATGCGGAGGG ATCAAAGTGG CGGCAGAACG GGCACGCGAT 
GAAAATATTA GGATCTTCGC TGTGGCATCG TCCAGGAATT TGGAGGAGAC GGGACTCAGG 
GAGATAGCAA ACTCACCCGC TGGAGTTTAC CGGGATAAAT ACATGGCGGT GAATCTCACT 
GGAACACGGC CGGTCATTGT GACGTCCACA ATCGATCGCA TTTATGACAC AATGCTTCAT 
ATGGCTTACC AAGAGTGCTA CTCTACCAAA TGCTTGGAGA CTCCAGGACC TCCAGGTCCC 
CCAGGACACA GAGGACAGAA AGGAGCTAAA GGAGATAATG GGGGCGCTGG CCTTAAAGGT 
GAAAGGGGAC GACCTGGAGA TCCTGGTATT GAAGGACCCA TTGGACATCC TGGTCCTAAA 
GGAGAGCCTG GTCTCAAAGG AGAAAAGGGA GAAATTGGAG CACAAGGAAA AAAGGGTGTT 
GCAGGAATAG CTGGCCGTGA TGGAAAAGAT GGACAGAAGG GAAAATTTGG CAGGATTGGT 
GCTCCTGGCT GCAAAGGTGA TCCAGGTGAC AAGGGTCCTG ATGGTCATCC TGGAGATGTT 
GGAGAGCCTG GTCTTCCAGG CGCTTTGGGA GATAAGGGTG ATCGAGGTCG TCCAGGAAGA 
TCAGGTCCTC CAGGCCCAGC TGGAGATCCT GGACCAAAAG GAGAGAGAGG AAGTCCTGGA 
TCGCCTGGAA TTCCTGGCAC AAAAGGAGGC GCTGGGACAG CTGGAGGACC TGGACCCAAA 
GGAGATGCTG GGCGAAGAGG AGATTTTGGA CCTAAAGGAA GCACTGGACC TAATGGGGCA 
AAAGGAGAGA AGGGTGAACC AGGTGCTGAG GGACCCAGAG GTCTTGCAGG TGAAGTTGGC 
GGCAAAGGAT CCAAGGGTGA TAACGGACTT CCAGGACCAA GAGGCCCACC AGGAGCTCCT 
GGAGATGCAG GCAGAAAGGG TTCATCAGGT GACCCAGGTG ATTCTGGCCC TAGAGGTGAT 
CCTGGCCCTC CAGGACCAAA GGGTGATTCT GGCAGACCTG GATTCAGCTA TCCTGGACCA 
AGAGGACCAA CGGGTGACAG GGGTGAAAAA GGTTCTCCTG GACCCAGGGG AAGCAGAGGA 
GACTGTGGGC CAAAGGGTGA CCCAGGTCTA AAGGGAACTC CAGGAGAGCC GGGAGAACCT 
GGCCCAGCAG GAGAGCCTGG ACAGAGGGGT CCTAGAGGAG ATGGAGGAAG AGATGGTGAT 
CCTGGACCAG AGGGAGATCC TGGCCTTACT GAATGTGATG TCATGAACTA CATCAGAGAA 
ACCTGTGGAT GCTGTGATTG TGAAAAGAGA TGCGGTGCCC TGGATATTGT GTTTGTGATT 
GACAGCTCTG AGAGTGTGGG GCTGACCAAC TTCACCCTGG AGAAGAATTT TGTGATCAAC 
ACCATTAACA GACTGGGCTC AATCGCTAAA GATCCCAGCT CTGAAACAGG AACTCGAGTT 
GGTGTCGTCC AGTACAGTCA CAACGGAACA TTCCAGGCCA TTCGACTCAA CGACTCCAAG 
ATCGACTCCA TGTCAGCCTT CAAAGATGCT GTGAAGAAGC TAGAGTGGAT TGCTGGAGGA 
ACGTGGACTC CTTCAGCCCT GAAGTTCGCC TACGATAACC TGATTCGTGA CAGCCGTCGG 
TCAAAAGCCA ATGTGACGGT GGTCGTAATC ACAGATGGTC GGTATGATCC CCGGGATGAT 
GACAAACTGC TCAATTACCT CTGCACCGAC ACCAGCATTG ATGTCAACGC TATTGGTATT 
GGAGATATGT TTGACCAGCC GGAGGAAAAC GAAAGCTTGA AGTCTATCGC CTGTAGAAAA 
GATGGAAGAG TCATGGGAAT GAGGCGTTTC GCTGATCTGG TCGCAGAAGA TTTCATCGAC 
AGGATTGAGA CTGTGCTTTG TCCGGATCCT GTGGTCATTT GCCCTGATCT TCCATGTAAA 
AGTGAGCCAG CCGTGGCCAA CTGCATCCAG CGGCCTGTTG ATCTGGTCTT CATGCTGGAC 
GGCTCAGAGC GTATGGGCCA GGAGAATTTC CGCTATGCAC GTGAATTCGT GGAGAATGTG 
GCCAACCGTC TCACACTAGC TCAGGGAGAT GATGATGAAC GCAATGCAAG GGTGGCTCTC 
CTGCAATATG GAGATGAAAA CCAACATCAG TTGGCCTTCA AACTAACAAA TAACTTCACC 
GTCATCGCTG ATGGCCTGGC CAACATGAGG TACCTAGATT CAACCTCCAA TGTTGGAAGT 
GGCATCATCT ACGCCATCAA CAATATCGTC ACAAGCAGAG GCACTCGATT GGCAAGACGA 
AATGCAGAGT TGTCGTTTGT CTTCATTACC GATGGGGTCA CCAGTAACAA AAACCTGGAA 
GAGGGGATTA GTGCCATGCG CAGAGCCGAG GGCGTTCCCA CTGTCATTGC CATGAGCAAT 
GATGTTGACA AGGAAATCCT TGCAAAGATT TCCCTGGGAG ACCAGACGGC CGTTTTCAGG 
GGTGAAGATT TTGCCCGTCT GAACAAAGCC AGCTTTTTTG AGCGATTCGT TCGGTGGATC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335548.1
CDS	172..3237
Translation	MMSLSILLLLFVGVHAQGQTPAGSSCSPIMCSVPSESTECPIDLYFAIDTSETIALQENPPGSLVESIKDFTIRFAEKLQNVNYRGSVNITWAIGGLHFSQLQEFFSTITTKEKFISNLRPIRYLGRGTHIDCAITNMTKQLVRFPSRPDAKRFAVVITDGHVTANPCGGIKVAAERARDENIRIFAVASSRNLEETGLREIANSPAGVYRDKYMAVNLTGTRPVIVTSTIDRIYDTMLHMAYQECYSTKCLETPGPPGPPGHRGQKGAKGDNGGAGLKGERGRPGDPGIEGPIGHPGPKGEPGLKGEKGEIGAQGKKGVAGIAGRDGKDGQKGKFGRIGAPGCKGDPGDKGPDGHPGDVGEPGLPGALGDKGDRGRPGRSGPPGPAGDPGPKGERGSPGSPGIPGTKGGAGTAGGPGPKGDAGRRGDFGPKGSTGPNGAKGEKGEPGAEGPRGLAGEVGGKGSKGDNGLPGPRGPPGAPGDAGRKGSSGDPGDSGPRGDPGPPGPKGDSGRPGFSYPGPRGPTGDRGEKGSPGPRGSRGDCGPKGDPGLKGTPGEPGEPGPAGEPGQRGPRGDGGRDGDPGPEGDPGLTECDVMNYIRETCGCCDCEKRCGALDIVFVIDSSESVGLTNFTLEKNFVINTINRLGSIAKDPSSETGTRVGVVQYSHNGTFQAIRLNDSKIDSMSAFKDAVKKLEWIAGGTWTPSALKFAYDNLIRDSRRSKANVTVVVITDGRYDPRDDDKLLNYLCTDTSIDVNAIGIGDMFDQPEENESLKSIACRKDGRVMGMRRFADLVAEDFIDRIETVLCPDPVVICPDLPCKSEPAVANCIQRPVDLVFMLDGSERMGQENFRYAREFVENVANRLTLAQGDDDERNARVALLQYGDENQHQLAFKLTNNFTVIADGLANMRYLDSTSNVGSGIIYAINNIVTSRGTRLARRNAELSFVFITDGVTSNKNLEEGISAMRRAEGVPTVIAMSNDVDKEILAKISLGDQTAVFRGEDFARLNKASFFERFVRWIC

Target ORF information:

RefSeq Version	XM_021479873.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479873.1

ORF Insert Sequence:

ATGATGAGCT TGTCGATCCT CCTCCTGTTG TTTGTGGGTG TTCATGCACA GGGCCAGACT 
CCAGCCGGCT CTTCGTGTAG TCCTATAATG TGTTCTGTCC CTTCAGAGAG TACCGAATGC 
CCGATCGACT TGTACTTTGC TATTGACACA TCTGAGACCA TCGCTCTTCA GGAGAATCCG 
CCAGGCAGCT TGGTAGAGAG TATCAAAGAT TTCACCATCA GATTTGCTGA GAAGCTCCAA 
AATGTGAACT ACAGAGGATC CGTGAACATC ACCTGGGCCA TCGGAGGACT CCACTTCTCC 
CAGCTCCAGG AGTTCTTCAG CACCATCACC ACTAAAGAGA AGTTCATCAG TAATCTCCGG 
CCCATCAGAT ACCTAGGCAG GGGCACGCAC ATTGATTGCG CCATCACCAA CATGACCAAG 
CAGCTGGTCC GCTTTCCATC CAGACCCGAT GCCAAACGAT TCGCCGTGGT CATCACTGAT 
GGTCACGTGA CTGCGAACCC ATGCGGAGGG ATCAAAGTGG CGGCAGAACG GGCACGCGAT 
GAAAATATTA GGATCTTCGC TGTGGCATCG TCCAGGAATT TGGAGGAGAC GGGACTCAGG 
GAGATAGCAA ACTCACCCGC TGGAGTTTAC CGGGATAAAT ACATGGCGGT GAATCTCACT 
GGAACACGGC CGGTCATTGT GACGTCCACA ATCGATCGCA TTTATGACAC AATGCTTCAT 
ATGGCTTACC AAGAGTGCTA CTCTACCAAA TGCTTGGAGA CTCCAGGACC TCCAGGTCCC 
CCAGGACACA GAGGACAGAA AGGAGCTAAA GGAGATAATG GGGGCGCTGG CCTTAAAGGT 
GAAAGGGGAC GACCTGGAGA TCCTGGTATT GAAGGACCCA TTGGACATCC TGGTCCTAAA 
GGAGAGCCTG GTCTCAAAGG AGAAAAGGGA GAAATTGGAG CACAAGGAAA AAAGGGTGTT 
GCAGGAATAG CTGGCCGTGA TGGAAAAGAT GGACAGAAGG GAAAATTTGG CAGGATTGGT 
GCTCCTGGCT GCAAAGGTGA TCCAGGTGAC AAGGGTCCTG ATGGTCATCC TGGAGATGTT 
GGAGAGCCTG GTCTTCCAGG CGCTTTGGGA GATAAGGGTG ATCGAGGTCG TCCAGGAAGA 
TCAGGTCCTC CAGGCCCAGC TGGAGATCCT GGACCAAAAG GAGAGAGAGG AAGTCCTGGA 
TCGCCTGGAA TTCCTGGCAC AAAAGGAGGC GCTGGGACAG CTGGAGGACC TGGACCCAAA 
GGAGATGCTG GGCGAAGAGG AGATTTTGGA CCTAAAGGAA GCACTGGACC TAATGGGGCA 
AAAGGAGAGA AGGGTGAACC AGGTGCTGAG GGACCCAGAG GTCTTGCAGG TGAAGTTGGC 
GGCAAAGGAT CCAAGGGTGA TAACGGACTT CCAGGACCAA GAGGCCCACC AGGAGCTCCT 
GGAGATGCAG GCAGAAAGGG TTCATCAGGT GACCCAGGTG ATTCTGGCCC TAGAGGTGAT 
CCTGGCCCTC CAGGACCAAA GGGTGATTCT GGCAGACCTG GATTCAGCTA TCCTGGACCA 
AGAGGACCAA CGGGTGACAG GGGTGAAAAA GGTTCTCCTG GACCCAGGGG AAGCAGAGGA 
GACTGTGGGC CAAAGGGTGA CCCAGGTCTA AAGGGAACTC CAGGAGAGCC GGGAGAACCT 
GGCCCAGCAG GAGAGCCTGG ACAGAGGGGT CCTAGAGGAG ATGGAGGAAG AGATGGTGAT 
CCTGGACCAG AGGGAGATCC TGGCCTTACT GAATGTGATG TCATGAACTA CATCAGAGAA 
ACCTGTGGAT GCTGTGATTG TGAAAAGAGA TGCGGTGCCC TGGATATTGT GTTTGTGATT 
GACAGCTCTG AGAGTGTGGG GCTGACCAAC TTCACCCTGG AGAAGAATTT TGTGATCAAC 
ACCATTAACA GACTGGGCTC AATCGCTAAA GATCCCAGCT CTGAAACAGG AACTCGAGTT 
GGTGTCGTCC AGTACAGTCA CAACGGAACA TTCCAGGCCA TTCGACTCAA CGACTCCAAG 
ATCGACTCCA TGTCAGCCTT CAAAGATGCT GTGAAGAAGC TAGAGTGGAT TGCTGGAGGA 
ACGTGGACTC CTTCAGCCCT GAAGTTCGCC TACGATAACC TGATTCGTGA CAGCCGTCGG 
TCAAAAGCCA ATGTGACGGT GGTCGTAATC ACAGATGGTC GGTATGATCC CCGGGATGAT 
GACAAACTGC TCAATTACCT CTGCACCGAC ACCAGCATTG ATGTCAACGC TATTGGTATT 
GGAGATATGT TTGACCAGCC GGAGGAAAAC GAAAGCTTGA AGTCTATCGC CTGTAGAAAA 
GATGGAAGAG TCATGGGAAT GAGGCGTTTC GCTGATCTGG TCGCAGAAGA TTTCATCGAC 
AGGATTGAGA CTGTGCTTTG TCCGGATCCT GTGGTCATTT GCCCTGATCT TCCATGTAAA 
AGTGAGCCAG CCGTGGCCAA CTGCATCCAG CGGCCTGTTG ATCTGGTCTT CATGCTGGAC 
GGCTCAGAGC GTATGGGCCA GGAGAATTTC CGCTATGCAC GTGAATTCGT GGAGAATGTG 
GCCAACCGTC TCACACTAGC TCAGGGAGAT GATGATGAAC GCAATGCAAG GGTGGCTCTC 
CTGCAATATG GAGATGAAAA CCAACATCAG TTGGCCTTCA AACTAACAAA TAACTTCACC 
GTCATCGCTG ATGGCCTGGC CAACATGAGG TACCTAGATT CAACCTCCAA TGTTGGAAGT 
GGCATCATCT ACGCCATCAA CAATATCGTC ACAAGCAGAG GCACTCGATT GGCAAGACGA 
AATGCAGAGT TGTCGTTTGT CTTCATTACC GATGGGGTCA CCAGTAACAA AAACCTGGAA 
GAGGGGATTA GTGCCATGCG CAGAGCCGAG GGCGTTCCCA CTGTCATTGC CATGAGCAAT 
GATGTTGACA AGGAAATCCT TGCAAAGATT TCCCTGGGAG ACCAGACGGC CGTTTTCAGG 
GGTGAAGATT TTGCCCGTCT GAACAAAGCC AGCTTTTTTG AGCGATTCGT TCGGTGGATC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a2 ( XM_691072.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_696164.2 Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa17233
Clone ID Related Accession (Same CDS sequence)	XM_691072.8 , XM_691072.7
Accession Version	XM_691072.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(VI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_691072.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGCT TGTCGATCCT CCTCCTGTTG TTTGTGGGTG TTCATGCACA GGGCCAGACT 
CCAGCCGGCT CTTCGTGTAG TCCTATAAAG AGTACCGAAT GCCCGATCGA CTTGTACTTT 
GCTATTGACA CATCTGAGAC CATCGCTCTT CAGGAGAATC CGCCAGGCAG CTTGGTAGAG 
AGTATCAAAG ATTTCACCAT CAGATTTGCT GAGAAGCTCC AAAATGTGAA CTACAGAGGA 
TCCGTGAACA TCACCTGGGC CATCGGAGGA CTCCACTTCT CCCAGCTCCA GGAGTTCTTC 
AGCACCATCA CCACTAAAGA GAAGTTCATC AGTAATCTCC GGCCCATCAG ATACCTAGGC 
AGGGGCACGC ACATTGATTG CGCCATCACC AACATGACCA AGCAGCTGGT CCGCTTTCCA 
TCCAGACCCG ATGCCAAACG ATTCGCCGTG GTCATCACTG ATGGTCACGT GACTGCGAAC 
CCATGCGGAG GGATCAAAGT GGCGGCAGAA CGGGCACGCG ATGAAAATAT TAGGATCTTC 
GCTGTGGCAT CGTCCAGGAA TTTGGAGGAG ACGGGACTCA GGGAGATAGC AAACTCACCC 
GCTGGAGTTT ACCGGGATAA ATACATGGCG GTGAATCTCA CTGGAACACG GCCGGTCATT 
GTGACGTCCA CAATCGATCG CATTTATGAC ACAATGCTTC ATATGGCTTA CCAAGAGTGC 
TACTCTACCA AATGCTTGGA GACTCCAGGA CCTCCAGGTC CCCCAGGACA CAGAGGACAG 
AAAGGAGCTA AAGGAGATAA TGGGGGCGCT GGCCTTAAAG GTGAAAGGGG ACGACCTGGA 
GATCCTGGTA TTGAAGGACC CATTGGACAT CCTGGTCCTA AAGGAGAGCC TGGTCTCAAA 
GGAGAAAAGG GAGAAATTGG AGCACAAGGA AAAAAGGGTG TTGCAGGAAT AGCTGGCCGT 
GATGGAAAAG ATGGACAGAA GGGAAAATTT GGCAGGATTG GTGCTCCTGG CTGCAAAGGT 
GATCCAGGTG ACAAGGGTCC TGATGGTCAT CCTGGAGATG TTGGAGAGCC TGGTCTTCCA 
GGCGCTTTGG GAGATAAGGG TGATCGAGGT CGTCCAGGAA GATCAGGTCC TCCAGGCCCA 
GCTGGAGATC CTGGACCAAA AGGAGAGAGA GGAAGTCCTG GATCGCCTGG AATTCCTGGC 
ACAAAAGGAG GCGCTGGGAC AGCTGGAGGA CCTGGACCCA AAGGAGATGC TGGGCGAAGA 
GGAGATTTTG GACCTAAAGG AAGCACTGGA CCTAATGGGG CAAAAGGAGA GAAGGGTGAA 
CCAGGTGCTG AGGGACCCAG AGGTCTTGCA GGTGAAGTTG GCGGCAAAGG ATCCAAGGGT 
GATAACGGAC TTCCAGGACC AAGAGGCCCA CCAGGAGCTC CTGGAGATGC AGGCAGAAAG 
GGTTCATCAG GTGACCCAGG TGATTCTGGC CCTAGAGGTG ATCCTGGCCC TCCAGGACCA 
AAGGGTGATT CTGGCAGACC TGGATTCAGC TATCCTGGAC CAAGAGGACC AACGGGTGAC 
AGGGGTGAAA AAGGTTCTCC TGGACCCAGG GGAAGCAGAG GAGACTGTGG GCCAAAGGGT 
GACCCAGGTC TAAAGGGAAC TCCAGGAGAG CCGGGAGAAC CTGGCCCAGC AGGAGAGCCT 
GGACAGAGGG GTCCTAGAGG AGATGGAGGA AGAGATGGTG ATCCTGGACC AGAGGGAGAT 
CCTGGCCTTA CTGAATGTGA TGTCATGAAC TACATCAGAG AAACCTGTGG ATGCTGTGAT 
TGTGAAAAGA GATGCGGTGC CCTGGATATT GTGTTTGTGA TTGACAGCTC TGAGAGTGTG 
GGGCTGACCA ACTTCACCCT GGAGAAGAAT TTTGTGATCA ACACCATTAA CAGACTGGGC 
TCAATCGCTA AAGATCCCAG CTCTGAAACA GGAACTCGAG TTGGTGTCGT CCAGTACAGT 
CACAACGGAA CATTCCAGGC CATTCGACTC AACGACTCCA AGATCGACTC CATGTCAGCC 
TTCAAAGATG CTGTGAAGAA GCTAGAGTGG ATTGCTGGAG GAACGTGGAC TCCTTCAGCC 
CTGAAGTTCG CCTACGATAA CCTGATTCGT GACAGCCGTC GGTCAAAAGC CAATGTGACG 
GTGGTCGTAA TCACAGATGG TCGGTATGAT CCCCGGGATG ATGACAAACT GCTCAATTAC 
CTCTGCACCG ACACCAGCAT TGATGTCAAC GCTATTGGTA TTGGAGATAT GTTTGACCAG 
CCGGAGGAAA ACGAAAGCTT GAAGTCTATC GCCTGTAGAA AAGATGGAAG AGTCATGGGA 
ATGAGGCGTT TCGCTGATCT GGTCGCAGAA GATTTCATCG ACAGGATTGA GACTGTGCTT 
TGTCCGGATC CTGTGGTCAT TTGCCCTGAT CTTCCATGTA AAAGTGAGCC AGCCGTGGCC 
AACTGCATCC AGCGGCCTGT TGATCTGGTC TTCATGCTGG ACGGCTCAGA GCGTATGGGC 
CAGGAGAATT TCCGCTATGC ACGTGAATTC GTGGAGAATG TGGCCAACCG TCTCACACTA 
GCTCAGGGAG ATGATGATGA ACGCAATGCA AGGGTGGCTC TCCTGCAATA TGGAGATGAA 
AACCAACATC AGTTGGCCTT CAAACTAACA AATAACTTCA CCGTCATCGC TGATGGCCTG 
GCCAACATGA GGTACCTAGA TTCAACCTCC AATGTTGGAA GTGGCATCAT CTACGCCATC 
AACAATATCG TCACAAGCAG AGGCACTCGA TTGGCAAGAC GAAATGCAGA GTTGTCGTTT 
GTCTTCATTA CCGATGGGGT CACCAGTAAC AAAAACCTGG AAGAGGGGAT TAGTGCCATG 
CGCAGAGCCG AGGGCGTTCC CACTGTCATT GCCATGAGCA ATGATGTTGA CAAGGAAATC 
CTTGCAAAGA TTTCCCTGGG AGACCAGACG GCCGTTTTCA GGGGTGAAGA TTTTGCCCGT 
CTGAACAAAG CCAGCTTTTT TGAGCGATTC GTTCGGTGGA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_696164.2
CDS	186..3233
Translation	MMSLSILLLLFVGVHAQGQTPAGSSCSPIKSTECPIDLYFAIDTSETIALQENPPGSLVESIKDFTIRFAEKLQNVNYRGSVNITWAIGGLHFSQLQEFFSTITTKEKFISNLRPIRYLGRGTHIDCAITNMTKQLVRFPSRPDAKRFAVVITDGHVTANPCGGIKVAAERARDENIRIFAVASSRNLEETGLREIANSPAGVYRDKYMAVNLTGTRPVIVTSTIDRIYDTMLHMAYQECYSTKCLETPGPPGPPGHRGQKGAKGDNGGAGLKGERGRPGDPGIEGPIGHPGPKGEPGLKGEKGEIGAQGKKGVAGIAGRDGKDGQKGKFGRIGAPGCKGDPGDKGPDGHPGDVGEPGLPGALGDKGDRGRPGRSGPPGPAGDPGPKGERGSPGSPGIPGTKGGAGTAGGPGPKGDAGRRGDFGPKGSTGPNGAKGEKGEPGAEGPRGLAGEVGGKGSKGDNGLPGPRGPPGAPGDAGRKGSSGDPGDSGPRGDPGPPGPKGDSGRPGFSYPGPRGPTGDRGEKGSPGPRGSRGDCGPKGDPGLKGTPGEPGEPGPAGEPGQRGPRGDGGRDGDPGPEGDPGLTECDVMNYIRETCGCCDCEKRCGALDIVFVIDSSESVGLTNFTLEKNFVINTINRLGSIAKDPSSETGTRVGVVQYSHNGTFQAIRLNDSKIDSMSAFKDAVKKLEWIAGGTWTPSALKFAYDNLIRDSRRSKANVTVVVITDGRYDPRDDDKLLNYLCTDTSIDVNAIGIGDMFDQPEENESLKSIACRKDGRVMGMRRFADLVAEDFIDRIETVLCPDPVVICPDLPCKSEPAVANCIQRPVDLVFMLDGSERMGQENFRYAREFVENVANRLTLAQGDDDERNARVALLQYGDENQHQLAFKLTNNFTVIADGLANMRYLDSTSNVGSGIIYAINNIVTSRGTRLARRNAELSFVFITDGVTSNKNLEEGISAMRRAEGVPTVIAMSNDVDKEILAKISLGDQTAVFRGEDFARLNKASFFERFVRWIC

Target ORF information:

RefSeq Version	XM_691072.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_691072.8

ORF Insert Sequence:

ATGATGAGCT TGTCGATCCT CCTCCTGTTG TTTGTGGGTG TTCATGCACA GGGCCAGACT 
CCAGCCGGCT CTTCGTGTAG TCCTATAAAG AGTACCGAAT GCCCGATCGA CTTGTACTTT 
GCTATTGACA CATCTGAGAC CATCGCTCTT CAGGAGAATC CGCCAGGCAG CTTGGTAGAG 
AGTATCAAAG ATTTCACCAT CAGATTTGCT GAGAAGCTCC AAAATGTGAA CTACAGAGGA 
TCCGTGAACA TCACCTGGGC CATCGGAGGA CTCCACTTCT CCCAGCTCCA GGAGTTCTTC 
AGCACCATCA CCACTAAAGA GAAGTTCATC AGTAATCTCC GGCCCATCAG ATACCTAGGC 
AGGGGCACGC ACATTGATTG CGCCATCACC AACATGACCA AGCAGCTGGT CCGCTTTCCA 
TCCAGACCCG ATGCCAAACG ATTCGCCGTG GTCATCACTG ATGGTCACGT GACTGCGAAC 
CCATGCGGAG GGATCAAAGT GGCGGCAGAA CGGGCACGCG ATGAAAATAT TAGGATCTTC 
GCTGTGGCAT CGTCCAGGAA TTTGGAGGAG ACGGGACTCA GGGAGATAGC AAACTCACCC 
GCTGGAGTTT ACCGGGATAA ATACATGGCG GTGAATCTCA CTGGAACACG GCCGGTCATT 
GTGACGTCCA CAATCGATCG CATTTATGAC ACAATGCTTC ATATGGCTTA CCAAGAGTGC 
TACTCTACCA AATGCTTGGA GACTCCAGGA CCTCCAGGTC CCCCAGGACA CAGAGGACAG 
AAAGGAGCTA AAGGAGATAA TGGGGGCGCT GGCCTTAAAG GTGAAAGGGG ACGACCTGGA 
GATCCTGGTA TTGAAGGACC CATTGGACAT CCTGGTCCTA AAGGAGAGCC TGGTCTCAAA 
GGAGAAAAGG GAGAAATTGG AGCACAAGGA AAAAAGGGTG TTGCAGGAAT AGCTGGCCGT 
GATGGAAAAG ATGGACAGAA GGGAAAATTT GGCAGGATTG GTGCTCCTGG CTGCAAAGGT 
GATCCAGGTG ACAAGGGTCC TGATGGTCAT CCTGGAGATG TTGGAGAGCC TGGTCTTCCA 
GGCGCTTTGG GAGATAAGGG TGATCGAGGT CGTCCAGGAA GATCAGGTCC TCCAGGCCCA 
GCTGGAGATC CTGGACCAAA AGGAGAGAGA GGAAGTCCTG GATCGCCTGG AATTCCTGGC 
ACAAAAGGAG GCGCTGGGAC AGCTGGAGGA CCTGGACCCA AAGGAGATGC TGGGCGAAGA 
GGAGATTTTG GACCTAAAGG AAGCACTGGA CCTAATGGGG CAAAAGGAGA GAAGGGTGAA 
CCAGGTGCTG AGGGACCCAG AGGTCTTGCA GGTGAAGTTG GCGGCAAAGG ATCCAAGGGT 
GATAACGGAC TTCCAGGACC AAGAGGCCCA CCAGGAGCTC CTGGAGATGC AGGCAGAAAG 
GGTTCATCAG GTGACCCAGG TGATTCTGGC CCTAGAGGTG ATCCTGGCCC TCCAGGACCA 
AAGGGTGATT CTGGCAGACC TGGATTCAGC TATCCTGGAC CAAGAGGACC AACGGGTGAC 
AGGGGTGAAA AAGGTTCTCC TGGACCCAGG GGAAGCAGAG GAGACTGTGG GCCAAAGGGT 
GACCCAGGTC TAAAGGGAAC TCCAGGAGAG CCGGGAGAAC CTGGCCCAGC AGGAGAGCCT 
GGACAGAGGG GTCCTAGAGG AGATGGAGGA AGAGATGGTG ATCCTGGACC AGAGGGAGAT 
CCTGGCCTTA CTGAATGTGA TGTCATGAAC TACATCAGAG AAACCTGTGG ATGCTGTGAT 
TGTGAAAAGA GATGCGGTGC CCTGGATATT GTGTTTGTGA TTGACAGCTC TGAGAGTGTG 
GGGCTGACCA ACTTCACCCT GGAGAAGAAT TTTGTGATCA ACACCATTAA CAGACTGGGC 
TCAATCGCTA AAGATCCCAG CTCTGAAACA GGAACTCGAG TTGGTGTCGT CCAGTACAGT 
CACAACGGAA CATTCCAGGC CATTCGACTC AACGACTCCA AGATCGACTC CATGTCAGCC 
TTCAAAGATG CTGTGAAGAA GCTAGAGTGG ATTGCTGGAG GAACGTGGAC TCCTTCAGCC 
CTGAAGTTCG CCTACGATAA CCTGATTCGT GACAGCCGTC GGTCAAAAGC CAATGTGACG 
GTGGTCGTAA TCACAGATGG TCGGTATGAT CCCCGGGATG ATGACAAACT GCTCAATTAC 
CTCTGCACCG ACACCAGCAT TGATGTCAAC GCTATTGGTA TTGGAGATAT GTTTGACCAG 
CCGGAGGAAA ACGAAAGCTT GAAGTCTATC GCCTGTAGAA AAGATGGAAG AGTCATGGGA 
ATGAGGCGTT TCGCTGATCT GGTCGCAGAA GATTTCATCG ACAGGATTGA GACTGTGCTT 
TGTCCGGATC CTGTGGTCAT TTGCCCTGAT CTTCCATGTA AAAGTGAGCC AGCCGTGGCC 
AACTGCATCC AGCGGCCTGT TGATCTGGTC TTCATGCTGG ACGGCTCAGA GCGTATGGGC 
CAGGAGAATT TCCGCTATGC ACGTGAATTC GTGGAGAATG TGGCCAACCG TCTCACACTA 
GCTCAGGGAG ATGATGATGA ACGCAATGCA AGGGTGGCTC TCCTGCAATA TGGAGATGAA 
AACCAACATC AGTTGGCCTT CAAACTAACA AATAACTTCA CCGTCATCGC TGATGGCCTG 
GCCAACATGA GGTACCTAGA TTCAACCTCC AATGTTGGAA GTGGCATCAT CTACGCCATC 
AACAATATCG TCACAAGCAG AGGCACTCGA TTGGCAAGAC GAAATGCAGA GTTGTCGTTT 
GTCTTCATTA CCGATGGGGT CACCAGTAAC AAAAACCTGG AAGAGGGGAT TAGTGCCATG 
CGCAGAGCCG AGGGCGTTCC CACTGTCATT GCCATGAGCA ATGATGTTGA CAAGGAAATC 
CTTGCAAAGA TTTCCCTGGG AGACCAGACG GCCGTTTTCA GGGGTGAAGA TTTTGCCCGT 
CTGAACAAAG CCAGCTTTTT TGAGCGATTC GTTCGGTGGA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a2 ( XM_691072.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_696164.2 Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17233
Clone ID Related Accession (Same CDS sequence)	XM_691072.8 , XM_691072.7
Accession Version	XM_691072.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(VI) chain isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_691072.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGCT TGTCGATCCT CCTCCTGTTG TTTGTGGGTG TTCATGCACA GGGCCAGACT 
CCAGCCGGCT CTTCGTGTAG TCCTATAAAG AGTACCGAAT GCCCGATCGA CTTGTACTTT 
GCTATTGACA CATCTGAGAC CATCGCTCTT CAGGAGAATC CGCCAGGCAG CTTGGTAGAG 
AGTATCAAAG ATTTCACCAT CAGATTTGCT GAGAAGCTCC AAAATGTGAA CTACAGAGGA 
TCCGTGAACA TCACCTGGGC CATCGGAGGA CTCCACTTCT CCCAGCTCCA GGAGTTCTTC 
AGCACCATCA CCACTAAAGA GAAGTTCATC AGTAATCTCC GGCCCATCAG ATACCTAGGC 
AGGGGCACGC ACATTGATTG CGCCATCACC AACATGACCA AGCAGCTGGT CCGCTTTCCA 
TCCAGACCCG ATGCCAAACG ATTCGCCGTG GTCATCACTG ATGGTCACGT GACTGCGAAC 
CCATGCGGAG GGATCAAAGT GGCGGCAGAA CGGGCACGCG ATGAAAATAT TAGGATCTTC 
GCTGTGGCAT CGTCCAGGAA TTTGGAGGAG ACGGGACTCA GGGAGATAGC AAACTCACCC 
GCTGGAGTTT ACCGGGATAA ATACATGGCG GTGAATCTCA CTGGAACACG GCCGGTCATT 
GTGACGTCCA CAATCGATCG CATTTATGAC ACAATGCTTC ATATGGCTTA CCAAGAGTGC 
TACTCTACCA AATGCTTGGA GACTCCAGGA CCTCCAGGTC CCCCAGGACA CAGAGGACAG 
AAAGGAGCTA AAGGAGATAA TGGGGGCGCT GGCCTTAAAG GTGAAAGGGG ACGACCTGGA 
GATCCTGGTA TTGAAGGACC CATTGGACAT CCTGGTCCTA AAGGAGAGCC TGGTCTCAAA 
GGAGAAAAGG GAGAAATTGG AGCACAAGGA AAAAAGGGTG TTGCAGGAAT AGCTGGCCGT 
GATGGAAAAG ATGGACAGAA GGGAAAATTT GGCAGGATTG GTGCTCCTGG CTGCAAAGGT 
GATCCAGGTG ACAAGGGTCC TGATGGTCAT CCTGGAGATG TTGGAGAGCC TGGTCTTCCA 
GGCGCTTTGG GAGATAAGGG TGATCGAGGT CGTCCAGGAA GATCAGGTCC TCCAGGCCCA 
GCTGGAGATC CTGGACCAAA AGGAGAGAGA GGAAGTCCTG GATCGCCTGG AATTCCTGGC 
ACAAAAGGAG GCGCTGGGAC AGCTGGAGGA CCTGGACCCA AAGGAGATGC TGGGCGAAGA 
GGAGATTTTG GACCTAAAGG AAGCACTGGA CCTAATGGGG CAAAAGGAGA GAAGGGTGAA 
CCAGGTGCTG AGGGACCCAG AGGTCTTGCA GGTGAAGTTG GCGGCAAAGG ATCCAAGGGT 
GATAACGGAC TTCCAGGACC AAGAGGCCCA CCAGGAGCTC CTGGAGATGC AGGCAGAAAG 
GGTTCATCAG GTGACCCAGG TGATTCTGGC CCTAGAGGTG ATCCTGGCCC TCCAGGACCA 
AAGGGTGATT CTGGCAGACC TGGATTCAGC TATCCTGGAC CAAGAGGACC AACGGGTGAC 
AGGGGTGAAA AAGGTTCTCC TGGACCCAGG GGAAGCAGAG GAGACTGTGG GCCAAAGGGT 
GACCCAGGTC TAAAGGGAAC TCCAGGAGAG CCGGGAGAAC CTGGCCCAGC AGGAGAGCCT 
GGACAGAGGG GTCCTAGAGG AGATGGAGGA AGAGATGGTG ATCCTGGACC AGAGGGAGAT 
CCTGGCCTTA CTGAATGTGA TGTCATGAAC TACATCAGAG AAACCTGTGG ATGCTGTGAT 
TGTGAAAAGA GATGCGGTGC CCTGGATATT GTGTTTGTGA TTGACAGCTC TGAGAGTGTG 
GGGCTGACCA ACTTCACCCT GGAGAAGAAT TTTGTGATCA ACACCATTAA CAGACTGGGC 
TCAATCGCTA AAGATCCCAG CTCTGAAACA GGAACTCGAG TTGGTGTCGT CCAGTACAGT 
CACAACGGAA CATTCCAGGC CATTCGACTC AACGACTCCA AGATCGACTC CATGTCAGCC 
TTCAAAGATG CTGTGAAGAA GCTAGAGTGG ATTGCTGGAG GAACGTGGAC TCCTTCAGCC 
CTGAAGTTCG CCTACGATAA CCTGATTCGT GACAGCCGTC GGTCAAAAGC CAATGTGACG 
GTGGTCGTAA TCACAGATGG TCGGTATGAT CCCCGGGATG ATGACAAACT GCTCAATTAC 
CTCTGCACCG ACACCAGCAT TGATGTCAAC GCTATTGGTA TTGGAGATAT GTTTGACCAG 
CCGGAGGAAA ACGAAAGCTT GAAGTCTATC GCCTGTAGAA AAGATGGAAG AGTCATGGGA 
ATGAGGCGTT TCGCTGATCT GGTCGCAGAA GATTTCATCG ACAGGATTGA GACTGTGCTT 
TGTCCGGATC CTGTGGTCAT TTGCCCTGAT CTTCCATGTA AAAGTGAGCC AGCCGTGGCC 
AACTGCATCC AGCGGCCTGT TGATCTGGTC TTCATGCTGG ACGGCTCAGA GCGTATGGGC 
CAGGAGAATT TCCGCTATGC ACGTGAATTC GTGGAGAATG TGGCCAACCG TCTCACACTA 
GCTCAGGGAG ATGATGATGA ACGCAATGCA AGGGTGGCTC TCCTGCAATA TGGAGATGAA 
AACCAACATC AGTTGGCCTT CAAACTAACA AATAACTTCA CCGTCATCGC TGATGGCCTG 
GCCAACATGA GGTACCTAGA TTCAACCTCC AATGTTGGAA GTGGCATCAT CTACGCCATC 
AACAATATCG TCACAAGCAG AGGCACTCGA TTGGCAAGAC GAAATGCAGA GTTGTCGTTT 
GTCTTCATTA CCGATGGGGT CACCAGTAAC AAAAACCTGG AAGAGGGGAT TAGTGCCATG 
CGCAGAGCCG AGGGCGTTCC CACTGTCATT GCCATGAGCA ATGATGTTGA CAAGGAAATC 
CTTGCAAAGA TTTCCCTGGG AGACCAGACG GCCGTTTTCA GGGGTGAAGA TTTTGCCCGT 
CTGAACAAAG CCAGCTTTTT TGAGCGATTC GTTCGGTGGA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_696164.2
CDS	186..3233
Translation	MMSLSILLLLFVGVHAQGQTPAGSSCSPIKSTECPIDLYFAIDTSETIALQENPPGSLVESIKDFTIRFAEKLQNVNYRGSVNITWAIGGLHFSQLQEFFSTITTKEKFISNLRPIRYLGRGTHIDCAITNMTKQLVRFPSRPDAKRFAVVITDGHVTANPCGGIKVAAERARDENIRIFAVASSRNLEETGLREIANSPAGVYRDKYMAVNLTGTRPVIVTSTIDRIYDTMLHMAYQECYSTKCLETPGPPGPPGHRGQKGAKGDNGGAGLKGERGRPGDPGIEGPIGHPGPKGEPGLKGEKGEIGAQGKKGVAGIAGRDGKDGQKGKFGRIGAPGCKGDPGDKGPDGHPGDVGEPGLPGALGDKGDRGRPGRSGPPGPAGDPGPKGERGSPGSPGIPGTKGGAGTAGGPGPKGDAGRRGDFGPKGSTGPNGAKGEKGEPGAEGPRGLAGEVGGKGSKGDNGLPGPRGPPGAPGDAGRKGSSGDPGDSGPRGDPGPPGPKGDSGRPGFSYPGPRGPTGDRGEKGSPGPRGSRGDCGPKGDPGLKGTPGEPGEPGPAGEPGQRGPRGDGGRDGDPGPEGDPGLTECDVMNYIRETCGCCDCEKRCGALDIVFVIDSSESVGLTNFTLEKNFVINTINRLGSIAKDPSSETGTRVGVVQYSHNGTFQAIRLNDSKIDSMSAFKDAVKKLEWIAGGTWTPSALKFAYDNLIRDSRRSKANVTVVVITDGRYDPRDDDKLLNYLCTDTSIDVNAIGIGDMFDQPEENESLKSIACRKDGRVMGMRRFADLVAEDFIDRIETVLCPDPVVICPDLPCKSEPAVANCIQRPVDLVFMLDGSERMGQENFRYAREFVENVANRLTLAQGDDDERNARVALLQYGDENQHQLAFKLTNNFTVIADGLANMRYLDSTSNVGSGIIYAINNIVTSRGTRLARRNAELSFVFITDGVTSNKNLEEGISAMRRAEGVPTVIAMSNDVDKEILAKISLGDQTAVFRGEDFARLNKASFFERFVRWIC

Target ORF information:

RefSeq Version	XM_691072.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_691072.7

ORF Insert Sequence:

ATGATGAGCT TGTCGATCCT CCTCCTGTTG TTTGTGGGTG TTCATGCACA GGGCCAGACT 
CCAGCCGGCT CTTCGTGTAG TCCTATAAAG AGTACCGAAT GCCCGATCGA CTTGTACTTT 
GCTATTGACA CATCTGAGAC CATCGCTCTT CAGGAGAATC CGCCAGGCAG CTTGGTAGAG 
AGTATCAAAG ATTTCACCAT CAGATTTGCT GAGAAGCTCC AAAATGTGAA CTACAGAGGA 
TCCGTGAACA TCACCTGGGC CATCGGAGGA CTCCACTTCT CCCAGCTCCA GGAGTTCTTC 
AGCACCATCA CCACTAAAGA GAAGTTCATC AGTAATCTCC GGCCCATCAG ATACCTAGGC 
AGGGGCACGC ACATTGATTG CGCCATCACC AACATGACCA AGCAGCTGGT CCGCTTTCCA 
TCCAGACCCG ATGCCAAACG ATTCGCCGTG GTCATCACTG ATGGTCACGT GACTGCGAAC 
CCATGCGGAG GGATCAAAGT GGCGGCAGAA CGGGCACGCG ATGAAAATAT TAGGATCTTC 
GCTGTGGCAT CGTCCAGGAA TTTGGAGGAG ACGGGACTCA GGGAGATAGC AAACTCACCC 
GCTGGAGTTT ACCGGGATAA ATACATGGCG GTGAATCTCA CTGGAACACG GCCGGTCATT 
GTGACGTCCA CAATCGATCG CATTTATGAC ACAATGCTTC ATATGGCTTA CCAAGAGTGC 
TACTCTACCA AATGCTTGGA GACTCCAGGA CCTCCAGGTC CCCCAGGACA CAGAGGACAG 
AAAGGAGCTA AAGGAGATAA TGGGGGCGCT GGCCTTAAAG GTGAAAGGGG ACGACCTGGA 
GATCCTGGTA TTGAAGGACC CATTGGACAT CCTGGTCCTA AAGGAGAGCC TGGTCTCAAA 
GGAGAAAAGG GAGAAATTGG AGCACAAGGA AAAAAGGGTG TTGCAGGAAT AGCTGGCCGT 
GATGGAAAAG ATGGACAGAA GGGAAAATTT GGCAGGATTG GTGCTCCTGG CTGCAAAGGT 
GATCCAGGTG ACAAGGGTCC TGATGGTCAT CCTGGAGATG TTGGAGAGCC TGGTCTTCCA 
GGCGCTTTGG GAGATAAGGG TGATCGAGGT CGTCCAGGAA GATCAGGTCC TCCAGGCCCA 
GCTGGAGATC CTGGACCAAA AGGAGAGAGA GGAAGTCCTG GATCGCCTGG AATTCCTGGC 
ACAAAAGGAG GCGCTGGGAC AGCTGGAGGA CCTGGACCCA AAGGAGATGC TGGGCGAAGA 
GGAGATTTTG GACCTAAAGG AAGCACTGGA CCTAATGGGG CAAAAGGAGA GAAGGGTGAA 
CCAGGTGCTG AGGGACCCAG AGGTCTTGCA GGTGAAGTTG GCGGCAAAGG ATCCAAGGGT 
GATAACGGAC TTCCAGGACC AAGAGGCCCA CCAGGAGCTC CTGGAGATGC AGGCAGAAAG 
GGTTCATCAG GTGACCCAGG TGATTCTGGC CCTAGAGGTG ATCCTGGCCC TCCAGGACCA 
AAGGGTGATT CTGGCAGACC TGGATTCAGC TATCCTGGAC CAAGAGGACC AACGGGTGAC 
AGGGGTGAAA AAGGTTCTCC TGGACCCAGG GGAAGCAGAG GAGACTGTGG GCCAAAGGGT 
GACCCAGGTC TAAAGGGAAC TCCAGGAGAG CCGGGAGAAC CTGGCCCAGC AGGAGAGCCT 
GGACAGAGGG GTCCTAGAGG AGATGGAGGA AGAGATGGTG ATCCTGGACC AGAGGGAGAT 
CCTGGCCTTA CTGAATGTGA TGTCATGAAC TACATCAGAG AAACCTGTGG ATGCTGTGAT 
TGTGAAAAGA GATGCGGTGC CCTGGATATT GTGTTTGTGA TTGACAGCTC TGAGAGTGTG 
GGGCTGACCA ACTTCACCCT GGAGAAGAAT TTTGTGATCA ACACCATTAA CAGACTGGGC 
TCAATCGCTA AAGATCCCAG CTCTGAAACA GGAACTCGAG TTGGTGTCGT CCAGTACAGT 
CACAACGGAA CATTCCAGGC CATTCGACTC AACGACTCCA AGATCGACTC CATGTCAGCC 
TTCAAAGATG CTGTGAAGAA GCTAGAGTGG ATTGCTGGAG GAACGTGGAC TCCTTCAGCC 
CTGAAGTTCG CCTACGATAA CCTGATTCGT GACAGCCGTC GGTCAAAAGC CAATGTGACG 
GTGGTCGTAA TCACAGATGG TCGGTATGAT CCCCGGGATG ATGACAAACT GCTCAATTAC 
CTCTGCACCG ACACCAGCAT TGATGTCAAC GCTATTGGTA TTGGAGATAT GTTTGACCAG 
CCGGAGGAAA ACGAAAGCTT GAAGTCTATC GCCTGTAGAA AAGATGGAAG AGTCATGGGA 
ATGAGGCGTT TCGCTGATCT GGTCGCAGAA GATTTCATCG ACAGGATTGA GACTGTGCTT 
TGTCCGGATC CTGTGGTCAT TTGCCCTGAT CTTCCATGTA AAAGTGAGCC AGCCGTGGCC 
AACTGCATCC AGCGGCCTGT TGATCTGGTC TTCATGCTGG ACGGCTCAGA GCGTATGGGC 
CAGGAGAATT TCCGCTATGC ACGTGAATTC GTGGAGAATG TGGCCAACCG TCTCACACTA 
GCTCAGGGAG ATGATGATGA ACGCAATGCA AGGGTGGCTC TCCTGCAATA TGGAGATGAA 
AACCAACATC AGTTGGCCTT CAAACTAACA AATAACTTCA CCGTCATCGC TGATGGCCTG 
GCCAACATGA GGTACCTAGA TTCAACCTCC AATGTTGGAA GTGGCATCAT CTACGCCATC 
AACAATATCG TCACAAGCAG AGGCACTCGA TTGGCAAGAC GAAATGCAGA GTTGTCGTTT 
GTCTTCATTA CCGATGGGGT CACCAGTAAC AAAAACCTGG AAGAGGGGAT TAGTGCCATG 
CGCAGAGCCG AGGGCGTTCC CACTGTCATT GCCATGAGCA ATGATGTTGA CAAGGAAATC 
CTTGCAAAGA TTTCCCTGGG AGACCAGACG GCCGTTTTCA GGGGTGAAGA TTTTGCCCGT 
CTGAACAAAG CCAGCTTTTT TGAGCGATTC GTTCGGTGGA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a2 ( XM_009306168.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304443.2 Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X2, mRNA.

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CloneID	ODa54364
Clone ID Related Accession (Same CDS sequence)	XM_009306168.3 , XM_009306168.2
Accession Version	XM_009306168.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306168.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGG ATCCAGACTT CTGTTCAAGC TGGGAGAAGC GCAGAGGAAA CCTCTCCTGC 
AGCTTGGCTT CTCACTCACT CCCTCATTTT GGACTTTATT CTGATTTCTG CTTCTACCTG 
GTTCGGAATG TATGCTTGGT AGTCCTCAAC ATAAGTCATC AGTCGAGAAG TTCTTTAGAT 
TCTCTGGTCT TGGGAAGGAG AGTGAGGATG ATGAGCTTGT CGATCCTCCT CCTGTTGTTT 
GTGGGTGTTC ATGCACAGGG CCAGACTCCA GCCGGCTCTT CGTGTAGTCC TATAAAGAGT 
ACCGAATGCC CGATCGACTT GTACTTTGCT ATTGACACAT CTGAGACCAT CGCTCTTCAG 
GAGAATCCGC CAGGCAGCTT GGTAGAGAGT ATCAAAGATT TCACCATCAG ATTTGCTGAG 
AAGCTCCAAA ATGTGAACTA CAGAGGATCC GTGAACATCA CCTGGGCCAT CGGAGGACTC 
CACTTCTCCC AGCTCCAGGA GTTCTTCAGC ACCATCACCA CTAAAGAGAA GTTCATCAGT 
AATCTCCGGC CCATCAGATA CCTAGGCAGG GGCACGCACA TTGATTGCGC CATCACCAAC 
ATGACCAAGC AGCTGGTCCG CTTTCCATCC AGACCCGATG CCAAACGATT CGCCGTGGTC 
ATCACTGATG GTCACGTGAC TGCGAACCCA TGCGGAGGGA TCAAAGTGGC GGCAGAACGG 
GCACGCGATG AAAATATTAG GATCTTCGCT GTGGCATCGT CCAGGAATTT GGAGGAGACG 
GGACTCAGGG AGATAGCAAA CTCACCCGCT GGAGTTTACC GGGATAAATA CATGGCGGTG 
AATCTCACTG GAACACGGCC GGTCATTGTG ACGTCCACAA TCGATCGCAT TTATGACACA 
ATGCTTCATA TGGCTTACCA AGAGTGCTAC TCTACCAAAT GCTTGGAGAC TCCAGGACCT 
CCAGGTCCCC CAGGACACAG AGGACAGAAA GGAGCTAAAG GAGATAATGG GGGCGCTGGC 
CTTAAAGGTG AAAGGGGACG ACCTGGAGAT CCTGGTATTG AAGGACCCAT TGGACATCCT 
GGTCCTAAAG GAGAGCCTGG TCTCAAAGGA GAAAAGGGAG AAATTGGAGC ACAAGGAAAA 
AAGGGTGTTG CAGGAATAGC TGGCCGTGAT GGAAAAGATG GACAGAAGGG AAAATTTGGC 
AGGATTGGTG CTCCTGGCTG CAAAGGTGAT CCAGGTGACA AGGGTCCTGA TGGTCATCCT 
GGAGATGTTG GAGAGCCTGG TCTTCCAGGC GCTTTGGGAG ATAAGGGTGA TCGAGGTCGT 
CCAGGAAGAT CAGGTCCTCC AGGCCCAGCT GGAGATCCTG GACCAAAAGG AGAGAGAGGA 
AGTCCTGGAT CGCCTGGAAT TCCTGGCACA AAAGGAGGCG CTGGGACAGC TGGAGGACCT 
GGACCCAAAG GAGATGCTGG GCGAAGAGGA GATTTTGGAC CTAAAGGAAG CACTGGACCT 
AATGGGGCAA AAGGAGAGAA GGGTGAACCA GGTGCTGAGG GACCCAGAGG TCTTGCAGGT 
GAAGTTGGCG GCAAAGGATC CAAGGGTGAT AACGGACTTC CAGGACCAAG AGGCCCACCA 
GGAGCTCCTG GAGATGCAGG CAGAAAGGGT TCATCAGGTG ACCCAGGTGA TTCTGGCCCT 
AGAGGTGATC CTGGCCCTCC AGGACCAAAG GGTGATTCTG GCAGACCTGG ATTCAGCTAT 
CCTGGACCAA GAGGACCAAC GGGTGACAGG GGTGAAAAAG GTTCTCCTGG ACCCAGGGGA 
AGCAGAGGAG ACTGTGGGCC AAAGGGTGAC CCAGGTCTAA AGGGAACTCC AGGAGAGCCG 
GGAGAACCTG GCCCAGCAGG AGAGCCTGGA CAGAGGGGTC CTAGAGGAGA TGGAGGAAGA 
GATGGTGATC CTGGACCAGA GGGAGATCCT GGCCTTACTG AATGTGATGT CATGAACTAC 
ATCAGAGAAA CCTGTGGATG CTGTGATTGT GAAAAGAGAT GCGGTGCCCT GGATATTGTG 
TTTGTGATTG ACAGCTCTGA GAGTGTGGGG CTGACCAACT TCACCCTGGA GAAGAATTTT 
GTGATCAACA CCATTAACAG ACTGGGCTCA ATCGCTAAAG ATCCCAGCTC TGAAACAGGA 
ACTCGAGTTG GTGTCGTCCA GTACAGTCAC AACGGAACAT TCCAGGCCAT TCGACTCAAC 
GACTCCAAGA TCGACTCCAT GTCAGCCTTC AAAGATGCTG TGAAGAAGCT AGAGTGGATT 
GCTGGAGGAA CGTGGACTCC TTCAGCCCTG AAGTTCGCCT ACGATAACCT GATTCGTGAC 
AGCCGTCGGT CAAAAGCCAA TGTGACGGTG GTCGTAATCA CAGATGGTCG GTATGATCCC 
CGGGATGATG ACAAACTGCT CAATTACCTC TGCACCGACA CCAGCATTGA TGTCAACGCT 
ATTGGTATTG GAGATATGTT TGACCAGCCG GAGGAAAACG AAAGCTTGAA GTCTATCGCC 
TGTAGAAAAG ATGGAAGAGT CATGGGAATG AGGCGTTTCG CTGATCTGGT CGCAGAAGAT 
TTCATCGACA GGATTGAGAC TGTGCTTTGT CCGGATCCTG TGGTCATTTG CCCTGATCTT 
CCATGTAAAA GTGAGCCAGC CGTGGCCAAC TGCATCCAGC GGCCTGTTGA TCTGGTCTTC 
ATGCTGGACG GCTCAGAGCG TATGGGCCAG GAGAATTTCC GCTATGCACG TGAATTCGTG 
GAGAATGTGG CCAACCGTCT CACACTAGCT CAGGGAGATG ATGATGAACG CAATGCAAGG 
GTGGCTCTCC TGCAATATGG AGATGAAAAC CAACATCAGT TGGCCTTCAA ACTAACAAAT 
AACTTCACCG TCATCGCTGA TGGCCTGGCC AACATGAGGT ACCTAGATTC AACCTCCAAT 
GTTGGAAGTG GCATCATCTA CGCCATCAAC AATATCGTCA CAAGCAGAGG CACTCGATTG 
GCAAGACGAA ATGCAGAGTT GTCGTTTGTC TTCATTACCG ATGGGGTCAC CAGTAACAAA 
AACCTGGAAG AGGGGATTAG TGCCATGCGC AGAGCCGAGG GCGTTCCCAC TGTCATTGCC 
ATGAGCAATG ATGTTGACAA GGAAATCCTT GCAAAGATTT CCCTGGGAGA CCAGACGGCC 
GTTTTCAGGG GTGAAGATTT TGCCCGTCTG AACAAAGCCA GCTTTTTTGA GCGATTCGTT 
CGGTGGATCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304443.2
CDS	16..3270
Translation	MKEDPDFCSSWEKRRGNLSCSLASHSLPHFGLYSDFCFYLVRNVCLVVLNISHQSRSSLDSLVLGRRVRMMSLSILLLLFVGVHAQGQTPAGSSCSPIKSTECPIDLYFAIDTSETIALQENPPGSLVESIKDFTIRFAEKLQNVNYRGSVNITWAIGGLHFSQLQEFFSTITTKEKFISNLRPIRYLGRGTHIDCAITNMTKQLVRFPSRPDAKRFAVVITDGHVTANPCGGIKVAAERARDENIRIFAVASSRNLEETGLREIANSPAGVYRDKYMAVNLTGTRPVIVTSTIDRIYDTMLHMAYQECYSTKCLETPGPPGPPGHRGQKGAKGDNGGAGLKGERGRPGDPGIEGPIGHPGPKGEPGLKGEKGEIGAQGKKGVAGIAGRDGKDGQKGKFGRIGAPGCKGDPGDKGPDGHPGDVGEPGLPGALGDKGDRGRPGRSGPPGPAGDPGPKGERGSPGSPGIPGTKGGAGTAGGPGPKGDAGRRGDFGPKGSTGPNGAKGEKGEPGAEGPRGLAGEVGGKGSKGDNGLPGPRGPPGAPGDAGRKGSSGDPGDSGPRGDPGPPGPKGDSGRPGFSYPGPRGPTGDRGEKGSPGPRGSRGDCGPKGDPGLKGTPGEPGEPGPAGEPGQRGPRGDGGRDGDPGPEGDPGLTECDVMNYIRETCGCCDCEKRCGALDIVFVIDSSESVGLTNFTLEKNFVINTINRLGSIAKDPSSETGTRVGVVQYSHNGTFQAIRLNDSKIDSMSAFKDAVKKLEWIAGGTWTPSALKFAYDNLIRDSRRSKANVTVVVITDGRYDPRDDDKLLNYLCTDTSIDVNAIGIGDMFDQPEENESLKSIACRKDGRVMGMRRFADLVAEDFIDRIETVLCPDPVVICPDLPCKSEPAVANCIQRPVDLVFMLDGSERMGQENFRYAREFVENVANRLTLAQGDDDERNARVALLQYGDENQHQLAFKLTNNFTVIADGLANMRYLDSTSNVGSGIIYAINNIVTSRGTRLARRNAELSFVFITDGVTSNKNLEEGISAMRRAEGVPTVIAMSNDVDKEILAKISLGDQTAVFRGEDFARLNKASFFERFVRWIC

Target ORF information:

RefSeq Version	XM_009306168.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306168.3

ORF Insert Sequence:

ATGAAGGAGG ATCCAGACTT CTGTTCAAGC TGGGAGAAGC GCAGAGGAAA CCTCTCCTGC 
AGCTTGGCTT CTCACTCACT CCCTCATTTT GGACTTTATT CTGATTTCTG CTTCTACCTG 
GTTCGGAATG TATGCTTGGT AGTCCTCAAC ATAAGTCATC AGTCGAGAAG TTCTTTAGAT 
TCTCTGGTCT TGGGAAGGAG AGTGAGGATG ATGAGCTTGT CGATCCTCCT CCTGTTGTTT 
GTGGGTGTTC ATGCACAGGG CCAGACTCCA GCCGGCTCTT CGTGTAGTCC TATAAAGAGT 
ACCGAATGCC CGATCGACTT GTACTTTGCT ATTGACACAT CTGAGACCAT CGCTCTTCAG 
GAGAATCCGC CAGGCAGCTT GGTAGAGAGT ATCAAAGATT TCACCATCAG ATTTGCTGAG 
AAGCTCCAAA ATGTGAACTA CAGAGGATCC GTGAACATCA CCTGGGCCAT CGGAGGACTC 
CACTTCTCCC AGCTCCAGGA GTTCTTCAGC ACCATCACCA CTAAAGAGAA GTTCATCAGT 
AATCTCCGGC CCATCAGATA CCTAGGCAGG GGCACGCACA TTGATTGCGC CATCACCAAC 
ATGACCAAGC AGCTGGTCCG CTTTCCATCC AGACCCGATG CCAAACGATT CGCCGTGGTC 
ATCACTGATG GTCACGTGAC TGCGAACCCA TGCGGAGGGA TCAAAGTGGC GGCAGAACGG 
GCACGCGATG AAAATATTAG GATCTTCGCT GTGGCATCGT CCAGGAATTT GGAGGAGACG 
GGACTCAGGG AGATAGCAAA CTCACCCGCT GGAGTTTACC GGGATAAATA CATGGCGGTG 
AATCTCACTG GAACACGGCC GGTCATTGTG ACGTCCACAA TCGATCGCAT TTATGACACA 
ATGCTTCATA TGGCTTACCA AGAGTGCTAC TCTACCAAAT GCTTGGAGAC TCCAGGACCT 
CCAGGTCCCC CAGGACACAG AGGACAGAAA GGAGCTAAAG GAGATAATGG GGGCGCTGGC 
CTTAAAGGTG AAAGGGGACG ACCTGGAGAT CCTGGTATTG AAGGACCCAT TGGACATCCT 
GGTCCTAAAG GAGAGCCTGG TCTCAAAGGA GAAAAGGGAG AAATTGGAGC ACAAGGAAAA 
AAGGGTGTTG CAGGAATAGC TGGCCGTGAT GGAAAAGATG GACAGAAGGG AAAATTTGGC 
AGGATTGGTG CTCCTGGCTG CAAAGGTGAT CCAGGTGACA AGGGTCCTGA TGGTCATCCT 
GGAGATGTTG GAGAGCCTGG TCTTCCAGGC GCTTTGGGAG ATAAGGGTGA TCGAGGTCGT 
CCAGGAAGAT CAGGTCCTCC AGGCCCAGCT GGAGATCCTG GACCAAAAGG AGAGAGAGGA 
AGTCCTGGAT CGCCTGGAAT TCCTGGCACA AAAGGAGGCG CTGGGACAGC TGGAGGACCT 
GGACCCAAAG GAGATGCTGG GCGAAGAGGA GATTTTGGAC CTAAAGGAAG CACTGGACCT 
AATGGGGCAA AAGGAGAGAA GGGTGAACCA GGTGCTGAGG GACCCAGAGG TCTTGCAGGT 
GAAGTTGGCG GCAAAGGATC CAAGGGTGAT AACGGACTTC CAGGACCAAG AGGCCCACCA 
GGAGCTCCTG GAGATGCAGG CAGAAAGGGT TCATCAGGTG ACCCAGGTGA TTCTGGCCCT 
AGAGGTGATC CTGGCCCTCC AGGACCAAAG GGTGATTCTG GCAGACCTGG ATTCAGCTAT 
CCTGGACCAA GAGGACCAAC GGGTGACAGG GGTGAAAAAG GTTCTCCTGG ACCCAGGGGA 
AGCAGAGGAG ACTGTGGGCC AAAGGGTGAC CCAGGTCTAA AGGGAACTCC AGGAGAGCCG 
GGAGAACCTG GCCCAGCAGG AGAGCCTGGA CAGAGGGGTC CTAGAGGAGA TGGAGGAAGA 
GATGGTGATC CTGGACCAGA GGGAGATCCT GGCCTTACTG AATGTGATGT CATGAACTAC 
ATCAGAGAAA CCTGTGGATG CTGTGATTGT GAAAAGAGAT GCGGTGCCCT GGATATTGTG 
TTTGTGATTG ACAGCTCTGA GAGTGTGGGG CTGACCAACT TCACCCTGGA GAAGAATTTT 
GTGATCAACA CCATTAACAG ACTGGGCTCA ATCGCTAAAG ATCCCAGCTC TGAAACAGGA 
ACTCGAGTTG GTGTCGTCCA GTACAGTCAC AACGGAACAT TCCAGGCCAT TCGACTCAAC 
GACTCCAAGA TCGACTCCAT GTCAGCCTTC AAAGATGCTG TGAAGAAGCT AGAGTGGATT 
GCTGGAGGAA CGTGGACTCC TTCAGCCCTG AAGTTCGCCT ACGATAACCT GATTCGTGAC 
AGCCGTCGGT CAAAAGCCAA TGTGACGGTG GTCGTAATCA CAGATGGTCG GTATGATCCC 
CGGGATGATG ACAAACTGCT CAATTACCTC TGCACCGACA CCAGCATTGA TGTCAACGCT 
ATTGGTATTG GAGATATGTT TGACCAGCCG GAGGAAAACG AAAGCTTGAA GTCTATCGCC 
TGTAGAAAAG ATGGAAGAGT CATGGGAATG AGGCGTTTCG CTGATCTGGT CGCAGAAGAT 
TTCATCGACA GGATTGAGAC TGTGCTTTGT CCGGATCCTG TGGTCATTTG CCCTGATCTT 
CCATGTAAAA GTGAGCCAGC CGTGGCCAAC TGCATCCAGC GGCCTGTTGA TCTGGTCTTC 
ATGCTGGACG GCTCAGAGCG TATGGGCCAG GAGAATTTCC GCTATGCACG TGAATTCGTG 
GAGAATGTGG CCAACCGTCT CACACTAGCT CAGGGAGATG ATGATGAACG CAATGCAAGG 
GTGGCTCTCC TGCAATATGG AGATGAAAAC CAACATCAGT TGGCCTTCAA ACTAACAAAT 
AACTTCACCG TCATCGCTGA TGGCCTGGCC AACATGAGGT ACCTAGATTC AACCTCCAAT 
GTTGGAAGTG GCATCATCTA CGCCATCAAC AATATCGTCA CAAGCAGAGG CACTCGATTG 
GCAAGACGAA ATGCAGAGTT GTCGTTTGTC TTCATTACCG ATGGGGTCAC CAGTAACAAA 
AACCTGGAAG AGGGGATTAG TGCCATGCGC AGAGCCGAGG GCGTTCCCAC TGTCATTGCC 
ATGAGCAATG ATGTTGACAA GGAAATCCTT GCAAAGATTT CCCTGGGAGA CCAGACGGCC 
GTTTTCAGGG GTGAAGATTT TGCCCGTCTG AACAAAGCCA GCTTTTTTGA GCGATTCGTT 
CGGTGGATCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a2 ( XM_009306168.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304443.2 Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54364
Clone ID Related Accession (Same CDS sequence)	XM_009306168.3 , XM_009306168.2
Accession Version	XM_009306168.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(VI) chain isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGG ATCCAGACTT CTGTTCAAGC TGGGAGAAGC GCAGAGGAAA CCTCTCCTGC 
AGCTTGGCTT CTCACTCACT CCCTCATTTT GGACTTTATT CTGATTTCTG CTTCTACCTG 
GTTCGGAATG TATGCTTGGT AGTCCTCAAC ATAAGTCATC AGTCGAGAAG TTCTTTAGAT 
TCTCTGGTCT TGGGAAGGAG AGTGAGGATG ATGAGCTTGT CGATCCTCCT CCTGTTGTTT 
GTGGGTGTTC ATGCACAGGG CCAGACTCCA GCCGGCTCTT CGTGTAGTCC TATAAAGAGT 
ACCGAATGCC CGATCGACTT GTACTTTGCT ATTGACACAT CTGAGACCAT CGCTCTTCAG 
GAGAATCCGC CAGGCAGCTT GGTAGAGAGT ATCAAAGATT TCACCATCAG ATTTGCTGAG 
AAGCTCCAAA ATGTGAACTA CAGAGGATCC GTGAACATCA CCTGGGCCAT CGGAGGACTC 
CACTTCTCCC AGCTCCAGGA GTTCTTCAGC ACCATCACCA CTAAAGAGAA GTTCATCAGT 
AATCTCCGGC CCATCAGATA CCTAGGCAGG GGCACGCACA TTGATTGCGC CATCACCAAC 
ATGACCAAGC AGCTGGTCCG CTTTCCATCC AGACCCGATG CCAAACGATT CGCCGTGGTC 
ATCACTGATG GTCACGTGAC TGCGAACCCA TGCGGAGGGA TCAAAGTGGC GGCAGAACGG 
GCACGCGATG AAAATATTAG GATCTTCGCT GTGGCATCGT CCAGGAATTT GGAGGAGACG 
GGACTCAGGG AGATAGCAAA CTCACCCGCT GGAGTTTACC GGGATAAATA CATGGCGGTG 
AATCTCACTG GAACACGGCC GGTCATTGTG ACGTCCACAA TCGATCGCAT TTATGACACA 
ATGCTTCATA TGGCTTACCA AGAGTGCTAC TCTACCAAAT GCTTGGAGAC TCCAGGACCT 
CCAGGTCCCC CAGGACACAG AGGACAGAAA GGAGCTAAAG GAGATAATGG GGGCGCTGGC 
CTTAAAGGTG AAAGGGGACG ACCTGGAGAT CCTGGTATTG AAGGACCCAT TGGACATCCT 
GGTCCTAAAG GAGAGCCTGG TCTCAAAGGA GAAAAGGGAG AAATTGGAGC ACAAGGAAAA 
AAGGGTGTTG CAGGAATAGC TGGCCGTGAT GGAAAAGATG GACAGAAGGG AAAATTTGGC 
AGGATTGGTG CTCCTGGCTG CAAAGGTGAT CCAGGTGACA AGGGTCCTGA TGGTCATCCT 
GGAGATGTTG GAGAGCCTGG TCTTCCAGGC GCTTTGGGAG ATAAGGGTGA TCGAGGTCGT 
CCAGGAAGAT CAGGTCCTCC AGGCCCAGCT GGAGATCCTG GACCAAAAGG AGAGAGAGGA 
AGTCCTGGAT CGCCTGGAAT TCCTGGCACA AAAGGAGGCG CTGGGACAGC TGGAGGACCT 
GGACCCAAAG GAGATGCTGG GCGAAGAGGA GATTTTGGAC CTAAAGGAAG CACTGGACCT 
AATGGGGCAA AAGGAGAGAA GGGTGAACCA GGTGCTGAGG GACCCAGAGG TCTTGCAGGT 
GAAGTTGGCG GCAAAGGATC CAAGGGTGAT AACGGACTTC CAGGACCAAG AGGCCCACCA 
GGAGCTCCTG GAGATGCAGG CAGAAAGGGT TCATCAGGTG ACCCAGGTGA TTCTGGCCCT 
AGAGGTGATC CTGGCCCTCC AGGACCAAAG GGTGATTCTG GCAGACCTGG ATTCAGCTAT 
CCTGGACCAA GAGGACCAAC GGGTGACAGG GGTGAAAAAG GTTCTCCTGG ACCCAGGGGA 
AGCAGAGGAG ACTGTGGGCC AAAGGGTGAC CCAGGTCTAA AGGGAACTCC AGGAGAGCCG 
GGAGAACCTG GCCCAGCAGG AGAGCCTGGA CAGAGGGGTC CTAGAGGAGA TGGAGGAAGA 
GATGGTGATC CTGGACCAGA GGGAGATCCT GGCCTTACTG AATGTGATGT CATGAACTAC 
ATCAGAGAAA CCTGTGGATG CTGTGATTGT GAAAAGAGAT GCGGTGCCCT GGATATTGTG 
TTTGTGATTG ACAGCTCTGA GAGTGTGGGG CTGACCAACT TCACCCTGGA GAAGAATTTT 
GTGATCAACA CCATTAACAG ACTGGGCTCA ATCGCTAAAG ATCCCAGCTC TGAAACAGGA 
ACTCGAGTTG GTGTCGTCCA GTACAGTCAC AACGGAACAT TCCAGGCCAT TCGACTCAAC 
GACTCCAAGA TCGACTCCAT GTCAGCCTTC AAAGATGCTG TGAAGAAGCT AGAGTGGATT 
GCTGGAGGAA CGTGGACTCC TTCAGCCCTG AAGTTCGCCT ACGATAACCT GATTCGTGAC 
AGCCGTCGGT CAAAAGCCAA TGTGACGGTG GTCGTAATCA CAGATGGTCG GTATGATCCC 
CGGGATGATG ACAAACTGCT CAATTACCTC TGCACCGACA CCAGCATTGA TGTCAACGCT 
ATTGGTATTG GAGATATGTT TGACCAGCCG GAGGAAAACG AAAGCTTGAA GTCTATCGCC 
TGTAGAAAAG ATGGAAGAGT CATGGGAATG AGGCGTTTCG CTGATCTGGT CGCAGAAGAT 
TTCATCGACA GGATTGAGAC TGTGCTTTGT CCGGATCCTG TGGTCATTTG CCCTGATCTT 
CCATGTAAAA GTGAGCCAGC CGTGGCCAAC TGCATCCAGC GGCCTGTTGA TCTGGTCTTC 
ATGCTGGACG GCTCAGAGCG TATGGGCCAG GAGAATTTCC GCTATGCACG TGAATTCGTG 
GAGAATGTGG CCAACCGTCT CACACTAGCT CAGGGAGATG ATGATGAACG CAATGCAAGG 
GTGGCTCTCC TGCAATATGG AGATGAAAAC CAACATCAGT TGGCCTTCAA ACTAACAAAT 
AACTTCACCG TCATCGCTGA TGGCCTGGCC AACATGAGGT ACCTAGATTC AACCTCCAAT 
GTTGGAAGTG GCATCATCTA CGCCATCAAC AATATCGTCA CAAGCAGAGG CACTCGATTG 
GCAAGACGAA ATGCAGAGTT GTCGTTTGTC TTCATTACCG ATGGGGTCAC CAGTAACAAA 
AACCTGGAAG AGGGGATTAG TGCCATGCGC AGAGCCGAGG GCGTTCCCAC TGTCATTGCC 
ATGAGCAATG ATGTTGACAA GGAAATCCTT GCAAAGATTT CCCTGGGAGA CCAGACGGCC 
GTTTTCAGGG GTGAAGATTT TGCCCGTCTG AACAAAGCCA GCTTTTTTGA GCGATTCGTT 
CGGTGGATCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304443.2
CDS	16..3270
Translation	MKEDPDFCSSWEKRRGNLSCSLASHSLPHFGLYSDFCFYLVRNVCLVVLNISHQSRSSLDSLVLGRRVRMMSLSILLLLFVGVHAQGQTPAGSSCSPIKSTECPIDLYFAIDTSETIALQENPPGSLVESIKDFTIRFAEKLQNVNYRGSVNITWAIGGLHFSQLQEFFSTITTKEKFISNLRPIRYLGRGTHIDCAITNMTKQLVRFPSRPDAKRFAVVITDGHVTANPCGGIKVAAERARDENIRIFAVASSRNLEETGLREIANSPAGVYRDKYMAVNLTGTRPVIVTSTIDRIYDTMLHMAYQECYSTKCLETPGPPGPPGHRGQKGAKGDNGGAGLKGERGRPGDPGIEGPIGHPGPKGEPGLKGEKGEIGAQGKKGVAGIAGRDGKDGQKGKFGRIGAPGCKGDPGDKGPDGHPGDVGEPGLPGALGDKGDRGRPGRSGPPGPAGDPGPKGERGSPGSPGIPGTKGGAGTAGGPGPKGDAGRRGDFGPKGSTGPNGAKGEKGEPGAEGPRGLAGEVGGKGSKGDNGLPGPRGPPGAPGDAGRKGSSGDPGDSGPRGDPGPPGPKGDSGRPGFSYPGPRGPTGDRGEKGSPGPRGSRGDCGPKGDPGLKGTPGEPGEPGPAGEPGQRGPRGDGGRDGDPGPEGDPGLTECDVMNYIRETCGCCDCEKRCGALDIVFVIDSSESVGLTNFTLEKNFVINTINRLGSIAKDPSSETGTRVGVVQYSHNGTFQAIRLNDSKIDSMSAFKDAVKKLEWIAGGTWTPSALKFAYDNLIRDSRRSKANVTVVVITDGRYDPRDDDKLLNYLCTDTSIDVNAIGIGDMFDQPEENESLKSIACRKDGRVMGMRRFADLVAEDFIDRIETVLCPDPVVICPDLPCKSEPAVANCIQRPVDLVFMLDGSERMGQENFRYAREFVENVANRLTLAQGDDDERNARVALLQYGDENQHQLAFKLTNNFTVIADGLANMRYLDSTSNVGSGIIYAINNIVTSRGTRLARRNAELSFVFITDGVTSNKNLEEGISAMRRAEGVPTVIAMSNDVDKEILAKISLGDQTAVFRGEDFARLNKASFFERFVRWIC

Target ORF information:

RefSeq Version	XM_009306168.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type VI, alpha 2 (col6a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306168.2

ORF Insert Sequence:

ATGAAGGAGG ATCCAGACTT CTGTTCAAGC TGGGAGAAGC GCAGAGGAAA CCTCTCCTGC 
AGCTTGGCTT CTCACTCACT CCCTCATTTT GGACTTTATT CTGATTTCTG CTTCTACCTG 
GTTCGGAATG TATGCTTGGT AGTCCTCAAC ATAAGTCATC AGTCGAGAAG TTCTTTAGAT 
TCTCTGGTCT TGGGAAGGAG AGTGAGGATG ATGAGCTTGT CGATCCTCCT CCTGTTGTTT 
GTGGGTGTTC ATGCACAGGG CCAGACTCCA GCCGGCTCTT CGTGTAGTCC TATAAAGAGT 
ACCGAATGCC CGATCGACTT GTACTTTGCT ATTGACACAT CTGAGACCAT CGCTCTTCAG 
GAGAATCCGC CAGGCAGCTT GGTAGAGAGT ATCAAAGATT TCACCATCAG ATTTGCTGAG 
AAGCTCCAAA ATGTGAACTA CAGAGGATCC GTGAACATCA CCTGGGCCAT CGGAGGACTC 
CACTTCTCCC AGCTCCAGGA GTTCTTCAGC ACCATCACCA CTAAAGAGAA GTTCATCAGT 
AATCTCCGGC CCATCAGATA CCTAGGCAGG GGCACGCACA TTGATTGCGC CATCACCAAC 
ATGACCAAGC AGCTGGTCCG CTTTCCATCC AGACCCGATG CCAAACGATT CGCCGTGGTC 
ATCACTGATG GTCACGTGAC TGCGAACCCA TGCGGAGGGA TCAAAGTGGC GGCAGAACGG 
GCACGCGATG AAAATATTAG GATCTTCGCT GTGGCATCGT CCAGGAATTT GGAGGAGACG 
GGACTCAGGG AGATAGCAAA CTCACCCGCT GGAGTTTACC GGGATAAATA CATGGCGGTG 
AATCTCACTG GAACACGGCC GGTCATTGTG ACGTCCACAA TCGATCGCAT TTATGACACA 
ATGCTTCATA TGGCTTACCA AGAGTGCTAC TCTACCAAAT GCTTGGAGAC TCCAGGACCT 
CCAGGTCCCC CAGGACACAG AGGACAGAAA GGAGCTAAAG GAGATAATGG GGGCGCTGGC 
CTTAAAGGTG AAAGGGGACG ACCTGGAGAT CCTGGTATTG AAGGACCCAT TGGACATCCT 
GGTCCTAAAG GAGAGCCTGG TCTCAAAGGA GAAAAGGGAG AAATTGGAGC ACAAGGAAAA 
AAGGGTGTTG CAGGAATAGC TGGCCGTGAT GGAAAAGATG GACAGAAGGG AAAATTTGGC 
AGGATTGGTG CTCCTGGCTG CAAAGGTGAT CCAGGTGACA AGGGTCCTGA TGGTCATCCT 
GGAGATGTTG GAGAGCCTGG TCTTCCAGGC GCTTTGGGAG ATAAGGGTGA TCGAGGTCGT 
CCAGGAAGAT CAGGTCCTCC AGGCCCAGCT GGAGATCCTG GACCAAAAGG AGAGAGAGGA 
AGTCCTGGAT CGCCTGGAAT TCCTGGCACA AAAGGAGGCG CTGGGACAGC TGGAGGACCT 
GGACCCAAAG GAGATGCTGG GCGAAGAGGA GATTTTGGAC CTAAAGGAAG CACTGGACCT 
AATGGGGCAA AAGGAGAGAA GGGTGAACCA GGTGCTGAGG GACCCAGAGG TCTTGCAGGT 
GAAGTTGGCG GCAAAGGATC CAAGGGTGAT AACGGACTTC CAGGACCAAG AGGCCCACCA 
GGAGCTCCTG GAGATGCAGG CAGAAAGGGT TCATCAGGTG ACCCAGGTGA TTCTGGCCCT 
AGAGGTGATC CTGGCCCTCC AGGACCAAAG GGTGATTCTG GCAGACCTGG ATTCAGCTAT 
CCTGGACCAA GAGGACCAAC GGGTGACAGG GGTGAAAAAG GTTCTCCTGG ACCCAGGGGA 
AGCAGAGGAG ACTGTGGGCC AAAGGGTGAC CCAGGTCTAA AGGGAACTCC AGGAGAGCCG 
GGAGAACCTG GCCCAGCAGG AGAGCCTGGA CAGAGGGGTC CTAGAGGAGA TGGAGGAAGA 
GATGGTGATC CTGGACCAGA GGGAGATCCT GGCCTTACTG AATGTGATGT CATGAACTAC 
ATCAGAGAAA CCTGTGGATG CTGTGATTGT GAAAAGAGAT GCGGTGCCCT GGATATTGTG 
TTTGTGATTG ACAGCTCTGA GAGTGTGGGG CTGACCAACT TCACCCTGGA GAAGAATTTT 
GTGATCAACA CCATTAACAG ACTGGGCTCA ATCGCTAAAG ATCCCAGCTC TGAAACAGGA 
ACTCGAGTTG GTGTCGTCCA GTACAGTCAC AACGGAACAT TCCAGGCCAT TCGACTCAAC 
GACTCCAAGA TCGACTCCAT GTCAGCCTTC AAAGATGCTG TGAAGAAGCT AGAGTGGATT 
GCTGGAGGAA CGTGGACTCC TTCAGCCCTG AAGTTCGCCT ACGATAACCT GATTCGTGAC 
AGCCGTCGGT CAAAAGCCAA TGTGACGGTG GTCGTAATCA CAGATGGTCG GTATGATCCC 
CGGGATGATG ACAAACTGCT CAATTACCTC TGCACCGACA CCAGCATTGA TGTCAACGCT 
ATTGGTATTG GAGATATGTT TGACCAGCCG GAGGAAAACG AAAGCTTGAA GTCTATCGCC 
TGTAGAAAAG ATGGAAGAGT CATGGGAATG AGGCGTTTCG CTGATCTGGT CGCAGAAGAT 
TTCATCGACA GGATTGAGAC TGTGCTTTGT CCGGATCCTG TGGTCATTTG CCCTGATCTT 
CCATGTAAAA GTGAGCCAGC CGTGGCCAAC TGCATCCAGC GGCCTGTTGA TCTGGTCTTC 
ATGCTGGACG GCTCAGAGCG TATGGGCCAG GAGAATTTCC GCTATGCACG TGAATTCGTG 
GAGAATGTGG CCAACCGTCT CACACTAGCT CAGGGAGATG ATGATGAACG CAATGCAAGG 
GTGGCTCTCC TGCAATATGG AGATGAAAAC CAACATCAGT TGGCCTTCAA ACTAACAAAT 
AACTTCACCG TCATCGCTGA TGGCCTGGCC AACATGAGGT ACCTAGATTC AACCTCCAAT 
GTTGGAAGTG GCATCATCTA CGCCATCAAC AATATCGTCA CAAGCAGAGG CACTCGATTG 
GCAAGACGAA ATGCAGAGTT GTCGTTTGTC TTCATTACCG ATGGGGTCAC CAGTAACAAA 
AACCTGGAAG AGGGGATTAG TGCCATGCGC AGAGCCGAGG GCGTTCCCAC TGTCATTGCC 
ATGAGCAATG ATGTTGACAA GGAAATCCTT GCAAAGATTT CCCTGGGAGA CCAGACGGCC 
GTTTTCAGGG GTGAAGATTT TGCCCGTCTG AACAAAGCCA GCTTTTTTGA GCGATTCGTT 
CGGTGGATCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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col6a2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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